Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60615.t1 | XP_003592191.1 | 89.7 | 136 | 12 | 2 | 1 | 134 | 1 | 136 | 1.20E-45 | 192.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60615.t1 | Q9LTE3 | 50.0 | 132 | 50 | 4 | 1 | 132 | 1 | 116 | 6.0e-14 | 78.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60615.t1 | G7IDY9 | 89.7 | 136 | 12 | 2 | 1 | 134 | 1 | 136 | 8.6e-46 | 192.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050961 | MS.gene60615 | 0.806867 | 6.68E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene60615 | 0.80253 | 5.37E-49 | -1.69E-46 |
| MS.gene05410 | MS.gene60615 | 0.805003 | 1.65E-49 | -1.69E-46 |
| MS.gene054892 | MS.gene60615 | 0.858288 | 9.13E-63 | -1.69E-46 |
| MS.gene054893 | MS.gene60615 | 0.85431 | 1.34E-61 | -1.69E-46 |
| MS.gene055226 | MS.gene60615 | 0.802213 | 6.24E-49 | -1.69E-46 |
| MS.gene05624 | MS.gene60615 | 0.817527 | 3.15E-52 | -1.69E-46 |
| MS.gene057622 | MS.gene60615 | 0.80787 | 4.09E-50 | -1.69E-46 |
| MS.gene05952 | MS.gene60615 | 0.803614 | 3.20E-49 | -1.69E-46 |
| MS.gene06023 | MS.gene60615 | 0.804245 | 2.37E-49 | -1.69E-46 |
| MS.gene060257 | MS.gene60615 | 0.812049 | 5.15E-51 | -1.69E-46 |
| MS.gene060412 | MS.gene60615 | 0.834242 | 3.36E-56 | -1.69E-46 |
| MS.gene061047 | MS.gene60615 | 0.836371 | 9.73E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60615.t1 | MTR_1g099800 | 91.912 | 136 | 9 | 2 | 1 | 134 | 1 | 136 | 1.19e-70 | 208 |
| MS.gene60615.t1 | MTR_1g102240 | 85.821 | 134 | 17 | 1 | 1 | 134 | 1 | 132 | 2.10e-61 | 185 |
| MS.gene60615.t1 | MTR_1g099815 | 85.821 | 134 | 17 | 1 | 1 | 134 | 1 | 132 | 8.91e-61 | 183 |
| MS.gene60615.t1 | MTR_1g099810 | 75.714 | 140 | 27 | 5 | 1 | 134 | 1 | 139 | 1.08e-53 | 166 |
| MS.gene60615.t1 | MTR_1g099810 | 74.380 | 121 | 24 | 5 | 20 | 134 | 1 | 120 | 7.98e-42 | 135 |
| MS.gene60615.t1 | MTR_6g049280 | 57.778 | 135 | 43 | 4 | 1 | 133 | 31 | 153 | 9.80e-36 | 120 |
| MS.gene60615.t1 | MTR_6g049280 | 58.519 | 135 | 42 | 4 | 1 | 133 | 1 | 123 | 1.92e-35 | 119 |
| MS.gene60615.t1 | MTR_6g049320 | 57.037 | 135 | 44 | 4 | 1 | 133 | 31 | 153 | 4.48e-34 | 116 |
| MS.gene60615.t1 | MTR_6g049320 | 57.778 | 135 | 43 | 4 | 1 | 133 | 1 | 123 | 7.13e-34 | 115 |
| MS.gene60615.t1 | MTR_6g051690 | 55.556 | 135 | 49 | 4 | 1 | 133 | 1 | 126 | 9.27e-34 | 114 |
| MS.gene60615.t1 | MTR_1g099810 | 73.077 | 104 | 22 | 4 | 36 | 134 | 2 | 104 | 6.30e-33 | 112 |
| MS.gene60615.t1 | MTR_8g098915 | 48.175 | 137 | 56 | 4 | 1 | 133 | 1 | 126 | 3.41e-31 | 108 |
| MS.gene60615.t1 | MTR_1g099640 | 54.478 | 134 | 55 | 3 | 1 | 133 | 1 | 129 | 1.66e-29 | 103 |
| MS.gene60615.t1 | MTR_6g051680 | 68.571 | 70 | 21 | 1 | 1 | 69 | 1 | 70 | 1.98e-29 | 103 |
| MS.gene60615.t1 | MTR_3g434890 | 43.902 | 123 | 50 | 4 | 1 | 119 | 1 | 108 | 2.81e-27 | 97.8 |
| MS.gene60615.t1 | MTR_3g434960 | 43.902 | 123 | 50 | 4 | 1 | 119 | 1 | 108 | 5.01e-27 | 97.1 |
| MS.gene60615.t1 | MTR_7g451210 | 44.000 | 125 | 51 | 4 | 1 | 121 | 1 | 110 | 6.14e-27 | 97.1 |
| MS.gene60615.t1 | MTR_7g451200 | 43.089 | 123 | 56 | 2 | 1 | 122 | 1 | 110 | 6.65e-27 | 97.1 |
| MS.gene60615.t1 | MTR_0795s0020 | 52.518 | 139 | 47 | 5 | 1 | 133 | 1 | 126 | 3.61e-26 | 95.5 |
| MS.gene60615.t1 | MTR_1173s0010 | 58.442 | 77 | 31 | 1 | 1 | 76 | 1 | 77 | 5.04e-26 | 94.7 |
| MS.gene60615.t1 | MTR_6g049260 | 57.895 | 76 | 31 | 1 | 1 | 75 | 1 | 76 | 6.76e-26 | 94.4 |
| MS.gene60615.t1 | MTR_3g435000 | 43.636 | 110 | 46 | 2 | 6 | 114 | 10 | 104 | 1.64e-23 | 88.2 |
| MS.gene60615.t1 | MTR_8g098915 | 44.068 | 118 | 51 | 4 | 20 | 133 | 1 | 107 | 4.60e-21 | 82.0 |
| MS.gene60615.t1 | MTR_7g079110 | 57.353 | 68 | 28 | 1 | 1 | 67 | 1 | 68 | 3.62e-19 | 79.0 |
| MS.gene60615.t1 | MTR_3g434920 | 41.667 | 96 | 37 | 4 | 28 | 119 | 2 | 82 | 1.39e-15 | 67.4 |
| MS.gene60615.t1 | MTR_1g099640 | 50.000 | 98 | 44 | 2 | 36 | 133 | 2 | 94 | 6.52e-13 | 60.8 |
| MS.gene60615.t1 | MTR_3g082250 | 41.975 | 81 | 41 | 4 | 2 | 76 | 4 | 84 | 2.60e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60615.t1 | AT5G52740 | 45.865 | 133 | 54 | 5 | 1 | 132 | 1 | 116 | 1.06e-19 | 79.0 |
| MS.gene60615.t1 | AT5G52760 | 40.288 | 139 | 61 | 6 | 2 | 134 | 4 | 126 | 1.66e-19 | 78.6 |
| MS.gene60615.t1 | AT5G52750 | 49.231 | 65 | 32 | 1 | 3 | 66 | 5 | 69 | 6.70e-13 | 62.0 |
Find 22 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGCAGTCTCTGGCCTTTC+AGG | 0.156873 | 1.2:+69612634 | MS.gene60615:CDS |
| ATTAATCCTTTCATGCTCTA+TGG | 0.308553 | 1.2:+69613075 | MS.gene60615:CDS |
| ATTCATCTGATGATTATAAT+AGG | 0.369814 | 1.2:-69613109 | None:intergenic |
| ACAGAAAATTGTGCTAAAGT+TGG | 0.399453 | 1.2:+69612576 | MS.gene60615:intron |
| TATATGATGACACAGCCATT+AGG | 0.441354 | 1.2:-69613174 | None:intergenic |
| AGCTTAGCCACTACACGTAC+TGG | 0.441404 | 1.2:-69612934 | None:intergenic |
| TGGGTCGGTATCTCCTACTA+AGG | 0.446407 | 1.2:-69612914 | None:intergenic |
| GACAAGAAAATGACCTTAGT+AGG | 0.452020 | 1.2:+69612901 | MS.gene60615:CDS |
| AGGATCCTCTTCAACACTTA+CGG | 0.467744 | 1.2:-69613154 | None:intergenic |
| AAAGCTATGAAGGCAGTCTC+TGG | 0.474866 | 1.2:+69612625 | MS.gene60615:CDS |
| TTCAGTTGGACCAGCTAAAG+AGG | 0.475094 | 1.2:+69612984 | MS.gene60615:CDS |
| GCACGGTAGTATGAATTGTA+TGG | 0.489699 | 1.2:-69613132 | None:intergenic |
| CATGCTGAAATACTTTCAGT+TGG | 0.503143 | 1.2:+69612970 | MS.gene60615:CDS |
| TGATGATTATAATAGGTAGT+AGG | 0.521368 | 1.2:-69613102 | None:intergenic |
| GGTGTACCATAGAGCATGAA+AGG | 0.548293 | 1.2:-69613081 | None:intergenic |
| CCGTGCCGTAAGTGTTGAAG+AGG | 0.551299 | 1.2:+69613149 | MS.gene60615:CDS |
| TACGTGTAGTGGCTAAGCTA+AGG | 0.568064 | 1.2:+69612938 | MS.gene60615:CDS |
| GCTTAGCCACTACACGTACT+GGG | 0.574746 | 1.2:-69612933 | None:intergenic |
| AGTGTTGAAGAGGATCCTAA+TGG | 0.585153 | 1.2:+69613159 | MS.gene60615:CDS |
| TACCGACCCAGTACGTGTAG+TGG | 0.629525 | 1.2:+69612927 | MS.gene60615:CDS |
| AGCCACTACACGTACTGGGT+CGG | 0.643878 | 1.2:-69612929 | None:intergenic |
| CCTCTTCAACACTTACGGCA+CGG | 0.651373 | 1.2:-69613149 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAAGATGATAAAAAGA+AGG | + | chr1.2:69613023-69613042 | MS.gene60615:CDS | 15.0% |
| !!! | TGTATTTTAATAATTTTGCA+GGG | + | chr1.2:69612854-69612873 | MS.gene60615:intron | 15.0% |
| !!! | TTCTTTTTATCATCTTTTTT+AGG | - | chr1.2:69613024-69613043 | None:intergenic | 15.0% |
| !!! | TTGTATTTTAATAATTTTGC+AGG | + | chr1.2:69612853-69612872 | MS.gene60615:intron | 15.0% |
| !! | ATTCATCTGATGATTATAAT+AGG | - | chr1.2:69613112-69613131 | None:intergenic | 20.0% |
| !!! | AAAAAATCTACTTGTTAGAA+CGG | - | chr1.2:69612551-69612570 | None:intergenic | 20.0% |
| !!! | ATTTTTTTTCTTTACCTGAA+AGG | - | chr1.2:69612651-69612670 | None:intergenic | 20.0% |
| !!! | GTATTTTAATAATTTTGCAG+GGG | + | chr1.2:69612855-69612874 | MS.gene60615:intron | 20.0% |
| ! | AATCAAACAAAAAGCTATGA+AGG | + | chr1.2:69612615-69612634 | MS.gene60615:CDS | 25.0% |
| ! | ATCGAACTTAGTCTAATATA+CGG | - | chr1.2:69612742-69612761 | None:intergenic | 25.0% |
| ! | TATCATAGCTAAAGTATGAT+TGG | + | chr1.2:69612428-69612447 | MS.gene60615:intron | 25.0% |
| ! | TCATACTTTAGCTATGATAT+AGG | - | chr1.2:69612427-69612446 | None:intergenic | 25.0% |
| ! | TGATGATTATAATAGGTAGT+AGG | - | chr1.2:69613105-69613124 | None:intergenic | 25.0% |
| ! | TTGAAATTGTGCATAAGAAA+AGG | - | chr1.2:69612462-69612481 | None:intergenic | 25.0% |
| !!! | AATCATTGATTCTACGAATT+TGG | - | chr1.2:69612798-69612817 | None:intergenic | 25.0% |
| !!! | ACTAAGTTCGATATTTTGAT+TGG | + | chr1.2:69612750-69612769 | MS.gene60615:intron | 25.0% |
| !!! | TAAAAGAGTGGTTTTAATCA+AGG | - | chr1.2:69612401-69612420 | None:intergenic | 25.0% |
| ACAGAAAATTGTGCTAAAGT+TGG | + | chr1.2:69612576-69612595 | MS.gene60615:intron | 30.0% | |
| ATTAATCCTTTCATGCTCTA+TGG | + | chr1.2:69613075-69613094 | MS.gene60615:CDS | 30.0% | |
| TGATATAGGAGATAAAAGAG+TGG | - | chr1.2:69612413-69612432 | None:intergenic | 30.0% | |
| CATGCTGAAATACTTTCAGT+TGG | + | chr1.2:69612970-69612989 | MS.gene60615:CDS | 35.0% | |
| GACAAGAAAATGACCTTAGT+AGG | + | chr1.2:69612901-69612920 | MS.gene60615:CDS | 35.0% | |
| TATATGATGACACAGCCATT+AGG | - | chr1.2:69613177-69613196 | None:intergenic | 35.0% | |
| TTGATTGGACAATTCAGATG+TGG | + | chr1.2:69612765-69612784 | MS.gene60615:intron | 35.0% | |
| ! | TACCAAAGTGCTTCAAATTC+AGG | - | chr1.2:69612486-69612505 | None:intergenic | 35.0% |
| ! | TCTTTCTTTTCCTCTTTAGC+TGG | - | chr1.2:69612997-69613016 | None:intergenic | 35.0% |
| AGGATCCTCTTCAACACTTA+CGG | - | chr1.2:69613157-69613176 | None:intergenic | 40.0% | |
| GCACGGTAGTATGAATTGTA+TGG | - | chr1.2:69613135-69613154 | None:intergenic | 40.0% | |
| ! | AGCCTGAATTTGAAGCACTT+TGG | + | chr1.2:69612481-69612500 | MS.gene60615:intron | 40.0% |
| ! | AGTGTTGAAGAGGATCCTAA+TGG | + | chr1.2:69613159-69613178 | MS.gene60615:CDS | 40.0% |
| AAAGCTATGAAGGCAGTCTC+TGG | + | chr1.2:69612625-69612644 | MS.gene60615:CDS | 45.0% | |
| GGTGTACCATAGAGCATGAA+AGG | - | chr1.2:69613084-69613103 | None:intergenic | 45.0% | |
| TACGTGTAGTGGCTAAGCTA+AGG | + | chr1.2:69612938-69612957 | MS.gene60615:CDS | 45.0% | |
| TTCAGTTGGACCAGCTAAAG+AGG | + | chr1.2:69612984-69613003 | MS.gene60615:CDS | 45.0% | |
| AGCTTAGCCACTACACGTAC+TGG | - | chr1.2:69612937-69612956 | None:intergenic | 50.0% | |
| CCTCTTCAACACTTACGGCA+CGG | - | chr1.2:69613152-69613171 | None:intergenic | 50.0% | |
| GCTTAGCCACTACACGTACT+GGG | - | chr1.2:69612936-69612955 | None:intergenic | 50.0% | |
| TGGGTCGGTATCTCCTACTA+AGG | - | chr1.2:69612917-69612936 | None:intergenic | 50.0% | |
| AGCCACTACACGTACTGGGT+CGG | - | chr1.2:69612932-69612951 | None:intergenic | 55.0% | |
| TACCGACCCAGTACGTGTAG+TGG | + | chr1.2:69612927-69612946 | MS.gene60615:CDS | 55.0% | |
| ! | AAGGCAGTCTCTGGCCTTTC+AGG | + | chr1.2:69612634-69612653 | MS.gene60615:CDS | 55.0% |
| !! | CCGTGCCGTAAGTGTTGAAG+AGG | + | chr1.2:69613149-69613168 | MS.gene60615:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 69612376 | 69613197 | 69612376 | ID=MS.gene60615 |
| chr1.2 | mRNA | 69612376 | 69613197 | 69612376 | ID=MS.gene60615.t1;Parent=MS.gene60615 |
| chr1.2 | exon | 69612376 | 69612381 | 69612376 | ID=MS.gene60615.t1.exon1;Parent=MS.gene60615.t1 |
| chr1.2 | CDS | 69612376 | 69612381 | 69612376 | ID=cds.MS.gene60615.t1;Parent=MS.gene60615.t1 |
| chr1.2 | exon | 69612580 | 69612655 | 69612580 | ID=MS.gene60615.t1.exon2;Parent=MS.gene60615.t1 |
| chr1.2 | CDS | 69612580 | 69612655 | 69612580 | ID=cds.MS.gene60615.t1;Parent=MS.gene60615.t1 |
| chr1.2 | exon | 69612875 | 69613197 | 69612875 | ID=MS.gene60615.t1.exon3;Parent=MS.gene60615.t1 |
| chr1.2 | CDS | 69612875 | 69613197 | 69612875 | ID=cds.MS.gene60615.t1;Parent=MS.gene60615.t1 |
| Gene Sequence |
| Protein sequence |
