Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62076.t1 | AES92608.2 | 88.5 | 157 | 18 | 0 | 1 | 157 | 87 | 243 | 4.90E-69 | 270.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62076.t1 | Q6PL11 | 34.4 | 160 | 80 | 2 | 48 | 186 | 8 | 163 | 2.4e-11 | 70.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62076.t1 | G7JI21 | 88.5 | 157 | 18 | 0 | 1 | 157 | 87 | 243 | 3.6e-69 | 270.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050303 | MS.gene62076 | 0.821397 | 4.13E-53 | -1.69E-46 |
| MS.gene054657 | MS.gene62076 | 0.80573 | 1.16E-49 | -1.69E-46 |
| MS.gene055357 | MS.gene62076 | 0.811768 | 5.93E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene62076 | 0.813758 | 2.18E-51 | -1.69E-46 |
| MS.gene057060 | MS.gene62076 | 0.808532 | 2.96E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene62076 | 0.813662 | 2.29E-51 | -1.69E-46 |
| MS.gene060322 | MS.gene62076 | 0.806689 | 7.28E-50 | -1.69E-46 |
| MS.gene060429 | MS.gene62076 | 0.802248 | 6.14E-49 | -1.69E-46 |
| MS.gene060572 | MS.gene62076 | 0.8005 | 1.40E-48 | -1.69E-46 |
| MS.gene061040 | MS.gene62076 | -0.806365 | 8.52E-50 | -1.69E-46 |
| MS.gene061114 | MS.gene62076 | -0.800208 | 1.61E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62076.t1 | MTR_4g131910 | 88.535 | 157 | 18 | 0 | 1 | 157 | 87 | 243 | 3.34e-94 | 274 |
| MS.gene62076.t1 | MTR_4g119422 | 39.726 | 219 | 91 | 6 | 1 | 180 | 151 | 367 | 9.23e-34 | 123 |
| MS.gene62076.t1 | MTR_5g022730 | 36.806 | 144 | 69 | 3 | 47 | 178 | 4 | 137 | 1.37e-18 | 79.0 |
| MS.gene62076.t1 | MTR_7g021400 | 39.130 | 138 | 74 | 2 | 47 | 178 | 5 | 138 | 1.97e-18 | 78.6 |
| MS.gene62076.t1 | MTR_7g094150 | 39.130 | 138 | 74 | 2 | 47 | 178 | 5 | 138 | 3.91e-18 | 77.8 |
| MS.gene62076.t1 | MTR_5g022710 | 36.111 | 144 | 70 | 3 | 47 | 178 | 4 | 137 | 4.12e-18 | 77.4 |
| MS.gene62076.t1 | MTR_4g033455 | 33.557 | 149 | 83 | 3 | 47 | 183 | 9 | 153 | 5.25e-18 | 77.4 |
| MS.gene62076.t1 | MTR_5g085030 | 34.783 | 138 | 81 | 2 | 47 | 178 | 4 | 138 | 2.08e-17 | 75.9 |
| MS.gene62076.t1 | MTR_4g032510 | 38.971 | 136 | 72 | 3 | 47 | 178 | 6 | 134 | 2.43e-17 | 75.5 |
| MS.gene62076.t1 | MTR_7g094170 | 36.111 | 144 | 70 | 3 | 47 | 178 | 5 | 138 | 5.98e-17 | 74.7 |
| MS.gene62076.t1 | MTR_4g032460 | 35.606 | 132 | 82 | 1 | 47 | 178 | 4 | 132 | 8.58e-17 | 73.9 |
| MS.gene62076.t1 | MTR_4g032500 | 40.000 | 130 | 67 | 3 | 48 | 173 | 7 | 129 | 1.27e-16 | 73.6 |
| MS.gene62076.t1 | MTR_4g033460 | 33.333 | 138 | 82 | 2 | 47 | 178 | 9 | 142 | 2.19e-16 | 75.1 |
| MS.gene62076.t1 | MTR_4g032400 | 35.338 | 133 | 77 | 2 | 47 | 173 | 11 | 140 | 5.54e-16 | 72.0 |
| MS.gene62076.t1 | MTR_3g110048 | 35.000 | 140 | 79 | 2 | 47 | 178 | 5 | 140 | 1.55e-15 | 70.9 |
| MS.gene62076.t1 | MTR_7g094280 | 35.616 | 146 | 71 | 4 | 48 | 180 | 7 | 142 | 4.24e-15 | 69.7 |
| MS.gene62076.t1 | MTR_7g094120 | 35.211 | 142 | 76 | 4 | 47 | 178 | 6 | 141 | 4.56e-15 | 69.7 |
| MS.gene62076.t1 | MTR_8g056060 | 35.862 | 145 | 70 | 3 | 47 | 178 | 5 | 139 | 9.01e-15 | 68.9 |
| MS.gene62076.t1 | MTR_8g468480 | 33.333 | 135 | 77 | 3 | 47 | 173 | 4 | 133 | 2.36e-14 | 67.8 |
| MS.gene62076.t1 | MTR_7g021380 | 36.232 | 138 | 78 | 2 | 47 | 178 | 5 | 138 | 3.47e-14 | 67.4 |
| MS.gene62076.t1 | MTR_4g032450 | 32.484 | 157 | 76 | 4 | 34 | 168 | 14 | 162 | 6.46e-13 | 64.3 |
| MS.gene62076.t1 | MTR_4g131980 | 39.091 | 110 | 61 | 3 | 47 | 153 | 4 | 110 | 3.65e-11 | 58.9 |
| MS.gene62076.t1 | MTR_4g032420 | 35.200 | 125 | 70 | 3 | 64 | 186 | 25 | 140 | 5.60e-11 | 58.5 |
| MS.gene62076.t1 | MTR_7g094310 | 33.333 | 138 | 71 | 3 | 48 | 178 | 7 | 130 | 5.86e-11 | 58.5 |
| MS.gene62076.t1 | MTR_1g099870 | 40.860 | 93 | 47 | 1 | 102 | 186 | 1 | 93 | 6.85e-11 | 57.0 |
| MS.gene62076.t1 | MTR_4g021390 | 32.331 | 133 | 77 | 4 | 47 | 173 | 7 | 132 | 8.97e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62076.t1 | AT5G42190 | 33.333 | 159 | 68 | 4 | 48 | 178 | 5 | 153 | 1.15e-14 | 68.9 |
| MS.gene62076.t1 | AT4G34210 | 33.824 | 136 | 82 | 2 | 47 | 178 | 3 | 134 | 2.15e-14 | 67.8 |
| MS.gene62076.t1 | AT1G75950 | 33.333 | 150 | 72 | 4 | 47 | 178 | 3 | 142 | 5.29e-14 | 67.0 |
| MS.gene62076.t1 | AT3G21850 | 35.294 | 136 | 81 | 3 | 47 | 178 | 3 | 135 | 3.50e-13 | 64.7 |
| MS.gene62076.t1 | AT4G34470 | 33.088 | 136 | 83 | 2 | 47 | 178 | 3 | 134 | 4.36e-13 | 64.3 |
| MS.gene62076.t1 | AT2G03170 | 36.090 | 133 | 80 | 2 | 47 | 178 | 3 | 131 | 4.55e-13 | 64.3 |
| MS.gene62076.t1 | AT3G21860 | 34.507 | 142 | 73 | 4 | 47 | 178 | 3 | 134 | 1.11e-11 | 60.5 |
| MS.gene62076.t1 | AT3G60020 | 31.655 | 139 | 85 | 3 | 46 | 178 | 1 | 135 | 1.75e-11 | 60.1 |
Find 40 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGCATGGGATAAGAAATT+TGG | 0.172220 | 8.2:-2901077 | MS.gene62076:CDS |
| GTAATTGCAGGAAGAGAAAA+TGG | 0.299795 | 8.2:-2901435 | MS.gene62076:intron |
| CTTCTTGCGCGGCATAGAAT+TGG | 0.314810 | 8.2:+2901021 | None:intergenic |
| ATCTTCTCTTCTGCCCGTTT+CGG | 0.316377 | 8.2:+2901878 | None:intergenic |
| GGTTGTGTTTGAAGTTGATT+TGG | 0.328338 | 8.2:-2901360 | MS.gene62076:CDS |
| TGAAGATCATCCAGAGTTAT+AGG | 0.337687 | 8.2:+2901140 | None:intergenic |
| ACTTTCTTTGATAAAACATC+TGG | 0.378071 | 8.2:+2901404 | None:intergenic |
| AACACTTGCAATTAAAGCAT+TGG | 0.403799 | 8.2:-2901849 | MS.gene62076:CDS |
| GTTGTGTTTGAAGTTGATTT+GGG | 0.419950 | 8.2:-2901359 | MS.gene62076:CDS |
| GTTTGAAGTTGATTTGGGTT+TGG | 0.429981 | 8.2:-2901354 | MS.gene62076:CDS |
| GATGATCTTCATACATATGC+AGG | 0.437688 | 8.2:-2901128 | MS.gene62076:CDS |
| ATTCACTTCATGAACAGAAA+TGG | 0.455202 | 8.2:+2901265 | None:intergenic |
| AGAGACTTGGGAACCTTGCA+TGG | 0.478969 | 8.2:-2901091 | MS.gene62076:CDS |
| TGATGTTGACCCTATAACTC+TGG | 0.483537 | 8.2:-2901150 | MS.gene62076:CDS |
| TATCCAGATGGATCCGAAAC+GGG | 0.489766 | 8.2:-2901891 | None:intergenic |
| TAGAATTGGACGAGTTCCTC+TGG | 0.497801 | 8.2:+2901035 | None:intergenic |
| TCAACATCACCAATCGGAAT+CGG | 0.502713 | 8.2:+2901290 | None:intergenic |
| GAAAGTCAACTTGGTAAGCT+CGG | 0.520925 | 8.2:-2901387 | MS.gene62076:CDS |
| CAAATTTCTTATCCCATGCA+AGG | 0.522321 | 8.2:+2901078 | None:intergenic |
| TCAACTTGGTAAGCTCGGAC+AGG | 0.523618 | 8.2:-2901382 | MS.gene62076:CDS |
| GTATCCAGATGGATCCGAAA+CGG | 0.528518 | 8.2:-2901892 | None:intergenic |
| AGTATGTTGATCTCAAGGAC+TGG | 0.535333 | 8.2:-2901187 | MS.gene62076:CDS |
| GAAGATCATCCAGAGTTATA+GGG | 0.538617 | 8.2:+2901141 | None:intergenic |
| GAGACCATTCCGATTCCGAT+TGG | 0.541494 | 8.2:-2901299 | MS.gene62076:CDS |
| ATGGTATCAACATCACCAAT+CGG | 0.541955 | 8.2:+2901284 | None:intergenic |
| TTTATCAAAGAAAGTCAACT+TGG | 0.543241 | 8.2:-2901396 | MS.gene62076:CDS |
| GTATGTTGATCTCAAGGACT+GGG | 0.555633 | 8.2:-2901186 | MS.gene62076:CDS |
| ACAGAAGTATGTTGATCTCA+AGG | 0.556799 | 8.2:-2901192 | MS.gene62076:CDS |
| ATCACCAATCGGAATCGGAA+TGG | 0.586243 | 8.2:+2901295 | None:intergenic |
| TACTTGAAAATCGAGAGACT+TGG | 0.593142 | 8.2:-2901104 | MS.gene62076:CDS |
| CAACTTGGTAAGCTCGGACA+GGG | 0.603846 | 8.2:-2901381 | MS.gene62076:CDS |
| CTCAAGAATACACAAGGCAA+AGG | 0.606376 | 8.2:-2901801 | MS.gene62076:intron |
| GAAGTTTGAGTCTTCTTGCG+CGG | 0.607395 | 8.2:+2901010 | None:intergenic |
| ACTTGAAAATCGAGAGACTT+GGG | 0.609483 | 8.2:-2901103 | MS.gene62076:CDS |
| GAATAGCAAAATGTTGAGCA+TGG | 0.627648 | 8.2:-2901246 | MS.gene62076:CDS |
| GAGACTTGGGAACCTTGCAT+GGG | 0.636934 | 8.2:-2901090 | MS.gene62076:CDS |
| AAATCTAAAGACTCTTCTCG+TGG | 0.650288 | 8.2:+2900970 | None:intergenic |
| ATGATCTTCATACATATGCA+GGG | 0.656987 | 8.2:-2901127 | MS.gene62076:CDS |
| TGATTGCTCAAGAATACACA+AGG | 0.692172 | 8.2:-2901807 | MS.gene62076:CDS |
| GATAAAAGACATGACACCAG+AGG | 0.694992 | 8.2:-2901051 | MS.gene62076:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGATTTATATATGACTATTT+CGG | - | chr8.2:2901325-2901344 | MS.gene62076:CDS | 15.0% |
| !!! | AATTCTTTTGAAATATGTTT+TGG | - | chr8.2:2901142-2901161 | MS.gene62076:CDS | 15.0% |
| !! | AAAGAAATATTAGGATTCTT+TGG | - | chr8.2:2901298-2901317 | MS.gene62076:CDS | 20.0% |
| !! | ATCCTAATATTTCTTTATAG+TGG | + | chr8.2:2901294-2901313 | None:intergenic | 20.0% |
| !! | GATTTATATATGACTATTTC+GGG | - | chr8.2:2901326-2901345 | MS.gene62076:CDS | 20.0% |
| !! | TACCACTATAAAGAAATATT+AGG | - | chr8.2:2901289-2901308 | MS.gene62076:CDS | 20.0% |
| !!! | TTAGTCTAGTTTTTTTAACT+TGG | + | chr8.2:2901393-2901412 | None:intergenic | 20.0% |
| ! | AAACTAGACTAAGATATTAG+TGG | - | chr8.2:2901401-2901420 | MS.gene62076:CDS | 25.0% |
| ! | ACTTATGTCAAAAAGATTTG+AGG | - | chr8.2:2901528-2901547 | MS.gene62076:intron | 25.0% |
| ! | ACTTTCTTTGATAAAACATC+TGG | + | chr8.2:2901457-2901476 | None:intergenic | 25.0% |
| ! | TAAAATTTCCATATCTAGCA+TGG | - | chr8.2:2901119-2901138 | MS.gene62076:CDS | 25.0% |
| ! | TTATGTATAAGACTCTAGTT+TGG | + | chr8.2:2901227-2901246 | None:intergenic | 25.0% |
| ! | TTTATCAAAGAAAGTCAACT+TGG | - | chr8.2:2901462-2901481 | MS.gene62076:intron | 25.0% |
| !!! | AACAGTTTTAAATCTTGAGA+AGG | - | chr8.2:2901261-2901280 | MS.gene62076:CDS | 25.0% |
| !!! | CTTTTGAAATATGTTTTGGT+CGG | - | chr8.2:2901146-2901165 | MS.gene62076:CDS | 25.0% |
| AAAGAATTCCATGCTAGATA+TGG | + | chr8.2:2901130-2901149 | None:intergenic | 30.0% | |
| AACACTTGCAATTAAAGCAT+TGG | - | chr8.2:2901009-2901028 | MS.gene62076:CDS | 30.0% | |
| AAGATATTAGTGGTAATTGC+AGG | - | chr8.2:2901411-2901430 | MS.gene62076:CDS | 30.0% | |
| ATGATCTTCATACATATGCA+GGG | - | chr8.2:2901731-2901750 | MS.gene62076:intron | 30.0% | |
| ATTCACTTCATGAACAGAAA+TGG | + | chr8.2:2901596-2901615 | None:intergenic | 30.0% | |
| TCGTTAGGAAAATTGTATTC+AGG | - | chr8.2:2901178-2901197 | MS.gene62076:CDS | 30.0% | |
| ! | GTTGTGTTTGAAGTTGATTT+GGG | - | chr8.2:2901499-2901518 | MS.gene62076:intron | 30.0% |
| ACTTGAAAATCGAGAGACTT+GGG | - | chr8.2:2901755-2901774 | MS.gene62076:intron | 35.0% | |
| ATGGTATCAACATCACCAAT+CGG | + | chr8.2:2901577-2901596 | None:intergenic | 35.0% | |
| CAAATTTCTTATCCCATGCA+AGG | + | chr8.2:2901783-2901802 | None:intergenic | 35.0% | |
| CTTGCATGGGATAAGAAATT+TGG | - | chr8.2:2901781-2901800 | MS.gene62076:intron | 35.0% | |
| GAAGATCATCCAGAGTTATA+GGG | + | chr8.2:2901720-2901739 | None:intergenic | 35.0% | |
| GAATAGCAAAATGTTGAGCA+TGG | - | chr8.2:2901612-2901631 | MS.gene62076:intron | 35.0% | |
| GATGATCTTCATACATATGC+AGG | - | chr8.2:2901730-2901749 | MS.gene62076:intron | 35.0% | |
| GTAATTGCAGGAAGAGAAAA+TGG | - | chr8.2:2901423-2901442 | MS.gene62076:CDS | 35.0% | |
| TACTTGAAAATCGAGAGACT+TGG | - | chr8.2:2901754-2901773 | MS.gene62076:intron | 35.0% | |
| TGAAGATCATCCAGAGTTAT+AGG | + | chr8.2:2901721-2901740 | None:intergenic | 35.0% | |
| TGATTGCTCAAGAATACACA+AGG | - | chr8.2:2901051-2901070 | MS.gene62076:CDS | 35.0% | |
| ! | ACAGAAGTATGTTGATCTCA+AGG | - | chr8.2:2901666-2901685 | MS.gene62076:intron | 35.0% |
| ! | GGTTGTGTTTGAAGTTGATT+TGG | - | chr8.2:2901498-2901517 | MS.gene62076:intron | 35.0% |
| !! | GTTTGAAGTTGATTTGGGTT+TGG | - | chr8.2:2901504-2901523 | MS.gene62076:intron | 35.0% |
| AGTATGTTGATCTCAAGGAC+TGG | - | chr8.2:2901671-2901690 | MS.gene62076:intron | 40.0% | |
| CTCAAGAATACACAAGGCAA+AGG | - | chr8.2:2901057-2901076 | MS.gene62076:CDS | 40.0% | |
| GAAAGTCAACTTGGTAAGCT+CGG | - | chr8.2:2901471-2901490 | MS.gene62076:intron | 40.0% | |
| GATAAAAGACATGACACCAG+AGG | - | chr8.2:2901807-2901826 | MS.gene62076:CDS | 40.0% | |
| GTATGTTGATCTCAAGGACT+GGG | - | chr8.2:2901672-2901691 | MS.gene62076:intron | 40.0% | |
| TCAACATCACCAATCGGAAT+CGG | + | chr8.2:2901571-2901590 | None:intergenic | 40.0% | |
| TGATGTTGACCCTATAACTC+TGG | - | chr8.2:2901708-2901727 | MS.gene62076:intron | 40.0% | |
| ATCACCAATCGGAATCGGAA+TGG | + | chr8.2:2901566-2901585 | None:intergenic | 45.0% | |
| ATCTTCTCTTCTGCCCGTTT+CGG | + | chr8.2:2900983-2901002 | None:intergenic | 45.0% | |
| GAAGTTTGAGTCTTCTTGCG+CGG | + | chr8.2:2901851-2901870 | None:intergenic | 45.0% | |
| TAGAATTGGACGAGTTCCTC+TGG | + | chr8.2:2901826-2901845 | None:intergenic | 45.0% | |
| ! | AATTTTCCTAACGACGCACG+AGG | + | chr8.2:2901172-2901191 | None:intergenic | 45.0% |
| AGAGACTTGGGAACCTTGCA+TGG | - | chr8.2:2901767-2901786 | MS.gene62076:intron | 50.0% | |
| CAACTTGGTAAGCTCGGACA+GGG | - | chr8.2:2901477-2901496 | MS.gene62076:intron | 50.0% | |
| CTTCTTGCGCGGCATAGAAT+TGG | + | chr8.2:2901840-2901859 | None:intergenic | 50.0% | |
| GAGACCATTCCGATTCCGAT+TGG | - | chr8.2:2901559-2901578 | MS.gene62076:intron | 50.0% | |
| GAGACTTGGGAACCTTGCAT+GGG | - | chr8.2:2901768-2901787 | MS.gene62076:intron | 50.0% | |
| TCAACTTGGTAAGCTCGGAC+AGG | - | chr8.2:2901476-2901495 | MS.gene62076:intron | 50.0% | |
| GGTCGGCCTCGTGCGTCGTT+AGG | - | chr8.2:2901163-2901182 | MS.gene62076:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 2900974 | 2901906 | 2900974 | ID=MS.gene62076 |
| chr8.2 | mRNA | 2900974 | 2901906 | 2900974 | ID=MS.gene62076.t1;Parent=MS.gene62076 |
| chr8.2 | exon | 2901802 | 2901906 | 2901802 | ID=MS.gene62076.t1.exon1;Parent=MS.gene62076.t1 |
| chr8.2 | CDS | 2901802 | 2901906 | 2901802 | ID=cds.MS.gene62076.t1;Parent=MS.gene62076.t1 |
| chr8.2 | exon | 2900974 | 2901447 | 2900974 | ID=MS.gene62076.t1.exon2;Parent=MS.gene62076.t1 |
| chr8.2 | CDS | 2900974 | 2901447 | 2900974 | ID=cds.MS.gene62076.t1;Parent=MS.gene62076.t1 |
| Gene Sequence |
| Protein sequence |
