AlfalfaGEDB | MS.gene60577

M. sativa cultivar XinJiangDaYe / MS.gene60577

KEGG: -
GO: GO:0003674 GO:0005488 GO:0030246
PFAM: PF00139.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60577.t1	GAU17896.1	73	141	36	1	1	141	107	245	1.50E-46	195.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60577.t1	Q41160	63.9	133	45	2	3	135	111	240	4.1e-40	165.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60577.t1	A0A072VPS3	71.4	140	38	1	2	141	113	250	2.7e-45	191.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04932	MS.gene60577	0.82702	1.97E-54	-1.69E-46
MS.gene049549	MS.gene60577	0.824492	7.84E-54	-1.69E-46
MS.gene052211	MS.gene60577	0.808112	3.63E-50	-1.69E-46
MS.gene05240	MS.gene60577	0.802345	5.86E-49	-1.69E-46
MS.gene052706	MS.gene60577	0.802654	5.06E-49	-1.69E-46
MS.gene054146	MS.gene60577	0.801988	6.94E-49	-1.69E-46
MS.gene054277	MS.gene60577	0.81099	8.76E-51	-1.69E-46
MS.gene054742	MS.gene60577	0.80495	1.69E-49	-1.69E-46
MS.gene056330	MS.gene60577	0.807335	5.32E-50	-1.69E-46
MS.gene056590	MS.gene60577	0.80542	1.35E-49	-1.69E-46
MS.gene056591	MS.gene60577	0.80467	1.93E-49	-1.69E-46
MS.gene056690	MS.gene60577	0.801939	7.11E-49	-1.69E-46
MS.gene056697	MS.gene60577	0.809051	2.29E-50	-1.69E-46
MS.gene056920	MS.gene60577	0.81831	2.10E-52	-1.69E-46
MS.gene056922	MS.gene60577	0.806518	7.91E-50	-1.69E-46
MS.gene057261	MS.gene60577	0.811177	7.97E-51	-1.69E-46
MS.gene058381	MS.gene60577	0.829987	3.79E-55	-1.69E-46
MS.gene059955	MS.gene60577	0.801033	1.09E-48	-1.69E-46
MS.gene060087	MS.gene60577	0.841614	4.27E-58	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60577.t1	MTR_1g090973	71.631	141	38	1	1	141	112	250	3.82e-63	194
MS.gene60577.t1	MTR_1g090960	61.069	131	28	1	3	133	99	206	4.84e-46	150
MS.gene60577.t1	MTR_0163s0020	52.448	143	66	2	1	143	112	252	1.16e-42	142
MS.gene60577.t1	MTR_0684s0020	52.448	143	66	2	1	143	111	251	1.67e-42	142
MS.gene60577.t1	MTR_3g054170	53.623	138	62	2	4	141	109	244	4.41e-41	137
MS.gene60577.t1	MTR_1g090957	56.391	133	56	2	3	135	110	240	1.33e-40	137
MS.gene60577.t1	MTR_3g027280	56.391	133	56	2	3	135	122	252	1.93e-40	137
MS.gene60577.t1	MTR_3g047140	54.015	137	60	3	3	138	114	248	1.53e-39	134
MS.gene60577.t1	MTR_3g054180	52.518	139	64	2	4	141	113	250	1.17e-38	131
MS.gene60577.t1	MTR_1g090943	52.713	129	60	1	4	132	116	243	1.53e-36	126
MS.gene60577.t1	MTR_3g054200	51.471	136	64	2	3	138	108	241	2.20e-35	123
MS.gene60577.t1	MTR_1g090937	46.970	132	68	2	1	132	113	242	1.85e-34	121
MS.gene60577.t1	MTR_1g090930	48.889	135	65	3	6	138	120	252	5.12e-31	112
MS.gene60577.t1	MTR_8g068040	41.892	148	65	6	6	139	106	246	1.34e-24	95.5
MS.gene60577.t1	MTR_8g067980	42.963	135	68	3	6	132	108	241	3.10e-24	94.4
MS.gene60577.t1	MTR_8g068030	40.000	145	70	5	6	134	106	249	1.36e-22	90.1
MS.gene60577.t1	MTR_8g068050	43.885	139	66	6	6	134	121	257	3.30e-22	91.7
MS.gene60577.t1	MTR_4g023810	39.850	133	74	4	4	134	110	238	1.84e-20	84.7
MS.gene60577.t1	MTR_8g067990	38.519	135	74	4	6	132	108	241	2.21e-20	84.3
MS.gene60577.t1	MTR_5g031100	40.288	139	69	7	6	132	124	260	5.04e-20	83.6
MS.gene60577.t1	MTR_5g031120	41.429	140	67	7	6	132	124	261	9.83e-20	82.8
MS.gene60577.t1	MTR_5g031140	39.286	140	70	7	6	132	131	268	5.68e-18	78.2
MS.gene60577.t1	MTR_5g031160	39.860	143	65	8	6	132	132	269	7.50e-18	77.8
MS.gene60577.t1	MTR_3g463370	37.500	136	75	3	6	132	70	204	1.37e-16	73.9
MS.gene60577.t1	MTR_7g115740	37.143	140	72	5	6	132	106	242	2.32e-15	72.0
MS.gene60577.t1	MTR_8g023510	40.299	134	72	6	4	132	111	241	2.61e-15	70.9
MS.gene60577.t1	MTR_4g093110	36.879	141	69	8	6	132	112	246	1.62e-14	69.7
MS.gene60577.t1	MTR_8g067735	37.226	137	76	4	6	133	126	261	2.00e-14	69.3
MS.gene60577.t1	MTR_4g093140	34.043	141	77	7	4	132	110	246	4.88e-14	68.6
MS.gene60577.t1	MTR_5g031030	34.043	141	82	4	6	136	121	260	1.49e-13	66.2
MS.gene60577.t1	MTR_8g067630	36.620	142	78	5	6	137	111	250	2.96e-13	66.2
MS.gene60577.t1	MTR_8g067960	36.735	147	83	5	1	139	86	230	3.66e-13	64.7
MS.gene60577.t1	MTR_7g116130	35.766	137	77	4	6	132	108	243	9.41e-12	61.6
MS.gene60577.t1	MTR_8g067690	37.589	141	77	5	2	132	137	276	2.43e-11	60.5
MS.gene60577.t1	MTR_4g056320	45.000	80	39	2	54	131	138	214	3.84e-11	59.3
MS.gene60577.t1	MTR_8g067940	36.806	144	72	9	6	132	122	263	4.30e-11	59.3
MS.gene60577.t1	MTR_4g093070	29.452	146	90	5	4	138	110	253	4.76e-11	59.7
MS.gene60577.t1	MTR_0015s0030	33.099	142	78	5	5	133	101	238	8.73e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60577.t1	AT5G65600	40.714	140	67	6	6	131	117	254	8.17e-17	76.3
MS.gene60577.t1	AT5G10530	41.727	139	67	6	6	132	100	236	8.45e-17	76.3

Find 39 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAAACCTTGGAGTTGTTAA+TGG	0.284776	1.2:-69146491	MS.gene60577:CDS
AATTCCAACATGTGAGTGTT+TGG	0.311826	1.2:+69146403	None:intergenic
AACAGAGTTGGCAGGAATAT+TGG	0.331936	1.2:+69146517	None:intergenic
TAAAGACAGTGAAATGGAAT+AGG	0.341311	1.2:-69146358	MS.gene60577:CDS
GTCCTCACAATCCATTTGTT+GGG	0.372402	1.2:-69146463	MS.gene60577:CDS
ATTCCAACATGTGAGTGTTT+GGG	0.396108	1.2:+69146404	None:intergenic
ATACTAGTTCCACTGGTTCA+TGG	0.408344	1.2:-69146430	MS.gene60577:CDS
TTTCTTTAAAGACAGTGAAA+TGG	0.423423	1.2:-69146364	MS.gene60577:CDS
AAACCAATCCTTACGGTGTT+CGG	0.435951	1.2:+69146206	None:intergenic
ATAGTTGAAAAGGTACCATC+TGG	0.449708	1.2:+69146260	None:intergenic
TCAAACTCTACCCCAACAAA+TGG	0.455076	1.2:+69146452	None:intergenic
CTTCCGAACACCGTAAGGAT+TGG	0.469222	1.2:-69146209	MS.gene60577:CDS
AGTCCTCACAATCCATTTGT+TGG	0.471870	1.2:-69146464	MS.gene60577:CDS
AAAGACAGTGAAATGGAATA+GGG	0.472851	1.2:-69146357	MS.gene60577:CDS
AACTCTGTTGGTGGAAACCT+TGG	0.476055	1.2:-69146503	MS.gene60577:CDS
GCAGGAATATTGGCTCGATC+TGG	0.482568	1.2:+69146527	None:intergenic
GAGTTTGATACTAGTTCCAC+TGG	0.484484	1.2:-69146437	MS.gene60577:CDS
TACTAGTTCCACTGGTTCAT+GGG	0.490856	1.2:-69146429	MS.gene60577:CDS
GACTTGAGCAATAGTTGAAA+AGG	0.492838	1.2:+69146250	None:intergenic
TGTCTTAGAGAGATTGTCAT+AGG	0.497716	1.2:+69146304	None:intergenic
AAAAGAGTTGTTTAGCTGAC+CGG	0.521308	1.2:+69146160	None:intergenic
ATTCCTGCCAACTCTGTTGG+TGG	0.528629	1.2:-69146512	MS.gene60577:CDS
AATATTCCTGCCAACTCTGT+TGG	0.548051	1.2:-69146515	MS.gene60577:CDS
AAGGTTTCCACCAACAGAGT+TGG	0.550040	1.2:+69146505	None:intergenic
TTTCCACCAACAGAGTTGGC+AGG	0.550472	1.2:+69146509	None:intergenic
ATGAAGACAAGTCCACTAGC+AGG	0.558627	1.2:+69146560	None:intergenic
CAGAGCTTCCGAACACCGTA+AGG	0.561999	1.2:-69146214	MS.gene60577:CDS
GCTGCCACATCAAGAGGTGC+CGG	0.566269	1.2:-69146179	MS.gene60577:CDS
GGAATATTGGCTCGATCTGG+TGG	0.573690	1.2:+69146530	None:intergenic
GTTGTTGTATATTACCCAGA+TGG	0.588747	1.2:-69146275	MS.gene60577:CDS
TCCTCACAATCCATTTGTTG+GGG	0.590344	1.2:-69146462	MS.gene60577:CDS
ACCCCAACAAATGGATTGTG+AGG	0.592855	1.2:+69146461	None:intergenic
GATCCCAAACACTCACATGT+TGG	0.594093	1.2:-69146407	MS.gene60577:CDS
TGTTTCCATTAACAACTCCA+AGG	0.599188	1.2:+69146486	None:intergenic
GAATAGGGTGAGTGGCGCCT+TGG	0.611314	1.2:-69146342	MS.gene60577:CDS
CTGACCGGCACCTCTTGATG+TGG	0.630323	1.2:+69146175	None:intergenic
GTGAAATGGAATAGGGTGAG+TGG	0.643420	1.2:-69146350	MS.gene60577:CDS
TAGTTGAAAAGGTACCATCT+GGG	0.650948	1.2:+69146261	None:intergenic
TTTGGGATCCCATGAACCAG+TGG	0.654947	1.2:+69146421	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTCTTTAAAGACAGTGAAA+TGG	-	chr1.2:69146353-69146372	MS.gene60577:CDS	25.0%
	AAAGACAGTGAAATGGAATA+GGG	-	chr1.2:69146360-69146379	MS.gene60577:CDS	30.0%
	TAAAGACAGTGAAATGGAAT+AGG	-	chr1.2:69146359-69146378	MS.gene60577:CDS	30.0%
!	CAGCTAAACAACTCTTTTAT+GGG	-	chr1.2:69146562-69146581	MS.gene60577:CDS	30.0%
!	TCAGCTAAACAACTCTTTTA+TGG	-	chr1.2:69146561-69146580	MS.gene60577:CDS	30.0%
	AAAAGAGTTGTTTAGCTGAC+CGG	+	chr1.2:69146560-69146579	None:intergenic	35.0%
	AATTCCAACATGTGAGTGTT+TGG	+	chr1.2:69146317-69146336	None:intergenic	35.0%
	AGCAGAAAAACCAATCCTTA+CGG	+	chr1.2:69146521-69146540	None:intergenic	35.0%
	ATTCCAACATGTGAGTGTTT+GGG	+	chr1.2:69146316-69146335	None:intergenic	35.0%
	GACTTGAGCAATAGTTGAAA+AGG	+	chr1.2:69146470-69146489	None:intergenic	35.0%
	GTTGTTGTATATTACCCAGA+TGG	-	chr1.2:69146442-69146461	MS.gene60577:CDS	35.0%
	TGTCTTAGAGAGATTGTCAT+AGG	+	chr1.2:69146416-69146435	None:intergenic	35.0%
	TGTTTCCATTAACAACTCCA+AGG	+	chr1.2:69146234-69146253	None:intergenic	35.0%
!	GGAGATTTTTACATTCACCA+AGG	+	chr1.2:69146395-69146414	None:intergenic	35.0%
!!	ATAGTTGAAAAGGTACCATC+TGG	+	chr1.2:69146460-69146479	None:intergenic	35.0%
!!	TAGTTGAAAAGGTACCATCT+GGG	+	chr1.2:69146459-69146478	None:intergenic	35.0%
	AAACCAATCCTTACGGTGTT+CGG	+	chr1.2:69146514-69146533	None:intergenic	40.0%
	AACAGAGTTGGCAGGAATAT+TGG	+	chr1.2:69146203-69146222	None:intergenic	40.0%
	AATATTCCTGCCAACTCTGT+TGG	-	chr1.2:69146202-69146221	MS.gene60577:CDS	40.0%
	AGTCCTCACAATCCATTTGT+TGG	-	chr1.2:69146253-69146272	MS.gene60577:CDS	40.0%
	GAGTTTGATACTAGTTCCAC+TGG	-	chr1.2:69146280-69146299	MS.gene60577:CDS	40.0%
	GGAAACCTTGGAGTTGTTAA+TGG	-	chr1.2:69146226-69146245	MS.gene60577:CDS	40.0%
	GTCCTCACAATCCATTTGTT+GGG	-	chr1.2:69146254-69146273	MS.gene60577:CDS	40.0%
	TCAAACTCTACCCCAACAAA+TGG	+	chr1.2:69146268-69146287	None:intergenic	40.0%
!	TCCTCACAATCCATTTGTTG+GGG	-	chr1.2:69146255-69146274	MS.gene60577:CDS	40.0%
!!	ATACTAGTTCCACTGGTTCA+TGG	-	chr1.2:69146287-69146306	MS.gene60577:CDS	40.0%
!!	TACTAGTTCCACTGGTTCAT+GGG	-	chr1.2:69146288-69146307	MS.gene60577:CDS	40.0%
	AAGGTTTCCACCAACAGAGT+TGG	+	chr1.2:69146215-69146234	None:intergenic	45.0%
	ATGAAGACAAGTCCACTAGC+AGG	+	chr1.2:69146160-69146179	None:intergenic	45.0%
	GATCCCAAACACTCACATGT+TGG	-	chr1.2:69146310-69146329	MS.gene60577:CDS	45.0%
	GTGAAATGGAATAGGGTGAG+TGG	-	chr1.2:69146367-69146386	MS.gene60577:CDS	45.0%
!	AACTCTGTTGGTGGAAACCT+TGG	-	chr1.2:69146214-69146233	MS.gene60577:CDS	45.0%
!	TTTTCTGCTGCCACATCAAG+AGG	-	chr1.2:69146532-69146551	MS.gene60577:CDS	45.0%
!!	ACCCCAACAAATGGATTGTG+AGG	+	chr1.2:69146259-69146278	None:intergenic	45.0%
	ATTCCTGCCAACTCTGTTGG+TGG	-	chr1.2:69146205-69146224	MS.gene60577:CDS	50.0%
	CTTCCGAACACCGTAAGGAT+TGG	-	chr1.2:69146508-69146527	MS.gene60577:CDS	50.0%
	TTTCCACCAACAGAGTTGGC+AGG	+	chr1.2:69146211-69146230	None:intergenic	50.0%
!	TTTGGGATCCCATGAACCAG+TGG	+	chr1.2:69146299-69146318	None:intergenic	50.0%
!!	GCAGGAATATTGGCTCGATC+TGG	+	chr1.2:69146193-69146212	None:intergenic	50.0%
!!	GGAATATTGGCTCGATCTGG+TGG	+	chr1.2:69146190-69146209	None:intergenic	50.0%
	CAGAGCTTCCGAACACCGTA+AGG	-	chr1.2:69146503-69146522	MS.gene60577:CDS	55.0%
	CTGACCGGCACCTCTTGATG+TGG	+	chr1.2:69146545-69146564	None:intergenic	60.0%
	GAATAGGGTGAGTGGCGCCT+TGG	-	chr1.2:69146375-69146394	MS.gene60577:CDS	60.0%
!	GCTGCCACATCAAGAGGTGC+CGG	-	chr1.2:69146538-69146557	MS.gene60577:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	69146151	69146588	69146151	ID=MS.gene60577
chr1.2	mRNA	69146151	69146588	69146151	ID=MS.gene60577.t1;Parent=MS.gene60577
chr1.2	exon	69146151	69146588	69146151	ID=MS.gene60577.t1.exon1;Parent=MS.gene60577.t1
chr1.2	CDS	69146151	69146588	69146151	ID=cds.MS.gene60577.t1;Parent=MS.gene60577.t1

Gene Sequence

>MS.gene60577

ATGACGCCTGCTAGTGGACTTGTCTTCATTCTTACACCACCAGATCGAGCCAATATTCCTGCCAACTCTGTTGGTGGAAACCTTGGAGTTGTTAATGGAAACAGTCCTCACAATCCATTTGTTGGGGTAGAGTTTGATACTAGTTCCACTGGTTCATGGGATCCCAAACACTCACATGTTGGAATTGATGTCAACTCTTTGATTTCTTTAAAGACAGTGAAATGGAATAGGGTGAGTGGCGCCTTGGTGAATGTAAAAATCTCCTATGACAATCTCTCTAAGACATTGAATGTTGTTGTATATTACCCAGATGGTACCTTTTCAACTATTGCTCAAGTCATTGATTTGAAAGCAGAGCTTCCGAACACCGTAAGGATTGGTTTTTCTGCTGCCACATCAAGAGGTGCCGGTCAGCTAAACAACTCTTTTATGGGATAA

Protein sequence

>MS.gene60577.t1

MTPASGLVFILTPPDRANIPANSVGGNLGVVNGNSPHNPFVGVEFDTSSTGSWDPKHSHVGIDVNSLISLKTVKWNRVSGALVNVKISYDNLSKTLNVVVYYPDGTFSTIAQVIDLKAELPNTVRIGFSAATSRGAGQLNNSFMG