AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66171

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66171.t1 MTR_3g056110 96.310 542 20 0 1 542 1 542 0.0 1089
MS.gene66171.t1 MTR_5g094450 67.577 549 154 9 1 542 1 532 0.0 731
MS.gene66171.t1 MTR_5g097480 57.713 551 217 6 1 542 1 544 0.0 651
MS.gene66171.t1 MTR_4g133660 48.663 561 249 9 1 541 1 542 5.91e-178 513
MS.gene66171.t1 MTR_2g082090 58.602 372 154 0 170 541 207 578 1.79e-168 489
MS.gene66171.t1 MTR_2g026250 46.568 539 231 7 56 542 63 596 4.07e-165 482
MS.gene66171.t1 MTR_1g029420 50.000 370 185 0 173 542 223 592 5.40e-141 420
MS.gene66171.t1 MTR_3g089055 49.081 381 193 1 162 542 186 565 2.70e-137 409
MS.gene66171.t1 MTR_3g089055 49.081 381 193 1 162 542 191 570 3.30e-137 409
MS.gene66171.t1 MTR_7g027190 34.120 551 295 13 21 542 162 673 7.25e-79 261
MS.gene66171.t1 MTR_3g065980 35.219 389 217 11 172 542 169 540 2.16e-62 214
MS.gene66171.t1 MTR_4g064160 30.822 438 287 8 114 541 252 683 3.95e-62 216
MS.gene66171.t1 MTR_8g442410 32.292 384 239 7 171 542 151 525 8.03e-62 212
MS.gene66171.t1 MTR_7g062120 30.319 376 255 4 171 542 368 740 6.82e-61 214
MS.gene66171.t1 MTR_4g064150 32.540 378 246 6 171 541 357 732 1.63e-60 213
MS.gene66171.t1 MTR_2g097390 28.980 490 311 12 77 541 163 640 1.77e-59 208
MS.gene66171.t1 MTR_6g047750 37.464 347 188 8 210 542 331 662 6.90e-59 207
MS.gene66171.t1 MTR_7g057230 34.897 341 208 6 208 541 323 656 7.21e-59 207
MS.gene66171.t1 MTR_2g097350 28.994 507 311 14 61 541 156 639 1.98e-58 205
MS.gene66171.t1 MTR_2g097410 31.496 381 244 8 171 541 367 740 2.38e-58 207
MS.gene66171.t1 MTR_2g097310 29.385 439 285 7 122 541 205 637 8.63e-58 204
MS.gene66171.t1 MTR_4g064120 31.016 374 253 3 171 539 250 623 7.59e-57 201
MS.gene66171.t1 MTR_4g064180 31.635 373 250 3 171 538 250 622 1.97e-56 200
MS.gene66171.t1 MTR_7g074650 33.973 365 226 6 179 542 442 792 6.12e-56 201
MS.gene66171.t1 MTR_4g064200 32.000 375 246 7 171 541 280 649 2.69e-55 197
MS.gene66171.t1 MTR_4g104020 29.427 384 249 5 172 542 146 520 7.16e-55 193
MS.gene66171.t1 MTR_4g097080 29.114 395 253 9 163 542 121 503 3.82e-54 191
MS.gene66171.t1 MTR_2g097467 29.177 401 277 4 142 541 259 653 2.23e-52 189
MS.gene66171.t1 MTR_3g022830 27.578 417 246 5 176 542 26 436 2.25e-52 184
MS.gene66171.t1 MTR_4g077760 35.028 354 213 10 195 542 213 555 2.29e-50 182
MS.gene66171.t1 MTR_4g102790 30.769 390 240 11 169 541 285 661 3.01e-49 181
MS.gene66171.t1 MTR_2g097463 29.839 372 257 2 171 541 286 654 4.61e-49 180
MS.gene66171.t1 MTR_2g097473 30.027 373 255 5 171 541 285 653 2.58e-48 178
MS.gene66171.t1 MTR_4g076020 32.432 370 216 10 179 542 88 429 3.07e-47 171
MS.gene66171.t1 MTR_2g097380 26.582 474 299 12 77 541 127 560 5.83e-47 172
MS.gene66171.t1 MTR_5g015490 29.156 391 240 12 175 542 114 490 1.54e-46 170
MS.gene66171.t1 MTR_1g096030 29.442 394 238 11 175 542 80 459 6.40e-44 162
MS.gene66171.t1 MTR_1g069725 30.769 377 236 12 171 541 105 462 1.02e-42 159
MS.gene66171.t1 MTR_5g058860 31.421 366 224 11 194 541 140 496 6.80e-39 149
MS.gene66171.t1 MTR_3g072710 30.108 372 223 10 194 541 141 499 7.28e-39 149
MS.gene66171.t1 MTR_2g089100 25.118 422 272 9 154 541 31 442 2.85e-38 146
MS.gene66171.t1 MTR_2g099110 27.551 392 248 10 174 542 73 451 1.21e-35 139
MS.gene66171.t1 MTR_2g034250 27.506 389 254 9 172 542 207 585 1.41e-34 138
MS.gene66171.t1 MTR_5g015950 24.824 427 251 11 172 541 32 445 1.59e-32 130
MS.gene66171.t1 MTR_3g053270 24.824 427 251 11 172 541 32 445 1.59e-32 130
MS.gene66171.t1 MTR_4g095500 24.586 423 270 9 166 541 47 467 3.18e-32 129
MS.gene66171.t1 MTR_0092s0100 26.933 375 251 8 174 542 371 728 1.75e-31 129
MS.gene66171.t1 MTR_0092s0100 26.933 375 251 8 174 542 374 731 2.01e-31 129
MS.gene66171.t1 MTR_2g034280 25.781 384 264 9 172 542 200 575 9.13e-31 126
MS.gene66171.t1 MTR_7g109580 24.672 381 267 9 171 542 185 554 7.80e-29 120
MS.gene66171.t1 MTR_4g076140 30.045 223 148 3 160 380 32 248 1.92e-28 118
MS.gene66171.t1 MTR_4g076140 37.589 141 87 1 403 542 329 469 4.13e-26 111
MS.gene66171.t1 MTR_4g026485 25.000 384 246 12 174 542 268 624 1.95e-28 120
MS.gene66171.t1 MTR_2g034260 25.369 339 236 6 172 499 207 539 4.99e-28 118
MS.gene66171.t1 MTR_1g086970 26.062 353 232 12 206 542 139 478 2.73e-26 112
MS.gene66171.t1 MTR_5g009080 28.302 212 145 2 172 382 53 258 4.59e-26 111
MS.gene66171.t1 MTR_5g009080 34.752 141 91 1 403 542 338 478 3.64e-23 103
MS.gene66171.t1 MTR_8g093070 28.212 397 246 17 160 541 123 495 5.48e-26 111
MS.gene66171.t1 MTR_8g020840 25.062 403 246 15 173 542 169 548 1.00e-23 105
MS.gene66171.t1 MTR_8g077940 24.485 388 253 14 171 542 178 541 1.23e-17 86.3
MS.gene66171.t1 MTR_3g022580 30.894 123 84 1 420 542 62 183 1.57e-14 72.4
MS.gene66171.t1 MTR_3g022005 35.484 93 60 0 450 542 33 125 2.63e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66171.t1 AT1G50600 55.861 546 217 7 1 542 72 597 0.0 590
MS.gene66171.t1 AT1G50600 55.861 546 217 7 1 542 76 601 0.0 590
MS.gene66171.t1 AT1G50600 55.861 546 217 7 1 542 1 526 0.0 589
MS.gene66171.t1 AT5G48150 64.319 426 149 2 118 542 67 490 0.0 578
MS.gene66171.t1 AT5G48150 64.319 426 149 2 118 542 67 490 0.0 578
MS.gene66171.t1 AT5G48150 64.319 426 149 2 118 542 67 490 0.0 578
MS.gene66171.t1 AT5G48150 64.319 426 149 2 118 542 67 490 0.0 578
MS.gene66171.t1 AT2G04890 58.937 414 163 1 129 542 7 413 0.0 517
MS.gene66171.t1 AT4G17230 49.183 551 245 10 1 542 1 525 8.32e-171 494
MS.gene66171.t1 AT4G17230 49.183 551 245 10 1 542 1 525 8.32e-171 494
MS.gene66171.t1 AT1G21450 42.430 502 265 5 63 542 94 593 2.85e-136 408
MS.gene66171.t1 AT1G66350 36.267 375 215 10 172 542 152 506 2.35e-67 226
MS.gene66171.t1 AT5G17490 35.809 377 219 10 172 542 157 516 2.03e-66 224
MS.gene66171.t1 AT5G52510 37.931 348 191 7 210 542 303 640 2.21e-66 227
MS.gene66171.t1 AT1G07530 33.690 374 246 2 170 541 391 764 9.35e-66 228
MS.gene66171.t1 AT1G14920 35.171 381 217 10 172 542 169 529 7.67e-65 220
MS.gene66171.t1 AT3G03450 34.933 375 231 8 172 542 180 545 1.04e-63 218
MS.gene66171.t1 AT2G01570 35.638 376 222 9 172 542 221 581 6.42e-63 216
MS.gene66171.t1 AT2G29060 33.067 375 247 3 171 541 280 654 2.08e-62 216
MS.gene66171.t1 AT2G29060 33.067 375 247 3 171 541 317 691 3.49e-62 217
MS.gene66171.t1 AT5G59450 28.191 376 266 3 171 542 223 598 7.26e-54 192
MS.gene66171.t1 AT5G66770 34.218 377 233 9 172 542 217 584 8.21e-54 192
MS.gene66171.t1 AT3G46600 30.108 372 258 2 172 542 1 371 8.68e-54 186
MS.gene66171.t1 AT3G46600 30.295 373 258 2 171 542 208 579 1.45e-53 191
MS.gene66171.t1 AT3G46600 30.295 373 258 2 171 542 176 547 2.31e-53 190
MS.gene66171.t1 AT2G29065 31.332 383 248 8 171 541 248 627 1.47e-52 189
MS.gene66171.t1 AT3G54220 30.993 413 266 8 134 541 251 649 2.09e-52 189
MS.gene66171.t1 AT3G50650 34.986 363 218 7 189 542 189 542 3.53e-52 186
MS.gene66171.t1 AT1G50420 27.483 433 245 8 172 542 54 479 8.14e-52 184
MS.gene66171.t1 AT2G37650 28.989 376 258 5 171 542 331 701 1.22e-51 188
MS.gene66171.t1 AT2G37650 28.989 376 258 5 171 542 343 713 1.88e-51 187
MS.gene66171.t1 AT1G07520 29.676 401 269 7 150 541 132 528 9.55e-50 179
MS.gene66171.t1 AT1G07520 29.676 401 269 7 150 541 132 528 9.55e-50 179
MS.gene66171.t1 AT5G41920 32.637 383 224 11 172 542 40 400 1.56e-49 176
MS.gene66171.t1 AT1G07520 29.777 403 266 8 150 541 296 692 3.25e-49 181
MS.gene66171.t1 AT1G07520 29.777 403 266 8 150 541 297 693 3.38e-49 181
MS.gene66171.t1 AT4G37650 27.525 396 247 11 175 542 146 529 2.36e-44 165
MS.gene66171.t1 AT1G55580 28.019 414 246 9 172 542 41 445 1.23e-41 155
MS.gene66171.t1 AT1G63100 30.334 389 245 7 171 541 248 628 1.74e-41 158
MS.gene66171.t1 AT1G63100 30.334 389 245 7 171 541 273 653 2.46e-41 158
MS.gene66171.t1 AT3G49950 25.000 396 256 9 172 541 27 407 1.38e-35 138
MS.gene66171.t1 AT4G08250 29.085 306 201 6 244 541 179 476 8.38e-33 131
MS.gene66171.t1 AT3G60630 28.477 302 189 9 254 542 335 622 5.78e-25 109
MS.gene66171.t1 AT2G45160 25.281 356 218 13 207 542 312 639 1.50e-23 105
MS.gene66171.t1 AT4G00150 24.808 391 233 16 172 542 205 554 2.03e-22 101
MS.gene66171.t1 AT3G13840 24.346 382 260 11 173 542 146 510 1.09e-21 99.0
MS.gene66171.t1 AT4G36710 24.810 395 244 14 171 542 121 485 5.30e-20 93.6
MS.gene66171.t1 AT5G67411 29.082 196 122 6 295 479 9 198 1.29e-14 73.2

Find 116 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACCTGTTATTCTAATCTT+TGG 0.235841 3.3:+45713474 None:intergenic
CGCAAAATAGTATCCGTTTC+AGG 0.240168 3.3:-45713801 MS.gene66171:CDS
CTGCAAGAACGGAGCGTTAT+TGG 0.252017 3.3:+45713146 None:intergenic
ATCTGCTTCGTTCTTATTAG+AGG 0.270897 3.3:-45713949 MS.gene66171:CDS
CGTGAGTAGAGGGAGAATTT+TGG 0.279803 3.3:+45714177 None:intergenic
CAGGCTCTTGCTCGCCGGCC+TGG 0.315935 3.3:-45713504 MS.gene66171:CDS
CGATTTGTAGATTGTACTTC+CGG 0.341558 3.3:+45713716 None:intergenic
ATCTTATTGTTTCTTGTGCT+TGG 0.342571 3.3:-45712795 MS.gene66171:CDS
AGCAGATTCTTCTTGAACTT+TGG 0.342587 3.3:+45713965 None:intergenic
CCTCCAAAGATTAGAATAAC+AGG 0.365174 3.3:-45713477 MS.gene66171:CDS
GATGAGAAGGATAGAATGTT+TGG 0.365693 3.3:+45714261 None:intergenic
GTTTCGAAGATTGCCTCTTC+CGG 0.372158 3.3:-45713735 MS.gene66171:CDS
ATATGTCCGTACTTTAAATT+CGG 0.373248 3.3:-45713633 MS.gene66171:CDS
AGAGATGGTGCACTGTTTCT+TGG 0.374714 3.3:-45712832 MS.gene66171:CDS
CAGGCCGGCGAGCAAGAGCC+TGG 0.383296 3.3:+45713505 None:intergenic
CATTCACTTGAATTTGTCAA+TGG 0.402839 3.3:-45714110 MS.gene66171:CDS
TTCACCGCCACAGCTTCATT+AGG 0.419761 3.3:+45713297 None:intergenic
TAATGTCGAACAGCACTGTT+TGG 0.423158 3.3:-45713040 MS.gene66171:CDS
GTAGAACCTGGACTATTGTT+AGG 0.425781 3.3:+45714146 None:intergenic
CTTGATTGAAGAATTGATGA+AGG 0.426007 3.3:+45712897 None:intergenic
ATCCTCTCTTTGTGATCCCT+TGG 0.427208 3.3:+45713063 None:intergenic
AACATCAATTGATTCATAAA+CGG 0.428207 3.3:+45713092 None:intergenic
CAGAAACATGAAATTTCTTA+CGG 0.431673 3.3:-45714383 MS.gene66171:CDS
CCTGTTATTCTAATCTTTGG+AGG 0.435815 3.3:+45713477 None:intergenic
GCTCACTCATCTTATGCCTT+AGG 0.446845 3.3:+45714039 None:intergenic
TCATCGTATGCCCGCGGTGG+GGG 0.447317 3.3:-45713438 MS.gene66171:CDS
CAATTGATGTTGTTCTTCCA+AGG 0.452748 3.3:-45713080 MS.gene66171:CDS
TTCGGATATATGTCTGCAAA+TGG 0.452855 3.3:-45713615 MS.gene66171:CDS
GGCGAGCAAGAGCCTGGATT+AGG 0.464253 3.3:+45713511 None:intergenic
ATGAAGGAGCTCAATGGATA+AGG 0.465572 3.3:+45712913 None:intergenic
AGGCAGTCCAATCGAACGAT+TGG 0.470118 3.3:-45713781 MS.gene66171:CDS
TTGTCTAGATTCTCAATTGA+GGG 0.471983 3.3:+45714308 None:intergenic
TGCTTGTTGAAGCAGATTCA+AGG 0.473275 3.3:+45714216 None:intergenic
CTAATCTTTGGAGGTCCTCC+AGG 0.475160 3.3:+45713486 None:intergenic
TGAATGGTCACACTTTCATC+TGG 0.476627 3.3:+45713249 None:intergenic
TTCGATTGGACTGCCTGAAA+CGG 0.476643 3.3:+45713788 None:intergenic
TGGTACATGATGTAGCATAA+TGG 0.479324 3.3:+45713269 None:intergenic
AAATTTCTTACGGCTCTGAC+AGG 0.480661 3.3:-45714373 MS.gene66171:CDS
GTAGATTGTACTTCCGGAAG+AGG 0.481683 3.3:+45713722 None:intergenic
CAATTTGAAAATCAATTATG+TGG 0.486415 3.3:+45713556 None:intergenic
CTAACAGCATGGAACTCAAA+TGG 0.487453 3.3:+45713363 None:intergenic
TGTTCTAGAGTTTGATGAGA+AGG 0.490850 3.3:+45714248 None:intergenic
TCAACTAGAGTCACCACCTT+AGG 0.493149 3.3:+45713183 None:intergenic
ATTCATCGTATGCCCGCGGT+GGG 0.494640 3.3:-45713440 MS.gene66171:CDS
GGTGAGAAACCGTGAGTAGA+GGG 0.496435 3.3:+45714167 None:intergenic
AAGTAAGAATCCTCCGCCTC+AGG 0.498080 3.3:+45714083 None:intergenic
ATCTCTAGCATCTCTACGTT+AGG 0.500658 3.3:+45713996 None:intergenic
GTCATGAGCTTCTGAGTAAG+TGG 0.500715 3.3:-45712966 MS.gene66171:CDS
ATGGTGCACTGTTTCTTGGT+TGG 0.503499 3.3:-45712828 MS.gene66171:CDS
CAATGGTTCACCTGAGGCGG+AGG 0.504414 3.3:-45714093 MS.gene66171:CDS
TACGCTCCCAATCGTTCGAT+TGG 0.508907 3.3:+45713774 None:intergenic
CAAAATTCTCCCTCTACTCA+CGG 0.512747 3.3:-45714176 MS.gene66171:CDS
GGCAGTCCAATCGAACGATT+GGG 0.513943 3.3:-45713780 MS.gene66171:CDS
TCAGTGGGTGAGCCTAATCC+AGG 0.518047 3.3:-45713523 MS.gene66171:CDS
TCTTCGAGTCGCACCTCAGA+AGG 0.519582 3.3:+45713333 None:intergenic
GTGCACCATCTCTCTCTGCT+AGG 0.520562 3.3:+45712842 None:intergenic
TTCATCGTATGCCCGCGGTG+GGG 0.523230 3.3:-45713439 MS.gene66171:CDS
TTCTCACCTAACAATAGTCC+AGG 0.523328 3.3:-45714152 MS.gene66171:CDS
TTAAAATGCAGCGAGCCTAC+TGG 0.529023 3.3:-45713693 MS.gene66171:CDS
AATTGATGTTGTTCTTCCAA+GGG 0.531808 3.3:-45713079 MS.gene66171:CDS
TGCACCATCTCTCTCTGCTA+GGG 0.537219 3.3:+45712843 None:intergenic
ACATGGAGATGGTATCTAGA+GGG 0.537613 3.3:-45713908 MS.gene66171:CDS
TCTTATGCCTTAGGTCATCA+TGG 0.538147 3.3:+45714048 None:intergenic
ATTGTCTAGATTCTCAATTG+AGG 0.540884 3.3:+45714307 None:intergenic
TGTCAATGGTTCACCTGAGG+CGG 0.541775 3.3:-45714096 MS.gene66171:CDS
ATAGTCCAGGTTCTACGTTA+AGG 0.542222 3.3:-45714139 MS.gene66171:CDS
AGCCATCGCGGAAGCTATGA+AGG 0.542662 3.3:-45713592 MS.gene66171:CDS
TCGTGACAGGTTGCTGCGAT+TGG 0.544162 3.3:-45713220 MS.gene66171:CDS
GGCTAAGCACATGTCTCCTA+AGG 0.546864 3.3:-45713199 MS.gene66171:CDS
TCGACGAAACGCTGCAAGAA+CGG 0.547227 3.3:+45713135 None:intergenic
TAATCCAGGCTCTTGCTCGC+CGG 0.552248 3.3:-45713509 MS.gene66171:CDS
CTCCTTCATAGCTTCCGCGA+TGG 0.552554 3.3:+45713590 None:intergenic
CCATTCATAATCATCGTGAC+AGG 0.553760 3.3:-45713233 MS.gene66171:CDS
TGAACTTAGTAGCTTGCGAA+GGG 0.564647 3.3:-45713005 MS.gene66171:CDS
CCTGTCACGATGATTATGAA+TGG 0.565547 3.3:+45713233 None:intergenic
ATCATGACCATGATGACCTA+AGG 0.567502 3.3:-45714055 MS.gene66171:CDS
TTCATAAAGTACATGCATGT+AGG 0.567692 3.3:+45713656 None:intergenic
ACGATTGGGAGCGTACATGT+TGG 0.575556 3.3:-45713766 MS.gene66171:CDS
TATCGAGACAATTGAATGGA+TGG 0.577277 3.3:-45713835 MS.gene66171:CDS
AGGTGAGAAACCGTGAGTAG+AGG 0.577735 3.3:+45714166 None:intergenic
AAGGCATAAGATGAGTGAGC+TGG 0.578063 3.3:-45714036 MS.gene66171:CDS
ATGATATCGAGACAATTGAA+TGG 0.578703 3.3:-45713839 MS.gene66171:CDS
GTGAACTTAGTAGCTTGCGA+AGG 0.579196 3.3:-45713006 MS.gene66171:CDS
TCTTATTAGAGGCAGAGAAG+TGG 0.579922 3.3:-45713938 MS.gene66171:CDS
ATTGGGAGCGTACATGTTGG+AGG 0.587024 3.3:-45713763 MS.gene66171:CDS
GCTCTTGCTCGCCGGCCTGG+AGG 0.588420 3.3:-45713501 MS.gene66171:CDS
TATTCATCGTATGCCCGCGG+TGG 0.589109 3.3:-45713441 MS.gene66171:CDS
GAGCTGGAATCTGTTATGCT+CGG 0.590793 3.3:-45714020 MS.gene66171:CDS
TTCCAAGGGATCACAAAGAG+AGG 0.599204 3.3:-45713065 MS.gene66171:CDS
TGCGGCCTTAACGTAGAACC+TGG 0.605445 3.3:+45714134 None:intergenic
TACGACTCGAGATTCTGTAC+AGG 0.608834 3.3:+45714338 None:intergenic
CTGCTAGGGTGTAATGTCCA+CGG 0.609288 3.3:+45712857 None:intergenic
TGTTAGAGTGTATCCTTCTG+AGG 0.611616 3.3:-45713346 MS.gene66171:CDS
AACATGGAGATGGTATCTAG+AGG 0.612916 3.3:-45713909 MS.gene66171:CDS
TCTTTGGAGGTCCTCCAGGC+CGG 0.613618 3.3:+45713490 None:intergenic
TTATACTTGTGCAAAAGCAG+TGG 0.618641 3.3:-45713868 MS.gene66171:CDS
AAGGATACACTCTAACAGCA+TGG 0.621994 3.3:+45713352 None:intergenic
GAATTGATGAAGGAGCTCAA+TGG 0.624450 3.3:+45712907 None:intergenic
AATCTTCTCGAGAGCTACCG+TGG 0.627998 3.3:-45712874 MS.gene66171:CDS
TCGTCCTAATGAAGCTGTGG+CGG 0.629870 3.3:-45713301 MS.gene66171:CDS
ATTTGTCAATGGTTCACCTG+AGG 0.630937 3.3:-45714099 MS.gene66171:CDS
ATATATCCGAATTTAAAGTA+CGG 0.633125 3.3:+45713627 None:intergenic
ACAAATTCAAGTGAATGTTG+CGG 0.633662 3.3:+45714116 None:intergenic
CATGGAGATGGTATCTAGAG+GGG 0.636479 3.3:-45713907 MS.gene66171:CDS
TGTTTGGCAGCTGTTATCAG+AGG 0.643018 3.3:+45714277 None:intergenic
GAAAGCAGTTCATTACCAGT+AGG 0.643103 3.3:+45713678 None:intergenic
AATTGGACAAGGAACTCAGT+GGG 0.647751 3.3:-45713538 MS.gene66171:CDS
TAAGCACATGTCTCCTAAGG+TGG 0.649063 3.3:-45713196 MS.gene66171:CDS
ACTTCGTCCTAATGAAGCTG+TGG 0.652136 3.3:-45713304 MS.gene66171:CDS
TTGCGAAGGGGAAGAAAGAG+TGG 0.653404 3.3:-45712992 MS.gene66171:CDS
GTCTGCAAATGGAGCCATCG+CGG 0.659747 3.3:-45713604 MS.gene66171:CDS
TACACCCTAGCAGAGAGAGA+TGG 0.671905 3.3:-45712847 MS.gene66171:CDS
GAACTTAGTAGCTTGCGAAG+GGG 0.697810 3.3:-45713004 MS.gene66171:CDS
CATCGCGGAAGCTATGAAGG+AGG 0.707945 3.3:-45713589 MS.gene66171:CDS
TCTTATTCATCGTATGCCCG+CGG 0.710163 3.3:-45713444 MS.gene66171:CDS
AAATTGGACAAGGAACTCAG+TGG 0.723091 3.3:-45713539 MS.gene66171:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTTGAAAATCAATTATG+TGG + chr3.3:45713631-45713650 None:intergenic 20.0%
!!! AACATCAATTGATTCATAAA+CGG + chr3.3:45714095-45714114 None:intergenic 20.0%
!!! ATATATCCGAATTTAAAGTA+CGG + chr3.3:45713560-45713579 None:intergenic 20.0%
!!! CACATAATTGATTTTCAAAT+TGG - chr3.3:45713629-45713648 MS.gene66171:CDS 20.0%
! ATACCTGTTATTCTAATCTT+TGG + chr3.3:45713713-45713732 None:intergenic 25.0%
! ATATGTCCGTACTTTAAATT+CGG - chr3.3:45713551-45713570 MS.gene66171:CDS 25.0%
! CAGAAACATGAAATTTCTTA+CGG - chr3.3:45712801-45712820 MS.gene66171:CDS 25.0%
!!! ATTGATTTTCAAATTGGACA+AGG - chr3.3:45713635-45713654 MS.gene66171:CDS 25.0%
AATTGATGTTGTTCTTCCAA+GGG - chr3.3:45714105-45714124 MS.gene66171:CDS 30.0%
ACAAATTCAAGTGAATGTTG+CGG + chr3.3:45713071-45713090 None:intergenic 30.0%
ATGATATCGAGACAATTGAA+TGG - chr3.3:45713345-45713364 MS.gene66171:CDS 30.0%
ATTGTCTAGATTCTCAATTG+AGG + chr3.3:45712880-45712899 None:intergenic 30.0%
CATTCACTTGAATTTGTCAA+TGG - chr3.3:45713074-45713093 MS.gene66171:CDS 30.0%
CTTGATTGAAGAATTGATGA+AGG + chr3.3:45714290-45714309 None:intergenic 30.0%
TTCATAAAGTACATGCATGT+AGG + chr3.3:45713531-45713550 None:intergenic 30.0%
TTGTCTAGATTCTCAATTGA+GGG + chr3.3:45712879-45712898 None:intergenic 30.0%
! ATCTTATTGTTTCTTGTGCT+TGG - chr3.3:45714389-45714408 MS.gene66171:CDS 30.0%
AGAGAAGTGGAAGAAAAACA+TGG - chr3.3:45713259-45713278 MS.gene66171:CDS 35.0%
AGCAGATTCTTCTTGAACTT+TGG + chr3.3:45713222-45713241 None:intergenic 35.0%
ATCTGCTTCGTTCTTATTAG+AGG - chr3.3:45713235-45713254 MS.gene66171:CDS 35.0%
CAATTGATGTTGTTCTTCCA+AGG - chr3.3:45714104-45714123 MS.gene66171:CDS 35.0%
CCTCCAAAGATTAGAATAAC+AGG - chr3.3:45713707-45713726 MS.gene66171:CDS 35.0%
CCTGTTATTCTAATCTTTGG+AGG + chr3.3:45713710-45713729 None:intergenic 35.0%
CGATTTGTAGATTGTACTTC+CGG + chr3.3:45713471-45713490 None:intergenic 35.0%
GATGAGAAGGATAGAATGTT+TGG + chr3.3:45712926-45712945 None:intergenic 35.0%
TATCGAGACAATTGAATGGA+TGG - chr3.3:45713349-45713368 MS.gene66171:CDS 35.0%
TGGTACATGATGTAGCATAA+TGG + chr3.3:45713918-45713937 None:intergenic 35.0%
TTATACTTGTGCAAAAGCAG+TGG - chr3.3:45713316-45713335 MS.gene66171:CDS 35.0%
TTCGGATATATGTCTGCAAA+TGG - chr3.3:45713569-45713588 MS.gene66171:CDS 35.0%
!! TGTTCTAGAGTTTGATGAGA+AGG + chr3.3:45712939-45712958 None:intergenic 35.0%
AAATTGGACAAGGAACTCAG+TGG - chr3.3:45713645-45713664 MS.gene66171:CDS 40.0%
AACATGGAGATGGTATCTAG+AGG - chr3.3:45713275-45713294 MS.gene66171:CDS 40.0%
AAGGATACACTCTAACAGCA+TGG + chr3.3:45713835-45713854 None:intergenic 40.0%
AATTGGACAAGGAACTCAGT+GGG - chr3.3:45713646-45713665 MS.gene66171:CDS 40.0%
ACATGGAGATGGTATCTAGA+GGG - chr3.3:45713276-45713295 MS.gene66171:CDS 40.0%
ATAGTCCAGGTTCTACGTTA+AGG - chr3.3:45713045-45713064 MS.gene66171:CDS 40.0%
ATCATGACCATGATGACCTA+AGG - chr3.3:45713129-45713148 MS.gene66171:CDS 40.0%
ATCTCTAGCATCTCTACGTT+AGG + chr3.3:45713191-45713210 None:intergenic 40.0%
ATGAAGGAGCTCAATGGATA+AGG + chr3.3:45714274-45714293 None:intergenic 40.0%
ATTTGTCAATGGTTCACCTG+AGG - chr3.3:45713085-45713104 MS.gene66171:CDS 40.0%
CAAAATTCTCCCTCTACTCA+CGG - chr3.3:45713008-45713027 MS.gene66171:CDS 40.0%
CCATTCATAATCATCGTGAC+AGG - chr3.3:45713951-45713970 MS.gene66171:CDS 40.0%
CCTGTCACGATGATTATGAA+TGG + chr3.3:45713954-45713973 None:intergenic 40.0%
CGCAAAATAGTATCCGTTTC+AGG - chr3.3:45713383-45713402 MS.gene66171:CDS 40.0%
CTAACAGCATGGAACTCAAA+TGG + chr3.3:45713824-45713843 None:intergenic 40.0%
GAAAGCAGTTCATTACCAGT+AGG + chr3.3:45713509-45713528 None:intergenic 40.0%
GAATTGATGAAGGAGCTCAA+TGG + chr3.3:45714280-45714299 None:intergenic 40.0%
GTAGAACCTGGACTATTGTT+AGG + chr3.3:45713041-45713060 None:intergenic 40.0%
GTGGAAGAAAAACATGGAGA+TGG - chr3.3:45713265-45713284 MS.gene66171:CDS 40.0%
TAATGTCGAACAGCACTGTT+TGG - chr3.3:45714144-45714163 MS.gene66171:CDS 40.0%
TCTTATGCCTTAGGTCATCA+TGG + chr3.3:45713139-45713158 None:intergenic 40.0%
TCTTATTAGAGGCAGAGAAG+TGG - chr3.3:45713246-45713265 MS.gene66171:CDS 40.0%
TGAATGGTCACACTTTCATC+TGG + chr3.3:45713938-45713957 None:intergenic 40.0%
TGTTAGAGTGTATCCTTCTG+AGG - chr3.3:45713838-45713857 MS.gene66171:CDS 40.0%
TTCTCACCTAACAATAGTCC+AGG - chr3.3:45713032-45713051 MS.gene66171:CDS 40.0%
! AAATTTCTTACGGCTCTGAC+AGG - chr3.3:45712811-45712830 MS.gene66171:CDS 40.0%
! TGAACTTAGTAGCTTGCGAA+GGG - chr3.3:45714179-45714198 MS.gene66171:CDS 40.0%
! TGCTTGTTGAAGCAGATTCA+AGG + chr3.3:45712971-45712990 None:intergenic 40.0%
!! GTAGAGGGAGAATTTTGGTA+AGG + chr3.3:45713005-45713024 None:intergenic 40.0%
AAGGCATAAGATGAGTGAGC+TGG - chr3.3:45713148-45713167 MS.gene66171:CDS 45.0%
ACTTCGTCCTAATGAAGCTG+TGG - chr3.3:45713880-45713899 MS.gene66171:CDS 45.0%
ATCCTCTCTTTGTGATCCCT+TGG + chr3.3:45714124-45714143 None:intergenic 45.0%
CATGGAGATGGTATCTAGAG+GGG - chr3.3:45713277-45713296 MS.gene66171:CDS 45.0%
GAGCTGGAATCTGTTATGCT+CGG - chr3.3:45713164-45713183 MS.gene66171:CDS 45.0%
GCTCACTCATCTTATGCCTT+AGG + chr3.3:45713148-45713167 None:intergenic 45.0%
GTAGATTGTACTTCCGGAAG+AGG + chr3.3:45713465-45713484 None:intergenic 45.0%
GTCATGAGCTTCTGAGTAAG+TGG - chr3.3:45714218-45714237 MS.gene66171:CDS 45.0%
GTTTCGAAGATTGCCTCTTC+CGG - chr3.3:45713449-45713468 MS.gene66171:CDS 45.0%
TAAGCACATGTCTCCTAAGG+TGG - chr3.3:45713988-45714007 MS.gene66171:CDS 45.0%
TACGACTCGAGATTCTGTAC+AGG + chr3.3:45712849-45712868 None:intergenic 45.0%
TCAACTAGAGTCACCACCTT+AGG + chr3.3:45714004-45714023 None:intergenic 45.0%
TCTTATTCATCGTATGCCCG+CGG - chr3.3:45713740-45713759 MS.gene66171:CDS 45.0%
TGTTTGGCAGCTGTTATCAG+AGG + chr3.3:45712910-45712929 None:intergenic 45.0%
TTAAAATGCAGCGAGCCTAC+TGG - chr3.3:45713491-45713510 MS.gene66171:CDS 45.0%
TTCCAAGGGATCACAAAGAG+AGG - chr3.3:45714119-45714138 MS.gene66171:CDS 45.0%
TTCGATTGGACTGCCTGAAA+CGG + chr3.3:45713399-45713418 None:intergenic 45.0%
! AGAGATGGTGCACTGTTTCT+TGG - chr3.3:45714352-45714371 MS.gene66171:CDS 45.0%
! ATGGTGCACTGTTTCTTGGT+TGG - chr3.3:45714356-45714375 MS.gene66171:CDS 45.0%
! CGTGAGTAGAGGGAGAATTT+TGG + chr3.3:45713010-45713029 None:intergenic 45.0%
! GAACTTAGTAGCTTGCGAAG+GGG - chr3.3:45714180-45714199 MS.gene66171:CDS 45.0%
! GTGAACTTAGTAGCTTGCGA+AGG - chr3.3:45714178-45714197 MS.gene66171:CDS 45.0%
!!! AAAAAGCGTTTTGCGATGGC+TGG - chr3.3:45714241-45714260 MS.gene66171:CDS 45.0%
!!! GTGGAAAAAGCGTTTTGCGA+TGG - chr3.3:45714237-45714256 MS.gene66171:CDS 45.0%
AAGTAAGAATCCTCCGCCTC+AGG + chr3.3:45713104-45713123 None:intergenic 50.0%
AATCTTCTCGAGAGCTACCG+TGG - chr3.3:45714310-45714329 MS.gene66171:CDS 50.0%
AGGCAGTCCAATCGAACGAT+TGG - chr3.3:45713403-45713422 MS.gene66171:CDS 50.0%
AGGTGAGAAACCGTGAGTAG+AGG + chr3.3:45713021-45713040 None:intergenic 50.0%
CTGCTAGGGTGTAATGTCCA+CGG + chr3.3:45714330-45714349 None:intergenic 50.0%
GGCAGTCCAATCGAACGATT+GGG - chr3.3:45713404-45713423 MS.gene66171:CDS 50.0%
GGCTAAGCACATGTCTCCTA+AGG - chr3.3:45713985-45714004 MS.gene66171:CDS 50.0%
GGTGAGAAACCGTGAGTAGA+GGG + chr3.3:45713020-45713039 None:intergenic 50.0%
TACACCCTAGCAGAGAGAGA+TGG - chr3.3:45714337-45714356 MS.gene66171:CDS 50.0%
TACGCTCCCAATCGTTCGAT+TGG + chr3.3:45713413-45713432 None:intergenic 50.0%
TCGACGAAACGCTGCAAGAA+CGG + chr3.3:45714052-45714071 None:intergenic 50.0%
TCGTCCTAATGAAGCTGTGG+CGG - chr3.3:45713883-45713902 MS.gene66171:CDS 50.0%
TGCACCATCTCTCTCTGCTA+GGG + chr3.3:45714344-45714363 None:intergenic 50.0%
TGTCAATGGTTCACCTGAGG+CGG - chr3.3:45713088-45713107 MS.gene66171:CDS 50.0%
TTCACCGCCACAGCTTCATT+AGG + chr3.3:45713890-45713909 None:intergenic 50.0%
TTGCGAAGGGGAAGAAAGAG+TGG - chr3.3:45714192-45714211 MS.gene66171:CDS 50.0%
! ACGATTGGGAGCGTACATGT+TGG - chr3.3:45713418-45713437 MS.gene66171:CDS 50.0%
! ATTGGGAGCGTACATGTTGG+AGG - chr3.3:45713421-45713440 MS.gene66171:CDS 50.0%
! CTAATCTTTGGAGGTCCTCC+AGG + chr3.3:45713701-45713720 None:intergenic 50.0%
! CTGCAAGAACGGAGCGTTAT+TGG + chr3.3:45714041-45714060 None:intergenic 50.0%
!! GTGGGGGCGTTGATATAGTA+GGG - chr3.3:45713762-45713781 MS.gene66171:CDS 50.0%
!! TGGGGGCGTTGATATAGTAG+GGG - chr3.3:45713763-45713782 MS.gene66171:CDS 50.0%
AGCCATCGCGGAAGCTATGA+AGG - chr3.3:45713592-45713611 MS.gene66171:CDS 55.0%
ATTCATCGTATGCCCGCGGT+GGG - chr3.3:45713744-45713763 MS.gene66171:CDS 55.0%
CATCGCGGAAGCTATGAAGG+AGG - chr3.3:45713595-45713614 MS.gene66171:CDS 55.0%
CTCCTTCATAGCTTCCGCGA+TGG + chr3.3:45713597-45713616 None:intergenic 55.0%
GTCTGCAAATGGAGCCATCG+CGG - chr3.3:45713580-45713599 MS.gene66171:CDS 55.0%
GTGCACCATCTCTCTCTGCT+AGG + chr3.3:45714345-45714364 None:intergenic 55.0%
TAATCCAGGCTCTTGCTCGC+CGG - chr3.3:45713675-45713694 MS.gene66171:CDS 55.0%
TATTCATCGTATGCCCGCGG+TGG - chr3.3:45713743-45713762 MS.gene66171:CDS 55.0%
TCAGTGGGTGAGCCTAATCC+AGG - chr3.3:45713661-45713680 MS.gene66171:CDS 55.0%
TCGTGACAGGTTGCTGCGAT+TGG - chr3.3:45713964-45713983 MS.gene66171:CDS 55.0%
TCTTCGAGTCGCACCTCAGA+AGG + chr3.3:45713854-45713873 None:intergenic 55.0%
TGCGGCCTTAACGTAGAACC+TGG + chr3.3:45713053-45713072 None:intergenic 55.0%
!! GGTGGGGGCGTTGATATAGT+AGG - chr3.3:45713761-45713780 MS.gene66171:CDS 55.0%
CAATGGTTCACCTGAGGCGG+AGG - chr3.3:45713091-45713110 MS.gene66171:CDS 60.0%
CTATATCAACGCCCCCACCG+CGG + chr3.3:45713760-45713779 None:intergenic 60.0%
GGCGAGCAAGAGCCTGGATT+AGG + chr3.3:45713676-45713695 None:intergenic 60.0%
TATATCAACGCCCCCACCGC+GGG + chr3.3:45713759-45713778 None:intergenic 60.0%
TTCATCGTATGCCCGCGGTG+GGG - chr3.3:45713745-45713764 MS.gene66171:CDS 60.0%
! TCTTTGGAGGTCCTCCAGGC+CGG + chr3.3:45713697-45713716 None:intergenic 60.0%
TCATCGTATGCCCGCGGTGG+GGG - chr3.3:45713746-45713765 MS.gene66171:CDS 65.0%
CAGGCCGGCGAGCAAGAGCC+TGG + chr3.3:45713682-45713701 None:intergenic 75.0%
CAGGCTCTTGCTCGCCGGCC+TGG - chr3.3:45713680-45713699 MS.gene66171:CDS 75.0%
GCTCTTGCTCGCCGGCCTGG+AGG - chr3.3:45713683-45713702 MS.gene66171:CDS 75.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 45712789 45714417 45712789 ID=MS.gene66171
chr3.3 mRNA 45712789 45714417 45712789 ID=MS.gene66171.t1;Parent=MS.gene66171
chr3.3 exon 45712789 45714417 45712789 ID=MS.gene66171.t1.exon1;Parent=MS.gene66171.t1
chr3.3 CDS 45712789 45714417 45712789 ID=cds.MS.gene66171.t1;Parent=MS.gene66171.t1
Gene Sequence

>MS.gene66171

ATGCAAACATCTCAGAAACATGAAATTTCTTACGGCTCTGACAGGTTCTACGTTGAGCCTGTACAGAATCTCGAGTCGTATTGCTTACCCTCAATTGAGAATCTAGACAATTATTCTTCCTCTGATAACAGCTGCCAAACATTCTATCCTTCTCATCAAACTCTAGAACAATACAACAACCTTGAATCTGCTTCAACAAGCAACAACAGTTTACCTTACCAAAATTCTCCCTCTACTCACGGTTTCTCACCTAACAATAGTCCAGGTTCTACGTTAAGGCCGCAACATTCACTTGAATTTGTCAATGGTTCACCTGAGGCGGAGGATTCTTACTTGACATATCATGACCATGATGACCTAAGGCATAAGATGAGTGAGCTGGAATCTGTTATGCTCGGACCTAACGTAGAGATGCTAGAGATGTATGATACCAAAGTTCAAGAAGAATCTGCTTCGTTCTTATTAGAGGCAGAGAAGTGGAAGAAAAACATGGAGATGGTATCTAGAGGGGATTTGAAAGAGATGCTTTATACTTGTGCAAAAGCAGTGGATGTGAATGATATCGAGACAATTGAATGGATGGTAACTGAGTTACGCAAAATAGTATCCGTTTCAGGCAGTCCAATCGAACGATTGGGAGCGTACATGTTGGAGGCACTTGTTTCGAAGATTGCCTCTTCCGGAAGTACAATCTACAAATCGTTAAAATGCAGCGAGCCTACTGGTAATGAACTGCTTTCCTACATGCATGTACTTTATGAAATATGTCCGTACTTTAAATTCGGATATATGTCTGCAAATGGAGCCATCGCGGAAGCTATGAAGGAGGAAAATGAAGTCCACATAATTGATTTTCAAATTGGACAAGGAACTCAGTGGGTGAGCCTAATCCAGGCTCTTGCTCGCCGGCCTGGAGGACCTCCAAAGATTAGAATAACAGGTATTGATGACTCTTATTCATCGTATGCCCGCGGTGGGGGCGTTGATATAGTAGGGGAGAAGTTATTCGCGCTTGCGCAGTCATGTCATGTACCATTTGAGTTCCATGCTGTTAGAGTGTATCCTTCTGAGGTGCGACTCGAAGACTTTGAACTTCGTCCTAATGAAGCTGTGGCGGTGAACTTTGCCATTATGCTACATCATGTACCAGATGAAAGTGTGACCATTCATAATCATCGTGACAGGTTGCTGCGATTGGCTAAGCACATGTCTCCTAAGGTGGTGACTCTAGTTGAGCAAGAATTCAACACCAATAACGCTCCGTTCTTGCAGCGTTTCGTCGAGACAATGAACTACTACTCAGCCGTTTATGAATCAATTGATGTTGTTCTTCCAAGGGATCACAAAGAGAGGATTAATGTCGAACAGCACTGTTTGGCTCGTGAAGTTGTGAACTTAGTAGCTTGCGAAGGGGAAGAAAGAGTGGAACGTCATGAGCTTCTGAGTAAGTGGAAAAAGCGTTTTGCGATGGCTGGATTTACGCCTTATCCATTGAGCTCCTTCATCAATTCTTCAATCAAGAATCTTCTCGAGAGCTACCGTGGACATTACACCCTAGCAGAGAGAGATGGTGCACTGTTTCTTGGTTGGATGAATCAAGATCTTATTGTTTCTTGTGCTTGGAGATGA
Protein sequence

>MS.gene66171.t1

MQTSQKHEISYGSDRFYVEPVQNLESYCLPSIENLDNYSSSDNSCQTFYPSHQTLEQYNNLESASTSNNSLPYQNSPSTHGFSPNNSPGSTLRPQHSLEFVNGSPEAEDSYLTYHDHDDLRHKMSELESVMLGPNVEMLEMYDTKVQEESASFLLEAEKWKKNMEMVSRGDLKEMLYTCAKAVDVNDIETIEWMVTELRKIVSVSGSPIERLGAYMLEALVSKIASSGSTIYKSLKCSEPTGNELLSYMHVLYEICPYFKFGYMSANGAIAEAMKEENEVHIIDFQIGQGTQWVSLIQALARRPGGPPKIRITGIDDSYSSYARGGGVDIVGEKLFALAQSCHVPFEFHAVRVYPSEVRLEDFELRPNEAVAVNFAIMLHHVPDESVTIHNHRDRLLRLAKHMSPKVVTLVEQEFNTNNAPFLQRFVETMNYYSAVYESIDVVLPRDHKERINVEQHCLAREVVNLVACEGEERVERHELLSKWKKRFAMAGFTPYPLSSFINSSIKNLLESYRGHYTLAERDGALFLGWMNQDLIVSCAWR