AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54295

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54295.t1 MTR_2g016160 99.686 318 1 0 1 318 1 318 0.0 653
MS.gene54295.t1 MTR_4g073080 26.891 238 164 4 47 274 99 336 3.96e-20 90.5
MS.gene54295.t1 MTR_4g119620 26.047 215 154 5 96 309 67 277 3.07e-19 87.0
MS.gene54295.t1 MTR_4g119620 24.000 275 195 10 45 314 61 326 2.47e-12 67.0
MS.gene54295.t1 MTR_4g096880 23.411 299 187 6 54 310 105 403 9.48e-19 86.7
MS.gene54295.t1 MTR_4g096880 24.571 175 117 2 64 223 230 404 1.37e-11 65.5
MS.gene54295.t1 MTR_6g004040 27.670 206 141 6 61 266 59 256 3.62e-17 82.4
MS.gene54295.t1 MTR_2g012630 28.436 211 143 7 100 309 8 211 4.91e-17 82.4
MS.gene54295.t1 MTR_1g072140 29.412 187 126 5 125 309 86 268 7.93e-17 80.1
MS.gene54295.t1 MTR_7g104550 33.333 180 103 7 100 265 9 185 9.00e-17 80.1
MS.gene54295.t1 MTR_5g016350 29.670 182 113 7 97 269 408 583 2.27e-16 80.1
MS.gene54295.t1 MTR_2g102277 30.000 190 125 6 78 265 73 256 2.50e-16 80.1
MS.gene54295.t1 MTR_2g102277 30.000 190 125 6 78 265 73 256 2.84e-16 80.1
MS.gene54295.t1 MTR_5g016350 29.670 182 113 7 97 269 344 519 3.78e-16 79.3
MS.gene54295.t1 MTR_2g012630 28.436 211 143 7 100 309 8 211 7.46e-16 79.0
MS.gene54295.t1 MTR_4g011950 29.474 190 126 6 78 265 73 256 8.25e-16 78.6
MS.gene54295.t1 MTR_4g095058 29.121 182 114 7 97 269 403 578 2.19e-15 77.0
MS.gene54295.t1 MTR_4g095058 29.121 182 114 7 97 269 401 576 2.33e-15 77.0
MS.gene54295.t1 MTR_8g027985 25.843 267 172 10 60 310 649 905 2.38e-15 77.4
MS.gene54295.t1 MTR_8g080700 28.022 182 112 6 99 267 216 391 2.45e-15 76.6
MS.gene54295.t1 MTR_3g106080 28.700 223 126 9 72 267 358 574 3.38e-15 76.6
MS.gene54295.t1 MTR_5g097870 26.667 225 153 3 62 277 252 473 1.19e-14 74.7
MS.gene54295.t1 MTR_5g097870 27.811 169 115 1 106 267 250 418 2.18e-11 64.7
MS.gene54295.t1 MTR_1g050340 27.193 228 153 8 83 310 573 787 1.34e-14 74.7
MS.gene54295.t1 MTR_1g050340 27.193 228 153 8 83 310 572 786 1.36e-14 74.7
MS.gene54295.t1 MTR_5g016370 27.222 180 116 7 99 269 422 595 1.49e-14 74.7
MS.gene54295.t1 MTR_3g045130 25.664 226 159 7 84 309 294 510 2.17e-14 73.9
MS.gene54295.t1 MTR_3g045130 27.586 203 133 7 66 265 319 510 2.64e-14 73.6
MS.gene54295.t1 MTR_7g086360 29.452 146 99 4 122 267 112 253 3.52e-14 72.8
MS.gene54295.t1 MTR_5g009530 30.323 155 105 2 113 267 776 927 1.28e-13 72.0
MS.gene54295.t1 MTR_5g022690 26.613 248 155 10 41 273 163 398 1.37e-13 71.6
MS.gene54295.t1 MTR_5g009530 30.323 155 105 2 113 267 776 927 1.49e-13 72.0
MS.gene54295.t1 MTR_3g092840 27.568 185 127 4 112 292 138 319 1.61e-12 67.8
MS.gene54295.t1 MTR_2g085210 27.053 207 140 5 109 309 616 817 2.98e-12 67.8
MS.gene54295.t1 MTR_5g097870 27.835 194 128 3 62 246 252 442 4.30e-12 67.0
MS.gene54295.t1 MTR_5g097870 27.811 169 115 1 106 267 250 418 1.97e-11 65.1
MS.gene54295.t1 MTR_5g024090 30.319 188 105 9 99 269 210 388 4.87e-12 66.6
MS.gene54295.t1 MTR_4g105700 26.582 237 154 9 85 309 383 611 1.49e-11 65.9
MS.gene54295.t1 MTR_3g115200 21.014 276 194 7 10 265 9 280 3.61e-11 63.2
MS.gene54295.t1 MTR_3g115200 21.014 276 194 7 10 265 9 280 5.84e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54295.t1 AT1G29260 81.191 319 57 2 1 318 1 317 0.0 558
MS.gene54295.t1 AT4G35050 26.809 235 159 4 46 267 101 335 1.35e-20 92.0
MS.gene54295.t1 AT5G58230 23.000 300 189 6 53 310 104 403 6.27e-20 90.1
MS.gene54295.t1 AT5G58230 24.000 175 118 2 64 223 230 404 1.22e-11 65.5
MS.gene54295.t1 AT3G49660 26.050 238 169 6 76 310 38 271 2.11e-19 87.4
MS.gene54295.t1 AT2G16780 24.153 236 166 3 45 267 99 334 1.10e-17 83.6
MS.gene54295.t1 AT2G16780 21.888 233 153 6 10 223 171 393 1.92e-11 64.7
MS.gene54295.t1 AT2G26060 35.519 183 96 9 100 265 13 190 1.08e-16 80.1
MS.gene54295.t1 AT2G26060 35.519 183 96 9 100 265 13 190 1.29e-16 79.7
MS.gene54295.t1 AT5G08390 25.926 216 152 6 51 266 49 256 1.40e-16 80.9
MS.gene54295.t1 AT5G23430 25.926 216 152 6 51 266 49 256 1.89e-16 80.5
MS.gene54295.t1 AT5G23430 25.926 216 152 6 51 266 49 256 1.89e-16 80.5
MS.gene54295.t1 AT5G23430 25.926 216 152 6 51 266 49 256 1.96e-16 80.5
MS.gene54295.t1 AT2G05720 28.054 221 128 7 75 290 76 270 3.10e-16 77.8
MS.gene54295.t1 AT3G15980 30.000 190 125 6 78 265 73 256 4.21e-16 79.7
MS.gene54295.t1 AT3G15980 30.000 190 125 6 78 265 73 256 4.27e-16 79.3
MS.gene54295.t1 AT3G15980 30.000 190 125 6 78 265 73 256 4.27e-16 79.3
MS.gene54295.t1 AT3G15980 30.000 190 125 6 78 265 73 256 4.27e-16 79.3
MS.gene54295.t1 AT3G15980 30.000 190 125 6 78 265 73 256 4.27e-16 79.3
MS.gene54295.t1 AT3G15980 30.000 190 125 6 78 265 73 256 4.42e-16 79.3
MS.gene54295.t1 AT3G15980 30.000 190 125 6 78 265 73 256 4.50e-16 79.3
MS.gene54295.t1 AT5G67320 29.670 182 113 7 97 269 438 613 9.08e-16 78.2
MS.gene54295.t1 AT1G52360 29.474 190 126 6 78 265 73 256 9.11e-16 78.6
MS.gene54295.t1 AT1G52360 29.474 190 126 6 78 265 73 256 9.11e-16 78.6
MS.gene54295.t1 AT1G52360 29.474 190 126 6 78 265 117 300 1.24e-15 78.2
MS.gene54295.t1 AT1G52360 29.474 190 126 6 78 265 73 256 1.24e-15 78.2
MS.gene54295.t1 AT1G79990 30.000 190 125 6 78 265 73 256 1.92e-15 77.4
MS.gene54295.t1 AT1G79990 30.000 190 125 6 78 265 73 256 2.06e-15 77.4
MS.gene54295.t1 AT5G25150 28.626 262 154 11 72 309 361 613 4.26e-15 76.3
MS.gene54295.t1 AT5G25150 28.626 262 154 11 72 309 361 613 4.66e-15 76.3
MS.gene54295.t1 AT2G19540 27.374 179 117 4 99 267 215 390 9.11e-15 75.1
MS.gene54295.t1 AT1G61210 26.190 210 149 6 100 309 8 211 1.51e-14 75.1
MS.gene54295.t1 AT1G61210 25.500 200 140 6 71 269 67 258 1.03e-13 72.4
MS.gene54295.t1 AT1G61210 26.190 210 149 6 100 309 8 211 2.18e-14 74.3
MS.gene54295.t1 AT1G61210 25.500 200 140 6 71 269 67 258 1.70e-13 71.6
MS.gene54295.t1 AT1G61210 26.190 210 149 6 100 309 8 211 2.22e-14 74.3
MS.gene54295.t1 AT1G61210 25.500 200 140 6 71 269 67 258 1.84e-13 71.6
MS.gene54295.t1 AT4G18900 30.952 168 110 1 106 267 232 399 5.12e-14 72.8
MS.gene54295.t1 AT4G18900 27.803 223 148 5 64 277 236 454 5.56e-14 72.8
MS.gene54295.t1 AT2G43770 30.822 146 97 4 122 267 110 251 7.73e-14 71.6
MS.gene54295.t1 AT2G43770 27.570 214 136 9 62 266 97 300 4.14e-11 63.5
MS.gene54295.t1 AT1G11160 24.623 199 141 6 72 269 68 258 2.09e-13 71.2
MS.gene54295.t1 AT1G11160 24.623 199 141 6 72 269 68 258 2.15e-13 71.2
MS.gene54295.t1 AT1G11160 24.623 199 141 6 72 269 26 216 2.23e-13 71.2
MS.gene54295.t1 AT1G11160 24.623 199 141 6 72 269 68 258 2.34e-13 71.2
MS.gene54295.t1 AT2G32950 27.602 221 147 6 90 309 296 504 3.03e-13 70.5
MS.gene54295.t1 AT2G32950 27.602 221 147 6 90 309 409 617 4.11e-13 70.5
MS.gene54295.t1 AT4G11110 25.571 219 143 5 59 267 721 929 8.49e-13 69.7
MS.gene54295.t1 AT2G46340 27.184 206 135 5 113 309 771 970 1.41e-12 68.9
MS.gene54295.t1 AT2G46340 27.184 206 135 5 113 309 771 970 1.41e-12 68.9
MS.gene54295.t1 AT4G18905 26.754 228 151 5 62 277 266 489 1.82e-12 68.2
MS.gene54295.t1 AT4G02730 25.595 168 119 6 100 266 36 198 1.93e-12 67.4
MS.gene54295.t1 AT4G18905 26.754 228 151 5 62 277 276 499 2.08e-12 68.2
MS.gene54295.t1 AT4G35370 26.455 189 135 2 83 267 189 377 3.75e-12 67.0
MS.gene54295.t1 AT4G35370 27.461 193 134 3 64 253 219 408 1.42e-11 65.5
MS.gene54295.t1 AT5G24520 28.144 167 114 3 105 267 130 294 5.41e-12 66.2
MS.gene54295.t1 AT5G24520 28.144 167 114 3 105 267 130 294 5.41e-12 66.2
MS.gene54295.t1 AT5G24520 28.144 167 114 3 105 267 130 294 5.41e-12 66.2
MS.gene54295.t1 AT5G24520 28.144 167 114 3 105 267 130 294 5.41e-12 66.2
MS.gene54295.t1 AT3G16650 29.213 178 117 7 82 259 190 358 2.14e-11 64.7
MS.gene54295.t1 AT1G20540 26.087 184 128 5 87 265 100 280 2.56e-11 64.3
MS.gene54295.t1 AT3G15354 25.728 206 144 4 109 309 335 536 5.04e-11 63.9
MS.gene54295.t1 AT3G15354 25.728 206 144 4 109 309 335 536 5.04e-11 63.9
MS.gene54295.t1 AT3G15354 25.728 206 144 4 109 309 585 786 7.24e-11 63.5
MS.gene54295.t1 AT4G15900 24.561 228 161 7 82 309 196 412 7.32e-11 63.2
MS.gene54295.t1 AT5G13480 27.972 143 100 3 123 265 233 372 8.37e-11 63.2
MS.gene54295.t1 AT5G13480 27.972 143 100 3 123 265 222 361 8.94e-11 63.2
MS.gene54295.t1 AT5G13480 27.972 143 100 3 123 265 210 349 9.62e-11 63.2
MS.gene54295.t1 AT5G13480 27.972 143 100 3 123 265 210 349 9.70e-11 63.2

Find 92 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGATTGTACTGTCAGAATT+TGG 0.134510 2.3:-69305354 MS.gene54295:CDS
GTTCCGGTTGCCGTGCTTAA+TGG 0.204077 2.3:-69304427 MS.gene54295:CDS
TTTGGGATGTAAGGAGTTAT+AGG 0.273478 2.3:-69304450 MS.gene54295:CDS
GGGACGATACCGTCAAATTA+TGG 0.291682 2.3:-69305483 MS.gene54295:CDS
TTCCGGTTGCCGTGCTTAAT+GGG 0.295179 2.3:-69304426 MS.gene54295:CDS
ATGATATGACTGTTTGTGTT+TGG 0.299620 2.3:-69304336 MS.gene54295:CDS
ATTGTGTTTATTCATCTGTT+TGG 0.320363 2.3:-69305414 MS.gene54295:CDS
TTGGGATGTAAGGAGTTATA+GGG 0.332484 2.3:-69304449 MS.gene54295:CDS
TGATATGACTGTTTGTGTTT+GGG 0.333313 2.3:-69304335 MS.gene54295:CDS
CAGTTAAGGTTTGGGATGTA+AGG 0.354149 2.3:-69304459 MS.gene54295:CDS
TTGATAAGTCAGTTAAGGTT+TGG 0.355478 2.3:-69304468 MS.gene54295:CDS
TAGGCGGCGATTTCGGTGAT+TGG 0.373569 2.3:+69305715 None:intergenic
TGATAAGTCAGTTAAGGTTT+GGG 0.383380 2.3:-69304467 MS.gene54295:CDS
ATCATACTGAGTTTGCTGTT+GGG 0.389414 2.3:-69304273 MS.gene54295:CDS
TGTTCATGAAAGGAACGAAT+AGG 0.390721 2.3:+69305571 None:intergenic
GGGATGAACTTGTTTATGTT+TGG 0.393483 2.3:-69304204 MS.gene54295:CDS
GGTGATTGGGAGAGTGGGGT+TGG 0.394635 2.3:+69305729 None:intergenic
GATGAATGTATAATTGCAAC+TGG 0.406242 2.3:-69304496 MS.gene54295:CDS
TGTAAGGAGTTATAGGGTTC+CGG 0.408160 2.3:-69304443 MS.gene54295:CDS
GCTTCTTGCTAGTACTGGTT+GGG 0.412462 2.3:-69304224 MS.gene54295:CDS
ATGAGATTCTTTCGTGCGAT+TGG 0.420567 2.3:-69304528 MS.gene54295:CDS
GATATGAGTGTTCTTGTTGA+AGG 0.426691 2.3:-69304247 MS.gene54295:CDS
TGACGGAGTAACCGTTGAAT+GGG 0.426922 2.3:+69305848 None:intergenic
TGCCCATTAAGCACGGCAAC+CGG 0.427081 2.3:+69304424 None:intergenic
GTCGGTTGATAAGTCAGTTA+AGG 0.429070 2.3:-69304473 MS.gene54295:CDS
GGCTTCTTGCTAGTACTGGT+TGG 0.432245 2.3:-69304225 MS.gene54295:CDS
AGAAACGAATCACGGCGAAC+AGG 0.432589 2.3:+69305517 None:intergenic
TTGACGGAGTAACCGTTGAA+TGG 0.442266 2.3:+69305847 None:intergenic
GGATTGTACTGTCAGAATTT+GGG 0.443764 2.3:-69305353 MS.gene54295:CDS
GAAGGGCTTCTTGCTAGTAC+TGG 0.444363 2.3:-69304229 MS.gene54295:CDS
ATTTCGGTGATTGGGAGAGT+GGG 0.454712 2.3:+69305724 None:intergenic
GATTGGGAGAGTGGGGTTGG+AGG 0.460624 2.3:+69305732 None:intergenic
CTTGTTTATGTTTGGCAACA+TGG 0.461447 2.3:-69304196 MS.gene54295:CDS
GGTGTCGTAGGCGGCGATTT+CGG 0.462174 2.3:+69305708 None:intergenic
GTTCATGAAAGGAACGAATA+GGG 0.469484 2.3:+69305572 None:intergenic
GTAGAAGGGGCTGAATTTGA+CGG 0.470246 2.3:+69305831 None:intergenic
CATCATACTGAGTTTGCTGT+TGG 0.478915 2.3:-69304274 MS.gene54295:CDS
AGGCGGCGATTTCGGTGATT+GGG 0.479284 2.3:+69305716 None:intergenic
TGGAGGAGAGTTCGAGGACG+TGG 0.479460 2.3:+69305749 None:intergenic
GGCAACGGCGGCGACGGTAA+TGG 0.482055 2.3:+69305642 None:intergenic
ACCGTCGCCGCCGTTGCCGA+TGG 0.484087 2.3:-69305637 MS.gene54295:CDS
ATTTGGGATGTTCGTGAACC+AGG 0.485985 2.3:-69305337 MS.gene54295:intron
TCATACTGAGTTTGCTGTTG+GGG 0.495869 2.3:-69304272 MS.gene54295:CDS
TGGGGTTGGAGGAGAGTTCG+AGG 0.501933 2.3:+69305743 None:intergenic
ATATGAGTGTTCTTGTTGAA+GGG 0.514929 2.3:-69304246 MS.gene54295:CDS
AGGTTCTACAATGATTCTTC+CGG 0.515402 2.3:-69304560 MS.gene54295:CDS
GCCGCATGTGAGGAATTTGT+TGG 0.515565 2.3:-69304371 MS.gene54295:CDS
GCATGGTTATGCTGTTAGAA+AGG 0.517131 2.3:-69304404 MS.gene54295:CDS
GATGCCGTCGGCGGTGTCGT+AGG 0.517224 2.3:+69305696 None:intergenic
GGCAACATGGAACTGATCCT+AGG 0.522032 2.3:-69304183 MS.gene54295:CDS
TTGAACGTTCTCACCGAAGT+GGG 0.523846 2.3:+69305448 None:intergenic
GATTTCGGTGATTGGGAGAG+TGG 0.524323 2.3:+69305723 None:intergenic
ACCATCGGCAACGGCGGCGA+CGG 0.530414 2.3:+69305636 None:intergenic
AGCGAGGTCGTAAAGTTTGA+CGG 0.535236 2.3:+69305612 None:intergenic
CTTCCCTTTCAAGATGCCCT+AGG 0.540302 2.3:+69304166 None:intergenic
GTCGCCACCGCTCAAAACTT+CGG 0.541735 2.3:-69305793 MS.gene54295:CDS
GTTGCCGTGCTTAATGGGCA+TGG 0.541849 2.3:-69304421 MS.gene54295:CDS
ATCCACGGTCCATAATTTGA+CGG 0.542435 2.3:+69305474 None:intergenic
AACTTCGGCATCCTCGGCAA+CGG 0.545359 2.3:-69305778 MS.gene54295:CDS
AGAATCTCATGTTCATGTGC+CGG 0.550681 2.3:+69304541 None:intergenic
TTCGTTTCTCTCCGCTGCGT+GGG 0.552872 2.3:-69305503 MS.gene54295:CDS
GCTCAAAACTTCGGCATCCT+CGG 0.554172 2.3:-69305784 MS.gene54295:CDS
CGAATAGGGTTTGAAGTAGG+TGG 0.559454 2.3:+69305586 None:intergenic
ATTCGTTTCTCTCCGCTGCG+TGG 0.561476 2.3:-69305504 MS.gene54295:CDS
CACCGAAGTGGGACGATCCA+CGG 0.563775 2.3:+69305459 None:intergenic
GTAAAGTTTGACGGAACCAT+CGG 0.567165 2.3:+69305621 None:intergenic
GCAACATGGAACTGATCCTA+GGG 0.574620 2.3:-69304182 MS.gene54295:CDS
CGTGGAGACGGCCGTTGCCG+AGG 0.577422 2.3:+69305767 None:intergenic
GACGGAACCATCGGCAACGG+CGG 0.578656 2.3:+69305630 None:intergenic
AGAGTTCGAGGACGTGGAGA+CGG 0.581734 2.3:+69305755 None:intergenic
GAGCGGTGGCGACGGCGATG+CGG 0.585400 2.3:+69305803 None:intergenic
GCCGCCTACGACACCGCCGA+CGG 0.585566 2.3:-69305700 MS.gene54295:CDS
GCCGTCGGCGGTGTCGTAGG+CGG 0.589550 2.3:+69305699 None:intergenic
TTCACGTGTATGTTCATGAA+AGG 0.590355 2.3:+69305561 None:intergenic
GACCGTGGATCGTCCCACTT+CGG 0.593212 2.3:-69305461 MS.gene54295:CDS
TTTGAACGTTCTCACCGAAG+TGG 0.595208 2.3:+69305447 None:intergenic
TTTCGGTGATTGGGAGAGTG+GGG 0.608168 2.3:+69305725 None:intergenic
GAACGAATAGGGTTTGAAGT+AGG 0.614418 2.3:+69305583 None:intergenic
TTTGACGGAACCATCGGCAA+CGG 0.622318 2.3:+69305627 None:intergenic
ATAACCATGCCCATTAAGCA+CGG 0.633480 2.3:+69304417 None:intergenic
GACGGAGTAACCGTTGAATG+GGG 0.634585 2.3:+69305849 None:intergenic
ACCAACAAATTCCTCACATG+CGG 0.642131 2.3:+69304370 None:intergenic
GACGGTATCGTCCCACGCAG+CGG 0.642717 2.3:+69305492 None:intergenic
ACGGCATCTACGACGTCACA+TGG 0.642993 2.3:-69305681 MS.gene54295:CDS
CAGCGGAGAGAAACGAATCA+CGG 0.652885 2.3:+69305509 None:intergenic
TACCGTCAAATTATGGACCG+TGG 0.654878 2.3:-69305476 MS.gene54295:CDS
TTGAAGTAGGTGGAAGAGCG+AGG 0.660138 2.3:+69305596 None:intergenic
GACGTCGTAGATGCCGTCGG+CGG 0.671920 2.3:+69305687 None:intergenic
TGTGACGTCGTAGATGCCGT+CGG 0.679756 2.3:+69305684 None:intergenic
ATGTATAATTGCAACTGGGT+CGG 0.683430 2.3:-69304491 MS.gene54295:CDS
GCGAACAGGATTATAATCAA+CGG 0.693874 2.3:+69305531 None:intergenic
ATGAATGTATAATTGCAACT+GGG 0.763887 2.3:-69304495 MS.gene54295:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAATATATTTTGACATTTT+TGG + chr2.3:69305296-69305315 None:intergenic 15.0%
!! AATCCATTAAAGCAAAATTA+GGG - chr2.3:69304960-69304979 MS.gene54295:intron 20.0%
!! ATTACTTAAAAACCTAGTAT+TGG + chr2.3:69305417-69305436 None:intergenic 20.0%
!! TACTTAAATTAGTGTATGTT+TGG + chr2.3:69305358-69305377 None:intergenic 20.0%
!! TATATCAAAATTGTAATACC+TGG + chr2.3:69304723-69304742 None:intergenic 20.0%
!!! AGAATTGATTTTGATATGTT+TGG + chr2.3:69305203-69305222 None:intergenic 20.0%
!!! ATAGTTCAACTAACTTTTAA+GGG + chr2.3:69305081-69305100 None:intergenic 20.0%
!!! TATAGTTCAACTAACTTTTA+AGG + chr2.3:69305082-69305101 None:intergenic 20.0%
!!! TTTTAGCTATAGTTAAGTTA+AGG - chr2.3:69304876-69304895 MS.gene54295:intron 20.0%
! ATCCATTAAAGCAAAATTAG+GGG - chr2.3:69304961-69304980 MS.gene54295:intron 25.0%
! ATGAATGTATAATTGCAACT+GGG - chr2.3:69305544-69305563 MS.gene54295:CDS 25.0%
! ATTGTGTTTATTCATCTGTT+TGG - chr2.3:69304625-69304644 MS.gene54295:intron 25.0%
! GAATCCATTAAAGCAAAATT+AGG - chr2.3:69304959-69304978 MS.gene54295:intron 25.0%
! TAACTATTACATCCAATACT+AGG - chr2.3:69305402-69305421 MS.gene54295:CDS 25.0%
! TATCTATTACATCTAATACC+AGG - chr2.3:69305459-69305478 MS.gene54295:CDS 25.0%
!! TCTAAGTGTATGTTTCATTT+TGG - chr2.3:69304806-69304825 MS.gene54295:intron 25.0%
AAGTAGAATCAGTGTATGTT+TGG + chr2.3:69305265-69305284 None:intergenic 30.0%
ATATGAGTGTTCTTGTTGAA+GGG - chr2.3:69305793-69305812 MS.gene54295:CDS 30.0%
ATGATATGACTGTTTGTGTT+TGG - chr2.3:69305703-69305722 MS.gene54295:CDS 30.0%
GATGAATGTATAATTGCAAC+TGG - chr2.3:69305543-69305562 MS.gene54295:CDS 30.0%
TGATAAGTCAGTTAAGGTTT+GGG - chr2.3:69305572-69305591 MS.gene54295:CDS 30.0%
TGATATGACTGTTTGTGTTT+GGG - chr2.3:69305704-69305723 MS.gene54295:CDS 30.0%
TGTAAAAATGAGCTGAACAT+CGG - chr2.3:69304917-69304936 MS.gene54295:intron 30.0%
TTGATAAGTCAGTTAAGGTT+TGG - chr2.3:69305571-69305590 MS.gene54295:CDS 30.0%
! TACCCCTAATTTTGCTTTAA+TGG + chr2.3:69304966-69304985 None:intergenic 30.0%
!! ATACACTGATTCTACTTTAG+AGG - chr2.3:69305268-69305287 MS.gene54295:intron 30.0%
!! ATACGGGTTAGAATTGATTA+TGG + chr2.3:69305232-69305251 None:intergenic 30.0%
!! TGTTTGTGTTTGGGATTTTA+TGG - chr2.3:69305713-69305732 MS.gene54295:CDS 30.0%
!!! AATTTTGACTCTTCCAAAAG+TGG - chr2.3:69305330-69305349 MS.gene54295:intron 30.0%
AATTCTAACCCGTATAGAAG+TGG - chr2.3:69305237-69305256 MS.gene54295:intron 35.0%
AGGTTCTACAATGATTCTTC+CGG - chr2.3:69305479-69305498 MS.gene54295:CDS 35.0%
ATGTATAATTGCAACTGGGT+CGG - chr2.3:69305548-69305567 MS.gene54295:CDS 35.0%
CTTGTTTATGTTTGGCAACA+TGG - chr2.3:69305843-69305862 MS.gene54295:CDS 35.0%
GATATGAGTGTTCTTGTTGA+AGG - chr2.3:69305792-69305811 MS.gene54295:CDS 35.0%
GCGAACAGGATTATAATCAA+CGG + chr2.3:69304511-69304530 None:intergenic 35.0%
GCTTCAAGCAGAATCAATTA+TGG - chr2.3:69305149-69305168 MS.gene54295:intron 35.0%
GGATTGTACTGTCAGAATTT+GGG - chr2.3:69304686-69304705 MS.gene54295:intron 35.0%
GGGTTAGAATTGATTATGGA+GGG + chr2.3:69305228-69305247 None:intergenic 35.0%
GTTCATGAAAGGAACGAATA+GGG + chr2.3:69304470-69304489 None:intergenic 35.0%
TCAAGCAGAATCAATTATGG+AGG - chr2.3:69305152-69305171 MS.gene54295:intron 35.0%
TGTTCATGAAAGGAACGAAT+AGG + chr2.3:69304471-69304490 None:intergenic 35.0%
TTCACGTGTATGTTCATGAA+AGG + chr2.3:69304481-69304500 None:intergenic 35.0%
TTGGGATGTAAGGAGTTATA+GGG - chr2.3:69305590-69305609 MS.gene54295:CDS 35.0%
! GGGATGAACTTGTTTATGTT+TGG - chr2.3:69305835-69305854 MS.gene54295:CDS 35.0%
! GTTTTCAAAACCCCATTCAA+CGG - chr2.3:69304180-69304199 MS.gene54295:CDS 35.0%
! TGTGACTAAAAGTGAGTTGA+AGG - chr2.3:69305029-69305048 MS.gene54295:intron 35.0%
! TGTTTGGTTCCACTTCTATA+CGG + chr2.3:69305249-69305268 None:intergenic 35.0%
! TTTGGGATGTAAGGAGTTAT+AGG - chr2.3:69305589-69305608 MS.gene54295:CDS 35.0%
!! ATCATACTGAGTTTGCTGTT+GGG - chr2.3:69305766-69305785 MS.gene54295:CDS 35.0%
!! TGTATGTTTGGTTCCACTTT+TGG + chr2.3:69305346-69305365 None:intergenic 35.0%
!! TTGTGTTTGGGATTTTATGG+TGG - chr2.3:69305716-69305735 MS.gene54295:CDS 35.0%
!! TTTTATGGTGGAAGATGCTT+TGG - chr2.3:69305728-69305747 MS.gene54295:CDS 35.0%
!!! TTGAATGGGGTTTTGAAAAC+TGG + chr2.3:69304180-69304199 None:intergenic 35.0%
ACCAACAAATTCCTCACATG+CGG + chr2.3:69305672-69305691 None:intergenic 40.0%
AGAAGCCGATGCAAAAACAT+CGG + chr2.3:69304664-69304683 None:intergenic 40.0%
AGAATCTCATGTTCATGTGC+CGG + chr2.3:69305501-69305520 None:intergenic 40.0%
ATAACCATGCCCATTAAGCA+CGG + chr2.3:69305625-69305644 None:intergenic 40.0%
ATCCACGGTCCATAATTTGA+CGG + chr2.3:69304568-69304587 None:intergenic 40.0%
ATGAGATTCTTTCGTGCGAT+TGG - chr2.3:69305511-69305530 MS.gene54295:CDS 40.0%
GCATGGTTATGCTGTTAGAA+AGG - chr2.3:69305635-69305654 MS.gene54295:CDS 40.0%
GGAAGAATCATTGTAGAACC+TGG + chr2.3:69305480-69305499 None:intergenic 40.0%
GGGATTGTACTGTCAGAATT+TGG - chr2.3:69304685-69304704 MS.gene54295:intron 40.0%
GTAAAGTTTGACGGAACCAT+CGG + chr2.3:69304421-69304440 None:intergenic 40.0%
TGTAAGGAGTTATAGGGTTC+CGG - chr2.3:69305596-69305615 MS.gene54295:CDS 40.0%
! CAGTTAAGGTTTGGGATGTA+AGG - chr2.3:69305580-69305599 MS.gene54295:CDS 40.0%
! CGGGTTAGAATTGATTATGG+AGG + chr2.3:69305229-69305248 None:intergenic 40.0%
! GAACGAATAGGGTTTGAAGT+AGG + chr2.3:69304459-69304478 None:intergenic 40.0%
! GTTTGGTTCCACTTCTATAC+GGG + chr2.3:69305248-69305267 None:intergenic 40.0%
! TAGGGGTATGTTTGATTGTG+CGG - chr2.3:69304978-69304997 MS.gene54295:intron 40.0%
! TTAAATTTTCGCCGCATGTG+AGG - chr2.3:69305658-69305677 MS.gene54295:CDS 40.0%
!! CATCATACTGAGTTTGCTGT+TGG - chr2.3:69305765-69305784 MS.gene54295:CDS 40.0%
!! GTCGGTTGATAAGTCAGTTA+AGG - chr2.3:69305566-69305585 MS.gene54295:CDS 40.0%
!! TCATACTGAGTTTGCTGTTG+GGG - chr2.3:69305767-69305786 MS.gene54295:CDS 40.0%
!!! ATGTTTTTGCATCGGCTTCT+GGG - chr2.3:69304664-69304683 MS.gene54295:intron 40.0%
AGCGAGGTCGTAAAGTTTGA+CGG + chr2.3:69304430-69304449 None:intergenic 45.0%
ATTTCGGTGATTGGGAGAGT+GGG + chr2.3:69304318-69304337 None:intergenic 45.0%
GCAACATGGAACTGATCCTA+GGG - chr2.3:69305857-69305876 MS.gene54295:CDS 45.0%
GGGACGATACCGTCAAATTA+TGG - chr2.3:69304556-69304575 MS.gene54295:CDS 45.0%
GTAGAAGGGGCTGAATTTGA+CGG + chr2.3:69304211-69304230 None:intergenic 45.0%
TACCGTCAAATTATGGACCG+TGG - chr2.3:69304563-69304582 MS.gene54295:intron 45.0%
TGACGGAGTAACCGTTGAAT+GGG + chr2.3:69304194-69304213 None:intergenic 45.0%
TTGAACGTTCTCACCGAAGT+GGG + chr2.3:69304594-69304613 None:intergenic 45.0%
TTGACGGAGTAACCGTTGAA+TGG + chr2.3:69304195-69304214 None:intergenic 45.0%
TTTGAACGTTCTCACCGAAG+TGG + chr2.3:69304595-69304614 None:intergenic 45.0%
! ATTTGGGATGTTCGTGAACC+AGG - chr2.3:69304702-69304721 MS.gene54295:intron 45.0%
! CGAATAGGGTTTGAAGTAGG+TGG + chr2.3:69304456-69304475 None:intergenic 45.0%
!! GCTTCTTGCTAGTACTGGTT+GGG - chr2.3:69305815-69305834 MS.gene54295:CDS 45.0%
!! TCACGCCGATGTTTTTGCAT+CGG - chr2.3:69304656-69304675 MS.gene54295:intron 45.0%
!!! GATGTTTTTGCATCGGCTTC+TGG - chr2.3:69304663-69304682 MS.gene54295:intron 45.0%
!!! TGTTTTTGCATCGGCTTCTG+GGG - chr2.3:69304665-69304684 MS.gene54295:intron 45.0%
AGAAACGAATCACGGCGAAC+AGG + chr2.3:69304525-69304544 None:intergenic 50.0%
CAGCGGAGAGAAACGAATCA+CGG + chr2.3:69304533-69304552 None:intergenic 50.0%
GAAGGGCTTCTTGCTAGTAC+TGG - chr2.3:69305810-69305829 MS.gene54295:CDS 50.0%
GACGGAGTAACCGTTGAATG+GGG + chr2.3:69304193-69304212 None:intergenic 50.0%
GATTTCGGTGATTGGGAGAG+TGG + chr2.3:69304319-69304338 None:intergenic 50.0%
GCCGCATGTGAGGAATTTGT+TGG - chr2.3:69305668-69305687 MS.gene54295:CDS 50.0%
GCTCAAAACTTCGGCATCCT+CGG - chr2.3:69304255-69304274 MS.gene54295:CDS 50.0%
GGCAACATGGAACTGATCCT+AGG - chr2.3:69305856-69305875 MS.gene54295:CDS 50.0%
TTCCGGTTGCCGTGCTTAAT+GGG - chr2.3:69305613-69305632 MS.gene54295:CDS 50.0%
TTGAAGTAGGTGGAAGAGCG+AGG + chr2.3:69304446-69304465 None:intergenic 50.0%
TTTCGGTGATTGGGAGAGTG+GGG + chr2.3:69304317-69304336 None:intergenic 50.0%
TTTGACGGAACCATCGGCAA+CGG + chr2.3:69304415-69304434 None:intergenic 50.0%
! CGATGCGGTTTTCGTAGAAG+GGG + chr2.3:69304224-69304243 None:intergenic 50.0%
! GCGATGCGGTTTTCGTAGAA+GGG + chr2.3:69304225-69304244 None:intergenic 50.0%
!! GGCTTCTTGCTAGTACTGGT+TGG - chr2.3:69305814-69305833 MS.gene54295:CDS 50.0%
!!! GAGGATGCCGAAGTTTTGAG+CGG + chr2.3:69304256-69304275 None:intergenic 50.0%
AACTTCGGCATCCTCGGCAA+CGG - chr2.3:69304261-69304280 MS.gene54295:CDS 55.0%
ACGGCATCTACGACGTCACA+TGG - chr2.3:69304358-69304377 MS.gene54295:CDS 55.0%
AGAGTTCGAGGACGTGGAGA+CGG + chr2.3:69304287-69304306 None:intergenic 55.0%
AGGCGGCGATTTCGGTGATT+GGG + chr2.3:69304326-69304345 None:intergenic 55.0%
ATTCGTTTCTCTCCGCTGCG+TGG - chr2.3:69304535-69304554 MS.gene54295:CDS 55.0%
GCAAAAACATCGGCGTGACG+AGG + chr2.3:69304654-69304673 None:intergenic 55.0%
GTCGCCACCGCTCAAAACTT+CGG - chr2.3:69304246-69304265 MS.gene54295:CDS 55.0%
GTTCCGGTTGCCGTGCTTAA+TGG - chr2.3:69305612-69305631 MS.gene54295:CDS 55.0%
TAGGCGGCGATTTCGGTGAT+TGG + chr2.3:69304327-69304346 None:intergenic 55.0%
TGCCCATTAAGCACGGCAAC+CGG + chr2.3:69305618-69305637 None:intergenic 55.0%
TGTGACGTCGTAGATGCCGT+CGG + chr2.3:69304358-69304377 None:intergenic 55.0%
TTCGTTTCTCTCCGCTGCGT+GGG - chr2.3:69304536-69304555 MS.gene54295:CDS 55.0%
! GGCGATGCGGTTTTCGTAGA+AGG + chr2.3:69304226-69304245 None:intergenic 55.0%
! GTTGCCGTGCTTAATGGGCA+TGG - chr2.3:69305618-69305637 MS.gene54295:CDS 55.0%
!!! GAAGTTTTGAGCGGTGGCGA+CGG + chr2.3:69304247-69304266 None:intergenic 55.0%
!!! GATGCCGAAGTTTTGAGCGG+TGG + chr2.3:69304253-69304272 None:intergenic 55.0%
CACCGAAGTGGGACGATCCA+CGG + chr2.3:69304583-69304602 None:intergenic 60.0%
GACCGTGGATCGTCCCACTT+CGG - chr2.3:69304578-69304597 MS.gene54295:intron 60.0%
GATTGGGAGAGTGGGGTTGG+AGG + chr2.3:69304310-69304329 None:intergenic 60.0%
GGTGATTGGGAGAGTGGGGT+TGG + chr2.3:69304313-69304332 None:intergenic 60.0%
TGGAGGAGAGTTCGAGGACG+TGG + chr2.3:69304293-69304312 None:intergenic 60.0%
TGGGGTTGGAGGAGAGTTCG+AGG + chr2.3:69304299-69304318 None:intergenic 60.0%
! GGTGTCGTAGGCGGCGATTT+CGG + chr2.3:69304334-69304353 None:intergenic 60.0%
GACGGAACCATCGGCAACGG+CGG + chr2.3:69304412-69304431 None:intergenic 65.0%
GACGGTATCGTCCCACGCAG+CGG + chr2.3:69304550-69304569 None:intergenic 65.0%
GACGTCGTAGATGCCGTCGG+CGG + chr2.3:69304355-69304374 None:intergenic 65.0%
ACCATCGGCAACGGCGGCGA+CGG + chr2.3:69304406-69304425 None:intergenic 70.0%
GGCAACGGCGGCGACGGTAA+TGG + chr2.3:69304400-69304419 None:intergenic 70.0%
!! GATGCCGTCGGCGGTGTCGT+AGG + chr2.3:69304346-69304365 None:intergenic 70.0%
ACCGTCGCCGCCGTTGCCGA+TGG - chr2.3:69304402-69304421 MS.gene54295:CDS 75.0%
CGTGGAGACGGCCGTTGCCG+AGG + chr2.3:69304275-69304294 None:intergenic 75.0%
GAGCGGTGGCGACGGCGATG+CGG + chr2.3:69304239-69304258 None:intergenic 75.0%
GCCGCCTACGACACCGCCGA+CGG - chr2.3:69304339-69304358 MS.gene54295:CDS 75.0%
!! GCCGTCGGCGGTGTCGTAGG+CGG + chr2.3:69304343-69304362 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 69304174 69305887 69304174 ID=MS.gene54295
chr2.3 mRNA 69304174 69305887 69304174 ID=MS.gene54295.t1;Parent=MS.gene54295
chr2.3 exon 69305338 69305887 69305338 ID=MS.gene54295.t1.exon1;Parent=MS.gene54295.t1
chr2.3 CDS 69305338 69305887 69305338 ID=cds.MS.gene54295.t1;Parent=MS.gene54295.t1
chr2.3 exon 69304174 69304580 69304174 ID=MS.gene54295.t1.exon2;Parent=MS.gene54295.t1
chr2.3 CDS 69304174 69304580 69304174 ID=cds.MS.gene54295.t1;Parent=MS.gene54295.t1
Gene Sequence

>MS.gene54295

ATGCCAGTTTTCAAAACCCCATTCAACGGTTACTCCGTCAAATTCAGCCCCTTCTACGAAAACCGCATCGCCGTCGCCACCGCTCAAAACTTCGGCATCCTCGGCAACGGCCGTCTCCACGTCCTCGAACTCTCCTCCAACCCCACTCTCCCAATCACCGAAATCGCCGCCTACGACACCGCCGACGGCATCTACGACGTCACATGGTCTGAATCTCACGACTCCATTACCGTCGCCGCCGTTGCCGATGGTTCCGTCAAACTTTACGACCTCGCTCTTCCACCTACTTCAAACCCTATTCGTTCCTTTCATGAACATACACGTGAAGTTCATTCCGTTGATTATAATCCTGTTCGCCGTGATTCGTTTCTCTCCGCTGCGTGGGACGATACCGTCAAATTATGGACCGTGGATCGTCCCACTTCGGTGAGAACGTTCAAAGAGCATGCTTATTGTGTTTATTCATCTGTTTGGAATCCTCGTCACGCCGATGTTTTTGCATCGGCTTCTGGGGATTGTACTGTCAGAATTTGGGATGTTCGTGAACCAGGTATTACAATTTTGATATAACTCACAAATAAATAACAAATTATACAATGATGCTTATATAGCAAATTAAAACAATTGATTAGTCTAAGTGTATGTTTCATTTTGGAGTGAGAAAAATTGATTTTGTTTAGTAAATTGATTCTGTGAAATTGATTTTAGCTATAGTTAAGTTAAGGTAAAGTGATTTATGTTCATGTAAAAATGAGCTGAACATCGGCAATTTCTAGCTTCAAGTAGAATCCATTAAAGCAAAATTAGGGGTATGTTTGATTGTGCGGTGACAAGAATTGATTTAGTAAAATTGATTGTGACTAAAAGTGAGTTGAAGGTAAAGTGATTTGTGTTTGAATACATTCCCTTAAAAGTTAGTTGAACTATAAATTTGTTTAGAAAAATCAATTCTAGAATCAAAAGCTACAAGTTCTAGCTTCAAGCAGAATCAATTATGGAGGCAAAATCAATAATACTCGTAAGCAACCAAACATATCAAAATCAATTCTAACCCTCCATAATCAATTCTAACCCGTATAGAAGTGGAACCAAACATACACTGATTCTACTTTAGAGGAACCAAAAATGTCAAAATATATTCTACACTTGTAGAATCAATTTTGACTCTTCCAAAAGTGGAACCAAACATACACTAATTTAAGTAACAATTGTGACTAATTATTCTAACTAACTATTACATCCAATACTAGGTTTTTAAGTAATCAGTGTGACTAATTATTCTAACTATCTATTACATCTAATACCAGGTTCTACAATGATTCTTCCGGCACATGAACATGAGATTCTTTCGTGCGATTGGAATAAGTATGATGAATGTATAATTGCAACTGGGTCGGTTGATAAGTCAGTTAAGGTTTGGGATGTAAGGAGTTATAGGGTTCCGGTTGCCGTGCTTAATGGGCATGGTTATGCTGTTAGAAAGGTTAAATTTTCGCCGCATGTGAGGAATTTGTTGGTGTCTTGTTCTTATGATATGACTGTTTGTGTTTGGGATTTTATGGTGGAAGATGCTTTGGTGAGTAGATATGATCATCATACTGAGTTTGCTGTTGGGGTTGATATGAGTGTTCTTGTTGAAGGGCTTCTTGCTAGTACTGGTTGGGATGAACTTGTTTATGTTTGGCAACATGGAACTGATCCTAGGGCATCTTGA
Protein sequence

>MS.gene54295.t1

MPVFKTPFNGYSVKFSPFYENRIAVATAQNFGILGNGRLHVLELSSNPTLPITEIAAYDTADGIYDVTWSESHDSITVAAVADGSVKLYDLALPPTSNPIRSFHEHTREVHSVDYNPVRRDSFLSAAWDDTVKLWTVDRPTSVRTFKEHAYCVYSSVWNPRHADVFASASGDCTVRIWDVREPGSTMILPAHEHEILSCDWNKYDECIIATGSVDKSVKVWDVRSYRVPVAVLNGHGYAVRKVKFSPHVRNLLVSCSYDMTVCVWDFMVEDALVSRYDHHTEFAVGVDMSVLVEGLLASTGWDELVYVWQHGTDPRAS