AlfalfaGEDB | MS.gene60019

M. sativa cultivar XinJiangDaYe / MS.gene60019

KEGG: -
GO: -
PFAM: PF13639.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60019.t1	XP_003620418.2	100	93	0	0	1	93	268	360	9.80E-47	195.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60019.t1	Q9ZQF9	48.4	95	47	1	1	93	610	704	6.2e-18	91.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60019.t1	G7KMM3	100.0	93	0	0	1	93	268	360	7.0e-47	195.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene60019	0.822888	1.86E-53	-1.69E-46
MS.gene050689	MS.gene60019	0.840651	7.65E-58	-1.69E-46
MS.gene051243	MS.gene60019	0.812542	4.02E-51	-1.69E-46
MS.gene051328	MS.gene60019	0.813712	2.23E-51	-1.69E-46
MS.gene05134	MS.gene60019	0.817776	2.77E-52	-1.69E-46
MS.gene052611	MS.gene60019	0.810141	1.34E-50	-1.69E-46
MS.gene052863	MS.gene60019	0.81348	2.51E-51	-1.69E-46
MS.gene053008	MS.gene60019	0.820359	7.15E-53	-1.69E-46
MS.gene053226	MS.gene60019	0.803269	3.78E-49	-1.69E-46
MS.gene05364	MS.gene60019	0.864038	1.62E-64	-1.69E-46
MS.gene055512	MS.gene60019	0.825821	3.80E-54	-1.69E-46
MS.gene056760	MS.gene60019	0.813934	1.99E-51	-1.69E-46
MS.gene057140	MS.gene60019	0.804079	2.57E-49	-1.69E-46
MS.gene057392	MS.gene60019	0.800246	1.58E-48	-1.69E-46
MS.gene057693	MS.gene60019	0.817025	4.08E-52	-1.69E-46
MS.gene057705	MS.gene60019	0.814114	1.82E-51	-1.69E-46
MS.gene059151	MS.gene60019	0.815603	8.50E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60019.t1	MTR_6g082620	100.000	93	0	0	1	93	268	360	1.44e-64	198
MS.gene60019.t1	MTR_8g106240	72.826	92	25	0	1	92	262	353	1.97e-46	152
MS.gene60019.t1	MTR_1g019200	44.186	86	47	1	1	86	618	702	6.89e-23	91.3
MS.gene60019.t1	MTR_2g078450	41.667	96	49	2	2	90	421	516	1.92e-22	90.1
MS.gene60019.t1	MTR_3g108808	44.211	95	47	2	1	89	438	532	4.56e-21	86.3
MS.gene60019.t1	MTR_1g066430	47.126	87	41	3	1	86	611	693	1.61e-20	84.7
MS.gene60019.t1	MTR_2g078440	45.556	90	45	3	1	90	213	298	2.54e-20	83.2
MS.gene60019.t1	MTR_7g100950	43.956	91	47	2	1	90	460	547	3.43e-20	83.6
MS.gene60019.t1	MTR_7g100950	43.956	91	47	2	1	90	462	549	3.43e-20	83.6
MS.gene60019.t1	MTR_1g009680	43.011	93	47	2	1	87	378	470	6.22e-20	82.8
MS.gene60019.t1	MTR_4g085630	46.512	86	45	1	1	86	467	551	1.05e-19	82.4
MS.gene60019.t1	MTR_3g025940	43.038	79	44	1	9	87	365	442	1.70e-19	81.6
MS.gene60019.t1	MTR_5g016960	46.053	76	40	1	12	87	519	593	7.61e-19	79.7
MS.gene60019.t1	MTR_2g076290	40.404	99	50	3	1	91	233	330	2.72e-18	77.4
MS.gene60019.t1	MTR_1g066400	43.678	87	44	3	1	86	443	525	1.17e-17	76.3
MS.gene60019.t1	MTR_5g016980	43.373	83	43	3	12	92	254	334	3.02e-17	74.7
MS.gene60019.t1	MTR_5g016940	50.000	54	27	0	34	87	288	341	9.77e-17	73.6
MS.gene60019.t1	MTR_3g088100	42.697	89	48	2	1	89	117	202	1.31e-16	71.2
MS.gene60019.t1	MTR_5g079050	41.892	74	42	1	13	86	282	354	1.32e-15	70.5
MS.gene60019.t1	MTR_5g016920	42.857	77	41	2	15	90	71	145	3.68e-15	66.6
MS.gene60019.t1	MTR_5g078900	40.000	75	44	1	13	87	282	355	4.06e-15	68.9
MS.gene60019.t1	MTR_8g037150	34.831	89	57	1	1	89	153	240	4.70e-13	62.4
MS.gene60019.t1	MTR_1g114240	39.362	94	44	2	1	89	247	332	1.86e-12	61.6
MS.gene60019.t1	MTR_1g114240	39.362	94	44	2	1	89	241	326	2.46e-12	61.2
MS.gene60019.t1	MTR_3g072630	43.860	57	32	0	35	91	211	267	5.16e-12	60.1
MS.gene60019.t1	MTR_3g107995	45.833	48	26	0	42	89	314	361	5.61e-12	60.1
MS.gene60019.t1	MTR_3g072630	43.860	57	32	0	35	91	211	267	7.92e-12	59.7
MS.gene60019.t1	MTR_1g011380	45.833	48	26	0	42	89	338	385	1.28e-11	59.3
MS.gene60019.t1	MTR_4g064570	46.667	45	24	0	45	89	181	225	2.56e-11	58.2
MS.gene60019.t1	MTR_1g051105	43.636	55	30	1	36	89	149	203	3.63e-11	57.4
MS.gene60019.t1	MTR_2g007340	45.833	48	26	0	42	89	198	245	3.99e-11	57.8
MS.gene60019.t1	MTR_5g088720	43.750	48	27	0	42	89	197	244	5.23e-11	57.4
MS.gene60019.t1	MTR_5g088720	43.750	48	27	0	42	89	202	249	5.28e-11	57.4
MS.gene60019.t1	MTR_2g097620	37.736	53	33	0	37	89	181	233	5.53e-11	57.4
MS.gene60019.t1	MTR_5g088720	43.750	48	27	0	42	89	243	290	5.56e-11	57.4
MS.gene60019.t1	MTR_4g065670	34.783	92	57	2	1	91	153	242	6.20e-11	56.6
MS.gene60019.t1	MTR_4g065670	34.783	92	57	2	1	91	160	249	7.48e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60019.t1	AT1G17970	54.839	93	38	1	1	93	280	368	7.88e-31	111
MS.gene60019.t1	AT1G73760	54.651	86	36	1	1	86	278	360	6.06e-29	106
MS.gene60019.t1	AT2G15530	48.421	95	47	1	1	93	588	682	1.26e-24	96.3
MS.gene60019.t1	AT2G15530	48.421	95	47	1	1	93	610	704	1.26e-24	96.3
MS.gene60019.t1	AT2G15530	48.421	95	47	1	1	93	610	704	1.26e-24	96.3
MS.gene60019.t1	AT2G15530	48.421	95	47	1	1	93	610	704	1.26e-24	96.3
MS.gene60019.t1	AT2G15530	48.421	95	47	1	1	93	610	704	1.26e-24	96.3
MS.gene60019.t1	AT2G15530	48.421	95	47	1	1	93	610	704	1.26e-24	96.3
MS.gene60019.t1	AT4G31450	47.872	94	41	2	1	86	396	489	1.11e-23	93.6
MS.gene60019.t1	AT4G31450	47.872	94	41	2	1	86	396	489	1.11e-23	93.6
MS.gene60019.t1	AT4G34040	48.837	86	43	1	1	86	576	660	1.12e-23	93.6
MS.gene60019.t1	AT3G15070	40.426	94	53	1	1	91	381	474	1.82e-22	90.1
MS.gene60019.t1	AT3G15070	40.426	94	53	1	1	91	381	474	1.82e-22	90.1
MS.gene60019.t1	AT1G53190	38.776	98	53	1	1	91	391	488	3.45e-22	89.4
MS.gene60019.t1	AT1G53190	38.776	98	53	1	1	91	391	488	3.45e-22	89.4
MS.gene60019.t1	AT5G10650	40.000	90	50	1	1	86	427	516	1.53e-21	87.4
MS.gene60019.t1	AT5G10650	40.000	90	50	1	1	86	427	516	1.53e-21	87.4
MS.gene60019.t1	AT2G15530	48.837	86	42	1	1	84	610	695	2.14e-21	87.0
MS.gene60019.t1	AT5G42940	43.023	86	47	1	1	86	595	678	4.45e-21	86.3
MS.gene60019.t1	AT5G42940	43.023	86	47	1	1	86	595	678	4.45e-21	86.3
MS.gene60019.t1	AT5G24870	41.837	98	51	2	1	93	420	516	9.41e-21	85.1
MS.gene60019.t1	AT5G24870	41.837	98	51	2	1	93	420	516	9.41e-21	85.1
MS.gene60019.t1	AT5G24870	41.837	98	51	2	1	93	420	516	9.41e-21	85.1
MS.gene60019.t1	AT1G45180	48.837	86	42	1	1	86	550	633	1.31e-19	82.0
MS.gene60019.t1	AT1G45180	48.837	86	42	1	1	86	550	633	1.31e-19	82.0
MS.gene60019.t1	AT1G45180	48.837	86	42	1	1	86	550	633	1.31e-19	82.0
MS.gene60019.t1	AT1G45180	48.837	86	42	1	1	86	502	585	1.33e-19	82.0
MS.gene60019.t1	AT5G67120	36.667	90	53	1	1	90	187	272	3.69e-15	68.6
MS.gene60019.t1	AT3G19910	35.955	89	54	1	1	89	247	332	5.71e-14	65.9
MS.gene60019.t1	AT2G37150	43.678	87	45	2	1	86	451	534	1.70e-13	64.7
MS.gene60019.t1	AT2G37150	43.678	87	45	2	1	86	451	534	1.70e-13	64.7
MS.gene60019.t1	AT2G37150	43.678	87	45	2	1	86	451	534	1.70e-13	64.7
MS.gene60019.t1	AT2G37150	43.678	87	45	2	1	86	451	534	1.70e-13	64.7
MS.gene60019.t1	AT2G37150	43.678	87	45	2	1	86	451	534	1.70e-13	64.7
MS.gene60019.t1	AT2G37150	43.678	87	45	2	1	86	451	534	1.70e-13	64.7
MS.gene60019.t1	AT2G37150	43.678	87	45	2	1	86	451	534	1.70e-13	64.7
MS.gene60019.t1	AT2G37150	43.678	87	45	2	1	86	451	534	1.70e-13	64.7
MS.gene60019.t1	AT2G37150	43.678	87	45	2	1	86	451	534	1.70e-13	64.7
MS.gene60019.t1	AT2G37150	43.678	87	45	2	1	86	451	534	1.70e-13	64.7
MS.gene60019.t1	AT2G37150	43.678	87	45	2	1	86	451	534	1.70e-13	64.7
MS.gene60019.t1	AT2G37150	43.678	87	45	2	1	86	504	587	1.75e-13	64.7
MS.gene60019.t1	AT2G37150	43.678	87	45	2	1	86	467	550	1.95e-13	64.3
MS.gene60019.t1	AT2G37150	43.678	87	45	2	1	86	467	550	1.95e-13	64.3
MS.gene60019.t1	AT2G37150	43.678	87	45	2	1	86	467	550	1.95e-13	64.3
MS.gene60019.t1	AT3G63530	34.409	93	60	1	1	93	154	245	4.82e-13	62.4
MS.gene60019.t1	AT3G63530	34.409	93	60	1	1	93	154	245	4.82e-13	62.4
MS.gene60019.t1	AT4G26580	38.596	57	35	0	33	89	277	333	1.40e-12	62.0
MS.gene60019.t1	AT4G26580	38.596	57	35	0	33	89	277	333	1.40e-12	62.0
MS.gene60019.t1	AT2G40830	45.833	48	26	0	42	89	187	234	5.46e-12	60.1
MS.gene60019.t1	AT2G40830	45.833	48	26	0	42	89	187	234	5.46e-12	60.1
MS.gene60019.t1	AT2G40830	45.833	48	26	0	42	89	187	234	5.46e-12	60.1
MS.gene60019.t1	AT2G40830	45.833	48	26	0	42	89	187	234	5.46e-12	60.1
MS.gene60019.t1	AT3G47180	35.484	93	56	3	1	92	121	210	1.67e-11	58.2
MS.gene60019.t1	AT3G56580	43.750	48	27	0	42	89	183	230	2.31e-11	58.5
MS.gene60019.t1	AT3G56580	43.750	48	27	0	42	89	183	230	2.31e-11	58.5
MS.gene60019.t1	AT3G56580	43.750	48	27	0	42	89	183	230	2.31e-11	58.5
MS.gene60019.t1	AT3G10815	34.444	90	50	3	1	89	84	165	5.05e-11	56.6
MS.gene60019.t1	AT5G66160	39.286	56	33	1	35	89	222	277	9.29e-11	56.6
MS.gene60019.t1	AT5G66160	39.286	56	33	1	35	89	222	277	9.87e-11	56.6

Find 11 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTATGAATCAGATGATGAAT+TGG	0.374695	6.1:-72661155	MS.gene60019:CDS
AGAATTGGTTATGTGAAAAC+TGG	0.378566	6.1:-72661399	MS.gene60019:CDS
CTTGAGCTGGGAGAGAGAAT+TGG	0.417630	6.1:-72661414	MS.gene60019:CDS
TTGCAAACTCACTTGACAAA+TGG	0.433202	6.1:+72661284	None:intergenic
TATGAATCAGATGATGAATT+GGG	0.460080	6.1:-72661154	MS.gene60019:CDS
GTAACAGCAATTGCTTGAGC+TGG	0.470112	6.1:-72661427	None:intergenic
CGACTTCTTGCTTACAGACT+GGG	0.478209	6.1:+72661062	None:intergenic
ACGACTTCTTGCTTACAGAC+TGG	0.490694	6.1:+72661061	None:intergenic
TAACAGCAATTGCTTGAGCT+GGG	0.576297	6.1:-72661426	None:intergenic
ACTTTCAGTGTATAAAACAA+TGG	0.605990	6.1:-72661105	MS.gene60019:CDS
AGTCTGTAAGCAAGAAGTCG+TGG	0.680527	6.1:-72661059	MS.gene60019:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAAATATGAAACTTTAATT+TGG	+	chr6.1:72661213-72661232	None:intergenic	10.0%
!	ACTTTCAGTGTATAAAACAA+TGG	-	chr6.1:72661363-72661382	MS.gene60019:CDS	25.0%
!	ATGAGATGAAAAACAACATA+AGG	-	chr6.1:72661103-72661122	MS.gene60019:CDS	25.0%
!	TATGAATCAGATGATGAATT+GGG	-	chr6.1:72661314-72661333	MS.gene60019:CDS	25.0%
	AGAATTGGTTATGTGAAAAC+TGG	-	chr6.1:72661069-72661088	MS.gene60019:CDS	30.0%
	GTATGAATCAGATGATGAAT+TGG	-	chr6.1:72661313-72661332	MS.gene60019:CDS	30.0%
	TGTATCTTTCTTGTGTGTTT+AGG	-	chr6.1:72661286-72661305	MS.gene60019:intron	30.0%
	ATCTTTCTTGTGTGTTTAGG+AGG	-	chr6.1:72661289-72661308	MS.gene60019:intron	35.0%
	TTGCAAACTCACTTGACAAA+TGG	+	chr6.1:72661187-72661206	None:intergenic	35.0%
	ACGACTTCTTGCTTACAGAC+TGG	+	chr6.1:72661410-72661429	None:intergenic	45.0%
	AGTCTGTAAGCAAGAAGTCG+TGG	-	chr6.1:72661409-72661428	MS.gene60019:CDS	45.0%
	CGACTTCTTGCTTACAGACT+GGG	+	chr6.1:72661409-72661428	None:intergenic	45.0%
	CTTGAGCTGGGAGAGAGAAT+TGG	-	chr6.1:72661054-72661073	MS.gene60019:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	72661048	72661442	72661048	ID=MS.gene60019
chr6.1	mRNA	72661048	72661442	72661048	ID=MS.gene60019.t1;Parent=MS.gene60019
chr6.1	exon	72661296	72661442	72661296	ID=MS.gene60019.t1.exon1;Parent=MS.gene60019.t1
chr6.1	CDS	72661296	72661442	72661296	ID=cds.MS.gene60019.t1;Parent=MS.gene60019.t1
chr6.1	exon	72661048	72661182	72661048	ID=MS.gene60019.t1.exon2;Parent=MS.gene60019.t1
chr6.1	CDS	72661048	72661182	72661048	ID=cds.MS.gene60019.t1;Parent=MS.gene60019.t1

Gene Sequence

>MS.gene60019

CAATTGCTTGAGCTGGGAGAGAGAATTGGTTATGTGAAAACTGGACTTAAAGAAGATGAGATGAAAAACAACATAAGGAAAATTAAGCTTTTGATTTCAAATGATTCATCAAAGCATCAAATAGACAAAAAGTGCACCATTTGTCAAGTGAGTTTGCAAAATCCAAATTAAAGTTTCATATTTAAAAAGTTCATATCATCACTTTATATCATTCATTTGCTATATAGTATAATGTTTATGTATCTTTCTTGTGTGTTTAGGAGGAGTATGAATCAGATGATGAATTGGGTAGATTACACTGTGAACACAGCTATCACTTTCAGTGTATAAAACAATGGCTTGTGCTTAAGAACTTCTGCCCAGTCTGTAAGCAAGAAGTCGTGGTTCGACCTTGA

Protein sequence

>MS.gene60019.t1

QLLELGERIGYVKTGLKEDEMKNNIRKIKLLISNDSSKHQIDKKCTICQEEYESDDELGRLHCEHSYHFQCIKQWLVLKNFCPVCKQEVVVRP