Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61050.t1 | XP_004509954.1 | 96.5 | 113 | 4 | 0 | 36 | 148 | 249 | 361 | 1.40E-58 | 235.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61050.t1 | Q9ZPQ3 | 69.6 | 115 | 31 | 1 | 37 | 147 | 202 | 316 | 3.6e-44 | 179.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61050.t1 | A0A1S2YU38 | 96.5 | 113 | 4 | 0 | 36 | 148 | 249 | 361 | 9.8e-59 | 235.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene61050 | 0.817962 | 2.51E-52 | -1.69E-46 |
| MS.gene050026 | MS.gene61050 | 0.800549 | 1.37E-48 | -1.69E-46 |
| MS.gene050355 | MS.gene61050 | 0.805981 | 1.03E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene61050 | 0.816903 | 4.35E-52 | -1.69E-46 |
| MS.gene052867 | MS.gene61050 | 0.809056 | 2.28E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene61050 | 0.814714 | 1.34E-51 | -1.69E-46 |
| MS.gene054236 | MS.gene61050 | 0.815826 | 7.57E-52 | -1.69E-46 |
| MS.gene055586 | MS.gene61050 | 0.813371 | 2.65E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene61050 | 0.819201 | 1.32E-52 | -1.69E-46 |
| MS.gene057026 | MS.gene61050 | 0.818049 | 2.40E-52 | -1.69E-46 |
| MS.gene058037 | MS.gene61050 | 0.81166 | 6.26E-51 | -1.69E-46 |
| MS.gene058979 | MS.gene61050 | 0.818215 | 2.20E-52 | -1.69E-46 |
| MS.gene060572 | MS.gene61050 | 0.811472 | 6.88E-51 | -1.69E-46 |
| MS.gene061040 | MS.gene61050 | -0.813599 | 2.36E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61050.t1 | MTR_8g066120 | 95.575 | 113 | 5 | 0 | 36 | 148 | 201 | 313 | 2.20e-75 | 227 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61050.t1 | AT2G03800 | 69.565 | 115 | 31 | 1 | 37 | 147 | 202 | 316 | 1.30e-54 | 174 |
| MS.gene61050.t1 | AT2G03800 | 69.565 | 115 | 31 | 1 | 37 | 147 | 246 | 360 | 2.45e-54 | 175 |
Find 36 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTACATTTGTTTCTCCTTT+TGG | 0.150136 | 8.1:-38355887 | None:intergenic |
| TGGGTTGGTCACCTACTTTC+TGG | 0.174476 | 8.1:+38355842 | MS.gene61050:CDS |
| AACACTCAATAAAAGTAATT+TGG | 0.197331 | 8.1:-38356310 | None:intergenic |
| ACGGAGCAGAATATTAAAAT+TGG | 0.260579 | 8.1:+38356283 | MS.gene61050:CDS |
| TCAATGCAGAGCCAATATTT+AGG | 0.285922 | 8.1:+38355282 | MS.gene61050:CDS |
| AATATAGGAAAGATGATGTT+TGG | 0.316445 | 8.1:+38355822 | MS.gene61050:intron |
| TCACCTACTTTCTGGATATT+CGG | 0.318220 | 8.1:+38355850 | MS.gene61050:CDS |
| AATGAACCAAACCTAAATAT+TGG | 0.350210 | 8.1:-38355293 | None:intergenic |
| GATAAGAAGAAAGTCCTTCT+TGG | 0.358937 | 8.1:+38355398 | MS.gene61050:CDS |
| GGAGAAACAAATGTAGAGAT+TGG | 0.379058 | 8.1:+38355893 | MS.gene61050:CDS |
| ACATTATGTGCCTCGGCATA+TGG | 0.407678 | 8.1:+38355433 | MS.gene61050:CDS |
| AATTTACTGCAGGAGTTTCA+AGG | 0.417491 | 8.1:+38356228 | MS.gene61050:intron |
| AGAAGGCCTCAATGCTCAAA+AGG | 0.427209 | 8.1:-38355202 | None:intergenic |
| GTCTATAAAAGCTGCATATG+AGG | 0.434564 | 8.1:+38355931 | MS.gene61050:CDS |
| ATAAGAAGAAAGTCCTTCTT+GGG | 0.451631 | 8.1:+38355399 | MS.gene61050:CDS |
| TTCTGGATATTCGGTGCCAA+TGG | 0.497093 | 8.1:+38355859 | MS.gene61050:CDS |
| ATGTAGAGATTGGAGGAACT+TGG | 0.504571 | 8.1:+38355903 | MS.gene61050:CDS |
| ATATAGGAAAGATGATGTTT+GGG | 0.508580 | 8.1:+38355823 | MS.gene61050:intron |
| GTCCTTCTTGGGATAGGCGG+TGG | 0.509514 | 8.1:+38355410 | MS.gene61050:CDS |
| ATTTACTGCAGGAGTTTCAA+GGG | 0.511764 | 8.1:+38356229 | MS.gene61050:intron |
| TGGCAGAAGAATGCCATCAC+AGG | 0.525499 | 8.1:+38356253 | MS.gene61050:CDS |
| TGCGCAATAATTTCGCCACC+AGG | 0.526598 | 8.1:-38355971 | None:intergenic |
| AGGAAAGATGATGTTTGGGT+TGG | 0.533495 | 8.1:+38355827 | MS.gene61050:intron |
| AAGAAAGTCCTTCTTGGGAT+AGG | 0.543921 | 8.1:+38355404 | MS.gene61050:CDS |
| ACTGCAGGAGTTTCAAGGGC+TGG | 0.545446 | 8.1:+38356233 | MS.gene61050:intron |
| GAAACAAATGTAGAGATTGG+AGG | 0.558298 | 8.1:+38355896 | MS.gene61050:CDS |
| GAAGGCCTCAATGCTCAAAA+GGG | 0.561268 | 8.1:-38355201 | None:intergenic |
| CTCCGTGAGGAATCCTGTGA+TGG | 0.563242 | 8.1:-38356266 | None:intergenic |
| GCACCGAATATCCAGAAAGT+AGG | 0.580008 | 8.1:-38355853 | None:intergenic |
| GTGCCAATGGAAGATCCAAA+AGG | 0.584393 | 8.1:+38355872 | MS.gene61050:CDS |
| GCGGTGGACATTATGTGCCT+CGG | 0.594821 | 8.1:+38355426 | MS.gene61050:CDS |
| TTATTGCGCATCTTGACCAA+AGG | 0.621535 | 8.1:+38355984 | MS.gene61050:CDS |
| GTCCACCGCCTATCCCAAGA+AGG | 0.624058 | 8.1:-38355412 | None:intergenic |
| TGCCATCACAGGATTCCTCA+CGG | 0.636856 | 8.1:+38356264 | MS.gene61050:CDS |
| AAAGTCCTTCTTGGGATAGG+CGG | 0.642444 | 8.1:+38355407 | MS.gene61050:CDS |
| AACACTACATCCATATGCCG+AGG | 0.663300 | 8.1:-38355443 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGTTAATTTTTTTTATTCA+AGG | - | chr8.1:38355784-38355803 | None:intergenic | 10.0% |
| !!! | GTTTGATATATATATTTTTT+TGG | + | chr8.1:38356131-38356150 | MS.gene61050:intron | 10.0% |
| !! | CATTGATAAAAAATTTAACT+TGG | - | chr8.1:38355268-38355287 | None:intergenic | 15.0% |
| !! | ATTATGGGAATAAATTACAT+TGG | + | chr8.1:38355543-38355562 | MS.gene61050:intron | 20.0% |
| !! | TTCTATAATGAAAAATGAGA+AGG | - | chr8.1:38355222-38355241 | None:intergenic | 20.0% |
| !!! | TTAAAGTTTCCTTCTAAAAT+CGG | + | chr8.1:38355323-38355342 | MS.gene61050:intron | 20.0% |
| !!! | TTTTTTTATTCAAGGTAACA+AGG | - | chr8.1:38355776-38355795 | None:intergenic | 20.0% |
| ! | AATATAGGAAAGATGATGTT+TGG | + | chr8.1:38355822-38355841 | MS.gene61050:intron | 25.0% |
| ! | AATGAACCAAACCTAAATAT+TGG | - | chr8.1:38355296-38355315 | None:intergenic | 25.0% |
| ! | AATTACATTGGTGAAAAACA+TGG | + | chr8.1:38355555-38355574 | MS.gene61050:intron | 25.0% |
| ! | ATATAGGAAAGATGATGTTT+GGG | + | chr8.1:38355823-38355842 | MS.gene61050:intron | 25.0% |
| ! | ATTACATTGGTGAAAAACAT+GGG | + | chr8.1:38355556-38355575 | MS.gene61050:intron | 25.0% |
| ! | CAATTATAAAGTCACTGAAA+TGG | - | chr8.1:38355477-38355496 | None:intergenic | 25.0% |
| ! | TGCACACTTAAATAGATTAT+GGG | + | chr8.1:38355528-38355547 | MS.gene61050:intron | 25.0% |
| ! | TTAGAACTCGAGTAAAAAAA+AGG | - | chr8.1:38355694-38355713 | None:intergenic | 25.0% |
| !! | GAATTTTCTGATTGATGAAA+GGG | + | chr8.1:38355496-38355515 | MS.gene61050:intron | 25.0% |
| !! | TACGATTTGAAACTGATAAT+AGG | + | chr8.1:38356162-38356181 | MS.gene61050:intron | 25.0% |
| !! | TGAATTTTCTGATTGATGAA+AGG | + | chr8.1:38355495-38355514 | MS.gene61050:intron | 25.0% |
| !!! | AAATTTTCACCGATTTTAGA+AGG | - | chr8.1:38355335-38355354 | None:intergenic | 25.0% |
| !!! | TTTACTCTAAAATTTACTGC+AGG | + | chr8.1:38356218-38356237 | MS.gene61050:intron | 25.0% |
| ACGGAGCAGAATATTAAAAT+TGG | + | chr8.1:38356283-38356302 | MS.gene61050:CDS | 30.0% | |
| ATAAGAAGAAAGTCCTTCTT+GGG | + | chr8.1:38355399-38355418 | MS.gene61050:CDS | 30.0% | |
| ATGCACATTAAACTTACCTT+TGG | - | chr8.1:38356003-38356022 | None:intergenic | 30.0% | |
| CTGCACACTTAAATAGATTA+TGG | + | chr8.1:38355527-38355546 | MS.gene61050:intron | 30.0% | |
| TGCAAACAATTGATTACAGA+AGG | - | chr8.1:38356029-38356048 | None:intergenic | 30.0% | |
| !! | TCTACATTTGTTTCTCCTTT+TGG | - | chr8.1:38355890-38355909 | None:intergenic | 30.0% |
| !!! | TGCTTTGCTTTTTGCAATAT+AGG | + | chr8.1:38355807-38355826 | MS.gene61050:intron | 30.0% |
| AATTTACTGCAGGAGTTTCA+AGG | + | chr8.1:38356228-38356247 | MS.gene61050:intron | 35.0% | |
| ATTTACTGCAGGAGTTTCAA+GGG | + | chr8.1:38356229-38356248 | MS.gene61050:intron | 35.0% | |
| CACCTTTATCTTCATTCCAA+TGG | - | chr8.1:38355591-38355610 | None:intergenic | 35.0% | |
| CATTGGAATGAAGATAAAGG+TGG | + | chr8.1:38355589-38355608 | MS.gene61050:intron | 35.0% | |
| CTCCATTGGAATGAAGATAA+AGG | + | chr8.1:38355586-38355605 | MS.gene61050:intron | 35.0% | |
| GAAACAAATGTAGAGATTGG+AGG | + | chr8.1:38355896-38355915 | MS.gene61050:CDS | 35.0% | |
| GATAAGAAGAAAGTCCTTCT+TGG | + | chr8.1:38355398-38355417 | MS.gene61050:CDS | 35.0% | |
| GGAGAAACAAATGTAGAGAT+TGG | + | chr8.1:38355893-38355912 | MS.gene61050:CDS | 35.0% | |
| GTCTATAAAAGCTGCATATG+AGG | + | chr8.1:38355931-38355950 | MS.gene61050:CDS | 35.0% | |
| TCAATGCAGAGCCAATATTT+AGG | + | chr8.1:38355282-38355301 | MS.gene61050:CDS | 35.0% | |
| TCACCTACTTTCTGGATATT+CGG | + | chr8.1:38355850-38355869 | MS.gene61050:CDS | 35.0% | |
| ! | TTTTAATATTCTGCTCCGTG+AGG | - | chr8.1:38356282-38356301 | None:intergenic | 35.0% |
| AAGAAAGTCCTTCTTGGGAT+AGG | + | chr8.1:38355404-38355423 | MS.gene61050:CDS | 40.0% | |
| ACATGGGAGTTAAACTCCAT+TGG | + | chr8.1:38355572-38355591 | MS.gene61050:intron | 40.0% | |
| AGGAAAGATGATGTTTGGGT+TGG | + | chr8.1:38355827-38355846 | MS.gene61050:intron | 40.0% | |
| ATGTAGAGATTGGAGGAACT+TGG | + | chr8.1:38355903-38355922 | MS.gene61050:CDS | 40.0% | |
| TTATTGCGCATCTTGACCAA+AGG | + | chr8.1:38355984-38356003 | MS.gene61050:CDS | 40.0% | |
| ! | GCAGAGCCAATATTTAGGTT+TGG | + | chr8.1:38355287-38355306 | MS.gene61050:intron | 40.0% |
| ! | TCTCCTTTTGGATCTTCCAT+TGG | - | chr8.1:38355878-38355897 | None:intergenic | 40.0% |
| !! | ACTAAATCTGCTTTTCCTGG+TGG | + | chr8.1:38355956-38355975 | MS.gene61050:CDS | 40.0% |
| !! | GCAACTAAATCTGCTTTTCC+TGG | + | chr8.1:38355953-38355972 | MS.gene61050:CDS | 40.0% |
| AAAGTCCTTCTTGGGATAGG+CGG | + | chr8.1:38355407-38355426 | MS.gene61050:CDS | 45.0% | |
| AACACTACATCCATATGCCG+AGG | - | chr8.1:38355446-38355465 | None:intergenic | 45.0% | |
| ACATTATGTGCCTCGGCATA+TGG | + | chr8.1:38355433-38355452 | MS.gene61050:CDS | 45.0% | |
| AGAAGGCCTCAATGCTCAAA+AGG | - | chr8.1:38355205-38355224 | None:intergenic | 45.0% | |
| ATTCTCACACTCTTCCTGCA+GGG | + | chr8.1:38355370-38355389 | MS.gene61050:intron | 45.0% | |
| CTTCTTATCATTCTCCCTGC+AGG | - | chr8.1:38355387-38355406 | None:intergenic | 45.0% | |
| GAAGGCCTCAATGCTCAAAA+GGG | - | chr8.1:38355204-38355223 | None:intergenic | 45.0% | |
| GCACCGAATATCCAGAAAGT+AGG | - | chr8.1:38355856-38355875 | None:intergenic | 45.0% | |
| GTGCCAATGGAAGATCCAAA+AGG | + | chr8.1:38355872-38355891 | MS.gene61050:CDS | 45.0% | |
| !! | TTCTGGATATTCGGTGCCAA+TGG | + | chr8.1:38355859-38355878 | MS.gene61050:CDS | 45.0% |
| !!! | TTTGATATATATATTTTTTT+GGG | + | chr8.1:38356132-38356151 | MS.gene61050:intron | 5.0% |
| GATTCTCACACTCTTCCTGC+AGG | + | chr8.1:38355369-38355388 | MS.gene61050:intron | 50.0% | |
| TGCCATCACAGGATTCCTCA+CGG | + | chr8.1:38356264-38356283 | MS.gene61050:CDS | 50.0% | |
| TGCGCAATAATTTCGCCACC+AGG | - | chr8.1:38355974-38355993 | None:intergenic | 50.0% | |
| TGGCAGAAGAATGCCATCAC+AGG | + | chr8.1:38356253-38356272 | MS.gene61050:CDS | 50.0% | |
| !! | TGGGTTGGTCACCTACTTTC+TGG | + | chr8.1:38355842-38355861 | MS.gene61050:CDS | 50.0% |
| ACTGCAGGAGTTTCAAGGGC+TGG | + | chr8.1:38356233-38356252 | MS.gene61050:intron | 55.0% | |
| CTCCGTGAGGAATCCTGTGA+TGG | - | chr8.1:38356269-38356288 | None:intergenic | 55.0% | |
| GCGGTGGACATTATGTGCCT+CGG | + | chr8.1:38355426-38355445 | MS.gene61050:CDS | 55.0% | |
| GTCCACCGCCTATCCCAAGA+AGG | - | chr8.1:38355415-38355434 | None:intergenic | 60.0% | |
| GTCCTTCTTGGGATAGGCGG+TGG | + | chr8.1:38355410-38355429 | MS.gene61050:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 38355197 | 38356327 | 38355197 | ID=MS.gene61050 |
| chr8.1 | mRNA | 38355197 | 38356327 | 38355197 | ID=MS.gene61050.t1;Parent=MS.gene61050 |
| chr8.1 | exon | 38355197 | 38355303 | 38355197 | ID=MS.gene61050.t1.exon1;Parent=MS.gene61050.t1 |
| chr8.1 | CDS | 38355197 | 38355303 | 38355197 | ID=cds.MS.gene61050.t1;Parent=MS.gene61050.t1 |
| chr8.1 | exon | 38355391 | 38355465 | 38355391 | ID=MS.gene61050.t1.exon2;Parent=MS.gene61050.t1 |
| chr8.1 | CDS | 38355391 | 38355465 | 38355391 | ID=cds.MS.gene61050.t1;Parent=MS.gene61050.t1 |
| chr8.1 | exon | 38355829 | 38356005 | 38355829 | ID=MS.gene61050.t1.exon3;Parent=MS.gene61050.t1 |
| chr8.1 | CDS | 38355829 | 38356005 | 38355829 | ID=cds.MS.gene61050.t1;Parent=MS.gene61050.t1 |
| chr8.1 | exon | 38356240 | 38356327 | 38356240 | ID=MS.gene61050.t1.exon4;Parent=MS.gene61050.t1 |
| chr8.1 | CDS | 38356240 | 38356327 | 38356240 | ID=cds.MS.gene61050.t1;Parent=MS.gene61050.t1 |
| Gene Sequence |
| Protein sequence |
