AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62500.t1 MTR_2g064405 98.473 393 4 2 1 391 1 393 0.0 791
MS.gene62500.t1 MTR_2g064405 99.684 316 1 0 76 391 1 316 0.0 654
MS.gene62500.t1 MTR_8g046550 78.372 393 76 3 2 391 8 394 0.0 613
MS.gene62500.t1 MTR_3g080090 65.823 395 122 2 2 388 7 396 0.0 523
MS.gene62500.t1 MTR_7g022890 61.942 381 136 5 12 387 19 395 5.67e-160 455
MS.gene62500.t1 MTR_7g022890 65.569 334 113 2 55 387 10 342 3.43e-156 444
MS.gene62500.t1 MTR_3g062610 56.707 328 135 4 61 387 72 393 2.79e-127 372
MS.gene62500.t1 MTR_5g075680 54.268 328 143 4 61 387 12 333 3.54e-120 352
MS.gene62500.t1 MTR_5g075680 52.890 346 151 5 44 388 53 387 5.62e-120 353
MS.gene62500.t1 MTR_3g062610 52.564 234 105 3 61 293 72 300 1.08e-79 248
MS.gene62500.t1 MTR_5g075680 46.067 267 132 5 44 309 53 308 2.56e-71 226
MS.gene62500.t1 MTR_5g075680 42.289 201 105 4 44 243 53 243 1.07e-42 150
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62500.t1 AT1G16310 75.806 372 88 1 19 390 59 428 0.0 576
MS.gene62500.t1 AT1G79520 71.654 381 103 2 14 391 24 402 0.0 543
MS.gene62500.t1 AT1G79520 69.466 393 103 3 14 391 24 414 0.0 534
MS.gene62500.t1 AT1G79520 76.471 340 80 0 52 391 10 349 0.0 534
MS.gene62500.t1 AT1G79520 76.471 340 80 0 52 391 10 349 0.0 534
MS.gene62500.t1 AT1G16310 79.048 315 66 0 76 390 1 315 0.0 509
MS.gene62500.t1 AT2G39450 58.673 392 155 4 2 390 5 392 5.47e-140 404
MS.gene62500.t1 AT3G58060 54.381 331 147 3 61 387 71 401 9.76e-122 358
MS.gene62500.t1 AT3G58060 54.381 331 147 3 61 387 71 401 9.76e-122 358

Find 67 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAAGTTAACTTACCTTATA+TGG 0.125547 2.1:+31271685 MS.gene62500:CDS
ATCATCCAATTCACCTTTAC+TGG 0.222158 2.1:-31270672 None:intergenic
GTTCATGGTGGTTCCATATA+AGG 0.230476 2.1:-31271698 None:intergenic
ACAAAAGCTCTTTCAACTTC+TGG 0.237828 2.1:-31271864 None:intergenic
TTCTAGCTGTCAAGTTCTTC+TGG 0.275479 2.1:+31270835 MS.gene62500:CDS
ATGAAGTTTCAAACGGTTCT+TGG 0.284276 2.1:+31267931 MS.gene62500:CDS
AACAGCACCACCTGAATTCT+TGG 0.293127 2.1:+31271662 MS.gene62500:CDS
CTATGTGCTTGATTTGTTCA+TGG 0.301014 2.1:-31271713 None:intergenic
GTTAACTTCGCCAAGAATTC+AGG 0.307867 2.1:-31271672 None:intergenic
GAATTGGATGATCGGGATTA+TGG 0.321624 2.1:+31270683 MS.gene62500:CDS
TTCCAATCGGATAGTGATAT+CGG 0.334945 2.1:-31270294 None:intergenic
ACCTTTACTGGATCGACTTC+TGG 0.344775 2.1:-31270660 None:intergenic
CTCTTGTTTCTTGTAATACT+CGG 0.363210 2.1:-31269604 None:intergenic
AAATCTAAGTTTAGTTTCAA+TGG 0.375459 2.1:+31268011 MS.gene62500:CDS
ATCAACACTAGACTCTCTTT+TGG 0.380655 2.1:+31270216 MS.gene62500:CDS
TCGATCCAGTAAAGGTGAAT+TGG 0.389302 2.1:+31270667 MS.gene62500:CDS
TGCATCCGTGATGGCAACCT+TGG 0.401323 2.1:+31270469 MS.gene62500:CDS
CTTGGTGTTGTTTGCAGCAA+AGG 0.416822 2.1:+31270153 MS.gene62500:CDS
GAGAATGTTTGGTCACTCAT+TGG 0.417814 2.1:+31271636 MS.gene62500:CDS
TCTTGTCTGGTTTCATATTG+TGG 0.420819 2.1:+31270242 MS.gene62500:CDS
AGACAGTGATGGAGAATGTT+TGG 0.426193 2.1:+31271625 MS.gene62500:CDS
CCTCGAAGGATTTAATGAAA+TGG 0.427590 2.1:+31269632 MS.gene62500:CDS
ATCACCTCAGAGAGACCTCC+TGG 0.432671 2.1:-31269681 None:intergenic
ACAGTTAGAGCATACACATT+TGG 0.444981 2.1:+31271738 MS.gene62500:CDS
CCATTTCATTAAATCCTTCG+AGG 0.445178 2.1:-31269632 None:intergenic
CTTCTTGATGAAGTTTCAAA+CGG 0.448380 2.1:+31267924 MS.gene62500:CDS
GCATCCGTGATGGCAACCTT+GGG 0.449922 2.1:+31270470 MS.gene62500:CDS
TGGTTGAGAAACATGTCTTC+TGG 0.452682 2.1:-31271795 None:intergenic
ATGGAAGCTATGCATGAAAC+TGG 0.456771 2.1:+31269651 MS.gene62500:CDS
TTTCGCCGATGTTGTGTGCT+TGG 0.461751 2.1:-31271815 None:intergenic
ATCGGATAGTGATATCGGTT+TGG 0.466363 2.1:-31270289 None:intergenic
TAAAGGTGAATTGGATGATC+GGG 0.471055 2.1:+31270676 MS.gene62500:CDS
TGGAAAGAAACGCATGCAAC+CGG 0.477866 2.1:+31270312 MS.gene62500:CDS
TGCATGCGTTTCTTTCCAAT+CGG 0.484472 2.1:-31270307 None:intergenic
CATACTGATGTCTTATGCTC+AGG 0.504545 2.1:-31271915 None:intergenic
TTCTGGTGGATGGATCCAAC+AGG 0.504950 2.1:+31270852 MS.gene62500:CDS
AACCGATATCACTATCCGAT+TGG 0.515935 2.1:+31270292 MS.gene62500:CDS
ATATCGGTTTGGCTTGCTCA+TGG 0.516883 2.1:-31270278 None:intergenic
ATTTGGTGCAGATTACTTTG+TGG 0.527294 2.1:+31271755 MS.gene62500:CDS
CAAGCCCAAGGTTGCCATCA+CGG 0.531642 2.1:-31270474 None:intergenic
TGTCAAGTTCTTCTGGTGGA+TGG 0.542414 2.1:+31270842 MS.gene62500:CDS
TTTGAAACTTCATCAAGAAG+AGG 0.542941 2.1:-31267921 None:intergenic
TAATGATCTGCTCTCGATTG+AGG 0.549611 2.1:-31270182 None:intergenic
CTTACAAGAATAGCTCCTGT+TGG 0.551242 2.1:-31270867 None:intergenic
GCCAGAAGTCGATCCAGTAA+AGG 0.564036 2.1:+31270659 MS.gene62500:CDS
TAGCTGTCAAGTTCTTCTGG+TGG 0.564250 2.1:+31270838 MS.gene62500:CDS
GTAAAGGTGAATTGGATGAT+CGG 0.574495 2.1:+31270675 MS.gene62500:CDS
CACTATATACAATCAACACA+TGG 0.581728 2.1:+31271598 MS.gene62500:CDS
ACTTGCAAAGAGTGAGAGGA+CGG 0.587244 2.1:+31270105 MS.gene62500:CDS
TGTGTCAAATGCATGTAACT+TGG 0.588818 2.1:+31270135 MS.gene62500:CDS
GATGTCATTACTAATTCCGT+CGG 0.610281 2.1:+31270798 MS.gene62500:CDS
TGATCAACAACATTCTGTGA+TGG 0.612919 2.1:-31267981 None:intergenic
AGCAACTTGCAAAGAGTGAG+AGG 0.619088 2.1:+31270101 MS.gene62500:CDS
GATCAAAATCTGCAAGCCCA+AGG 0.622295 2.1:-31270486 None:intergenic
AACAGCGGCGGCTAATCCGA+CGG 0.625123 2.1:-31270814 None:intergenic
GAACTTGACAGCTAGAACAG+CGG 0.628290 2.1:-31270829 None:intergenic
AAACAAGAGAGACTCCTCGA+AGG 0.629592 2.1:+31269618 MS.gene62500:CDS
CTCAACCAAGCACACAACAT+CGG 0.630772 2.1:+31271810 MS.gene62500:CDS
TCACCTCAGAGAGACCTCCT+GGG 0.631079 2.1:-31269680 None:intergenic
AACTTCGCCAAGAATTCAGG+TGG 0.631351 2.1:-31271669 None:intergenic
TGTGCTTGATTTGTTCATGG+TGG 0.640773 2.1:-31271710 None:intergenic
TTTCCCAGGAGGTCTCTCTG+AGG 0.647360 2.1:+31269677 MS.gene62500:CDS
CACATGGACAAAGACAGTGA+TGG 0.674860 2.1:+31271614 MS.gene62500:CDS
AAAGAAACGCATGCAACCGG+TGG 0.685895 2.1:+31270315 MS.gene62500:CDS
TGTTTCAGGGAAACAACGCA+AGG 0.686235 2.1:+31269578 MS.gene62500:intron
TTCAGGGAAACAACGCAAGG+TGG 0.700523 2.1:+31269581 MS.gene62500:intron
CTTGACAGCTAGAACAGCGG+CGG 0.725540 2.1:-31270826 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAAAATATTATAAATT+AGG - chr2.1:31268920-31268939 None:intergenic 0.0%
!!! AATTTATAATATTTTTTTAA+AGG + chr2.1:31268920-31268939 MS.gene62500:intron 0.0%
!!! AGTTTTATTGTAATAATAAT+GGG + chr2.1:31268634-31268653 MS.gene62500:intron 10.0%
!!! TAGTTTTATTGTAATAATAA+TGG + chr2.1:31268633-31268652 MS.gene62500:intron 10.0%
!!! TATTATCAATTTTTTTTTTC+AGG + chr2.1:31269325-31269344 MS.gene62500:intron 10.0%
!! AAACAGAAAAAGAAAAAAAT+AGG - chr2.1:31268274-31268293 None:intergenic 15.0%
!!! ACTTACAATTTTCTTATAAA+CGG + chr2.1:31269726-31269745 MS.gene62500:intron 15.0%
!!! GTTTTATTGTAATAATAATG+GGG + chr2.1:31268635-31268654 MS.gene62500:intron 15.0%
!!! TTCTAAAGTTTATGTTTTTT+TGG + chr2.1:31268073-31268092 MS.gene62500:intron 15.0%
!! AAGAAAAAAATAGGTGAAAA+AGG - chr2.1:31268265-31268284 None:intergenic 20.0%
!! ACCATACAAGAAAATTTAAA+AGG - chr2.1:31268522-31268541 None:intergenic 20.0%
!! AGTTATGGGAAATTTATTAA+TGG - chr2.1:31270972-31270991 None:intergenic 20.0%
!! AGTTTATGTTTAATATGTGT+GGG - chr2.1:31268458-31268477 None:intergenic 20.0%
!! CATGTGTATGTTTAATATTA+AGG + chr2.1:31268334-31268353 MS.gene62500:intron 20.0%
!! GCAACAACAAAATAAAATAA+TGG - chr2.1:31268810-31268829 None:intergenic 20.0%
!! TAATGGAAAAATACTAATTG+TGG - chr2.1:31270955-31270974 None:intergenic 20.0%
!! TAGTTTATGTTTAATATGTG+TGG - chr2.1:31268459-31268478 None:intergenic 20.0%
!! TTAAACAAACCGTTATTATT+CGG - chr2.1:31269923-31269942 None:intergenic 20.0%
!! TTAACTTTATTATGTGAACA+AGG - chr2.1:31268996-31269015 None:intergenic 20.0%
!!! AAATCTAAGTTTAGTTTCAA+TGG + chr2.1:31268011-31268030 MS.gene62500:CDS 20.0%
!!! AATTTTTTTTTTCAGGTGAA+GGG + chr2.1:31269332-31269351 MS.gene62500:intron 20.0%
!!! TATCATGATCTTACTTTTTA+AGG - chr2.1:31268597-31268616 None:intergenic 20.0%
!!! TATGAAAATTCTGAAATTTC+TGG + chr2.1:31269050-31269069 MS.gene62500:intron 20.0%
!!! TCCTTTTAAATTTTCTTGTA+TGG + chr2.1:31268518-31268537 MS.gene62500:intron 20.0%
!!! TTATTTTTCAACTTGCTTTT+AGG + chr2.1:31269375-31269394 MS.gene62500:intron 20.0%
!!! TTTAATTAGTTCAACTGTTT+TGG + chr2.1:31271269-31271288 MS.gene62500:intron 20.0%
! AAAAGCAAAGAACAACATAA+TGG - chr2.1:31268122-31268141 None:intergenic 25.0%
! AAAATAACTGACCATAGTTA+AGG + chr2.1:31271235-31271254 MS.gene62500:intron 25.0%
! AAAATTGCATTATGTTTGTC+AGG + chr2.1:31270069-31270088 MS.gene62500:intron 25.0%
! AAAGCAAAGAACAACATAAT+GGG - chr2.1:31268121-31268140 None:intergenic 25.0%
! AAATTTAAGAATGACTCACT+AGG - chr2.1:31268050-31268069 None:intergenic 25.0%
! AATCACCAATGTTATCAAAT+AGG - chr2.1:31269974-31269993 None:intergenic 25.0%
! AGACTTTGTAGTAAAAATCA+TGG - chr2.1:31268392-31268411 None:intergenic 25.0%
! ATCACCAATGTTATCAAATA+GGG - chr2.1:31269973-31269992 None:intergenic 25.0%
! GAACTAATTAAACCTTAAAC+AGG - chr2.1:31271261-31271280 None:intergenic 25.0%
! GACAAAATAATGATGTGTAA+AGG - chr2.1:31268892-31268911 None:intergenic 25.0%
! GTGTATGTTTAATATTAAGG+TGG + chr2.1:31268337-31268356 MS.gene62500:intron 25.0%
! TAATGGGGATCTTTAATTAA+AGG + chr2.1:31268650-31268669 MS.gene62500:intron 25.0%
! TCAAAAGCTACAAATTCTAT+TGG - chr2.1:31269289-31269308 None:intergenic 25.0%
! TGATTACCTTTGCAAAAATT+TGG - chr2.1:31270523-31270542 None:intergenic 25.0%
! TTATTATGTGAACAAGGTAA+TGG - chr2.1:31268990-31269009 None:intergenic 25.0%
!! AAAACTGATTTAAAGATGCT+TGG + chr2.1:31269223-31269242 MS.gene62500:intron 25.0%
!! AAAGTTTCAATTTTGTGTGA+TGG + chr2.1:31268169-31268188 MS.gene62500:intron 25.0%
!! AAGTTTCAATTTTGTGTGAT+GGG + chr2.1:31268170-31268189 MS.gene62500:intron 25.0%
!! ACACATACTAGTTTATTAAG+TGG + chr2.1:31269495-31269514 MS.gene62500:intron 25.0%
!! AGTATCATTCTTTTTCTTAC+AGG + chr2.1:31270632-31270651 MS.gene62500:intron 25.0%
!! TCTATTGGAATATTTTCTAG+TGG - chr2.1:31269274-31269293 None:intergenic 25.0%
!! TTAACTATGGTCAGTTATTT+TGG - chr2.1:31271236-31271255 None:intergenic 25.0%
!! TTTGTAGCTTTTGACAATTA+AGG + chr2.1:31269295-31269314 MS.gene62500:intron 25.0%
!!! ATTTTTAAAGCATTGCTTCT+AGG - chr2.1:31271123-31271142 None:intergenic 25.0%
!!! CAATTTTTTTTTTCAGGTGA+AGG + chr2.1:31269331-31269350 MS.gene62500:intron 25.0%
!!! CACTTTTTTTATATGATCGT+TGG + chr2.1:31271504-31271523 MS.gene62500:intron 25.0%
!!! CTTTTAGATTCTCTGTTTTT+TGG + chr2.1:31268547-31268566 MS.gene62500:intron 25.0%
AATTAAAGGAAGAGCCTTTA+TGG + chr2.1:31268664-31268683 MS.gene62500:intron 30.0%
ACACGATTTAGTATAAAGTG+TGG - chr2.1:31269896-31269915 None:intergenic 30.0%
AGGTGAAAAAGGATTAACAA+TGG - chr2.1:31268254-31268273 None:intergenic 30.0%
CACTATATACAATCAACACA+TGG + chr2.1:31271598-31271617 MS.gene62500:CDS 30.0%
CATCATTAGTACATCAATCA+TGG - chr2.1:31271546-31271565 None:intergenic 30.0%
CATGACTTAAAGTGTATGTT+TGG + chr2.1:31269163-31269182 MS.gene62500:intron 30.0%
CGAAGTTAACTTACCTTATA+TGG + chr2.1:31271685-31271704 MS.gene62500:CDS 30.0%
CTTCTTGATGAAGTTTCAAA+CGG + chr2.1:31267924-31267943 MS.gene62500:CDS 30.0%
TACATGTGTTTGAATCGATT+AGG - chr2.1:31270007-31270026 None:intergenic 30.0%
TATAAAGTGTGGTCAAATAG+TGG - chr2.1:31269885-31269904 None:intergenic 30.0%
TCACCAATGTTATCAAATAG+GGG - chr2.1:31269972-31269991 None:intergenic 30.0%
TGTGTAAAGGAATTAAGTGA+AGG - chr2.1:31268879-31268898 None:intergenic 30.0%
TTGAATCGATTAGGTTCAAT+TGG - chr2.1:31269998-31270017 None:intergenic 30.0%
TTGATGAATATCCACCATAA+AGG - chr2.1:31268681-31268700 None:intergenic 30.0%
! AAAGTGTATGTTTGGTTTCA+TGG + chr2.1:31269171-31269190 MS.gene62500:intron 30.0%
! AAATACTTATGTTTCTGTGC+AGG + chr2.1:31270433-31270452 MS.gene62500:intron 30.0%
! AAGCTTTGATTGTGTACAAA+TGG - chr2.1:31268839-31268858 None:intergenic 30.0%
! AATACTTATGTTTCTGTGCA+GGG + chr2.1:31270434-31270453 MS.gene62500:intron 30.0%
! ATGATTGTTTGCTAGTTAGA+GGG + chr2.1:31271060-31271079 MS.gene62500:intron 30.0%
! CTCTTGTTTCTTGTAATACT+CGG - chr2.1:31269607-31269626 None:intergenic 30.0%
! GAATGTGAAGTGTTTATGTT+TGG - chr2.1:31269417-31269436 None:intergenic 30.0%
! TTTGAAACTTCATCAAGAAG+AGG - chr2.1:31267924-31267943 None:intergenic 30.0%
!! CTAGTATGTGTTTGATTTTG+CGG - chr2.1:31269486-31269505 None:intergenic 30.0%
!! TGTACTTCTGAAGTAACTTT+GGG - chr2.1:31271438-31271457 None:intergenic 30.0%
!!! TAATTTTTTACCAGCTTCTC+AGG - chr2.1:31269087-31269106 None:intergenic 30.0%
!!! TCTAGCTTTTGGAAAAATCA+CGG + chr2.1:31268409-31268428 MS.gene62500:intron 30.0%
!!! TTACTACAAAGTCTAGCTTT+TGG + chr2.1:31268398-31268417 MS.gene62500:intron 30.0%
!!! TTAGATTCTCTGTTTTTTGG+TGG + chr2.1:31268550-31268569 MS.gene62500:intron 30.0%
!!! TTAGTTCAACTGTTTTGGTT+CGG + chr2.1:31271274-31271293 MS.gene62500:intron 30.0%
AATTTCCCATAACTCCACAA+TGG + chr2.1:31270978-31270997 MS.gene62500:intron 35.0%
ACAAAAGCTCTTTCAACTTC+TGG - chr2.1:31271867-31271886 None:intergenic 35.0%
ACAGTTAGAGCATACACATT+TGG + chr2.1:31271738-31271757 MS.gene62500:CDS 35.0%
ACATCAAAATGTTGTCGAAG+GGG + chr2.1:31271025-31271044 MS.gene62500:intron 35.0%
AGATCCTAGAAACAATCTCT+CGG + chr2.1:31271329-31271348 MS.gene62500:intron 35.0%
ATCATCCAATTCACCTTTAC+TGG - chr2.1:31270675-31270694 None:intergenic 35.0%
ATGAAGTTTCAAACGGTTCT+TGG + chr2.1:31267931-31267950 MS.gene62500:CDS 35.0%
ATTTCCCATAACTCCACAAT+GGG + chr2.1:31270979-31270998 MS.gene62500:intron 35.0%
CAATCTGCAATACACATTCA+GGG - chr2.1:31271578-31271597 None:intergenic 35.0%
CACATCAAAATGTTGTCGAA+GGG + chr2.1:31271024-31271043 MS.gene62500:intron 35.0%
CCATTTCATTAAATCCTTCG+AGG - chr2.1:31269635-31269654 None:intergenic 35.0%
CCTCGAAGGATTTAATGAAA+TGG + chr2.1:31269632-31269651 MS.gene62500:CDS 35.0%
CGTGTATTGCCGAATAATAA+CGG + chr2.1:31269911-31269930 MS.gene62500:intron 35.0%
GATGTCATTACTAATTCCGT+CGG + chr2.1:31270798-31270817 MS.gene62500:CDS 35.0%
GTAAAGGTGAATTGGATGAT+CGG + chr2.1:31270675-31270694 MS.gene62500:CDS 35.0%
TAAAGGTGAATTGGATGATC+GGG + chr2.1:31270676-31270695 MS.gene62500:CDS 35.0%
TATCAATTTACCTGAGAAGC+TGG + chr2.1:31269074-31269093 MS.gene62500:intron 35.0%
TGATCAACAACATTCTGTGA+TGG - chr2.1:31267984-31268003 None:intergenic 35.0%
TGGGGATTACTCGAAAAAAA+CGG - chr2.1:31271419-31271438 None:intergenic 35.0%
TGTGTCAAATGCATGTAACT+TGG + chr2.1:31270135-31270154 MS.gene62500:CDS 35.0%
TTCCAATCGGATAGTGATAT+CGG - chr2.1:31270297-31270316 None:intergenic 35.0%
TTTGATGTGGCTTATACGAA+AGG - chr2.1:31271013-31271032 None:intergenic 35.0%
! ATCAACACTAGACTCTCTTT+TGG + chr2.1:31270216-31270235 MS.gene62500:CDS 35.0%
! ATTTTGTTCGCATTTCAGTG+TGG + chr2.1:31270906-31270925 MS.gene62500:intron 35.0%
! CATGATTGTTTGCTAGTTAG+AGG + chr2.1:31271059-31271078 MS.gene62500:intron 35.0%
! TCTTGTCTGGTTTCATATTG+TGG + chr2.1:31270242-31270261 MS.gene62500:CDS 35.0%
! TTGCGGATTACGGAAAATAT+CGG + chr2.1:31269814-31269833 MS.gene62500:intron 35.0%
!! AAAAAAGTGCTTGTATCGTC+CGG - chr2.1:31271493-31271512 None:intergenic 35.0%
!! ATTTGGTGCAGATTACTTTG+TGG + chr2.1:31271755-31271774 MS.gene62500:CDS 35.0%
!! CAAAGAACAACATAATGGGT+CGG - chr2.1:31268117-31268136 None:intergenic 35.0%
!! CTATGTGCTTGATTTGTTCA+TGG - chr2.1:31271716-31271735 None:intergenic 35.0%
!! GTACTTCTGAAGTAACTTTG+GGG - chr2.1:31271437-31271456 None:intergenic 35.0%
!! GTGTACTTCTGAAGTAACTT+TGG - chr2.1:31271439-31271458 None:intergenic 35.0%
!!! ACTATGGTCAGTTATTTTGG+CGG - chr2.1:31271233-31271252 None:intergenic 35.0%
!!! GATTTTTAGCGTGTCAATTG+CGG + chr2.1:31269797-31269816 MS.gene62500:intron 35.0%
AAAGGAGTTTACACCCATTG+TGG - chr2.1:31270995-31271014 None:intergenic 40.0%
AACCGATATCACTATCCGAT+TGG + chr2.1:31270292-31270311 MS.gene62500:CDS 40.0%
AACTCAGTGTTTGTATTGCC+CGG + chr2.1:31271471-31271490 MS.gene62500:intron 40.0%
AATCTAAGTTGTCCTACCAC+CGG - chr2.1:31270334-31270353 None:intergenic 40.0%
ATGGAAGCTATGCATGAAAC+TGG + chr2.1:31269651-31269670 MS.gene62500:CDS 40.0%
ATTACTCGAAAAAAACGGGG+CGG - chr2.1:31271414-31271433 None:intergenic 40.0%
CATACTGATGTCTTATGCTC+AGG - chr2.1:31271918-31271937 None:intergenic 40.0%
CATCAAAATGTTGTCGAAGG+GGG + chr2.1:31271026-31271045 MS.gene62500:intron 40.0%
CCACATCAAAATGTTGTCGA+AGG + chr2.1:31271023-31271042 MS.gene62500:intron 40.0%
CCATAGTTAAGGCCTGTTTA+AGG + chr2.1:31271246-31271265 MS.gene62500:intron 40.0%
CCTTAAACAGGCCTTAACTA+TGG - chr2.1:31271249-31271268 None:intergenic 40.0%
CTTACAAGAATAGCTCCTGT+TGG - chr2.1:31270870-31270889 None:intergenic 40.0%
CTTTGCAAAAATTTGGCGAC+CGG - chr2.1:31270516-31270535 None:intergenic 40.0%
GAAACAGAGAAAGTAGGCAA+AGG - chr2.1:31269564-31269583 None:intergenic 40.0%
GAATTGGATGATCGGGATTA+TGG + chr2.1:31270683-31270702 MS.gene62500:CDS 40.0%
GAGAATGTTTGGTCACTCAT+TGG + chr2.1:31271636-31271655 MS.gene62500:CDS 40.0%
GATCCTAGAAACAATCTCTC+GGG + chr2.1:31271330-31271349 MS.gene62500:intron 40.0%
GATGCTTGGATGATGTTGAT+TGG + chr2.1:31269237-31269256 MS.gene62500:intron 40.0%
GCAATCTGCAATACACATTC+AGG - chr2.1:31271579-31271598 None:intergenic 40.0%
GGGATTACTCGAAAAAAACG+GGG - chr2.1:31271417-31271436 None:intergenic 40.0%
GGGGATTACTCGAAAAAAAC+GGG - chr2.1:31271418-31271437 None:intergenic 40.0%
GTTAACTTCGCCAAGAATTC+AGG - chr2.1:31271675-31271694 None:intergenic 40.0%
GTTCATGGTGGTTCCATATA+AGG - chr2.1:31271701-31271720 None:intergenic 40.0%
TAAAGGAAGAGCCTTTATGG+TGG + chr2.1:31268667-31268686 MS.gene62500:intron 40.0%
TAATGATCTGCTCTCGATTG+AGG - chr2.1:31270185-31270204 None:intergenic 40.0%
TACACCCATTGTGGAGTTAT+GGG - chr2.1:31270986-31271005 None:intergenic 40.0%
TCGATCCAGTAAAGGTGAAT+TGG + chr2.1:31270667-31270686 MS.gene62500:CDS 40.0%
TGCATGCGTTTCTTTCCAAT+CGG - chr2.1:31270310-31270329 None:intergenic 40.0%
TGCCTACTTTCTCTGTTTCA+GGG + chr2.1:31269565-31269584 MS.gene62500:intron 40.0%
TGGTTGAGAAACATGTCTTC+TGG - chr2.1:31271798-31271817 None:intergenic 40.0%
TTACACCCATTGTGGAGTTA+TGG - chr2.1:31270987-31271006 None:intergenic 40.0%
TTCCCTGAAACAGAGAAAGT+AGG - chr2.1:31269570-31269589 None:intergenic 40.0%
TTGCCTACTTTCTCTGTTTC+AGG + chr2.1:31269564-31269583 MS.gene62500:intron 40.0%
! AGACAGTGATGGAGAATGTT+TGG + chr2.1:31271625-31271644 MS.gene62500:CDS 40.0%
! ATCGGATAGTGATATCGGTT+TGG - chr2.1:31270292-31270311 None:intergenic 40.0%
! GCACCCCTATTTGATAACAT+TGG + chr2.1:31269966-31269985 MS.gene62500:intron 40.0%
! TATCCCGAGAGATTGTTTCT+AGG - chr2.1:31271336-31271355 None:intergenic 40.0%
! TCTCTTTTGGATCTCTTGTC+TGG + chr2.1:31270229-31270248 MS.gene62500:CDS 40.0%
! TTCTAGCTGTCAAGTTCTTC+TGG + chr2.1:31270835-31270854 MS.gene62500:CDS 40.0%
!! AAAAAGTGCTTGTATCGTCC+GGG - chr2.1:31271492-31271511 None:intergenic 40.0%
!! CCTTCGACAACATTTTGATG+TGG - chr2.1:31271026-31271045 None:intergenic 40.0%
!! TGTGCTTGATTTGTTCATGG+TGG - chr2.1:31271713-31271732 None:intergenic 40.0%
!! TTGCAGATTTTGATCGAGTC+CGG + chr2.1:31270494-31270513 MS.gene62500:CDS 40.0%
!!! CATGAAACTGGTTTTTTCCC+AGG + chr2.1:31269663-31269682 MS.gene62500:CDS 40.0%
!!! CATTGTTTTTGCATCCGTGA+TGG + chr2.1:31270460-31270479 MS.gene62500:CDS 40.0%
AAACAAGAGAGACTCCTCGA+AGG + chr2.1:31269618-31269637 MS.gene62500:CDS 45.0%
AACAGCACCACCTGAATTCT+TGG + chr2.1:31271662-31271681 MS.gene62500:CDS 45.0%
AACTTCGCCAAGAATTCAGG+TGG - chr2.1:31271672-31271691 None:intergenic 45.0%
ACTTGCAAAGAGTGAGAGGA+CGG + chr2.1:31270105-31270124 MS.gene62500:CDS 45.0%
AGCAACTTGCAAAGAGTGAG+AGG + chr2.1:31270101-31270120 MS.gene62500:CDS 45.0%
AGTTCAAGAGCGGTTAAAGG+CGG - chr2.1:31271190-31271209 None:intergenic 45.0%
CACATGGACAAAGACAGTGA+TGG + chr2.1:31271614-31271633 MS.gene62500:CDS 45.0%
CCTAGTTCAAGAGCGGTTAA+AGG - chr2.1:31271193-31271212 None:intergenic 45.0%
CCTTTAACCGCTCTTGAACT+AGG + chr2.1:31271190-31271209 MS.gene62500:intron 45.0%
CTCAACCAAGCACACAACAT+CGG + chr2.1:31271810-31271829 MS.gene62500:CDS 45.0%
CTGGCAAGACCTTTGCATAA+TGG + chr2.1:31271159-31271178 MS.gene62500:intron 45.0%
GAACTTGACAGCTAGAACAG+CGG - chr2.1:31270832-31270851 None:intergenic 45.0%
GATCAAAATCTGCAAGCCCA+AGG - chr2.1:31270489-31270508 None:intergenic 45.0%
GCGGTTGAACCATTATGCAA+AGG - chr2.1:31271171-31271190 None:intergenic 45.0%
GTGTGGGTATGTCAGAATCA+CGG - chr2.1:31268442-31268461 None:intergenic 45.0%
GTTTCGTCCTAGTTCAAGAG+CGG - chr2.1:31271200-31271219 None:intergenic 45.0%
TAGCTGTCAAGTTCTTCTGG+TGG + chr2.1:31270838-31270857 MS.gene62500:CDS 45.0%
TGGAAAGAAACGCATGCAAC+CGG + chr2.1:31270312-31270331 MS.gene62500:CDS 45.0%
TGTCAAGTTCTTCTGGTGGA+TGG + chr2.1:31270842-31270861 MS.gene62500:CDS 45.0%
TGTTTCAGGGAAACAACGCA+AGG + chr2.1:31269578-31269597 MS.gene62500:intron 45.0%
! ACCTTTACTGGATCGACTTC+TGG - chr2.1:31270663-31270682 None:intergenic 45.0%
! AGCGTGTCAATTGCGGATTA+CGG + chr2.1:31269804-31269823 MS.gene62500:intron 45.0%
! CGGTCGCCAAATTTTTGCAA+AGG + chr2.1:31270514-31270533 MS.gene62500:CDS 45.0%
! CTTCTCTGAGCCCATTTTCT+GGG + chr2.1:31271372-31271391 MS.gene62500:intron 45.0%
! TCTTCTCTGAGCCCATTTTC+TGG + chr2.1:31271371-31271390 MS.gene62500:intron 45.0%
!! AATTGAACCGAGCATCGAAC+TGG + chr2.1:31271140-31271159 MS.gene62500:intron 45.0%
!! ATATCGGTTTGGCTTGCTCA+TGG - chr2.1:31270281-31270300 None:intergenic 45.0%
!! CTTGGTGTTGTTTGCAGCAA+AGG + chr2.1:31270153-31270172 MS.gene62500:CDS 45.0%
!!! GAAACTGGTTTTTTCCCAGG+AGG + chr2.1:31269666-31269685 MS.gene62500:CDS 45.0%
AAAGAAACGCATGCAACCGG+TGG + chr2.1:31270315-31270334 MS.gene62500:CDS 50.0%
GCATGAGGCTCCCAGAAAAT+GGG - chr2.1:31271385-31271404 None:intergenic 50.0%
GCCAGAAGTCGATCCAGTAA+AGG + chr2.1:31270659-31270678 MS.gene62500:CDS 50.0%
TGATGCCTGGTGCAACAGTA+AGG + chr2.1:31271083-31271102 MS.gene62500:intron 50.0%
TGCATGAGGCTCCCAGAAAA+TGG - chr2.1:31271386-31271405 None:intergenic 50.0%
TTCAGGGAAACAACGCAAGG+TGG + chr2.1:31269581-31269600 MS.gene62500:intron 50.0%
TTTCGCCGATGTTGTGTGCT+TGG - chr2.1:31271818-31271837 None:intergenic 50.0%
! TTCTGGTGGATGGATCCAAC+AGG + chr2.1:31270852-31270871 MS.gene62500:CDS 50.0%
!! ACTAGCCTTACTGTTGCACC+AGG - chr2.1:31271091-31271110 None:intergenic 50.0%
AAACGCATGCAACCGGTGGT+AGG + chr2.1:31270319-31270338 MS.gene62500:intron 55.0%
ATCACCTCAGAGAGACCTCC+TGG - chr2.1:31269684-31269703 None:intergenic 55.0%
CAAGCCCAAGGTTGCCATCA+CGG - chr2.1:31270477-31270496 None:intergenic 55.0%
CTTGACAGCTAGAACAGCGG+CGG - chr2.1:31270829-31270848 None:intergenic 55.0%
GCATCCGTGATGGCAACCTT+GGG + chr2.1:31270470-31270489 MS.gene62500:CDS 55.0%
GCTAGTTAGAGGGTGATGCC+TGG + chr2.1:31271070-31271089 MS.gene62500:intron 55.0%
GGTCTTGCCAGTTCGATGCT+CGG - chr2.1:31271150-31271169 None:intergenic 55.0%
TCACCTCAGAGAGACCTCCT+GGG - chr2.1:31269683-31269702 None:intergenic 55.0%
TGCATCCGTGATGGCAACCT+TGG + chr2.1:31270469-31270488 MS.gene62500:CDS 55.0%
TTTCCCAGGAGGTCTCTCTG+AGG + chr2.1:31269677-31269696 MS.gene62500:CDS 55.0%
AACAGCGGCGGCTAATCCGA+CGG - chr2.1:31270817-31270836 None:intergenic 60.0%
ACGGGGCGGCTTAATGCATG+AGG - chr2.1:31271400-31271419 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 31267891 31271938 31267891 ID=MS.gene62500
chr2.1 mRNA 31267891 31271938 31267891 ID=MS.gene62500.t1;Parent=MS.gene62500
chr2.1 exon 31267891 31268050 31267891 ID=MS.gene62500.t1.exon1;Parent=MS.gene62500.t1
chr2.1 CDS 31267891 31268050 31267891 ID=cds.MS.gene62500.t1;Parent=MS.gene62500.t1
chr2.1 exon 31269586 31269698 31269586 ID=MS.gene62500.t1.exon2;Parent=MS.gene62500.t1
chr2.1 CDS 31269586 31269698 31269586 ID=cds.MS.gene62500.t1;Parent=MS.gene62500.t1
chr2.1 exon 31270091 31270336 31270091 ID=MS.gene62500.t1.exon3;Parent=MS.gene62500.t1
chr2.1 CDS 31270091 31270336 31270091 ID=cds.MS.gene62500.t1;Parent=MS.gene62500.t1
chr2.1 exon 31270455 31270535 31270455 ID=MS.gene62500.t1.exon4;Parent=MS.gene62500.t1
chr2.1 CDS 31270455 31270535 31270455 ID=cds.MS.gene62500.t1;Parent=MS.gene62500.t1
chr2.1 exon 31270654 31270884 31270654 ID=MS.gene62500.t1.exon5;Parent=MS.gene62500.t1
chr2.1 CDS 31270654 31270884 31270654 ID=cds.MS.gene62500.t1;Parent=MS.gene62500.t1
chr2.1 exon 31271594 31271938 31271594 ID=MS.gene62500.t1.exon6;Parent=MS.gene62500.t1
chr2.1 CDS 31271594 31271938 31271594 ID=cds.MS.gene62500.t1;Parent=MS.gene62500.t1
Gene Sequence

>MS.gene62500

ATGGCTGATAGTGATAGCATACGAAGTGAACCTCTTCTTGATGAAGTTTCAAACGGTTCTTGGAGACTTAATGTTAAAGAATTTCATCTACCATCACAGAATGTTGTTGATCATGAAAAGAAATCTAAGTTTAGTTTCAATGGTCTTCTTCGTAAGCCTAGTGAGTCATTCTTAAATTTATGTTCTAAAGTTTATGTTTTTTTGGTGTGTTGAATTGTGTGAACCGACCCATTATGTTGTTCTTTGCTTTTTTCAAGATTTTCTTTCATTGATTGATTAAAGTTTCAATTTTGTGTGATGGGTTTGTTCAGTTTTCTGTGATTATGTTGTGCATGTGTTGCTGTTGAATTCTGCAATAAGCCATTGTTAATCCTTTTTCACCTATTTTTTTCTTTTTCTGTTTAAAGTTTTCTACTTTTGTGTAATGCTTATGTTTTGTTTTTCATGTGTATGTTTAATATTAAGGTGGATATGTCATGTCAGAATCACTATGATTTACCATGATTTTTACTACAAAGTCTAGCTTTTGGAAAAATCACGGTGAGTCACCGTGATTCTGACATACCCACACATATTAAACATAAACTATAGTTATGTTTATGTTGTGTATTGAATTCTGCAAATTCATCCTTTTAAATTTTCTTGTATGGTAAAAACTTTTAGATTCTCTGTTTTTTGGTGGTGATTAGTTGAATTGTGCAAACCTTAAAAAGTAAGATCATGATAATTTTGAATTTTTGTGTAGTTTTATTGTAATAATAATGGGGATCTTTAATTAAAGGAAGAGCCTTTATGGTGGATATTCATCAATTTATAGTGCTATTATTATTTGATTTGTTGCAAAAGTAGATGATTGATAAAGATCATGTTTTTTTGTTTTAATTTTCAAGTTGCTGTTACTGTTTATGAATATACTCCATTATTTTATTTTGTTGTTGCTTTAATCCATTTGTACACAATCAAAGCTTAATCAGATATGAATCTTCCTTCACTTAATTCCTTTACACATCATTATTTTGTCATGTACCTAATTTATAATATTTTTTTAAAGGATAAAATAGTTTTTCATGATAAGTACTTAACAAAAGTTTGTGTGCCATTACCTTGTTCACATAATAAAGTTAACATTTAAAATATTTATTTTTTAATAAGTTGTTGATATGAAAATTCTGAAATTTCTGGTTATCAATTTACCTGAGAAGCTGGTAAAAAATTAGCAGTTAAAAATTTGTGTTTAAAATCAAGTTAAGATTGCTAGTTTCAAGCATCATGCATGACTTAAAGTGTATGTTTGGTTTCATGGTAGAGAGTCAGAAGTGATTTCAGACACATAAAACTGATTTAAAGATGCTTGGATGATGTTGATTGGAATTGATTTTGCCACTAGAAAATATTCCAATAGAATTTGTAGCTTTTGACAATTAAGGTGATTTTTATTATCAATTTTTTTTTTCAGGTGAAGGGTTTATTACATTGTTATCAAATTATTTTTCAACTTGCTTTTAGGTGAATGTTTTTTGTATCCAAACATAAACACTTCACATTCAACTAACTTTTAACTAAAATTAATTTTACAAAATCATTTTTTGTCACCGCAAAATCAAACACATACTAGTTTATTAAGTGGTTTTTTTCTTTTTCTGAAGTTTTTGATTATGTTCTGACTTTTGCCTTTGCCTACTTTCTCTGTTTCAGGGAAACAACGCAAGGTGGCCGAGTATTACAAGAAACAAGAGAGACTCCTCGAAGGATTTAATGAAATGGAAGCTATGCATGAAACTGGTTTTTTCCCAGGAGGTCTCTCTGAGGTGATTGTCTATTAATCACTATGATTTACTTACAATTTTCTTATAAACGGAGATGCATTTAACTAGTTTTGATGACTGTAATGTCACTGCAAAACTGAGATTTTTAGCGTGTCAATTGCGGATTACGGAAAATATCGGTTTGTGCATATTCTGCTACGCTACAGTGCTATAGCACTATTATAACCACTATTTGACCACACTTTATACTAAATCGTGTATTGCCGAATAATAACGGTTTGTTTAAATTTACTATGCTATAGTGCTATAGCACCCCTATTTGATAACATTGGTGATTTCCAATTGAACCTAATCGATTCAAACACATGTAGATGAATGATTGAATTTAGTCGATAAGAGAATTCTACTGAATAAAATTGCATTATGTTTGTCAGGATGAAATGAAGCAACTTGCAAAGAGTGAGAGGACGGCAGTACTTGTGTCAAATGCATGTAACTTGGTGTTGTTTGCAGCAAAGGTTTTCGCCTCAATCGAGAGCAGATCATTAGCAGTCATTGCATCAACACTAGACTCTCTTTTGGATCTCTTGTCTGGTTTCATATTGTGGTTTACTTCAAATGCCATGAGCAAGCCAAACCGATATCACTATCCGATTGGAAAGAAACGCATGCAACCGGTGGTAGGACAACTTAGATTTTAAACTGCAACGCGATTGATGAGTCGTTTAAAATTTTAGTAAATGCAGTAGTTTCATAATACACTGAGTGTGAAACACAAATACTTATGTTTCTGTGCAGGGTATCATTGTTTTTGCATCCGTGATGGCAACCTTGGGCTTGCAGATTTTGATCGAGTCCGGTCGCCAAATTTTTGCAAAGGTAATCACAGATCAGCTTTTCTTGTTATTATTTGTTCACTAGTTTAAATTTGAGTTTAATCGTTTTTGTCGTGCGTAGCAGTGTTATACATTTCTCAGTATCATTCTTTTTCTTACAGGCTAAGCCAGAAGTCGATCCAGTAAAGGTGAATTGGATGATCGGGATTATGGTATTTGTAACTGTAGTGAAGTTCATTCTTATGATCTATTGTCGAAGATTCAAAAATGAAATTGTCAGAGCATATGCACAAGATCATTTTTTCGATGTCATTACTAATTCCGTCGGATTAGCCGCCGCTGTTCTAGCTGTCAAGTTCTTCTGGTGGATGGATCCAACAGGAGCTATTCTTGTAAGTTTATAACTTCATCTTATTTTGTTCGCATTTCAGTGTGGTTTTCTGATTGTGTTAACGTGTTCCACAATTAGTATTTTTCCATTAATAAATTTCCCATAACTCCACAATGGGTGTAAACTCCTTTCGTATAAGCCACATCAAAATGTTGTCGAAGGGGGTTTTGTGTCACATGATTGTTTGCTAGTTAGAGGGTGATGCCTGGTGCAACAGTAAGGCTAGTGTCTTGTGACCTAGAAGCAATGCTTTAAAAATTGAACCGAGCATCGAACTGGCAAGACCTTTGCATAATGGTTCAACCGCCTTTAACCGCTCTTGAACTAGGACGAAACATCTATCAAACCGCCAAAATAACTGACCATAGTTAAGGCCTGTTTAAGGTTTAATTAGTTCAACTGTTTTGGTTCGGTTTTTAAAACATTGTTTGAAAGTTACAGATTCAGATCCTAGAAACAATCTCTCGGGATAAACTGTGTATATCTATCTTCTCTGAGCCCATTTTCTGGGAGCCTCATGCATTAAGCCGCCCCGTTTTTTTCGAGTAATCCCCAAAGTTACTTCAGAAGTACACATTGAGAAAACAAACTCAGTGTTTGTATTGCCCGGACGATACAAGCACTTTTTTTATATGATCGTTGGTTACTTCATGCAAACTCCATGATTGATGTACTAATGATGATTATATTACCCTGAATGTGTATTGCAGATTGCACTATATACAATCAACACATGGACAAAGACAGTGATGGAGAATGTTTGGTCACTCATTGGAAGAACAGCACCACCTGAATTCTTGGCGAAGTTAACTTACCTTATATGGAACCACCATGAACAAATCAAGCACATAGATACAGTTAGAGCATACACATTTGGTGCAGATTACTTTGTGGAAGTTGACATTGTTTTACCAGAAGACATGTTTCTCAACCAAGCACACAACATCGGCGAAACACTTCAAGAGAAGCTAGAACAACTTCCAGAAGTTGAAAGAGCTTTTGTTCACATTGATTTTGAGTTCACTCATAGACCTGAGCATAAGACATCAGTATGA
Protein sequence

>MS.gene62500.t1

MADSDSIRSEPLLDEVSNGSWRLNVKEFHLPSQNVVDHEKKSKFSFNGLLRKPRKQRKVAEYYKKQERLLEGFNEMEAMHETGFFPGGLSEDEMKQLAKSERTAVLVSNACNLVLFAAKVFASIESRSLAVIASTLDSLLDLLSGFILWFTSNAMSKPNRYHYPIGKKRMQPVGIIVFASVMATLGLQILIESGRQIFAKAKPEVDPVKVNWMIGIMVFVTVVKFILMIYCRRFKNEIVRAYAQDHFFDVITNSVGLAAAVLAVKFFWWMDPTGAILIALYTINTWTKTVMENVWSLIGRTAPPEFLAKLTYLIWNHHEQIKHIDTVRAYTFGADYFVEVDIVLPEDMFLNQAHNIGETLQEKLEQLPEVERAFVHIDFEFTHRPEHKTSV