AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53249


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53249.t1 MTR_3g005710 98.535 273 4 0 1 273 26 298 0.0 568
MS.gene53249.t1 MTR_8g080660 45.000 240 118 5 47 272 58 297 1.90e-68 214
MS.gene53249.t1 MTR_3g085270 46.977 215 109 4 51 260 60 274 1.71e-66 209
MS.gene53249.t1 MTR_4g485570 42.791 215 119 2 52 262 68 282 2.47e-58 188
MS.gene53249.t1 MTR_4g485560 42.925 212 117 2 55 262 21 232 3.78e-56 181
MS.gene53249.t1 MTR_7g029100 45.588 204 94 3 59 261 79 266 2.42e-55 180
MS.gene53249.t1 MTR_4g094895 38.839 224 131 4 41 258 60 283 1.12e-50 169
MS.gene53249.t1 MTR_4g102900 36.134 238 142 6 42 273 64 297 7.04e-42 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53249.t1 AT4G09990 70.293 239 67 3 36 273 48 283 3.06e-128 366
MS.gene53249.t1 AT1G33800 67.886 246 77 2 28 273 47 290 7.27e-128 365
MS.gene53249.t1 AT1G09610 65.079 252 80 4 27 273 34 282 6.07e-122 349
MS.gene53249.t1 AT1G71690 58.519 270 94 6 9 273 39 295 1.15e-110 322
MS.gene53249.t1 AT1G67330 50.935 214 101 3 53 262 65 278 1.03e-73 227
MS.gene53249.t1 AT1G27930 49.528 212 104 2 53 261 62 273 8.15e-72 222
MS.gene53249.t1 AT5G67210 38.430 242 143 4 23 258 49 290 1.59e-52 174
MS.gene53249.t1 AT2G15440 37.054 224 131 5 58 272 81 303 1.13e-50 169
MS.gene53249.t1 AT3G50220 40.376 213 121 4 52 258 85 297 1.06e-49 167
MS.gene53249.t1 AT4G24910 34.536 194 120 4 74 260 104 297 2.07e-29 113

Find 68 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTGCAACTTCTTGGTCTT+TGG 0.164120 3.3:-1152302 MS.gene53249:CDS
AAAATCACCTTGCAACTTCT+TGG 0.221414 3.3:-1152309 MS.gene53249:CDS
AAATGTGGATTAGCACTTAA+AGG 0.223364 3.3:-1152059 MS.gene53249:CDS
AGTGATGAACTAATGAAAAT+TGG 0.261741 3.3:-1152128 MS.gene53249:CDS
AAATCAATTTCATGGACAAT+AGG 0.306327 3.3:+1151755 None:intergenic
ATTGCTAGGAATAGAGAAAA+TGG 0.307486 3.3:-1151924 MS.gene53249:CDS
TTTGTGCATGTTGGTGTTAT+AGG 0.311759 3.3:+1152437 None:intergenic
CCAACGTTGGGAACTTTGAT+TGG 0.316396 3.3:+1152189 None:intergenic
ACCAAGTCATAGGGCACGTT+TGG 0.318589 3.3:-1151784 MS.gene53249:CDS
TCCATGTCTTGTCTTCTTCC+AGG 0.323320 3.3:+1152222 None:intergenic
GGGTACTTCGATGGCGCGCC+GGG 0.332994 3.3:-1151978 MS.gene53249:CDS
TTGTGCATGTTGGTGTTATA+GGG 0.335274 3.3:+1152438 None:intergenic
AACTTCTTGGTCTTTGGATT+AGG 0.336773 3.3:-1152296 MS.gene53249:CDS
ACTTCAATATACCAAGTCAT+AGG 0.375565 3.3:-1151794 MS.gene53249:CDS
ATACAGCTGGTTTGATTGCT+AGG 0.379409 3.3:-1151938 MS.gene53249:CDS
AATCAATTTCATGGACAATA+GGG 0.382464 3.3:+1151756 None:intergenic
TGTTTGTTCATGATGTTGAT+AGG 0.396580 3.3:-1151890 MS.gene53249:CDS
TGGTTGAGAAAATGAGAAAA+TGG 0.434558 3.3:+1152547 None:intergenic
ATGAGTGCTATTTATACAGC+TGG 0.441985 3.3:-1151951 MS.gene53249:CDS
TTGGTGTTTGAAGTTGTTGT+GGG 0.443623 3.3:+1152524 None:intergenic
TCTCTTGAATTGTTTGTTGA+GGG 0.447760 3.3:+1152360 None:intergenic
CTTGTCTTCTTCCAGGAAAA+CGG 0.456850 3.3:+1152229 None:intergenic
TGCATAATGGATTAATGCAT+TGG 0.464703 3.3:+1152397 None:intergenic
TTCTTTGTGAAGGTTATTTG+AGG 0.465004 3.3:-1151839 MS.gene53249:CDS
CCAAGTCATAGGGCACGTTT+GGG 0.466887 3.3:-1151783 MS.gene53249:CDS
TCTTTGTGAAGGTTATTTGA+GGG 0.475343 3.3:-1151838 MS.gene53249:CDS
GGGGTACTTCGATGGCGCGC+CGG 0.481478 3.3:-1151979 MS.gene53249:CDS
CCCAAACGTGCCCTATGACT+TGG 0.483562 3.3:+1151783 None:intergenic
GTTGGTGTTTGAAGTTGTTG+TGG 0.491866 3.3:+1152523 None:intergenic
ATCATGGTGGATGCACCAAC+GGG 0.492498 3.3:-1151999 MS.gene53249:CDS
ATCTCTTGAATTGTTTGTTG+AGG 0.496296 3.3:+1152359 None:intergenic
CATGATATGATTCCAACGTT+GGG 0.499455 3.3:+1152177 None:intergenic
GGGAACAAGAAGGAAGGATT+AGG 0.499937 3.3:-1151818 MS.gene53249:CDS
TGAAATTTATGATGTGGAAT+GGG 0.503077 3.3:-1152027 MS.gene53249:CDS
TCCACATCATAAATTTCACT+TGG 0.503817 3.3:+1152032 None:intergenic
TCCAAGTGAAATTTATGATG+TGG 0.511674 3.3:-1152033 MS.gene53249:CDS
GTGAAATTTATGATGTGGAA+TGG 0.519138 3.3:-1152028 MS.gene53249:CDS
GCACCAACGGGGTACTTCGA+TGG 0.521410 3.3:-1151987 MS.gene53249:CDS
TGTGGAATGGGATGTGATCA+TGG 0.525871 3.3:-1152015 MS.gene53249:CDS
CATCATTGAACTACGGTGGC+CGG 0.528792 3.3:-1152253 MS.gene53249:CDS
ATTAATGCATTGGCTAATGA+TGG 0.530150 3.3:+1152407 None:intergenic
CTTCTTCCAGGAAAACGGTC+CGG 0.530964 3.3:+1152234 None:intergenic
ACATGATATGATTCCAACGT+TGG 0.536855 3.3:+1152176 None:intergenic
ATTTGAGGGAACAAGAAGGA+AGG 0.541340 3.3:-1151824 MS.gene53249:CDS
ACTTCGATGGCGCGCCGGGG+AGG 0.549464 3.3:-1151974 MS.gene53249:CDS
GTGTTTGAAGTTGTTGTGGG+TGG 0.550586 3.3:+1152527 None:intergenic
GAAAATTGGAATGGAACAAG+AGG 0.553174 3.3:-1152114 MS.gene53249:CDS
TCCTGGAAGAAGACAAGACA+TGG 0.567326 3.3:-1152223 MS.gene53249:CDS
GTTGGTGTTATAGGGAGTGA+AGG 0.571518 3.3:+1152446 None:intergenic
TGAACTAATGAAAATTGGAA+TGG 0.575241 3.3:-1152123 MS.gene53249:CDS
AATGCATTGGCTAATGATGG+AGG 0.575854 3.3:+1152410 None:intergenic
CCAATCAAAGTTCCCAACGT+TGG 0.578373 3.3:-1152189 MS.gene53249:CDS
CTCTTGAATTGTTTGTTGAG+GGG 0.579049 3.3:+1152361 None:intergenic
ATGCATTGGCTAATGATGGA+GGG 0.579142 3.3:+1152411 None:intergenic
CTTCAATATACCAAGTCATA+GGG 0.581013 3.3:-1151793 MS.gene53249:CDS
GGTTATTTGAGGGAACAAGA+AGG 0.586352 3.3:-1151828 MS.gene53249:CDS
TGATGTTGATAGGAAAGTTG+AGG 0.590781 3.3:-1151880 MS.gene53249:CDS
GATCATGGTGGATGCACCAA+CGG 0.605400 3.3:-1152000 MS.gene53249:CDS
TTGGTGTTATAGGGAGTGAA+GGG 0.609114 3.3:+1152447 None:intergenic
CCAAAGACCAAGAAGTTGCA+AGG 0.614426 3.3:+1152302 None:intergenic
GCGCCATCGAAGTACCCCGT+TGG 0.633771 3.3:+1151984 None:intergenic
TAAATAGCACTCATCCTCCC+CGG 0.635229 3.3:+1151960 None:intergenic
ATGTGGACATCATTGAACTA+CGG 0.637354 3.3:-1152260 MS.gene53249:CDS
TAGGTCATGATAGTCTCATG+TGG 0.666366 3.3:-1152277 MS.gene53249:CDS
TGGACATCATTGAACTACGG+TGG 0.678674 3.3:-1152257 MS.gene53249:CDS
GGTACTTCGATGGCGCGCCG+GGG 0.680331 3.3:-1151977 MS.gene53249:CDS
GGAATGGGATGTGATCATGG+TGG 0.725748 3.3:-1152012 MS.gene53249:CDS
TCATGGTGGATGCACCAACG+GGG 0.763267 3.3:-1151998 MS.gene53249:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGATTTTTTTTGAAGAACTT+TGG + chr3.3:1152005-1152024 None:intergenic 20.0%
! AGTGATGAACTAATGAAAAT+TGG - chr3.3:1152199-1152218 MS.gene53249:CDS 25.0%
! TGAAATTTATGATGTGGAAT+GGG - chr3.3:1152300-1152319 MS.gene53249:CDS 25.0%
! TGAACTAATGAAAATTGGAA+TGG - chr3.3:1152204-1152223 MS.gene53249:CDS 25.0%
!! TTTCTTGTGATGAGATTTTT+GGG + chr3.3:1151835-1151854 None:intergenic 25.0%
!!! AATCCACATTTTGAAAATCT+TGG + chr3.3:1152259-1152278 None:intergenic 25.0%
!!! ATCCACATTTTGAAAATCTT+GGG + chr3.3:1152258-1152277 None:intergenic 25.0%
!!! TGATTTTTGTTGTTGCATAA+TGG + chr3.3:1151946-1151965 None:intergenic 25.0%
ACTTCAATATACCAAGTCAT+AGG - chr3.3:1152533-1152552 MS.gene53249:CDS 30.0%
ATTGCTAGGAATAGAGAAAA+TGG - chr3.3:1152403-1152422 MS.gene53249:CDS 30.0%
CTTCAATATACCAAGTCATA+GGG - chr3.3:1152534-1152553 MS.gene53249:CDS 30.0%
GTGAAATTTATGATGTGGAA+TGG - chr3.3:1152299-1152318 MS.gene53249:CDS 30.0%
TCCAAGTGAAATTTATGATG+TGG - chr3.3:1152294-1152313 MS.gene53249:CDS 30.0%
TCCACATCATAAATTTCACT+TGG + chr3.3:1152298-1152317 None:intergenic 30.0%
TCTTTGTGAAGGTTATTTGA+GGG - chr3.3:1152489-1152508 MS.gene53249:CDS 30.0%
TGGTTGAGAAAATGAGAAAA+TGG + chr3.3:1151783-1151802 None:intergenic 30.0%
TGTTTGTTCATGATGTTGAT+AGG - chr3.3:1152437-1152456 MS.gene53249:CDS 30.0%
TTCTTTGTGAAGGTTATTTG+AGG - chr3.3:1152488-1152507 MS.gene53249:CDS 30.0%
! AGTTGAGGATCAATTTTCAA+AGG - chr3.3:1152462-1152481 MS.gene53249:CDS 30.0%
! ATCTCTTGAATTGTTTGTTG+AGG + chr3.3:1151971-1151990 None:intergenic 30.0%
! GTTTCTTGTGATGAGATTTT+TGG + chr3.3:1151836-1151855 None:intergenic 30.0%
! TCTCTTGAATTGTTTGTTGA+GGG + chr3.3:1151970-1151989 None:intergenic 30.0%
!! AAATGTGGATTAGCACTTAA+AGG - chr3.3:1152268-1152287 MS.gene53249:CDS 30.0%
!! ATTAATGCATTGGCTAATGA+TGG + chr3.3:1151923-1151942 None:intergenic 30.0%
!! TGCATAATGGATTAATGCAT+TGG + chr3.3:1151933-1151952 None:intergenic 30.0%
AAAATCACCTTGCAACTTCT+TGG - chr3.3:1152018-1152037 MS.gene53249:CDS 35.0%
ACATGATATGATTCCAACGT+TGG + chr3.3:1152154-1152173 None:intergenic 35.0%
ATGTGGACATCATTGAACTA+CGG - chr3.3:1152067-1152086 MS.gene53249:CDS 35.0%
CATGATATGATTCCAACGTT+GGG + chr3.3:1152153-1152172 None:intergenic 35.0%
GAAAATTGGAATGGAACAAG+AGG - chr3.3:1152213-1152232 MS.gene53249:CDS 35.0%
! AACTTCTTGGTCTTTGGATT+AGG - chr3.3:1152031-1152050 MS.gene53249:CDS 35.0%
! CTCTTGAATTGTTTGTTGAG+GGG + chr3.3:1151969-1151988 None:intergenic 35.0%
! GACCCAAGATTTTCAAAATG+TGG - chr3.3:1152253-1152272 MS.gene53249:CDS 35.0%
! TCAAAGGCTTTTCTTTGTGA+AGG - chr3.3:1152478-1152497 MS.gene53249:CDS 35.0%
! TGATGTTGATAGGAAAGTTG+AGG - chr3.3:1152447-1152466 MS.gene53249:CDS 35.0%
! TTGGTGTTTGAAGTTGTTGT+GGG + chr3.3:1151806-1151825 None:intergenic 35.0%
!! ATGAGTGCTATTTATACAGC+TGG - chr3.3:1152376-1152395 MS.gene53249:CDS 35.0%
!! TTGTGCATGTTGGTGTTATA+GGG + chr3.3:1151892-1151911 None:intergenic 35.0%
!! TTTGTGCATGTTGGTGTTAT+AGG + chr3.3:1151893-1151912 None:intergenic 35.0%
!!! TGAGATTTTTGGGTCATTGT+TGG + chr3.3:1151825-1151844 None:intergenic 35.0%
ATTTGAGGGAACAAGAAGGA+AGG - chr3.3:1152503-1152522 MS.gene53249:CDS 40.0%
CTTGTCTTCTTCCAGGAAAA+CGG + chr3.3:1152101-1152120 None:intergenic 40.0%
GGTTATTTGAGGGAACAAGA+AGG - chr3.3:1152499-1152518 MS.gene53249:CDS 40.0%
TAGGTCATGATAGTCTCATG+TGG - chr3.3:1152050-1152069 MS.gene53249:CDS 40.0%
! GTTGGTGTTTGAAGTTGTTG+TGG + chr3.3:1151807-1151826 None:intergenic 40.0%
!! AATGCATTGGCTAATGATGG+AGG + chr3.3:1151920-1151939 None:intergenic 40.0%
!! ATACAGCTGGTTTGATTGCT+AGG - chr3.3:1152389-1152408 MS.gene53249:CDS 40.0%
!! ATGCATTGGCTAATGATGGA+GGG + chr3.3:1151919-1151938 None:intergenic 40.0%
!! TTGGTGTTATAGGGAGTGAA+GGG + chr3.3:1151883-1151902 None:intergenic 40.0%
CCAAAGACCAAGAAGTTGCA+AGG + chr3.3:1152028-1152047 None:intergenic 45.0%
CCAACGTTGGGAACTTTGAT+TGG + chr3.3:1152141-1152160 None:intergenic 45.0%
CCAATCAAAGTTCCCAACGT+TGG - chr3.3:1152138-1152157 MS.gene53249:CDS 45.0%
GGGAACAAGAAGGAAGGATT+AGG - chr3.3:1152509-1152528 MS.gene53249:CDS 45.0%
TAAATAGCACTCATCCTCCC+CGG + chr3.3:1152370-1152389 None:intergenic 45.0%
TCCATGTCTTGTCTTCTTCC+AGG + chr3.3:1152108-1152127 None:intergenic 45.0%
TCCTGGAAGAAGACAAGACA+TGG - chr3.3:1152104-1152123 MS.gene53249:CDS 45.0%
TGGACATCATTGAACTACGG+TGG - chr3.3:1152070-1152089 MS.gene53249:CDS 45.0%
TGTGGAATGGGATGTGATCA+TGG - chr3.3:1152312-1152331 MS.gene53249:CDS 45.0%
! CCTTGCAACTTCTTGGTCTT+TGG - chr3.3:1152025-1152044 MS.gene53249:CDS 45.0%
! GTGTTTGAAGTTGTTGTGGG+TGG + chr3.3:1151803-1151822 None:intergenic 45.0%
!! GTTGGTGTTATAGGGAGTGA+AGG + chr3.3:1151884-1151903 None:intergenic 45.0%
!!! GGAGGGTTTTTTGTGCATGT+TGG + chr3.3:1151902-1151921 None:intergenic 45.0%
ACCAAGTCATAGGGCACGTT+TGG - chr3.3:1152543-1152562 MS.gene53249:CDS 50.0%
CATCATTGAACTACGGTGGC+CGG - chr3.3:1152074-1152093 MS.gene53249:CDS 50.0%
CCAAGTCATAGGGCACGTTT+GGG - chr3.3:1152544-1152563 MS.gene53249:CDS 50.0%
CTTCTTCCAGGAAAACGGTC+CGG + chr3.3:1152096-1152115 None:intergenic 50.0%
GGAATGGGATGTGATCATGG+TGG - chr3.3:1152315-1152334 MS.gene53249:CDS 50.0%
!! ATCATGGTGGATGCACCAAC+GGG - chr3.3:1152328-1152347 MS.gene53249:CDS 50.0%
!! GATCATGGTGGATGCACCAA+CGG - chr3.3:1152327-1152346 MS.gene53249:CDS 50.0%
CCCAAACGTGCCCTATGACT+TGG + chr3.3:1152547-1152566 None:intergenic 55.0%
!! TCATGGTGGATGCACCAACG+GGG - chr3.3:1152329-1152348 MS.gene53249:CDS 55.0%
! GCACCAACGGGGTACTTCGA+TGG - chr3.3:1152340-1152359 MS.gene53249:CDS 60.0%
! GCGCCATCGAAGTACCCCGT+TGG + chr3.3:1152346-1152365 None:intergenic 65.0%
GGGTACTTCGATGGCGCGCC+GGG - chr3.3:1152349-1152368 MS.gene53249:CDS 70.0%
GGTACTTCGATGGCGCGCCG+GGG - chr3.3:1152350-1152369 MS.gene53249:CDS 70.0%
! CGGTGGCCGGACCGTTTTCC+TGG - chr3.3:1152087-1152106 MS.gene53249:CDS 70.0%
! GGGGTACTTCGATGGCGCGC+CGG - chr3.3:1152348-1152367 MS.gene53249:CDS 70.0%
ACTTCGATGGCGCGCCGGGG+AGG - chr3.3:1152353-1152372 MS.gene53249:CDS 75.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 1151764 1152585 1151764 ID=MS.gene53249
chr3.3 mRNA 1151764 1152585 1151764 ID=MS.gene53249.t1;Parent=MS.gene53249
chr3.3 exon 1151764 1152585 1151764 ID=MS.gene53249.t1.exon1;Parent=MS.gene53249.t1
chr3.3 CDS 1151764 1152585 1151764 ID=cds.MS.gene53249.t1;Parent=MS.gene53249.t1
Gene Sequence

>MS.gene53249

ATGCTATTCAAATCATCCATTTTCTCATTTTCTCAACCACCCACAACAACTTCAAACACCAACAATGACCCAAAAATCTCATCACAAGAAACAGAAAAAATCACAAAATCTGAATGCCCTTCACTCCCTATAACACCAACATGCACAAAAAACCCTCCATCATTAGCCAATGCATTAATCCATTATGCAACAACAAAAATCACCCCTCAACAAACAATTCAAGAGATCTCAGTCTCAGCCAAAGTTCTTCAAAAAAAATCACCTTGCAACTTCTTGGTCTTTGGATTAGGTCATGATAGTCTCATGTGGACATCATTGAACTACGGTGGCCGGACCGTTTTCCTGGAAGAAGACAAGACATGGATTGAGCAAATCCAATCAAAGTTCCCAACGTTGGAATCATATCATGTTGTGTATGATACAAAAGTACATCAAAGTGATGAACTAATGAAAATTGGAATGGAACAAGAGGATTGTAAAAAAGTTTCTGACCCAAGATTTTCAAAATGTGGATTAGCACTTAAAGGATTTCCAAGTGAAATTTATGATGTGGAATGGGATGTGATCATGGTGGATGCACCAACGGGGTACTTCGATGGCGCGCCGGGGAGGATGAGTGCTATTTATACAGCTGGTTTGATTGCTAGGAATAGAGAAAATGGTGATACTGATGTGTTTGTTCATGATGTTGATAGGAAAGTTGAGGATCAATTTTCAAAGGCTTTTCTTTGTGAAGGTTATTTGAGGGAACAAGAAGGAAGGATTAGGCACTTCAATATACCAAGTCATAGGGCACGTTTGGGTAGACCCTATTGTCCATGA

Protein sequence

>MS.gene53249.t1

MLFKSSIFSFSQPPTTTSNTNNDPKISSQETEKITKSECPSLPITPTCTKNPPSLANALIHYATTKITPQQTIQEISVSAKVLQKKSPCNFLVFGLGHDSLMWTSLNYGGRTVFLEEDKTWIEQIQSKFPTLESYHVVYDTKVHQSDELMKIGMEQEDCKKVSDPRFSKCGLALKGFPSEIYDVEWDVIMVDAPTGYFDGAPGRMSAIYTAGLIARNRENGDTDVFVHDVDRKVEDQFSKAFLCEGYLREQEGRIRHFNIPSHRARLGRPYCP