Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59305.t1 | KEH17213.1 | 90.1 | 152 | 15 | 0 | 1 | 152 | 68 | 219 | 1.40E-45 | 192.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59305.t1 | A0A072TJZ5 | 90.1 | 152 | 15 | 0 | 1 | 152 | 68 | 219 | 9.7e-46 | 192.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050226 | MS.gene59305 | 0.806301 | 8.79E-50 | -1.69E-46 |
| MS.gene050227 | MS.gene59305 | 0.823586 | 1.28E-53 | -1.69E-46 |
| MS.gene050228 | MS.gene59305 | 0.814062 | 1.87E-51 | -1.69E-46 |
| MS.gene050442 | MS.gene59305 | 0.822487 | 2.31E-53 | -1.69E-46 |
| MS.gene050719 | MS.gene59305 | 0.829817 | 4.16E-55 | -1.69E-46 |
| MS.gene050721 | MS.gene59305 | 0.830181 | 3.39E-55 | -1.69E-46 |
| MS.gene050724 | MS.gene59305 | 0.824701 | 7.00E-54 | -1.69E-46 |
| MS.gene050824 | MS.gene59305 | 0.810513 | 1.11E-50 | -1.69E-46 |
| MS.gene052384 | MS.gene59305 | 0.817439 | 3.30E-52 | -1.69E-46 |
| MS.gene052896 | MS.gene59305 | 0.805527 | 1.28E-49 | -1.69E-46 |
| MS.gene054291 | MS.gene59305 | -0.810634 | 1.05E-50 | -1.69E-46 |
| MS.gene054467 | MS.gene59305 | 0.806412 | 8.33E-50 | -1.69E-46 |
| MS.gene054468 | MS.gene59305 | 0.821855 | 3.23E-53 | -1.69E-46 |
| MS.gene057980 | MS.gene59305 | 0.805629 | 1.22E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59305.t1 | MTR_0031s0130 | 91.447 | 152 | 13 | 0 | 1 | 152 | 68 | 219 | 6.05e-96 | 278 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAACCAGACCTTCCCTTTA+GGG | 0.251882 | 6.3:+63053155 | None:intergenic |
| GCAAACCAGACCTTCCCTTT+AGG | 0.271614 | 6.3:+63053154 | None:intergenic |
| TATCACCTAGAAGCTGGATA+TGG | 0.294596 | 6.3:-63053273 | MS.gene59305:CDS |
| TGTCCCCATATCCAGCTTCT+AGG | 0.304977 | 6.3:+63053268 | None:intergenic |
| AAGGCAACTAGGAGAAAATA+TGG | 0.390316 | 6.3:-63053004 | MS.gene59305:CDS |
| TCAGGATATTGTCGTGGCTT+GGG | 0.416808 | 6.3:-63053192 | MS.gene59305:CDS |
| ATCAGGATATTGTCGTGGCT+TGG | 0.440491 | 6.3:-63053193 | MS.gene59305:CDS |
| ATCACCTAGAAGCTGGATAT+GGG | 0.443128 | 6.3:-63053272 | MS.gene59305:CDS |
| TATTGTCGTGGCTTGGGAGC+TGG | 0.446927 | 6.3:-63053186 | MS.gene59305:CDS |
| AAACCCCTAAAGGGAAGGTC+TGG | 0.453319 | 6.3:-63053159 | MS.gene59305:CDS |
| TCAGTTGTGGGAGAGCGATC+AGG | 0.476415 | 6.3:-63053210 | MS.gene59305:CDS |
| AGCTGGAATTAAACCCCTAA+AGG | 0.483125 | 6.3:-63053169 | MS.gene59305:CDS |
| TTTGCAAGAACAATTAGAAA+AGG | 0.490497 | 6.3:-63053136 | MS.gene59305:CDS |
| TCTTCATATCACCTAGAAGC+TGG | 0.509469 | 6.3:-63053279 | MS.gene59305:CDS |
| GCTGGAATTAAACCCCTAAA+GGG | 0.516789 | 6.3:-63053168 | MS.gene59305:CDS |
| GAAGAGAGTCAAAGGCAACT+AGG | 0.523426 | 6.3:-63053015 | MS.gene59305:CDS |
| CAAAGTTTGAAGAGAGTCAA+AGG | 0.562523 | 6.3:-63053023 | MS.gene59305:CDS |
| AAACATGAGGGAAGAAATGA+CGG | 0.577928 | 6.3:-63053046 | MS.gene59305:CDS |
| AAACCAGACCTTCCCTTTAG+GGG | 0.599446 | 6.3:+63053156 | None:intergenic |
| GAATTAAACCCCTAAAGGGA+AGG | 0.614459 | 6.3:-63053164 | MS.gene59305:CDS |
| TCACCTAGAAGCTGGATATG+GGG | 0.621320 | 6.3:-63053271 | MS.gene59305:CDS |
| AAATATGGAGAAACAAGTTG+AGG | 0.626260 | 6.3:-63052989 | MS.gene59305:CDS |
| AATGTCTGAACTTGTTCACA+AGG | 0.634831 | 6.3:-63052764 | MS.gene59305:CDS |
| GAGCGATCAGGATATTGTCG+TGG | 0.667913 | 6.3:-63053198 | MS.gene59305:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTTTTAATATTTTTATA+GGG | - | chr6.3:63053212-63053231 | MS.gene59305:CDS | 0.0% |
| !!! | TTAATATTTTTATAGGGTAT+CGG | - | chr6.3:63053218-63053237 | MS.gene59305:CDS | 15.0% |
| !! | AGTATATAAATAACCATAGT+TGG | - | chr6.3:63053110-63053129 | MS.gene59305:CDS | 20.0% |
| !! | TGGTTATTTATATACTTACT+TGG | + | chr6.3:63053106-63053125 | None:intergenic | 20.0% |
| !!! | TTATATTTTTGTACCAACTA+TGG | + | chr6.3:63053126-63053145 | None:intergenic | 20.0% |
| ! | TTTGCAAGAACAATTAGAAA+AGG | - | chr6.3:63052896-63052915 | MS.gene59305:intron | 25.0% |
| !!! | ATTGCTTTTAAATCAGTTGT+GGG | - | chr6.3:63052810-63052829 | MS.gene59305:intron | 25.0% |
| !!! | TATTGCTTTTAAATCAGTTG+TGG | - | chr6.3:63052809-63052828 | MS.gene59305:intron | 25.0% |
| AAATATGGAGAAACAAGTTG+AGG | - | chr6.3:63053043-63053062 | MS.gene59305:CDS | 30.0% | |
| ! | GAAACAAGTTGAGGAAAAAA+TGG | - | chr6.3:63053052-63053071 | MS.gene59305:CDS | 30.0% |
| !!! | ATATTTTTATAGGGTATCGG+CGG | - | chr6.3:63053221-63053240 | MS.gene59305:CDS | 30.0% |
| AAACATGAGGGAAGAAATGA+CGG | - | chr6.3:63052986-63053005 | MS.gene59305:CDS | 35.0% | |
| AAGGCAACTAGGAGAAAATA+TGG | - | chr6.3:63053028-63053047 | MS.gene59305:CDS | 35.0% | |
| CAAAGTTTGAAGAGAGTCAA+AGG | - | chr6.3:63053009-63053028 | MS.gene59305:CDS | 35.0% | |
| CGAAAAAGAGAGAAACATGA+GGG | - | chr6.3:63052974-63052993 | MS.gene59305:CDS | 35.0% | |
| TCGAAAAAGAGAGAAACATG+AGG | - | chr6.3:63052973-63052992 | MS.gene59305:CDS | 35.0% | |
| ! | AATGTCTGAACTTGTTCACA+AGG | - | chr6.3:63053268-63053287 | MS.gene59305:CDS | 35.0% |
| AGCTGGAATTAAACCCCTAA+AGG | - | chr6.3:63052863-63052882 | MS.gene59305:intron | 40.0% | |
| ATCACCTAGAAGCTGGATAT+GGG | - | chr6.3:63052760-63052779 | MS.gene59305:CDS | 40.0% | |
| GAATTAAACCCCTAAAGGGA+AGG | - | chr6.3:63052868-63052887 | MS.gene59305:intron | 40.0% | |
| GCTGGAATTAAACCCCTAAA+GGG | - | chr6.3:63052864-63052883 | MS.gene59305:intron | 40.0% | |
| TATCACCTAGAAGCTGGATA+TGG | - | chr6.3:63052759-63052778 | MS.gene59305:CDS | 40.0% | |
| TCTTCATATCACCTAGAAGC+TGG | - | chr6.3:63052753-63052772 | MS.gene59305:CDS | 40.0% | |
| AAACCAGACCTTCCCTTTAG+GGG | + | chr6.3:63052879-63052898 | None:intergenic | 45.0% | |
| ATCAGGATATTGTCGTGGCT+TGG | - | chr6.3:63052839-63052858 | MS.gene59305:intron | 45.0% | |
| CAAACCAGACCTTCCCTTTA+GGG | + | chr6.3:63052880-63052899 | None:intergenic | 45.0% | |
| GAAGAGAGTCAAAGGCAACT+AGG | - | chr6.3:63053017-63053036 | MS.gene59305:CDS | 45.0% | |
| TCACCTAGAAGCTGGATATG+GGG | - | chr6.3:63052761-63052780 | MS.gene59305:CDS | 45.0% | |
| TCAGGATATTGTCGTGGCTT+GGG | - | chr6.3:63052840-63052859 | MS.gene59305:intron | 45.0% | |
| !!! | GTTATTTTTAATATTTTTAT+AGG | - | chr6.3:63053211-63053230 | MS.gene59305:CDS | 5.0% |
| !!! | TATTTTATTAAATTTGTTAT+GGG | - | chr6.3:63053187-63053206 | MS.gene59305:CDS | 5.0% |
| !!! | TTATTTTATTAAATTTGTTA+TGG | - | chr6.3:63053186-63053205 | MS.gene59305:CDS | 5.0% |
| AAACCCCTAAAGGGAAGGTC+TGG | - | chr6.3:63052873-63052892 | MS.gene59305:intron | 50.0% | |
| GAGCGATCAGGATATTGTCG+TGG | - | chr6.3:63052834-63052853 | MS.gene59305:intron | 50.0% | |
| GCAAACCAGACCTTCCCTTT+AGG | + | chr6.3:63052881-63052900 | None:intergenic | 50.0% | |
| TGTCCCCATATCCAGCTTCT+AGG | + | chr6.3:63052767-63052786 | None:intergenic | 50.0% | |
| TCAGTTGTGGGAGAGCGATC+AGG | - | chr6.3:63052822-63052841 | MS.gene59305:intron | 55.0% | |
| ! | TATTGTCGTGGCTTGGGAGC+TGG | - | chr6.3:63052846-63052865 | MS.gene59305:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 63052735 | 63053319 | 63052735 | ID=MS.gene59305 |
| chr6.3 | mRNA | 63052735 | 63053319 | 63052735 | ID=MS.gene59305.t1;Parent=MS.gene59305 |
| chr6.3 | exon | 63052948 | 63053319 | 63052948 | ID=MS.gene59305.t1.exon1;Parent=MS.gene59305.t1 |
| chr6.3 | CDS | 63052948 | 63053319 | 63052948 | ID=cds.MS.gene59305.t1;Parent=MS.gene59305.t1 |
| chr6.3 | exon | 63052735 | 63052821 | 63052735 | ID=MS.gene59305.t1.exon2;Parent=MS.gene59305.t1 |
| chr6.3 | CDS | 63052735 | 63052821 | 63052735 | ID=cds.MS.gene59305.t1;Parent=MS.gene59305.t1 |
| Gene Sequence |
| Protein sequence |
