AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54235

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54235.t1 MTR_5g062230 70.000 420 126 0 1 420 37 456 0.0 615
MS.gene54235.t1 MTR_6g039440 69.524 420 128 0 1 420 37 456 0.0 613
MS.gene54235.t1 MTR_6g008560 68.171 421 133 1 1 420 40 460 0.0 608
MS.gene54235.t1 MTR_3g464190 66.033 421 142 1 1 420 40 460 0.0 589
MS.gene54235.t1 MTR_4g048460 65.952 420 143 0 1 420 45 464 0.0 580
MS.gene54235.t1 MTR_4g048460 65.952 420 143 0 1 420 45 464 0.0 579
MS.gene54235.t1 MTR_5g073260 65.877 422 142 2 1 420 34 455 0.0 570
MS.gene54235.t1 MTR_5g073200 66.746 421 139 1 1 420 32 452 0.0 569
MS.gene54235.t1 MTR_5g094620 63.095 420 136 1 1 420 40 440 0.0 558
MS.gene54235.t1 MTR_4g081460 59.952 417 165 1 6 420 49 465 0.0 531
MS.gene54235.t1 MTR_6g008560 66.776 304 101 0 117 420 1 304 3.66e-152 437
MS.gene54235.t1 MTR_8g007515 45.933 418 221 3 5 418 41 457 1.58e-132 392
MS.gene54235.t1 MTR_2g082010 43.499 423 235 4 1 420 50 471 2.10e-128 382
MS.gene54235.t1 MTR_2g081980 44.630 419 226 6 5 418 41 458 5.91e-128 381
MS.gene54235.t1 MTR_2g082060 43.499 423 233 5 1 418 44 465 3.16e-127 379
MS.gene54235.t1 MTR_4g019550 41.135 423 228 5 5 418 49 459 2.03e-108 330
MS.gene54235.t1 MTR_4g045810 37.653 409 251 2 12 419 78 483 7.70e-96 299
MS.gene54235.t1 MTR_2g064425 40.146 411 236 6 9 416 83 486 6.44e-94 294
MS.gene54235.t1 MTR_2g082050 38.084 407 195 6 14 418 9 360 1.24e-93 289
MS.gene54235.t1 MTR_2g089130 35.835 413 251 3 10 419 67 468 1.37e-87 277
MS.gene54235.t1 MTR_2g089120 35.507 414 252 4 10 419 67 469 1.38e-86 274
MS.gene54235.t1 MTR_8g057020 38.933 375 223 3 12 384 87 457 1.94e-86 275
MS.gene54235.t1 MTR_2g064425 37.956 411 230 7 9 416 83 471 1.36e-83 267
MS.gene54235.t1 MTR_2g064425 43.807 331 177 5 9 337 83 406 1.68e-83 263
MS.gene54235.t1 MTR_2g065450 36.609 407 249 5 13 416 90 490 4.46e-82 263
MS.gene54235.t1 MTR_5g094530 56.164 219 58 4 127 342 1 184 4.78e-76 235
MS.gene54235.t1 MTR_2g064425 41.088 331 171 6 9 337 83 391 2.37e-73 236
MS.gene54235.t1 MTR_4g092020 33.333 417 270 4 5 420 64 473 8.73e-72 236
MS.gene54235.t1 MTR_4g092010 31.931 404 269 4 16 416 61 461 6.60e-69 228
MS.gene54235.t1 MTR_7g010710 32.487 394 256 6 6 394 44 432 5.12e-61 207
MS.gene54235.t1 MTR_7g050990 60.606 165 54 2 161 325 28 181 9.86e-59 206
MS.gene54235.t1 MTR_2g010960 28.873 426 286 5 3 416 2 422 1.01e-58 200
MS.gene54235.t1 MTR_6g064980 29.512 410 277 7 15 416 13 418 3.09e-55 191
MS.gene54235.t1 MTR_3g465090 29.657 408 279 6 15 416 13 418 9.20e-55 189
MS.gene54235.t1 MTR_3g063170 29.024 410 279 7 15 416 13 418 1.22e-53 187
MS.gene54235.t1 MTR_3g063120 28.718 390 270 6 34 416 6 394 3.74e-52 182
MS.gene54235.t1 MTR_3g063140 28.537 410 281 7 15 416 13 418 1.46e-50 178
MS.gene54235.t1 MTR_3g052120 29.167 408 281 7 15 416 13 418 1.93e-50 178
MS.gene54235.t1 MTR_6g065040 28.931 318 216 5 106 416 2 316 1.44e-41 152
MS.gene54235.t1 MTR_3g063160 31.295 278 186 5 15 289 13 288 9.33e-36 133
MS.gene54235.t1 MTR_3g058160 27.692 390 248 10 30 392 64 446 1.03e-34 135
MS.gene54235.t1 MTR_3g058160 27.692 390 248 10 30 392 255 637 1.71e-34 136
MS.gene54235.t1 MTR_3g058160 27.692 390 248 10 30 392 268 650 2.07e-34 136
MS.gene54235.t1 MTR_3g058160 27.692 390 248 10 30 392 275 657 2.43e-34 135
MS.gene54235.t1 MTR_2g064295 27.717 368 232 10 30 370 255 615 8.36e-34 134
MS.gene54235.t1 MTR_3g058160 26.764 411 246 11 30 392 275 678 8.01e-32 128
MS.gene54235.t1 MTR_7g057390 66.667 75 25 0 346 420 1 75 6.42e-29 111
MS.gene54235.t1 MTR_7g057340 66.667 75 25 0 346 420 1 75 2.72e-28 110
MS.gene54235.t1 MTR_2g012870 24.390 410 281 12 31 418 254 656 1.86e-26 112
MS.gene54235.t1 MTR_2g012900 24.824 427 276 16 29 418 252 670 3.37e-24 105
MS.gene54235.t1 MTR_7g011663 30.112 269 156 6 34 271 349 616 1.92e-23 103
MS.gene54235.t1 MTR_7g094970 27.239 268 167 6 34 274 211 477 2.00e-23 103
MS.gene54235.t1 MTR_5g030050 28.030 264 157 7 34 273 3 257 1.11e-20 92.4
MS.gene54235.t1 MTR_7g011770 28.472 288 177 7 34 293 245 531 1.17e-20 95.1
MS.gene54235.t1 MTR_3g015080 45.333 75 20 1 346 420 1 54 9.26e-13 65.5
MS.gene54235.t1 MTR_5g081730 35.455 110 66 1 114 218 2 111 5.48e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54235.t1 AT5G23960 38.049 410 251 2 13 420 41 449 8.73e-107 326
MS.gene54235.t1 AT5G23960 38.015 413 249 4 13 420 41 451 1.46e-105 323
MS.gene54235.t1 AT1G70080 37.890 417 251 5 9 418 96 511 2.97e-103 319
MS.gene54235.t1 AT1G31950 37.063 429 255 4 4 420 84 509 1.03e-98 305
MS.gene54235.t1 AT1G31950 37.063 429 255 4 4 420 84 509 7.48e-98 305
MS.gene54235.t1 AT3G14540 37.981 416 252 2 9 420 90 503 1.41e-97 301
MS.gene54235.t1 AT1G31950 37.176 425 252 4 8 420 87 508 5.45e-97 303
MS.gene54235.t1 AT3G14520 37.170 417 254 3 9 420 93 506 1.17e-96 301
MS.gene54235.t1 AT3G14540 37.981 416 252 2 9 420 90 503 1.32e-96 301
MS.gene54235.t1 AT3G14490 36.804 413 252 4 13 420 95 503 2.28e-93 293
MS.gene54235.t1 AT5G44630 34.988 423 259 5 9 420 43 460 3.36e-89 281
MS.gene54235.t1 AT3G29410 36.585 410 256 3 13 420 99 506 3.51e-89 282
MS.gene54235.t1 AT4G15870 35.446 426 252 7 3 420 90 500 4.32e-89 282
MS.gene54235.t1 AT3G29410 36.990 392 243 3 31 420 12 401 1.18e-88 278
MS.gene54235.t1 AT4G20200 35.862 435 246 10 8 420 84 507 6.26e-88 279
MS.gene54235.t1 AT4G20210 34.033 429 264 7 7 420 79 503 7.16e-87 276
MS.gene54235.t1 AT3G29190 34.917 421 258 7 6 416 86 500 2.17e-86 275
MS.gene54235.t1 AT1G33750 34.928 418 267 3 5 420 92 506 4.55e-86 274
MS.gene54235.t1 AT3G32030 34.048 420 271 4 5 420 89 506 8.73e-86 273
MS.gene54235.t1 AT4G13300 34.442 421 267 5 6 420 39 456 3.31e-84 268
MS.gene54235.t1 AT4G20230 33.571 420 267 6 9 420 92 507 4.31e-83 266
MS.gene54235.t1 AT4G20230 33.571 420 267 6 9 420 104 519 4.41e-83 267
MS.gene54235.t1 AT4G16730 33.652 419 259 7 13 419 27 438 2.34e-79 255
MS.gene54235.t1 AT4G13280 33.971 418 267 5 6 417 39 453 3.45e-79 255
MS.gene54235.t1 AT2G23230 34.434 424 264 8 3 416 79 498 9.59e-79 255
MS.gene54235.t1 AT3G29110 35.238 420 260 7 9 420 85 500 2.38e-78 254
MS.gene54235.t1 AT1G66020 33.493 418 270 5 9 420 86 501 1.32e-77 252
MS.gene54235.t1 AT4G16730 33.920 398 245 6 34 419 1 392 1.38e-77 249
MS.gene54235.t1 AT2G23230 34.198 424 260 9 3 416 79 493 2.93e-76 248
MS.gene54235.t1 AT4G16740 34.689 418 251 9 13 419 60 466 1.59e-74 243
MS.gene54235.t1 AT2G24210 33.652 419 261 8 11 419 78 489 1.65e-71 236
MS.gene54235.t1 AT1G48800 32.381 420 269 8 9 418 93 507 6.82e-70 232
MS.gene54235.t1 AT1G61680 32.117 411 268 3 15 420 77 481 1.39e-69 230
MS.gene54235.t1 AT4G13280 32.536 418 243 6 6 417 39 423 8.50e-69 227
MS.gene54235.t1 AT3G25810 31.785 409 261 7 23 419 92 494 4.10e-67 224
MS.gene54235.t1 AT1G66020 35.799 338 209 5 9 340 86 421 5.75e-67 219
MS.gene54235.t1 AT4G16740 36.765 340 197 6 13 342 60 391 1.89e-66 217
MS.gene54235.t1 AT3G25830 31.579 418 273 8 11 419 79 492 3.15e-66 222
MS.gene54235.t1 AT3G25820 31.579 418 273 8 11 419 79 492 3.15e-66 222
MS.gene54235.t1 AT1G61680 31.630 411 263 4 15 420 77 474 4.87e-66 221
MS.gene54235.t1 AT5G48110 40.574 244 142 3 176 418 1 242 6.96e-65 211
MS.gene54235.t1 AT1G48820 30.935 417 240 9 9 420 93 466 1.34e-62 211
MS.gene54235.t1 AT3G25820 33.046 348 221 7 11 350 79 422 3.43e-59 199
MS.gene54235.t1 AT4G16740 37.370 289 164 5 13 291 60 341 8.23e-56 189
MS.gene54235.t1 AT4G16740 37.370 289 164 5 13 291 60 341 8.23e-56 189
MS.gene54235.t1 AT3G25820 34.028 288 180 5 11 291 79 363 7.24e-52 179
MS.gene54235.t1 AT3G25820 34.028 288 180 5 11 291 79 363 7.24e-52 179
MS.gene54235.t1 AT1G79460 27.949 390 230 15 30 383 273 647 2.01e-28 118
MS.gene54235.t1 AT1G61120 24.731 372 248 8 29 379 286 646 1.59e-23 103
MS.gene54235.t1 AT4G02780 29.020 255 153 5 34 261 334 587 1.13e-22 101

Find 69 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCTCTTGCTGTGCTATTT+AGG 0.103456 6.2:-70190834 MS.gene54235:CDS
TCTACTTGCATTGGCAAAAT+TGG 0.119753 6.2:-70189709 MS.gene54235:CDS
TTCTCTTGCTGTGCTATTTA+GGG 0.189824 6.2:-70190833 MS.gene54235:CDS
GGTTCATGGTGAAAATATTT+TGG 0.219650 6.2:-70189907 MS.gene54235:CDS
TTTCCTTGCTTGAGAATATT+GGG 0.241792 6.2:+70189424 None:intergenic
ATGCAAGTAGAATTTCATTA+TGG 0.248221 6.2:+70189721 None:intergenic
AGAAGAAATTAGTCAAGTTT+TGG 0.262222 6.2:-70190914 MS.gene54235:CDS
TACAAGAGTTATGTTCAAAA+TGG 0.269625 6.2:-70190883 MS.gene54235:CDS
GTCATCAATGTATGATAAAA+TGG 0.310334 6.2:+70189382 None:intergenic
TAATTGCTTGGAGGATCTTC+CGG 0.335446 6.2:-70189018 MS.gene54235:CDS
CTGCTATTCATTGCAGACTT+AGG 0.335573 6.2:-70187945 MS.gene54235:CDS
TTCCTTGCTCGTCCTTGAAT+TGG 0.345782 6.2:+70189997 None:intergenic
GAGTAGTAGAATGTTGCTTT+TGG 0.347068 6.2:-70189469 MS.gene54235:CDS
TGTGCCGCAATATTCATATC+AGG 0.348479 6.2:+70191054 None:intergenic
TGCAAGTAGAATTTCATTAT+GGG 0.387262 6.2:+70189722 None:intergenic
CAACAAAAGACATCTTTGAT+TGG 0.390973 6.2:-70187999 MS.gene54235:CDS
TTGCAAGAGAAGGACTCAAT+TGG 0.390998 6.2:+70189838 None:intergenic
GATTGACTCGATATGTCGTT+TGG 0.393872 6.2:-70190956 MS.gene54235:CDS
TAAAGCAACTTCGTGTAATC+CGG 0.404734 6.2:+70188999 None:intergenic
GTTGCTTTATAAATCACTCT+TGG 0.404999 6.2:-70188985 MS.gene54235:CDS
GGATGAATCCTGCTCATAGA+TGG 0.414619 6.2:+70189743 None:intergenic
TGCTATTCATTGCAGACTTA+GGG 0.421274 6.2:-70187944 MS.gene54235:CDS
TGCATGTACTCCGCTGTTGT+TGG 0.426336 6.2:+70188090 None:intergenic
AACAAAAGACATCTTTGATT+GGG 0.428042 6.2:-70187998 MS.gene54235:CDS
TAGGTGGGATATTAATTGCT+TGG 0.452782 6.2:-70189030 MS.gene54235:CDS
TACGACTTCTTACATTGGAA+TGG 0.454510 6.2:-70188031 MS.gene54235:CDS
TAATGAAATTCTACTTGCAT+TGG 0.460272 6.2:-70189718 MS.gene54235:CDS
ACGACTTCTTACATTGGAAT+GGG 0.460983 6.2:-70188030 MS.gene54235:CDS
TTCTGGTCACCTTCAGTCCA+TGG 0.466911 6.2:-70189865 MS.gene54235:CDS
GATGACACATATGATGCATA+TGG 0.467097 6.2:-70189365 MS.gene54235:CDS
GAGCAAGAAATGAAGAAAGA+AGG 0.470516 6.2:-70188945 MS.gene54235:CDS
TTACATTTCCATCTATGAGC+AGG 0.472894 6.2:-70189751 MS.gene54235:CDS
ATTGACTCGATATGTCGTTT+GGG 0.487347 6.2:-70190955 MS.gene54235:CDS
ATAGTTAGCATTCAACCATC+TGG 0.489896 6.2:+70188116 None:intergenic
AGGACTCAATTGGGTGGCCA+TGG 0.492991 6.2:+70189848 None:intergenic
ATATTGGGGCTCAAAATATA+CGG 0.495144 6.2:+70189439 None:intergenic
TGCAAGAGAAGGACTCAATT+GGG 0.499978 6.2:+70189839 None:intergenic
ATATAGGTGGTGGAAGGAGT+TGG 0.505587 6.2:-70189525 MS.gene54235:intron
CATGGGCTTTATGTTTCACC+TGG 0.509798 6.2:-70190799 MS.gene54235:intron
AGACGTGTTCAACCAATTCA+AGG 0.513816 6.2:-70190009 MS.gene54235:CDS
TAACTTCTTGCCACTAGCCT+AGG 0.521919 6.2:+70189771 None:intergenic
AGGAAATTTCAGTGAAACAT+TGG 0.522145 6.2:-70189979 MS.gene54235:CDS
GCCTCACATATGATGGTTCA+TGG 0.522278 6.2:-70189921 MS.gene54235:CDS
TAAGAAGTCGTAGCCAACAC+TGG 0.522936 6.2:+70188042 None:intergenic
ATGATGTGCTAACCTTTCAA+TGG 0.523871 6.2:+70189310 None:intergenic
GTGGGATATTAATTGCTTGG+AGG 0.529634 6.2:-70189027 MS.gene54235:CDS
TGGCCTCACTCATATAAGCT+TGG 0.531321 6.2:+70188136 None:intergenic
TTCCTTGCTTGAGAATATTG+GGG 0.534381 6.2:+70189425 None:intergenic
AACATTGGTCGTAGATGTTG+AGG 0.541350 6.2:-70189964 MS.gene54235:CDS
TTTAGGGTGTTGAGACAACA+TGG 0.541649 6.2:-70190817 MS.gene54235:CDS
AGCCCCAATATTCTCAAGCA+AGG 0.547455 6.2:-70189427 MS.gene54235:CDS
CTTATATGAGTGAGGCCAGA+TGG 0.552296 6.2:-70188131 MS.gene54235:CDS
CTTTGTATATAGGTGGTGGA+AGG 0.567617 6.2:-70189531 MS.gene54235:intron
GTATGAAGCCTCACATATGA+TGG 0.567934 6.2:-70189928 MS.gene54235:CDS
ATCAAGTGGTTACCCAGTGT+TGG 0.570889 6.2:-70188055 MS.gene54235:CDS
TTGGCTACGACTTCTTACAT+TGG 0.586299 6.2:-70188036 MS.gene54235:CDS
ACTACTCTATCTCGTGCATA+CGG 0.590301 6.2:+70189485 None:intergenic
CACATATCAACAATATCAAG+TGG 0.594145 6.2:-70188069 MS.gene54235:CDS
AAGAAGTCGTAGCCAACACT+GGG 0.613680 6.2:+70188043 None:intergenic
AACCAATTCAAGGACGAGCA+AGG 0.615624 6.2:-70189999 MS.gene54235:CDS
ATTGGGTGGCCATGGACTGA+AGG 0.620747 6.2:+70189856 None:intergenic
GGAACCTGATATGAATATTG+CGG 0.628405 6.2:-70191058 MS.gene54235:CDS
ACATTGGTCGTAGATGTTGA+GGG 0.633793 6.2:-70189963 MS.gene54235:CDS
ACCATGAACCATCATATGTG+AGG 0.634158 6.2:+70189920 None:intergenic
AATCCAAGCTTATATGAGTG+AGG 0.644710 6.2:-70188139 MS.gene54235:CDS
TTAGGGTGTTGAGACAACAT+GGG 0.650604 6.2:-70190816 MS.gene54235:CDS
GGATGAAGTTGTGTCTAGCG+AGG 0.679610 6.2:-70187923 None:intergenic
AAGAGAAGGACTCAATTGGG+TGG 0.688659 6.2:+70189842 None:intergenic
TAACTATACACCAACAACAG+CGG 0.710034 6.2:-70188100 MS.gene54235:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTCTTTATAGTTTAAT+CGG - chr6.2:70190697-70190716 MS.gene54235:intron 10.0%
!! ATTTATAATTTATTCAACAA+TGG - chr6.2:70190164-70190183 MS.gene54235:intron 10.0%
!!! AAATATTTTTACATCATTTT+AGG - chr6.2:70190295-70190314 MS.gene54235:intron 10.0%
!!! AGATAATAATTTTTTTTTTG+TGG + chr6.2:70188675-70188694 None:intergenic 10.0%
!!! ATTTCATTTTAAATTTTGTA+TGG - chr6.2:70190194-70190213 MS.gene54235:intron 10.0%
!!! CATTTTTTATGTTTTTAATT+TGG - chr6.2:70188269-70188288 MS.gene54235:intron 10.0%
!!! TCATAATAATATATTTTTCT+TGG + chr6.2:70190356-70190375 None:intergenic 10.0%
!! AATATTAATGTTATACATGT+AGG - chr6.2:70188747-70188766 MS.gene54235:intron 15.0%
!! ACAAATTGATAAACTAAAAA+AGG - chr6.2:70187947-70187966 MS.gene54235:CDS 15.0%
!! ATAAATTAATATGTCTCTAA+TGG - chr6.2:70188912-70188931 MS.gene54235:CDS 15.0%
!! CTATCAAAGAATAAAAATTA+AGG - chr6.2:70190568-70190587 MS.gene54235:intron 15.0%
!! GATAATAATTAAAAGAAATG+AGG + chr6.2:70188561-70188580 None:intergenic 15.0%
!! GATTAAAGATAAATTTCATA+TGG + chr6.2:70189750-70189769 None:intergenic 15.0%
!! TAATATGAATGTCTAAAAAA+TGG - chr6.2:70188404-70188423 MS.gene54235:intron 15.0%
!! TAATATTTGACTATATTCAT+GGG - chr6.2:70188375-70188394 MS.gene54235:intron 15.0%
!! TATAAATAAGAAACTAATAC+AGG + chr6.2:70188891-70188910 None:intergenic 15.0%
!! TCATAAAATTAAGATTACAA+AGG + chr6.2:70190674-70190693 None:intergenic 15.0%
!! TTAACATCATTTAATTACAT+AGG - chr6.2:70190757-70190776 MS.gene54235:intron 15.0%
!! TTATTATGAAGTTATTTGAT+TGG - chr6.2:70190367-70190386 MS.gene54235:intron 15.0%
!!! CTTATTCACATTTTTTTTTA+AGG - chr6.2:70188219-70188238 MS.gene54235:intron 15.0%
!!! TAATAATTTTTTTTTTGTGG+TGG + chr6.2:70188672-70188691 None:intergenic 15.0%
!!! TATTTTTTGTCTTTGTATAT+AGG - chr6.2:70189440-70189459 MS.gene54235:CDS 15.0%
!!! TTTAATTACATAGGTTTTTT+TGG - chr6.2:70190766-70190785 MS.gene54235:intron 15.0%
!! AATTGATAAACTAAAAAAGG+AGG - chr6.2:70187950-70187969 MS.gene54235:CDS 20.0%
!! ACAAAGACAAAAAATAGTAT+AGG + chr6.2:70189436-70189455 None:intergenic 20.0%
!! ATGGTTGTTTAATATTATTG+TGG - chr6.2:70188931-70188950 MS.gene54235:CDS 20.0%
!! GAAAAAGTTTCTTAATAAGA+TGG + chr6.2:70188815-70188834 None:intergenic 20.0%
!! GTAATATTTGACTATATTCA+TGG - chr6.2:70188374-70188393 MS.gene54235:intron 20.0%
!!! AATCAAAATGATTTTTAGGT+GGG - chr6.2:70189936-70189955 MS.gene54235:CDS 20.0%
!!! AATCCTTTTTTTACTAAAAG+TGG + chr6.2:70188434-70188453 None:intergenic 20.0%
!!! AATTTTGTATGGTTATCAAA+TGG - chr6.2:70190205-70190224 MS.gene54235:intron 20.0%
!!! ATTTTGTTTTAAATTAGGCA+GGG + chr6.2:70189368-70189387 None:intergenic 20.0%
!!! TATTTTGTTTTAAATTAGGC+AGG + chr6.2:70189369-70189388 None:intergenic 20.0%
!!! TGAATTAGAACTTTTTACAA+AGG - chr6.2:70189648-70189667 MS.gene54235:intron 20.0%
!!! TTGCAATCAAAATGATTTTT+AGG - chr6.2:70189932-70189951 MS.gene54235:CDS 20.0%
! AACAAAAGACATCTTTGATT+GGG - chr6.2:70190983-70191002 MS.gene54235:CDS 25.0%
! AAGAAAAATGTTTCATCATG+AGG - chr6.2:70190510-70190529 MS.gene54235:intron 25.0%
! AGGTAAAAACTTATTATCCA+TGG - chr6.2:70190588-70190607 MS.gene54235:intron 25.0%
! ATGCAAGTAGAATTTCATTA+TGG + chr6.2:70189263-70189282 None:intergenic 25.0%
! CATAAATCATAAAATATGCG+CGG - chr6.2:70188624-70188643 MS.gene54235:intron 25.0%
! GTCATCAATGTATGATAAAA+TGG + chr6.2:70189602-70189621 None:intergenic 25.0%
! TAAGTTTAACTCAATTGGTT+TGG - chr6.2:70188595-70188614 MS.gene54235:intron 25.0%
! TAATGAAATTCTACTTGCAT+TGG - chr6.2:70189263-70189282 MS.gene54235:intron 25.0%
! TACAAGAGTTATGTTCAAAA+TGG - chr6.2:70188098-70188117 MS.gene54235:CDS 25.0%
! TATCCATTGTTATATGAACT+TGG - chr6.2:70190448-70190467 MS.gene54235:intron 25.0%
! TGCAAGTAGAATTTCATTAT+GGG + chr6.2:70189262-70189281 None:intergenic 25.0%
! TTTCTTTATAGTTTAATCGG+TGG - chr6.2:70190700-70190719 MS.gene54235:intron 25.0%
!! AGAAGAAATTAGTCAAGTTT+TGG - chr6.2:70188067-70188086 MS.gene54235:CDS 25.0%
!! TAAGTTGAGATATATATACC+AGG + chr6.2:70188203-70188222 None:intergenic 25.0%
!! TTCCAAATTCTTTTTGATGT+AGG + chr6.2:70189317-70189336 None:intergenic 25.0%
!! TTTTTGTCTTTGTATATAGG+TGG - chr6.2:70189443-70189462 MS.gene54235:CDS 25.0%
!!! AACTCTTACTTCTTTTAAGA+AGG + chr6.2:70190082-70190101 None:intergenic 25.0%
!!! ACTCTTACTTCTTTTAAGAA+GGG + chr6.2:70190081-70190100 None:intergenic 25.0%
!!! AGACCACTTTTAGTAAAAAA+AGG - chr6.2:70188428-70188447 MS.gene54235:intron 25.0%
!!! ATGGTTTTAACTCTAAAGTT+TGG + chr6.2:70189731-70189750 None:intergenic 25.0%
!!! CAATCAAAATGATTTTTAGG+TGG - chr6.2:70189935-70189954 MS.gene54235:CDS 25.0%
AATTTATCCATCTCCAATCT+TGG - chr6.2:70189813-70189832 MS.gene54235:CDS 30.0%
AGCCTACATCAAAAAGAATT+TGG - chr6.2:70189312-70189331 MS.gene54235:intron 30.0%
AGGAAATTTCAGTGAAACAT+TGG - chr6.2:70189002-70189021 MS.gene54235:CDS 30.0%
ATATTGGGGCTCAAAATATA+CGG + chr6.2:70189545-70189564 None:intergenic 30.0%
ATCGGATACATATTAGACAT+CGG - chr6.2:70188521-70188540 MS.gene54235:intron 30.0%
ATTGAATTAGCAACCAAGAT+TGG + chr6.2:70189829-70189848 None:intergenic 30.0%
CAACAAAAGACATCTTTGAT+TGG - chr6.2:70190982-70191001 MS.gene54235:CDS 30.0%
CACATATCAACAATATCAAG+TGG - chr6.2:70190912-70190931 MS.gene54235:CDS 30.0%
CTAGTCATCAAGTTTAGATT+TGG - chr6.2:70189769-70189788 MS.gene54235:CDS 30.0%
GAACCAAGTTCATATAACAA+TGG + chr6.2:70190454-70190473 None:intergenic 30.0%
TAATAAGATGGCTACCATAT+GGG + chr6.2:70188803-70188822 None:intergenic 30.0%
TTAAAAACAGAGAAACCCTT+GGG - chr6.2:70187990-70188009 MS.gene54235:CDS 30.0%
TTAATAAGATGGCTACCATA+TGG + chr6.2:70188804-70188823 None:intergenic 30.0%
TTTCCTTGCTTGAGAATATT+GGG + chr6.2:70189560-70189579 None:intergenic 30.0%
! ATTCTTTTTGATGTAGGCTT+TGG + chr6.2:70189311-70189330 None:intergenic 30.0%
! GGTTCATGGTGAAAATATTT+TGG - chr6.2:70189074-70189093 MS.gene54235:intron 30.0%
! GTTGCTTTATAAATCACTCT+TGG - chr6.2:70189996-70190015 MS.gene54235:CDS 30.0%
! TTTTCCTTGCTTGAGAATAT+TGG + chr6.2:70189561-70189580 None:intergenic 30.0%
! TTTTCTTGGTCAAGTTATCT+AGG + chr6.2:70190342-70190361 None:intergenic 30.0%
! TTTTTACAAAGGCCATTGAA+AGG - chr6.2:70189659-70189678 MS.gene54235:CDS 30.0%
!! AAATATTTTGGAAGAGGCTT+TGG - chr6.2:70189086-70189105 MS.gene54235:intron 30.0%
!! ATGAGTGTCTTTTGTTTGAT+CGG - chr6.2:70188503-70188522 MS.gene54235:intron 30.0%
!! CTCTTACTTCTTTTAAGAAG+GGG + chr6.2:70190080-70190099 None:intergenic 30.0%
!! TGGTGAAAATATTTTGGAAG+AGG - chr6.2:70189080-70189099 MS.gene54235:intron 30.0%
!! TTTACTTTTGTTGCAAGAGA+AGG + chr6.2:70189156-70189175 None:intergenic 30.0%
!!! AGTAGTAGAATGTTGCTTTT+GGG - chr6.2:70189513-70189532 MS.gene54235:CDS 30.0%
AATCCAAGCTTATATGAGTG+AGG - chr6.2:70190842-70190861 MS.gene54235:CDS 35.0%
ACGACTTCTTACATTGGAAT+GGG - chr6.2:70190951-70190970 MS.gene54235:CDS 35.0%
ATAGTTAGCATTCAACCATC+TGG + chr6.2:70190868-70190887 None:intergenic 35.0%
ATCCATGGTTATATGAACTC+AGG - chr6.2:70190603-70190622 MS.gene54235:intron 35.0%
ATGATGTGCTAACCTTTCAA+TGG + chr6.2:70189674-70189693 None:intergenic 35.0%
CCAATTGAGTTAAACTTACG+AGG + chr6.2:70188593-70188612 None:intergenic 35.0%
CCTCGTAAGTTTAACTCAAT+TGG - chr6.2:70188590-70188609 MS.gene54235:intron 35.0%
CTTAAAAACAGAGAAACCCT+TGG - chr6.2:70187989-70188008 MS.gene54235:CDS 35.0%
CTTAGATCAGTTTACCCATA+TGG - chr6.2:70188786-70188805 MS.gene54235:intron 35.0%
GAGCAAGAAATGAAGAAAGA+AGG - chr6.2:70190036-70190055 MS.gene54235:intron 35.0%
GATGACACATATGATGCATA+TGG - chr6.2:70189616-70189635 MS.gene54235:intron 35.0%
TAAAGCAACTTCGTGTAATC+CGG + chr6.2:70189985-70190004 None:intergenic 35.0%
TAACTATACACCAACAACAG+CGG - chr6.2:70190881-70190900 MS.gene54235:CDS 35.0%
TACGACTTCTTACATTGGAA+TGG - chr6.2:70190950-70190969 MS.gene54235:CDS 35.0%
TAGGTGGGATATTAATTGCT+TGG - chr6.2:70189951-70189970 MS.gene54235:CDS 35.0%
TGCTATTCATTGCAGACTTA+GGG - chr6.2:70191037-70191056 MS.gene54235:CDS 35.0%
TGGTTATCAAATGGACGTTT+AGG - chr6.2:70190214-70190233 MS.gene54235:intron 35.0%
TTACATTTCCATCTATGAGC+AGG - chr6.2:70189230-70189249 MS.gene54235:intron 35.0%
TTCCTTGCTTGAGAATATTG+GGG + chr6.2:70189559-70189578 None:intergenic 35.0%
TTGTCTTTGTATATAGGTGG+TGG - chr6.2:70189446-70189465 MS.gene54235:CDS 35.0%
! GCAGCAGCATTAACAATTTT+TGG + chr6.2:70191020-70191039 None:intergenic 35.0%
! TCTACTTGCATTGGCAAAAT+TGG - chr6.2:70189272-70189291 MS.gene54235:intron 35.0%
!! ATTCTCTTGCTGTGCTATTT+AGG - chr6.2:70188147-70188166 MS.gene54235:CDS 35.0%
!! ATTGACTCGATATGTCGTTT+GGG - chr6.2:70188026-70188045 MS.gene54235:CDS 35.0%
!! TTCTCTTGCTGTGCTATTTA+GGG - chr6.2:70188148-70188167 MS.gene54235:CDS 35.0%
!! TTCTTTTAAGAAGGGGTCAA+GGG + chr6.2:70190073-70190092 None:intergenic 35.0%
!!! AATCAAGTTGACTTTTCCCA+AGG + chr6.2:70188009-70188028 None:intergenic 35.0%
!!! ATCAAGTTGACTTTTCCCAA+GGG + chr6.2:70188008-70188027 None:intergenic 35.0%
!!! ATTTTTTTTTTGTGGTGGCC+AGG + chr6.2:70188667-70188686 None:intergenic 35.0%
!!! GAGTAGTAGAATGTTGCTTT+TGG - chr6.2:70189512-70189531 MS.gene54235:CDS 35.0%
AACATTGGTCGTAGATGTTG+AGG - chr6.2:70189017-70189036 MS.gene54235:CDS 40.0%
ACATTGGTCGTAGATGTTGA+GGG - chr6.2:70189018-70189037 MS.gene54235:CDS 40.0%
ACCATGAACCATCATATGTG+AGG + chr6.2:70189064-70189083 None:intergenic 40.0%
ACTACTCTATCTCGTGCATA+CGG + chr6.2:70189499-70189518 None:intergenic 40.0%
AGACGTGTTCAACCAATTCA+AGG - chr6.2:70188972-70188991 MS.gene54235:CDS 40.0%
CTGCTATTCATTGCAGACTT+AGG - chr6.2:70191036-70191055 MS.gene54235:CDS 40.0%
GACCTGAGTTCATATAACCA+TGG + chr6.2:70190608-70190627 None:intergenic 40.0%
GTATGAAGCCTCACATATGA+TGG - chr6.2:70189053-70189072 MS.gene54235:intron 40.0%
GTGGGATATTAATTGCTTGG+AGG - chr6.2:70189954-70189973 MS.gene54235:CDS 40.0%
TAATTGCTTGGAGGATCTTC+CGG - chr6.2:70189963-70189982 MS.gene54235:CDS 40.0%
TGCAAGAGAAGGACTCAATT+GGG + chr6.2:70189145-70189164 None:intergenic 40.0%
TGTGCCGCAATATTCATATC+AGG + chr6.2:70187930-70187949 None:intergenic 40.0%
TTAGCAACCAAGATTGGAGA+TGG + chr6.2:70189823-70189842 None:intergenic 40.0%
TTGCAAGAGAAGGACTCAAT+TGG + chr6.2:70189146-70189165 None:intergenic 40.0%
TTGGCTACGACTTCTTACAT+TGG - chr6.2:70190945-70190964 MS.gene54235:CDS 40.0%
!! CTTCTTTTAAGAAGGGGTCA+AGG + chr6.2:70190074-70190093 None:intergenic 40.0%
!! CTTTGTATATAGGTGGTGGA+AGG - chr6.2:70189450-70189469 MS.gene54235:CDS 40.0%
!! GATTGACTCGATATGTCGTT+TGG - chr6.2:70188025-70188044 MS.gene54235:CDS 40.0%
!! TTAGGGTGTTGAGACAACAT+GGG - chr6.2:70188165-70188184 MS.gene54235:intron 40.0%
!! TTTAGGGTGTTGAGACAACA+TGG - chr6.2:70188164-70188183 MS.gene54235:intron 40.0%
AACCAATTCAAGGACGAGCA+AGG - chr6.2:70188982-70189001 MS.gene54235:CDS 45.0%
AAGAAGTCGTAGCCAACACT+GGG + chr6.2:70190941-70190960 None:intergenic 45.0%
AAGAGAAGGACTCAATTGGG+TGG + chr6.2:70189142-70189161 None:intergenic 45.0%
AGCCCCAATATTCTCAAGCA+AGG - chr6.2:70189554-70189573 MS.gene54235:intron 45.0%
ATCAAGTGGTTACCCAGTGT+TGG - chr6.2:70190926-70190945 MS.gene54235:CDS 45.0%
CAAAAACTTGCCTAGGCTAG+TGG - chr6.2:70189200-70189219 MS.gene54235:intron 45.0%
CATAAAATATGCGCGGTCCA+GGG - chr6.2:70188631-70188650 MS.gene54235:intron 45.0%
CTTATATGAGTGAGGCCAGA+TGG - chr6.2:70190850-70190869 MS.gene54235:CDS 45.0%
GCCTCACATATGATGGTTCA+TGG - chr6.2:70189060-70189079 MS.gene54235:intron 45.0%
GGATGAATCCTGCTCATAGA+TGG + chr6.2:70189241-70189260 None:intergenic 45.0%
TAAGAAGTCGTAGCCAACAC+TGG + chr6.2:70190942-70190961 None:intergenic 45.0%
TCATAAAATATGCGCGGTCC+AGG - chr6.2:70188630-70188649 MS.gene54235:intron 45.0%
TGGCCTCACTCATATAAGCT+TGG + chr6.2:70190848-70190867 None:intergenic 45.0%
TTCCTTGCTCGTCCTTGAAT+TGG + chr6.2:70188987-70189006 None:intergenic 45.0%
! CATGGGCTTTATGTTTCACC+TGG - chr6.2:70188182-70188201 MS.gene54235:intron 45.0%
! TAACTTCTTGCCACTAGCCT+AGG + chr6.2:70189213-70189232 None:intergenic 45.0%
!! ATATAGGTGGTGGAAGGAGT+TGG - chr6.2:70189456-70189475 MS.gene54235:CDS 45.0%
!! GTTTTAAATTAGGCAGGGCG+TGG + chr6.2:70189363-70189382 None:intergenic 45.0%
!!! GAGGCTTTGGCTTTTACTTC+TGG - chr6.2:70189099-70189118 MS.gene54235:intron 45.0%
!!! TTTTTTTGTGGTGGCCAGGA+GGG + chr6.2:70188663-70188682 None:intergenic 45.0%
!!! TTTTTTTTGTGGTGGCCAGG+AGG + chr6.2:70188664-70188683 None:intergenic 45.0%
!!! TTTATATTTTGTTTTAAATT+AGG + chr6.2:70189373-70189392 None:intergenic 5.0%
CTCTGCGCAAAAACTTGCCT+AGG - chr6.2:70189193-70189212 MS.gene54235:intron 50.0%
GGATGAAGTTGTGTCTAGCG+AGG - chr6.2:70191058-70191077 MS.gene54235:CDS 50.0%
TGCATGTACTCCGCTGTTGT+TGG + chr6.2:70190894-70190913 None:intergenic 50.0%
TTCTGGTCACCTTCAGTCCA+TGG - chr6.2:70189116-70189135 MS.gene54235:intron 50.0%
AGGACTCAATTGGGTGGCCA+TGG + chr6.2:70189136-70189155 None:intergenic 55.0%
! ATTGGGTGGCCATGGACTGA+AGG + chr6.2:70189128-70189147 None:intergenic 55.0%
GTCCAGGGTTCAAACCCTCC+TGG - chr6.2:70188646-70188665 MS.gene54235:intron 60.0%
GGCCAGGAGGGTTTGAACCC+TGG + chr6.2:70188651-70188670 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 70187924 70191079 70187924 ID=MS.gene54235
chr6.2 mRNA 70187924 70191079 70187924 ID=MS.gene54235.t1;Parent=MS.gene54235
chr6.2 exon 70190800 70191079 70190800 ID=MS.gene54235.t1.exon1;Parent=MS.gene54235.t1
chr6.2 CDS 70190800 70191079 70190800 ID=cds.MS.gene54235.t1;Parent=MS.gene54235.t1
chr6.2 exon 70189657 70190029 70189657 ID=MS.gene54235.t1.exon2;Parent=MS.gene54235.t1
chr6.2 CDS 70189657 70190029 70189657 ID=cds.MS.gene54235.t1;Parent=MS.gene54235.t1
chr6.2 exon 70189323 70189541 70189323 ID=MS.gene54235.t1.exon3;Parent=MS.gene54235.t1
chr6.2 CDS 70189323 70189541 70189323 ID=cds.MS.gene54235.t1;Parent=MS.gene54235.t1
chr6.2 exon 70188911 70189049 70188911 ID=MS.gene54235.t1.exon4;Parent=MS.gene54235.t1
chr6.2 CDS 70188911 70189049 70188911 ID=cds.MS.gene54235.t1;Parent=MS.gene54235.t1
chr6.2 exon 70187924 70188172 70187924 ID=MS.gene54235.t1.exon5;Parent=MS.gene54235.t1
chr6.2 CDS 70187924 70188172 70187924 ID=cds.MS.gene54235.t1;Parent=MS.gene54235.t1
Gene Sequence

>MS.gene54235

GAACCTGATATGAATATTGCGGCACAAATTGATAAACTAAAAAAGGAGGTTAGACAAATGCTTGTCTTAAAAACAGAGAAACCCTTGGGAAAAGTCAACTTGATTGACTCGATATGTCGTTTGGGTGTGAGCTATCACTTTGAAGAAGAAATTAGTCAAGTTTTGGAACATATTTACAAGAGTTATGTTCAAAATGGAGAAATAACTCTCAAAGACAATCTTCATTCTCTTGCTGTGCTATTTAGGGTGTTGAGACAACATGGGCTTTATGTTTCACCTGGTATATATATCTCAACTTATTCACATTTTTTTTTAAGGAATCACAACTTATTCACATTAATGATTCATTTTTTATGTTTTTAATTTGGATCTCAAATTGAGTAAAACAAATTAGATTGCTAAACATAAAAAAAAATATCTAATTGTAAAATCAATACTTCAATTAACAACGTAATATTTGACTATATTCATGGGTGTGTTTAATATGAATGTCTAAAAAATGGTAGACCACTTTTAGTAAAAAAAGGATTTTATAATAATAAAAGATTGAAAATAATTTTTATATTTTTAACAAAAAATATGAGTGTCTTTTGTTTGATCGGATACATATTAGACATCGGTGTAATGTTCATATCCTCATTTCTTTTAATTATTATCTTTTAATGTCCTCGTAAGTTTAACTCAATTGGTTTGGACAATTCATAAATCATAAAATATGCGCGGTCCAGGGTTCAAACCCTCCTGGCCACCACAAAAAAAAAATTATTATCTTTTAATTATATTTTTTTATATGATAGAAATAAACAATAAATGATTATAAAGTAATATTAATGTTATACATGTAGGATAGTCATCTTTATAACTTAGATCAGTTTACCCATATGGTAGCCATCTTATTAAGAAACTTTTTCGTACTTCAAAAACACACTATACATGATCTTAATTGCATACTGAATTTTTCAATCCTGTATTAGTTTCTTATTTATATATAAATTAATATGTCTCTAATGGTTGTTTAATATTATTGTGGTTGTCTAATAAAAATACTAGACGTGTTCAACCAATTCAAGGACGAGCAAGGAAATTTCAGTGAAACATTGGTCGTAGATGTTGAGGGCATGTTAAACTTGTATGAAGCCTCACATATGATGGTTCATGGTGAAAATATTTTGGAAGAGGCTTTGGCTTTTACTTCTGGTCACCTTCAGTCCATGGCCACCCAATTGAGTCCTTCTCTTGCAACAAAAGTAAAACAAAGCTTAAGACAATCTCTGCGCAAAAACTTGCCTAGGCTAGTGGCAAGAAGTTACATTTCCATCTATGAGCAGGATTCATCCCATAATGAAATTCTACTTGCATTGGCAAAATTGGATTTTAATATGCTCCAAAGCCTACATCAAAAAGAATTTGGAAATATTAGCAAGTAAGTACTTTATCCACGCCCTGCCTAATTTAAAACAAAATATAAAAGTATATTAAATTGAGTTCAAAAAATAATTATACTAATTTCCTATACTATTTTTTGTCTTTGTATATAGGTGGTGGAAGGAGTTGGATATTGCACAAAAACTACCGTATGCACGAGATAGAGTAGTAGAATGTTGCTTTTGGGTTATGACCGTATATTTTGAGCCCCAATATTCTCAAGCAAGGAAAATGATGTCAAAATTTATCTCCATTTTATCATACATTGATGACACATATGATGCATATGGAACTATTGATGAATTAGAACTTTTTACAAAGGCCATTGAAAGGTTAGCACATCATACAATATTAATGTTAAGAATAATTGAATTCGTGACCAAACTTTAGAGTTAAAACCATATGAAATTTATCTTTAATCTAGTCATCAAGTTTAGATTTGGAAATTTTTTAATAATAACTTCAATTTATCCATCTCCAATCTTGGTTGCTAATTCAATTTTTGTTAAGAAATGTAGACATATTTTCTTCACATATAATTGTACTCGATATGATTTTGATAACAATTGAGTAATGATTAATATTGCAATCAAAATGATTTTTAGGTGGGATATTAATTGCTTGGAGGATCTTCCGGATTACACGAAGTTGCTTTATAAATCACTCTTGGATGTTTATGAAGAAATAGAGCAAGAAATGAAGAAAGAAGGAAGAGAATACACCCTTGACCCCTTCTTAAAAGAAGTAAGAGTTTAGCTTTATTGTTAGTATTATTAGTAATAATAAAGCATTAGTTAAAATTTGTTTTTTTATTGATTTATAATTTATTCAACAATGGAACAAAGATTTCATTTTAAATTTTGTATGGTTATCAAATGGACGTTTAGGATTTTATTTGTACACACTATCAATTTAAATACATGTATCATATATTATCTCAACTTTAAAATATTTTTACATCATTTTAGGAATTAACATGATAAAGTATCACCTAGATAACTTGACCAAGAAAAATATATTATTATGAAGTTATTTGATTGGATGCAATGTTAAAAAAATTTCTAATATCAAATAATAAAAAATAATGTAAAATGCTTATTATCCATTGTTATATGAACTTGGTTCATTAACATGATAAAGTATCATCATGATGACTTGACAAAGAAAAATGTTTCATCATGAGGTTATTTGATTAAATGCAATGTTAAAAAGTTTTCGACTATCAAAGAATAAAAATTAAGGTAAAAACTTATTATCCATGGTTATATGAACTCAGGTCAGAGTAATGAAGATGATAAATTTTGTTTTGTCGTAAAAGTGCTCCTTTGTAATCTTAATTTTATGAAAGATATTTCTTTATAGTTTAATCGGTGGTTAGATAAAAACTAAAAACAATTGCTAATACAATTTAACATCATTTAATTACATAGGTTTTTTTGGTACATAAATTAACATTTATTTTATATTGTCTACTAATGACAGTTCAAAAATATAATCCAAGCTTATATGAGTGAGGCCAGATGGTTGAATGCTAACTATACACCAACAACAGCGGAGTACATGCACATATCAACAATATCAAGTGGTTACCCAGTGTTGGCTACGACTTCTTACATTGGAATGGGAAACATAGCAACAAAAGACATCTTTGATTGGGTAACTAACAATCCAAAAATTGTTAATGCTGCTGCTATTCATTGCAGACTTAGGGATGAAGTTGTGTCTAGCGAG
Protein sequence

>MS.gene54235.t1

EPDMNIAAQIDKLKKEVRQMLVLKTEKPLGKVNLIDSICRLGVSYHFEEEISQVLEHIYKSYVQNGEITLKDNLHSLAVLFRVLRQHGLYVSPDVFNQFKDEQGNFSETLVVDVEGMLNLYEASHMMVHGENILEEALAFTSGHLQSMATQLSPSLATKVKQSLRQSLRKNLPRLVARSYISIYEQDSSHNEILLALAKLDFNMLQSLHQKEFGNISKWWKELDIAQKLPYARDRVVECCFWVMTVYFEPQYSQARKMMSKFISILSYIDDTYDAYGTIDELELFTKAIERWDINCLEDLPDYTKLLYKSLLDVYEEIEQEMKKEGREYTLDPFLKEFKNIIQAYMSEARWLNANYTPTTAEYMHISTISSGYPVLATTSYIGMGNIATKDIFDWVTNNPKIVNAAAIHCRLRDEVVSSE