AlfalfaGEDB | MS.gene55561

M. sativa cultivar XinJiangDaYe / MS.gene55561

KEGG: K14432
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55561.t1	XP_003618910.1	91.2	228	10	2	1	219	1	227	2.20E-105	391.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55561.t1	Q9LES3	50.5	222	86	6	1	218	1	202	8.2e-40	165.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55561.t1	G7KP64	91.2	228	10	2	1	219	1	227	1.6e-105	391.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene059171	MS.gene55561	0.805005	1.64E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene55561	MS.gene013906	PPI
MS.gene55561	MS.gene41866	PPI
MS.gene55561	MS.gene068056	PPI
MS.gene55561	MS.gene47208	PPI
MS.gene55561	MS.gene62322	PPI
MS.gene62322	MS.gene55561	PPI
MS.gene013906	MS.gene55561	PPI
MS.gene41906	MS.gene55561	PPI
MS.gene55561	MS.gene83996	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55561.t1	MTR_6g026800	91.228	228	10	2	1	219	1	227	1.08e-141	399
MS.gene55561.t1	MTR_7g088090	55.983	234	82	9	1	220	1	227	4.72e-65	204
MS.gene55561.t1	MTR_8g043960	48.663	187	85	5	16	199	24	202	1.33e-45	154
MS.gene55561.t1	MTR_3g010660	44.860	214	99	7	1	203	1	206	4.47e-43	147
MS.gene55561.t1	MTR_3g101780	50.735	136	62	3	2	137	14	144	2.39e-32	122
MS.gene55561.t1	MTR_4g085910	42.361	144	72	2	16	153	18	156	1.77e-27	107
MS.gene55561.t1	MTR_5g088230	43.791	153	83	3	1	151	6	157	2.62e-24	97.4
MS.gene55561.t1	MTR_5g088230	46.377	138	71	3	1	136	1	137	2.76e-24	97.4
MS.gene55561.t1	MTR_7g104480	38.732	142	61	3	22	141	45	182	1.24e-21	92.4
MS.gene55561.t1	MTR_2g086340	38.462	130	69	4	17	138	23	149	1.87e-17	79.7
MS.gene55561.t1	MTR_2g086390	37.500	128	68	4	20	138	12	136	2.59e-14	70.5
MS.gene55561.t1	MTR_2g086390	37.500	128	68	4	20	138	12	136	3.28e-14	70.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55561.t1	AT3G56850	50.450	222	86	6	1	218	1	202	4.13e-53	173
MS.gene55561.t1	AT2G41070	48.045	179	71	4	1	175	1	161	2.63e-39	136
MS.gene55561.t1	AT2G41070	48.045	179	71	4	1	175	10	170	4.94e-39	136
MS.gene55561.t1	AT2G41070	48.045	179	71	4	1	175	1	161	5.05e-39	135
MS.gene55561.t1	AT2G41070	48.045	179	71	4	1	175	1	161	5.05e-39	135
MS.gene55561.t1	AT2G41070	48.045	179	71	4	1	175	1	161	5.05e-39	135
MS.gene55561.t1	AT1G49720	47.945	146	59	3	5	137	12	153	2.93e-30	115
MS.gene55561.t1	AT1G49720	47.945	146	59	3	5	137	12	153	3.51e-30	115
MS.gene55561.t1	AT1G49720	47.945	146	59	3	5	137	12	153	3.51e-30	115
MS.gene55561.t1	AT3G19290	43.662	142	62	2	14	137	32	173	1.55e-27	108
MS.gene55561.t1	AT3G19290	43.662	142	62	2	14	137	32	173	2.35e-27	108
MS.gene55561.t1	AT3G19290	43.662	142	62	2	14	137	32	173	2.55e-27	108
MS.gene55561.t1	AT3G19290	43.662	142	62	2	14	137	32	173	2.55e-27	108
MS.gene55561.t1	AT3G19290	43.662	142	62	2	14	137	32	173	2.60e-27	108
MS.gene55561.t1	AT1G45249	45.588	136	57	4	16	137	21	153	7.15e-22	92.8
MS.gene55561.t1	AT1G45249	45.588	136	57	4	16	137	21	153	7.15e-22	92.8
MS.gene55561.t1	AT1G45249	45.588	136	57	4	16	137	21	153	8.29e-22	92.8
MS.gene55561.t1	AT1G45249	45.588	136	57	4	16	137	21	153	8.29e-22	92.8
MS.gene55561.t1	AT1G45249	45.588	136	57	4	16	137	21	153	8.29e-22	92.8
MS.gene55561.t1	AT1G45249	45.588	136	57	4	16	137	21	153	8.29e-22	92.8
MS.gene55561.t1	AT1G45249	45.588	136	57	4	16	137	36	168	8.90e-22	92.8
MS.gene55561.t1	AT1G45249	45.588	136	57	4	16	137	36	168	8.90e-22	92.8
MS.gene55561.t1	AT1G45249	45.588	136	57	4	16	137	21	153	9.03e-22	92.8
MS.gene55561.t1	AT1G45249	45.588	136	57	4	16	137	21	153	9.68e-22	92.8
MS.gene55561.t1	AT4G34000	38.415	164	62	5	13	137	24	187	4.61e-21	90.9
MS.gene55561.t1	AT4G34000	38.415	164	62	5	13	137	24	187	7.31e-21	90.5
MS.gene55561.t1	AT4G34000	38.415	164	62	5	13	137	24	187	7.31e-21	90.5
MS.gene55561.t1	AT4G34000	38.415	164	62	5	13	137	24	187	7.31e-21	90.5
MS.gene55561.t1	AT3G44460	42.754	138	62	4	11	137	30	161	1.05e-20	89.0
MS.gene55561.t1	AT2G36270	33.880	183	60	4	16	137	37	219	3.45e-17	80.1
MS.gene55561.t1	AT2G36270	33.880	183	60	4	16	137	37	219	3.45e-17	80.1
MS.gene55561.t1	AT2G36270	33.880	183	60	4	16	137	37	219	5.00e-17	79.3
MS.gene55561.t1	AT5G42910	38.400	125	57	3	16	136	20	128	2.36e-13	68.6

Find 52 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAAAACCATCTTGGTAATTT+AGG	0.046059	6.2:-18144102	MS.gene55561:CDS
CAAGGGAGTTTAACTTTATC+TGG	0.185884	6.2:-18143958	MS.gene55561:CDS
GATATCGCCATCTTCTTTAA+TGG	0.187061	6.2:-18143563	MS.gene55561:CDS
AAAACCATCTTGGTAATTTA+GGG	0.238621	6.2:-18144101	MS.gene55561:CDS
GCTGGCGAGGTTTCGGATTT+CGG	0.294514	6.2:-18144021	MS.gene55561:CDS
GATGACTTTGGAGGATTTCT+TGG	0.296873	6.2:-18143827	MS.gene55561:CDS
TGTTGAAGCTGGCGAGGTTT+CGG	0.333824	6.2:-18144028	MS.gene55561:CDS
GAAAGAAGAGGGGTGTTGAT+AGG	0.340131	6.2:-18143888	MS.gene55561:CDS
TTTCACAACATGGTCTTCAT+TGG	0.341391	6.2:-18143717	MS.gene55561:CDS
GGTTGCCGGGAATCAAGTTT+TGG	0.343807	6.2:-18143593	MS.gene55561:CDS
GTAAGGAAAATGGTTTGTTA+AGG	0.350813	6.2:-18143771	MS.gene55561:CDS
ATATCGCCATCTTCTTTAAT+GGG	0.357518	6.2:-18143562	MS.gene55561:CDS
CAAAGACTTTACCTTTATCT+AGG	0.373965	6.2:-18144161	MS.gene55561:CDS
TAAGGAAAATGGTTTGTTAA+GGG	0.386565	6.2:-18143770	MS.gene55561:CDS
AGTGATGTTGCTGCTGCTGC+TGG	0.393090	6.2:-18143994	MS.gene55561:CDS
TGCATCCAAAACTTGATTCC+CGG	0.395693	6.2:+18143588	None:intergenic
GAGGATTTCTTGGTGAAAGC+TGG	0.421412	6.2:-18143817	MS.gene55561:CDS
ACTTTACCTTTATCTAGGTC+AGG	0.424724	6.2:-18144156	MS.gene55561:CDS
AAAGATCCTGACCTAGATAA+AGG	0.429936	6.2:+18144150	None:intergenic
TGATTCCCGGCAACCTGAAA+AGG	0.430801	6.2:+18143601	None:intergenic
AAAGAAGAGGGGTGTTGATA+GGG	0.431743	6.2:-18143887	MS.gene55561:CDS
GATGAAGTTCAAAACCATCT+TGG	0.441683	6.2:-18144111	MS.gene55561:CDS
ATGAGTTGCTTAAGAGTGTA+TGG	0.441846	6.2:-18144053	MS.gene55561:CDS
TTGGTGAAAGCTGGAGTTGT+TGG	0.447659	6.2:-18143808	MS.gene55561:CDS
TTGATTCGAATGAGGATTCT+CGG	0.469255	6.2:-18143747	MS.gene55561:CDS
TCTTCATTGGATGCAATATC+CGG	0.491078	6.2:-18143704	MS.gene55561:CDS
GTTTCCCTAAATTACCAAGA+TGG	0.502080	6.2:+18144097	None:intergenic
GACACTTGGTGAGATGACTT+TGG	0.506850	6.2:-18143839	MS.gene55561:CDS
AGAGGGGTGTTGATAGGGAT+AGG	0.509576	6.2:-18143882	MS.gene55561:CDS
GTAGTACCCATTAAAGAAGA+TGG	0.510152	6.2:+18143556	None:intergenic
GCATGAACTGCCGCAAAACC+CGG	0.510299	6.2:+18143634	None:intergenic
GTATGAAAAGCATATGATGC+CGG	0.511392	6.2:-18143653	MS.gene55561:CDS
GTTAAGGGTTGATTCGAATG+AGG	0.514656	6.2:-18143755	MS.gene55561:CDS
ACTTGGTGAGATGACTTTGG+AGG	0.526942	6.2:-18143836	MS.gene55561:CDS
AACCTGAAAAGGCTGTTGAA+TGG	0.537317	6.2:+18143612	None:intergenic
CGGCAAAATGTTTCACAACA+TGG	0.538567	6.2:-18143727	MS.gene55561:CDS
AGATATGCAAGGAAAGAAGA+GGG	0.552675	6.2:-18143899	MS.gene55561:CDS
TATGAAAAGCATATGATGCC+GGG	0.578298	6.2:-18143652	MS.gene55561:CDS
GTTGTTGCTGCTGAACAGAA+TGG	0.584868	6.2:+18143680	None:intergenic
AAGATATGCAAGGAAAGAAG+AGG	0.592702	6.2:-18143900	MS.gene55561:CDS
GCTGGTGCTTTGAATTCACA+AGG	0.595492	6.2:-18143976	MS.gene55561:CDS
GGTAATTTAGGGAAACCACT+TGG	0.595722	6.2:-18144090	MS.gene55561:CDS
AGTGTATGGAGTGTTGAAGC+TGG	0.601387	6.2:-18144039	MS.gene55561:CDS
TAGTAAGAAAACTGTTGATG+AGG	0.605589	6.2:-18143929	MS.gene55561:CDS
AGAAAACTGTTGATGAGGTG+TGG	0.606026	6.2:-18143924	MS.gene55561:CDS
TGCTGCTGAACAGAATGGAC+CGG	0.626230	6.2:+18143685	None:intergenic
ATGGAGTGTTGAAGCTGGCG+AGG	0.647172	6.2:-18144034	MS.gene55561:CDS
TCAAGATTCATACTTCCAAG+TGG	0.648117	6.2:+18144075	None:intergenic
AAAGTCATCTCACCAAGTGT+CGG	0.651142	6.2:+18143841	None:intergenic
GATATGCAAGGAAAGAAGAG+GGG	0.657817	6.2:-18143898	MS.gene55561:CDS
CTGGTGCTTTGAATTCACAA+GGG	0.701100	6.2:-18143975	MS.gene55561:CDS
GAGGTGTGGAAAGATATGCA+AGG	0.778137	6.2:-18143910	MS.gene55561:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAACCATCTTGGTAATTTA+GGG	-	chr6.2:18143634-18143653	MS.gene55561:CDS	25.0%
!!!	TAAGGAAAATGGTTTGTTAA+GGG	-	chr6.2:18143965-18143984	MS.gene55561:CDS	25.0%
	ATATCGCCATCTTCTTTAAT+GGG	-	chr6.2:18144173-18144192	MS.gene55561:CDS	30.0%
	CAAAGACTTTACCTTTATCT+AGG	-	chr6.2:18143574-18143593	MS.gene55561:CDS	30.0%
	TAGTAAGAAAACTGTTGATG+AGG	-	chr6.2:18143806-18143825	MS.gene55561:CDS	30.0%
!	CAAAACCATCTTGGTAATTT+AGG	-	chr6.2:18143633-18143652	MS.gene55561:CDS	30.0%
!	TCTTTTCATGGTAAGGAAAA+TGG	-	chr6.2:18143954-18143973	MS.gene55561:CDS	30.0%
!!	GTAAGGAAAATGGTTTGTTA+AGG	-	chr6.2:18143964-18143983	MS.gene55561:CDS	30.0%
	AAAGATCCTGACCTAGATAA+AGG	+	chr6.2:18143588-18143607	None:intergenic	35.0%
	AAGATATGCAAGGAAAGAAG+AGG	-	chr6.2:18143835-18143854	MS.gene55561:CDS	35.0%
	ACTTTACCTTTATCTAGGTC+AGG	-	chr6.2:18143579-18143598	MS.gene55561:CDS	35.0%
	AGATATGCAAGGAAAGAAGA+GGG	-	chr6.2:18143836-18143855	MS.gene55561:CDS	35.0%
	GATATCGCCATCTTCTTTAA+TGG	-	chr6.2:18144172-18144191	MS.gene55561:CDS	35.0%
	GATGAAGTTCAAAACCATCT+TGG	-	chr6.2:18143624-18143643	MS.gene55561:CDS	35.0%
	GTAGTACCCATTAAAGAAGA+TGG	+	chr6.2:18144182-18144201	None:intergenic	35.0%
	GTTTCCCTAAATTACCAAGA+TGG	+	chr6.2:18143641-18143660	None:intergenic	35.0%
	TCAAGATTCATACTTCCAAG+TGG	+	chr6.2:18143663-18143682	None:intergenic	35.0%
	TCTTCATTGGATGCAATATC+CGG	-	chr6.2:18144031-18144050	MS.gene55561:CDS	35.0%
	TTTCACAACATGGTCTTCAT+TGG	-	chr6.2:18144018-18144037	MS.gene55561:CDS	35.0%
!	ATGAGTTGCTTAAGAGTGTA+TGG	-	chr6.2:18143682-18143701	MS.gene55561:CDS	35.0%
!	CAAGGGAGTTTAACTTTATC+TGG	-	chr6.2:18143777-18143796	MS.gene55561:CDS	35.0%
!	GTATGAAAAGCATATGATGC+CGG	-	chr6.2:18144082-18144101	MS.gene55561:CDS	35.0%
!	TATGAAAAGCATATGATGCC+GGG	-	chr6.2:18144083-18144102	MS.gene55561:CDS	35.0%
!	TGGTGAATCTTTTCATGGTA+AGG	-	chr6.2:18143947-18143966	MS.gene55561:CDS	35.0%
!	TTGATTCGAATGAGGATTCT+CGG	-	chr6.2:18143988-18144007	MS.gene55561:CDS	35.0%
!!	GTTGTTGGTGAATCTTTTCA+TGG	-	chr6.2:18143942-18143961	MS.gene55561:CDS	35.0%
	AAAGTCATCTCACCAAGTGT+CGG	+	chr6.2:18143897-18143916	None:intergenic	40.0%
	AACCTGAAAAGGCTGTTGAA+TGG	+	chr6.2:18144126-18144145	None:intergenic	40.0%
	CGGCAAAATGTTTCACAACA+TGG	-	chr6.2:18144008-18144027	MS.gene55561:CDS	40.0%
	GATATGCAAGGAAAGAAGAG+GGG	-	chr6.2:18143837-18143856	MS.gene55561:CDS	40.0%
	GGTAATTTAGGGAAACCACT+TGG	-	chr6.2:18143645-18143664	MS.gene55561:CDS	40.0%
	TGCATCCAAAACTTGATTCC+CGG	+	chr6.2:18144150-18144169	None:intergenic	40.0%
!	AGAAAACTGTTGATGAGGTG+TGG	-	chr6.2:18143811-18143830	MS.gene55561:CDS	40.0%
!	CTGGTGCTTTGAATTCACAA+GGG	-	chr6.2:18143760-18143779	MS.gene55561:CDS	40.0%
!	GATGACTTTGGAGGATTTCT+TGG	-	chr6.2:18143908-18143927	MS.gene55561:CDS	40.0%
!!	AAAGAAGAGGGGTGTTGATA+GGG	-	chr6.2:18143848-18143867	MS.gene55561:CDS	40.0%
!!	GTTAAGGGTTGATTCGAATG+AGG	-	chr6.2:18143980-18143999	MS.gene55561:CDS	40.0%
	GAGGATTTCTTGGTGAAAGC+TGG	-	chr6.2:18143918-18143937	MS.gene55561:CDS	45.0%
	GAGGTGTGGAAAGATATGCA+AGG	-	chr6.2:18143825-18143844	MS.gene55561:CDS	45.0%
	GTTGTTGCTGCTGAACAGAA+TGG	+	chr6.2:18144058-18144077	None:intergenic	45.0%
	TTGGTGAAAGCTGGAGTTGT+TGG	-	chr6.2:18143927-18143946	MS.gene55561:CDS	45.0%
!	ACTTGGTGAGATGACTTTGG+AGG	-	chr6.2:18143899-18143918	MS.gene55561:CDS	45.0%
!	GACACTTGGTGAGATGACTT+TGG	-	chr6.2:18143896-18143915	MS.gene55561:CDS	45.0%
!	GCTGGTGCTTTGAATTCACA+AGG	-	chr6.2:18143759-18143778	MS.gene55561:CDS	45.0%
!	TGCCATTCAACAGCCTTTTC+AGG	-	chr6.2:18144121-18144140	MS.gene55561:CDS	45.0%
!!	AGTGTATGGAGTGTTGAAGC+TGG	-	chr6.2:18143696-18143715	MS.gene55561:CDS	45.0%
!!	GAAAGAAGAGGGGTGTTGAT+AGG	-	chr6.2:18143847-18143866	MS.gene55561:CDS	45.0%
	CGCGAAAAACAACCGACACT+TGG	-	chr6.2:18143882-18143901	MS.gene55561:CDS	50.0%
	TGATTCCCGGCAACCTGAAA+AGG	+	chr6.2:18144137-18144156	None:intergenic	50.0%
	TGCTGCTGAACAGAATGGAC+CGG	+	chr6.2:18144053-18144072	None:intergenic	50.0%
	TGTTGAAGCTGGCGAGGTTT+CGG	-	chr6.2:18143707-18143726	MS.gene55561:CDS	50.0%
!	GCATATGATGCCGGGTTTTG+CGG	-	chr6.2:18144091-18144110	MS.gene55561:CDS	50.0%
!	GGTTGCCGGGAATCAAGTTT+TGG	-	chr6.2:18144142-18144161	MS.gene55561:CDS	50.0%
!!	AGAGGGGTGTTGATAGGGAT+AGG	-	chr6.2:18143853-18143872	MS.gene55561:CDS	50.0%
!!!	AACAGCCTTTTCAGGTTGCC+GGG	-	chr6.2:18144129-18144148	MS.gene55561:CDS	50.0%
!!!	CAACAGCCTTTTCAGGTTGC+CGG	-	chr6.2:18144128-18144147	MS.gene55561:CDS	50.0%
	AGTGATGTTGCTGCTGCTGC+TGG	-	chr6.2:18143741-18143760	MS.gene55561:CDS	55.0%
	GCATGAACTGCCGCAAAACC+CGG	+	chr6.2:18144104-18144123	None:intergenic	55.0%
	GCTGGCGAGGTTTCGGATTT+CGG	-	chr6.2:18143714-18143733	MS.gene55561:CDS	55.0%
!!	ATGGAGTGTTGAAGCTGGCG+AGG	-	chr6.2:18143701-18143720	MS.gene55561:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	18143543	18144214	18143543	ID=MS.gene55561
chr6.2	mRNA	18143543	18144214	18143543	ID=MS.gene55561.t1;Parent=MS.gene55561
chr6.2	exon	18143543	18144214	18143543	ID=MS.gene55561.t1.exon1;Parent=MS.gene55561.t1
chr6.2	CDS	18143543	18144214	18143543	ID=cds.MS.gene55561.t1;Parent=MS.gene55561.t1

Gene Sequence

>MS.gene55561

ATGGGGTCACAAGGTGGAACAGTTTCAGAGTCAAAGACTTTACCTTTATCTAGGTCAGGATCTTTGTATAATCTAACACTTGATGAAGTTCAAAACCATCTTGGTAATTTAGGGAAACCACTTGGAAGTATGAATCTTGATGAGTTGCTTAAGAGTGTATGGAGTGTTGAAGCTGGCGAGGTTTCGGATTTCGGTGTTAGTGATGTTGCTGCTGCTGCTGGTGCTTTGAATTCACAAGGGAGTTTAACTTTATCTGGAGATCTTAGTAAGAAAACTGTTGATGAGGTGTGGAAAGATATGCAAGGAAAGAAGAGGGGTGTTGATAGGGATAGGAAATCGCGCGAAAAACAACCGACACTTGGTGAGATGACTTTGGAGGATTTCTTGGTGAAAGCTGGAGTTGTTGGTGAATCTTTTCATGGTAAGGAAAATGGTTTGTTAAGGGTTGATTCGAATGAGGATTCTCGGCAAAATGTTTCACAACATGGTCTTCATTGGATGCAATATCCGGTCCATTCTGTTCAGCAGCAACAACATCAGTATGAAAAGCATATGATGCCGGGTTTTGCGGCAGTTCATGCCATTCAACAGCCTTTTCAGGTTGCCGGGAATCAAGTTTTGGATGCAGCGATATCGCCATCTTCTTTAATGGGTACTACTGTTAGAAAATAA

Protein sequence

>MS.gene55561.t1

MGSQGGTVSESKTLPLSRSGSLYNLTLDEVQNHLGNLGKPLGSMNLDELLKSVWSVEAGEVSDFGVSDVAAAAGALNSQGSLTLSGDLSKKTVDEVWKDMQGKKRGVDRDRKSREKQPTLGEMTLEDFLVKAGVVGESFHGKENGLLRVDSNEDSRQNVSQHGLHWMQYPVHSVQQQQHQYEKHMMPGFAAVHAIQQPFQVAGNQVLDAAISPSSLMGTTVRK