AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055441


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055441.t1 MTR_5g005470 92.500 160 3 1 1 151 1 160 3.09e-105 299
MS.gene055441.t1 MTR_5g016200 73.125 160 31 2 1 151 1 157 3.55e-78 230
MS.gene055441.t1 MTR_5g016220 71.698 159 36 1 2 151 127 285 1.37e-77 233
MS.gene055441.t1 MTR_5g016220 74.803 127 31 1 1 127 1 126 1.44e-62 195
MS.gene055441.t1 MTR_6g088010 51.852 162 67 2 1 151 1 162 7.20e-50 159
MS.gene055441.t1 MTR_6g042230 51.250 160 68 2 1 151 1 159 4.86e-48 154
MS.gene055441.t1 MTR_6g088020 52.761 163 64 3 1 151 1 162 1.76e-47 153
MS.gene055441.t1 MTR_7g090220 31.788 151 91 3 10 150 53 201 3.66e-17 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055441.t1 AT3G22320 64.780 159 47 1 2 151 1 159 1.62e-71 214
MS.gene055441.t1 AT5G57980 36.250 160 89 4 5 151 5 164 2.13e-28 104
MS.gene055441.t1 AT3G57080 32.704 159 95 4 3 151 20 176 1.21e-18 79.3
MS.gene055441.t1 AT2G41340 33.544 158 93 4 4 151 17 172 6.01e-17 73.9
MS.gene055441.t1 AT2G41340 33.544 158 93 4 4 151 17 172 7.63e-17 73.9
MS.gene055441.t1 AT2G41340 33.544 158 93 4 4 151 17 172 1.44e-16 73.6
MS.gene055441.t1 AT3G54490 28.387 155 99 3 7 151 35 187 7.25e-11 58.5

Find 18 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTTCAGAAACACAAGTTT+TGG 0.351766 5.1:+13374072 None:intergenic
TTTGTCTTTGAAATCGTGTT+TGG 0.360361 5.1:+13374291 None:intergenic
AATTGCTTCCAAATTTCATT+TGG 0.361732 5.1:-13374053 MS.gene055441:CDS
ACACCGACTTTGGCTTCTTC+AGG 0.396133 5.1:+13374184 None:intergenic
AATAGCTCTATAAACATTCT+CGG 0.403161 5.1:+13374126 None:intergenic
GACAGTGTATCTCTCAAGTA+AGG 0.434087 5.1:+13373731 None:intergenic
CAATGAAGACTTACACCAAC+AGG 0.446390 5.1:-13374156 MS.gene055441:CDS
TCGTTGAGAATCTGATGTTC+CGG 0.447338 5.1:+13373768 None:intergenic
AATCACCGACGAGGTAGTTT+CGG 0.453235 5.1:+13374329 None:intergenic
AAAGAGCATGTGCCTTGTGC+CGG 0.514977 5.1:-13373787 MS.gene055441:intron
TTTGGCAAAGGGAGTAAGAC+TGG 0.523494 5.1:+13374090 None:intergenic
CACGATTTCAAAGACAAATA+CGG 0.530990 5.1:-13374286 MS.gene055441:CDS
GAATCTGATGTTCCGGCACA+AGG 0.545718 5.1:+13373775 None:intergenic
ATTCTCGGAATTCATCCTGT+TGG 0.579040 5.1:+13374141 None:intergenic
AAGACATAGATCTGATCAGA+AGG 0.597739 5.1:+13374211 None:intergenic
TTTCCTGAAGAAGCCAAAGT+CGG 0.608753 5.1:-13374187 MS.gene055441:CDS
AAAGACCGAAACTACCTCGT+CGG 0.681273 5.1:-13374334 MS.gene055441:CDS
TGAGTTCGAAATCACCGACG+AGG 0.765537 5.1:+13374320 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACTTCCAAATGAAATT+TGG + chr5.1:13374092-13374111 None:intergenic 20.0%
!!! TCATTTTCTCAAATTATCAT+CGG + chr5.1:13374209-13374228 None:intergenic 20.0%
! AATTGCTTCCAAATTTCATT+TGG - chr5.1:13374081-13374100 MS.gene055441:CDS 25.0%
! ATAAACCGCATAAACAAATT+AGG + chr5.1:13374144-13374163 None:intergenic 25.0%
! GTTATTTATACCTGACATAT+TGG - chr5.1:13374177-13374196 MS.gene055441:CDS 25.0%
! TAAACCGCATAAACAAATTA+GGG + chr5.1:13374143-13374162 None:intergenic 25.0%
!! AATAGCTCTATAAACATTCT+CGG + chr5.1:13374011-13374030 None:intergenic 25.0%
!! AATTTCAGAAACACAAGTTT+TGG + chr5.1:13374065-13374084 None:intergenic 25.0%
!! ATTTCATTTGGAAGTTTTTC+AGG - chr5.1:13374093-13374112 MS.gene055441:CDS 25.0%
!!! ATCAGAAGGTTTATCTTTTT+TGG + chr5.1:13373912-13373931 None:intergenic 25.0%
!!! TAAACCCTAATTTGTTTATG+CGG - chr5.1:13374136-13374155 MS.gene055441:CDS 25.0%
CACGATTTCAAAGACAAATA+CGG - chr5.1:13373848-13373867 MS.gene055441:intron 30.0%
!! TTTGTCTTTGAAATCGTGTT+TGG + chr5.1:13373846-13373865 None:intergenic 30.0%
AAGACATAGATCTGATCAGA+AGG + chr5.1:13373926-13373945 None:intergenic 35.0%
GCGGTTTATAAGAGTTAGTT+AGG - chr5.1:13374155-13374174 MS.gene055441:CDS 35.0%
! CTCAAGTAAGGTTTTCTTCT+CGG + chr5.1:13374394-13374413 None:intergenic 35.0%
!! AGAAACACAAGTTTTGGCAA+AGG + chr5.1:13374059-13374078 None:intergenic 35.0%
!! GAAACACAAGTTTTGGCAAA+GGG + chr5.1:13374058-13374077 None:intergenic 35.0%
ATTCTCGGAATTCATCCTGT+TGG + chr5.1:13373996-13374015 None:intergenic 40.0%
CAATGAAGACTTACACCAAC+AGG - chr5.1:13373978-13373997 MS.gene055441:intron 40.0%
TCGTTGAGAATCTGATGTTC+CGG + chr5.1:13374369-13374388 None:intergenic 40.0%
TGCTTCAGTATAACTGTTGC+TGG - chr5.1:13374281-13374300 MS.gene055441:CDS 40.0%
TTTCCTGAAGAAGCCAAAGT+CGG - chr5.1:13373947-13373966 MS.gene055441:intron 40.0%
! GCATTTGCATCACTGTTTTG+CGG + chr5.1:13373778-13373797 None:intergenic 40.0%
!!! CATTGTTTTCACACCGACTT+TGG + chr5.1:13373963-13373982 None:intergenic 40.0%
AAAGACCGAAACTACCTCGT+CGG - chr5.1:13373800-13373819 MS.gene055441:intron 45.0%
AATCACCGACGAGGTAGTTT+CGG + chr5.1:13373808-13373827 None:intergenic 45.0%
TTTGGCAAAGGGAGTAAGAC+TGG + chr5.1:13374047-13374066 None:intergenic 45.0%
!! TCGGTGTTGACCAATATGTC+AGG + chr5.1:13374190-13374209 None:intergenic 45.0%
ATGTGTCCTTCTAGTCCGAG+AGG - chr5.1:13374246-13374265 MS.gene055441:CDS 50.0%
GAATCTGATGTTCCGGCACA+AGG + chr5.1:13374362-13374381 None:intergenic 50.0%
TGAGTTCGAAATCACCGACG+AGG + chr5.1:13373817-13373836 None:intergenic 50.0%
! AAAGAGCATGTGCCTTGTGC+CGG - chr5.1:13374347-13374366 MS.gene055441:CDS 50.0%
!! ACACCGACTTTGGCTTCTTC+AGG + chr5.1:13373953-13373972 None:intergenic 50.0%
GCACAAGCACAGACACCTCT+CGG + chr5.1:13374264-13374283 None:intergenic 55.0%
TGGTGTGTGTGTGTGTGTGC+AGG - chr5.1:13374301-13374320 MS.gene055441:CDS 55.0%
TGTGTGTGTGTGTGTGCAGG+AGG - chr5.1:13374304-13374323 MS.gene055441:CDS 55.0%
! CAGACACCTCTCGGACTAGA+AGG + chr5.1:13374255-13374274 None:intergenic 55.0%
GTGTGTGTGTGTGCAGGAGG+CGG - chr5.1:13374307-13374326 MS.gene055441:CDS 60.0%
TGTGCAGGAGGCGGAGTTGC+TGG - chr5.1:13374316-13374335 MS.gene055441:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 13373734 13374422 13373734 ID=MS.gene055441
chr5.1 mRNA 13373734 13374422 13373734 ID=MS.gene055441.t1;Parent=MS.gene055441
chr5.1 exon 13374042 13374422 13374042 ID=MS.gene055441.t1.exon1;Parent=MS.gene055441.t1
chr5.1 CDS 13374042 13374422 13374042 ID=cds.MS.gene055441.t1;Parent=MS.gene055441.t1
chr5.1 exon 13373734 13373805 13373734 ID=MS.gene055441.t1.exon2;Parent=MS.gene055441.t1
chr5.1 CDS 13373734 13373805 13373734 ID=cds.MS.gene055441.t1;Parent=MS.gene055441.t1
Gene Sequence

>MS.gene055441

ATGGTGTTTTCAGAGGAAGAAATAACGCGACTATACAGAATCCGCAAAACAGTGATGCAAATGCTGAAAGACCGAAACTACCTCGTCGGTGATTTCGAACTCAACATGTCCAAACACGATTTCAAAGACAAATACGGCGAGAATATGAAGAGAGAAGATCTTGTTATTAACAAAACCAAAAAAGATAAACCTTCTGATCAGATCTATGTCTTCTTTCCTGAAGAAGCCAAAGTCGGTGTGAAAACAATGAAGACTTACACCAACAGGATGAATTCCGAGAATGTTTATAGAGCTATTCTTGTTTGTCAGACCAGTCTTACTCCCTTTGCCAAAACTTGTGTTTCTGAAATTGCTTCCAAATTTCATTTGGAAGTTTTTCAGGTAATTCTTTTGTTTGTTTTATAAACCCTAATTTGTTTATGCGGTTTATAAGAGTTAGTTAGGTTATTTATACCTGACATATTGGTCAACACCGATGATAATTTGAGAAAATGAATTAATTGAATGTAATTATGTGTCCTTCTAGTCCGAGAGGTGTCTGTGCTTGTGCTTCAGTATAACTGTTGCTGGTGTGTGTGTGTGTGTGCAGGAGGCGGAGTTGCTGGTTAATATAAAAGAGCATGTGCCTTGTGCCGGAACATCAGATTCTCAACGACACCGAGAAGAAAACCTTACTTGAGAGATACACT

Protein sequence

>MS.gene055441.t1

MVFSEEEITRLYRIRKTVMQMLKDRNYLVGDFELNMSKHDFKDKYGENMKREDLVINKTKKDKPSDQIYVFFPEEAKVGVKTMKTYTNRMNSENVYRAILVCQTSLTPFAKTCVSEIASKFHLEVFQSMCLVPEHQILNDTEKKTLLERYT