AlfalfaGEDB | MS.gene60615

M. sativa cultivar XinJiangDaYe / MS.gene60615

KEGG: -
GO: GO:0003674 GO:0005488 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0030001 GO:0043167 GO:0043169 GO:0046872 GO:0051179 GO:0051234
PFAM: PF00403.29

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60615.t1	XP_003592191.1	89.7	136	12	2	1	134	1	136	1.20E-45	192.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60615.t1	Q9LTE3	50.0	132	50	4	1	132	1	116	6.0e-14	78.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60615.t1	G7IDY9	89.7	136	12	2	1	134	1	136	8.6e-46	192.6

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050961	MS.gene60615	0.806867	6.68E-50	-1.69E-46
MS.gene053963	MS.gene60615	0.80253	5.37E-49	-1.69E-46
MS.gene05410	MS.gene60615	0.805003	1.65E-49	-1.69E-46
MS.gene054892	MS.gene60615	0.858288	9.13E-63	-1.69E-46
MS.gene054893	MS.gene60615	0.85431	1.34E-61	-1.69E-46
MS.gene055226	MS.gene60615	0.802213	6.24E-49	-1.69E-46
MS.gene05624	MS.gene60615	0.817527	3.15E-52	-1.69E-46
MS.gene057622	MS.gene60615	0.80787	4.09E-50	-1.69E-46
MS.gene05952	MS.gene60615	0.803614	3.20E-49	-1.69E-46
MS.gene06023	MS.gene60615	0.804245	2.37E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60615.t1	MTR_1g099800	91.912	136	9	2	1	134	1	136	1.19e-70	208
MS.gene60615.t1	MTR_1g102240	85.821	134	17	1	1	134	1	132	2.10e-61	185
MS.gene60615.t1	MTR_1g099815	85.821	134	17	1	1	134	1	132	8.91e-61	183
MS.gene60615.t1	MTR_1g099810	75.714	140	27	5	1	134	1	139	1.08e-53	166
MS.gene60615.t1	MTR_1g099810	74.380	121	24	5	20	134	1	120	7.98e-42	135
MS.gene60615.t1	MTR_6g049280	57.778	135	43	4	1	133	31	153	9.80e-36	120
MS.gene60615.t1	MTR_6g049280	58.519	135	42	4	1	133	1	123	1.92e-35	119
MS.gene60615.t1	MTR_6g049320	57.037	135	44	4	1	133	31	153	4.48e-34	116
MS.gene60615.t1	MTR_6g049320	57.778	135	43	4	1	133	1	123	7.13e-34	115
MS.gene60615.t1	MTR_6g051690	55.556	135	49	4	1	133	1	126	9.27e-34	114

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60615.t1	AT5G52740	45.865	133	54	5	1	132	1	116	1.06e-19	79.0
MS.gene60615.t1	AT5G52760	40.288	139	61	6	2	134	4	126	1.66e-19	78.6
MS.gene60615.t1	AT5G52750	49.231	65	32	1	3	66	5	69	6.70e-13	62.0

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Find 22 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCTATGAAGGCAGTCTC+TGG	0.474866	1.2:+69612625	MS.gene60615:CDS
AAGGCAGTCTCTGGCCTTTC+AGG	0.156873	1.2:+69612634	MS.gene60615:CDS
ACAGAAAATTGTGCTAAAGT+TGG	0.399453	1.2:+69612576	MS.gene60615:intron
AGCCACTACACGTACTGGGT+CGG	0.643878	1.2:-69612929	None:intergenic
AGCTTAGCCACTACACGTAC+TGG	0.441404	1.2:-69612934	None:intergenic
AGGATCCTCTTCAACACTTA+CGG	0.467744	1.2:-69613154	None:intergenic
AGTGTTGAAGAGGATCCTAA+TGG	0.585153	1.2:+69613159	MS.gene60615:CDS
ATTAATCCTTTCATGCTCTA+TGG	0.308553	1.2:+69613075	MS.gene60615:CDS
ATTCATCTGATGATTATAAT+AGG	0.369814	1.2:-69613109	None:intergenic
CATGCTGAAATACTTTCAGT+TGG	0.503143	1.2:+69612970	MS.gene60615:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAGAAAATTGTGCTAAAGT+TGG	+	chr1.2:69612576-69612595	MS.gene60615:intron	30.0%
ATTAATCCTTTCATGCTCTA+TGG	+	chr1.2:69613075-69613094	MS.gene60615:CDS	30.0%
TGATATAGGAGATAAAAGAG+TGG	-	chr1.2:69612413-69612432	None:intergenic	30.0%
CATGCTGAAATACTTTCAGT+TGG	+	chr1.2:69612970-69612989	MS.gene60615:CDS	35.0%
GACAAGAAAATGACCTTAGT+AGG	+	chr1.2:69612901-69612920	MS.gene60615:CDS	35.0%
TATATGATGACACAGCCATT+AGG	-	chr1.2:69613177-69613196	None:intergenic	35.0%
TTGATTGGACAATTCAGATG+TGG	+	chr1.2:69612765-69612784	MS.gene60615:intron	35.0%
AGGATCCTCTTCAACACTTA+CGG	-	chr1.2:69613157-69613176	None:intergenic	40.0%
GCACGGTAGTATGAATTGTA+TGG	-	chr1.2:69613135-69613154	None:intergenic	40.0%
AAAGCTATGAAGGCAGTCTC+TGG	+	chr1.2:69612625-69612644	MS.gene60615:CDS	45.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	69612376	69613197	69612376	ID=MS.gene60615
chr1.2	mRNA	69612376	69613197	69612376	ID=MS.gene60615.t1;Parent=MS.gene60615
chr1.2	exon	69612376	69612381	69612376	ID=MS.gene60615.t1.exon1;Parent=MS.gene60615.t1
chr1.2	CDS	69612376	69612381	69612376	ID=cds.MS.gene60615.t1;Parent=MS.gene60615.t1
chr1.2	exon	69612580	69612655	69612580	ID=MS.gene60615.t1.exon2;Parent=MS.gene60615.t1
chr1.2	CDS	69612580	69612655	69612580	ID=cds.MS.gene60615.t1;Parent=MS.gene60615.t1
chr1.2	exon	69612875	69613197	69612875	ID=MS.gene60615.t1.exon3;Parent=MS.gene60615.t1
chr1.2	CDS	69612875	69613197	69612875	ID=cds.MS.gene60615.t1;Parent=MS.gene60615.t1

Gene Sequence

>MS.gene60615

ATGAAGGTTAGTTAATACTTTACCTTGATTAAAACCACTCTTTTATCTCCTATATCATAGCTAAAGTATGATTGGTGAATTTTCCTTTTCTTATGCACAATTTCAAGCCTGAATTTGAAGCACTTTGGTAATCTTAGTTTGAGACTTAAAATTTAGAAAGACAGTGAGTGTTCCGTTCTAACAAGTAGATTTTTTCTTTGACAGAAAATTGTGCTAAAGTTGGAGATTCATGAAGACAAAATCAAACAAAAAGCTATGAAGGCAGTCTCTGGCCTTTCAGGTAAAGAAAAAAAATTCTATTGATCATGTAGCATCAATATCAATGCTGTGTGCAGTCAAAATCTCATGACTGCACACAGTTAGCCGTATATTAGACTAAGTTCGATATTTTGATTGGACAATTCAGATGTGGCAACAATCCAAATTCGTAGAATCAATGATTTGTCGATTTTTATTTTAGTGATATATATACTAAGATTGTATTTTAATAATTTTGCAGGGGTTGAGTCAGTTGAAGTAGACAAAGACAAGAAAATGACCTTAGTAGGAGATACCGACCCAGTACGTGTAGTGGCTAAGCTAAGGAAGATTTGTCATGCTGAAATACTTTCAGTTGGACCAGCTAAAGAGGAAAAGAAAGATGAACCTAAAAAAGATGATAAAAAGAAGGAAGATGATAAGAAAGATTCAACAATGATAATTAATCCTTTCATGCTCTATGGTACACCTACTACCTATTATAATCATCAGATGAATCCATACAATTCATACTACCGTGCCGTAAGTGTTGAAGAGGATCCTAATGGCTGTGTCATCATATAA

Protein sequence

>MS.gene60615.t1

MKKIVLKLEIHEDKIKQKAMKAVSGLSGVESVEVDKDKKMTLVGDTDPVRVVAKLRKICHAEILSVGPAKEEKKDEPKKDDKKKEDDKKDSTMIINPFMLYGTPTTYYNHQMNPYNSYYRAVSVEEDPNGCVII