Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65924.t1 | XP_003628884.2 | 96.5 | 312 | 10 | 1 | 17 | 328 | 20 | 330 | 3.20E-169 | 604.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65924.t1 | P74261 | 31.0 | 281 | 157 | 8 | 48 | 321 | 11 | 261 | 2.8e-20 | 100.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65924.t1 | G7LIJ4 | 96.5 | 312 | 10 | 1 | 17 | 328 | 20 | 330 | 2.3e-169 | 604.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene65924 | 0.810961 | 8.89E-51 | -1.69E-46 |
| MS.gene050747 | MS.gene65924 | 0.805714 | 1.17E-49 | -1.69E-46 |
| MS.gene051666 | MS.gene65924 | 0.81025 | 1.27E-50 | -1.69E-46 |
| MS.gene051694 | MS.gene65924 | 0.804433 | 2.17E-49 | -1.69E-46 |
| MS.gene052481 | MS.gene65924 | 0.808335 | 3.26E-50 | -1.69E-46 |
| MS.gene053107 | MS.gene65924 | 0.810372 | 1.19E-50 | -1.69E-46 |
| MS.gene056796 | MS.gene65924 | 0.822198 | 2.69E-53 | -1.69E-46 |
| MS.gene057011 | MS.gene65924 | 0.810898 | 9.17E-51 | -1.69E-46 |
| MS.gene057196 | MS.gene65924 | 0.802118 | 6.53E-49 | -1.69E-46 |
| MS.gene057658 | MS.gene65924 | 0.809673 | 1.68E-50 | -1.69E-46 |
| MS.gene058444 | MS.gene65924 | 0.805609 | 1.23E-49 | -1.69E-46 |
| MS.gene058544 | MS.gene65924 | 0.809246 | 2.08E-50 | -1.69E-46 |
| MS.gene059023 | MS.gene65924 | 0.819668 | 1.03E-52 | -1.69E-46 |
| MS.gene059536 | MS.gene65924 | 0.803135 | 4.03E-49 | -1.69E-46 |
| MS.gene060675 | MS.gene65924 | 0.827334 | 1.66E-54 | -1.69E-46 |
| MS.gene06092 | MS.gene65924 | 0.852735 | 3.80E-61 | -1.69E-46 |
| MS.gene061150 | MS.gene65924 | 0.837971 | 3.79E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65924.t1 | MTR_8g068220 | 96.474 | 312 | 10 | 1 | 17 | 328 | 20 | 330 | 0.0 | 614 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65924.t1 | AT4G38020 | 66.904 | 281 | 89 | 2 | 48 | 324 | 69 | 349 | 6.67e-138 | 395 |
Find 66 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAAATATTATTTGTTCTT+TGG | 0.214392 | 8.4:+35057613 | None:intergenic |
| CATGGTTCTGTTCTTGTTGT+TGG | 0.254929 | 8.4:-35061769 | MS.gene65924:CDS |
| GCACAATGGTGTATCTAATA+TGG | 0.255504 | 8.4:+35057774 | None:intergenic |
| TTTAGTCCTTGATGGAATTC+AGG | 0.283941 | 8.4:-35060088 | MS.gene65924:intron |
| TTTCAACTCCCTATAGTTTC+CGG | 0.307983 | 8.4:-35057916 | MS.gene65924:CDS |
| TTGGGAATTTCAGCCTTATC+AGG | 0.317783 | 8.4:+35060294 | None:intergenic |
| TCACAAGCTCACATGCTTGC+AGG | 0.336171 | 8.4:+35057710 | None:intergenic |
| TCACTAAATGTTTCAGTTGC+AGG | 0.341156 | 8.4:-35057661 | MS.gene65924:CDS |
| TTGTTTAATTCTTCCTGATA+AGG | 0.346817 | 8.4:-35060307 | MS.gene65924:CDS |
| TTCCGGAAGTTGGAATCATT+TGG | 0.348466 | 8.4:-35057899 | MS.gene65924:CDS |
| TTCCAAATGAAGTTGCTTGC+TGG | 0.349311 | 8.4:-35057859 | MS.gene65924:CDS |
| TTCCAAATGATTCCAACTTC+CGG | 0.350690 | 8.4:+35057897 | None:intergenic |
| GCCCAGCAAGCAACTTCATT+TGG | 0.353611 | 8.4:+35057857 | None:intergenic |
| TCCCTATAGTTTCCGGAAGT+TGG | 0.354050 | 8.4:-35057909 | MS.gene65924:CDS |
| TTGTTGGCACTACACCTATC+AGG | 0.368135 | 8.4:-35061753 | MS.gene65924:intron |
| GCGCAGATGGAAACCACTTC+TGG | 0.375633 | 8.4:+35060118 | None:intergenic |
| TTGAGACAGTGTTTCACAAA+TGG | 0.395579 | 8.4:+35061823 | None:intergenic |
| ATAGTGATGAGAAAGATTTC+AGG | 0.397455 | 8.4:-35060228 | MS.gene65924:CDS |
| TCCAACTTCCGGAAACTATA+GGG | 0.404665 | 8.4:+35057908 | None:intergenic |
| TGAAATCTTTCTCATCACTA+TGG | 0.405370 | 8.4:+35060230 | None:intergenic |
| CGGAGTGCTTTCTCGTTGAA+TGG | 0.407160 | 8.4:+35057958 | None:intergenic |
| TTCCAACTTCCGGAAACTAT+AGG | 0.407679 | 8.4:+35057907 | None:intergenic |
| GATCAGCTGTGGCCTTCAAA+TGG | 0.421077 | 8.4:-35059158 | MS.gene65924:CDS |
| TCCAAATGAAGTTGCTTGCT+GGG | 0.428331 | 8.4:-35057858 | MS.gene65924:CDS |
| GGAGAAACCGAAAGTCTTGT+TGG | 0.456282 | 8.4:+35061919 | None:intergenic |
| AATGAAAATTAGAACCTGAT+AGG | 0.464064 | 8.4:+35061739 | None:intergenic |
| GTGGATTCATTCAACCCATT+GGG | 0.465000 | 8.4:+35060276 | None:intergenic |
| TGAGACAGTGTTTCACAAAT+GGG | 0.466462 | 8.4:+35061824 | None:intergenic |
| AGCAATGTACCGAGGTTACC+TGG | 0.478339 | 8.4:+35059183 | None:intergenic |
| AATTGTTCACTGCAGGATCC+AGG | 0.480049 | 8.4:-35059201 | MS.gene65924:intron |
| TCTTCTTATCGTCGCTCTCA+TGG | 0.487765 | 8.4:-35061787 | MS.gene65924:CDS |
| ATTAGATACACCATTGTGCT+TGG | 0.489224 | 8.4:-35057770 | MS.gene65924:CDS |
| CACAAGCTCACATGCTTGCA+GGG | 0.489262 | 8.4:+35057711 | None:intergenic |
| AGGATGCGCCTCGGCTAGCT+CGG | 0.489834 | 8.4:+35057938 | None:intergenic |
| ATAATAACTTACCCATTTGA+AGG | 0.490906 | 8.4:+35059146 | None:intergenic |
| ATCAGCTGTGGCCTTCAAAT+GGG | 0.491246 | 8.4:-35059157 | MS.gene65924:intron |
| TTATTTACAGAGAAATCTAC+AGG | 0.495436 | 8.4:-35060368 | MS.gene65924:intron |
| AGTGGATTCATTCAACCCAT+TGG | 0.495802 | 8.4:+35060275 | None:intergenic |
| ACTAAAATCCGATGCGCAGA+TGG | 0.499079 | 8.4:+35060105 | None:intergenic |
| ATCACAGAAGCTTTGAGAAA+GGG | 0.502364 | 8.4:+35057798 | None:intergenic |
| GAGAAACCGAAAGTCTTGTT+GGG | 0.505812 | 8.4:+35061920 | None:intergenic |
| CTGGGCATCCACAGCATGAA+GGG | 0.525650 | 8.4:-35057840 | MS.gene65924:CDS |
| CTTTGAGAAAGGGAACAAAC+CGG | 0.534121 | 8.4:+35057808 | None:intergenic |
| AATCACAGAAGCTTTGAGAA+AGG | 0.539971 | 8.4:+35057797 | None:intergenic |
| TTGAATGGATCACAGCATCC+AGG | 0.550903 | 8.4:+35057973 | None:intergenic |
| ATCAAAAGCACTGCCAGAAG+TGG | 0.554660 | 8.4:-35060131 | MS.gene65924:CDS |
| ATTCGTTGCTCTTGTTGTTG+TGG | 0.555474 | 8.4:+35061895 | None:intergenic |
| GCTGGGCATCCACAGCATGA+AGG | 0.558043 | 8.4:-35057841 | MS.gene65924:CDS |
| TTGATTAACCCTTCATGCTG+TGG | 0.573547 | 8.4:+35057832 | None:intergenic |
| ATAAGGCTGAAATTCCCAAT+GGG | 0.575863 | 8.4:-35060290 | MS.gene65924:CDS |
| GCATGAAGGGTTAATCAAGC+CGG | 0.582434 | 8.4:-35057827 | MS.gene65924:CDS |
| AGTGGTTTCCATCTGCGCAT+CGG | 0.584870 | 8.4:-35060113 | MS.gene65924:CDS |
| CGTTGCTCTTGTTGTTGTGG+CGG | 0.599233 | 8.4:+35061898 | None:intergenic |
| CTTGTGAGCATTGCAATGAC+AGG | 0.603906 | 8.4:-35057694 | MS.gene65924:CDS |
| GAGTCACCTGAATTCCATCA+AGG | 0.604011 | 8.4:+35060082 | None:intergenic |
| CTAAATGTTTCAGTTGCAGG+TGG | 0.604208 | 8.4:-35057658 | MS.gene65924:CDS |
| GATAAGGCTGAAATTCCCAA+TGG | 0.606196 | 8.4:-35060291 | MS.gene65924:CDS |
| TACATTGCTCAGATCAGCTG+TGG | 0.607696 | 8.4:-35059169 | MS.gene65924:CDS |
| AAAGCACTCCGAGCTAGCCG+AGG | 0.621443 | 8.4:-35057946 | MS.gene65924:CDS |
| GGAGTTGAAAGGATGCGCCT+CGG | 0.626018 | 8.4:+35057929 | None:intergenic |
| GACACGCACAATAGAATCAG+TGG | 0.627366 | 8.4:+35060257 | None:intergenic |
| GGAAACTATAGGGAGTTGAA+AGG | 0.629169 | 8.4:+35057918 | None:intergenic |
| CTGCAGGATCCAGGTAACCT+CGG | 0.657699 | 8.4:-35059192 | MS.gene65924:intron |
| CTGCCTAAAACCAAGCACAA+TGG | 0.679001 | 8.4:+35057760 | None:intergenic |
| CTGATCTGAGCAATGTACCG+AGG | 0.714931 | 8.4:+35059175 | None:intergenic |
| AATGACGTGTCATCGATGTG+TGG | 0.740046 | 8.4:-35064930 | MS.gene65924:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 35057621 | 35064963 | 35057621 | ID=MS.gene65924 |
| chr8.4 | mRNA | 35057621 | 35064963 | 35057621 | ID=MS.gene65924.t1;Parent=MS.gene65924 |
| chr8.4 | exon | 35064917 | 35064963 | 35064917 | ID=MS.gene65924.t1.exon1;Parent=MS.gene65924.t1 |
| chr8.4 | CDS | 35064917 | 35064963 | 35064917 | ID=cds.MS.gene65924.t1;Parent=MS.gene65924.t1 |
| chr8.4 | exon | 35061754 | 35061957 | 35061754 | ID=MS.gene65924.t1.exon2;Parent=MS.gene65924.t1 |
| chr8.4 | CDS | 35061754 | 35061957 | 35061754 | ID=cds.MS.gene65924.t1;Parent=MS.gene65924.t1 |
| chr8.4 | exon | 35060089 | 35060380 | 35060089 | ID=MS.gene65924.t1.exon3;Parent=MS.gene65924.t1 |
| chr8.4 | CDS | 35060089 | 35060380 | 35060089 | ID=cds.MS.gene65924.t1;Parent=MS.gene65924.t1 |
| chr8.4 | exon | 35059158 | 35059208 | 35059158 | ID=MS.gene65924.t1.exon4;Parent=MS.gene65924.t1 |
| chr8.4 | CDS | 35059158 | 35059208 | 35059158 | ID=cds.MS.gene65924.t1;Parent=MS.gene65924.t1 |
| chr8.4 | exon | 35057621 | 35058013 | 35057621 | ID=MS.gene65924.t1.exon5;Parent=MS.gene65924.t1 |
| chr8.4 | CDS | 35057621 | 35058013 | 35057621 | ID=cds.MS.gene65924.t1;Parent=MS.gene65924.t1 |
| Gene Sequence |
| Protein sequence |
