Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59442.t1 | XP_003612131.2 | 97.9 | 194 | 4 | 0 | 1 | 194 | 14 | 207 | 3.00E-106 | 394.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59442.t1 | G7KGE7 | 97.9 | 194 | 4 | 0 | 1 | 194 | 14 | 207 | 2.2e-106 | 394.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051860 | MS.gene59442 | 0.801579 | 8.42E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene59442 | 0.821057 | 4.94E-53 | -1.69E-46 |
| MS.gene053074 | MS.gene59442 | 0.805034 | 1.62E-49 | -1.69E-46 |
| MS.gene053184 | MS.gene59442 | 0.803288 | 3.74E-49 | -1.69E-46 |
| MS.gene054185 | MS.gene59442 | 0.802487 | 5.48E-49 | -1.69E-46 |
| MS.gene054713 | MS.gene59442 | 0.903959 | 2.02E-79 | -1.69E-46 |
| MS.gene055608 | MS.gene59442 | 0.830761 | 2.45E-55 | -1.69E-46 |
| MS.gene055860 | MS.gene59442 | 0.834744 | 2.51E-56 | -1.69E-46 |
| MS.gene058042 | MS.gene59442 | 0.877798 | 4.71E-69 | -1.69E-46 |
| MS.gene058168 | MS.gene59442 | 0.811522 | 6.71E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59442.t1 | MTR_5g021610 | 97.938 | 194 | 4 | 0 | 1 | 194 | 14 | 207 | 1.16e-144 | 400 |
| MS.gene59442.t1 | MTR_8g076160 | 75.897 | 195 | 44 | 3 | 1 | 194 | 1 | 193 | 7.99e-108 | 307 |
| MS.gene59442.t1 | MTR_2g005880 | 53.846 | 182 | 80 | 1 | 12 | 193 | 3 | 180 | 1.75e-66 | 202 |
| MS.gene59442.t1 | MTR_4g114080 | 58.571 | 140 | 54 | 1 | 12 | 151 | 5 | 140 | 1.03e-56 | 177 |
| MS.gene59442.t1 | MTR_4g011020 | 55.479 | 146 | 57 | 2 | 12 | 155 | 9 | 148 | 2.02e-55 | 174 |
| MS.gene59442.t1 | MTR_2g103490 | 54.225 | 142 | 60 | 2 | 12 | 152 | 9 | 146 | 3.54e-51 | 162 |
| MS.gene59442.t1 | MTR_2g103460 | 52.817 | 142 | 62 | 2 | 12 | 152 | 9 | 146 | 3.40e-49 | 157 |
| MS.gene59442.t1 | MTR_0003s0630 | 47.333 | 150 | 77 | 2 | 4 | 152 | 26 | 174 | 6.65e-48 | 155 |
| MS.gene59442.t1 | MTR_1g115170 | 46.617 | 133 | 66 | 2 | 29 | 160 | 3 | 131 | 1.64e-39 | 132 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59442.t1 | AT1G15380 | 58.993 | 139 | 53 | 1 | 12 | 150 | 9 | 143 | 1.42e-58 | 181 |
| MS.gene59442.t1 | AT1G15380 | 58.993 | 139 | 53 | 1 | 12 | 150 | 9 | 143 | 1.42e-58 | 181 |
| MS.gene59442.t1 | AT1G80160 | 57.639 | 144 | 57 | 2 | 12 | 155 | 9 | 148 | 1.78e-58 | 181 |
| MS.gene59442.t1 | AT1G80160 | 56.944 | 144 | 58 | 2 | 12 | 155 | 50 | 189 | 7.17e-58 | 181 |
| MS.gene59442.t1 | AT2G28420 | 52.083 | 144 | 65 | 2 | 13 | 155 | 17 | 157 | 9.32e-53 | 167 |
| MS.gene59442.t1 | AT1G80160 | 53.000 | 100 | 43 | 2 | 56 | 155 | 27 | 122 | 4.10e-33 | 115 |
Find 33 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATCTTCTTTCAAATTTAC+TGG | 0.206523 | 5.4:-13262194 | MS.gene59442:CDS |
| TGGTTATCCTTGGGATTAAT+AGG | 0.256572 | 5.4:+13261972 | None:intergenic |
| CTCTTAAAGCTATGGCTTCT+AGG | 0.268260 | 5.4:+13261425 | None:intergenic |
| GGTAGAAGATGAAGGAATTA+AGG | 0.296959 | 5.4:-13261552 | MS.gene59442:CDS |
| TAAGAAGAGATTAGAAGAAA+AGG | 0.341886 | 5.4:-13261597 | MS.gene59442:CDS |
| GAAGCAGTGCATGAAGAAAT+AGG | 0.352827 | 5.4:+13261455 | None:intergenic |
| GAATCCTCCACACTTGGAAT+TGG | 0.363613 | 5.4:+13261394 | None:intergenic |
| CATGAAGAAATAGGAATGAT+TGG | 0.374847 | 5.4:+13261464 | None:intergenic |
| AAGAAGAGATTAGAAGAAAA+GGG | 0.385631 | 5.4:-13261596 | MS.gene59442:CDS |
| GAAAGAAGATGGTCGTTTGA+TGG | 0.396618 | 5.4:+13262205 | None:intergenic |
| AGCTCAATCATGTAGCCATC+AGG | 0.416541 | 5.4:+13261506 | None:intergenic |
| GAATGAGATATGGTTATCCT+TGG | 0.430947 | 5.4:+13261962 | None:intergenic |
| GGATAACCATATCTCATTCC+AGG | 0.432811 | 5.4:-13261958 | MS.gene59442:intron |
| GTATCAAATTCATCAATGTC+TGG | 0.469039 | 5.4:+13262014 | None:intergenic |
| AAAGAAGATGGTCGTTTGAT+GGG | 0.476824 | 5.4:+13262206 | None:intergenic |
| CCAGTAAATTTGAAAGAAGA+TGG | 0.495096 | 5.4:+13262194 | None:intergenic |
| ATGAAGAAATAGGAATGATT+GGG | 0.499317 | 5.4:+13261465 | None:intergenic |
| CTGCATGAATCCTCCACACT+TGG | 0.500461 | 5.4:+13261388 | None:intergenic |
| AATGAGATATGGTTATCCTT+GGG | 0.506176 | 5.4:+13261963 | None:intergenic |
| CTTTCAAATTTACTGGAGCA+TGG | 0.508696 | 5.4:-13262187 | MS.gene59442:intron |
| AAGTAGATCTCTTAAAGCTA+TGG | 0.522280 | 5.4:+13261417 | None:intergenic |
| ATTCATGCAGAATGTTATGA+TGG | 0.532345 | 5.4:-13261375 | MS.gene59442:CDS |
| TCATGTAGCCATCAGGGTCA+TGG | 0.545015 | 5.4:+13261513 | None:intergenic |
| AATGAGATATGTTACAGCAT+TGG | 0.548154 | 5.4:-13261573 | MS.gene59442:CDS |
| ACAGCATTGGTAGAAGATGA+AGG | 0.580373 | 5.4:-13261560 | MS.gene59442:CDS |
| GTGTTCTTCCATGACCCTGA+TGG | 0.589307 | 5.4:-13261521 | MS.gene59442:CDS |
| ACATGATTCAATGAGAGCAA+TGG | 0.593307 | 5.4:+13262293 | None:intergenic |
| AACGAGGCCTATTAATCCCA+AGG | 0.600112 | 5.4:-13261979 | MS.gene59442:CDS |
| GCTCAATCATGTAGCCATCA+GGG | 0.602960 | 5.4:+13261507 | None:intergenic |
| TCTACTTCCAATTCCAAGTG+TGG | 0.614729 | 5.4:-13261401 | MS.gene59442:CDS |
| ACTTCCAATTCCAAGTGTGG+AGG | 0.621587 | 5.4:-13261398 | MS.gene59442:CDS |
| TATGATGGAAAGCTTGAGCA+TGG | 0.636263 | 5.4:-13261360 | MS.gene59442:CDS |
| ATACTTACATGAATGAAACG+AGG | 0.744566 | 5.4:-13261995 | MS.gene59442:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGCTTATTTTTTTATATTTT+TGG | - | chr5.4:13261504-13261523 | MS.gene59442:CDS | 10.0% |
| !! | CCAAAAATTTATATACAATA+TGG | - | chr5.4:13262016-13262035 | MS.gene59442:CDS | 15.0% |
| !!! | AATATAGTCATTTTTTGAAA+TGG | - | chr5.4:13261758-13261777 | MS.gene59442:intron | 15.0% |
| !!! | CCATATTGTATATAAATTTT+TGG | + | chr5.4:13262019-13262038 | None:intergenic | 15.0% |
| !!! | TATAAATCTTTATTTTTACC+TGG | + | chr5.4:13261729-13261748 | None:intergenic | 15.0% |
| !! | GCACATAATAATTATAACAA+AGG | + | chr5.4:13261920-13261939 | None:intergenic | 20.0% |
| !! | TGTTATAATTATTATGTGCA+TGG | - | chr5.4:13261921-13261940 | MS.gene59442:intron | 20.0% |
| !!! | AGGTTTTATAATTATGGTAT+TGG | - | chr5.4:13261610-13261629 | MS.gene59442:CDS | 20.0% |
| !!! | TTTTGCAGGTTTTATAATTA+TGG | - | chr5.4:13261604-13261623 | MS.gene59442:CDS | 20.0% |
| ! | AAGAAGAGATTAGAAGAAAA+GGG | - | chr5.4:13262070-13262089 | MS.gene59442:intron | 25.0% |
| ! | ATGAAGAAATAGGAATGATT+GGG | + | chr5.4:13262204-13262223 | None:intergenic | 25.0% |
| ! | TAAGAAGAGATTAGAAGAAA+AGG | - | chr5.4:13262069-13262088 | MS.gene59442:intron | 25.0% |
| ! | TAAGTAAATAATTAACCGCA+TGG | - | chr5.4:13261953-13261972 | MS.gene59442:intron | 25.0% |
| !! | CAATTTTATGAGGATGTTTT+GGG | - | chr5.4:13261430-13261449 | MS.gene59442:CDS | 25.0% |
| !!! | GCTTTTAACTTTTTTTTTGC+AGG | - | chr5.4:13261590-13261609 | MS.gene59442:CDS | 25.0% |
| !!! | GTCATTTTTTGAAATGGTTA+TGG | - | chr5.4:13261764-13261783 | MS.gene59442:intron | 25.0% |
| AAGTAGATCTCTTAAAGCTA+TGG | + | chr5.4:13262252-13262271 | None:intergenic | 30.0% | |
| AATGAGATATGGTTATCCTT+GGG | + | chr5.4:13261706-13261725 | None:intergenic | 30.0% | |
| AATGAGATATGTTACAGCAT+TGG | - | chr5.4:13262093-13262112 | MS.gene59442:intron | 30.0% | |
| ATACTTACATGAATGAAACG+AGG | - | chr5.4:13261671-13261690 | MS.gene59442:intron | 30.0% | |
| ATGGAAATCTATGCATATGT+AGG | - | chr5.4:13261818-13261837 | MS.gene59442:intron | 30.0% | |
| ATTCATGCAGAATGTTATGA+TGG | - | chr5.4:13262291-13262310 | MS.gene59442:CDS | 30.0% | |
| CATGAAGAAATAGGAATGAT+TGG | + | chr5.4:13262205-13262224 | None:intergenic | 30.0% | |
| CCAGTAAATTTGAAAGAAGA+TGG | + | chr5.4:13261475-13261494 | None:intergenic | 30.0% | |
| CCATCTTCTTTCAAATTTAC+TGG | - | chr5.4:13261472-13261491 | MS.gene59442:CDS | 30.0% | |
| GTATCAAATTCATCAATGTC+TGG | + | chr5.4:13261655-13261674 | None:intergenic | 30.0% | |
| TGGAAATCTATGCATATGTA+GGG | - | chr5.4:13261819-13261838 | MS.gene59442:intron | 30.0% | |
| ! | GCAATTTTATGAGGATGTTT+TGG | - | chr5.4:13261429-13261448 | MS.gene59442:CDS | 30.0% |
| ! | GGAATCTATGCAATTTTATG+AGG | - | chr5.4:13261420-13261439 | MS.gene59442:CDS | 30.0% |
| ! | TTTTTACCTGGAATGAGATA+TGG | + | chr5.4:13261717-13261736 | None:intergenic | 30.0% |
| !!! | ATATGGTTTTTTGCAGTGTA+CGG | - | chr5.4:13262033-13262052 | MS.gene59442:CDS | 30.0% |
| AAAGAAGATGGTCGTTTGAT+GGG | + | chr5.4:13261463-13261482 | None:intergenic | 35.0% | |
| ACATGATTCAATGAGAGCAA+TGG | + | chr5.4:13261376-13261395 | None:intergenic | 35.0% | |
| AGCTAAATTTGTGATCCATG+CGG | + | chr5.4:13261971-13261990 | None:intergenic | 35.0% | |
| CTTTCAAATTTACTGGAGCA+TGG | - | chr5.4:13261479-13261498 | MS.gene59442:CDS | 35.0% | |
| GAATGAGATATGGTTATCCT+TGG | + | chr5.4:13261707-13261726 | None:intergenic | 35.0% | |
| GGTAGAAGATGAAGGAATTA+AGG | - | chr5.4:13262114-13262133 | MS.gene59442:intron | 35.0% | |
| TGGTTATCCTTGGGATTAAT+AGG | + | chr5.4:13261697-13261716 | None:intergenic | 35.0% | |
| ! | TCTTTTGTGTAGATCAGTGT+TGG | - | chr5.4:13261399-13261418 | MS.gene59442:CDS | 35.0% |
| CTCTTAAAGCTATGGCTTCT+AGG | + | chr5.4:13262244-13262263 | None:intergenic | 40.0% | |
| GAAAGAAGATGGTCGTTTGA+TGG | + | chr5.4:13261464-13261483 | None:intergenic | 40.0% | |
| GAAGCAGTGCATGAAGAAAT+AGG | + | chr5.4:13262214-13262233 | None:intergenic | 40.0% | |
| GGATAACCATATCTCATTCC+AGG | - | chr5.4:13261708-13261727 | MS.gene59442:intron | 40.0% | |
| TCTACTTCCAATTCCAAGTG+TGG | - | chr5.4:13262265-13262284 | MS.gene59442:CDS | 40.0% | |
| ! | TATGATGGAAAGCTTGAGCA+TGG | - | chr5.4:13262306-13262325 | MS.gene59442:CDS | 40.0% |
| !! | ACAGCATTGGTAGAAGATGA+AGG | - | chr5.4:13262106-13262125 | MS.gene59442:intron | 40.0% |
| AACGAGGCCTATTAATCCCA+AGG | - | chr5.4:13261687-13261706 | MS.gene59442:intron | 45.0% | |
| ACTTCCAATTCCAAGTGTGG+AGG | - | chr5.4:13262268-13262287 | MS.gene59442:CDS | 45.0% | |
| AGCTCAATCATGTAGCCATC+AGG | + | chr5.4:13262163-13262182 | None:intergenic | 45.0% | |
| GAATCCTCCACACTTGGAAT+TGG | + | chr5.4:13262275-13262294 | None:intergenic | 45.0% | |
| GCTCAATCATGTAGCCATCA+GGG | + | chr5.4:13262162-13262181 | None:intergenic | 45.0% | |
| !! | ATAAATTTATATACTAAAAA+TGG | - | chr5.4:13261799-13261818 | MS.gene59442:intron | 5.0% |
| CTGCATGAATCCTCCACACT+TGG | + | chr5.4:13262281-13262300 | None:intergenic | 50.0% | |
| GTGTTCTTCCATGACCCTGA+TGG | - | chr5.4:13262145-13262164 | MS.gene59442:intron | 50.0% | |
| TCATGTAGCCATCAGGGTCA+TGG | + | chr5.4:13262156-13262175 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 13261337 | 13262351 | 13261337 | ID=MS.gene59442 |
| chr5.4 | mRNA | 13261337 | 13262351 | 13261337 | ID=MS.gene59442.t1;Parent=MS.gene59442 |
| chr5.4 | exon | 13262188 | 13262351 | 13262188 | ID=MS.gene59442.t1.exon1;Parent=MS.gene59442.t1 |
| chr5.4 | CDS | 13262188 | 13262351 | 13262188 | ID=cds.MS.gene59442.t1;Parent=MS.gene59442.t1 |
| chr5.4 | exon | 13261959 | 13262076 | 13261959 | ID=MS.gene59442.t1.exon2;Parent=MS.gene59442.t1 |
| chr5.4 | CDS | 13261959 | 13262076 | 13261959 | ID=cds.MS.gene59442.t1;Parent=MS.gene59442.t1 |
| chr5.4 | exon | 13261337 | 13261639 | 13261337 | ID=MS.gene59442.t1.exon3;Parent=MS.gene59442.t1 |
| chr5.4 | CDS | 13261337 | 13261639 | 13261337 | ID=cds.MS.gene59442.t1;Parent=MS.gene59442.t1 |
| Gene Sequence |
| Protein sequence |
