AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60804

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60804.t1 MTR_1g096310 90.860 372 5 1 1 343 1 372 0.0 684
MS.gene60804.t1 MTR_7g011900 58.713 373 121 4 1 343 1 370 2.49e-152 433
MS.gene60804.t1 MTR_7g012040 58.082 365 121 3 8 343 7 368 1.98e-147 420
MS.gene60804.t1 MTR_7g012020 56.011 366 128 4 8 343 7 369 2.24e-142 407
MS.gene60804.t1 MTR_7g011990 54.496 367 129 4 8 343 8 367 1.28e-137 395
MS.gene60804.t1 MTR_7g012070 52.394 376 145 4 1 343 1 375 6.23e-135 389
MS.gene60804.t1 MTR_7g012090 52.926 376 143 4 1 343 1 375 1.85e-134 387
MS.gene60804.t1 MTR_2g055940 55.241 353 124 4 21 343 15 363 1.17e-133 385
MS.gene60804.t1 MTR_3g021430 51.247 361 142 5 13 343 1 357 6.21e-119 347
MS.gene60804.t1 MTR_3g021440 50.708 353 140 5 21 343 22 370 1.48e-116 342
MS.gene60804.t1 MTR_1g036460 46.486 370 161 7 7 343 5 370 2.29e-108 321
MS.gene60804.t1 MTR_7g012020 59.041 271 104 4 8 277 7 271 8.19e-108 317
MS.gene60804.t1 MTR_7g012020 62.857 245 87 3 8 251 7 248 2.57e-107 314
MS.gene60804.t1 MTR_1g036490 45.380 368 165 6 9 343 6 370 7.26e-107 317
MS.gene60804.t1 MTR_1g036510 45.308 373 160 7 12 343 15 384 6.49e-103 308
MS.gene60804.t1 MTR_3g092900 43.956 364 168 7 10 343 6 363 9.74e-95 286
MS.gene60804.t1 MTR_5g098170 39.831 354 179 6 20 343 14 363 2.88e-78 244
MS.gene60804.t1 MTR_1g050220 40.867 323 128 6 26 326 6 287 8.99e-73 228
MS.gene60804.t1 MTR_1g036510 48.193 249 115 5 12 249 15 260 2.25e-69 218
MS.gene60804.t1 MTR_7g118300 37.838 370 186 8 8 343 16 375 3.92e-69 221
MS.gene60804.t1 MTR_1g036510 48.193 249 115 5 12 249 15 260 8.05e-69 218
MS.gene60804.t1 MTR_0591s0010 36.011 361 189 9 18 343 24 377 4.01e-63 205
MS.gene60804.t1 MTR_7g118320 34.605 367 196 9 13 343 4 362 7.26e-63 204
MS.gene60804.t1 MTR_8g068690 35.097 359 195 8 18 343 23 376 8.47e-61 199
MS.gene60804.t1 MTR_8g068750 34.540 359 197 8 18 343 24 377 7.77e-60 197
MS.gene60804.t1 MTR_8g068870 33.880 366 204 8 11 343 3 363 3.23e-55 184
MS.gene60804.t1 MTR_8g024160 31.549 355 167 6 19 343 20 328 3.52e-49 167
MS.gene60804.t1 MTR_6g078850 31.635 373 218 7 3 342 14 382 3.15e-48 169
MS.gene60804.t1 MTR_3g096050 32.800 375 194 12 9 343 2 358 5.43e-46 160
MS.gene60804.t1 MTR_8g068570 33.129 326 154 8 18 310 24 318 1.21e-44 155
MS.gene60804.t1 MTR_6g060290 47.802 182 73 6 8 186 8 170 2.66e-44 150
MS.gene60804.t1 MTR_8g068690 37.872 235 139 5 18 249 23 253 3.77e-44 153
MS.gene60804.t1 MTR_2g072790 30.460 348 200 7 13 327 1 339 4.40e-44 155
MS.gene60804.t1 MTR_8g068730 30.919 359 178 9 18 343 24 345 1.18e-42 151
MS.gene60804.t1 MTR_8g068600 37.073 205 123 4 47 249 28 228 5.06e-37 134
MS.gene60804.t1 MTR_3g096070 31.200 375 177 15 9 343 2 335 1.26e-35 132
MS.gene60804.t1 MTR_3g096050 35.918 245 141 7 9 249 2 234 6.39e-34 126
MS.gene60804.t1 MTR_0017s0100 30.275 327 173 15 41 328 44 354 3.06e-30 118
MS.gene60804.t1 MTR_0079s0070 29.721 323 180 11 41 328 10 320 6.40e-30 117
MS.gene60804.t1 MTR_5g074680 27.410 332 194 9 34 329 27 347 1.05e-29 117
MS.gene60804.t1 MTR_5g074600 25.602 332 200 10 34 329 26 346 1.12e-29 117
MS.gene60804.t1 MTR_4g038440 29.721 323 180 11 41 328 44 354 1.50e-29 116
MS.gene60804.t1 MTR_3g083620 26.006 323 193 8 39 328 38 347 7.32e-27 109
MS.gene60804.t1 MTR_5g074580 26.205 332 198 10 34 329 27 347 2.20e-26 107
MS.gene60804.t1 MTR_5g074540 26.377 345 188 12 34 342 26 340 3.70e-26 107
MS.gene60804.t1 MTR_8g068740 28.814 295 165 8 18 309 24 276 9.47e-25 102
MS.gene60804.t1 MTR_4g088345 26.524 328 189 10 38 328 30 342 1.59e-24 102
MS.gene60804.t1 MTR_7g014510 25.234 321 199 8 41 329 33 344 2.87e-24 102
MS.gene60804.t1 MTR_8g068620 51.190 84 41 0 166 249 2 85 4.74e-24 95.5
MS.gene60804.t1 MTR_4g033200 27.247 356 212 10 13 328 1 349 1.44e-23 100
MS.gene60804.t1 MTR_1g076940 25.309 324 197 7 41 328 33 347 2.37e-23 99.8
MS.gene60804.t1 MTR_4g079160 26.271 354 201 14 19 328 4 341 3.64e-23 99.0
MS.gene60804.t1 MTR_4g088200 28.024 339 200 10 34 336 26 356 5.22e-23 98.6
MS.gene60804.t1 MTR_4g033015 26.685 356 214 9 13 328 1 349 8.33e-23 98.2
MS.gene60804.t1 MTR_4g088340 25.610 328 192 10 38 328 7 319 9.26e-22 94.7
MS.gene60804.t1 MTR_7g024500 25.000 328 189 9 38 328 29 336 1.43e-21 94.4
MS.gene60804.t1 MTR_7g024520 25.000 332 192 9 34 328 25 336 2.34e-21 94.0
MS.gene60804.t1 MTR_7g014570 25.000 324 191 9 41 329 33 339 1.81e-20 91.3
MS.gene60804.t1 MTR_4g033150 25.779 353 215 9 16 328 4 349 2.03e-20 91.3
MS.gene60804.t1 MTR_5g074610 23.952 334 172 11 34 328 22 312 2.71e-19 87.4
MS.gene60804.t1 MTR_4g038440 31.776 214 129 9 41 249 44 245 7.66e-19 85.5
MS.gene60804.t1 MTR_0794s0020 26.877 253 167 8 16 260 4 246 1.96e-17 80.9
MS.gene60804.t1 MTR_1g076930 26.047 215 144 6 38 248 7 210 2.29e-16 77.8
MS.gene60804.t1 MTR_4g088190 27.326 344 199 10 34 336 26 359 1.39e-15 77.4
MS.gene60804.t1 MTR_4g033170 26.211 351 208 10 17 328 5 343 2.23e-14 73.6
MS.gene60804.t1 MTR_8g068760 38.710 93 55 2 252 343 142 233 1.44e-13 69.7
MS.gene60804.t1 MTR_4g033085 26.496 351 207 10 17 328 5 343 8.98e-13 68.9
MS.gene60804.t1 MTR_8g068580 40.217 92 53 2 253 343 43 133 1.28e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60804.t1 AT5G54160 42.733 344 161 5 20 333 15 352 8.73e-88 268
MS.gene60804.t1 AT1G51990 36.364 363 193 7 12 342 5 361 1.85e-70 224
MS.gene60804.t1 AT1G51990 36.364 363 193 7 12 342 5 361 1.61e-69 221
MS.gene60804.t1 AT1G77520 35.561 374 196 9 6 343 17 381 1.29e-66 214
MS.gene60804.t1 AT1G33030 36.000 350 181 9 28 343 10 350 6.39e-60 196
MS.gene60804.t1 AT1G63140 35.376 359 193 9 21 343 26 381 1.10e-57 191
MS.gene60804.t1 AT1G63140 35.376 359 193 9 21 343 46 401 1.94e-57 191
MS.gene60804.t1 AT1G77530 36.096 374 194 9 6 343 17 381 2.66e-55 185
MS.gene60804.t1 AT1G21130 35.411 353 187 10 26 343 27 373 1.74e-54 183
MS.gene60804.t1 AT1G21110 35.127 353 188 10 26 343 27 373 3.91e-53 179
MS.gene60804.t1 AT1G21100 35.127 353 188 10 26 343 27 373 1.22e-52 178
MS.gene60804.t1 AT5G53810 35.088 342 184 7 38 343 39 378 6.88e-52 176
MS.gene60804.t1 AT1G21120 34.561 353 190 10 26 343 27 373 1.28e-51 175
MS.gene60804.t1 AT1G21120 34.278 353 191 10 26 343 75 421 3.40e-51 176
MS.gene60804.t1 AT1G76790 33.333 357 191 9 26 343 17 365 2.27e-45 159
MS.gene60804.t1 AT3G53140 30.946 349 194 8 17 327 5 344 4.69e-43 152
MS.gene60804.t1 AT1G33030 36.508 252 149 6 28 277 10 252 7.01e-42 147
MS.gene60804.t1 AT1G33030 36.508 252 149 6 28 277 10 252 9.68e-42 147
MS.gene60804.t1 AT5G37170 33.129 326 146 10 56 343 43 334 1.43e-40 145
MS.gene60804.t1 AT1G63140 37.295 244 140 6 21 253 46 287 8.66e-39 139
MS.gene60804.t1 AT1G77530 38.554 249 140 5 6 249 17 257 3.24e-38 137
MS.gene60804.t1 AT1G63140 37.179 234 140 5 21 249 46 277 6.20e-38 137
MS.gene60804.t1 AT1G21130 40.351 228 127 6 26 249 27 249 7.41e-36 132
MS.gene60804.t1 AT1G62900 37.931 203 94 4 172 343 4 205 1.00e-34 126
MS.gene60804.t1 AT5G37170 37.374 198 91 5 178 343 38 234 5.36e-32 120
MS.gene60804.t1 AT5G37170 37.433 187 84 5 189 343 1 186 4.02e-29 111
MS.gene60804.t1 AT4G35160 27.446 368 203 13 9 328 16 367 2.62e-24 102
MS.gene60804.t1 AT4G35150 30.734 218 102 8 162 336 105 316 7.50e-24 100

Find 68 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCTTTATTAATGGATATT+TGG 0.122440 1.2:+72240979 None:intergenic
AAGTTAATTCCCTTTATTAA+TGG 0.172707 1.2:+72240971 None:intergenic
TCACTTGTGTTTGGTTCTTC+TGG 0.194077 1.2:+72240474 None:intergenic
TGATGAAAGTAGAGGTTATT+TGG 0.221768 1.2:-72241547 MS.gene60804:CDS
GAAAATGCACCACCACTTTC+AGG 0.245970 1.2:-72240929 MS.gene60804:intron
ACGGCAAGCAACTTGAAATT+TGG 0.263471 1.2:+72240344 None:intergenic
ATCCAAATATCCATTAATAA+AGG 0.266045 1.2:-72240981 MS.gene60804:CDS
TGCCGTGCTTTCAACAGTTT+GGG 0.308799 1.2:-72240327 MS.gene60804:CDS
TTGCCGTGCTTTCAACAGTT+TGG 0.318040 1.2:-72240328 MS.gene60804:CDS
TTAGATTCTTCACTTGTGTT+TGG 0.326373 1.2:+72240465 None:intergenic
ATCCTGCATTGTTACAAGTT+TGG 0.339882 1.2:-72241500 MS.gene60804:intron
GACTTGTTCAAGAACGTTCA+TGG 0.340035 1.2:-72241184 MS.gene60804:CDS
GTTCTGAGGTAGCACTAATT+TGG 0.352301 1.2:+72241889 None:intergenic
AGCATCCGGTCAAGCCTATT+AGG 0.381108 1.2:+72241678 None:intergenic
GGTGGTGCCGAGAGAGTTTA+CGG 0.386591 1.2:-72241600 MS.gene60804:CDS
AGCACTCGGACTTGCCTAAT+AGG 0.390804 1.2:-72241692 MS.gene60804:CDS
AGTGACAAAGTATGAATTCA+TGG 0.400109 1.2:-72241161 MS.gene60804:CDS
GTTATCCTCCTTGGTAATGT+AGG 0.401834 1.2:+72241911 None:intergenic
ACTAATTTGGTTATCCTCCT+TGG 0.402910 1.2:+72241902 None:intergenic
TCCAAATATCCATTAATAAA+GGG 0.404369 1.2:-72240980 MS.gene60804:CDS
TGAAATATACACTGGATTTG+AGG 0.429712 1.2:-72241062 MS.gene60804:CDS
CACAAAGCTTTATCACCAAA+TGG 0.430052 1.2:-72240525 MS.gene60804:CDS
AAATAACCTCTACTTTCATC+AGG 0.441916 1.2:+72241549 None:intergenic
GAGAAATTGAGCAAACTCTC+TGG 0.445194 1.2:-72240372 MS.gene60804:CDS
GAAATATACACTGGATTTGA+GGG 0.446533 1.2:-72241061 MS.gene60804:CDS
GTTGATGTTGGAGGTGGAAG+TGG 0.451719 1.2:-72241025 MS.gene60804:CDS
ATAGCAGCATTTAACACTGC+TGG 0.468445 1.2:+72241810 None:intergenic
CTCATGTTTATCACAGTTGG+TGG 0.480360 1.2:-72240420 MS.gene60804:CDS
ATATCAACATTAGTTGATGT+TGG 0.501358 1.2:-72241037 MS.gene60804:CDS
ACATTAGTTGATGTTGGAGG+TGG 0.504612 1.2:-72241031 MS.gene60804:CDS
GAGAGTTTACGGACTCTCAA+TGG 0.504711 1.2:-72241589 MS.gene60804:CDS
AATCTCATGTTTATCACAGT+TGG 0.506086 1.2:-72240423 MS.gene60804:CDS
CTCGGCACCACCATCCTCAA+TGG 0.508530 1.2:+72241611 None:intergenic
TGCTGGATAAACAAGATTAG+TGG 0.509486 1.2:+72241827 None:intergenic
TTTATTTGAGATCATAGCTA+AGG 0.511778 1.2:-72241778 MS.gene60804:CDS
TAGCAAGCAAACGCAGCATC+CGG 0.515114 1.2:+72241664 None:intergenic
TTTCAACAGTTTGGGAGTGA+TGG 0.524643 1.2:-72240319 MS.gene60804:CDS
TGTTTATCACAGTTGGTGGA+AGG 0.529690 1.2:-72240416 MS.gene60804:CDS
TCCGAGTGCTGCGTTGATGC+TGG 0.530284 1.2:+72241705 None:intergenic
GTTTATCACAGTTGGTGGAA+GGG 0.544261 1.2:-72240415 MS.gene60804:CDS
ATCCTCAATGGTTTGAGTAG+TGG 0.545005 1.2:+72241623 None:intergenic
ACTTGCCTAATAGGCTTGAC+CGG 0.546431 1.2:-72241683 MS.gene60804:CDS
TACCAAACTTGTAACAATGC+AGG 0.552486 1.2:+72241498 None:intergenic
AAAGTATGAATTCATGGGAA+AGG 0.556043 1.2:-72241155 MS.gene60804:CDS
ACCAGCATCAACGCAGCACT+CGG 0.557596 1.2:-72241706 MS.gene60804:CDS
TATCATGTTATACCTGAAAG+TGG 0.563244 1.2:+72240917 None:intergenic
GTGACAAAGTATGAATTCAT+GGG 0.563593 1.2:-72241160 MS.gene60804:CDS
AATTTAGAAGCAATTTCTGA+TGG 0.564927 1.2:+72241729 None:intergenic
TTCCACTACTCAAACCATTG+AGG 0.566219 1.2:-72241625 MS.gene60804:CDS
ATAGCTAAGGCAACACCACC+TGG 0.567142 1.2:-72241765 MS.gene60804:CDS
TCAACATTAGTTGATGTTGG+AGG 0.568137 1.2:-72241034 MS.gene60804:CDS
CTCCCAAACTGTTGAAAGCA+CGG 0.568203 1.2:+72240325 None:intergenic
TGAGAGTCCGTAAACTCTCT+CGG 0.571590 1.2:+72241593 None:intergenic
AGAATGCTTGAAATATACAC+TGG 0.575900 1.2:-72241070 MS.gene60804:CDS
CTCTACTTTCATCAGGGACA+AGG 0.594593 1.2:+72241556 None:intergenic
ACTGTCTTCAGTTTGTTCTG+AGG 0.597743 1.2:+72241875 None:intergenic
CAGTGCATGTCTTTCAGCAA+TGG 0.602261 1.2:-72241856 MS.gene60804:CDS
CTTGTCCCTGATGAAAGTAG+AGG 0.618925 1.2:-72241555 MS.gene60804:CDS
AAATGGAAAAGTGATTATTG+TGG 0.620942 1.2:-72240508 MS.gene60804:CDS
GTTTACGGACTCTCAATGGT+CGG 0.636343 1.2:-72241585 MS.gene60804:CDS
ACTACTCAAACCATTGAGGA+TGG 0.637452 1.2:-72241621 MS.gene60804:CDS
GGGAAATTCCTACATTACCA+AGG 0.642464 1.2:-72241919 MS.gene60804:CDS
AATAACCTCTACTTTCATCA+GGG 0.648899 1.2:+72241550 None:intergenic
ACTCAAACCATTGAGGATGG+TGG 0.675966 1.2:-72241618 MS.gene60804:CDS
GATGGTGACATGAAAGCACC+AGG 0.677429 1.2:+72241747 None:intergenic
AAATTCCTACATTACCAAGG+AGG 0.730361 1.2:-72241916 MS.gene60804:CDS
CATGTTATACCTGAAAGTGG+TGG 0.738842 1.2:+72240920 None:intergenic
GGTGACATGAAAGCACCAGG+TGG 0.810388 1.2:+72241750 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATACATATATAAAATCAA+GGG - chr1.2:72240771-72240790 MS.gene60804:intron 15.0%
!! AAGTTAATTCCCTTTATTAA+TGG + chr1.2:72241258-72241277 None:intergenic 20.0%
!! AGTAAAACTTTAGTATAAGA+TGG + chr1.2:72241373-72241392 None:intergenic 20.0%
!! ATATTCACCTATTAAACATA+TGG - chr1.2:72240823-72240842 MS.gene60804:intron 20.0%
!! ATCCAAATATCCATTAATAA+AGG - chr1.2:72241245-72241264 MS.gene60804:intron 20.0%
!! CTCATACATATATAAAATCA+AGG - chr1.2:72240770-72240789 MS.gene60804:intron 20.0%
!! TCCAAATATCCATTAATAAA+GGG - chr1.2:72241246-72241265 MS.gene60804:intron 20.0%
!!! CCAAATTTTTAACAAATCAA+TGG - chr1.2:72241107-72241126 MS.gene60804:CDS 20.0%
!!! CCATTGATTTGTTAAAAATT+TGG + chr1.2:72241110-72241129 None:intergenic 20.0%
! AAATGGAAAAGTGATTATTG+TGG - chr1.2:72241718-72241737 MS.gene60804:CDS 25.0%
! AATTTAGAAGCAATTTCTGA+TGG + chr1.2:72240500-72240519 None:intergenic 25.0%
! ATATCCTCCATATGTTTAAT+AGG + chr1.2:72240833-72240852 None:intergenic 25.0%
! GGAGGATATATAAGTTATAA+AGG - chr1.2:72240844-72240863 MS.gene60804:intron 25.0%
!! ATATCAACATTAGTTGATGT+TGG - chr1.2:72241189-72241208 MS.gene60804:CDS 25.0%
!! ATTTTTCATCTGACCAATTA+TGG + chr1.2:72241658-72241677 None:intergenic 25.0%
!! TCCCTTTATTAATGGATATT+TGG + chr1.2:72241250-72241269 None:intergenic 25.0%
!! TTTATTTGAGATCATAGCTA+AGG - chr1.2:72240448-72240467 MS.gene60804:CDS 25.0%
!!! ACAATAATCACTTTTCCATT+TGG + chr1.2:72241719-72241738 None:intergenic 25.0%
!!! TCTTACTTTTGTTCCAATTA+TGG - chr1.2:72240959-72240978 MS.gene60804:CDS 25.0%
AAAGTATGAATTCATGGGAA+AGG - chr1.2:72241071-72241090 MS.gene60804:CDS 30.0%
AAATAACCTCTACTTTCATC+AGG + chr1.2:72240680-72240699 None:intergenic 30.0%
AATAACCTCTACTTTCATCA+GGG + chr1.2:72240679-72240698 None:intergenic 30.0%
AATCTCATGTTTATCACAGT+TGG - chr1.2:72241803-72241822 MS.gene60804:CDS 30.0%
ACTAAAGCTTAGACCATAAT+TGG + chr1.2:72240975-72240994 None:intergenic 30.0%
AGAATGCTTGAAATATACAC+TGG - chr1.2:72241156-72241175 MS.gene60804:CDS 30.0%
AGTGACAAAGTATGAATTCA+TGG - chr1.2:72241065-72241084 MS.gene60804:CDS 30.0%
GAAATATACACTGGATTTGA+GGG - chr1.2:72241165-72241184 MS.gene60804:CDS 30.0%
GTGACAAAGTATGAATTCAT+GGG - chr1.2:72241066-72241085 MS.gene60804:CDS 30.0%
TATACTTACCTTCAGTATCA+TGG + chr1.2:72241499-72241518 None:intergenic 30.0%
TATCATGTTATACCTGAAAG+TGG + chr1.2:72241312-72241331 None:intergenic 30.0%
TGAAATATACACTGGATTTG+AGG - chr1.2:72241164-72241183 MS.gene60804:CDS 30.0%
TGATGAAAGTAGAGGTTATT+TGG - chr1.2:72240679-72240698 MS.gene60804:intron 30.0%
TTAAGCTAATTGTAGCAATC+AGG - chr1.2:72240929-72240948 MS.gene60804:intron 30.0%
TTAGATTCTTCACTTGTGTT+TGG + chr1.2:72241764-72241783 None:intergenic 30.0%
TTCACCTATTAAACATATGG+AGG - chr1.2:72240826-72240845 MS.gene60804:intron 30.0%
! ATTCCACCAACTTTTGTTAA+GGG - chr1.2:72241424-72241443 MS.gene60804:intron 30.0%
! GACTTTGTTTGCATAATTGT+AGG - chr1.2:72241625-72241644 MS.gene60804:CDS 30.0%
! TATTCCACCAACTTTTGTTA+AGG - chr1.2:72241423-72241442 MS.gene60804:intron 30.0%
! TCTATCCAGGATGAATTTTA+AGG - chr1.2:72240996-72241015 MS.gene60804:CDS 30.0%
! TGGTGCATTTTCAATAACTT+GGG + chr1.2:72241289-72241308 None:intergenic 30.0%
AAAATCAAGGGATAGTGACT+TGG - chr1.2:72240783-72240802 MS.gene60804:intron 35.0%
AAATTCCTACATTACCAAGG+AGG - chr1.2:72240310-72240329 MS.gene60804:CDS 35.0%
AATCCCTTAACAAAAGTTGG+TGG + chr1.2:72241430-72241449 None:intergenic 35.0%
ACTAATTTGGTTATCCTCCT+TGG + chr1.2:72240327-72240346 None:intergenic 35.0%
ATCCTGCATTGTTACAAGTT+TGG - chr1.2:72240726-72240745 MS.gene60804:intron 35.0%
CTCAATCCCTTAACAAAAGT+TGG + chr1.2:72241433-72241452 None:intergenic 35.0%
TACCAAACTTGTAACAATGC+AGG + chr1.2:72240731-72240750 None:intergenic 35.0%
TGCTGGATAAACAAGATTAG+TGG + chr1.2:72240402-72240421 None:intergenic 35.0%
! CACAAAGCTTTATCACCAAA+TGG - chr1.2:72241701-72241720 MS.gene60804:CDS 35.0%
! CTAAGCTTTAGTTTCTATCC+AGG - chr1.2:72240983-72241002 MS.gene60804:CDS 35.0%
! GGTGCATTTTCAATAACTTG+GGG + chr1.2:72241288-72241307 None:intergenic 35.0%
! GTGGTGCATTTTCAATAACT+TGG + chr1.2:72241290-72241309 None:intergenic 35.0%
! TCAACATTAGTTGATGTTGG+AGG - chr1.2:72241192-72241211 MS.gene60804:CDS 35.0%
!! CAGGATGAATTTTAAGGAAG+CGG - chr1.2:72241002-72241021 MS.gene60804:CDS 35.0%
ACGGCAAGCAACTTGAAATT+TGG + chr1.2:72241885-72241904 None:intergenic 40.0%
ACTACTCAAACCATTGAGGA+TGG - chr1.2:72240605-72240624 MS.gene60804:intron 40.0%
ATAGCAGCATTTAACACTGC+TGG + chr1.2:72240419-72240438 None:intergenic 40.0%
ATCCTCAATGGTTTGAGTAG+TGG + chr1.2:72240606-72240625 None:intergenic 40.0%
ATGTTTGCAAGTGTTCCACA+GGG - chr1.2:72241468-72241487 MS.gene60804:intron 40.0%
CATGTTATACCTGAAAGTGG+TGG + chr1.2:72241309-72241328 None:intergenic 40.0%
CGCTTCCTTAAAATTCATCC+TGG + chr1.2:72241004-72241023 None:intergenic 40.0%
CTCATGTTTATCACAGTTGG+TGG - chr1.2:72241806-72241825 MS.gene60804:CDS 40.0%
GAGAAATTGAGCAAACTCTC+TGG - chr1.2:72241854-72241873 MS.gene60804:CDS 40.0%
GGGAAATTCCTACATTACCA+AGG - chr1.2:72240307-72240326 MS.gene60804:CDS 40.0%
TATGTTTGCAAGTGTTCCAC+AGG - chr1.2:72241467-72241486 MS.gene60804:intron 40.0%
TGTAGGCTGTATGCCATAAT+TGG - chr1.2:72241642-72241661 MS.gene60804:CDS 40.0%
TGTTAAGGGATTGAGCATGT+TGG - chr1.2:72241438-72241457 MS.gene60804:intron 40.0%
TTCCACTACTCAAACCATTG+AGG - chr1.2:72240601-72240620 MS.gene60804:intron 40.0%
TTTCAACAGTTTGGGAGTGA+TGG - chr1.2:72241907-72241926 MS.gene60804:CDS 40.0%
! ACATTAGTTGATGTTGGAGG+TGG - chr1.2:72241195-72241214 MS.gene60804:CDS 40.0%
! ACTGTCTTCAGTTTGTTCTG+AGG + chr1.2:72240354-72240373 None:intergenic 40.0%
! GACTTGTTCAAGAACGTTCA+TGG - chr1.2:72241042-72241061 MS.gene60804:CDS 40.0%
!! GATGAATTTTAAGGAAGCGG+TGG - chr1.2:72241005-72241024 MS.gene60804:CDS 40.0%
!! GTTATCCTCCTTGGTAATGT+AGG + chr1.2:72240318-72240337 None:intergenic 40.0%
!! GTTCTGAGGTAGCACTAATT+TGG + chr1.2:72240340-72240359 None:intergenic 40.0%
!! GTTTATCACAGTTGGTGGAA+GGG - chr1.2:72241811-72241830 MS.gene60804:CDS 40.0%
!! TCACTTGTGTTTGGTTCTTC+TGG + chr1.2:72241755-72241774 None:intergenic 40.0%
!! TGTTTATCACAGTTGGTGGA+AGG - chr1.2:72241810-72241829 MS.gene60804:CDS 40.0%
ACTCAAACCATTGAGGATGG+TGG - chr1.2:72240608-72240627 MS.gene60804:intron 45.0%
ACTTGCCTAATAGGCTTGAC+CGG - chr1.2:72240543-72240562 MS.gene60804:CDS 45.0%
CAGTGCATGTCTTTCAGCAA+TGG - chr1.2:72240370-72240389 MS.gene60804:CDS 45.0%
CTCCCAAACTGTTGAAAGCA+CGG + chr1.2:72241904-72241923 None:intergenic 45.0%
CTCTACTTTCATCAGGGACA+AGG + chr1.2:72240673-72240692 None:intergenic 45.0%
CTTGTCCCTGATGAAAGTAG+AGG - chr1.2:72240671-72240690 MS.gene60804:intron 45.0%
GAAAATGCACCACCACTTTC+AGG - chr1.2:72241297-72241316 MS.gene60804:intron 45.0%
GAGAGTTTACGGACTCTCAA+TGG - chr1.2:72240637-72240656 MS.gene60804:intron 45.0%
GTTTACGGACTCTCAATGGT+CGG - chr1.2:72240641-72240660 MS.gene60804:intron 45.0%
TAAGGGATTGAGCATGTTGG+AGG - chr1.2:72241441-72241460 MS.gene60804:intron 45.0%
TGAGAGTCCGTAAACTCTCT+CGG + chr1.2:72240636-72240655 None:intergenic 45.0%
TGCCGTGCTTTCAACAGTTT+GGG - chr1.2:72241899-72241918 MS.gene60804:CDS 45.0%
TTGCCGTGCTTTCAACAGTT+TGG - chr1.2:72241898-72241917 MS.gene60804:CDS 45.0%
!! GAATTTTAAGGAAGCGGTGG+TGG - chr1.2:72241008-72241027 MS.gene60804:CDS 45.0%
AGCACTCGGACTTGCCTAAT+AGG - chr1.2:72240534-72240553 MS.gene60804:CDS 50.0%
AGCATCCGGTCAAGCCTATT+AGG + chr1.2:72240551-72240570 None:intergenic 50.0%
AGTATCATGGCATCACCCTG+TGG + chr1.2:72241486-72241505 None:intergenic 50.0%
ATAGCTAAGGCAACACCACC+TGG - chr1.2:72240461-72240480 MS.gene60804:CDS 50.0%
GGGTGATGCCATGATACTGA+AGG - chr1.2:72241488-72241507 MS.gene60804:intron 50.0%
GTTGATGTTGGAGGTGGAAG+TGG - chr1.2:72241201-72241220 MS.gene60804:CDS 50.0%
TAGCAAGCAAACGCAGCATC+CGG + chr1.2:72240565-72240584 None:intergenic 50.0%
!! GATGGTGACATGAAAGCACC+AGG + chr1.2:72240482-72240501 None:intergenic 50.0%
ACCAGCATCAACGCAGCACT+CGG - chr1.2:72240520-72240539 MS.gene60804:CDS 55.0%
! GGTGGTGCCGAGAGAGTTTA+CGG - chr1.2:72240626-72240645 MS.gene60804:intron 55.0%
!! GGTGACATGAAAGCACCAGG+TGG + chr1.2:72240479-72240498 None:intergenic 55.0%
CTCGGCACCACCATCCTCAA+TGG + chr1.2:72240618-72240637 None:intergenic 60.0%
! TCCGAGTGCTGCGTTGATGC+TGG + chr1.2:72240524-72240543 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 72240305 72241943 72240305 ID=MS.gene60804
chr1.2 mRNA 72240305 72241943 72240305 ID=MS.gene60804.t1;Parent=MS.gene60804
chr1.2 exon 72241501 72241943 72241501 ID=MS.gene60804.t1.exon1;Parent=MS.gene60804.t1
chr1.2 CDS 72241501 72241943 72241501 ID=cds.MS.gene60804.t1;Parent=MS.gene60804.t1
chr1.2 exon 72240930 72241243 72240930 ID=MS.gene60804.t1.exon2;Parent=MS.gene60804.t1
chr1.2 CDS 72240930 72241243 72240930 ID=cds.MS.gene60804.t1;Parent=MS.gene60804.t1
chr1.2 exon 72240305 72240579 72240305 ID=MS.gene60804.t1.exon3;Parent=MS.gene60804.t1
chr1.2 CDS 72240305 72240579 72240305 ID=cds.MS.gene60804.t1;Parent=MS.gene60804.t1
Gene Sequence

>MS.gene60804

ATGGGAAATTCCTACATTACCAAGGAGGATAACCAAATTAGTGCTACCTCAGAACAAACTGAAGACAGTGCATGTCTTTCAGCAATGGTACTTACCACTAATCTTGTTTATCCAGCAGTGTTAAATGCTGCTATTGATCTCAATTTATTTGAGATCATAGCTAAGGCAACACCACCTGGTGCTTTCATGTCACCATCAGAAATTGCTTCTAAATTACCAGCATCAACGCAGCACTCGGACTTGCCTAATAGGCTTGACCGGATGCTGCGTTTGCTTGCTAGTTATTCTGTTCTTACTTCCACTACTCAAACCATTGAGGATGGTGGTGCCGAGAGAGTTTACGGACTCTCAATGGTCGGAAAATACCTTGTCCCTGATGAAAGTAGAGGTTATTTGGCTTCATTTACTACATTTCTATGTTATCCTGCATTGTTACAAGTTTGGTAAGTTATTTATTTCTCATTTCTCATACATATATAAAATCAAGGGATAGTGACTTGGATAAATAGTTTAATTAAATATTCACCTATTAAACATATGGAGGATATATAAGTTATAAAGGTAGCTTTATAAAATAAAAAAAAAAAAAGTTAATATAAATAATGTTAAAGAGAAAGTGATAGTTTAAGCTAATTGTAGCAATCAGGTATTTAATCTTACTTTTGTTCCAATTATGGTCTAAGCTTTAGTTTCTATCCAGGATGAATTTTAAGGAAGCGGTGGTGGATGAAGACATTGACTTGTTCAAGAACGTTCATGGAGTGACAAAGTATGAATTCATGGGAAAGGATAAAAAAATGAACCAAATTTTTAACAAATCAATGGTTGATGTATGTGCTACAGAGATGAAAAGAATGCTTGAAATATACACTGGATTTGAGGGAATATCAACATTAGTTGATGTTGGAGGTGGAAGTGGAAGAAATCTTGAATTGATAATATCCAAATATCCATTAATAAAGGGAATTAACTTTGATCTTCCCCAAGTTATTGAAAATGCACCACCACTTTCAGGTATAACATGATATATAATAATTTTTCTTCACATATATCATTATCAACATGCCATCTTATACTAAAGTTTTACTTTGCATGTATATTATAATTAATAAAGTATATATTCCACCAACTTTTGTTAAGGGATTGAGCATGTTGGAGGAGATATGTTTGCAAGTGTTCCACAGGGTGATGCCATGATACTGAAGGTAAGTATATAAATATTAATTAGTTAGATCTAACTGATTAATTAATTACTTGAAACTTTATATAGATTAATTTATCTGATTTTTTTAATTAAAGTGCTAACTACAACTTTTTGTTGACTTTGTTTGCATAATTGTAGGCTGTATGCCATAATTGGTCAGATGAAAAATGCATAGAATTTTTAAGCAATTGTCACAAAGCTTTATCACCAAATGGAAAAGTGATTATTGTGGAGTTCATATTGCCAGAAGAACCAAACACAAGTGAAGAATCTAAGCTTGTTTCAACTCTTGACAATCTCATGTTTATCACAGTTGGTGGAAGGGAAAGAACTGAGAAACAATATGAGAAATTGAGCAAACTCTCTGGATTTTCCAAATTTCAAGTTGCTTGCCGTGCTTTCAACAGTTTGGGAGTGATGGAATTTTATAAATGA
Protein sequence

>MS.gene60804.t1

MGNSYITKEDNQISATSEQTEDSACLSAMVLTTNLVYPAVLNAAIDLNLFEIIAKATPPGAFMSPSEIASKLPASTQHSDLPNRLDRMLRLLASYSVLTSTTQTIEDGGAERVYGLSMVGKYLVPDESRGYLASFTTFLCYPALLQVWMNFKEAVVDEDIDLFKNVHGVTKYEFMGKDKKMNQIFNKSMVDVCATEMKRMLEIYTGFEGISTLVDVGGGSGRNLELIISKYPLIKGINFDLPQVIENAPPLSDEKCIEFLSNCHKALSPNGKVIIVEFILPEEPNTSEESKLVSTLDNLMFITVGGRERTEKQYEKLSKLSGFSKFQVACRAFNSLGVMEFYK