AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene61920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61920.t1 MTR_5g068000 59.286 140 41 2 3 126 58 197 5.55e-50 159
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 34 sgRNAs with CRISPR-Local

Find 258 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACGCTCAGGTCAACCTTTA+AGG 0.165641 5.2:-57731337 MS.gene61920:CDS
CCATCTTCCACAACCAATTA+AGG 0.188995 5.2:-57729062 MS.gene61920:CDS
AAATATACCTGCATCCTTAA+AGG 0.205975 5.2:+57731323 None:intergenic
TCGGGATTTGGAACATAAAT+TGG 0.221035 5.2:+57732323 None:intergenic
ATTTCTCTCACTTCGGGATT+TGG 0.228092 5.2:+57732311 None:intergenic
TCCATCATTTCTCTCACTTC+GGG 0.319954 5.2:+57732305 None:intergenic
GGTTTGTTAGGACCCTTAAT+TGG 0.349887 5.2:+57729049 None:intergenic
TGGGGTAACACAGGTTTGTT+AGG 0.363172 5.2:+57729037 None:intergenic
AACACTGCTGAGATTCAAAT+TGG 0.390939 5.2:-57732392 MS.gene61920:CDS
CCTTAATTGGTTGTGGAAGA+TGG 0.399501 5.2:+57729062 None:intergenic
TATAATAGGTCCAACTTGCT+TGG 0.414650 5.2:-57731367 MS.gene61920:intron
CTCCATCATTTCTCTCACTT+CGG 0.419780 5.2:+57732304 None:intergenic
GGCTGCAACGCCTAACCAAC+CGG 0.421388 5.2:-57728716 MS.gene61920:CDS
TACATGTCAGGTTTATATGT+TGG 0.434809 5.2:-57728459 MS.gene61920:intron
AGAATCTTGTCGCATTTGTC+TGG 0.448016 5.2:-57728613 MS.gene61920:intron
GAGAGAAATGATGGAGCATA+AGG 0.453917 5.2:-57732297 MS.gene61920:intron
TTAGGACCCTTAATTGGTTG+TGG 0.477232 5.2:+57729055 None:intergenic
ATAATAGGTCCAACTTGCTT+GGG 0.487519 5.2:-57731366 MS.gene61920:intron
CATCTTCCACAACCAATTAA+GGG 0.500403 5.2:-57729061 MS.gene61920:CDS
TCCCGAAGTGAGAGAAATGA+TGG 0.511936 5.2:-57732306 MS.gene61920:CDS
CTTCCACTCTGCGTATCCAC+CGG 0.514658 5.2:+57728697 None:intergenic
GCTTGGGAACGCGAACGCTC+AGG 0.517258 5.2:-57731350 MS.gene61920:CDS
AGGTCAACCTTTAAGGATGC+AGG 0.519968 5.2:-57731330 MS.gene61920:intron
AGTTGAAAGGTTTGAGCTTG+TGG 0.524673 5.2:-57728655 MS.gene61920:CDS
GTCGAACAAGTAACAGTTGG+TGG 0.535790 5.2:-57732362 MS.gene61920:CDS
CACTCTGCGTATCCACCGGT+TGG 0.540807 5.2:+57728701 None:intergenic
TGCGTATCCACCGGTTGGTT+AGG 0.553675 5.2:+57728706 None:intergenic
GTTAGGCGTTGCAGCCACGC+CGG 0.563359 5.2:+57728723 None:intergenic
ATAATAATTTGCAGCCGGCG+TGG 0.573736 5.2:-57728737 MS.gene61920:intron
GTTCGCGTTCCCAAGCAAGT+TGG 0.577353 5.2:+57731357 None:intergenic
TGACAGATATAGCAGTTGAA+AGG 0.598292 5.2:-57728668 MS.gene61920:CDS
AATGTCGAACAAGTAACAGT+TGG 0.644511 5.2:-57732365 MS.gene61920:CDS
CAACCGGTGGATACGCAGAG+TGG 0.687210 5.2:-57728700 MS.gene61920:CDS
TGCAACGCCTAACCAACCGG+TGG 0.704756 5.2:-57728713 MS.gene61920:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAATATATAAATATTAA+AGG + chr5.2:57728755-57728774 None:intergenic 0.0%
!! AATATATAAATATTAAAGGA+AGG + chr5.2:57728751-57728770 None:intergenic 10.0%
!! TTAAAATGAAAAAAAGATAA+AGG - chr5.2:57731562-57731581 MS.gene61920:intron 10.0%
!! TTAAAATGAAAAAAAGATAA+AGG + chr5.2:57731581-57731562 None:intergenic 10.0%
!! TTATACAAGAATTTATAATT+AGG - chr5.2:57730140-57730159 MS.gene61920:intron 10.0%
!!! ATCTTTTTTTCATTTTAATT+TGG - chr5.2:57731390-57731409 MS.gene61920:intron 10.0%
!!! ATCTTTTTTTCATTTTAATT+TGG + chr5.2:57731409-57731390 None:intergenic 10.0%
!! CTTAAAAATTCATATGAAAA+TGG + chr5.2:57731426-57731445 None:intergenic 15.0%
!! TAAGTGAAATTCTATATTTA+AGG - chr5.2:57729112-57729131 MS.gene61920:intron 15.0%
!!! TATTTATTTAGAATCCAATA+AGG - chr5.2:57728587-57728606 MS.gene61920:intron 15.0%
!!! TCAATTTTTTTCTTTTATGA+GGG - chr5.2:57728825-57728844 MS.gene61920:intron 15.0%
!!! TCATTTTAAATATTAGTTCA+CGG + chr5.2:57729707-57729726 None:intergenic 15.0%
!!! TTCATGATTTTATTTTCTTA+TGG - chr5.2:57731453-57731472 MS.gene61920:intron 15.0%
!!! TTTTTTTTCTTCTTATAGTA+AGG - chr5.2:57728909-57728928 MS.gene61920:intron 15.0%
!! AAACTACTACTATTAATTTC+AGG + chr5.2:57731667-57731686 None:intergenic 20.0%
!! AATGATCTTATAGATTTGTA+AGG + chr5.2:57729603-57729622 None:intergenic 20.0%
!! ATTACCTGAAATTAAGTTAA+GGG - chr5.2:57731593-57731612 MS.gene61920:intron 20.0%
!! ATTACCTGAAATTAAGTTAA+GGG + chr5.2:57731612-57731593 None:intergenic 20.0%
!! ATTATACATGAAAACTAATG+AGG + chr5.2:57729462-57729481 None:intergenic 20.0%
!! TATTACCTGAAATTAAGTTA+AGG - chr5.2:57731592-57731611 MS.gene61920:intron 20.0%
!! TATTACCTGAAATTAAGTTA+AGG + chr5.2:57731611-57731592 None:intergenic 20.0%
!!! AATATTAATTTTAGTGTCTC+AGG + chr5.2:57729284-57729303 None:intergenic 20.0%
!!! AATCTAGATTTTCTTGATTT+AGG - chr5.2:57730728-57730747 MS.gene61920:intron 20.0%
!!! ATATTAATTTTAGTGTCTCA+GGG + chr5.2:57729283-57729302 None:intergenic 20.0%
!!! CATTAGTTTTCATGTATAAT+AGG - chr5.2:57729462-57729481 MS.gene61920:intron 20.0%
!!! GTCAATTTTTTTCTTTTATG+AGG - chr5.2:57728824-57728843 MS.gene61920:intron 20.0%
!!! TCATTTTTATTACCACATTT+GGG - chr5.2:57728988-57729007 MS.gene61920:intron 20.0%
!!! TTCATTTTTATTACCACATT+TGG - chr5.2:57728987-57729006 MS.gene61920:intron 20.0%
!!! TTGACTTTTGAGTTTATTTT+TGG - chr5.2:57729966-57729985 MS.gene61920:intron 20.0%
! AAAATTATGTTTCATCTGTG+TGG - chr5.2:57730763-57730782 MS.gene61920:intron 25.0%
! AGAAAACTTTCTTATATCGA+AGG + chr5.2:57728655-57728674 None:intergenic 25.0%
! AGAATGTAATACTATGAGAA+AGG + chr5.2:57731980-57731999 None:intergenic 25.0%
! ATAAAAAAGGAATTACAAGC+TGG + chr5.2:57731238-57731257 None:intergenic 25.0%
! ATCTTATAGATTTGTAAGGA+TGG + chr5.2:57729599-57729618 None:intergenic 25.0%
! GATCAGTGATGAATTAATTT+AGG - chr5.2:57731313-57731332 MS.gene61920:intron 25.0%
! GATCAGTGATGAATTAATTT+AGG + chr5.2:57731332-57731313 None:intergenic 25.0%
! TAAAAAAGGAATTACAAGCT+GGG + chr5.2:57731237-57731256 None:intergenic 25.0%
! TAAGCATTCAATGAAAAGTA+AGG + chr5.2:57732079-57732098 None:intergenic 25.0%
! TCATATGAAAATGGACAAAA+AGG + chr5.2:57731417-57731436 None:intergenic 25.0%
! TCTCAACTGCAAATTAATAT+AGG + chr5.2:57729399-57729418 None:intergenic 25.0%
! TCTTAGCTATCTTGATATTT+AGG - chr5.2:57729641-57729660 MS.gene61920:intron 25.0%
!! CATATTACTTGTAAGCTTTT+AGG - chr5.2:57730060-57730079 MS.gene61920:intron 25.0%
!! CTTAATTTCAGGTAATAGTT+TGG + chr5.2:57731380-57731361 None:intergenic 25.0%
!! TAATAGTAGTAGTTTTCTCT+AGG - chr5.2:57731672-57731691 MS.gene61920:intron 25.0%
!! TTAGATTTGAAGCTTGAAAA+TGG - chr5.2:57731524-57731543 MS.gene61920:intron 25.0%
!! TTTAAGATTAACATCAACCA+AGG + chr5.2:57730710-57730729 None:intergenic 25.0%
!!! GTTTGGAGTTTCAAATATTT+TGG - chr5.2:57729874-57729893 MS.gene61920:intron 25.0%
AAAAAAGGAATTACAAGCTG+GGG + chr5.2:57731236-57731255 None:intergenic 30.0%
AAATATACCTGCATCCTTAA+AGG + chr5.2:57729523-57729542 None:intergenic 30.0%
AACTTTCTTATATCGAAGGT+CGG + chr5.2:57728651-57728670 None:intergenic 30.0%
AAGCTTGAAAATGGAGAAAA+AGG - chr5.2:57731533-57731552 MS.gene61920:intron 30.0%
AAGGTTGAAAAAGGAAAGTT+TGG + chr5.2:57730338-57730357 None:intergenic 30.0%
ACACAAAAAAAGAGAGATTG+TGG + chr5.2:57731928-57731947 None:intergenic 30.0%
AGAGTTTGACTATGATTTAG+TGG - chr5.2:57729422-57729441 MS.gene61920:intron 30.0%
ATAATCTGATTGCTGAAGAT+GGG + chr5.2:57731828-57731847 None:intergenic 30.0%
ATTCTTAGTAAACATAGACG+AGG + chr5.2:57731503-57731522 None:intergenic 30.0%
CATATAAACCTGACATGTAA+AGG + chr5.2:57732383-57732402 None:intergenic 30.0%
CTTTATAAGATTCCTTGAGA+TGG - chr5.2:57729999-57730018 MS.gene61920:intron 30.0%
GAGTTTGACTATGATTTAGT+GGG - chr5.2:57729423-57729442 MS.gene61920:intron 30.0%
TACATGTCAGGTTTATATGT+TGG - chr5.2:57732384-57732403 MS.gene61920:CDS 30.0%
TAGTCCCTTAACTTAATTTC+AGG - chr5.2:57731350-57731369 MS.gene61920:CDS 30.0%
TAGTCCCTTAACTTAATTTC+AGG + chr5.2:57731369-57731350 None:intergenic 30.0%
TGATTCTATAAACCATCTCA+AGG + chr5.2:57730014-57730033 None:intergenic 30.0%
TTTCTCTAATTAGTGTCTCA+GGG - chr5.2:57729356-57729375 MS.gene61920:intron 30.0%
! AATGTTTAGAGTATCAAGGT+AGG + chr5.2:57731034-57731053 None:intergenic 30.0%
! ACGTTTTCAATATCATCTGA+GGG - chr5.2:57732265-57732284 MS.gene61920:intron 30.0%
! CATGAATGTTTAGAGTATCA+AGG + chr5.2:57731038-57731057 None:intergenic 30.0%
! TACGAATAATAATTTGCAGC+CGG - chr5.2:57732101-57732120 MS.gene61920:intron 30.0%
! TACGTTTTCAATATCATCTG+AGG - chr5.2:57732264-57732283 MS.gene61920:intron 30.0%
! TATTTAGGATGTCTGTTTGT+AGG - chr5.2:57729656-57729675 MS.gene61920:intron 30.0%
! TCACATGCATTTTACCTTAT+TGG + chr5.2:57728604-57728623 None:intergenic 30.0%
!! AAACGTGTTTTTACAGGATA+AGG - chr5.2:57731727-57731746 MS.gene61920:intron 30.0%
!! ATTTGCAAACGTGTTTTTAC+AGG - chr5.2:57731721-57731740 MS.gene61920:intron 30.0%
!! GATTTTCTTGATTTAGGATC+TGG - chr5.2:57730734-57730753 MS.gene61920:intron 30.0%
!!! ACAATCTCTCTTTTTTTGTG+TGG - chr5.2:57731927-57731946 MS.gene61920:intron 30.0%
AAAAAGGAATTACAAGCTGG+GGG + chr5.2:57731235-57731254 None:intergenic 35.0%
AAAAAGGACATGAAGAGCAA+CGG + chr5.2:57730564-57730583 None:intergenic 35.0%
AAACCTGACATGTAAAGGTA+TGG + chr5.2:57732378-57732397 None:intergenic 35.0%
AAAGGAAAGTTTGGATTCGA+CGG + chr5.2:57730329-57730348 None:intergenic 35.0%
AACACTGCTGAGATTCAAAT+TGG - chr5.2:57728451-57728470 MS.gene61920:CDS 35.0%
AACTCTAAACAACACCACTA+CGG + chr5.2:57731118-57731137 None:intergenic 35.0%
AATGTCGAACAAGTAACAGT+TGG - chr5.2:57728478-57728497 MS.gene61920:intron 35.0%
ACAACAAATCAGCATAAGAG+TGG + chr5.2:57731151-57731170 None:intergenic 35.0%
ACAACAACTAATGAGTGTCT+TGG - chr5.2:57728875-57728894 MS.gene61920:intron 35.0%
AGATTATGATGCTCAAAACG+AGG - chr5.2:57731841-57731860 MS.gene61920:intron 35.0%
AGCTGAGTATTATCGTTGTT+TGG - chr5.2:57729857-57729876 MS.gene61920:intron 35.0%
ATATCATCTGAGGGTAACTT+AGG - chr5.2:57732274-57732293 MS.gene61920:intron 35.0%
CATAATCTGATTGCTGAAGA+TGG + chr5.2:57731829-57731848 None:intergenic 35.0%
CATCTTCCACAACCAATTAA+GGG - chr5.2:57731782-57731801 MS.gene61920:intron 35.0%
CTACTAACAACAGTCAATGA+AGG + chr5.2:57729086-57729105 None:intergenic 35.0%
GAAAGGAGAAGAGATGAAAA+AGG + chr5.2:57731963-57731982 None:intergenic 35.0%
GCTTAAAGAAGTTTGGATGT+TGG + chr5.2:57730244-57730263 None:intergenic 35.0%
GTTTCTCTAATTAGTGTCTC+AGG - chr5.2:57729355-57729374 MS.gene61920:intron 35.0%
TAAAGAAGTTTGGATGTTGG+AGG + chr5.2:57730241-57730260 None:intergenic 35.0%
TAATCTGATTGCTGAAGATG+GGG + chr5.2:57731827-57731846 None:intergenic 35.0%
TATCATCTGAGGGTAACTTA+GGG - chr5.2:57732275-57732294 MS.gene61920:intron 35.0%
TGACAGATATAGCAGTTGAA+AGG - chr5.2:57732175-57732194 MS.gene61920:intron 35.0%
! AACCTGACATGTAAAGGTAT+GGG + chr5.2:57732377-57732396 None:intergenic 35.0%
! ACATCTGGCTTAAAGAAGTT+TGG + chr5.2:57730251-57730270 None:intergenic 35.0%
! ATAATAGGTCCAACTTGCTT+GGG - chr5.2:57729477-57729496 MS.gene61920:intron 35.0%
! CTCTTGAGTTGTTGTTAGTT+GGG - chr5.2:57730469-57730488 MS.gene61920:intron 35.0%
! TATAATAGGTCCAACTTGCT+TGG - chr5.2:57729476-57729495 MS.gene61920:intron 35.0%
! TCAAAACGAGGTATTCAACA+TGG - chr5.2:57731853-57731872 MS.gene61920:intron 35.0%
! TCGGGATTTGGAACATAAAT+TGG + chr5.2:57728523-57728542 None:intergenic 35.0%
! TCTGTCGTTTGTTCTTTGAT+CGG - chr5.2:57730901-57730920 MS.gene61920:intron 35.0%
! TCTTGAGTTGTTGTTAGTTG+GGG - chr5.2:57730470-57730489 MS.gene61920:intron 35.0%
! TTGCTCTTCATGTCCTTTTT+CGG - chr5.2:57730564-57730583 MS.gene61920:intron 35.0%
!! ATTTGTTGTATCACCCGTTA+GGG - chr5.2:57731162-57731181 MS.gene61920:intron 35.0%
!! TTAATTTTAGTGTCTCAGGG+AGG + chr5.2:57729280-57729299 None:intergenic 35.0%
!!! CTGCGTTTTTTTTTCCAGTT+TGG - chr5.2:57731058-57731077 MS.gene61920:intron 35.0%
!!! TGGCTTGCTGAAGTTTTTTA+GGG - chr5.2:57730961-57730980 MS.gene61920:intron 35.0%
!!! TTGGCTTGCTGAAGTTTTTT+AGG - chr5.2:57730960-57730979 MS.gene61920:intron 35.0%
AAAAGGAATTACAAGCTGGG+GGG + chr5.2:57731234-57731253 None:intergenic 40.0%
AACAAAAAGCCACCCGAAAA+AGG + chr5.2:57730580-57730599 None:intergenic 40.0%
AACCCATACCTTTACATGTC+AGG - chr5.2:57732372-57732391 MS.gene61920:CDS 40.0%
AACTACGTACAACCCAAATG+TGG + chr5.2:57729003-57729022 None:intergenic 40.0%
AAGGAAAGTTTGGATTCGAC+GGG + chr5.2:57730328-57730347 None:intergenic 40.0%
AGAATCTTGTCGCATTTGTC+TGG - chr5.2:57732230-57732249 MS.gene61920:intron 40.0%
AGATGTTGCTCGAAAGAGTT+TGG - chr5.2:57730798-57730817 MS.gene61920:intron 40.0%
AGTTGAAAGGTTTGAGCTTG+TGG - chr5.2:57732188-57732207 MS.gene61920:intron 40.0%
ATTTCTCTCACTTCGGGATT+TGG + chr5.2:57728535-57728554 None:intergenic 40.0%
CCATCTTCCACAACCAATTA+AGG - chr5.2:57731781-57731800 MS.gene61920:intron 40.0%
CCTTAATTGGTTGTGGAAGA+TGG + chr5.2:57731784-57731803 None:intergenic 40.0%
CTCCATCATTTCTCTCACTT+CGG + chr5.2:57728542-57728561 None:intergenic 40.0%
CTTTAAGCCAGATGTAGATC+CGG - chr5.2:57730256-57730275 MS.gene61920:intron 40.0%
GATTAACATCAACCAAGGTC+CGG + chr5.2:57730705-57730724 None:intergenic 40.0%
GGACTTCGATTGGATTGATT+TGG - chr5.2:57730923-57730942 MS.gene61920:intron 40.0%
GTATAGACCACCTTCATAAG+TGG + chr5.2:57729920-57729939 None:intergenic 40.0%
TAGTTGGGGTTTCGAAACAA+AGG - chr5.2:57730484-57730503 MS.gene61920:intron 40.0%
TCACGATAGACCACTTATGA+AGG - chr5.2:57729907-57729926 MS.gene61920:intron 40.0%
TCACGGTAGACCACTTATAA+AGG + chr5.2:57729690-57729709 None:intergenic 40.0%
TCCATCATTTCTCTCACTTC+GGG + chr5.2:57728541-57728560 None:intergenic 40.0%
TGTTTCCAGGAATAAGAAGC+TGG - chr5.2:57730667-57730686 MS.gene61920:intron 40.0%
TTAGGACCCTTAATTGGTTG+TGG + chr5.2:57731791-57731810 None:intergenic 40.0%
TTCTTATATCGAAGGTCGGA+GGG + chr5.2:57728647-57728666 None:intergenic 40.0%
TTTCTTATATCGAAGGTCGG+AGG + chr5.2:57728648-57728667 None:intergenic 40.0%
! CTGTCGTTTGTTCTTTGATC+GGG - chr5.2:57730902-57730921 MS.gene61920:intron 40.0%
! GAGAGAAATGATGGAGCATA+AGG - chr5.2:57728546-57728565 MS.gene61920:intron 40.0%
! GATCTAGAGCAAGAGATAAC+CGG + chr5.2:57730399-57730418 None:intergenic 40.0%
! GCTCTTGAGTTGTTGTTAGT+TGG - chr5.2:57730468-57730487 MS.gene61920:intron 40.0%
! TCTTGCTCTAGATCTACTCT+TGG - chr5.2:57730405-57730424 MS.gene61920:intron 40.0%
! TGCTCTTCATGTCCTTTTTC+GGG - chr5.2:57730565-57730584 MS.gene61920:intron 40.0%
! TTTGTTGTGAGTTTCGTCTC+CGG - chr5.2:57730377-57730396 MS.gene61920:intron 40.0%
!! CGTGTTTTTACAGGATAAGG+CGG - chr5.2:57731730-57731749 MS.gene61920:intron 40.0%
!! GATTTGTTGTATCACCCGTT+AGG - chr5.2:57731161-57731180 MS.gene61920:intron 40.0%
!! GGTTTGTTAGGACCCTTAAT+TGG + chr5.2:57731797-57731816 None:intergenic 40.0%
!! GTTGTTTTCGTTCTGTTTCC+AGG - chr5.2:57730654-57730673 MS.gene61920:intron 40.0%
!!! GAAGTTTTTTAGGGACCTGT+TGG - chr5.2:57730970-57730989 MS.gene61920:intron 40.0%
AAAGGAATTACAAGCTGGGG+GGG + chr5.2:57731233-57731252 None:intergenic 45.0%
AAAGTTTGGATTCGACGGGA+AGG + chr5.2:57730324-57730343 None:intergenic 45.0%
AACGCTCAGGTCAACCTTTA+AGG - chr5.2:57729506-57729525 MS.gene61920:intron 45.0%
AATGACTACTACCGCCAACA+CGG + chr5.2:57730836-57730855 None:intergenic 45.0%
ACCTTGGATCGTTTCTGTGT+TGG - chr5.2:57730993-57731012 MS.gene61920:intron 45.0%
AGTCTGTGGTTATCTAGGTC+AGG - chr5.2:57731189-57731208 MS.gene61920:intron 45.0%
CGATAGACCACTTATGAAGG+TGG - chr5.2:57729910-57729929 MS.gene61920:intron 45.0%
CGGTAGACCACTTATAAAGG+TGG + chr5.2:57729687-57729706 None:intergenic 45.0%
CTTGTCGCATTTGTCTGGTA+AGG - chr5.2:57732235-57732254 MS.gene61920:intron 45.0%
GCATGGATACGAAACCAAAC+TGG + chr5.2:57731075-57731094 None:intergenic 45.0%
GGTGAGTCCACCTTTATAAG+TGG - chr5.2:57729677-57729696 MS.gene61920:intron 45.0%
GTACAGCGACGAACGAAAAA+CGG + chr5.2:57730438-57730457 None:intergenic 45.0%
GTCGAACAAGTAACAGTTGG+TGG - chr5.2:57728481-57728500 MS.gene61920:intron 45.0%
GTGTCTCAGGGATGATTGTT+AGG - chr5.2:57729368-57729387 MS.gene61920:intron 45.0%
GTTCGAGTCTGTGGTTATCT+AGG - chr5.2:57731184-57731203 MS.gene61920:intron 45.0%
TCCCGAAGTGAGAGAAATGA+TGG - chr5.2:57728537-57728556 MS.gene61920:intron 45.0%
TCTTTGATCGGGACTTCGAT+TGG - chr5.2:57730913-57730932 MS.gene61920:intron 45.0%
TTCCAGGAATAAGAAGCTGG+TGG - chr5.2:57730670-57730689 MS.gene61920:intron 45.0%
! AGGTCAACCTTTAAGGATGC+AGG - chr5.2:57729513-57729532 MS.gene61920:intron 45.0%
! ATAATAATTTGCAGCCGGCG+TGG - chr5.2:57732106-57732125 MS.gene61920:intron 45.0%
! TGGGGTAACACAGGTTTGTT+AGG + chr5.2:57731809-57731828 None:intergenic 45.0%
!! TGGATTGATTTGGCTCGAGA+GGG - chr5.2:57730933-57730952 MS.gene61920:intron 45.0%
!! TTGGATTGATTTGGCTCGAG+AGG - chr5.2:57730932-57730951 MS.gene61920:intron 45.0%
!!! TCTTCATGTCCTTTTTCGGG+TGG - chr5.2:57730568-57730587 MS.gene61920:intron 45.0%
!! AAAAAAAAGAATTTATAATT+AGG - chr5.2:57731283-57731302 MS.gene61920:intron 5.0%
!! AAATTGAATTAAAATAAAAA+AGG + chr5.2:57731251-57731270 None:intergenic 5.0%
!! ATAATAATAATAATAGTAAA+TGG - chr5.2:57729155-57729174 MS.gene61920:intron 5.0%
AAGGAATTACAAGCTGGGGG+GGG + chr5.2:57731232-57731251 None:intergenic 50.0%
AAGGACATGAAGAGCAACGG+TGG + chr5.2:57730561-57730580 None:intergenic 50.0%
ACCACAGACTCGAACCCTAA+CGG + chr5.2:57731179-57731198 None:intergenic 50.0%
AGAGAAGAGAGCGAGAGAAG+CGG + chr5.2:57730175-57730194 None:intergenic 50.0%
CACAACAAACCACCCATTGC+CGG + chr5.2:57730366-57730385 None:intergenic 50.0%
CACCACCAGCTTCTTATTCC+TGG + chr5.2:57730675-57730694 None:intergenic 50.0%
CAGGAATAAGAAGCTGGTGG+TGG - chr5.2:57730673-57730692 MS.gene61920:intron 50.0%
GAAACGATCCAAGGTCCAAC+AGG + chr5.2:57730988-57731007 None:intergenic 50.0%
GATAGACTGCTCTTCCTCGT+CGG - chr5.2:57730506-57730525 MS.gene61920:intron 50.0%
GCCAACACAGAAACGATCCA+AGG + chr5.2:57730997-57731016 None:intergenic 50.0%
GGTAAGACGGTGGTTGGATA+GGG + chr5.2:57730203-57730222 None:intergenic 50.0%
GGTTTCGTATCCATGCGTGT+GGG - chr5.2:57731079-57731098 MS.gene61920:intron 50.0%
GTGTCTCAGGGAGGATTGTT+AGG + chr5.2:57729271-57729290 None:intergenic 50.0%
GTTTGGATGTTGGAGGAGCT+AGG + chr5.2:57730234-57730253 None:intergenic 50.0%
TCTGTCATCAGTTGTGCCGT+TGG - chr5.2:57730869-57730888 MS.gene61920:intron 50.0%
TGCTGAAGATGGGGTAACAC+AGG + chr5.2:57731818-57731837 None:intergenic 50.0%
TGGTTTCGTATCCATGCGTG+TGG - chr5.2:57731078-57731097 MS.gene61920:intron 50.0%
TTTAGGGACCTGTTGGACCT+TGG - chr5.2:57730977-57730996 MS.gene61920:intron 50.0%
! CTAGGAAGGTGACGGTAAGA+CGG + chr5.2:57730216-57730235 None:intergenic 50.0%
!! AAGCTGGTGGTGGTTTGATC+CGG - chr5.2:57730683-57730702 MS.gene61920:intron 50.0%
!!! CCTTTTTCAACCTTTGCCGC+CGG - chr5.2:57730344-57730363 MS.gene61920:intron 50.0%
AACAAACCACCCATTGCCGG+CGG + chr5.2:57730363-57730382 None:intergenic 55.0%
AGAAGAGAGCGAGAGAAGCG+GGG + chr5.2:57730173-57730192 None:intergenic 55.0%
AGGAATTACAAGCTGGGGGG+GGG + chr5.2:57731231-57731250 None:intergenic 55.0%
CCACAGACTCGAACCCTAAC+GGG + chr5.2:57731178-57731197 None:intergenic 55.0%
CCGGCGGCAAAGGTTGAAAA+AGG + chr5.2:57730347-57730366 None:intergenic 55.0%
CGACAGACACAGTCCACCAA+CGG + chr5.2:57730888-57730907 None:intergenic 55.0%
CGGTAAGACGGTGGTTGGAT+AGG + chr5.2:57730204-57730223 None:intergenic 55.0%
CTTCCACTCTGCGTATCCAC+CGG + chr5.2:57732149-57732168 None:intergenic 55.0%
GAGAAGAGAGCGAGAGAAGC+GGG + chr5.2:57730174-57730193 None:intergenic 55.0%
GGATGTTGGAGGAGCTAGGA+AGG + chr5.2:57730230-57730249 None:intergenic 55.0%
GTTCGCGTTCCCAAGCAAGT+TGG + chr5.2:57729489-57729508 None:intergenic 55.0%
GTTCGTCGCTGTACGCAAGT+TGG - chr5.2:57730444-57730463 MS.gene61920:intron 55.0%
TGGAGGAGCTAGGAAGGTGA+CGG + chr5.2:57730224-57730243 None:intergenic 55.0%
TGGTGGTTTGATCCGGACCT+TGG - chr5.2:57730690-57730709 MS.gene61920:intron 55.0%
TTGGATTCGACGGGAAGGAG+AGG + chr5.2:57730319-57730338 None:intergenic 55.0%
! AAGGAGAGGTGGTGACGTCT+TGG + chr5.2:57730305-57730324 None:intergenic 55.0%
! CTCTTCCTCGTCGGTTGCTT+GGG - chr5.2:57730515-57730534 MS.gene61920:intron 55.0%
! GTCATCAGTTGTGCCGTTGG+TGG - chr5.2:57730872-57730891 MS.gene61920:intron 55.0%
! TCTTCCTCGTCGGTTGCTTG+GGG - chr5.2:57730516-57730535 MS.gene61920:intron 55.0%
! TGCGTATCCACCGGTTGGTT+AGG + chr5.2:57732140-57732159 None:intergenic 55.0%
AATTACAAGCTGGGGGGGGG+GGG + chr5.2:57731228-57731247 None:intergenic 60.0%
ATCCATGCGTGTGGGTTCGC+TGG - chr5.2:57731087-57731106 MS.gene61920:intron 60.0%
CAACCGGTGGATACGCAGAG+TGG - chr5.2:57732143-57732162 MS.gene61920:intron 60.0%
CAACCTTTGCCGCCGGCAAT+GGG - chr5.2:57730351-57730370 MS.gene61920:intron 60.0%
CACTCTGCGTATCCACCGGT+TGG + chr5.2:57732145-57732164 None:intergenic 60.0%
CCAGATGTAGATCCGGCCTC+AGG - chr5.2:57730263-57730282 MS.gene61920:intron 60.0%
CCCGTTAGGGTTCGAGTCTG+TGG - chr5.2:57731175-57731194 MS.gene61920:intron 60.0%
CCTGAGGCCGGATCTACATC+TGG + chr5.2:57730266-57730285 None:intergenic 60.0%
GAATTACAAGCTGGGGGGGG+GGG + chr5.2:57731229-57731248 None:intergenic 60.0%
GATGTAGATCCGGCCTCAGG+TGG - chr5.2:57730266-57730285 MS.gene61920:intron 60.0%
GATTCGACGGGAAGGAGAGG+TGG + chr5.2:57730316-57730335 None:intergenic 60.0%
GGAATTACAAGCTGGGGGGG+GGG + chr5.2:57731230-57731249 None:intergenic 60.0%
GGCTGCAACGCCTAACCAAC+CGG - chr5.2:57732127-57732146 MS.gene61920:intron 60.0%
GGTGTGTTGTCGCTCCGTGT+TGG - chr5.2:57730819-57730838 MS.gene61920:intron 60.0%
GTGTTGTCGCTCCGTGTTGG+CGG - chr5.2:57730822-57730841 MS.gene61920:intron 60.0%
TCAACCTTTGCCGCCGGCAA+TGG - chr5.2:57730350-57730369 MS.gene61920:intron 60.0%
TCCATGCGTGTGGGTTCGCT+GGG - chr5.2:57731088-57731107 MS.gene61920:intron 60.0%
TGCAACGCCTAACCAACCGG+TGG - chr5.2:57732130-57732149 MS.gene61920:intron 60.0%
! CTTCCTCGTCGGTTGCTTGG+GGG - chr5.2:57730517-57730536 MS.gene61920:intron 60.0%
! GCTCTTCCTCGTCGGTTGCT+TGG - chr5.2:57730514-57730533 MS.gene61920:intron 60.0%
! GGAAGGTGACGGTAAGACGG+TGG + chr5.2:57730213-57730232 None:intergenic 60.0%
! GGTGACGGTAAGACGGTGGT+TGG + chr5.2:57730209-57730228 None:intergenic 60.0%
!! TTTGGCTCGAGAGGGCGCTT+TGG - chr5.2:57730941-57730960 MS.gene61920:intron 60.0%
ACGCGATCACCACCTGAGGC+CGG + chr5.2:57730278-57730297 None:intergenic 65.0%
AGAGAGCGAGAGAAGCGGGG+TGG + chr5.2:57730170-57730189 None:intergenic 65.0%
AGCGACGCGATCACCACCTG+AGG + chr5.2:57730282-57730301 None:intergenic 65.0%
ATTACAAGCTGGGGGGGGGG+GGG + chr5.2:57731227-57731246 None:intergenic 65.0%
CAACCCCCAAGCAACCGACG+AGG + chr5.2:57730523-57730542 None:intergenic 65.0%
GCTTGGGAACGCGAACGCTC+AGG - chr5.2:57729493-57729512 MS.gene61920:intron 65.0%
! CGTCGGTTGCTTGGGGGTTG+AGG - chr5.2:57730523-57730542 MS.gene61920:intron 65.0%
! GTTAGGCGTTGCAGCCACGC+CGG + chr5.2:57732123-57732142 None:intergenic 65.0%
CCACCCATTGCCGGCGGCAA+AGG + chr5.2:57730357-57730376 None:intergenic 70.0%
CCTTTGCCGCCGGCAATGGG+TGG - chr5.2:57730354-57730373 MS.gene61920:intron 70.0%
GCCCAGCGAACCCACACGCA+TGG + chr5.2:57731092-57731111 None:intergenic 70.0%
TGCGTGTGGGTTCGCTGGGC+TGG - chr5.2:57731092-57731111 MS.gene61920:intron 70.0%
TTACAAGCTGGGGGGGGGGG+GGG + chr5.2:57731226-57731245 None:intergenic 70.0%
!! GTTCGCTGGGCTGGCCGTAG+TGG - chr5.2:57731101-57731120 MS.gene61920:intron 70.0%
TACAAGCTGGGGGGGGGGGG+GGG + chr5.2:57731225-57731244 None:intergenic 75.0%
! ACAAGCTGGGGGGGGGGGGG+GGG + chr5.2:57731224-57731243 None:intergenic 80.0%
! AAGCTGGGGGGGGGGGGGGG+GGG + chr5.2:57731222-57731241 None:intergenic 85.0%
! CAAGCTGGGGGGGGGGGGGG+GGG + chr5.2:57731223-57731242 None:intergenic 85.0%
! AGCTGGGGGGGGGGGGGGGG+GGG + chr5.2:57731221-57731240 None:intergenic 90.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 57728448 57732417 57728448 ID=MS.gene61920
chr5.2 mRNA 57728448 57732417 57728448 ID=MS.gene61920.t1;Parent=MS.gene61920
chr5.2 exon 57732298 57732417 57732298 ID=MS.gene61920.t1.exon1;Parent=MS.gene61920.t1
chr5.2 CDS 57732298 57732417 57732298 ID=cds.MS.gene61920.t1;Parent=MS.gene61920.t1
chr5.2 exon 57731331 57731381 57731331 ID=MS.gene61920.t1.exon2;Parent=MS.gene61920.t1
chr5.2 CDS 57731331 57731381 57731331 ID=cds.MS.gene61920.t1;Parent=MS.gene61920.t1
chr5.2 exon 57729048 57729122 57729048 ID=MS.gene61920.t1.exon3;Parent=MS.gene61920.t1
chr5.2 CDS 57729048 57729122 57729048 ID=cds.MS.gene61920.t1;Parent=MS.gene61920.t1
chr5.2 exon 57728614 57728745 57728614 ID=MS.gene61920.t1.exon4;Parent=MS.gene61920.t1
chr5.2 CDS 57728614 57728745 57728614 ID=cds.MS.gene61920.t1;Parent=MS.gene61920.t1
chr5.2 exon 57728448 57728471 57728448 ID=MS.gene61920.t1.exon5;Parent=MS.gene61920.t1
chr5.2 CDS 57728448 57728471 57728448 ID=cds.MS.gene61920.t1;Parent=MS.gene61920.t1
Gene Sequence

>MS.gene61920

ATGAACACTGCTGAGATTCAAATTGGTCAGAATGTCGAACAAGTAACAGTTGGTGGAAATGTTGAGCAACAACCAATTTATGTTCCAAATCCCGAAGTGAGAGAAATGATGGAGCATAAGGTATGTTTGTAATGTTGTTTATTTATTTAGAATCCAATAAGGTAAAATGCATGTGAATTTATTTTTAACATGTACTCCCTCCGACCTTCGATATAAGAAAGTTTTCTATTTTTTTCGTGCTATTTATAACAAAAAAATATAACTTTTAAAGTATATTTATTGATTTAAAGATTTTTCTAGCCTTCCTTTAATATTTATATATTTTTAATATTAGTGTGTATGTTTAATATTCACAAATTTTTATAAGAGTATATTTGTCAATTTTTTTCTTTTATGAGGGTATATGTGTGAAAACATTCACAAAGTTACAACAACTAATGAGTGTCTTGGTTTGATGTTTTTTTTTTTTCTTCTTATAGTAAGGACTAAAGAGAGTATTTCTTGAAATTCAGTCTCTAATTATATTTTATTCGATACGTTTCATTTTTATTACCACATTTGGGTTGTACGTAGTTCTTAAGAAAGTGATCTATTGAGATGATTTTAATGATAAAATTAATATTGTTTATAAAACACCTTCATTGACTGTTGTTAGTAGACTAGTTAAGTGAAATTCTATATTTAAGGTTATATCAGTGAAAATAATAATAATAATAATAATAGTAAATGGTATATTGATATTCTAAAATGACAATTATTTTGATAAAGAAAAATAACGCAAAAGTGACTAAGAGTTTGACTATAAATGTTTAAAAATATTCCTAACAATCCTCCCTGAGACACTAAAATTAATATTATACTTTTAAGAGTTTGACTATAAATGTTTAAAAATATTTTTATTATATTTGTTTCTCTAATTAGTGTCTCAGGGATGATTGTTAGGAATTTCCTATATTAATTTGCAGTTGAGACTAAGAGTTTGACTATGATTTAGTGGGATCATCGTTTCATCCTCATTAGTTTTCATGTATAATAGGTCCAACTTGCTTGGGAACGCGAACGCTCAGGTCAACCTTTAAGGATGCAGGTATATTTATGCATTTTTAATTTCAAATTTATATCTATGATTGATTTGATGATTACTAGATCCATCCTTACAAATCTATAAGATCATTAGTATTTTCTTGTTGTTTTCTTAGCTATCTTGATATTTAGGATGTCTGTTTGTAGGTGAGTCCACCTTTATAAGTGGTCTACCGTGAACTAATATTTAAAATGATAAAATTTCTCATATTTACGATTAAGACGATAACTTGACTGATGTCTATTCAACACAAAATACATATTTATTTTTTGTTGTTTTAATCAAATAATATCACTTCAATCATTACGATCAATAGTAAACATGAAGCTGAGTATTATCGTTGTTTGGAGTTTCAAATATTTTGGACGTACAAGTTCACGATAGACCACTTATGAAGGTGGTCTATACTAGATCAATTTCTCTATATGTATACATTGACTTTTGAGTTTATTTTTGGACATAAGAGACTTTATAAGATTCCTTGAGATGGTTTATAGAATCAATTTCTAACATGTTCATACATTGTTTCATATTACTTGTAAGCTTTTAGGATAGCTTATGAAAAACTCAAAATTTATATAACACTCGTCTGATAGAAATATCTTATATTATACAAGAATTTATAATTAGGCAGACCACCCCGCTTCTCTCGCTCTCTTCTCTCTCTACCCTATCCAACCACCGTCTTACCGTCACCTTCCTAGCTCCTCCAACATCCAAACTTCTTTAAGCCAGATGTAGATCCGGCCTCAGGTGGTGATCGCGTCGCTCCAAGACGTCACCACCTCTCCTTCCCGTCGAATCCAAACTTTCCTTTTTCAACCTTTGCCGCCGGCAATGGGTGGTTTGTTGTGAGTTTCGTCTCCGGTTATCTCTTGCTCTAGATCTACTCTTGGTTTGTCGCCGTTTTTCGTTCGTCGCTGTACGCAAGTTGGTGCTCTTGAGTTGTTGTTAGTTGGGGTTTCGAAACAAAGGATAGACTGCTCTTCCTCGTCGGTTGCTTGGGGGTTGAGGTCGTAGCTAGCGCCACCGTTGCTCTTCATGTCCTTTTTCGGGTGGCTTTTTGTTTGCGACGACTCTTTTTGTGCTTTCTGTTTTGTTTGTGTGATGTACTGTATGTTTGTTGTTTTCGTTCTGTTTCCAGGAATAAGAAGCTGGTGGTGGTTTGATCCGGACCTTGGTTGATGTTAATCTTAAATCTAGATTTTCTTGATTTAGGATCTGGTTACAGAAAATTATGTTTCATCTGTGTGGATTTGATTTGACAGATGTTGCTCGAAAGAGTTTGGTGTGTTGTCGCTCCGTGTTGGCGGTAGTAGTCATTACTAACTGTTTAATCTGTCATCAGTTGTGCCGTTGGTGGACTGTGTCTGTCGTTTGTTCTTTGATCGGGACTTCGATTGGATTGATTTGGCTCGAGAGGGCGCTTTGGCTTGCTGAAGTTTTTTAGGGACCTGTTGGACCTTGGATCGTTTCTGTGTTGGCAGACGATTCATACGCCTACCTTGATACTCTAAACATTCATGCTGCGTTTTTTTTTCCAGTTTGGTTTCGTATCCATGCGTGTGGGTTCGCTGGGCTGGCCGTAGTGGTGTTGTTTAGAGTTGTTTTATTTACCACTCTTATGCTGATTTGTTGTATCACCCGTTAGGGTTCGAGTCTGTGGTTATCTAGGTCAGGTTCATGCCCCCCCCCCCCCCCCCCCAGCTTGTAATTCCTTTTTTATTTTAATTCAATTTCTATTTTGCTTAAAAAAAAAGAATTTATAATTAGGCATATATGATCAGTGATGAATTAATTTAGGCAAAATTACATTTTTAGTCCCTTAACTTAATTTCAGGTAATAGTTTGATCCTTTATCTTTTTTTCATTTTAATTTGGTCCTTTTTGTCCATTTTCATATGAATTTTTAAGCTTAAAATTCATGATTTTATTTTCTTATGGTCTTTCAGTCTTCAAATCTATGATCCTCGTCTATGTTTACTAAGAATATTAGATTTGAAGCTTGAAAATGGAGAAAAAGGACCAAATTAAAATGAAAAAAAGATAAAGGACCAAACTATTACCTGAAATTAAGTTAAGGGACTAAAAATGTAATTTTGCCTAAATTAATTCATCACTGATCATATATGCCTGAAATTAATAGTAGTAGTTTTCTCTAGGCTTTAACTTCTAATCAAACGTTTGATATTTGCAAACGTGTTTTTACAGGATAAGGCGGCAATAGACTACTATAATGTTGTGTTACGCCATCTTCCACAACCAATTAAGGGTCCTAACAAACCTGTGTTACCCCATCTTCAGCAATCAGATTATGATGCTCAAAACGAGGTATTCAACATGGTGTATATTTTAAGTGATTAACAAAGCAAATAAATATTTGATATCTTCATCCACAATCTCTCTTTTTTTGTGTGGAATCTTTTATCCTTTTTCATCTCTTCTCCTTTCTCATAGTATTACATTCTCTTTCTTTAAACTTTTTCTATCTTTTTCATACACATTCTGCATAAATACAAGAATATATTATTGTTAGAGTCAATTCCTTACTTTTCATTGAATGCTTATGTACGAATAATAATTTGCAGCCGGCGTGGCTGCAACGCCTAACCAACCGGTGGATACGCAGAGTGGAAGCAATGATGACAGATATAGCAGTTGAAAGGTTTGAGCTTGTGGATGACAGTCTTGAAAATGAAGAATCTTGTCGCATTTGTCTGGTAAGGTTTTTTTACGTTTTCAATATCATCTGAGGGTAACTTAGGGCAAGCATGTTACTTTAAATCAATTTCAATTTCAATTTCGTTCTATATACTCTCTCTAATGAAAATAAAAAAAAAAACCCATACCTTTACATGTCAGGTTTATATGTTGGTTTTTTTCTAA
Protein sequence

>MS.gene61920.t1

MNTAEIQIGQNVEQVTVGGNVEQQPIYVPNPEVREMMEHKVQLAWERERSGQPLRMQDKAAIDYYNVVLRHLPQPIKGPNKPPAWLQRLTNRWIRRVEAMMTDIAVERFELVDDSLENEESCRICLVYMLVFF