AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053184


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053184.t1 MTR_4g007550 81.203 133 16 3 1 128 1 129 1.42e-62 187
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 16 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTAAGATGTAGGAAACTA+TGG 0.353441 4.2:+92236623 MS.gene053184:intron
GCTCTACATCCAAGTTATCT+TGG 0.366777 4.2:+92236801 MS.gene053184:CDS
ATTAAGAAAGCTAGAGGAAA+AGG 0.431070 4.2:+92236728 MS.gene053184:CDS
GCTAGAGGAAAAGGAGCTAA+AGG 0.517100 4.2:+92236737 MS.gene053184:CDS
GAAGAATGCTTACTACTAGT+AGG 0.521612 4.2:-92236515 None:intergenic
AGGTGAAATCCAAGATAACT+TGG 0.531170 4.2:-92236810 None:intergenic
AACTATGGAAGCATAAAAGA+AGG 0.547768 4.2:+92236638 MS.gene053184:CDS
AGAAGATAAGAAACATATCA+AGG 0.562331 4.2:+92236676 MS.gene053184:CDS
TCATAGCAACTTAACATGAA+AGG 0.582658 4.2:-92236867 None:intergenic
GACTCGATTAAGAAAGCTAG+AGG 0.596569 4.2:+92236722 MS.gene053184:CDS
AAACTCGTCAAATAGCATCA+TGG 0.602558 4.2:+92236481 MS.gene053184:CDS
AAAACAAAGCTTACACACAC+AGG 0.629592 4.2:-92236830 None:intergenic
AAAGAAGGAAGCAAGTTGCA+CGG 0.651204 4.2:+92236653 MS.gene053184:CDS
AGAGGAAAAGGAGCTAAAGG+TGG 0.683202 4.2:+92236740 MS.gene053184:CDS
AGATAAGAAACATATCAAGG+AGG 0.727302 4.2:+92236679 MS.gene053184:CDS
GTAGAGCTTCGAGAACGACG+AGG 0.744495 4.2:-92236785 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAAGATAAGAAACATATCA+AGG + chr4.2:92236676-92236695 MS.gene053184:CDS 25.0%
! TATAGCATGAGTACAATTTA+AGG - chr4.2:92236561-92236580 None:intergenic 25.0%
! TATTAAGATGTAGGAAACTA+TGG + chr4.2:92236623-92236642 MS.gene053184:intron 25.0%
! TGCTTATTCTATTAAGATGT+AGG + chr4.2:92236614-92236633 MS.gene053184:intron 25.0%
AGATAAGAAACATATCAAGG+AGG + chr4.2:92236679-92236698 MS.gene053184:CDS 30.0%
ATTAAGAAAGCTAGAGGAAA+AGG + chr4.2:92236728-92236747 MS.gene053184:CDS 30.0%
TCATAGCAACTTAACATGAA+AGG - chr4.2:92236870-92236889 None:intergenic 30.0%
! AACTATGGAAGCATAAAAGA+AGG + chr4.2:92236638-92236657 MS.gene053184:CDS 30.0%
!!! ACTCAAGCTATATCGATTTT+AGG + chr4.2:92236434-92236453 MS.gene053184:CDS 30.0%
AAAACAAAGCTTACACACAC+AGG - chr4.2:92236833-92236852 None:intergenic 35.0%
AAACTCGTCAAATAGCATCA+TGG + chr4.2:92236481-92236500 MS.gene053184:CDS 35.0%
AGGTGAAATCCAAGATAACT+TGG - chr4.2:92236813-92236832 None:intergenic 35.0%
GAAGAATGCTTACTACTAGT+AGG - chr4.2:92236518-92236537 None:intergenic 35.0%
AAAGAAGGAAGCAAGTTGCA+CGG + chr4.2:92236653-92236672 MS.gene053184:CDS 40.0%
GACTCGATTAAGAAAGCTAG+AGG + chr4.2:92236722-92236741 MS.gene053184:CDS 40.0%
GCTCTACATCCAAGTTATCT+TGG + chr4.2:92236801-92236820 MS.gene053184:CDS 40.0%
AAGGAAACGCTCCTTCAGAA+AGG - chr4.2:92236542-92236561 None:intergenic 45.0%
AGAGGAAAAGGAGCTAAAGG+TGG + chr4.2:92236740-92236759 MS.gene053184:CDS 45.0%
AGCATTCTTCGCCTTTCTGA+AGG + chr4.2:92236528-92236547 MS.gene053184:intron 45.0%
GCTAGAGGAAAAGGAGCTAA+AGG + chr4.2:92236737-92236756 MS.gene053184:CDS 45.0%
GTAGAGCTTCGAGAACGACG+AGG - chr4.2:92236788-92236807 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 92236392 92236889 92236392 ID=MS.gene053184
chr4.2 mRNA 92236392 92236889 92236392 ID=MS.gene053184.t1;Parent=MS.gene053184
chr4.2 exon 92236392 92236524 92236392 ID=MS.gene053184.t1.exon1;Parent=MS.gene053184.t1
chr4.2 CDS 92236392 92236524 92236392 ID=cds.MS.gene053184.t1;Parent=MS.gene053184.t1
chr4.2 exon 92236636 92236889 92236636 ID=MS.gene053184.t1.exon2;Parent=MS.gene053184.t1
chr4.2 CDS 92236636 92236889 92236636 ID=cds.MS.gene053184.t1;Parent=MS.gene053184.t1
Gene Sequence

>MS.gene053184

ATGAGAGTAAGATTTCTTACATGTTTCATCGCTGTTTTATGCACTCAAGCTATATCGATTTTAGGTTTCAGTTCAGAACATAGAGCTTTAAACTCGTCAAATAGCATCATGGAAACAATGCAACCTACTAGTAGTAAGCATTCTTCGCCTTTCTGAAGGAGCGTTTCCTTAAATTGTACTCATGCTATATTTAATTTCTGCTTCTAGATGCTAACTAAATTTTGCTTATTCTATTAAGATGTAGGAAACTATGGAAGCATAAAAGAAGGAAGCAAGTTGCACGGAGAAGATAAGAAACATATCAAGGAGGAAAAGTTTGAACGAGCTTATGACTCGATTAAGAAAGCTAGAGGAAAAGGAGCTAAAGGTGGTGCAAATGATAATCACTCTGAACCTCGTCGTTCTCGAAGCTCTACATCCAAGTTATCTTGGATTTCACCTGTGTGTGTAAGCTTTGTTTTAATATCAGTCTTCTCCTTTCATGTTAAGTTGCTATGA

Protein sequence

>MS.gene053184.t1

MRVRFLTCFIAVLCTQAISILGFSSEHRALNSSNSIMETMQPTSRNYGSIKEGSKLHGEDKKHIKEEKFERAYDSIKKARGKGAKGGANDNHSEPRRSRSSTSKLSWISPVCVSFVLISVFSFHVKLL