AlfalfaGEDB | MS.gene56646

M. sativa cultivar XinJiangDaYe / MS.gene56646

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56646.t1	XP_003596225.2	93.5	155	10	0	1	155	1	155	5.00E-80	307

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56646.t1	G7IJC5	93.5	155	10	0	1	155	1	155	3.6e-80	307.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049404	MS.gene56646	0.821586	3.73E-53	-1.69E-46
MS.gene050273	MS.gene56646	0.812574	3.96E-51	-1.69E-46
MS.gene050559	MS.gene56646	0.80042	1.45E-48	-1.69E-46
MS.gene050807	MS.gene56646	0.801754	7.75E-49	-1.69E-46
MS.gene051279	MS.gene56646	0.835581	1.54E-56	-1.69E-46
MS.gene052239	MS.gene56646	-0.847846	8.93E-60	-1.69E-46
MS.gene052447	MS.gene56646	0.80008	1.70E-48	-1.69E-46
MS.gene055464	MS.gene56646	0.819585	1.08E-52	-1.69E-46
MS.gene057287	MS.gene56646	0.802125	6.51E-49	-1.69E-46
MS.gene059015	MS.gene56646	0.812531	4.05E-51	-1.69E-46
MS.gene059584	MS.gene56646	0.801925	7.15E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56646.t1	MTR_2g071790	93.548	155	10	0	1	155	1	155	3.24e-105	297
MS.gene56646.t1	MTR_2g071760	87.742	155	19	0	1	155	1	155	3.14e-96	275
MS.gene56646.t1	MTR_2g071750	85.806	155	22	0	1	155	1	155	8.82e-96	273
MS.gene56646.t1	MTR_2g071740	85.621	153	16	2	1	152	1	148	1.16e-89	258

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 40 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGGGTTGAGTGGGTGGATA+TGG	0.201977	2.1:+27327715	MS.gene56646:CDS
TGTGATGGTTGATCATTTAA+TGG	0.277636	2.1:+27327808	MS.gene56646:CDS
CCCAACTTGCATCATCAAAT+TGG	0.284176	2.1:-27327754	None:intergenic
TTGTGATGATGGGTTTGATA+TGG	0.302331	2.1:+27327832	MS.gene56646:CDS
TGGAGGAGGGACATGACTTT+GGG	0.323166	2.1:+27327696	MS.gene56646:CDS
AACATGCTTACAATTCCTTT+TGG	0.331685	2.1:+27327858	MS.gene56646:CDS
CATTTAATGGTTTGTGATGA+TGG	0.359988	2.1:+27327821	MS.gene56646:CDS
CCAATTTGATGATGCAAGTT+GGG	0.373282	2.1:+27327754	MS.gene56646:CDS
AAGAATATTATGATGAATCA+TGG	0.395210	2.1:+27327657	MS.gene56646:CDS
ATGGAGGAGGGACATGACTT+TGG	0.417476	2.1:+27327695	MS.gene56646:CDS
TGCTTGTGATGTGGAAATCA+AGG	0.457434	2.1:+27327472	MS.gene56646:CDS
GACATGACTTTGGGGTTGAG+TGG	0.467986	2.1:+27327705	MS.gene56646:CDS
TCCAATTTGATGATGCAAGT+TGG	0.473251	2.1:+27327753	MS.gene56646:CDS
ATTTAATGGTTTGTGATGAT+GGG	0.498358	2.1:+27327822	MS.gene56646:CDS
ACATGACTTTGGGGTTGAGT+GGG	0.501007	2.1:+27327706	MS.gene56646:CDS
TGAAATTAGTGAAAGCAAGA+TGG	0.502049	2.1:+27327583	MS.gene56646:CDS
TGATCTTCTCACGTCACTGA+TGG	0.502867	2.1:+27327496	MS.gene56646:CDS
CTCATAGCTATCTTGCCAAA+AGG	0.504425	2.1:-27327873	None:intergenic
CTATGCATGTTGCATTATCA+TGG	0.506444	2.1:-27327610	None:intergenic
TTGGCAAGATAGCTATGAGA+TGG	0.516169	2.1:+27327877	MS.gene56646:CDS
TGGCAAGATAGCTATGAGAT+GGG	0.520842	2.1:+27327878	MS.gene56646:CDS
TGACTTTGGGGTTGAGTGGG+TGG	0.527133	2.1:+27327709	MS.gene56646:CDS
TATGATGAATCATGGAACAT+AGG	0.530061	2.1:+27327665	MS.gene56646:CDS
CATGATAATGCAACATGCAT+AGG	0.538210	2.1:+27327611	MS.gene56646:CDS
GAAATTAGTGAAAGCAAGAT+GGG	0.562708	2.1:+27327584	MS.gene56646:CDS
ATGGAACATAGGACAAGTGA+TGG	0.569406	2.1:+27327676	MS.gene56646:CDS
GGAGGAGGGACATGACTTTG+GGG	0.584089	2.1:+27327697	MS.gene56646:CDS
TGGGAGGCAATTGAGTCTTG+TGG	0.585282	2.1:+27327773	MS.gene56646:CDS
CATAGGACAAGTGATGGAGG+AGG	0.595117	2.1:+27327682	MS.gene56646:CDS
ACTTGCATCATCAAATTGGA+AGG	0.605601	2.1:-27327750	None:intergenic
TGGAGATGAGAAAGATGTGA+TGG	0.610494	2.1:+27327793	MS.gene56646:CDS
GCTTGTGATGTGGAAATCAA+GGG	0.616324	2.1:+27327473	MS.gene56646:CDS
GGCAAGATAGCTATGAGATG+GGG	0.624212	2.1:+27327879	MS.gene56646:CDS
AGATAGCTATGAGATGGGGT+TGG	0.626818	2.1:+27327883	MS.gene56646:CDS
ATTTGATGATGCAAGTTGGG+AGG	0.639497	2.1:+27327757	MS.gene56646:CDS
GAACATAGGACAAGTGATGG+AGG	0.641704	2.1:+27327679	MS.gene56646:CDS
TCATCATTACTCTCATCACA+TGG	0.648258	2.1:-27327527	None:intergenic
GTGATGAGAGTAATGATGAA+AGG	0.650915	2.1:+27327531	MS.gene56646:CDS
ATAGGACAAGTGATGGAGGA+GGG	0.671083	2.1:+27327683	MS.gene56646:CDS
ATTAGTGAAAGCAAGATGGG+TGG	0.695184	2.1:+27327587	MS.gene56646:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGAATATTATGATGAATCA+TGG	+	chr2.1:27327657-27327676	MS.gene56646:CDS	20.0%
!!!	TAGTTTAATAAGATCTTTTG+AGG	+	chr2.1:27327559-27327578	MS.gene56646:CDS	20.0%
!!	ATTTAATGGTTTGTGATGAT+GGG	+	chr2.1:27327822-27327841	MS.gene56646:CDS	25.0%
	GAAATTAGTGAAAGCAAGAT+GGG	+	chr2.1:27327584-27327603	MS.gene56646:CDS	30.0%
	TATGATGAATCATGGAACAT+AGG	+	chr2.1:27327665-27327684	MS.gene56646:CDS	30.0%
	TGAAATTAGTGAAAGCAAGA+TGG	+	chr2.1:27327583-27327602	MS.gene56646:CDS	30.0%
	TGTGATGGTTGATCATTTAA+TGG	+	chr2.1:27327808-27327827	MS.gene56646:CDS	30.0%
!	AACATGCTTACAATTCCTTT+TGG	+	chr2.1:27327858-27327877	MS.gene56646:CDS	30.0%
!	CATTTAATGGTTTGTGATGA+TGG	+	chr2.1:27327821-27327840	MS.gene56646:CDS	30.0%
	ACTTGCATCATCAAATTGGA+AGG	-	chr2.1:27327753-27327772	None:intergenic	35.0%
	CTATGCATGTTGCATTATCA+TGG	-	chr2.1:27327613-27327632	None:intergenic	35.0%
	GTGATGAGAGTAATGATGAA+AGG	+	chr2.1:27327531-27327550	MS.gene56646:CDS	35.0%
	TCATCATTACTCTCATCACA+TGG	-	chr2.1:27327530-27327549	None:intergenic	35.0%
!	CATGATAATGCAACATGCAT+AGG	+	chr2.1:27327611-27327630	MS.gene56646:CDS	35.0%
!	TGATACTTTTGCTTGTGATG+TGG	+	chr2.1:27327463-27327482	MS.gene56646:CDS	35.0%
!!	CCAATTTGATGATGCAAGTT+GGG	+	chr2.1:27327754-27327773	MS.gene56646:CDS	35.0%
!!	TCCAATTTGATGATGCAAGT+TGG	+	chr2.1:27327753-27327772	MS.gene56646:CDS	35.0%
!!	TTGTGATGATGGGTTTGATA+TGG	+	chr2.1:27327832-27327851	MS.gene56646:CDS	35.0%
	ATGGAACATAGGACAAGTGA+TGG	+	chr2.1:27327676-27327695	MS.gene56646:CDS	40.0%
	ATTAGTGAAAGCAAGATGGG+TGG	+	chr2.1:27327587-27327606	MS.gene56646:CDS	40.0%
	CCCAACTTGCATCATCAAAT+TGG	-	chr2.1:27327757-27327776	None:intergenic	40.0%
	CTCATAGCTATCTTGCCAAA+AGG	-	chr2.1:27327876-27327895	None:intergenic	40.0%
	GCTTGTGATGTGGAAATCAA+GGG	+	chr2.1:27327473-27327492	MS.gene56646:CDS	40.0%
	TGCTTGTGATGTGGAAATCA+AGG	+	chr2.1:27327472-27327491	MS.gene56646:CDS	40.0%
	TGGAGATGAGAAAGATGTGA+TGG	+	chr2.1:27327793-27327812	MS.gene56646:CDS	40.0%
	TGGCAAGATAGCTATGAGAT+GGG	+	chr2.1:27327878-27327897	MS.gene56646:CDS	40.0%
	TTGGCAAGATAGCTATGAGA+TGG	+	chr2.1:27327877-27327896	MS.gene56646:CDS	40.0%
!!	ATTTGATGATGCAAGTTGGG+AGG	+	chr2.1:27327757-27327776	MS.gene56646:CDS	40.0%
	AGATAGCTATGAGATGGGGT+TGG	+	chr2.1:27327883-27327902	MS.gene56646:CDS	45.0%
	GAACATAGGACAAGTGATGG+AGG	+	chr2.1:27327679-27327698	MS.gene56646:CDS	45.0%
	GGCAAGATAGCTATGAGATG+GGG	+	chr2.1:27327879-27327898	MS.gene56646:CDS	45.0%
	TGATCTTCTCACGTCACTGA+TGG	+	chr2.1:27327496-27327515	MS.gene56646:CDS	45.0%
!	ATAGGACAAGTGATGGAGGA+GGG	+	chr2.1:27327683-27327702	MS.gene56646:CDS	45.0%
!!	ACATGACTTTGGGGTTGAGT+GGG	+	chr2.1:27327706-27327725	MS.gene56646:CDS	45.0%
	ATGGAGGAGGGACATGACTT+TGG	+	chr2.1:27327695-27327714	MS.gene56646:CDS	50.0%
	TGGAGGAGGGACATGACTTT+GGG	+	chr2.1:27327696-27327715	MS.gene56646:CDS	50.0%
	TGGGAGGCAATTGAGTCTTG+TGG	+	chr2.1:27327773-27327792	MS.gene56646:CDS	50.0%
!	CATAGGACAAGTGATGGAGG+AGG	+	chr2.1:27327682-27327701	MS.gene56646:CDS	50.0%
!!	GACATGACTTTGGGGTTGAG+TGG	+	chr2.1:27327705-27327724	MS.gene56646:CDS	50.0%
	GGAGGAGGGACATGACTTTG+GGG	+	chr2.1:27327697-27327716	MS.gene56646:CDS	55.0%
	TGGGGTTGAGTGGGTGGATA+TGG	+	chr2.1:27327715-27327734	MS.gene56646:CDS	55.0%
!!	TGACTTTGGGGTTGAGTGGG+TGG	+	chr2.1:27327709-27327728	MS.gene56646:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	27327446	27327913	27327446	ID=MS.gene56646
chr2.1	mRNA	27327446	27327913	27327446	ID=MS.gene56646.t1;Parent=MS.gene56646
chr2.1	exon	27327446	27327913	27327446	ID=MS.gene56646.t1.exon1;Parent=MS.gene56646.t1
chr2.1	CDS	27327446	27327913	27327446	ID=cds.MS.gene56646.t1;Parent=MS.gene56646.t1

Gene Sequence

>MS.gene56646

ATGGCTTCTCAAGTTTGTGATACTTTTGCTTGTGATGTGGAAATCAAGGGTGATCTTCTCACGTCACTGATGGAAGAGTTACCATGTGATGAGAGTAATGATGAAAGGTTAGATAGTTTAATAAGATCTTTTGAGGCTGAAATTAGTGAAAGCAAGATGGGTGGCCATGATAATGCAACATGCATAGGATCACAATTGAAGAGCAATTTTGAAGAATATTATGATGAATCATGGAACATAGGACAAGTGATGGAGGAGGGACATGACTTTGGGGTTGAGTGGGTGGATATGGATTTGATGACATCCTTCCAATTTGATGATGCAAGTTGGGAGGCAATTGAGTCTTGTGGAGATGAGAAAGATGTGATGGTTGATCATTTAATGGTTTGTGATGATGGGTTTGATATGGAAGAACATGCTTACAATTCCTTTTGGCAAGATAGCTATGAGATGGGGTTGGTGCATTAG

Protein sequence

>MS.gene56646.t1

MASQVCDTFACDVEIKGDLLTSLMEELPCDESNDERLDSLIRSFEAEISESKMGGHDNATCIGSQLKSNFEEYYDESWNIGQVMEEGHDFGVEWVDMDLMTSFQFDDASWEAIESCGDEKDVMVDHLMVCDDGFDMEEHAYNSFWQDSYEMGLVH