Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57982.t1 | XP_003609483.1 | 96.8 | 158 | 5 | 0 | 1 | 158 | 308 | 465 | 4.10E-81 | 310.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57982.t1 | Q9SZ45 | 63.0 | 138 | 51 | 0 | 1 | 138 | 333 | 470 | 8.7e-48 | 191.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57982.t1 | G7JGM0 | 96.8 | 158 | 5 | 0 | 1 | 158 | 308 | 465 | 3.0e-81 | 310.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene57982 | 0.849121 | 3.96E-60 | -1.69E-46 |
| MS.gene049910 | MS.gene57982 | 0.810886 | 9.22E-51 | -1.69E-46 |
| MS.gene049915 | MS.gene57982 | 0.800436 | 1.44E-48 | -1.69E-46 |
| MS.gene050301 | MS.gene57982 | 0.803882 | 2.82E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene57982 | 0.809351 | 1.97E-50 | -1.69E-46 |
| MS.gene052077 | MS.gene57982 | 0.809248 | 2.08E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene57982 | 0.828765 | 7.49E-55 | -1.69E-46 |
| MS.gene054184 | MS.gene57982 | 0.826689 | 2.36E-54 | -1.69E-46 |
| MS.gene054185 | MS.gene57982 | 0.833119 | 6.40E-56 | -1.69E-46 |
| MS.gene054930 | MS.gene57982 | 0.803795 | 2.94E-49 | -1.69E-46 |
| MS.gene055133 | MS.gene57982 | 0.802806 | 4.71E-49 | -1.69E-46 |
| MS.gene055422 | MS.gene57982 | 0.824521 | 7.72E-54 | -1.69E-46 |
| MS.gene057439 | MS.gene57982 | 0.80205 | 6.74E-49 | -1.69E-46 |
| MS.gene057754 | MS.gene57982 | 0.831086 | 2.04E-55 | -1.69E-46 |
| MS.gene059124 | MS.gene57982 | 0.802455 | 5.57E-49 | -1.69E-46 |
| MS.gene059597 | MS.gene57982 | 0.802674 | 5.02E-49 | -1.69E-46 |
| MS.gene05984 | MS.gene57982 | 0.808059 | 3.73E-50 | -1.69E-46 |
| MS.gene060476 | MS.gene57982 | 0.853345 | 2.54E-61 | -1.69E-46 |
| MS.gene061049 | MS.gene57982 | 0.834201 | 3.44E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57982.t1 | MTR_4g116190 | 96.835 | 158 | 5 | 0 | 1 | 158 | 308 | 465 | 8.36e-107 | 314 |
| MS.gene57982.t1 | MTR_1g012850 | 61.635 | 159 | 58 | 2 | 1 | 156 | 295 | 453 | 2.46e-63 | 202 |
| MS.gene57982.t1 | MTR_3g106550 | 60.625 | 160 | 60 | 2 | 1 | 157 | 286 | 445 | 6.08e-61 | 196 |
| MS.gene57982.t1 | MTR_3g106550 | 69.000 | 100 | 31 | 0 | 1 | 100 | 286 | 385 | 2.65e-39 | 138 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57982.t1 | AT4G32060 | 63.043 | 138 | 51 | 0 | 1 | 138 | 333 | 470 | 6.67e-60 | 194 |
| MS.gene57982.t1 | AT4G32060 | 63.725 | 102 | 37 | 0 | 1 | 102 | 333 | 434 | 8.69e-40 | 140 |
Find 32 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGTTATGGAAAAGTTAA+TGG | 0.221348 | 8.3:+9947446 | MS.gene57982:CDS |
| GAAGACATTTCCTCACGTTT+TGG | 0.303320 | 8.3:+9947732 | MS.gene57982:CDS |
| AGAAGGGTGGTATATGCATT+TGG | 0.312703 | 8.3:+9947844 | MS.gene57982:CDS |
| TGCAAAATCCTTGGCCGAAA+TGG | 0.315325 | 8.3:-9947258 | None:intergenic |
| CCCTATCTTGAATCTGCATT+TGG | 0.315756 | 8.3:-9947892 | None:intergenic |
| GAAGGGTGGTATATGCATTT+GGG | 0.347349 | 8.3:+9947845 | MS.gene57982:CDS |
| ACCAAATGCAGATTCAAGAT+AGG | 0.395746 | 8.3:+9947891 | MS.gene57982:CDS |
| CAATAAAAGAGGAACCATTT+CGG | 0.419212 | 8.3:+9947244 | MS.gene57982:CDS |
| TCTCTGGCCATATTCAGTTA+TGG | 0.434138 | 8.3:+9947434 | MS.gene57982:CDS |
| TGGAAAAGTTAATGGAGTAT+TGG | 0.435781 | 8.3:+9947454 | MS.gene57982:CDS |
| ACATAAACAAACTGCTGGAT+AGG | 0.437978 | 8.3:+9947312 | MS.gene57982:CDS |
| TAATCACATAAACAAACTGC+TGG | 0.470159 | 8.3:+9947307 | MS.gene57982:CDS |
| CAAGGATAGTGCAAAATCCT+TGG | 0.474280 | 8.3:-9947267 | None:intergenic |
| AGACAAAAGAATTACGTTTG+AGG | 0.485400 | 8.3:+9947367 | MS.gene57982:CDS |
| AAGAGGAACCATTTCGGCCA+AGG | 0.487222 | 8.3:+9947250 | MS.gene57982:CDS |
| CACTATGATCACAATAAAAG+AGG | 0.488832 | 8.3:+9947233 | MS.gene57982:CDS |
| TGTTGGTTGAACTGTGTAAA+GGG | 0.494222 | 8.3:+9947774 | MS.gene57982:CDS |
| ACCTCTCAGAAACTAAAAGA+AGG | 0.495468 | 8.3:+9947827 | MS.gene57982:intron |
| GAAGTTGCAGTCCTTCTCTC+TGG | 0.500761 | 8.3:+9947418 | MS.gene57982:CDS |
| GTCTTTGATGCTAACCGTGA+TGG | 0.529666 | 8.3:+9947666 | MS.gene57982:CDS |
| TTGGGAATTTGATAACATGT+TGG | 0.531352 | 8.3:+9947757 | MS.gene57982:CDS |
| CCAAATGCAGATTCAAGATA+GGG | 0.535702 | 8.3:+9947892 | MS.gene57982:CDS |
| CATAAACAAACTGCTGGATA+GGG | 0.559819 | 8.3:+9947313 | MS.gene57982:CDS |
| ATGTTGGTTGAACTGTGTAA+AGG | 0.593116 | 8.3:+9947773 | MS.gene57982:CDS |
| ACTGAATATGGCCAGAGAGA+AGG | 0.593953 | 8.3:-9947429 | None:intergenic |
| CCTCTCAGAAACTAAAAGAA+GGG | 0.603062 | 8.3:+9947828 | MS.gene57982:intron |
| TCTTTGATGCTAACCGTGAT+GGG | 0.618328 | 8.3:+9947667 | MS.gene57982:CDS |
| CTCAGAAACTAAAAGAAGGG+TGG | 0.628738 | 8.3:+9947831 | MS.gene57982:intron |
| ATTCCCAAATCCAAAACGTG+AGG | 0.644135 | 8.3:-9947742 | None:intergenic |
| AATGTCTGCAGATGCAACCA+AGG | 0.647906 | 8.3:-9947285 | None:intergenic |
| TTGCACTTAAGCTCCCATCA+CGG | 0.666778 | 8.3:-9947680 | None:intergenic |
| TGTAATGTCATGTAGGTATG+CGG | 0.675183 | 8.3:+9947603 | MS.gene57982:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGATGAATAACTTATTATTA+CGG | + | chr8.3:9947575-9947594 | MS.gene57982:intron | 15.0% |
| !!! | AATATATTTTTAGGCATAAT+AGG | - | chr8.3:9947525-9947544 | None:intergenic | 15.0% |
| !!! | ACTACTGTAAATATATTTTT+AGG | - | chr8.3:9947534-9947553 | None:intergenic | 15.0% |
| !!! | ATATATTTTTAGGCATAATA+GGG | - | chr8.3:9947524-9947543 | None:intergenic | 15.0% |
| ! | TTCAGTTATGGAAAAGTTAA+TGG | + | chr8.3:9947446-9947465 | MS.gene57982:CDS | 25.0% |
| !! | AACTTTTCCATAACTGAATA+TGG | - | chr8.3:9947444-9947463 | None:intergenic | 25.0% |
| !! | ATTCTTTTGTCTCTTATATG+TGG | - | chr8.3:9947359-9947378 | None:intergenic | 25.0% |
| AGACAAAAGAATTACGTTTG+AGG | + | chr8.3:9947367-9947386 | MS.gene57982:CDS | 30.0% | |
| CAATAAAAGAGGAACCATTT+CGG | + | chr8.3:9947244-9947263 | MS.gene57982:CDS | 30.0% | |
| CACTATGATCACAATAAAAG+AGG | + | chr8.3:9947233-9947252 | MS.gene57982:CDS | 30.0% | |
| TAATCACATAAACAAACTGC+TGG | + | chr8.3:9947307-9947326 | MS.gene57982:CDS | 30.0% | |
| TGGAAAAGTTAATGGAGTAT+TGG | + | chr8.3:9947454-9947473 | MS.gene57982:CDS | 30.0% | |
| ! | TTGGGAATTTGATAACATGT+TGG | + | chr8.3:9947757-9947776 | MS.gene57982:CDS | 30.0% |
| ACATAAACAAACTGCTGGAT+AGG | + | chr8.3:9947312-9947331 | MS.gene57982:CDS | 35.0% | |
| ACCTCTCAGAAACTAAAAGA+AGG | + | chr8.3:9947827-9947846 | MS.gene57982:intron | 35.0% | |
| CATAAACAAACTGCTGGATA+GGG | + | chr8.3:9947313-9947332 | MS.gene57982:CDS | 35.0% | |
| CCTCTCAGAAACTAAAAGAA+GGG | + | chr8.3:9947828-9947847 | MS.gene57982:intron | 35.0% | |
| GGTATCATGTAATGTCATGT+AGG | + | chr8.3:9947596-9947615 | MS.gene57982:intron | 35.0% | |
| TGTAATGTCATGTAGGTATG+CGG | + | chr8.3:9947603-9947622 | MS.gene57982:intron | 35.0% | |
| ! | ATGTTGGTTGAACTGTGTAA+AGG | + | chr8.3:9947773-9947792 | MS.gene57982:CDS | 35.0% |
| ! | TAGTTTCTGAGAGGTGAAAA+TGG | - | chr8.3:9947822-9947841 | None:intergenic | 35.0% |
| ! | TGTTGGTTGAACTGTGTAAA+GGG | + | chr8.3:9947774-9947793 | MS.gene57982:CDS | 35.0% |
| !! | ATTTCCTCACGTTTTGGATT+TGG | + | chr8.3:9947738-9947757 | MS.gene57982:CDS | 35.0% |
| !! | GCATTTGGGTTTTACAGTAA+TGG | + | chr8.3:9947859-9947878 | MS.gene57982:CDS | 35.0% |
| !! | TTTCCTCACGTTTTGGATTT+GGG | + | chr8.3:9947739-9947758 | MS.gene57982:CDS | 35.0% |
| AGAAGGGTGGTATATGCATT+TGG | + | chr8.3:9947844-9947863 | MS.gene57982:CDS | 40.0% | |
| ATTCCCAAATCCAAAACGTG+AGG | - | chr8.3:9947745-9947764 | None:intergenic | 40.0% | |
| CAAGGATAGTGCAAAATCCT+TGG | - | chr8.3:9947270-9947289 | None:intergenic | 40.0% | |
| CTCAGAAACTAAAAGAAGGG+TGG | + | chr8.3:9947831-9947850 | MS.gene57982:intron | 40.0% | |
| GAAGGGTGGTATATGCATTT+GGG | + | chr8.3:9947845-9947864 | MS.gene57982:CDS | 40.0% | |
| TCTCTGGCCATATTCAGTTA+TGG | + | chr8.3:9947434-9947453 | MS.gene57982:CDS | 40.0% | |
| TCTTTGATGCTAACCGTGAT+GGG | + | chr8.3:9947667-9947686 | MS.gene57982:CDS | 40.0% | |
| ! | CAAGGATTTTGCACTATCCT+TGG | + | chr8.3:9947268-9947287 | MS.gene57982:CDS | 40.0% |
| ! | GAAGACATTTCCTCACGTTT+TGG | + | chr8.3:9947732-9947751 | MS.gene57982:CDS | 40.0% |
| ! | TGAGGAGTTCAAAGCTTTTG+CGG | + | chr8.3:9947385-9947404 | MS.gene57982:CDS | 40.0% |
| ! | TTTTCAAAGAGCAGCATCTC+AGG | + | chr8.3:9947487-9947506 | MS.gene57982:CDS | 40.0% |
| !!! | CCCTTCTTTTAGTTTCTGAG+AGG | - | chr8.3:9947831-9947850 | None:intergenic | 40.0% |
| AATGTCTGCAGATGCAACCA+AGG | - | chr8.3:9947288-9947307 | None:intergenic | 45.0% | |
| ACTGAATATGGCCAGAGAGA+AGG | - | chr8.3:9947432-9947451 | None:intergenic | 45.0% | |
| GTCTTTGATGCTAACCGTGA+TGG | + | chr8.3:9947666-9947685 | MS.gene57982:CDS | 45.0% | |
| TGCAAAATCCTTGGCCGAAA+TGG | - | chr8.3:9947261-9947280 | None:intergenic | 45.0% | |
| TTGCACTTAAGCTCCCATCA+CGG | - | chr8.3:9947683-9947702 | None:intergenic | 45.0% | |
| AAGAGGAACCATTTCGGCCA+AGG | + | chr8.3:9947250-9947269 | MS.gene57982:CDS | 50.0% | |
| GAAGTTGCAGTCCTTCTCTC+TGG | + | chr8.3:9947418-9947437 | MS.gene57982:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 9947212 | 9947912 | 9947212 | ID=MS.gene57982 |
| chr8.3 | mRNA | 9947212 | 9947912 | 9947212 | ID=MS.gene57982.t1;Parent=MS.gene57982 |
| chr8.3 | exon | 9947212 | 9947508 | 9947212 | ID=MS.gene57982.t1.exon1;Parent=MS.gene57982.t1 |
| chr8.3 | CDS | 9947212 | 9947508 | 9947212 | ID=cds.MS.gene57982.t1;Parent=MS.gene57982.t1 |
| chr8.3 | exon | 9947618 | 9947795 | 9947618 | ID=MS.gene57982.t1.exon2;Parent=MS.gene57982.t1 |
| chr8.3 | CDS | 9947618 | 9947795 | 9947618 | ID=cds.MS.gene57982.t1;Parent=MS.gene57982.t1 |
| chr8.3 | exon | 9947836 | 9947912 | 9947836 | ID=MS.gene57982.t1.exon3;Parent=MS.gene57982.t1 |
| chr8.3 | CDS | 9947836 | 9947912 | 9947836 | ID=cds.MS.gene57982.t1;Parent=MS.gene57982.t1 |
| Gene Sequence |
| Protein sequence |
