AlfalfaGEDB | MS.gene61922

M. sativa cultivar XinJiangDaYe / MS.gene61922

KEGG: K12233
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61922.t1	RHN56340.1	80.5	113	19	1	1	110	1	113	6.60E-42	179.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61922.t1	A0A396HSM2	80.5	113	19	1	1	110	1	113	4.7e-42	179.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene61922	MS.gene93488	PPI
MS.gene93489	MS.gene61922	PPI
MS.gene61922	MS.gene94532	PPI
MS.gene010343	MS.gene61922	PPI
MS.gene61922	MS.gene017970	PPI
MS.gene61922	MS.gene97792	PPI
MS.gene61922	MS.gene055988	PPI
MS.gene61922	MS.gene017995	PPI
MS.gene61922	MS.gene041075	PPI
MS.gene61922	MS.gene79417	PPI
MS.gene61922	MS.gene94534	PPI
MS.gene61922	MS.gene94527	PPI
MS.gene79416	MS.gene61922	PPI
MS.gene61922	MS.gene94530	PPI
MS.gene61922	MS.gene28215	PPI
MS.gene61922	MS.gene94537	PPI
MS.gene28213	MS.gene61922	PPI
MS.gene61922	MS.gene015160	PPI
MS.gene61922	MS.gene94533	PPI
MS.gene61922	MS.gene52667	PPI
MS.gene047623	MS.gene61922	PPI
MS.gene61922	MS.gene28214	PPI
MS.gene93487	MS.gene61922	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61922.t1	MTR_5g068000	80.357	112	19	1	2	110	1	112	4.95e-54	169

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 27 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCTCCAATCAACCTTTA+AGG	0.240288	5.2:-57708206	MS.gene61922:CDS
AAATATACCTACGACCTTAA+AGG	0.273782	5.2:+57708192	None:intergenic
CTTCAAGCAACTTCCAAATT+AGG	0.286862	5.2:-57708722	MS.gene61922:CDS
TTGAGGTCTCACGTAGATAT+TGG	0.298885	5.2:+57708652	None:intergenic
TAGCTGGTAGGTGCCTAATT+TGG	0.313434	5.2:+57708709	None:intergenic
GTTTCTCTCACTTTAGGATT+AGG	0.313484	5.2:+57708510	None:intergenic
TTTGACGTTCCCAAGCAATT+TGG	0.318565	5.2:+57708226	None:intergenic
TCCATCGTTTCTCTCACTTT+AGG	0.331799	5.2:+57708504	None:intergenic
CTACGACCTTAAAGGTTGAT+TGG	0.389759	5.2:+57708200	None:intergenic
GAGAGAAACGATGGAGTATA+TGG	0.405916	5.2:-57708496	MS.gene61922:intron
TATTATAGGTCCAAATTGCT+TGG	0.412627	5.2:-57708236	MS.gene61922:intron
GGAATCTTAGCAGTTTCAAT+TGG	0.418046	5.2:+57708600	None:intergenic
TGTTCAACAATCTGACCAAC+TGG	0.418248	5.2:+57708579	None:intergenic
GGATCATCTTCAAATAGCTC+TGG	0.423511	5.2:+57708681	None:intergenic
ATTATAGGTCCAAATTGCTT+GGG	0.489909	5.2:-57708235	MS.gene61922:intron
AGGAGGACATTCTCTTCTTG+AGG	0.497966	5.2:+57708635	None:intergenic
AATAGCTCTGGGTACATAGC+TGG	0.501323	5.2:+57708693	None:intergenic
TCACGTAGATATTGGAAGAC+TGG	0.509822	5.2:+57708660	None:intergenic
GAAAACATTATGATGTCTCT+AGG	0.520508	5.2:-57708765	None:intergenic
GATCATCTTCAAATAGCTCT+GGG	0.557026	5.2:+57708682	None:intergenic
GAAACTGCTAAGATTCCAGT+TGG	0.575621	5.2:-57708594	MS.gene61922:CDS
TCCTAAAGTGAGAGAAACGA+TGG	0.602985	5.2:-57708505	MS.gene61922:CDS
CAATCAACCTTTAAGGTCGT+AGG	0.609797	5.2:-57708199	None:intergenic
TCAATTGGCTTACGCATGTG+AGG	0.611366	5.2:+57708615	None:intergenic
ATGTCTCTAGGTCGAACTGA+AGG	0.617564	5.2:-57708753	MS.gene61922:CDS
GCTCTGGGTACATAGCTGGT+AGG	0.632647	5.2:+57708697	None:intergenic
ATTGGCTTACGCATGTGAGG+AGG	0.637092	5.2:+57708618	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAATTTCAATATAAGAAAA+AGG	-	chr5.2:57708595-57708614	MS.gene61922:CDS	10.0%
!!	ACATGTTACATTTACTAATT+AGG	-	chr5.2:57708630-57708649	MS.gene61922:CDS	20.0%
!!	CATGTTACATTTACTAATTA+GGG	-	chr5.2:57708631-57708650	MS.gene61922:CDS	20.0%
!!	GAACAACAAGTAAAAATTAA+CGG	-	chr5.2:57708395-57708414	MS.gene61922:intron	20.0%
!	AATCCTGTAAAGTAAATTCA+TGG	-	chr5.2:57708509-57708528	MS.gene61922:CDS	25.0%
!	ATTCCATGAATTTACTTTAC+AGG	+	chr5.2:57708515-57708534	None:intergenic	25.0%
!!	AAAGTTTGACTCTTGTTTAA+TGG	-	chr5.2:57708663-57708682	MS.gene61922:CDS	25.0%
!!	AAGTTTGACTCTTGTTTAAT+GGG	-	chr5.2:57708664-57708683	MS.gene61922:CDS	25.0%
!!!	AAATTCATGGAATTTGTTGT+TGG	-	chr5.2:57708522-57708541	MS.gene61922:CDS	25.0%
	ATTATAGGTCCAAATTGCTT+GGG	-	chr5.2:57708721-57708740	MS.gene61922:CDS	30.0%
	GCGTGTATTTATTTGAATCT+TGG	-	chr5.2:57708544-57708563	MS.gene61922:CDS	30.0%
	TATTATAGGTCCAAATTGCT+TGG	-	chr5.2:57708720-57708739	MS.gene61922:CDS	30.0%
!!!	CACTAGTTTTCGTGTATTAT+AGG	-	chr5.2:57708706-57708725	MS.gene61922:CDS	30.0%
	AAAGCTCCAATCAACCTTTA+AGG	-	chr5.2:57708750-57708769	MS.gene61922:CDS	35.0%
	CTTCAAGCAACTTCCAAATT+AGG	-	chr5.2:57708234-57708253	MS.gene61922:intron	35.0%
	GATCATCTTCAAATAGCTCT+GGG	+	chr5.2:57708277-57708296	None:intergenic	35.0%
	GGAATCTTAGCAGTTTCAAT+TGG	+	chr5.2:57708359-57708378	None:intergenic	35.0%
!!	GTTTCTCTCACTTTAGGATT+AGG	+	chr5.2:57708449-57708468	None:intergenic	35.0%
	GAAACTGCTAAGATTCCAGT+TGG	-	chr5.2:57708362-57708381	MS.gene61922:intron	40.0%
	GAGAGAAACGATGGAGTATA+TGG	-	chr5.2:57708460-57708479	MS.gene61922:intron	40.0%
	GGATCATCTTCAAATAGCTC+TGG	+	chr5.2:57708278-57708297	None:intergenic	40.0%
	TCACGTAGATATTGGAAGAC+TGG	+	chr5.2:57708299-57708318	None:intergenic	40.0%
	TCCATCGTTTCTCTCACTTT+AGG	+	chr5.2:57708455-57708474	None:intergenic	40.0%
	TCCTAAAGTGAGAGAAACGA+TGG	-	chr5.2:57708451-57708470	MS.gene61922:intron	40.0%
	TGTTCAACAATCTGACCAAC+TGG	+	chr5.2:57708380-57708399	None:intergenic	40.0%
	TTGAGGTCTCACGTAGATAT+TGG	+	chr5.2:57708307-57708326	None:intergenic	40.0%
	TTTGACGTTCCCAAGCAATT+TGG	+	chr5.2:57708733-57708752	None:intergenic	40.0%
!	CTACGACCTTAAAGGTTGAT+TGG	+	chr5.2:57708759-57708778	None:intergenic	40.0%
	AGGAGGACATTCTCTTCTTG+AGG	+	chr5.2:57708324-57708343	None:intergenic	45.0%
	ATGTCTCTAGGTCGAACTGA+AGG	-	chr5.2:57708203-57708222	MS.gene61922:CDS	45.0%
	TAGCTGGTAGGTGCCTAATT+TGG	+	chr5.2:57708250-57708269	None:intergenic	45.0%
!	TCAATTGGCTTACGCATGTG+AGG	+	chr5.2:57708344-57708363	None:intergenic	45.0%
!!	AATAGCTCTGGGTACATAGC+TGG	+	chr5.2:57708266-57708285	None:intergenic	45.0%
!!	TTATATTGAAATTATTAATT+AGG	+	chr5.2:57708590-57708609	None:intergenic	5.0%
!	ATTGGCTTACGCATGTGAGG+AGG	+	chr5.2:57708341-57708360	None:intergenic	50.0%
!	GCTCTGGGTACATAGCTGGT+AGG	+	chr5.2:57708262-57708281	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	57708200	57708778	57708200	ID=MS.gene61922
chr5.2	mRNA	57708200	57708778	57708200	ID=MS.gene61922.t1;Parent=MS.gene61922
chr5.2	exon	57708497	57708778	57708497	ID=MS.gene61922.t1.exon1;Parent=MS.gene61922.t1
chr5.2	CDS	57708497	57708778	57708497	ID=cds.MS.gene61922.t1;Parent=MS.gene61922.t1
chr5.2	exon	57708200	57708250	57708200	ID=MS.gene61922.t1.exon2;Parent=MS.gene61922.t1
chr5.2	CDS	57708200	57708250	57708200	ID=cds.MS.gene61922.t1;Parent=MS.gene61922.t1

Gene Sequence

>MS.gene61922

ATGATGTCTCTAGGTCGAACTGAAGGTGATAATTCTTCAAGCAACTTCCAAATTAGGCACCTACCAGCTATGTACCCAGAGCTATTTGAAGATGATCCAGTCTTCCAATATCTACGTGAGACCTCAAGAAGAGAATGTCCTCCTCACATGCGTAAGCCAATTGAAACTGCTAAGATTCCAGTTGGTCAGATTGTTGAACAACAAGTAAAAATTAACGGAAATGTTGAGCTACAACGAATTTATGTTCCTAATCCTAAAGTGAGAGAAACGATGGAGTATATGGTACGTATATGATTATTATTTATTTATAATCCTGTAAAGTAAATTCATGGAATTTGTTGTTGGCGTGTATTTATTTGAATCTTGGATTATATTTAACAATTACAACCTAATTAATAATTTCAATATAAGAAAAAGGAAGAGAAATACGACATGTTACATTTACTAATTAGGGTTTATACTCAAAGTTTGACTCTTGTTTAATGGGATCATCATTTCATCTTCATCACTAGTTTTCGTGTATTATAGGTCCAAATTGCTTGGGAACGTCAAAGCTCCAATCAACCTTTAAGGTCGTAG

Protein sequence

>MS.gene61922.t1

MMSLGRTEGDNSSSNFQIRHLPAMYPELFEDDPVFQYLRETSRRECPPHMRKPIETAKIPVGQIVEQQVKINGNVELQRIYVPNPKVRETMEYMVQIAWERQSSNQPLRS