AlfalfaGEDB | MS.gene56505

M. sativa cultivar XinJiangDaYe / MS.gene56505

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56505.t1	KEH30808.1	78	246	49	2	1	246	1	241	4.90E-75	291.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56505.t1	A0A072UP61	78.0	246	49	2	1	246	1	241	3.5e-75	291.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049217	MS.gene56505	-0.814754	1.31E-51	-1.69E-46
MS.gene049320	MS.gene56505	-0.802935	4.43E-49	-1.69E-46
MS.gene049611	MS.gene56505	0.810027	1.41E-50	-1.69E-46
MS.gene05104	MS.gene56505	0.81143	7.03E-51	-1.69E-46
MS.gene051366	MS.gene56505	0.802566	5.28E-49	-1.69E-46
MS.gene053420	MS.gene56505	0.827438	1.56E-54	-1.69E-46
MS.gene053421	MS.gene56505	0.804217	2.40E-49	-1.69E-46
MS.gene055154	MS.gene56505	0.807415	5.11E-50	-1.69E-46
MS.gene056657	MS.gene56505	-0.813064	3.09E-51	-1.69E-46
MS.gene059022	MS.gene56505	-0.80464	1.96E-49	-1.69E-46
MS.gene059677	MS.gene56505	-0.809762	1.61E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56505.t1	MTR_4g082365	78.462	260	50	3	1	259	1	255	1.70e-133	377
MS.gene56505.t1	MTR_4g082355	58.392	286	97	4	1	266	1	284	1.16e-91	272
MS.gene56505.t1	MTR_4g082360	59.790	286	89	8	1	266	1	280	1.16e-85	257
MS.gene56505.t1	MTR_4g082550	33.333	306	143	8	7	255	14	315	3.86e-29	113
MS.gene56505.t1	MTR_4g096890	27.458	295	173	13	4	263	12	300	2.42e-12	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56505.t1	AT3G11810	34.295	312	145	7	4	255	14	325	5.51e-39	139
MS.gene56505.t1	AT2G03330	43.548	124	47	4	155	255	239	362	1.67e-11	64.3

Find 62 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCATAAAACTATGGAATTA+AGG	0.231917	4.2:+27338943	None:intergenic
ATTGAAAAGAAGTTGGAAAT+TGG	0.291924	4.2:-27339203	MS.gene56505:CDS
TTTCGATCTGTTACTTGTTT+GGG	0.296706	4.2:-27339156	MS.gene56505:CDS
GAGAGACAAGGAGATAAGTA+TGG	0.352132	4.2:+27339558	None:intergenic
GAGAGAACATCACATATGTT+TGG	0.371892	4.2:+27339724	None:intergenic
TGGGTGGTTGTTGCTGTTGA+AGG	0.382183	4.2:+27339590	None:intergenic
GATAAGTATGGTAGGGTTGA+TGG	0.388328	4.2:+27339570	None:intergenic
AGAAGTGGAGATGGATTTGA+TGG	0.391692	4.2:+27339432	None:intergenic
GGTGGAATCAAGCTGGGGTT+TGG	0.409217	4.2:-27339230	MS.gene56505:CDS
ATCACATATGTTTGGATCCA+TGG	0.412578	4.2:+27339732	None:intergenic
GATGGCTTCTTTGAGTTTGA+CGG	0.428976	4.2:+27339450	None:intergenic
GAGGGGAAGGAGGAAGATGA+GGG	0.434317	4.2:+27339651	None:intergenic
GATGGCTATGAAGGCGCTAT+AGG	0.435117	4.2:+27339507	None:intergenic
GATCAAAAGTTACTCGTACT+TGG	0.436195	4.2:-27339335	MS.gene56505:CDS
TTGTTGTGGTGGAATCAAGC+TGG	0.436772	4.2:-27339237	MS.gene56505:CDS
TTGTTGCTGTTGAAGGTGTT+TGG	0.437194	4.2:+27339597	None:intergenic
AGAGGGGAAGGAGGAAGATG+AGG	0.440647	4.2:+27339650	None:intergenic
ATGGCTTCTTTGAGTTTGAC+GGG	0.448320	4.2:+27339451	None:intergenic
AAAGAAGATGGCAGCGATGA+TGG	0.453937	4.2:+27339384	None:intergenic
TGGAACCATTGAAAAGAAGT+TGG	0.460136	4.2:-27339210	MS.gene56505:CDS
GATGGCTTCGGTGGCAAGGA+GGG	0.471859	4.2:+27339402	None:intergenic
GACAAGGAGATAAGTATGGT+AGG	0.473030	4.2:+27339562	None:intergenic
TGAGAAAAGAGAGGGGAAGG+AGG	0.473897	4.2:+27339641	None:intergenic
AGCATAAGCATTGTTTGTAT+AGG	0.477285	4.2:+27339070	None:intergenic
GGGGAAGAAAGAAGTGGAGA+TGG	0.481542	4.2:+27339423	None:intergenic
GGATGAGAAAAGAGAGGGGA+AGG	0.484211	4.2:+27339638	None:intergenic
ATAAGTATGGTAGGGTTGAT+GGG	0.486358	4.2:+27339571	None:intergenic
TGTTGTGGTGGAATCAAGCT+GGG	0.493170	4.2:-27339236	MS.gene56505:CDS
GATGGCAGCGATGATGGCTT+CGG	0.497874	4.2:+27339390	None:intergenic
ACAAGGAGATAAGTATGGTA+GGG	0.501749	4.2:+27339563	None:intergenic
TGGAGAGGAGGAATTTGAGA+AGG	0.502925	4.2:-27338987	MS.gene56505:CDS
TAGAACAGAAAATTAAGAAG+AGG	0.517168	4.2:+27339536	None:intergenic
CGATGATGGCTTCGGTGGCA+AGG	0.519514	4.2:+27339398	None:intergenic
GTGATATTGCTATGGATAAG+TGG	0.521603	4.2:-27339124	MS.gene56505:CDS
AAGGAGAAGCACACTGAAGT+TGG	0.523808	4.2:-27339007	MS.gene56505:CDS
GGTGAAAGGAATGAGAAAGT+TGG	0.528700	4.2:-27339182	MS.gene56505:CDS
GGAAGATGAGGGAAAGAGTG+AGG	0.538897	4.2:+27339662	None:intergenic
TGGCTTCGGTGGCAAGGAGG+GGG	0.543136	4.2:+27339404	None:intergenic
TCTTAGAAGTGATATTGCTA+TGG	0.547707	4.2:-27339132	MS.gene56505:CDS
GCACACTGAAGTTGGAGAGG+AGG	0.554051	4.2:-27338999	MS.gene56505:CDS
TGATGGCTTCGGTGGCAAGG+AGG	0.554280	4.2:+27339401	None:intergenic
GCTGTAAGTGATGGCTATGA+AGG	0.555536	4.2:+27339498	None:intergenic
TCACATATGTTTGGATCCAT+GGG	0.557128	4.2:+27339733	None:intergenic
GATGAGGATGAGAAAAGAGA+GGG	0.564705	4.2:+27339633	None:intergenic
GGCAGCGATGATGGCTTCGG+TGG	0.568214	4.2:+27339393	None:intergenic
ATTTGAGAAGGCAAAGGACG+AGG	0.569011	4.2:-27338975	MS.gene56505:CDS
AGATGAGGATGAGAAAAGAG+AGG	0.571945	4.2:+27339632	None:intergenic
ATGGCTTCGGTGGCAAGGAG+GGG	0.574035	4.2:+27339403	None:intergenic
AGAAGTTGGAAATTGGTGAA+AGG	0.577351	4.2:-27339196	MS.gene56505:CDS
AGTGAAAGTTATTGTTGTGG+TGG	0.580518	4.2:-27339248	MS.gene56505:CDS
TTTGACGGGTCGATCAAAGA+AGG	0.582848	4.2:+27339465	None:intergenic
ATGAGGATGAGAAAAGAGAG+GGG	0.597244	4.2:+27339634	None:intergenic
GAAGCACACTGAAGTTGGAG+AGG	0.605440	4.2:-27339002	MS.gene56505:CDS
GGAGGAATTTGAGAAGGCAA+AGG	0.622313	4.2:-27338981	MS.gene56505:CDS
CTTAGTGAAAGTTATTGTTG+TGG	0.623664	4.2:-27339251	MS.gene56505:CDS
AATTAAGAAGAGGAGAGACA+AGG	0.629656	4.2:+27339546	None:intergenic
GAAAGAGTGAGGTAGAGACG+AGG	0.632608	4.2:+27339673	None:intergenic
TGGTAATAAGTTGAGAGATG+AGG	0.651317	4.2:+27339617	None:intergenic
GGTGATATACATATACTGCA+AGG	0.674869	4.2:-27339026	MS.gene56505:CDS
GTTGTGGTGGAATCAAGCTG+GGG	0.681004	4.2:-27339235	MS.gene56505:CDS
GTTGTAGATGCTGTAAGTGA+TGG	0.686406	4.2:+27339489	None:intergenic
AGTATGGTAGGGTTGATGGG+TGG	0.699743	4.2:+27339574	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTACAATATTGCTGTTTTTA+CGG	-	chr4.2:27339635-27339654	MS.gene56505:CDS	20.0%
!	AAAAACAAGCATTAAAACCA+TGG	+	chr4.2:27339385-27339404	None:intergenic	25.0%
!	ATTGAAAAGAAGTTGGAAAT+TGG	-	chr4.2:27339479-27339498	MS.gene56505:CDS	25.0%
!	TAAAAAGTTGACAAAGAAGA+TGG	+	chr4.2:27339313-27339332	None:intergenic	25.0%
!	TAGAACAGAAAATTAAGAAG+AGG	+	chr4.2:27339149-27339168	None:intergenic	25.0%
!!	AATTTCCAACTTCTTTTCAA+TGG	+	chr4.2:27339480-27339499	None:intergenic	25.0%
	CTTAGTGAAAGTTATTGTTG+TGG	-	chr4.2:27339431-27339450	MS.gene56505:CDS	30.0%
	TCTTAGAAGTGATATTGCTA+TGG	-	chr4.2:27339550-27339569	MS.gene56505:CDS	30.0%
!	AGCATAAGCATTGTTTGTAT+AGG	+	chr4.2:27339615-27339634	None:intergenic	30.0%
!	TTTCGATCTGTTACTTGTTT+GGG	-	chr4.2:27339526-27339545	MS.gene56505:CDS	30.0%
!!	TTTTCGATCTGTTACTTGTT+TGG	-	chr4.2:27339525-27339544	MS.gene56505:CDS	30.0%
!!!	GCTTGTTTTTATGACGTATT+TGG	-	chr4.2:27339395-27339414	MS.gene56505:CDS	30.0%
	AATTAAGAAGAGGAGAGACA+AGG	+	chr4.2:27339139-27339158	None:intergenic	35.0%
	ACAAGGAGATAAGTATGGTA+GGG	+	chr4.2:27339122-27339141	None:intergenic	35.0%
	AGAAGTTGGAAATTGGTGAA+AGG	-	chr4.2:27339486-27339505	MS.gene56505:CDS	35.0%
	AGTGAAAGTTATTGTTGTGG+TGG	-	chr4.2:27339434-27339453	MS.gene56505:CDS	35.0%
	GAGAGAACATCACATATGTT+TGG	+	chr4.2:27338961-27338980	None:intergenic	35.0%
	GATCAAAAGTTACTCGTACT+TGG	-	chr4.2:27339347-27339366	MS.gene56505:CDS	35.0%
	GGTGATATACATATACTGCA+AGG	-	chr4.2:27339656-27339675	MS.gene56505:CDS	35.0%
	GTGATATTGCTATGGATAAG+TGG	-	chr4.2:27339558-27339577	MS.gene56505:CDS	35.0%
	TGGAACCATTGAAAAGAAGT+TGG	-	chr4.2:27339472-27339491	MS.gene56505:CDS	35.0%
!	ATAAGTATGGTAGGGTTGAT+GGG	+	chr4.2:27339114-27339133	None:intergenic	35.0%
!	TGGTAATAAGTTGAGAGATG+AGG	+	chr4.2:27339068-27339087	None:intergenic	35.0%
!!!	CTTGGTTGTGTTATTTTCCA+TGG	-	chr4.2:27339365-27339384	MS.gene56505:CDS	35.0%
	AGATGAGGATGAGAAAAGAG+AGG	+	chr4.2:27339053-27339072	None:intergenic	40.0%
	ATGAGGATGAGAAAAGAGAG+GGG	+	chr4.2:27339051-27339070	None:intergenic	40.0%
	GACAAGGAGATAAGTATGGT+AGG	+	chr4.2:27339123-27339142	None:intergenic	40.0%
	GAGAGACAAGGAGATAAGTA+TGG	+	chr4.2:27339127-27339146	None:intergenic	40.0%
	GATAAGTATGGTAGGGTTGA+TGG	+	chr4.2:27339115-27339134	None:intergenic	40.0%
	GATGAGGATGAGAAAAGAGA+GGG	+	chr4.2:27339052-27339071	None:intergenic	40.0%
	GGTGAAAGGAATGAGAAAGT+TGG	-	chr4.2:27339500-27339519	MS.gene56505:CDS	40.0%
	GTTGTAGATGCTGTAAGTGA+TGG	+	chr4.2:27339196-27339215	None:intergenic	40.0%
!	AGAAGTGGAGATGGATTTGA+TGG	+	chr4.2:27339253-27339272	None:intergenic	40.0%
!	ATGGCTTCTTTGAGTTTGAC+GGG	+	chr4.2:27339234-27339253	None:intergenic	40.0%
!	GATGGCTTCTTTGAGTTTGA+CGG	+	chr4.2:27339235-27339254	None:intergenic	40.0%
!!	TTGTTGCTGTTGAAGGTGTT+TGG	+	chr4.2:27339088-27339107	None:intergenic	40.0%
	AAAGAAGATGGCAGCGATGA+TGG	+	chr4.2:27339301-27339320	None:intergenic	45.0%
	ATTTGAGAAGGCAAAGGACG+AGG	-	chr4.2:27339707-27339726	MS.gene56505:CDS	45.0%
	GCTGTAAGTGATGGCTATGA+AGG	+	chr4.2:27339187-27339206	None:intergenic	45.0%
	GGAGGAATTTGAGAAGGCAA+AGG	-	chr4.2:27339701-27339720	MS.gene56505:CDS	45.0%
	TGGAGAGGAGGAATTTGAGA+AGG	-	chr4.2:27339695-27339714	MS.gene56505:CDS	45.0%
!	AAGGAGAAGCACACTGAAGT+TGG	-	chr4.2:27339675-27339694	MS.gene56505:CDS	45.0%
!	TGTTGTGGTGGAATCAAGCT+GGG	-	chr4.2:27339446-27339465	MS.gene56505:CDS	45.0%
!	TTGTTGTGGTGGAATCAAGC+TGG	-	chr4.2:27339445-27339464	MS.gene56505:CDS	45.0%
!	TTTGACGGGTCGATCAAAGA+AGG	+	chr4.2:27339220-27339239	None:intergenic	45.0%
	AAGGAGGGGGAAGAAAGAAG+TGG	+	chr4.2:27339268-27339287	None:intergenic	50.0%
	GAAAGAGTGAGGTAGAGACG+AGG	+	chr4.2:27339012-27339031	None:intergenic	50.0%
	GAAGCACACTGAAGTTGGAG+AGG	-	chr4.2:27339680-27339699	MS.gene56505:CDS	50.0%
	GGAAGATGAGGGAAAGAGTG+AGG	+	chr4.2:27339023-27339042	None:intergenic	50.0%
	GGATGAGAAAAGAGAGGGGA+AGG	+	chr4.2:27339047-27339066	None:intergenic	50.0%
	GGGGAAGAAAGAAGTGGAGA+TGG	+	chr4.2:27339262-27339281	None:intergenic	50.0%
	TGAGAAAAGAGAGGGGAAGG+AGG	+	chr4.2:27339044-27339063	None:intergenic	50.0%
!	GTTGTGGTGGAATCAAGCTG+GGG	-	chr4.2:27339447-27339466	MS.gene56505:CDS	50.0%
!	TGGGTGGTTGTTGCTGTTGA+AGG	+	chr4.2:27339095-27339114	None:intergenic	50.0%
!!	AGTATGGTAGGGTTGATGGG+TGG	+	chr4.2:27339111-27339130	None:intergenic	50.0%
!!	GATGGCTATGAAGGCGCTAT+AGG	+	chr4.2:27339178-27339197	None:intergenic	50.0%
	AGAGGGGAAGGAGGAAGATG+AGG	+	chr4.2:27339035-27339054	None:intergenic	55.0%
	GAGGGGAAGGAGGAAGATGA+GGG	+	chr4.2:27339034-27339053	None:intergenic	55.0%
	GCACACTGAAGTTGGAGAGG+AGG	-	chr4.2:27339683-27339702	MS.gene56505:CDS	55.0%
	GGTGGAATCAAGCTGGGGTT+TGG	-	chr4.2:27339452-27339471	MS.gene56505:CDS	55.0%
!	GATGGCAGCGATGATGGCTT+CGG	+	chr4.2:27339295-27339314	None:intergenic	55.0%
	ATGGCTTCGGTGGCAAGGAG+GGG	+	chr4.2:27339282-27339301	None:intergenic	60.0%
	GATGGCTTCGGTGGCAAGGA+GGG	+	chr4.2:27339283-27339302	None:intergenic	60.0%
	TGATGGCTTCGGTGGCAAGG+AGG	+	chr4.2:27339284-27339303	None:intergenic	60.0%
!	CGATGATGGCTTCGGTGGCA+AGG	+	chr4.2:27339287-27339306	None:intergenic	60.0%
	TGGCTTCGGTGGCAAGGAGG+GGG	+	chr4.2:27339281-27339300	None:intergenic	65.0%
!	GGCAGCGATGATGGCTTCGG+TGG	+	chr4.2:27339292-27339311	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	27338952	27339752	27338952	ID=MS.gene56505
chr4.2	mRNA	27338952	27339752	27338952	ID=MS.gene56505.t1;Parent=MS.gene56505
chr4.2	exon	27338952	27339752	27338952	ID=MS.gene56505.t1.exon1;Parent=MS.gene56505.t1
chr4.2	CDS	27338952	27339752	27338952	ID=cds.MS.gene56505.t1;Parent=MS.gene56505.t1

Gene Sequence

>MS.gene56505

ATGGATCCAAACATATGTGATGTTCTCTCTGAATCTAAACGCATAATAAACTCTCAACCTCGTCTCTACCTCACTCTTTCCCTCATCTTCCTCCTTCCCCTCTCTTTTCTCATCCTCATCTCTCAACTTATTACCAAACACCTTCAACAGCAACAACCACCCATCAACCCTACCATACTTATCTCCTTGTCTCTCCTCTTCTTAATTTTCTGTTCTATCTTCTCCTATAGCGCCTTCATAGCCATCACTTACAGCATCTACAACGCCTTCTTTGATCGACCCGTCAAACTCAAAGAAGCCATCAAATCCATCTCCACTTCTTTCTTCCCCCTCCTTGCCACCGAAGCCATCATCGCTGCCATCTTCTTTGTCAACTTTTTACTATTTGTATATCTGATCAAAAGTTACTCGTACTTGGTTGTGTTATTTTCCATGGTTTTAATGCTTGTTTTTATGACGTATTTGGTAGTTAACTTGAGCTTAGTGAAAGTTATTGTTGTGGTGGAATCAAGCTGGGGTTTGGAACCATTGAAAAGAAGTTGGAAATTGGTGAAAGGAATGAGAAAGTTGGTATTTTCGATCTGTTACTTGTTTGGGTTCTTAGAAGTGATATTGCTATGGATAAGTGGTTACAATTTAGTACTGATTTTCGTGATTTCTCCTATACAAACAATGCTTATGCTTTACAATATTGCTGTTTTTACGGTGATATACATATACTGCAAGGAGAAGCACACTGAAGTTGGAGAGGAGGAATTTGAGAAGGCAAAGGACGAGGCTAGTTTGTCCTTAATTCCATAG

Protein sequence

>MS.gene56505.t1

MDPNICDVLSESKRIINSQPRLYLTLSLIFLLPLSFLILISQLITKHLQQQQPPINPTILISLSLLFLIFCSIFSYSAFIAITYSIYNAFFDRPVKLKEAIKSISTSFFPLLATEAIIAAIFFVNFLLFVYLIKSYSYLVVLFSMVLMLVFMTYLVVNLSLVKVIVVVESSWGLEPLKRSWKLVKGMRKLVFSICYLFGFLEVILLWISGYNLVLIFVISPIQTMLMLYNIAVFTVIYIYCKEKHTEVGEEEFEKAKDEASLSLIP