Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58229.t1 | RHN64134.1 | 96 | 50 | 2 | 0 | 1 | 50 | 1 | 50 | 1.50E-19 | 105.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58229.t1 | A0A072V269 | 96.0 | 50 | 2 | 0 | 1 | 50 | 1 | 50 | 1.1e-19 | 105.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene58229 | -0.810693 | 1.02E-50 | -1.69E-46 |
| MS.gene049320 | MS.gene58229 | -0.810182 | 1.31E-50 | -1.69E-46 |
| MS.gene050504 | MS.gene58229 | -0.801067 | 1.07E-48 | -1.69E-46 |
| MS.gene050577 | MS.gene58229 | -0.822637 | 2.13E-53 | -1.69E-46 |
| MS.gene050612 | MS.gene58229 | -0.80784 | 4.15E-50 | -1.69E-46 |
| MS.gene050699 | MS.gene58229 | -0.812796 | 3.54E-51 | -1.69E-46 |
| MS.gene051651 | MS.gene58229 | 0.81663 | 5.01E-52 | -1.69E-46 |
| MS.gene05470 | MS.gene58229 | -0.850816 | 1.33E-60 | -1.69E-46 |
| MS.gene054954 | MS.gene58229 | -0.806122 | 9.59E-50 | -1.69E-46 |
| MS.gene055362 | MS.gene58229 | -0.819811 | 9.55E-53 | -1.69E-46 |
| MS.gene05589 | MS.gene58229 | -0.82438 | 8.33E-54 | -1.69E-46 |
| MS.gene056504 | MS.gene58229 | -0.818849 | 1.58E-52 | -1.69E-46 |
| MS.gene058334 | MS.gene58229 | 0.80822 | 3.45E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene58229 | -0.813182 | 2.91E-51 | -1.69E-46 |
| MS.gene058838 | MS.gene58229 | -0.805676 | 1.19E-49 | -1.69E-46 |
| MS.gene059022 | MS.gene58229 | -0.808773 | 2.63E-50 | -1.69E-46 |
| MS.gene059677 | MS.gene58229 | -0.819631 | 1.05E-52 | -1.69E-46 |
| MS.gene060405 | MS.gene58229 | 0.808701 | 2.72E-50 | -1.69E-46 |
| MS.gene060762 | MS.gene58229 | -0.807688 | 4.47E-50 | -1.69E-46 |
| MS.gene06077 | MS.gene58229 | -0.817934 | 2.55E-52 | -1.69E-46 |
| MS.gene061044 | MS.gene58229 | 0.813385 | 2.63E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58229.t1 | MTR_4g117450 | 96.000 | 50 | 2 | 0 | 1 | 50 | 1 | 50 | 1.09e-28 | 102 |
| MS.gene58229.t1 | MTR_4g117450 | 95.918 | 49 | 2 | 0 | 1 | 49 | 1 | 49 | 1.11e-28 | 103 |
| MS.gene58229.t1 | MTR_4g117400 | 78.000 | 50 | 11 | 0 | 1 | 50 | 55 | 104 | 2.35e-22 | 87.4 |
| MS.gene58229.t1 | MTR_4g117380 | 53.448 | 58 | 27 | 0 | 1 | 58 | 1 | 58 | 6.50e-17 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58229.t1 | AT3G23600 | 62.000 | 50 | 19 | 0 | 1 | 50 | 1 | 50 | 1.31e-16 | 71.6 |
| MS.gene58229.t1 | AT3G23600 | 56.140 | 57 | 25 | 0 | 1 | 57 | 1 | 57 | 1.34e-16 | 71.2 |
Find 27 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGACAAGGTTGCAGCAAC+CGG | 0.418523 | 8.3:-8770621 | MS.gene58229:CDS |
| CTGAACTCCTTCAGTGGTGC+TGG | 0.438411 | 8.3:-8771099 | MS.gene58229:CDS |
| GGTGTTGATGCTTATCTCAC+TGG | 0.438465 | 8.3:-8771057 | MS.gene58229:CDS |
| CCACTGAAGGAGTTCAGAAC+TGG | 0.500926 | 8.3:+8771105 | None:intergenic |
| GCTGGCCATGTTGCTAAACT+TGG | 0.501919 | 8.3:-8771081 | MS.gene58229:CDS |
| GGATCGCCGTTGAAGAAGTC+GGG | 0.513477 | 8.3:+8770582 | None:intergenic |
| AATGTCGGAGACGAGGAGAA+TGG | 0.515388 | 8.3:+8771014 | None:intergenic |
| GGCCATGTTGCTAAACTTGG+TGG | 0.517313 | 8.3:-8771078 | MS.gene58229:CDS |
| GTGAAGGATGTTTGCAGACA+AGG | 0.524084 | 8.3:-8770634 | MS.gene58229:intron |
| GTGGTTCCCGACTTCTTCAA+CGG | 0.543789 | 8.3:-8770588 | MS.gene58229:CDS |
| GGCGGGTTTGAGCAGCATGC+CGG | 0.559592 | 8.3:+8771126 | None:intergenic |
| GGGAACCACCACATAATATC+CGG | 0.564070 | 8.3:+8770602 | None:intergenic |
| TACCGAAAATGTCGGAGACG+AGG | 0.570501 | 8.3:+8771007 | None:intergenic |
| GTCAACATTCTGAGGATCGT+AGG | 0.572570 | 8.3:+8770560 | None:intergenic |
| GGGATCGCCGTTGAAGAAGT+CGG | 0.584664 | 8.3:+8770581 | None:intergenic |
| TCAACATTCTGAGGATCGTA+GGG | 0.585841 | 8.3:+8770561 | None:intergenic |
| CTGAAGGAGTTCAGAACTGG+CGG | 0.590785 | 8.3:+8771108 | None:intergenic |
| ATAGGTCTGTCAACATTCTG+AGG | 0.591096 | 8.3:+8770552 | None:intergenic |
| TGCAGCAACCGGATATTATG+TGG | 0.592827 | 8.3:-8770610 | MS.gene58229:CDS |
| CCAGTTCTGAACTCCTTCAG+TGG | 0.601307 | 8.3:-8771105 | MS.gene58229:CDS |
| AACATGGCCAGCACCACTGA+AGG | 0.605814 | 8.3:+8771092 | None:intergenic |
| CACCACCAAGTTTAGCAACA+TGG | 0.606489 | 8.3:+8771076 | None:intergenic |
| TGAAGGAGTTCAGAACTGGC+GGG | 0.608726 | 8.3:+8771109 | None:intergenic |
| GGAGACGAGGAGAATGGCGA+AGG | 0.615322 | 8.3:+8771020 | None:intergenic |
| TAGAACAGTACCGAAAATGT+CGG | 0.648160 | 8.3:+8770999 | None:intergenic |
| AGAATGGCGAAGGTGCAAAG+AGG | 0.653955 | 8.3:+8771030 | None:intergenic |
| AGCAACCGGATATTATGTGG+TGG | 0.672628 | 8.3:-8770607 | MS.gene58229:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATATAGATTTTGACTTTT+TGG | - | chr8.3:8770945-8770964 | MS.gene58229:intron | 15.0% |
| !!! | TTTTTCACTTACAAAAGAAT+TGG | - | chr8.3:8770985-8771004 | MS.gene58229:intron | 20.0% |
| ! | ATCATCATACCTTAAAATTG+GGG | + | chr8.3:8770859-8770878 | None:intergenic | 25.0% |
| ! | CATCATCATACCTTAAAATT+GGG | + | chr8.3:8770860-8770879 | None:intergenic | 25.0% |
| ! | TCAACAAAATGTAAACCATA+AGG | + | chr8.3:8771024-8771043 | None:intergenic | 25.0% |
| ! | TCATCATCATACCTTAAAAT+TGG | + | chr8.3:8770861-8770880 | None:intergenic | 25.0% |
| !! | AAATCTATATTTAAGACCAG+AGG | + | chr8.3:8770937-8770956 | None:intergenic | 25.0% |
| !! | AATCTATATTTAAGACCAGA+GGG | + | chr8.3:8770936-8770955 | None:intergenic | 25.0% |
| !!! | ACATGCTTAATTTGTTTTGT+AGG | - | chr8.3:8770825-8770844 | MS.gene58229:intron | 25.0% |
| CATATTGAAAACGTGAGAAT+TGG | + | chr8.3:8770889-8770908 | None:intergenic | 30.0% | |
| ! | GATATAAAGCCCCAATTTTA+AGG | - | chr8.3:8770847-8770866 | MS.gene58229:intron | 30.0% |
| ! | GGTTGTTTCAATATTCCTTA+TGG | - | chr8.3:8771006-8771025 | MS.gene58229:intron | 30.0% |
| ! | TTTCCTTCTCATGTATTTTG+CGG | - | chr8.3:8770714-8770733 | MS.gene58229:intron | 30.0% |
| ! | TTTTGTTGATCTGTTTGTGA+AGG | - | chr8.3:8771035-8771054 | MS.gene58229:CDS | 30.0% |
| GCAAAATACATGAGAAGGAA+AGG | + | chr8.3:8770715-8770734 | None:intergenic | 35.0% | |
| ! | TAGAACAGTACCGAAAATGT+CGG | + | chr8.3:8770689-8770708 | None:intergenic | 35.0% |
| ATAGGTCTGTCAACATTCTG+AGG | + | chr8.3:8771136-8771155 | None:intergenic | 40.0% | |
| ! | TCAACATTCTGAGGATCGTA+GGG | + | chr8.3:8771127-8771146 | None:intergenic | 40.0% |
| AGCAACCGGATATTATGTGG+TGG | - | chr8.3:8771078-8771097 | MS.gene58229:CDS | 45.0% | |
| CACCACCAAGTTTAGCAACA+TGG | + | chr8.3:8770612-8770631 | None:intergenic | 45.0% | |
| CCTCCGCAAAATACATGAGA+AGG | + | chr8.3:8770720-8770739 | None:intergenic | 45.0% | |
| GGGAACCACCACATAATATC+CGG | + | chr8.3:8771086-8771105 | None:intergenic | 45.0% | |
| GTGAAGGATGTTTGCAGACA+AGG | - | chr8.3:8771051-8771070 | MS.gene58229:CDS | 45.0% | |
| TGCAGCAACCGGATATTATG+TGG | - | chr8.3:8771075-8771094 | MS.gene58229:CDS | 45.0% | |
| TTGATACTAAACGCTCCCTC+TGG | - | chr8.3:8770918-8770937 | MS.gene58229:intron | 45.0% | |
| ! | CATGTATTTTGCGGAGGTCA+TGG | - | chr8.3:8770723-8770742 | MS.gene58229:intron | 45.0% |
| ! | CCTTCTCATGTATTTTGCGG+AGG | - | chr8.3:8770717-8770736 | MS.gene58229:intron | 45.0% |
| ! | GTCAACATTCTGAGGATCGT+AGG | + | chr8.3:8771128-8771147 | None:intergenic | 45.0% |
| !! | GGTGTTGATGCTTATCTCAC+TGG | - | chr8.3:8770628-8770647 | MS.gene58229:CDS | 45.0% |
| AATGTCGGAGACGAGGAGAA+TGG | + | chr8.3:8770674-8770693 | None:intergenic | 50.0% | |
| CCACTGAAGGAGTTCAGAAC+TGG | + | chr8.3:8770583-8770602 | None:intergenic | 50.0% | |
| CTGAAGGAGTTCAGAACTGG+CGG | + | chr8.3:8770580-8770599 | None:intergenic | 50.0% | |
| GCTGGCCATGTTGCTAAACT+TGG | - | chr8.3:8770604-8770623 | MS.gene58229:CDS | 50.0% | |
| GGCCATGTTGCTAAACTTGG+TGG | - | chr8.3:8770607-8770626 | MS.gene58229:CDS | 50.0% | |
| GTGGTTCCCGACTTCTTCAA+CGG | - | chr8.3:8771097-8771116 | MS.gene58229:CDS | 50.0% | |
| TACCGAAAATGTCGGAGACG+AGG | + | chr8.3:8770681-8770700 | None:intergenic | 50.0% | |
| TGAAGGAGTTCAGAACTGGC+GGG | + | chr8.3:8770579-8770598 | None:intergenic | 50.0% | |
| ! | AGAATGGCGAAGGTGCAAAG+AGG | + | chr8.3:8770658-8770677 | None:intergenic | 50.0% |
| ! | CTCCTCGTCTCCGACATTTT+CGG | - | chr8.3:8770676-8770695 | MS.gene58229:intron | 50.0% |
| !! | CCAGTTCTGAACTCCTTCAG+TGG | - | chr8.3:8770580-8770599 | MS.gene58229:CDS | 50.0% |
| AACATGGCCAGCACCACTGA+AGG | + | chr8.3:8770596-8770615 | None:intergenic | 55.0% | |
| CTGAACTCCTTCAGTGGTGC+TGG | - | chr8.3:8770586-8770605 | MS.gene58229:CDS | 55.0% | |
| GCAGACAAGGTTGCAGCAAC+CGG | - | chr8.3:8771064-8771083 | MS.gene58229:CDS | 55.0% | |
| GGATCGCCGTTGAAGAAGTC+GGG | + | chr8.3:8771106-8771125 | None:intergenic | 55.0% | |
| GGGATCGCCGTTGAAGAAGT+CGG | + | chr8.3:8771107-8771126 | None:intergenic | 55.0% | |
| GGAGACGAGGAGAATGGCGA+AGG | + | chr8.3:8770668-8770687 | None:intergenic | 60.0% | |
| ! | GGCGGGTTTGAGCAGCATGC+CGG | + | chr8.3:8770562-8770581 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 8770550 | 8771157 | 8770550 | ID=MS.gene58229 |
| chr8.3 | mRNA | 8770550 | 8771157 | 8770550 | ID=MS.gene58229.t1;Parent=MS.gene58229 |
| chr8.3 | exon | 8771010 | 8771157 | 8771010 | ID=MS.gene58229.t1.exon1;Parent=MS.gene58229.t1 |
| chr8.3 | CDS | 8771010 | 8771157 | 8771010 | ID=cds.MS.gene58229.t1;Parent=MS.gene58229.t1 |
| chr8.3 | exon | 8770550 | 8770650 | 8770550 | ID=MS.gene58229.t1.exon2;Parent=MS.gene58229.t1 |
| chr8.3 | CDS | 8770550 | 8770650 | 8770550 | ID=cds.MS.gene58229.t1;Parent=MS.gene58229.t1 |
| Gene Sequence |
| Protein sequence |
