Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65402.t1 | RHN58352.1 | 80.4 | 607 | 36 | 3 | 1 | 531 | 113 | 712 | 4.70E-271 | 943.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65402.t1 | Q9SWH5 | 40.3 | 472 | 192 | 5 | 139 | 526 | 93 | 558 | 2.8e-86 | 320.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65402.t1 | G7JEG8 | 80.4 | 607 | 36 | 3 | 1 | 531 | 1 | 600 | 3.4e-271 | 943.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050750 | MS.gene65402 | 0.802507 | 5.43E-49 | -1.69E-46 |
| MS.gene050820 | MS.gene65402 | 0.811848 | 5.70E-51 | -1.69E-46 |
| MS.gene050953 | MS.gene65402 | 0.801301 | 9.61E-49 | -1.69E-46 |
| MS.gene052190 | MS.gene65402 | 0.811163 | 8.03E-51 | -1.69E-46 |
| MS.gene052333 | MS.gene65402 | 0.836715 | 7.96E-57 | -1.69E-46 |
| MS.gene053166 | MS.gene65402 | 0.807552 | 4.78E-50 | -1.69E-46 |
| MS.gene053201 | MS.gene65402 | 0.849784 | 2.59E-60 | -1.69E-46 |
| MS.gene05415 | MS.gene65402 | 0.802224 | 6.21E-49 | -1.69E-46 |
| MS.gene054467 | MS.gene65402 | 0.81717 | 3.79E-52 | -1.69E-46 |
| MS.gene054468 | MS.gene65402 | 0.804063 | 2.59E-49 | -1.69E-46 |
| MS.gene054559 | MS.gene65402 | 0.817382 | 3.39E-52 | -1.69E-46 |
| MS.gene057194 | MS.gene65402 | 0.80841 | 3.14E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene65402 | 0.81098 | 8.80E-51 | -1.69E-46 |
| MS.gene059272 | MS.gene65402 | 0.805321 | 1.41E-49 | -1.69E-46 |
| MS.gene060011 | MS.gene65402 | 0.800485 | 1.41E-48 | -1.69E-46 |
| MS.gene060167 | MS.gene65402 | 0.80061 | 1.33E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65402.t1 | MTR_4g007220 | 93.842 | 341 | 16 | 2 | 191 | 531 | 265 | 600 | 0.0 | 671 |
| MS.gene65402.t1 | MTR_4g007220 | 87.179 | 195 | 22 | 1 | 1 | 195 | 1 | 192 | 2.54e-111 | 343 |
| MS.gene65402.t1 | MTR_2g020730 | 65.385 | 338 | 110 | 4 | 191 | 527 | 244 | 575 | 6.09e-154 | 452 |
| MS.gene65402.t1 | MTR_2g020730 | 42.991 | 214 | 86 | 7 | 14 | 222 | 4 | 186 | 7.39e-31 | 126 |
| MS.gene65402.t1 | MTR_1g090430 | 58.769 | 325 | 131 | 1 | 204 | 528 | 165 | 486 | 3.10e-134 | 399 |
| MS.gene65402.t1 | MTR_1g090430 | 63.889 | 72 | 26 | 0 | 124 | 195 | 5 | 76 | 3.77e-21 | 97.1 |
| MS.gene65402.t1 | MTR_8g099865 | 53.030 | 330 | 150 | 3 | 199 | 527 | 257 | 582 | 7.06e-118 | 360 |
| MS.gene65402.t1 | MTR_8g099865 | 36.702 | 188 | 104 | 5 | 16 | 200 | 3 | 178 | 1.67e-24 | 107 |
| MS.gene65402.t1 | MTR_4g108780 | 45.397 | 315 | 165 | 3 | 215 | 528 | 265 | 573 | 1.21e-86 | 278 |
| MS.gene65402.t1 | MTR_4g108780 | 59.259 | 54 | 22 | 0 | 141 | 194 | 108 | 161 | 1.26e-12 | 70.9 |
| MS.gene65402.t1 | MTR_3g037560 | 44.164 | 317 | 171 | 3 | 215 | 531 | 227 | 537 | 1.55e-86 | 277 |
| MS.gene65402.t1 | MTR_4g007380 | 63.063 | 111 | 25 | 2 | 377 | 487 | 8 | 102 | 4.50e-36 | 131 |
| MS.gene65402.t1 | MTR_7g005890 | 50.336 | 149 | 42 | 4 | 274 | 422 | 1 | 117 | 2.62e-35 | 128 |
| MS.gene65402.t1 | MTR_4g007360 | 37.778 | 225 | 70 | 6 | 109 | 274 | 100 | 313 | 1.44e-32 | 127 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65402.t1 | AT2G03220 | 44.615 | 325 | 174 | 2 | 202 | 526 | 240 | 558 | 3.85e-88 | 282 |
| MS.gene65402.t1 | AT2G03220 | 70.000 | 60 | 18 | 0 | 139 | 198 | 93 | 152 | 2.14e-18 | 89.0 |
| MS.gene65402.t1 | AT2G15350 | 43.465 | 329 | 178 | 3 | 201 | 528 | 192 | 513 | 9.73e-88 | 280 |
| MS.gene65402.t1 | AT2G15350 | 53.571 | 56 | 25 | 1 | 141 | 195 | 42 | 97 | 3.05e-12 | 69.3 |
| MS.gene65402.t1 | AT2G15370 | 43.465 | 329 | 180 | 3 | 201 | 528 | 209 | 532 | 1.10e-87 | 280 |
| MS.gene65402.t1 | AT2G15370 | 45.238 | 84 | 35 | 2 | 141 | 223 | 61 | 134 | 5.19e-14 | 75.1 |
| MS.gene65402.t1 | AT2G15390 | 42.249 | 329 | 183 | 4 | 201 | 528 | 212 | 534 | 4.91e-84 | 271 |
| MS.gene65402.t1 | AT2G15390 | 45.455 | 77 | 38 | 2 | 120 | 195 | 45 | 118 | 2.23e-13 | 73.2 |
| MS.gene65402.t1 | AT2G15390 | 42.249 | 329 | 183 | 4 | 201 | 528 | 180 | 502 | 4.97e-84 | 270 |
| MS.gene65402.t1 | AT2G15390 | 54.386 | 57 | 25 | 1 | 140 | 195 | 30 | 86 | 4.62e-13 | 72.0 |
| MS.gene65402.t1 | AT1G14100 | 42.169 | 332 | 182 | 6 | 201 | 530 | 193 | 516 | 3.13e-82 | 265 |
| MS.gene65402.t1 | AT1G14100 | 62.500 | 56 | 21 | 0 | 140 | 195 | 44 | 99 | 9.20e-16 | 80.5 |
| MS.gene65402.t1 | AT2G03210 | 44.586 | 314 | 173 | 1 | 215 | 528 | 226 | 538 | 3.47e-82 | 266 |
| MS.gene65402.t1 | AT2G03210 | 51.250 | 80 | 29 | 2 | 123 | 195 | 45 | 121 | 3.12e-16 | 82.0 |
| MS.gene65402.t1 | AT2G03210 | 44.586 | 314 | 173 | 1 | 215 | 528 | 260 | 572 | 5.69e-82 | 266 |
| MS.gene65402.t1 | AT2G03210 | 51.250 | 80 | 29 | 2 | 123 | 195 | 79 | 155 | 3.26e-16 | 82.0 |
| MS.gene65402.t1 | AT2G03210 | 44.586 | 314 | 173 | 1 | 215 | 528 | 257 | 569 | 6.53e-82 | 266 |
| MS.gene65402.t1 | AT2G03210 | 53.247 | 77 | 29 | 2 | 123 | 195 | 79 | 152 | 1.45e-16 | 83.2 |
| MS.gene65402.t1 | AT1G14080 | 41.509 | 318 | 181 | 2 | 201 | 518 | 213 | 525 | 2.36e-81 | 264 |
| MS.gene65402.t1 | AT1G14080 | 59.649 | 57 | 22 | 1 | 140 | 195 | 63 | 119 | 5.12e-15 | 78.2 |
| MS.gene65402.t1 | AT1G14110 | 38.650 | 326 | 153 | 3 | 201 | 526 | 190 | 468 | 2.41e-72 | 238 |
| MS.gene65402.t1 | AT1G14110 | 54.545 | 55 | 24 | 1 | 141 | 195 | 46 | 99 | 1.98e-11 | 66.6 |
| MS.gene65402.t1 | AT1G74420 | 40.698 | 258 | 150 | 2 | 242 | 498 | 264 | 519 | 7.93e-57 | 198 |
| MS.gene65402.t1 | AT1G74420 | 40.698 | 258 | 150 | 2 | 242 | 498 | 268 | 523 | 8.16e-57 | 198 |
| MS.gene65402.t1 | AT1G74420 | 40.698 | 258 | 150 | 2 | 242 | 498 | 302 | 557 | 1.69e-56 | 198 |
| MS.gene65402.t1 | AT1G74420 | 40.698 | 258 | 150 | 2 | 242 | 498 | 306 | 561 | 1.82e-56 | 198 |
Find 75 sgRNAs with CRISPR-Local
Find 138 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTACTTCTTTGTCTACTTT+TGG | 0.201160 | 4.3:-88172356 | MS.gene65402:CDS |
| TTTAAATTATCACCGTATTC+TGG | 0.239005 | 4.3:+88172530 | None:intergenic |
| ACGAATAAATCAACTTCTTC+AGG | 0.252310 | 4.3:-88172497 | MS.gene65402:CDS |
| AGCTAACAATAATTCAAAAT+TGG | 0.265569 | 4.3:-88173041 | MS.gene65402:CDS |
| CTTGATGGACTCTTAGCTTC+TGG | 0.291465 | 4.3:-88173502 | MS.gene65402:CDS |
| TCAGATGGTGCCAACATAAA+AGG | 0.304553 | 4.3:-88174294 | MS.gene65402:CDS |
| GCTAAAGCAGATGAGAAAAT+AGG | 0.324523 | 4.3:-88172734 | MS.gene65402:CDS |
| TCTTTCATACCAAGTACTTT+TGG | 0.362190 | 4.3:+88172620 | None:intergenic |
| TCCTATCCTTAGTGACTTGA+AGG | 0.372010 | 4.3:+88174526 | None:intergenic |
| CACAAGCTTCTCTCTCTTTG+AGG | 0.384231 | 4.3:-88174412 | MS.gene65402:CDS |
| GGATATGTTGCTCAAGGCTT+GGG | 0.386546 | 4.3:-88172335 | MS.gene65402:CDS |
| CATTTACATGAGGAAATGTA+AGG | 0.397294 | 4.3:-88172162 | MS.gene65402:CDS |
| TACAAACCTGTTGTTGTAGC+TGG | 0.406944 | 4.3:+88173723 | None:intergenic |
| CATAGGTGGTGTGGACCTAA+TGG | 0.418999 | 4.3:-88173367 | MS.gene65402:intron |
| GGAAAAGGTAGCGTCCTAAA+TGG | 0.422451 | 4.3:+88172810 | None:intergenic |
| GATTGAACACTCTTATTTGA+AGG | 0.425603 | 4.3:+88172711 | None:intergenic |
| CTCAAGGCTTGGGAAATTTG+AGG | 0.429093 | 4.3:-88172325 | MS.gene65402:CDS |
| TTCGATATTAAATATGAAGA+AGG | 0.430936 | 4.3:+88173412 | None:intergenic |
| AAAGAATGTTGTAAATCTCT+TGG | 0.434299 | 4.3:+88174491 | None:intergenic |
| GAAAGACCAGCTACAACAAC+AGG | 0.435452 | 4.3:-88173729 | MS.gene65402:intron |
| TGGATATGTTGCTCAAGGCT+TGG | 0.436642 | 4.3:-88172336 | MS.gene65402:CDS |
| CTCTTTGAGGACTTTACAAA+AGG | 0.445621 | 4.3:-88174399 | MS.gene65402:CDS |
| AAAGGCAAGGTACTACTTGT+TGG | 0.448202 | 4.3:-88174381 | MS.gene65402:CDS |
| CGAATAAATCAACTTCTTCA+GGG | 0.450484 | 4.3:-88172496 | MS.gene65402:CDS |
| GCTTCTTGTGGAGTTAAAGT+AGG | 0.453450 | 4.3:+88172689 | None:intergenic |
| TGGTAGTAACTTATTGTGAA+TGG | 0.456552 | 4.3:+88172640 | None:intergenic |
| TTTATGAACTCTTCCTCCAT+TGG | 0.459680 | 4.3:+88174320 | None:intergenic |
| ATGTAGAGTTGGATCATTTG+TGG | 0.462453 | 4.3:+88173555 | None:intergenic |
| GCGTCTTTATATCCAGAATA+CGG | 0.468536 | 4.3:-88172542 | MS.gene65402:CDS |
| TGTTGTAGCTGGTCTTTCTT+CGG | 0.470644 | 4.3:+88173734 | None:intergenic |
| AAATATGAAGAAGGTTTATG+AGG | 0.471182 | 4.3:+88173421 | None:intergenic |
| GCTTGGGAAATTTGAGGCCA+TGG | 0.471407 | 4.3:-88172319 | MS.gene65402:CDS |
| TAAGCATAATATGAAAGCTT+GGG | 0.479892 | 4.3:-88172417 | MS.gene65402:CDS |
| CTTCATTCGAAGGGTGGAAA+AGG | 0.480370 | 4.3:+88172795 | None:intergenic |
| TTTCCACTACAGTAATGCTT+AGG | 0.503251 | 4.3:+88172212 | None:intergenic |
| ACTAAGTTTATCTGATGTGT+TGG | 0.503513 | 4.3:-88172381 | MS.gene65402:CDS |
| CCACTACAGTAATGCTTAGG+AGG | 0.503948 | 4.3:+88172215 | None:intergenic |
| AAGTTAACAATGATTACAAC+AGG | 0.504914 | 4.3:+88174447 | None:intergenic |
| TGGATTGGAGTATGTAGAGT+TGG | 0.505395 | 4.3:+88173544 | None:intergenic |
| ATGATGCCTCATGCGTAAGC+AGG | 0.509599 | 4.3:-88173474 | MS.gene65402:CDS |
| CAAGTCACTAAGGATAGGAT+AGG | 0.517734 | 4.3:-88174522 | MS.gene65402:CDS |
| AGATTCATAATTGCTTCTTG+TGG | 0.518248 | 4.3:+88172677 | None:intergenic |
| ATTACAAATGTCACAACCAA+TGG | 0.518815 | 4.3:-88174336 | MS.gene65402:CDS |
| ATCATTTGTGGAACTTGTCA+TGG | 0.519995 | 4.3:+88173567 | None:intergenic |
| ATAAGCATAATATGAAAGCT+TGG | 0.522629 | 4.3:-88172418 | MS.gene65402:CDS |
| TCTTTCTTCGGTTGTTGTAG+TGG | 0.524722 | 4.3:+88173746 | None:intergenic |
| AAATTTCTATCAAGCACACT+TGG | 0.531776 | 4.3:-88172756 | MS.gene65402:CDS |
| AGTCTCCACGCTTCATTCGA+AGG | 0.532757 | 4.3:+88172785 | None:intergenic |
| GAGAACTCTCTTTCACAAGC+TGG | 0.535540 | 4.3:+88172260 | None:intergenic |
| ATTATCTTGTATAGAAGCCA+TGG | 0.536564 | 4.3:+88172302 | None:intergenic |
| GGAAATCCTTCAAGTCACTA+AGG | 0.543070 | 4.3:-88174532 | MS.gene65402:CDS |
| ATTTCTCGGGTAAATTTGGT+CGG | 0.545196 | 4.3:+88172984 | None:intergenic |
| AATGTTTGTTGAAAAGGAGA+CGG | 0.549584 | 4.3:-88172840 | MS.gene65402:CDS |
| GTCTCCACGCTTCATTCGAA+GGG | 0.553860 | 4.3:+88172786 | None:intergenic |
| CCTCCTAAGCATTACTGTAG+TGG | 0.554289 | 4.3:-88172215 | MS.gene65402:CDS |
| CTTCTTCTTTCATTTACATG+AGG | 0.557175 | 4.3:-88172172 | MS.gene65402:CDS |
| TGAGGACTTTACAAAAGGCA+AGG | 0.572789 | 4.3:-88174394 | MS.gene65402:CDS |
| ATATCAAAGTTTGATAGAGA+AGG | 0.577197 | 4.3:-88173068 | MS.gene65402:CDS |
| AAGTCACTAAGGATAGGATA+GGG | 0.584518 | 4.3:-88174521 | MS.gene65402:CDS |
| TTGTAACCTGCTTACGCATG+AGG | 0.586351 | 4.3:+88173468 | None:intergenic |
| CTTTAATAATACTATGTCAA+AGG | 0.586367 | 4.3:-88174268 | MS.gene65402:intron |
| AGAGTTCATAAAATATCAGA+TGG | 0.593218 | 4.3:-88174309 | MS.gene65402:CDS |
| TCCACGCTTCATTCGAAGGG+TGG | 0.594457 | 4.3:+88172789 | None:intergenic |
| AAAGGTGTCATGAAAAGAGA+AGG | 0.597269 | 4.3:+88172884 | None:intergenic |
| ATAGAAGTTTATCAAGCAAG+TGG | 0.597808 | 4.3:-88172467 | MS.gene65402:CDS |
| TCTGATTGCAAAATTAACAA+AGG | 0.600847 | 4.3:+88172920 | None:intergenic |
| ATCACTAACCAGCTTTACAC+CGG | 0.600952 | 4.3:+88172130 | None:intergenic |
| ACAAATGTCACAACCAATGG+AGG | 0.621737 | 4.3:-88174333 | MS.gene65402:CDS |
| AAGTTACTACCAAAAGTACT+TGG | 0.622114 | 4.3:-88172629 | MS.gene65402:CDS |
| TCCTTCAAGTCACTAAGGAT+AGG | 0.627501 | 4.3:-88174527 | MS.gene65402:CDS |
| TTAGGAGGAGCATGATAACA+TGG | 0.627761 | 4.3:+88172230 | None:intergenic |
| TCCACCCTTCGAATGAAGCG+TGG | 0.629725 | 4.3:-88172790 | MS.gene65402:CDS |
| AGATTGAGAAATAATGATGA+TGG | 0.636757 | 4.3:+88173013 | None:intergenic |
| TTGTGAAAGAGAGTTCTCAA+TGG | 0.648650 | 4.3:-88172255 | MS.gene65402:CDS |
| GAATAAATCAACTTCTTCAG+GGG | 0.654370 | 4.3:-88172495 | MS.gene65402:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTGTCAAAAATAATAAATT+TGG | + | chr4.3:88172671-88172690 | None:intergenic | 10.0% |
| !!! | ATGATTAGTATAATAATTTT+TGG | - | chr4.3:88173018-88173037 | MS.gene65402:CDS | 10.0% |
| !!! | CAATTTGATAATTTTTAAAT+TGG | + | chr4.3:88172733-88172752 | None:intergenic | 10.0% |
| !!! | TGATTAGTATAATAATTTTT+GGG | - | chr4.3:88173019-88173038 | MS.gene65402:CDS | 10.0% |
| !! | AACAATACATAATATATGTA+AGG | - | chr4.3:88172463-88172482 | MS.gene65402:CDS | 15.0% |
| !!! | AATAAGACTTTTTTTTTTTC+TGG | + | chr4.3:88172503-88172522 | None:intergenic | 15.0% |
| !! | AGCTAACAATAATTCAAAAT+TGG | - | chr4.3:88173611-88173630 | MS.gene65402:intron | 20.0% |
| !! | CTTTAATAATACTATGTCAA+AGG | - | chr4.3:88172384-88172403 | MS.gene65402:CDS | 20.0% |
| !! | TTCGATATTAAATATGAAGA+AGG | + | chr4.3:88173243-88173262 | None:intergenic | 20.0% |
| !!! | ACAACTTTTAATTACTCTAT+GGG | + | chr4.3:88172764-88172783 | None:intergenic | 20.0% |
| !!! | CTAAATTCTAAGAACATTAA+GGG | - | chr4.3:88173339-88173358 | MS.gene65402:intron | 20.0% |
| !!! | TACAACTTTTAATTACTCTA+TGG | + | chr4.3:88172765-88172784 | None:intergenic | 20.0% |
| !!! | TCTAAATTCTAAGAACATTA+AGG | - | chr4.3:88173338-88173357 | MS.gene65402:intron | 20.0% |
| !!! | TGAAAAAATGTTTGTTGAAA+AGG | - | chr4.3:88173806-88173825 | MS.gene65402:intron | 20.0% |
| ! | AAAAATTTCTCGGGTAAATT+TGG | + | chr4.3:88173675-88173694 | None:intergenic | 25.0% |
| ! | AAAGAATGTTGTAAATCTCT+TGG | + | chr4.3:88172164-88172183 | None:intergenic | 25.0% |
| ! | AAATATGAAGAAGGTTTATG+AGG | + | chr4.3:88173234-88173253 | None:intergenic | 25.0% |
| ! | AAGTTAACAATGATTACAAC+AGG | + | chr4.3:88172208-88172227 | None:intergenic | 25.0% |
| ! | ACTATGAAAAAATTCATAGG+TGG | - | chr4.3:88173271-88173290 | MS.gene65402:intron | 25.0% |
| ! | AGAGTTCATAAAATATCAGA+TGG | - | chr4.3:88172343-88172362 | MS.gene65402:CDS | 25.0% |
| ! | AGATTGAGAAATAATGATGA+TGG | + | chr4.3:88173642-88173661 | None:intergenic | 25.0% |
| ! | ATAAGCATAATATGAAAGCT+TGG | - | chr4.3:88174234-88174253 | MS.gene65402:intron | 25.0% |
| ! | ATATCAAAGTTTGATAGAGA+AGG | - | chr4.3:88173584-88173603 | MS.gene65402:CDS | 25.0% |
| ! | ATCACAGTGAAAAAATTTCT+CGG | + | chr4.3:88173685-88173704 | None:intergenic | 25.0% |
| ! | GCAACTATGAAAAAATTCAT+AGG | - | chr4.3:88173268-88173287 | MS.gene65402:intron | 25.0% |
| ! | TAAGCATAATATGAAAGCTT+GGG | - | chr4.3:88174235-88174254 | MS.gene65402:intron | 25.0% |
| ! | TAATGTACTTGTCAAATTCA+AGG | - | chr4.3:88172862-88172881 | MS.gene65402:CDS | 25.0% |
| ! | TCTGATTGCAAAATTAACAA+AGG | + | chr4.3:88173735-88173754 | None:intergenic | 25.0% |
| ! | TTTAAATTATCACCGTATTC+TGG | + | chr4.3:88174125-88174144 | None:intergenic | 25.0% |
| !! | AACCAAGTTGAATTCAAAAA+CGG | + | chr4.3:88173519-88173538 | None:intergenic | 25.0% |
| !! | ACATGATTTTCATTCCAAAA+AGG | + | chr4.3:88173558-88173577 | None:intergenic | 25.0% |
| !! | ATTAAAGTCTCCTTTTATGT+TGG | + | chr4.3:88172371-88172390 | None:intergenic | 25.0% |
| !! | GAAGAAGCAAAATCTTTTAT+TGG | + | chr4.3:88174467-88174486 | None:intergenic | 25.0% |
| !! | GCAATTTTTCTTTGTCATTA+TGG | + | chr4.3:88173131-88173150 | None:intergenic | 25.0% |
| !! | TTTTCTTTGTCATTATGGAT+TGG | + | chr4.3:88173126-88173145 | None:intergenic | 25.0% |
| !!! | ATGTATTGTTTTATCAACTG+AGG | + | chr4.3:88172453-88172472 | None:intergenic | 25.0% |
| !!! | CAACTTTTAATTACTCTATG+GGG | + | chr4.3:88172763-88172782 | None:intergenic | 25.0% |
| AAATTTCTATCAAGCACACT+TGG | - | chr4.3:88173896-88173915 | MS.gene65402:intron | 30.0% | |
| ACGAATAAATCAACTTCTTC+AGG | - | chr4.3:88174155-88174174 | MS.gene65402:intron | 30.0% | |
| ACTAAGTTTATCTGATGTGT+TGG | - | chr4.3:88174271-88174290 | MS.gene65402:CDS | 30.0% | |
| AGAGATATTAATGCTACAAC+AGG | + | chr4.3:88172587-88172606 | None:intergenic | 30.0% | |
| AGATTCATAATTGCTTCTTG+TGG | + | chr4.3:88173978-88173997 | None:intergenic | 30.0% | |
| ATAGAAGTTTATCAAGCAAG+TGG | - | chr4.3:88174185-88174204 | MS.gene65402:intron | 30.0% | |
| ATTACAAATGTCACAACCAA+TGG | - | chr4.3:88172316-88172335 | MS.gene65402:CDS | 30.0% | |
| ATTATCTTGTATAGAAGCCA+TGG | + | chr4.3:88174353-88174372 | None:intergenic | 30.0% | |
| CAAAGTGCAAATACCTTATT+TGG | - | chr4.3:88173370-88173389 | MS.gene65402:CDS | 30.0% | |
| CATAATATATGTAAGGTCCA+AGG | - | chr4.3:88172470-88172489 | MS.gene65402:CDS | 30.0% | |
| CATTTACATGAGGAAATGTA+AGG | - | chr4.3:88174490-88174509 | MS.gene65402:CDS | 30.0% | |
| CGAATAAATCAACTTCTTCA+GGG | - | chr4.3:88174156-88174175 | MS.gene65402:intron | 30.0% | |
| CGAGCTGAATATAAATATCA+TGG | + | chr4.3:88172963-88172982 | None:intergenic | 30.0% | |
| CTTCTTCTTTCATTTACATG+AGG | - | chr4.3:88174480-88174499 | MS.gene65402:CDS | 30.0% | |
| GAATAAATCAACTTCTTCAG+GGG | - | chr4.3:88174157-88174176 | MS.gene65402:intron | 30.0% | |
| GACAAAGAAAAATTGCTTGA+TGG | - | chr4.3:88173135-88173154 | MS.gene65402:intron | 30.0% | |
| TAATGGTTTAGGAAACCAAA+TGG | - | chr4.3:88173401-88173420 | MS.gene65402:CDS | 30.0% | |
| TATTAATGCTACAACAGGTT+TGG | + | chr4.3:88172582-88172601 | None:intergenic | 30.0% | |
| TCACAGTGAAAAAATTTCTC+GGG | + | chr4.3:88173684-88173703 | None:intergenic | 30.0% | |
| TGGTAGTAACTTATTGTGAA+TGG | + | chr4.3:88174015-88174034 | None:intergenic | 30.0% | |
| ! | AATGTTTGTTGAAAAGGAGA+CGG | - | chr4.3:88173812-88173831 | MS.gene65402:intron | 30.0% |
| ! | ACTACTTCTTTGTCTACTTT+TGG | - | chr4.3:88174296-88174315 | MS.gene65402:CDS | 30.0% |
| ! | GAATATTTCTCATGCTTTTG+TGG | + | chr4.3:88173320-88173339 | None:intergenic | 30.0% |
| ! | GATTGAACACTCTTATTTGA+AGG | + | chr4.3:88173944-88173963 | None:intergenic | 30.0% |
| ! | TCTTTCATACCAAGTACTTT+TGG | + | chr4.3:88174035-88174054 | None:intergenic | 30.0% |
| ! | TGAGGAAATGTAAGGATTTT+TGG | - | chr4.3:88174498-88174517 | MS.gene65402:CDS | 30.0% |
| !! | AAGTTACTACCAAAAGTACT+TGG | - | chr4.3:88174023-88174042 | MS.gene65402:intron | 30.0% |
| !! | AATCAGTTTTGTGCTATGAT+TGG | + | chr4.3:88172555-88172574 | None:intergenic | 30.0% |
| !!! | AAATGTAAGGATTTTTGGTC+CGG | - | chr4.3:88174503-88174522 | MS.gene65402:CDS | 30.0% |
| AAGTCACTAAGGATAGGATA+GGG | - | chr4.3:88172131-88172150 | MS.gene65402:CDS | 35.0% | |
| AGATTCAGGAAAGACAAACA+AGG | - | chr4.3:88173480-88173499 | MS.gene65402:CDS | 35.0% | |
| ATCATTTGTGGAACTTGTCA+TGG | + | chr4.3:88173088-88173107 | None:intergenic | 35.0% | |
| ATGTAGAGTTGGATCATTTG+TGG | + | chr4.3:88173100-88173119 | None:intergenic | 35.0% | |
| ATTTCTCGGGTAAATTTGGT+CGG | + | chr4.3:88173671-88173690 | None:intergenic | 35.0% | |
| CAAAAACAAAGCGATTGTGA+AGG | - | chr4.3:88174076-88174095 | MS.gene65402:intron | 35.0% | |
| GAAAAAATTCATAGGTGGTG+TGG | - | chr4.3:88173276-88173295 | MS.gene65402:intron | 35.0% | |
| GCGTCTTTATATCCAGAATA+CGG | - | chr4.3:88174110-88174129 | MS.gene65402:intron | 35.0% | |
| GCTAAAGCAGATGAGAAAAT+AGG | - | chr4.3:88173918-88173937 | MS.gene65402:intron | 35.0% | |
| TCAAGTTCCTTGCTGAAAAA+AGG | + | chr4.3:88173789-88173808 | None:intergenic | 35.0% | |
| TGAATCTTACAAGCATCACA+CGG | + | chr4.3:88173467-88173486 | None:intergenic | 35.0% | |
| TTGTGAAAGAGAGTTCTCAA+TGG | - | chr4.3:88174397-88174416 | MS.gene65402:CDS | 35.0% | |
| TTGTGGTAATCCTTACCATT+AGG | + | chr4.3:88173303-88173322 | None:intergenic | 35.0% | |
| TTTATGAACTCTTCCTCCAT+TGG | + | chr4.3:88172335-88172354 | None:intergenic | 35.0% | |
| TTTCCACTACAGTAATGCTT+AGG | + | chr4.3:88174443-88174462 | None:intergenic | 35.0% | |
| ! | ACTTTTGGATATGTTGCTCA+AGG | - | chr4.3:88174311-88174330 | MS.gene65402:CDS | 35.0% |
| ! | CAAATACCTTATTTGGACAC+CGG | - | chr4.3:88173377-88173396 | MS.gene65402:CDS | 35.0% |
| ! | CTCTTTGAGGACTTTACAAA+AGG | - | chr4.3:88172253-88172272 | MS.gene65402:CDS | 35.0% |
| ! | GTGGCATTTTGAATTTTCTC+CGG | + | chr4.3:88172890-88172909 | None:intergenic | 35.0% |
| !! | AAAGGTGTCATGAAAAGAGA+AGG | + | chr4.3:88173771-88173790 | None:intergenic | 35.0% |
| !!! | AGCCGTTTTTGAATTCAACT+TGG | - | chr4.3:88173514-88173533 | MS.gene65402:CDS | 35.0% |
| !!! | TTTTTTCTGGCTTTGAACCT+TGG | + | chr4.3:88172490-88172509 | None:intergenic | 35.0% |
| ACAAATGTCACAACCAATGG+AGG | - | chr4.3:88172319-88172338 | MS.gene65402:CDS | 40.0% | |
| ACTTGTCAAATTCAAGGTGC+CGG | - | chr4.3:88172868-88172887 | MS.gene65402:CDS | 40.0% | |
| AGGAAACCAAATGGTTAGCA+TGG | - | chr4.3:88173410-88173429 | MS.gene65402:CDS | 40.0% | |
| ATCACTAACCAGCTTTACAC+CGG | + | chr4.3:88174525-88174544 | None:intergenic | 40.0% | |
| CAAGTCACTAAGGATAGGAT+AGG | - | chr4.3:88172130-88172149 | MS.gene65402:CDS | 40.0% | |
| GCTTCTTGTGGAGTTAAAGT+AGG | + | chr4.3:88173966-88173985 | None:intergenic | 40.0% | |
| GGAAATCCTTCAAGTCACTA+AGG | - | chr4.3:88172120-88172139 | MS.gene65402:CDS | 40.0% | |
| GTGTGATGCTTGTAAGATTC+AGG | - | chr4.3:88173466-88173485 | MS.gene65402:CDS | 40.0% | |
| TACAAACCTGTTGTTGTAGC+TGG | + | chr4.3:88172932-88172951 | None:intergenic | 40.0% | |
| TCCTATCCTTAGTGACTTGA+AGG | + | chr4.3:88172129-88172148 | None:intergenic | 40.0% | |
| TCCTTCAAGTCACTAAGGAT+AGG | - | chr4.3:88172125-88172144 | MS.gene65402:CDS | 40.0% | |
| TCGATCTCTCTCAAATCTAC+AGG | - | chr4.3:88173048-88173067 | MS.gene65402:CDS | 40.0% | |
| TCTTTCTTCGGTTGTTGTAG+TGG | + | chr4.3:88172909-88172928 | None:intergenic | 40.0% | |
| TGAGGACTTTACAAAAGGCA+AGG | - | chr4.3:88172258-88172277 | MS.gene65402:CDS | 40.0% | |
| TGGTTTCCTAAACCATTAGC+CGG | + | chr4.3:88173399-88173418 | None:intergenic | 40.0% | |
| ! | AAAGGCAAGGTACTACTTGT+TGG | - | chr4.3:88172271-88172290 | MS.gene65402:CDS | 40.0% |
| ! | GAGACGGTTTTTCACCATTT+AGG | - | chr4.3:88173828-88173847 | MS.gene65402:intron | 40.0% |
| ! | TCAGATGGTGCCAACATAAA+AGG | - | chr4.3:88172358-88172377 | MS.gene65402:CDS | 40.0% |
| ! | TGGATTGGAGTATGTAGAGT+TGG | + | chr4.3:88173111-88173130 | None:intergenic | 40.0% |
| ! | TTAGGAGGAGCATGATAACA+TGG | + | chr4.3:88174425-88174444 | None:intergenic | 40.0% |
| !! | TGTTGTAGCTGGTCTTTCTT+CGG | + | chr4.3:88172921-88172940 | None:intergenic | 40.0% |
| !!! | CATGACACCTTTTTTCAGCA+AGG | - | chr4.3:88173779-88173798 | MS.gene65402:intron | 40.0% |
| CACAAGCTTCTCTCTCTTTG+AGG | - | chr4.3:88172240-88172259 | MS.gene65402:CDS | 45.0% | |
| CATTAGCCGGTGTCCAAATA+AGG | + | chr4.3:88173386-88173405 | None:intergenic | 45.0% | |
| CCACTACAGTAATGCTTAGG+AGG | + | chr4.3:88174440-88174459 | None:intergenic | 45.0% | |
| CCTCCTAAGCATTACTGTAG+TGG | - | chr4.3:88174437-88174456 | MS.gene65402:CDS | 45.0% | |
| CTCAAGGCTTGGGAAATTTG+AGG | - | chr4.3:88174327-88174346 | MS.gene65402:CDS | 45.0% | |
| CTTCATTCGAAGGGTGGAAA+AGG | + | chr4.3:88173860-88173879 | None:intergenic | 45.0% | |
| GAAAGACCAGCTACAACAAC+AGG | - | chr4.3:88172923-88172942 | MS.gene65402:CDS | 45.0% | |
| GAGAACTCTCTTTCACAAGC+TGG | + | chr4.3:88174395-88174414 | None:intergenic | 45.0% | |
| GGAAAAGGTAGCGTCCTAAA+TGG | + | chr4.3:88173845-88173864 | None:intergenic | 45.0% | |
| GGATATGTTGCTCAAGGCTT+GGG | - | chr4.3:88174317-88174336 | MS.gene65402:CDS | 45.0% | |
| TGGATATGTTGCTCAAGGCT+TGG | - | chr4.3:88174316-88174335 | MS.gene65402:CDS | 45.0% | |
| TTCCAAAAAGGAGACGTCTC+TGG | + | chr4.3:88173546-88173565 | None:intergenic | 45.0% | |
| TTGCAGCCATGCTAACCATT+TGG | + | chr4.3:88173419-88173438 | None:intergenic | 45.0% | |
| TTGTAACCTGCTTACGCATG+AGG | + | chr4.3:88173187-88173206 | None:intergenic | 45.0% | |
| ! | CTTATTTGGACACCGGCTAA+TGG | - | chr4.3:88173384-88173403 | MS.gene65402:CDS | 45.0% |
| ! | TACCAGAGACGTCTCCTTTT+TGG | - | chr4.3:88173541-88173560 | MS.gene65402:CDS | 45.0% |
| !! | CTTGATGGACTCTTAGCTTC+TGG | - | chr4.3:88173150-88173169 | MS.gene65402:intron | 45.0% |
| !! | TTTTTGGTCCGGTGTAAAGC+TGG | - | chr4.3:88174514-88174533 | MS.gene65402:CDS | 45.0% |
| AGTCTCCACGCTTCATTCGA+AGG | + | chr4.3:88173870-88173889 | None:intergenic | 50.0% | |
| ATGATGCCTCATGCGTAAGC+AGG | - | chr4.3:88173178-88173197 | MS.gene65402:intron | 50.0% | |
| CAAGCATCACACGGTTTGTG+AGG | + | chr4.3:88173458-88173477 | None:intergenic | 50.0% | |
| CATAGGTGGTGTGGACCTAA+TGG | - | chr4.3:88173285-88173304 | MS.gene65402:intron | 50.0% | |
| GTCTCCACGCTTCATTCGAA+GGG | + | chr4.3:88173869-88173888 | None:intergenic | 50.0% | |
| GTGGTGTGGACCTAATGGTA+AGG | - | chr4.3:88173290-88173309 | MS.gene65402:intron | 50.0% | |
| ! | GCTTGGGAAATTTGAGGCCA+TGG | - | chr4.3:88174333-88174352 | MS.gene65402:CDS | 50.0% |
| ! | TGGACACCGGCTAATGGTTT+AGG | - | chr4.3:88173390-88173409 | MS.gene65402:CDS | 50.0% |
| TCCACCCTTCGAATGAAGCG+TGG | - | chr4.3:88173862-88173881 | MS.gene65402:intron | 55.0% | |
| TCCACGCTTCATTCGAAGGG+TGG | + | chr4.3:88173866-88173885 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 88172118 | 88174556 | 88172118 | ID=MS.gene65402 |
| chr4.3 | mRNA | 88172118 | 88174556 | 88172118 | ID=MS.gene65402.t1;Parent=MS.gene65402 |
| chr4.3 | exon | 88174269 | 88174556 | 88174269 | ID=MS.gene65402.t1.exon1;Parent=MS.gene65402.t1 |
| chr4.3 | CDS | 88174269 | 88174556 | 88174269 | ID=cds.MS.gene65402.t1;Parent=MS.gene65402.t1 |
| chr4.3 | exon | 88173730 | 88173790 | 88173730 | ID=MS.gene65402.t1.exon2;Parent=MS.gene65402.t1 |
| chr4.3 | CDS | 88173730 | 88173790 | 88173730 | ID=cds.MS.gene65402.t1;Parent=MS.gene65402.t1 |
| chr4.3 | exon | 88173368 | 88173604 | 88173368 | ID=MS.gene65402.t1.exon3;Parent=MS.gene65402.t1 |
| chr4.3 | CDS | 88173368 | 88173604 | 88173368 | ID=cds.MS.gene65402.t1;Parent=MS.gene65402.t1 |
| chr4.3 | exon | 88172118 | 88173127 | 88172118 | ID=MS.gene65402.t1.exon4;Parent=MS.gene65402.t1 |
| chr4.3 | CDS | 88172118 | 88173127 | 88172118 | ID=cds.MS.gene65402.t1;Parent=MS.gene65402.t1 |
| Gene Sequence |
| Protein sequence |
