AlfalfaGEDB | MS.gene61921

M. sativa cultivar XinJiangDaYe / MS.gene61921

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61921.t1	RHN56340.1	88	158	19	0	1	158	1	158	4.80E-71	277.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61921.t1	A0A396HSM2	88.0	158	19	0	1	158	1	158	3.4e-71	277.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene61921	MS.gene015160	PPI
MS.gene61921	MS.gene94533	PPI
MS.gene61921	MS.gene52667	PPI
MS.gene61921	MS.gene010343	PPI
MS.gene61921	MS.gene93489	PPI
MS.gene61921	MS.gene28214	PPI
MS.gene61921	MS.gene79416	PPI
MS.gene61921	MS.gene94532	PPI
MS.gene61921	MS.gene017970	PPI
MS.gene61921	MS.gene97792	PPI
MS.gene61921	MS.gene047623	PPI
MS.gene61921	MS.gene055988	PPI
MS.gene61921	MS.gene017995	PPI
MS.gene61921	MS.gene041075	PPI
MS.gene61921	MS.gene93488	PPI
MS.gene61921	MS.gene94534	PPI
MS.gene61921	MS.gene93487	PPI
MS.gene61921	MS.gene94527	PPI
MS.gene61921	MS.gene94530	PPI
MS.gene61921	MS.gene79417	PPI
MS.gene61921	MS.gene28215	PPI
MS.gene61921	MS.gene94537	PPI
MS.gene61921	MS.gene28213	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61921.t1	MTR_5g068000	88.889	153	17	0	2	154	1	153	1.64e-90	264

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 39 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCTCCAATCAACCTTTA+AGG	0.240288	5.2:-57715218	MS.gene61921:CDS
AAATTTACCTGCGACCTTAA+AGG	0.244607	5.2:+57715204	None:intergenic
GAACAACAAGTAACAATTAA+TGG	0.277286	5.2:-57715565	MS.gene61921:CDS
TTTGACGTTCCCAAGCAATT+TGG	0.318565	5.2:+57715238	None:intergenic
TCCATCGTTTCTCTCACTTT+AGG	0.331799	5.2:+57715508	None:intergenic
CTGCGACCTTAAAGGTTGAT+TGG	0.360105	5.2:+57715212	None:intergenic
CATCTTCCACAACCGATTAA+TGG	0.364330	5.2:-57714956	MS.gene61921:CDS
CTTCAAGCAATTTCAAAAGT+AGG	0.364624	5.2:-57715735	MS.gene61921:CDS
GTGACGAAGACGTTCTCTTT+CGG	0.384876	5.2:+57715636	None:intergenic
TGGGGTAACACTGGTTGGTT+TGG	0.391705	5.2:+57714932	None:intergenic
GAGAGAAACGATGGAGTATA+TGG	0.392591	5.2:-57715500	MS.gene61921:intron
GGTTGGTTTGGACCATTAAT+CGG	0.399092	5.2:+57714944	None:intergenic
ATAATTTAATTGTTGAAGAT+GGG	0.408228	5.2:+57714913	None:intergenic
GATAATTTAATTGTTGAAGA+TGG	0.408311	5.2:+57714912	None:intergenic
TATTATAGGTCCAAATTGCT+TGG	0.412627	5.2:-57715248	MS.gene61921:intron
GTTTCTCTCACTTTAGGATC+AGG	0.415420	5.2:+57715514	None:intergenic
TTCAAGCAATTTCAAAAGTA+GGG	0.416269	5.2:-57715734	MS.gene61921:CDS
TGTTCAACAATCTGACCAAC+TGG	0.418248	5.2:+57715583	None:intergenic
AAAAGCTCTGGGTACATAGC+TGG	0.446339	5.2:+57715706	None:intergenic
GGCTGCAGAAACTTAACAAC+CGG	0.456089	5.2:-57714756	MS.gene61921:CDS
CTTCTACTTTGCGTATCCAC+CGG	0.472608	5.2:+57714737	None:intergenic
CATTAATCGGTTGTGGAAGA+TGG	0.480861	5.2:+57714957	None:intergenic
ATTATAGGTCCAAATTGCTT+GGG	0.489909	5.2:-57715247	MS.gene61921:intron
TTTGGACCATTAATCGGTTG+TGG	0.503702	5.2:+57714950	None:intergenic
TCACGTAGATATTGAAAGAC+TGG	0.509822	5.2:+57715673	None:intergenic
CAATCAACCTTTAAGGTCGC+AGG	0.515350	5.2:-57715211	MS.gene61921:intron
AAGAACATTATGATGTCTCT+AGG	0.527328	5.2:-57715778	None:intergenic
GAAATTACTAAGATTCCAGT+TGG	0.554578	5.2:-57715598	MS.gene61921:CDS
TAATTTAATTGTTGAAGATG+GGG	0.559952	5.2:+57714914	None:intergenic
AAATTATCATGCTCAAAATG+AGG	0.579687	5.2:-57714897	MS.gene61921:intron
GAAGATGGGGTAACACTGGT+TGG	0.584025	5.2:+57714927	None:intergenic
ATGTCTCTAGGTCGAACTGA+AGG	0.617564	5.2:-57715766	MS.gene61921:CDS
TGTTGAAGATGGGGTAACAC+TGG	0.621401	5.2:+57714923	None:intergenic
TCCTAAAGTGAGAGAAACGA+TGG	0.622912	5.2:-57715509	MS.gene61921:CDS
GCTCTGGGTACATAGCTGGA+AGG	0.644070	5.2:+57715710	None:intergenic
AATAACAATATATACAGCCA+CGG	0.647803	5.2:-57714780	MS.gene61921:CDS
TGCAGAAACTTAACAACCGG+TGG	0.674360	5.2:-57714753	MS.gene61921:CDS
TTAAGTTTCTGCAGCCACCG+TGG	0.730006	5.2:+57714763	None:intergenic
AACAATATATACAGCCACGG+TGG	0.744149	5.2:-57714777	MS.gene61921:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAATTTAATTGTTGAAGAT+GGG	+	chr5.2:57715599-57715618	None:intergenic	15.0%
!!	ATATGTTACATTTACTAATT+AGG	-	chr5.2:57715171-57715190	MS.gene61921:intron	15.0%
!!	TAATTAATAGTTGCAATATA+AGG	-	chr5.2:57715136-57715155	MS.gene61921:intron	15.0%
!!	TATGTTACATTTACTAATTA+GGG	-	chr5.2:57715172-57715191	MS.gene61921:intron	15.0%
!!	TTATATTGCAACTATTAATT+AGG	+	chr5.2:57715137-57715156	None:intergenic	15.0%
!!	TTATTCTTCAATTAAAATCA+AGG	+	chr5.2:57715714-57715733	None:intergenic	15.0%
!!!	TTGTTGATTTAATATACATA+AGG	-	chr5.2:57715372-57715391	MS.gene61921:intron	15.0%
!!	GATAATTTAATTGTTGAAGA+TGG	+	chr5.2:57715600-57715619	None:intergenic	20.0%
!!	TAATTTAATTGTTGAAGATG+GGG	+	chr5.2:57715598-57715617	None:intergenic	20.0%
!!	TCTAATCAATTATTTGGTAT+TGG	-	chr5.2:57715475-57715494	MS.gene61921:intron	20.0%
!!	TTAACGTCTAATCAATTATT+TGG	-	chr5.2:57715469-57715488	MS.gene61921:intron	20.0%
!!!	AATCTTCTAAAGTAAATTCA+TGG	-	chr5.2:57715056-57715075	MS.gene61921:intron	20.0%
!	AAATTATCATGCTCAAAATG+AGG	-	chr5.2:57715612-57715631	MS.gene61921:CDS	25.0%
!	AATAACAATATATACAGCCA+CGG	-	chr5.2:57715729-57715748	MS.gene61921:CDS	25.0%
!	ATAGTTGCAATATAAGGAAA+AGG	-	chr5.2:57715142-57715161	MS.gene61921:intron	25.0%
!	GAACAACAAGTAACAATTAA+TGG	-	chr5.2:57714944-57714963	MS.gene61921:CDS	25.0%
!	GTGTGTATTTATTTGAATCT+TGG	-	chr5.2:57715091-57715110	MS.gene61921:intron	25.0%
!	TTCAAGCAATTTCAAAAGTA+GGG	-	chr5.2:57714775-57714794	MS.gene61921:CDS	25.0%
!!	AAAGTTTCACTCTTGTTTAA+TGG	-	chr5.2:57715204-57715223	MS.gene61921:intron	25.0%
!!	AAGTTTCACTCTTGTTTAAT+GGG	-	chr5.2:57715205-57715224	MS.gene61921:intron	25.0%
!!!	AAATTCATGGAATTTGTTGT+TGG	-	chr5.2:57715069-57715088	MS.gene61921:intron	25.0%
	ATCTACGTGAGACTAAAAAA+AGG	-	chr5.2:57714849-57714868	MS.gene61921:intron	30.0%
	ATTATAGGTCCAAATTGCTT+GGG	-	chr5.2:57715262-57715281	MS.gene61921:intron	30.0%
	CACTAGTTATCGTGTATTAT+AGG	-	chr5.2:57715247-57715266	MS.gene61921:intron	30.0%
	CTTCAAGCAATTTCAAAAGT+AGG	-	chr5.2:57714774-57714793	MS.gene61921:CDS	30.0%
	GAAATTACTAAGATTCCAGT+TGG	-	chr5.2:57714911-57714930	MS.gene61921:CDS	30.0%
	TATTATAGGTCCAAATTGCT+TGG	-	chr5.2:57715261-57715280	MS.gene61921:intron	30.0%
	TCTACGTGAGACTAAAAAAA+GGG	-	chr5.2:57714850-57714869	MS.gene61921:intron	30.0%
	AAAGCTCCAATCAACCTTTA+AGG	-	chr5.2:57715291-57715310	MS.gene61921:intron	35.0%
	AAATTTACCTGCGACCTTAA+AGG	+	chr5.2:57715308-57715327	None:intergenic	35.0%
	GACTAGAGAAAACTACGATT+TGG	+	chr5.2:57715448-57715467	None:intergenic	35.0%
	GATCATCTTCAAAAAGCTCT+GGG	+	chr5.2:57714817-57714836	None:intergenic	35.0%
	TCACGTAGATATTGAAAGAC+TGG	+	chr5.2:57714839-57714858	None:intergenic	35.0%
	AACAATATATACAGCCACGG+TGG	-	chr5.2:57715732-57715751	MS.gene61921:CDS	40.0%
	CATCTTCCACAACCGATTAA+TGG	-	chr5.2:57715553-57715572	MS.gene61921:CDS	40.0%
	CATTAATCGGTTGTGGAAGA+TGG	+	chr5.2:57715555-57715574	None:intergenic	40.0%
	GAGAGAAACGATGGAGTATA+TGG	-	chr5.2:57715009-57715028	MS.gene61921:intron	40.0%
	GGATCATCTTCAAAAAGCTC+TGG	+	chr5.2:57714818-57714837	None:intergenic	40.0%
	TCCATCGTTTCTCTCACTTT+AGG	+	chr5.2:57715004-57715023	None:intergenic	40.0%
	TCCTAAAGTGAGAGAAACGA+TGG	-	chr5.2:57715000-57715019	MS.gene61921:intron	40.0%
	TCTGATCCTGAAAAACTCGT+TGG	+	chr5.2:57715501-57715520	None:intergenic	40.0%
	TGTTCAACAATCTGACCAAC+TGG	+	chr5.2:57714929-57714948	None:intergenic	40.0%
	TTTGACGTTCCCAAGCAATT+TGG	+	chr5.2:57715274-57715293	None:intergenic	40.0%
	TTTGGACCATTAATCGGTTG+TGG	+	chr5.2:57715562-57715581	None:intergenic	40.0%
!	TATTGGCCAACGAGTTTTTC+AGG	-	chr5.2:57715492-57715511	MS.gene61921:intron	40.0%
!!	GGTTGGTTTGGACCATTAAT+CGG	+	chr5.2:57715568-57715587	None:intergenic	40.0%
!!	GTTTCTCTCACTTTAGGATC+AGG	+	chr5.2:57714998-57715017	None:intergenic	40.0%
!!!	ACGAGTTTTTCAGGATCAGA+CGG	-	chr5.2:57715501-57715520	MS.gene61921:CDS	40.0%
	ATGTCTCTAGGTCGAACTGA+AGG	-	chr5.2:57714743-57714762	MS.gene61921:CDS	45.0%
	GGCTGCAGAAACTTAACAAC+CGG	-	chr5.2:57715753-57715772	MS.gene61921:CDS	45.0%
	GTGACGAAGACGTTCTCTTT+CGG	+	chr5.2:57714876-57714895	None:intergenic	45.0%
	TGCAGAAACTTAACAACCGG+TGG	-	chr5.2:57715756-57715775	MS.gene61921:CDS	45.0%
	TGTTGAAGATGGGGTAACAC+TGG	+	chr5.2:57715589-57715608	None:intergenic	45.0%
!	CTGCGACCTTAAAGGTTGAT+TGG	+	chr5.2:57715300-57715319	None:intergenic	45.0%
!!	AAAAGCTCTGGGTACATAGC+TGG	+	chr5.2:57714806-57714825	None:intergenic	45.0%
!!	CAATCAACCTTTAAGGTCGC+AGG	-	chr5.2:57715298-57715317	MS.gene61921:intron	45.0%
	GAAGATGGGGTAACACTGGT+TGG	+	chr5.2:57715585-57715604	None:intergenic	50.0%
	TTAAGTTTCTGCAGCCACCG+TGG	+	chr5.2:57715749-57715768	None:intergenic	50.0%
!	TGGGGTAACACTGGTTGGTT+TGG	+	chr5.2:57715580-57715599	None:intergenic	50.0%
!	GCTCTGGGTACATAGCTGGA+AGG	+	chr5.2:57714802-57714821	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	57714740	57715791	57714740	ID=MS.gene61921
chr5.2	mRNA	57714740	57715791	57714740	ID=MS.gene61921.t1;Parent=MS.gene61921
chr5.2	exon	57715501	57715791	57715501	ID=MS.gene61921.t1.exon1;Parent=MS.gene61921.t1
chr5.2	CDS	57715501	57715791	57715501	ID=cds.MS.gene61921.t1;Parent=MS.gene61921.t1
chr5.2	exon	57715212	57715262	57715212	ID=MS.gene61921.t1.exon2;Parent=MS.gene61921.t1
chr5.2	CDS	57715212	57715262	57715212	ID=cds.MS.gene61921.t1;Parent=MS.gene61921.t1
chr5.2	exon	57714898	57715017	57714898	ID=MS.gene61921.t1.exon3;Parent=MS.gene61921.t1
chr5.2	CDS	57714898	57715017	57714898	ID=cds.MS.gene61921.t1;Parent=MS.gene61921.t1
chr5.2	exon	57714740	57714802	57714740	ID=MS.gene61921.t1.exon4;Parent=MS.gene61921.t1
chr5.2	CDS	57714740	57714802	57714740	ID=cds.MS.gene61921.t1;Parent=MS.gene61921.t1

Gene Sequence

>MS.gene61921

ATGATGTCTCTAGGTCGAACTGAAGGTGATAGTTCTTCAAGCAATTTCAAAAGTAGGGACCTTCCAGCTATGTACCCAGAGCTTTTTGAAGATGATCCAGTCTTTCAATATCTACGTGAGACTAAAAAAAGGGCCGAAAGAGAACGTCTTCGTCACATGCGTAAGTCAGTTGAAATTACTAAGATTCCAGTTGGTCAGATTGTTGAACAACAAGTAACAATTAATGGAAATGTTGAGCTACGACGAATTTATGTTCCTGATCCTAAAGTGAGAGAAACGATGGAGTATATGGTATGTATGTTTATTATTTATTTATAATCTTCTAAAGTAAATTCATGGAATTTGTTGTTGGTGTGTATTTATTTGAATCTTGGATTATATTTAACAATTACAGCCTAATTAATAGTTGCAATATAAGGAAAAGGAATACGATATGTTACATTTACTAATTAGGGTTGATACTTAAAGTTTCACTCTTGTTTAATGGGATCATCATTTCATCTTCATCACTAGTTATCGTGTATTATAGGTCCAAATTGCTTGGGAACGTCAAAGCTCCAATCAACCTTTAAGGTCGCAGGTAAATTTTCACATAACTATGTATGTATCTTTTTATATTAAATGTATATCTATTGTTGATTTAATATACATAAGGCATATATATGATATGTGATTATGATATAACTGCATAATTAATTTCTTTATCCAAATCGTAGTTTTCTCTAGTCATTAACGTCTAATCAATTATTTGGTATTGGCCAACGAGTTTTTCAGGATCAGACGGCAATAGACTACTATAATGTTGTGTTGTGCCATCTTCCACAACCGATTAATGGTCCAAACCAACCAGTGTTACCCCATCTTCAACAATTAAATTATCATGCTCAAAATGAGGTATTACATTGTCTCTTTCTTTTTTTACTTTTTATTATTCTTTGTGCTTAAATTAAAGAGTTATTGTTAGATAAATTCCTTGATTTTAATTGAAGAATAACAATATATACAGCCACGGTGGCTGCAGAAACTTAACAACCGGTGGATACGCAAAGTAG

Protein sequence

>MS.gene61921.t1

MMSLGRTEGDSSSSNFKSRDLPAMYPELFEDDPVFQYLRETKKRAERERLRHMRKSVEITKIPVGQIVEQQVTINGNVELRRIYVPDPKVRETMEYMVQIAWERQSSNQPLRSQDQTAIDYYNVVLCHLPQPINGPNQPVLPHLQQLNYHAQNENNNIYSHGGCRNLTTGGYAK