AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56980


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56980.t1 MTR_5g018365 96.951 164 5 0 1 164 6 169 4.66e-117 332
MS.gene56980.t1 MTR_5g018350 74.390 164 42 0 1 164 1 164 9.30e-86 252
MS.gene56980.t1 MTR_8g079120 61.875 160 61 0 5 164 51 210 2.40e-71 214
MS.gene56980.t1 MTR_8g079120 61.875 160 61 0 5 164 9 168 1.85e-70 213
MS.gene56980.t1 MTR_8g007675 46.296 162 81 3 7 164 32 191 1.70e-44 148
MS.gene56980.t1 MTR_5g018320 43.558 163 85 2 7 164 5 165 9.69e-41 137
MS.gene56980.t1 MTR_5g018300 41.615 161 87 2 5 160 6 164 2.00e-39 134
MS.gene56980.t1 MTR_8g008505 42.857 161 89 2 6 164 27 186 9.08e-39 133
MS.gene56980.t1 MTR_3g464040 41.975 162 87 2 6 164 18 175 1.60e-38 132
MS.gene56980.t1 MTR_8g008500 43.293 164 88 3 6 164 26 189 3.46e-38 132
MS.gene56980.t1 MTR_8g007670 43.373 166 84 5 1 160 1 162 1.07e-37 130
MS.gene56980.t1 MTR_8g007670 52.294 109 52 0 1 109 1 109 3.79e-37 127
MS.gene56980.t1 MTR_8g008660 52.885 104 49 0 6 109 26 129 1.09e-36 127
MS.gene56980.t1 MTR_5g018280 50.943 106 52 0 5 110 6 111 2.06e-35 124
MS.gene56980.t1 MTR_3g093730 47.115 104 55 0 4 107 33 136 3.44e-32 115
MS.gene56980.t1 MTR_3g093730 47.115 104 55 0 4 107 33 136 1.49e-31 114
MS.gene56980.t1 MTR_5g068100 47.573 103 54 0 5 107 28 130 2.13e-30 111
MS.gene56980.t1 MTR_7g015220 47.619 105 55 0 6 110 123 227 5.47e-28 107
MS.gene56980.t1 MTR_3g005410 46.552 116 62 0 1 116 88 203 9.62e-28 105
MS.gene56980.t1 MTR_1g034410 53.165 79 17 3 28 93 39 110 5.39e-14 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56980.t1 AT2G23600 47.904 167 83 3 2 164 19 185 3.81e-55 172
MS.gene56980.t1 AT2G23620 53.750 160 69 3 3 160 5 161 3.88e-55 174
MS.gene56980.t1 AT2G23600 48.193 166 82 3 3 164 20 185 6.17e-54 172
MS.gene56980.t1 AT2G23600 48.193 166 82 3 3 164 5 170 6.45e-54 171
MS.gene56980.t1 AT5G10300 52.410 166 73 2 3 164 2 165 7.80e-52 164
MS.gene56980.t1 AT5G10300 52.410 166 73 2 3 164 2 165 4.49e-51 164
MS.gene56980.t1 AT2G23590 46.250 160 81 1 5 164 25 179 2.76e-50 162
MS.gene56980.t1 AT2G23580 47.904 167 82 3 3 164 4 170 3.86e-49 157
MS.gene56980.t1 AT2G23610 44.910 167 88 2 2 164 4 170 6.62e-49 159
MS.gene56980.t1 AT2G23580 47.904 167 82 3 3 164 4 170 9.27e-49 158
MS.gene56980.t1 AT2G23550 49.080 163 78 3 2 159 10 172 2.05e-48 155
MS.gene56980.t1 AT2G23550 49.080 163 78 3 2 159 10 172 1.31e-47 155
MS.gene56980.t1 AT2G23550 49.080 163 78 3 2 159 3 165 1.50e-47 155
MS.gene56980.t1 AT2G23610 53.600 125 57 1 2 125 4 128 6.15e-47 152
MS.gene56980.t1 AT2G23560 42.683 164 92 2 3 164 4 167 1.84e-44 147
MS.gene56980.t1 AT3G50440 42.857 161 88 1 8 164 22 182 8.49e-43 143
MS.gene56980.t1 AT4G37150 52.941 119 56 0 6 124 2 120 1.74e-42 142
MS.gene56980.t1 AT4G37140 53.636 110 51 0 5 114 1 110 1.89e-40 133
MS.gene56980.t1 AT4G37140 56.044 91 40 0 6 96 2 92 1.79e-33 114
MS.gene56980.t1 AT3G10870 45.283 106 58 0 2 107 15 120 1.98e-31 114
MS.gene56980.t1 AT2G23550 40.123 162 65 3 2 159 3 136 1.02e-30 111
MS.gene56980.t1 AT3G29770 53.922 102 47 0 6 107 137 238 1.72e-29 111
MS.gene56980.t1 AT1G33990 47.748 111 58 0 6 116 96 206 1.99e-28 107
MS.gene56980.t1 AT4G09900 46.903 113 60 0 4 116 95 207 2.97e-28 107
MS.gene56980.t1 AT1G69240 47.059 102 54 0 6 107 186 287 2.07e-26 103
MS.gene56980.t1 AT1G26360 47.059 102 54 0 6 107 188 289 8.00e-26 102
MS.gene56980.t1 AT5G58310 34.884 172 96 4 5 164 3 170 1.10e-25 99.0
MS.gene56980.t1 AT4G16690 47.525 101 53 0 7 107 12 112 6.39e-25 97.1
MS.gene56980.t1 AT3G29770 50.000 74 37 0 6 79 137 210 4.86e-16 73.6
MS.gene56980.t1 AT2G23570 43.023 86 45 1 83 164 1 86 1.76e-14 67.8

Find 25 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGGTTTGTGTTGAGTATC+AGG 0.310434 5.1:-10455094 None:intergenic
ATTGAGAGATACCCGGTTAC+TGG 0.326715 5.1:+10456019 MS.gene56980:CDS
GTTCTTGTTGGACATAGCTT+TGG 0.363914 5.1:+10454995 MS.gene56980:CDS
GTGTCTAACCATCCAGTAAC+CGG 0.382878 5.1:-10456031 None:intergenic
GAGTATGCTAAGCCTTTGTT+AGG 0.417352 5.1:+10454938 MS.gene56980:CDS
CTTGTTGGACATAGCTTTGG+AGG 0.435184 5.1:+10454998 MS.gene56980:CDS
ACCATCATATGTTCTAGAAC+AGG 0.465534 5.1:+10455111 MS.gene56980:CDS
AACAAAATGCTGTTGTTACC+TGG 0.480280 5.1:+10456073 MS.gene56980:CDS
CTAGTACATGGTGTTAGCGT+TGG 0.485280 5.1:+10454794 MS.gene56980:CDS
ATGGTGTTAGCGTTGGAGCT+TGG 0.496712 5.1:+10454801 MS.gene56980:CDS
ACCTGTTCTAGAACATATGA+TGG 0.511075 5.1:-10455112 None:intergenic
CTTTCAACTCTGCTCCATTG+AGG 0.515282 5.1:+10456120 MS.gene56980:CDS
TTAGCGTTGGAGCTTGGAGT+TGG 0.518739 5.1:+10454807 MS.gene56980:CDS
GGCACAATTAAGTACCTCAA+TGG 0.530565 5.1:-10456134 None:intergenic
GTAGACAAGAACTTTGAGCC+AGG 0.559075 5.1:-10456091 None:intergenic
TATCAGGAATAAAAGCTGCT+AGG 0.583140 5.1:-10455078 None:intergenic
AGAGATACCCGGTTACTGGA+TGG 0.592966 5.1:+10456023 MS.gene56980:CDS
AATGAGTATAGCTCTTGCAA+TGG 0.600234 5.1:+10455021 MS.gene56980:CDS
ACACTTGACCTTGCAGCTAG+TGG 0.615932 5.1:+10454875 MS.gene56980:CDS
TGACCTACAGATTCTAACTG+TGG 0.617711 5.1:-10454842 None:intergenic
TGTCTAACCATCCAGTAACC+GGG 0.625170 5.1:-10456030 None:intergenic
TGTTGATGCCACTAGCTGCA+AGG 0.665739 5.1:-10454883 None:intergenic
AAACCACAGTTAGAATCTGT+AGG 0.674566 5.1:+10454839 MS.gene56980:CDS
GAGGCTAAGAAGCCTAACAA+AGG 0.687792 5.1:-10454950 None:intergenic
ATAGTATATTGAGAGATACC+CGG 0.707415 5.1:+10456012 MS.gene56980:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATAAATCTAATTTATCA+AGG - chr5.1:10455931-10455950 None:intergenic 10.0%
!!! AATATTTATTAGTTTAGTTA+TGG + chr5.1:10455494-10455513 MS.gene56980:intron 10.0%
!!! ATATTTATTAGTTTAGTTAT+GGG + chr5.1:10455495-10455514 MS.gene56980:intron 10.0%
!!! AATATTGTTAATTTTCTTAG+TGG + chr5.1:10455219-10455238 MS.gene56980:intron 15.0%
!! ATATAGTGATTATACCTAAA+GGG - chr5.1:10455806-10455825 None:intergenic 20.0%
!! TATATAGTGATTATACCTAA+AGG - chr5.1:10455807-10455826 None:intergenic 20.0%
!!! ACACCTTCTAAAAAAATAAA+CGG - chr5.1:10455683-10455702 None:intergenic 20.0%
!!! TGATGATATTTGTTTTAAGT+TGG + chr5.1:10455469-10455488 MS.gene56980:intron 20.0%
! AAGTAGTTAAATACACCTTA+AGG - chr5.1:10455575-10455594 None:intergenic 25.0%
! AAGTTGTTAAATACACCTTA+AGG - chr5.1:10455722-10455741 None:intergenic 25.0%
! ACACCTTAATAAAAACTAGT+GGG + chr5.1:10455765-10455784 MS.gene56980:intron 25.0%
! TATAGTGATTATACCTAAAG+GGG - chr5.1:10455805-10455824 None:intergenic 25.0%
! TTATCCTAGTTTACATTTGA+TGG - chr5.1:10455292-10455311 None:intergenic 25.0%
! TTTACCATCAAATGTAAACT+AGG + chr5.1:10455285-10455304 MS.gene56980:intron 25.0%
! TTTCCTGAAAAAATTGAAGT+TGG + chr5.1:10455049-10455068 MS.gene56980:CDS 25.0%
!! ACACCTTCTAAAAAATAAGT+GGG + chr5.1:10455617-10455636 MS.gene56980:intron 25.0%
!! CACCTTCTAAAAAAATAAAC+GGG - chr5.1:10455682-10455701 None:intergenic 25.0%
!! TTTTAGCAATATTCACCTTA+AGG + chr5.1:10455704-10455723 MS.gene56980:intron 25.0%
!!! ATTCCAACTTCAATTTTTTC+AGG - chr5.1:10455055-10455074 None:intergenic 25.0%
AAAAAAATACGTCAACAGCA+GGG + chr5.1:10455378-10455397 MS.gene56980:intron 30.0%
AATGAAAAAGTGGTTCTTGT+TGG + chr5.1:10454983-10455002 MS.gene56980:CDS 30.0%
ACTTGATCCAAATGAAAAAG+TGG + chr5.1:10454973-10454992 MS.gene56980:CDS 30.0%
ATAGTATATTGAGAGATACC+CGG + chr5.1:10456012-10456031 MS.gene56980:intron 30.0%
CACACCTTAATAAAAACTAG+TGG + chr5.1:10455764-10455783 MS.gene56980:intron 30.0%
TAAAAAAATACGTCAACAGC+AGG + chr5.1:10455377-10455396 MS.gene56980:intron 30.0%
! AAGCATTTTGTTCTAGTACA+TGG + chr5.1:10454782-10454801 MS.gene56980:CDS 30.0%
! ACTTGGATCATTTTGTGATA+TGG + chr5.1:10455989-10456008 MS.gene56980:intron 30.0%
! CACACCTTCTAAAAAATAAG+TGG + chr5.1:10455616-10455635 MS.gene56980:intron 30.0%
! GAACAAAATGCTTCTGTTTT+TGG - chr5.1:10454775-10454794 None:intergenic 30.0%
! TTAGACACTGAGTTTTCATT+TGG + chr5.1:10456046-10456065 MS.gene56980:CDS 30.0%
! TTTTTCATTTGGATCAAGTG+AGG - chr5.1:10454972-10454991 None:intergenic 30.0%
!!! ACAAGAACCACTTTTTCATT+TGG - chr5.1:10454983-10455002 None:intergenic 30.0%
!!! ACACCCACTAGTTTTTATTA+AGG - chr5.1:10455771-10455790 None:intergenic 30.0%
!!! ACACCCACTTATTTTTTAGA+AGG - chr5.1:10455623-10455642 None:intergenic 30.0%
!!! CACCCGTTTATTTTTTTAGA+AGG + chr5.1:10455677-10455696 MS.gene56980:intron 30.0%
AAACCACAGTTAGAATCTGT+AGG + chr5.1:10454839-10454858 MS.gene56980:CDS 35.0%
AACAAAATGCTGTTGTTACC+TGG + chr5.1:10456073-10456092 MS.gene56980:CDS 35.0%
AATGAGTATAGCTCTTGCAA+TGG + chr5.1:10455021-10455040 MS.gene56980:CDS 35.0%
ACTCTCACATAAAAACCAGT+GGG - chr5.1:10455536-10455555 None:intergenic 35.0%
AGACAGACTTGAAATCTGTT+GGG - chr5.1:10455425-10455444 None:intergenic 35.0%
ATCACAAAATGATCCAAGTC+AGG - chr5.1:10455988-10456007 None:intergenic 35.0%
TATCAGGAATAAAAGCTGCT+AGG - chr5.1:10455081-10455100 None:intergenic 35.0%
TGTATGTTAGCAACTCCTAT+AGG - chr5.1:10455659-10455678 None:intergenic 35.0%
TTTATTTACCTGACCTGACT+TGG + chr5.1:10455972-10455991 MS.gene56980:intron 35.0%
! TTTTAGCAAGCTACACCTTA+AGG + chr5.1:10455557-10455576 MS.gene56980:intron 35.0%
!! ACCATCATATGTTCTAGAAC+AGG + chr5.1:10455111-10455130 MS.gene56980:CDS 35.0%
!! ACCTGTTCTAGAACATATGA+TGG - chr5.1:10455115-10455134 None:intergenic 35.0%
AAAATGATCCAAGTCAGGTC+AGG - chr5.1:10455983-10456002 None:intergenic 40.0%
AACTCCTATAGGCATTTGCT+AGG - chr5.1:10455648-10455667 None:intergenic 40.0%
CACTCTCACATAAAAACCAG+TGG - chr5.1:10455537-10455556 None:intergenic 40.0%
GAGACAGACTTGAAATCTGT+TGG - chr5.1:10455426-10455445 None:intergenic 40.0%
GAGTATGCTAAGCCTTTGTT+AGG + chr5.1:10454938-10454957 MS.gene56980:CDS 40.0%
GTATCCTAGCAAATGCCTAT+AGG + chr5.1:10455641-10455660 MS.gene56980:intron 40.0%
GTTCTTGTTGGACATAGCTT+TGG + chr5.1:10454995-10455014 MS.gene56980:CDS 40.0%
TCAATATGTCCGAGTGGTTA+AGG - chr5.1:10455448-10455467 None:intergenic 40.0%
TGACCTACAGATTCTAACTG+TGG - chr5.1:10454845-10454864 None:intergenic 40.0%
! GACACTGAGTTTTCATTTGG+TGG + chr5.1:10456049-10456068 MS.gene56980:CDS 40.0%
! GATGGTTTGTGTTGAGTATC+AGG - chr5.1:10455097-10455116 None:intergenic 40.0%
! TATACCTAAAGGGGTTTGCT+AGG - chr5.1:10455796-10455815 None:intergenic 40.0%
AGTCTGTCTCCTTAACCACT+CGG + chr5.1:10455436-10455455 MS.gene56980:intron 45.0%
ATTGAGAGATACCCGGTTAC+TGG + chr5.1:10456019-10456038 MS.gene56980:CDS 45.0%
CATCAGTCAATATGTCCGAG+TGG - chr5.1:10455454-10455473 None:intergenic 45.0%
GAGGCTAAGAAGCCTAACAA+AGG - chr5.1:10454953-10454972 None:intergenic 45.0%
GTAGACAAGAACTTTGAGCC+AGG - chr5.1:10456094-10456113 None:intergenic 45.0%
GTATCCTAGCAAACCCCTTT+AGG + chr5.1:10455789-10455808 MS.gene56980:intron 45.0%
GTGTCTAACCATCCAGTAAC+CGG - chr5.1:10456034-10456053 None:intergenic 45.0%
TGTCTAACCATCCAGTAACC+GGG - chr5.1:10456033-10456052 None:intergenic 45.0%
! ATTTGCTAGAACACACCCAC+TGG + chr5.1:10455518-10455537 MS.gene56980:intron 45.0%
! CTAGTACATGGTGTTAGCGT+TGG + chr5.1:10454794-10454813 MS.gene56980:CDS 45.0%
!! CTTGTTGGACATAGCTTTGG+AGG + chr5.1:10454998-10455017 MS.gene56980:CDS 45.0%
ACACTTGACCTTGCAGCTAG+TGG + chr5.1:10454875-10454894 MS.gene56980:CDS 50.0%
AGAGATACCCGGTTACTGGA+TGG + chr5.1:10456023-10456042 MS.gene56980:CDS 50.0%
TGTTGATGCCACTAGCTGCA+AGG - chr5.1:10454886-10454905 None:intergenic 50.0%
! ATGGTGTTAGCGTTGGAGCT+TGG + chr5.1:10454801-10454820 MS.gene56980:CDS 50.0%
! TTAGCGTTGGAGCTTGGAGT+TGG + chr5.1:10454807-10454826 MS.gene56980:CDS 50.0%
AGCAGGGTTCGAACCTGCGC+GGG + chr5.1:10455394-10455413 MS.gene56980:intron 65.0%
CAGCAGGGTTCGAACCTGCG+CGG + chr5.1:10455393-10455412 MS.gene56980:intron 65.0%
CTGTTGGGCTTCGCCCGCGC+AGG - chr5.1:10455410-10455429 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 10454767 10456141 10454767 ID=MS.gene56980
chr5.1 mRNA 10454767 10456141 10454767 ID=MS.gene56980.t1;Parent=MS.gene56980
chr5.1 exon 10454767 10455132 10454767 ID=MS.gene56980.t1.exon1;Parent=MS.gene56980.t1
chr5.1 CDS 10454767 10455132 10454767 ID=cds.MS.gene56980.t1;Parent=MS.gene56980.t1
chr5.1 exon 10456016 10456141 10456016 ID=MS.gene56980.t1.exon2;Parent=MS.gene56980.t1
chr5.1 CDS 10456016 10456141 10456016 ID=cds.MS.gene56980.t1;Parent=MS.gene56980.t1
Gene Sequence

>MS.gene56980

ATGAACCAAAAACAGAAGCATTTTGTTCTAGTACATGGTGTTAGCGTTGGAGCTTGGAGTTGGTACAAGCTTAAACCACAGTTAGAATCTGTAGGTCACAAAGTCACAACACTTGACCTTGCAGCTAGTGGCATCAACACACACAAAATTGAAGATGTTCATACTTTTACTGAGTATGCTAAGCCTTTGTTAGGCTTCTTAGCCTCACTTGATCCAAATGAAAAAGTGGTTCTTGTTGGACATAGCTTTGGAGGAATGAGTATAGCTCTTGCAATGGAAAAATTTCCTGAAAAAATTGAAGTTGGAATTTTCCTAGCAGCTTTTATTCCTGATACTCAACACAAACCATCATATGTTCTAGAACAGGTGAGAATTTATTCTTTTTATATAACGTAGTACTTAAATATTAGATAGAATTATTGTCAATTAATTTGTGTAAGCTAATAGATTAGAATATTGTTAATTTTCTTAGTGGAAATAAGCATGTAATTGATTGTTTCAAACAAAAAAAAAGTATCTTTACCATCAAATGTAAACTAGGATAAGATGAGAATATTTTTGTCTTAAAAAATTGAATAAATATAGAAATGTGATATTTAGTTCTTTAAATTAAAAAAATACGTCAACAGCAGGGTTCGAACCTGCGCGGGCGAAGCCCAACAGATTTCAAGTCTGTCTCCTTAACCACTCGGACATATTGACTGATGATATTTGTTTTAAGTTGGTTAATATTTATTAGTTTAGTTATGGGATTTGCTAGAACACACCCACTGGTTTTTATGTGAGAGTGTTTTAGCAAGCTACACCTTAAGGTGTATTTAACTACTTTTTAGTTATATCTTACTAAAACACACCTTCTAAAAAATAAGTGGGTGTATCCTAGCAAATGCCTATAGGAGTTGCTAACATACACCCGTTTATTTTTTTAGAAGGTGTGTTTTAGCAATATTCACCTTAAGGTGTATTTAACAACTTTTTAGTTATATCTTTGCTAAAACACACCTTAATAAAAACTAGTGGGTGTATCCTAGCAAACCCCTTTAGGTATAATCACTATATAAAACATTGCTCATTGTAGTCAACTTTTCTCTTGATTTATTTCTTTTTTCTTTTTTTGATCTCTATCATGCTTCTCAACACAAATCAGATTTCTCATTAAAACCTTGATAAATTAGATTTATATTTCTTTGTATTAATGTTTATGTTTTATTTACCTGACCTGACTTGGATCATTTTGTGATATGGATAGTATATTGAGAGATACCCGGTTACTGGATGGTTAGACACTGAGTTTTCATTTGGTGGAAACAAAATGCTGTTGTTACCTGGCTCAAAGTTCTTGTCTACTAAGTTCTTTCAACTCTGCTCCATTGAG

Protein sequence

>MS.gene56980.t1

MNQKQKHFVLVHGVSVGAWSWYKLKPQLESVGHKVTTLDLAASGINTHKIEDVHTFTEYAKPLLGFLASLDPNEKVVLVGHSFGGMSIALAMEKFPEKIEVGIFLAAFIPDTQHKPSYVLEQYIERYPVTGWLDTEFSFGGNKMLLLPGSKFLSTKFFQLCSIE