AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52335


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52335.t1 MTR_7g084070 94.949 297 10 2 1 292 105 401 0.0 565
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52335.t1 AT5G13970 35.276 326 147 15 1 289 105 403 1.00e-38 140
MS.gene52335.t1 AT5G13310 32.530 249 137 8 44 286 143 366 1.37e-19 88.2
MS.gene52335.t1 AT5G13310 32.530 249 137 8 44 286 143 366 1.37e-19 88.2

Find 76 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAACCGAACACGTTTAT+TGG 0.185704 7.3:+24508060 None:intergenic
AGTTTCCTTGCACAGATAAA+AGG 0.269056 7.3:-24507812 MS.gene52335:CDS
CATGGATGACAAAGCAAATA+AGG 0.282071 7.3:-24507846 MS.gene52335:CDS
AGATTTCTTCTTCGGTATAA+AGG 0.324848 7.3:+24507739 None:intergenic
TCATCGGCAGCTATGCTAAC+AGG 0.343183 7.3:+24507650 None:intergenic
TCAAACCGAACACGTTTATT+GGG 0.352578 7.3:+24508061 None:intergenic
GAAGGATTGCGAATATAATC+AGG 0.355445 7.3:+24507905 None:intergenic
GAAATCTGGTGATGTCACTA+TGG 0.375778 7.3:-24507723 MS.gene52335:CDS
AGGCTCTTGGCAAAGAGGGC+AGG 0.383943 7.3:-24508336 MS.gene52335:CDS
GCAAATTCCTGTGACTTTGA+TGG 0.394108 7.3:+24507938 None:intergenic
GGGATAAGTTCACGTGTGTT+TGG 0.397330 7.3:-24508271 MS.gene52335:CDS
ATGCAAAGTATCAATTTGAT+TGG 0.400728 7.3:+24508177 None:intergenic
CTCTTGGCAAAGAGGGCAGG+GGG 0.408776 7.3:-24508333 MS.gene52335:CDS
TGCAATGGAGGAGGATGAAC+AGG 0.420285 7.3:-24507603 MS.gene52335:CDS
GAGATAAATGAAGATGGAAT+CGG 0.420742 7.3:-24508292 MS.gene52335:CDS
ACATCACCAGATTTCTTCTT+CGG 0.424129 7.3:+24507731 None:intergenic
GGCTCTTGGCAAAGAGGGCA+GGG 0.430694 7.3:-24508335 MS.gene52335:CDS
GGAGAGAATAAGCTTGAAGT+TGG 0.439007 7.3:-24507701 MS.gene52335:CDS
CGGTTTGATTCTGATAAAGA+TGG 0.442882 7.3:-24508046 MS.gene52335:CDS
TTTATACCGAAGAAGAAATC+TGG 0.445367 7.3:-24507737 MS.gene52335:CDS
TTACTCTTCAAAATTGACTT+GGG 0.458046 7.3:+24508106 None:intergenic
GGAGGATCATTATGACAAAC+AGG 0.458179 7.3:-24508356 MS.gene52335:CDS
GAAGAAAACCGACGAGGACT+TGG 0.459863 7.3:-24507528 MS.gene52335:CDS
GCTCTTGGCAAAGAGGGCAG+GGG 0.470014 7.3:-24508334 MS.gene52335:CDS
AGATAAATGAAGATGGAATC+GGG 0.470704 7.3:-24508291 MS.gene52335:CDS
ATCACAGGCAGATGAAGCTT+TGG 0.495942 7.3:-24507780 MS.gene52335:CDS
CATTATGACAAACAGGCTCT+TGG 0.497963 7.3:-24508349 MS.gene52335:CDS
TTTACTCTTCAAAATTGACT+TGG 0.500079 7.3:+24508105 None:intergenic
GCATCACCACCACCGACGTC+TGG 0.501233 7.3:+24508136 None:intergenic
GAATAAGCTTGAAGTTGGAA+AGG 0.511820 7.3:-24507696 MS.gene52335:CDS
CTAGGCCCAATAAACGTGTT+CGG 0.516488 7.3:-24508066 MS.gene52335:CDS
AGAAGTTGAACCGCAAGTAC+AGG 0.519023 7.3:-24507550 MS.gene52335:CDS
AGCTGCATCCTCTTCCATTG+AGG 0.521556 7.3:+24507970 None:intergenic
AAGATAATCCTTCAGAGTCT+AGG 0.526294 7.3:-24508084 MS.gene52335:CDS
TGCTAGGATTAAGTTGAAGC+AGG 0.527169 7.3:-24508212 MS.gene52335:CDS
AAAAGGTTCAAATGCATCAC+AGG 0.530303 7.3:-24507795 MS.gene52335:CDS
ATGAGGGAGATAAATGAAGA+TGG 0.530531 7.3:-24508298 MS.gene52335:CDS
AGGATGAGAAGGAAGGAACC+AGG 0.531038 7.3:-24508012 MS.gene52335:CDS
GGTCTTCATTCGAACATCCC+TGG 0.538346 7.3:+24507994 None:intergenic
GAAGACCTCCTCAATGGAAG+AGG 0.542891 7.3:-24507978 MS.gene52335:CDS
TCGAATGAAGACCTCCTCAA+TGG 0.542894 7.3:-24507984 MS.gene52335:CDS
TCTTGGCAAAGAGGGCAGGG+GGG 0.547481 7.3:-24508332 MS.gene52335:CDS
GAAAACCGACGAGGACTTGG+AGG 0.553003 7.3:-24507525 MS.gene52335:CDS
AAATCAGCCATCAAAGTCAC+AGG 0.553173 7.3:-24507945 MS.gene52335:CDS
TTCGGTATAAAGGTAACAGA+TGG 0.555047 7.3:+24507749 None:intergenic
AAATGATGACGAGGATGAGA+AGG 0.559814 7.3:-24508023 MS.gene52335:CDS
AAACAGGCTCTTGGCAAAGA+GGG 0.560590 7.3:-24508340 MS.gene52335:CDS
GAATCACTTTATGCAGGATG+AGG 0.571464 7.3:-24508377 None:intergenic
TCACTTTATGCAGGATGAGG+AGG 0.583527 7.3:-24508374 None:intergenic
AAATCTGGTGATGTCACTAT+GGG 0.584932 7.3:-24507722 MS.gene52335:CDS
CATCGGCAGCTATGCTAACA+GGG 0.589427 7.3:+24507651 None:intergenic
TTATTGGGCCTAGACTCTGA+AGG 0.592184 7.3:+24508076 None:intergenic
CAAACAGGCTCTTGGCAAAG+AGG 0.593885 7.3:-24508341 MS.gene52335:CDS
GGCAGCTATGCTAACAGGGA+AGG 0.594716 7.3:+24507655 None:intergenic
TGCATCCTCTTCCATTGAGG+AGG 0.595415 7.3:+24507973 None:intergenic
TACTCTTCAAAATTGACTTG+GGG 0.595453 7.3:+24508107 None:intergenic
CCTACACCAGACGTCGGTGG+TGG 0.598318 7.3:-24508142 MS.gene52335:CDS
GGGCTAATCATACCGCTGCT+AGG 0.601553 7.3:-24508228 MS.gene52335:CDS
CAGCGGTATGATTAGCCCGT+GGG 0.608205 7.3:+24508233 None:intergenic
TGATGTATGTGCAATGGAGG+AGG 0.619859 7.3:-24507612 MS.gene52335:CDS
AGGCTCCTCCAAGTCCTCGT+CGG 0.620365 7.3:+24507520 None:intergenic
GCAGCGGTATGATTAGCCCG+TGG 0.621262 7.3:+24508232 None:intergenic
CCACCACCGACGTCTGGTGT+AGG 0.628069 7.3:+24508142 None:intergenic
GTATAAAGGTAACAGATGGA+AGG 0.634228 7.3:+24507753 None:intergenic
GATGACGAGGATGAGAAGGA+AGG 0.638055 7.3:-24508019 MS.gene52335:CDS
CAGTGATGTATGTGCAATGG+AGG 0.643095 7.3:-24507615 MS.gene52335:CDS
GGATGAACAGGAAGTTATCG+AGG 0.649044 7.3:-24507591 MS.gene52335:CDS
AAACAGTGATGTATGTGCAA+TGG 0.651519 7.3:-24507618 MS.gene52335:CDS
TTCCAAACATGCTACACAGT+AGG 0.652049 7.3:+24507500 None:intergenic
GGATGAGAAGGAAGGAACCA+GGG 0.666124 7.3:-24508011 MS.gene52335:CDS
GGATGACAAAGCAAATAAGG+AGG 0.689367 7.3:-24507843 MS.gene52335:CDS
CTTCAACTTAATCCTAGCAG+CGG 0.691169 7.3:+24508216 None:intergenic
AAACCTACACCAGACGTCGG+TGG 0.708641 7.3:-24508145 MS.gene52335:CDS
GAGAAACCTACACCAGACGT+CGG 0.745038 7.3:-24508148 MS.gene52335:CDS
TAAAGATGGAAATGATGACG+AGG 0.752226 7.3:-24508032 MS.gene52335:CDS
GTACAGGAAGAAAACCGACG+AGG 0.795841 7.3:-24507534 MS.gene52335:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTTACTCTTCAAAATTGACT+TGG + chr7.3:24507776-24507795 None:intergenic 25.0%
!! ATGCAAAGTATCAATTTGAT+TGG + chr7.3:24507704-24507723 None:intergenic 25.0%
!! TTACTCTTCAAAATTGACTT+GGG + chr7.3:24507775-24507794 None:intergenic 25.0%
!!! GTATAATGTGTGTATTTTGA+AGG + chr7.3:24507994-24508013 None:intergenic 25.0%
AGATAAATGAAGATGGAATC+GGG - chr7.3:24507587-24507606 MS.gene52335:CDS 30.0%
AGATTTCTTCTTCGGTATAA+AGG + chr7.3:24508142-24508161 None:intergenic 30.0%
GAGATAAATGAAGATGGAAT+CGG - chr7.3:24507586-24507605 MS.gene52335:CDS 30.0%
TTTATACCGAAGAAGAAATC+TGG - chr7.3:24508141-24508160 MS.gene52335:CDS 30.0%
!! TACTCTTCAAAATTGACTTG+GGG + chr7.3:24507774-24507793 None:intergenic 30.0%
!! TTTTGATTCTTCAACTGACA+TGG - chr7.3:24508014-24508033 MS.gene52335:CDS 30.0%
AAAAGGTTCAAATGCATCAC+AGG - chr7.3:24508083-24508102 MS.gene52335:CDS 35.0%
AAACAGTGATGTATGTGCAA+TGG - chr7.3:24508260-24508279 MS.gene52335:CDS 35.0%
AAGATAATCCTTCAGAGTCT+AGG - chr7.3:24507794-24507813 MS.gene52335:CDS 35.0%
ACATCACCAGATTTCTTCTT+CGG + chr7.3:24508150-24508169 None:intergenic 35.0%
AGTTTCCTTGCACAGATAAA+AGG - chr7.3:24508066-24508085 MS.gene52335:CDS 35.0%
ATCAAACCGAACACGTTTAT+TGG + chr7.3:24507821-24507840 None:intergenic 35.0%
ATGAGGGAGATAAATGAAGA+TGG - chr7.3:24507580-24507599 MS.gene52335:CDS 35.0%
CATGGATGACAAAGCAAATA+AGG - chr7.3:24508032-24508051 MS.gene52335:CDS 35.0%
CGGTTTGATTCTGATAAAGA+TGG - chr7.3:24507832-24507851 MS.gene52335:CDS 35.0%
GAAGGATTGCGAATATAATC+AGG + chr7.3:24507976-24507995 None:intergenic 35.0%
GAATAAGCTTGAAGTTGGAA+AGG - chr7.3:24508182-24508201 MS.gene52335:CDS 35.0%
GTATAAAGGTAACAGATGGA+AGG + chr7.3:24508128-24508147 None:intergenic 35.0%
TCAAACCGAACACGTTTATT+GGG + chr7.3:24507820-24507839 None:intergenic 35.0%
TTCGGTATAAAGGTAACAGA+TGG + chr7.3:24508132-24508151 None:intergenic 35.0%
! AAATCTGGTGATGTCACTAT+GGG - chr7.3:24508156-24508175 MS.gene52335:CDS 35.0%
! ACTGTTTTCAGTGTCATCAT+CGG + chr7.3:24508247-24508266 None:intergenic 35.0%
! TAAAGATGGAAATGATGACG+AGG - chr7.3:24507846-24507865 MS.gene52335:CDS 35.0%
! TTGAACCTTTTATCTGTGCA+AGG + chr7.3:24508074-24508093 None:intergenic 35.0%
AAATCAGCCATCAAAGTCAC+AGG - chr7.3:24507933-24507952 MS.gene52335:CDS 40.0%
CATTATGACAAACAGGCTCT+TGG - chr7.3:24507529-24507548 MS.gene52335:CDS 40.0%
CTTCAACTTAATCCTAGCAG+CGG + chr7.3:24507665-24507684 None:intergenic 40.0%
GCAAATTCCTGTGACTTTGA+TGG + chr7.3:24507943-24507962 None:intergenic 40.0%
GGAGAGAATAAGCTTGAAGT+TGG - chr7.3:24508177-24508196 MS.gene52335:CDS 40.0%
GGAGGATCATTATGACAAAC+AGG - chr7.3:24507522-24507541 MS.gene52335:CDS 40.0%
GGATGACAAAGCAAATAAGG+AGG - chr7.3:24508035-24508054 MS.gene52335:CDS 40.0%
! AAATGATGACGAGGATGAGA+AGG - chr7.3:24507855-24507874 MS.gene52335:CDS 40.0%
! GAAATCTGGTGATGTCACTA+TGG - chr7.3:24508155-24508174 MS.gene52335:CDS 40.0%
!! TGCTAGGATTAAGTTGAAGC+AGG - chr7.3:24507666-24507685 MS.gene52335:CDS 40.0%
!!! AGATTTTTCAAGAGACCCAC+GGG - chr7.3:24507630-24507649 MS.gene52335:CDS 40.0%
!!! GAGATTTTTCAAGAGACCCA+CGG - chr7.3:24507629-24507648 MS.gene52335:CDS 40.0%
AAACAGGCTCTTGGCAAAGA+GGG - chr7.3:24507538-24507557 MS.gene52335:CDS 45.0%
AGAAGTTGAACCGCAAGTAC+AGG - chr7.3:24508328-24508347 MS.gene52335:CDS 45.0%
ATCACAGGCAGATGAAGCTT+TGG - chr7.3:24508098-24508117 MS.gene52335:CDS 45.0%
CAGTGATGTATGTGCAATGG+AGG - chr7.3:24508263-24508282 MS.gene52335:CDS 45.0%
CTAGGCCCAATAAACGTGTT+CGG - chr7.3:24507812-24507831 MS.gene52335:CDS 45.0%
GGATGAACAGGAAGTTATCG+AGG - chr7.3:24508287-24508306 MS.gene52335:CDS 45.0%
GGGATAAGTTCACGTGTGTT+TGG - chr7.3:24507607-24507626 MS.gene52335:CDS 45.0%
GGGGGATCGTTTGTATATGA+GGG - chr7.3:24507564-24507583 MS.gene52335:CDS 45.0%
TCGAATGAAGACCTCCTCAA+TGG - chr7.3:24507894-24507913 MS.gene52335:CDS 45.0%
TGATGTATGTGCAATGGAGG+AGG - chr7.3:24508266-24508285 MS.gene52335:CDS 45.0%
TTATTGGGCCTAGACTCTGA+AGG + chr7.3:24507805-24507824 None:intergenic 45.0%
! CGGTTTTCTTCCTGTACTTG+CGG + chr7.3:24508341-24508360 None:intergenic 45.0%
AGCTGCATCCTCTTCCATTG+AGG + chr7.3:24507911-24507930 None:intergenic 50.0%
AGGATGAGAAGGAAGGAACC+AGG - chr7.3:24507866-24507885 MS.gene52335:CDS 50.0%
CAAACAGGCTCTTGGCAAAG+AGG - chr7.3:24507537-24507556 MS.gene52335:CDS 50.0%
CATCGGCAGCTATGCTAACA+GGG + chr7.3:24508230-24508249 None:intergenic 50.0%
GAAGAAAACCGACGAGGACT+TGG - chr7.3:24508350-24508369 MS.gene52335:CDS 50.0%
GAAGACCTCCTCAATGGAAG+AGG - chr7.3:24507900-24507919 MS.gene52335:CDS 50.0%
GAGAAACCTACACCAGACGT+CGG - chr7.3:24507730-24507749 MS.gene52335:CDS 50.0%
GATGACGAGGATGAGAAGGA+AGG - chr7.3:24507859-24507878 MS.gene52335:CDS 50.0%
GGATGAGAAGGAAGGAACCA+GGG - chr7.3:24507867-24507886 MS.gene52335:CDS 50.0%
GGGGGGATCGTTTGTATATG+AGG - chr7.3:24507563-24507582 MS.gene52335:CDS 50.0%
GGTCTTCATTCGAACATCCC+TGG + chr7.3:24507887-24507906 None:intergenic 50.0%
GTACAGGAAGAAAACCGACG+AGG - chr7.3:24508344-24508363 MS.gene52335:CDS 50.0%
TCATCGGCAGCTATGCTAAC+AGG + chr7.3:24508231-24508250 None:intergenic 50.0%
TGCAATGGAGGAGGATGAAC+AGG - chr7.3:24508275-24508294 MS.gene52335:CDS 50.0%
TGCATCCTCTTCCATTGAGG+AGG + chr7.3:24507908-24507927 None:intergenic 50.0%
AAACCTACACCAGACGTCGG+TGG - chr7.3:24507733-24507752 MS.gene52335:CDS 55.0%
GAAAACCGACGAGGACTTGG+AGG - chr7.3:24508353-24508372 MS.gene52335:CDS 55.0%
GGCAGCTATGCTAACAGGGA+AGG + chr7.3:24508226-24508245 None:intergenic 55.0%
GGGCTAATCATACCGCTGCT+AGG - chr7.3:24507650-24507669 MS.gene52335:CDS 55.0%
! CAGCGGTATGATTAGCCCGT+GGG + chr7.3:24507648-24507667 None:intergenic 55.0%
AGGCTCCTCCAAGTCCTCGT+CGG + chr7.3:24508361-24508380 None:intergenic 60.0%
AGGCTCTTGGCAAAGAGGGC+AGG - chr7.3:24507542-24507561 MS.gene52335:CDS 60.0%
CTCTTGGCAAAGAGGGCAGG+GGG - chr7.3:24507545-24507564 MS.gene52335:CDS 60.0%
GCTCTTGGCAAAGAGGGCAG+GGG - chr7.3:24507544-24507563 MS.gene52335:CDS 60.0%
GGCTCTTGGCAAAGAGGGCA+GGG - chr7.3:24507543-24507562 MS.gene52335:CDS 60.0%
TCTTGGCAAAGAGGGCAGGG+GGG - chr7.3:24507546-24507565 MS.gene52335:CDS 60.0%
! GCAGCGGTATGATTAGCCCG+TGG + chr7.3:24507649-24507668 None:intergenic 60.0%
CCACCACCGACGTCTGGTGT+AGG + chr7.3:24507739-24507758 None:intergenic 65.0%
CCTACACCAGACGTCGGTGG+TGG - chr7.3:24507736-24507755 MS.gene52335:CDS 65.0%
GCATCACCACCACCGACGTC+TGG + chr7.3:24507745-24507764 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 24507511 24508389 24507511 ID=MS.gene52335
chr7.3 mRNA 24507511 24508389 24507511 ID=MS.gene52335.t1;Parent=MS.gene52335
chr7.3 exon 24507511 24508389 24507511 ID=MS.gene52335.t1.exon1;Parent=MS.gene52335.t1
chr7.3 CDS 24507511 24508389 24507511 ID=cds.MS.gene52335.t1;Parent=MS.gene52335.t1
Gene Sequence

>MS.gene52335

ATGCAGGATGAGGAGGATCATTATGACAAACAGGCTCTTGGCAAAGAGGGCAGGGGGGATCGTTTGTATATGAGGGAGATAAATGAAGATGGAATCGGGATAAGTTCACGTGTGTTTGGAGATTTTTCAAGAGACCCACGGGCTAATCATACCGCTGCTAGGATTAAGTTGAAGCAGGATGCTGAAGCAGCCAATCAAATTGATACTTTGCATGTCTCTGAGAAACCTACACCAGACGTCGGTGGTGGTGATGCTGTTAACCCCAAGTCAATTTTGAAGAGTAAAGATAATCCTTCAGAGTCTAGGCCCAATAAACGTGTTCGGTTTGATTCTGATAAAGATGGAAATGATGACGAGGATGAGAAGGAAGGAACCAGGGATGTTCGAATGAAGACCTCCTCAATGGAAGAGGATGCAGCTTTAAATCAGCCATCAAAGTCACAGGAATTTGCTTCTGCAGTTCCTGATTATATTCGCAATCCTTCAAAATACACACATTATACTTTTGATTCTTCAACTGACATGGATGACAAAGCAAATAAGGAGGCATACATGAGTTTCCTTGCACAGATAAAAGGTTCAAATGCATCACAGGCAGATGAAGCTTTGGATGACCTTCCATCTGTTACCTTTATACCGAAGAAGAAATCTGGTGATGTCACTATGGGAGAGAATAAGCTTGAAGTTGGAAAGGAGTGCATGAATAAAAAAGCCTTCCCTGTTAGCATAGCTGCCGATGATGACACTGAAAACAGTGATGTATGTGCAATGGAGGAGGATGAACAGGAAGTTATCGAGGATACTAAAAAAAGCTCTCAGAAGTTGAACCGCAAGTACAGGAAGAAAACCGACGAGGACTTGGAGGAGCCTACTGTGTAG

Protein sequence

>MS.gene52335.t1

MQDEEDHYDKQALGKEGRGDRLYMREINEDGIGISSRVFGDFSRDPRANHTAARIKLKQDAEAANQIDTLHVSEKPTPDVGGGDAVNPKSILKSKDNPSESRPNKRVRFDSDKDGNDDEDEKEGTRDVRMKTSSMEEDAALNQPSKSQEFASAVPDYIRNPSKYTHYTFDSSTDMDDKANKEAYMSFLAQIKGSNASQADEALDDLPSVTFIPKKKSGDVTMGENKLEVGKECMNKKAFPVSIAADDDTENSDVCAMEEDEQEVIEDTKKSSQKLNRKYRKKTDEDLEEPTV