Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62565.t1 | XP_004508161.1 | 98.4 | 61 | 1 | 0 | 1 | 61 | 1 | 61 | 3.40E-24 | 120.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62565.t1 | Q9M7X7 | 83.6 | 61 | 10 | 0 | 1 | 61 | 1 | 61 | 8.8e-21 | 100.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62565.t1 | I3SZB6 | 98.4 | 61 | 1 | 0 | 1 | 61 | 1 | 61 | 2.5e-24 | 120.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene043645 | MS.gene62565 | PPI |
| MS.gene035826 | MS.gene62565 | PPI |
| MS.gene62565 | MS.gene73093 | PPI |
| MS.gene064351 | MS.gene62565 | PPI |
| MS.gene62565 | MS.gene035825 | PPI |
| MS.gene99872 | MS.gene62565 | PPI |
| MS.gene043643 | MS.gene62565 | PPI |
| MS.gene035828 | MS.gene62565 | PPI |
| MS.gene62565 | MS.gene043595 | PPI |
| MS.gene043593 | MS.gene62565 | PPI |
| MS.gene064353 | MS.gene62565 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62565.t1 | MTR_4g121877 | 98.361 | 61 | 1 | 0 | 1 | 61 | 1 | 61 | 7.37e-39 | 122 |
| MS.gene62565.t1 | MTR_4g121870 | 93.443 | 61 | 4 | 0 | 1 | 61 | 137 | 197 | 1.26e-36 | 121 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62565.t1 | AT3G06700 | 83.607 | 61 | 10 | 0 | 1 | 61 | 1 | 61 | 2.28e-31 | 103 |
| MS.gene62565.t1 | AT3G06700 | 83.607 | 61 | 10 | 0 | 1 | 61 | 1 | 61 | 2.28e-31 | 103 |
| MS.gene62565.t1 | AT3G06700 | 83.607 | 61 | 10 | 0 | 1 | 61 | 1 | 61 | 2.28e-31 | 103 |
| MS.gene62565.t1 | AT3G06680 | 83.607 | 61 | 10 | 0 | 1 | 61 | 23 | 83 | 2.54e-31 | 104 |
| MS.gene62565.t1 | AT3G06680 | 83.607 | 61 | 10 | 0 | 1 | 61 | 1 | 61 | 4.88e-31 | 102 |
| MS.gene62565.t1 | AT3G06680 | 83.607 | 61 | 10 | 0 | 1 | 61 | 1 | 61 | 4.88e-31 | 102 |
Find 14 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGTGTGGCGATGTCTCTT+TGG | 0.360027 | 7.3:-69002316 | None:intergenic |
| CTGATAAATACAAACTCCTT+TGG | 0.392523 | 7.3:-69002341 | None:intergenic |
| CTCTGGTTCCTCAAGAACTT+TGG | 0.404366 | 7.3:-69002447 | None:intergenic |
| TCTTGTGAGCCTTGTAAGAC+TGG | 0.428046 | 7.3:-69002279 | None:intergenic |
| TGTGCTTCCTTGCATACCTC+TGG | 0.438138 | 7.3:-69002464 | None:intergenic |
| CGCTCACAACCAGTCTTACA+AGG | 0.489194 | 7.3:+69002270 | MS.gene62565:CDS |
| ATAAATACAAACTCCTTTGG+TGG | 0.508253 | 7.3:-69002338 | None:intergenic |
| TCTTACAAGGCTCACAAGAA+TGG | 0.521704 | 7.3:+69002283 | MS.gene62565:CDS |
| AACAATTTACTCTTCTTCAG+TGG | 0.567212 | 7.3:-69002508 | None:intergenic |
| AGATGGATCCAAAGTTCTTG+AGG | 0.579402 | 7.3:+69002439 | MS.gene62565:intron |
| CATCGCCACACTTCCACCAA+AGG | 0.596196 | 7.3:+69002325 | MS.gene62565:CDS |
| AAACTCCTTTGGTGGAAGTG+TGG | 0.614881 | 7.3:-69002330 | None:intergenic |
| TGAGGAACCAGAGGTATGCA+AGG | 0.624057 | 7.3:+69002457 | MS.gene62565:CDS |
| CAAAGTTCTTGAGGAACCAG+AGG | 0.651910 | 7.3:+69002448 | MS.gene62565:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATTTTTATAATTGTAGA+TGG | + | chr7.3:69002422-69002441 | MS.gene62565:intron | 10.0% |
| ATAAATACAAACTCCTTTGG+TGG | - | chr7.3:69002341-69002360 | None:intergenic | 30.0% | |
| CTGATAAATACAAACTCCTT+TGG | - | chr7.3:69002344-69002363 | None:intergenic | 30.0% | |
| ! | AGGAAGCACAACAAGAAAAA+CGG | + | chr7.3:69002477-69002496 | MS.gene62565:CDS | 35.0% |
| AGATGGATCCAAAGTTCTTG+AGG | + | chr7.3:69002439-69002458 | MS.gene62565:intron | 40.0% | |
| TCTTACAAGGCTCACAAGAA+TGG | + | chr7.3:69002283-69002302 | MS.gene62565:CDS | 40.0% | |
| AAACTCCTTTGGTGGAAGTG+TGG | - | chr7.3:69002333-69002352 | None:intergenic | 45.0% | |
| CAAAGTTCTTGAGGAACCAG+AGG | + | chr7.3:69002448-69002467 | MS.gene62565:CDS | 45.0% | |
| ! | CTCTGGTTCCTCAAGAACTT+TGG | - | chr7.3:69002450-69002469 | None:intergenic | 45.0% |
| ! | TCTTGTGAGCCTTGTAAGAC+TGG | - | chr7.3:69002282-69002301 | None:intergenic | 45.0% |
| CGCTCACAACCAGTCTTACA+AGG | + | chr7.3:69002270-69002289 | MS.gene62565:CDS | 50.0% | |
| GAAGTGTGGCGATGTCTCTT+TGG | - | chr7.3:69002319-69002338 | None:intergenic | 50.0% | |
| TGTGCTTCCTTGCATACCTC+TGG | - | chr7.3:69002467-69002486 | None:intergenic | 50.0% | |
| ! | TGAGGAACCAGAGGTATGCA+AGG | + | chr7.3:69002457-69002476 | MS.gene62565:CDS | 50.0% |
| CATCGCCACACTTCCACCAA+AGG | + | chr7.3:69002325-69002344 | MS.gene62565:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 69002247 | 69002524 | 69002247 | ID=MS.gene62565 |
| chr7.3 | mRNA | 69002247 | 69002524 | 69002247 | ID=MS.gene62565.t1;Parent=MS.gene62565 |
| chr7.3 | exon | 69002247 | 69002348 | 69002247 | ID=MS.gene62565.t1.exon1;Parent=MS.gene62565.t1 |
| chr7.3 | CDS | 69002247 | 69002348 | 69002247 | ID=cds.MS.gene62565.t1;Parent=MS.gene62565.t1 |
| chr7.3 | exon | 69002441 | 69002524 | 69002441 | ID=MS.gene62565.t1.exon2;Parent=MS.gene62565.t1 |
| chr7.3 | CDS | 69002441 | 69002524 | 69002441 | ID=cds.MS.gene62565.t1;Parent=MS.gene62565.t1 |
| Gene Sequence |
| Protein sequence |
