Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55291.t1 | XP_003613911.1 | 91.9 | 259 | 19 | 1 | 268 | 524 | 7 | 265 | 4.10E-126 | 461.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55291.t1 | P55852 | 91.9 | 37 | 3 | 0 | 154 | 190 | 58 | 94 | 9.9e-12 | 73.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55291.t1 | G7JWJ7 | 91.9 | 259 | 19 | 1 | 268 | 524 | 7 | 265 | 3.0e-126 | 461.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050170 | MS.gene55291 | 0.807541 | 4.81E-50 | -1.69E-46 |
| MS.gene052100 | MS.gene55291 | 0.804398 | 2.20E-49 | -1.69E-46 |
| MS.gene055748 | MS.gene55291 | 0.817448 | 3.28E-52 | -1.69E-46 |
| MS.gene055750 | MS.gene55291 | 0.819822 | 9.49E-53 | -1.69E-46 |
| MS.gene055751 | MS.gene55291 | 0.820923 | 5.31E-53 | -1.69E-46 |
| MS.gene05589 | MS.gene55291 | 0.828377 | 9.30E-55 | -1.69E-46 |
| MS.gene056266 | MS.gene55291 | 0.823716 | 1.19E-53 | -1.69E-46 |
| MS.gene056283 | MS.gene55291 | 0.81401 | 1.92E-51 | -1.69E-46 |
| MS.gene056387 | MS.gene55291 | 0.812078 | 5.08E-51 | -1.69E-46 |
| MS.gene06000 | MS.gene55291 | 0.807083 | 6.01E-50 | -1.69E-46 |
| MS.gene060982 | MS.gene55291 | 0.817026 | 4.08E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55291.t1 | MTR_5g042520 | 93.436 | 259 | 15 | 1 | 268 | 524 | 7 | 265 | 5.45e-174 | 491 |
| MS.gene55291.t1 | MTR_5g042520 | 91.398 | 186 | 14 | 1 | 268 | 451 | 7 | 192 | 1.29e-115 | 343 |
| MS.gene55291.t1 | MTR_5g044170 | 87.500 | 128 | 16 | 0 | 1 | 128 | 212 | 339 | 7.07e-71 | 231 |
| MS.gene55291.t1 | MTR_1g108470 | 37.600 | 125 | 74 | 2 | 1 | 125 | 201 | 321 | 2.89e-22 | 98.6 |
| MS.gene55291.t1 | MTR_3g053540 | 86.047 | 43 | 6 | 0 | 149 | 191 | 58 | 100 | 2.26e-18 | 80.9 |
| MS.gene55291.t1 | MTR_3g053520 | 86.047 | 43 | 6 | 0 | 149 | 191 | 58 | 100 | 3.31e-18 | 80.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55291.t1 | AT1G13990 | 72.308 | 195 | 45 | 2 | 331 | 522 | 87 | 275 | 2.81e-94 | 288 |
| MS.gene55291.t1 | AT1G13990 | 62.780 | 223 | 46 | 3 | 331 | 522 | 87 | 303 | 4.31e-87 | 271 |
| MS.gene55291.t1 | AT1G13990 | 55.459 | 229 | 56 | 8 | 331 | 522 | 87 | 306 | 7.10e-64 | 211 |
| MS.gene55291.t1 | AT5G55160 | 87.805 | 41 | 5 | 0 | 149 | 189 | 52 | 92 | 2.27e-17 | 78.2 |
| MS.gene55291.t1 | AT5G55160 | 85.714 | 42 | 6 | 0 | 149 | 190 | 65 | 106 | 9.96e-17 | 76.6 |
| MS.gene55291.t1 | AT4G26840 | 84.615 | 39 | 6 | 0 | 149 | 187 | 53 | 91 | 3.24e-15 | 72.0 |
Find 114 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAGATTGCAGTCAGGTTT+TGG | 0.305333 | 5.2:-31906823 | MS.gene55291:CDS |
| CGACGGAAGGGGTTGGTTGG+AGG | 0.305348 | 5.2:+31902910 | None:intergenic |
| TGATATGCTCCTCTATTATC+TGG | 0.320298 | 5.2:-31906795 | MS.gene55291:CDS |
| CGGCTTTGTATGTCAGTGAT+TGG | 0.322440 | 5.2:-31907366 | MS.gene55291:CDS |
| TGGAATCAGAGATATTCTTT+TGG | 0.334932 | 5.2:-31907332 | MS.gene55291:CDS |
| AAGAACTCATCCGAGCTTCC+AGG | 0.336242 | 5.2:+31902715 | None:intergenic |
| GAGCTCGAAGATTAAGCTTA+AGG | 0.349622 | 5.2:+31899136 | None:intergenic |
| TAGAGGCAGGAACGTTGATT+TGG | 0.353301 | 5.2:-31907477 | MS.gene55291:CDS |
| AGTGATTGGCTTGTTGTTCA+TGG | 0.359249 | 5.2:-31907352 | MS.gene55291:CDS |
| TTTCTTGATTTACAGTTTCT+CGG | 0.363406 | 5.2:-31907646 | MS.gene55291:CDS |
| CGATATTTCGTTCCTCCTCC+TGG | 0.379040 | 5.2:+31902816 | None:intergenic |
| CATATGGATGGGAAAATTCC+TGG | 0.384444 | 5.2:-31902733 | MS.gene55291:CDS |
| AATCGCACCTTATATTTCTC+CGG | 0.386676 | 5.2:+31899994 | None:intergenic |
| GCATGATCTGGTTACGTTAG+AGG | 0.391529 | 5.2:-31907494 | MS.gene55291:CDS |
| TCCTTCAGTCTCACCTTCCA+TGG | 0.402293 | 5.2:-31902938 | MS.gene55291:intron |
| TGAAAACCCGGAGAAATATA+AGG | 0.409559 | 5.2:-31900001 | MS.gene55291:intron |
| TCAGATCATCTTGATCTTCA+AGG | 0.424973 | 5.2:+31899103 | None:intergenic |
| CTAATATCTTCTACTTGCAT+TGG | 0.426258 | 5.2:+31899798 | None:intergenic |
| TTTCGTTCCTCCTCCTGGTT+CGG | 0.427631 | 5.2:+31902821 | None:intergenic |
| TTGATCTTTGCAAGAAAATG+AGG | 0.433526 | 5.2:-31899209 | MS.gene55291:CDS |
| GGCTTTGAAGTTTCTCTCCA+CGG | 0.442555 | 5.2:+31900065 | None:intergenic |
| TCGGCGGGTGTGGCGACGGA+AGG | 0.445087 | 5.2:+31902897 | None:intergenic |
| GGCGGGTGTGGCGACGGAAG+GGG | 0.448457 | 5.2:+31902899 | None:intergenic |
| AGAGAGAAAGTCGGCGGGTG+TGG | 0.449002 | 5.2:+31902887 | None:intergenic |
| AGCAGCGGTGGAGGAGAAGA+CGG | 0.453767 | 5.2:-31902784 | MS.gene55291:CDS |
| TACCGATGAGGCGTCTATCT+CGG | 0.460916 | 5.2:+31899320 | None:intergenic |
| TGCATATTGACTTGCGTCAA+TGG | 0.461133 | 5.2:-31906752 | MS.gene55291:CDS |
| TCTTCCATCTCCAACTCATC+AGG | 0.462279 | 5.2:+31907036 | None:intergenic |
| TTAATCTTCGAGCTCGAAAA+CGG | 0.465903 | 5.2:-31899128 | MS.gene55291:CDS |
| GGTGGAGAAAGCGCTTCATA+TGG | 0.468825 | 5.2:-31902749 | MS.gene55291:CDS |
| CACTCCGATCGACGAAGCTA+TGG | 0.474935 | 5.2:-31907590 | MS.gene55291:CDS |
| TTTGATTCTAGCAGCTATGA+TGG | 0.481586 | 5.2:-31902640 | MS.gene55291:intron |
| ATCGGAGTGAAAACAATTAA+CGG | 0.482963 | 5.2:+31907604 | None:intergenic |
| TAAGATTGTGTGGCATGATC+TGG | 0.483463 | 5.2:-31907506 | MS.gene55291:CDS |
| CGGCGGGTGTGGCGACGGAA+GGG | 0.487980 | 5.2:+31902898 | None:intergenic |
| TGCAAAGTCAGCAGCGGTGG+AGG | 0.488867 | 5.2:-31902793 | MS.gene55291:CDS |
| CAAGTAGAAGATATTAGAGA+TGG | 0.493706 | 5.2:-31899792 | MS.gene55291:CDS |
| GGAAGGGGTTGGTTGGAGGG+AGG | 0.497303 | 5.2:+31902914 | None:intergenic |
| TGGCGACGGAAGGGGTTGGT+TGG | 0.498052 | 5.2:+31902907 | None:intergenic |
| AGAAGAGTCTCGGAGGAAGA+TGG | 0.501382 | 5.2:+31902857 | None:intergenic |
| GCAGCGGTGGAGGAGAAGAC+GGG | 0.505410 | 5.2:-31902783 | MS.gene55291:CDS |
| GACGGAAGGGGTTGGTTGGA+GGG | 0.509870 | 5.2:+31902911 | None:intergenic |
| AGTGAAGTTGAAAGAGAGCT+TGG | 0.510848 | 5.2:-31899900 | MS.gene55291:CDS |
| AAAGTCGGCGGGTGTGGCGA+CGG | 0.517140 | 5.2:+31902893 | None:intergenic |
| GACTATGATAAGCTTGCTGC+AGG | 0.517372 | 5.2:-31906854 | MS.gene55291:CDS |
| ACTCATAAACCAGATAATAG+AGG | 0.519980 | 5.2:+31906786 | None:intergenic |
| AAATCAGTCTAAACAAGCAA+GGG | 0.520422 | 5.2:-31899835 | MS.gene55291:CDS |
| AGAAGACGGGAGAGATGAAG+AGG | 0.520462 | 5.2:-31902770 | MS.gene55291:CDS |
| CTACACATCGCGTCCGAACC+AGG | 0.520972 | 5.2:-31902834 | MS.gene55291:CDS |
| CAAGTTGTGTTTGCACTAAG+TGG | 0.523402 | 5.2:-31906724 | MS.gene55291:intron |
| ATCGCACCTTATATTTCTCC+GGG | 0.524178 | 5.2:+31899995 | None:intergenic |
| AGATATTAGAGATGGAGTGC+TGG | 0.526832 | 5.2:-31899784 | MS.gene55291:intron |
| GATGAGTTGGAGATGGAAGA+TGG | 0.527563 | 5.2:-31907033 | MS.gene55291:CDS |
| GGTGTGGCGACGGAAGGGGT+TGG | 0.531215 | 5.2:+31902903 | None:intergenic |
| AGACGGGAGAGATGAAGAGG+TGG | 0.531364 | 5.2:-31902767 | MS.gene55291:CDS |
| GCTTTGAAGTTTCTCTCCAC+GGG | 0.532223 | 5.2:+31900066 | None:intergenic |
| CTCACCATAGCTTCGTCGAT+CGG | 0.532818 | 5.2:+31907586 | None:intergenic |
| GAGAAAGCGCTTCATATGGA+TGG | 0.539172 | 5.2:-31902745 | MS.gene55291:CDS |
| GAGTCTCGGAGGAAGATGGC+GGG | 0.543744 | 5.2:+31902861 | None:intergenic |
| GACTCCTGATGAGTTGGAGA+TGG | 0.544981 | 5.2:-31907040 | MS.gene55291:CDS |
| TGATCTTTGCAAGAAAATGA+GGG | 0.547590 | 5.2:-31899208 | MS.gene55291:CDS |
| AAGATGACGGCAGATTATAT+CGG | 0.550913 | 5.2:+31907537 | None:intergenic |
| AGTGTATGTACTCACCCAAA+AGG | 0.551076 | 5.2:-31900042 | MS.gene55291:CDS |
| TTGCAGTTCTATTCCGCAGA+GGG | 0.551682 | 5.2:-31899182 | MS.gene55291:CDS |
| ATCATAGCTGCTAGAATCAA+AGG | 0.555592 | 5.2:+31902642 | None:intergenic |
| GACGCCATGCTTCATCAGAC+AGG | 0.556706 | 5.2:-31907000 | MS.gene55291:CDS |
| AAAATCAGTCTAAACAAGCA+AGG | 0.561023 | 5.2:-31899836 | MS.gene55291:CDS |
| TGGGAAAATTCCTGGAAGCT+CGG | 0.562065 | 5.2:-31902725 | MS.gene55291:CDS |
| CAGATCATCTTGATCTTCAA+GGG | 0.562330 | 5.2:+31899104 | None:intergenic |
| GGTATTTGAAGATGAAAATG+AGG | 0.563344 | 5.2:-31899388 | MS.gene55291:CDS |
| ATGAGTTGGAGATGGAAGAT+GGG | 0.564934 | 5.2:-31907032 | MS.gene55291:CDS |
| GATCTGGTTACGTTAGAGGC+AGG | 0.574338 | 5.2:-31907490 | MS.gene55291:CDS |
| TGCCGAGATAGACGCCTCAT+CGG | 0.575782 | 5.2:-31899322 | MS.gene55291:intron |
| TCGTGCAAAGTCAGCAGCGG+TGG | 0.577748 | 5.2:-31902796 | MS.gene55291:CDS |
| TGAACAGACTCCTGATGAGT+TGG | 0.578267 | 5.2:-31907046 | MS.gene55291:CDS |
| ACCTCCTGTCTGATGAAGCA+TGG | 0.580486 | 5.2:+31906996 | None:intergenic |
| ATCGCGTCCGAACCAGGAGG+AGG | 0.582438 | 5.2:-31902828 | MS.gene55291:CDS |
| CTTGCTGCAAGGAGGTTGTG+AGG | 0.590015 | 5.2:-31899349 | MS.gene55291:CDS |
| AGTCTAAACAAGCAAGGGGA+GGG | 0.591602 | 5.2:-31899830 | MS.gene55291:CDS |
| TTGCTGCAAGGAGGTTGTGA+GGG | 0.596232 | 5.2:-31899348 | MS.gene55291:CDS |
| AGAAAGCGCTTCATATGGAT+GGG | 0.602606 | 5.2:-31902744 | MS.gene55291:CDS |
| TTGAATGTGAAAACTAGTGC+TGG | 0.604966 | 5.2:-31907433 | MS.gene55291:CDS |
| CAGTCTAAACAAGCAAGGGG+AGG | 0.606675 | 5.2:-31899831 | MS.gene55291:CDS |
| AGAGTCTCGGAGGAAGATGG+CGG | 0.608815 | 5.2:+31902860 | None:intergenic |
| GTTGGTTGGAGGGAGGACCA+TGG | 0.609914 | 5.2:+31902921 | None:intergenic |
| TTTGGTAATTTGTACCGATG+AGG | 0.610367 | 5.2:+31899308 | None:intergenic |
| AATTGTGCACGATGAAAACC+CGG | 0.610498 | 5.2:-31900013 | MS.gene55291:CDS |
| TACATCAGGTTCTCTTCAAG+TGG | 0.612786 | 5.2:-31906891 | MS.gene55291:intron |
| CAGTTCTATTCCGCAGAGGG+AGG | 0.613903 | 5.2:-31899179 | MS.gene55291:CDS |
| TCCTACGCATATCATAAGCA+CGG | 0.615988 | 5.2:+31902677 | None:intergenic |
| GGAGAAGAGAGAAAGTCGGC+GGG | 0.619041 | 5.2:+31902882 | None:intergenic |
| AAGTATTGTGACTTGCTGCA+AGG | 0.620712 | 5.2:-31899360 | MS.gene55291:CDS |
| CCTACGCATATCATAAGCAC+GGG | 0.621176 | 5.2:+31902678 | None:intergenic |
| GTTGGAGGGAGGACCATGGA+AGG | 0.624074 | 5.2:+31902925 | None:intergenic |
| GGCGGGAGAAGAGAGAAAGT+CGG | 0.624435 | 5.2:+31902878 | None:intergenic |
| GCCATGCTTCATCAGACAGG+AGG | 0.629302 | 5.2:-31906997 | MS.gene55291:intron |
| GAAGCTATGGTGAGTCACGT+CGG | 0.629812 | 5.2:-31907577 | MS.gene55291:CDS |
| TCTTACACGGCACAAGATGA+CGG | 0.631306 | 5.2:+31907524 | None:intergenic |
| TTGTGCCGTGTAAGATTGTG+TGG | 0.636967 | 5.2:-31907516 | MS.gene55291:CDS |
| CTTGCAGTTCTATTCCGCAG+AGG | 0.639139 | 5.2:-31899183 | MS.gene55291:CDS |
| CGATGTGTAGAGAAGAGTCT+CGG | 0.639811 | 5.2:+31902847 | None:intergenic |
| CCCGTGCTTATGATATGCGT+AGG | 0.646813 | 5.2:-31902678 | MS.gene55291:CDS |
| GATCATCATCATCACTGTCA+CGG | 0.647986 | 5.2:-31907245 | MS.gene55291:CDS |
| TCATGCCACACAATCTTACA+CGG | 0.648152 | 5.2:+31907511 | None:intergenic |
| GAGTCACGTCGGAAACGTGA+CGG | 0.651959 | 5.2:-31907566 | MS.gene55291:CDS |
| ACGCATATCATAAGCACGGG+AGG | 0.665509 | 5.2:+31902681 | None:intergenic |
| CAGGAGGTGTCCTCCCTCTG+CGG | 0.666929 | 5.2:+31899169 | None:intergenic |
| GGGAGAAGAGAGAAAGTCGG+CGG | 0.667555 | 5.2:+31902881 | None:intergenic |
| TGAGTTGGAGATGGAAGATG+GGG | 0.668703 | 5.2:-31907031 | MS.gene55291:CDS |
| AATCAGTCTAAACAAGCAAG+GGG | 0.668776 | 5.2:-31899834 | MS.gene55291:CDS |
| TGTGTAGAGAAGAGTCTCGG+AGG | 0.689941 | 5.2:+31902850 | None:intergenic |
| ATATCGTGCAAAGTCAGCAG+CGG | 0.717527 | 5.2:-31902799 | MS.gene55291:CDS |
| TATTGTGACTTGCTGCAAGG+AGG | 0.750982 | 5.2:-31899357 | MS.gene55291:CDS |
| CACATCGCGTCCGAACCAGG+AGG | 0.764506 | 5.2:-31902831 | MS.gene55291:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 31899098 | 31907677 | 31899098 | ID=MS.gene55291 |
| chr5.2 | mRNA | 31899098 | 31907677 | 31899098 | ID=MS.gene55291.t1;Parent=MS.gene55291 |
| chr5.2 | exon | 31907225 | 31907677 | 31907225 | ID=MS.gene55291.t1.exon1;Parent=MS.gene55291.t1 |
| chr5.2 | CDS | 31907225 | 31907677 | 31907225 | ID=cds.MS.gene55291.t1;Parent=MS.gene55291.t1 |
| chr5.2 | exon | 31906998 | 31907109 | 31906998 | ID=MS.gene55291.t1.exon2;Parent=MS.gene55291.t1 |
| chr5.2 | CDS | 31906998 | 31907109 | 31906998 | ID=cds.MS.gene55291.t1;Parent=MS.gene55291.t1 |
| chr5.2 | exon | 31906725 | 31906905 | 31906725 | ID=MS.gene55291.t1.exon3;Parent=MS.gene55291.t1 |
| chr5.2 | CDS | 31906725 | 31906905 | 31906725 | ID=cds.MS.gene55291.t1;Parent=MS.gene55291.t1 |
| chr5.2 | exon | 31904883 | 31904938 | 31904883 | ID=MS.gene55291.t1.exon4;Parent=MS.gene55291.t1 |
| chr5.2 | CDS | 31904883 | 31904938 | 31904883 | ID=cds.MS.gene55291.t1;Parent=MS.gene55291.t1 |
| chr5.2 | exon | 31902641 | 31902952 | 31902641 | ID=MS.gene55291.t1.exon5;Parent=MS.gene55291.t1 |
| chr5.2 | CDS | 31902641 | 31902952 | 31902641 | ID=cds.MS.gene55291.t1;Parent=MS.gene55291.t1 |
| chr5.2 | exon | 31900002 | 31900101 | 31900002 | ID=MS.gene55291.t1.exon6;Parent=MS.gene55291.t1 |
| chr5.2 | CDS | 31900002 | 31900101 | 31900002 | ID=cds.MS.gene55291.t1;Parent=MS.gene55291.t1 |
| chr5.2 | exon | 31899785 | 31899920 | 31899785 | ID=MS.gene55291.t1.exon7;Parent=MS.gene55291.t1 |
| chr5.2 | CDS | 31899785 | 31899920 | 31899785 | ID=cds.MS.gene55291.t1;Parent=MS.gene55291.t1 |
| chr5.2 | exon | 31899323 | 31899409 | 31899323 | ID=MS.gene55291.t1.exon8;Parent=MS.gene55291.t1 |
| chr5.2 | CDS | 31899323 | 31899409 | 31899323 | ID=cds.MS.gene55291.t1;Parent=MS.gene55291.t1 |
| chr5.2 | exon | 31899098 | 31899235 | 31899098 | ID=MS.gene55291.t1.exon9;Parent=MS.gene55291.t1 |
| chr5.2 | CDS | 31899098 | 31899235 | 31899098 | ID=cds.MS.gene55291.t1;Parent=MS.gene55291.t1 |
| Gene Sequence |
| Protein sequence |
