Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66753.t1 | XP_013455825.1 | 97.2 | 362 | 10 | 0 | 1 | 362 | 1 | 362 | 5.40E-202 | 713.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66753.t1 | Q9ZQG9 | 63.2 | 356 | 127 | 2 | 6 | 360 | 9 | 361 | 2.0e-136 | 486.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66753.t1 | A0A072UKG4 | 97.2 | 362 | 10 | 0 | 1 | 362 | 1 | 362 | 3.9e-202 | 713.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049661 | MS.gene66753 | 0.806001 | 1.02E-49 | -1.69E-46 |
| MS.gene049662 | MS.gene66753 | 0.801009 | 1.10E-48 | -1.69E-46 |
| MS.gene052340 | MS.gene66753 | 0.83264 | 8.43E-56 | -1.69E-46 |
| MS.gene055116 | MS.gene66753 | 0.841209 | 5.46E-58 | -1.69E-46 |
| MS.gene056191 | MS.gene66753 | 0.811628 | 6.37E-51 | -1.69E-46 |
| MS.gene05702 | MS.gene66753 | 0.836772 | 7.69E-57 | -1.69E-46 |
| MS.gene057749 | MS.gene66753 | 0.825116 | 5.58E-54 | -1.69E-46 |
| MS.gene057823 | MS.gene66753 | 0.811646 | 6.31E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene66753 | 0.801243 | 9.87E-49 | -1.69E-46 |
| MS.gene058710 | MS.gene66753 | 0.800023 | 1.75E-48 | -1.69E-46 |
| MS.gene059285 | MS.gene66753 | 0.816941 | 4.26E-52 | -1.69E-46 |
| MS.gene06037 | MS.gene66753 | 0.824724 | 6.91E-54 | -1.69E-46 |
| MS.gene061049 | MS.gene66753 | 0.837816 | 4.16E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene064895 | MS.gene66753 | PPI |
| MS.gene66753 | MS.gene45685 | PPI |
| MS.gene66753 | MS.gene003763 | PPI |
| MS.gene063164 | MS.gene66753 | PPI |
| MS.gene66753 | MS.gene68221 | PPI |
| MS.gene66753 | MS.gene014280 | PPI |
| MS.gene66753 | MS.gene50078 | PPI |
| MS.gene66753 | MS.gene056701 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66753.t1 | MTR_4g052970 | 97.238 | 362 | 10 | 0 | 1 | 362 | 1 | 362 | 0.0 | 722 |
| MS.gene66753.t1 | MTR_2g086530 | 65.489 | 368 | 119 | 2 | 1 | 362 | 1 | 366 | 6.33e-176 | 494 |
| MS.gene66753.t1 | MTR_2g086530 | 67.638 | 343 | 103 | 2 | 26 | 362 | 5 | 345 | 3.37e-171 | 481 |
| MS.gene66753.t1 | MTR_1g077990 | 59.062 | 320 | 130 | 1 | 28 | 347 | 30 | 348 | 1.56e-140 | 405 |
| MS.gene66753.t1 | MTR_4g084480 | 51.320 | 341 | 164 | 1 | 10 | 350 | 15 | 353 | 2.35e-128 | 374 |
| MS.gene66753.t1 | MTR_4g084480 | 51.320 | 341 | 164 | 1 | 10 | 350 | 8 | 346 | 2.43e-128 | 373 |
| MS.gene66753.t1 | MTR_4g084480 | 51.320 | 341 | 164 | 1 | 10 | 350 | 15 | 353 | 2.94e-128 | 374 |
| MS.gene66753.t1 | MTR_2g019345 | 49.563 | 343 | 170 | 2 | 5 | 347 | 2 | 341 | 6.43e-123 | 360 |
| MS.gene66753.t1 | MTR_4g010200 | 43.820 | 356 | 194 | 2 | 9 | 358 | 8 | 363 | 1.98e-110 | 328 |
| MS.gene66753.t1 | MTR_4g114850 | 47.368 | 323 | 169 | 1 | 28 | 349 | 25 | 347 | 2.60e-109 | 324 |
| MS.gene66753.t1 | MTR_4g114850 | 47.368 | 323 | 169 | 1 | 28 | 349 | 25 | 347 | 3.52e-109 | 324 |
| MS.gene66753.t1 | MTR_4g114850 | 47.368 | 323 | 169 | 1 | 28 | 349 | 25 | 347 | 3.99e-109 | 324 |
| MS.gene66753.t1 | MTR_3g080410 | 46.866 | 335 | 166 | 5 | 25 | 350 | 22 | 353 | 5.88e-99 | 298 |
| MS.gene66753.t1 | MTR_4g069940 | 44.575 | 341 | 187 | 2 | 9 | 347 | 8 | 348 | 1.18e-97 | 298 |
| MS.gene66753.t1 | MTR_1g056640 | 47.059 | 323 | 166 | 4 | 28 | 347 | 37 | 357 | 7.35e-96 | 294 |
| MS.gene66753.t1 | MTR_1g007810 | 44.311 | 334 | 175 | 5 | 13 | 344 | 5 | 329 | 4.25e-95 | 288 |
| MS.gene66753.t1 | MTR_3g116510 | 42.486 | 346 | 193 | 2 | 3 | 347 | 7 | 347 | 5.98e-95 | 290 |
| MS.gene66753.t1 | MTR_5g085580 | 46.483 | 327 | 168 | 4 | 25 | 347 | 21 | 344 | 7.14e-95 | 291 |
| MS.gene66753.t1 | MTR_1g022160 | 43.925 | 321 | 178 | 2 | 29 | 347 | 24 | 344 | 9.88e-95 | 288 |
| MS.gene66753.t1 | MTR_1g022160 | 43.925 | 321 | 178 | 2 | 29 | 347 | 24 | 344 | 5.80e-94 | 289 |
| MS.gene66753.t1 | MTR_1g022160 | 43.925 | 321 | 178 | 2 | 29 | 347 | 24 | 344 | 7.41e-94 | 288 |
| MS.gene66753.t1 | MTR_1g022160 | 43.925 | 321 | 178 | 2 | 29 | 347 | 24 | 344 | 9.83e-94 | 288 |
| MS.gene66753.t1 | MTR_6g088885 | 43.425 | 327 | 177 | 3 | 30 | 352 | 28 | 350 | 1.27e-89 | 278 |
| MS.gene66753.t1 | MTR_7g053500 | 39.521 | 334 | 196 | 5 | 30 | 358 | 27 | 359 | 1.42e-86 | 269 |
| MS.gene66753.t1 | MTR_7g026340 | 41.925 | 322 | 179 | 6 | 28 | 347 | 22 | 337 | 2.94e-83 | 260 |
| MS.gene66753.t1 | MTR_8g102340 | 41.618 | 346 | 166 | 5 | 4 | 349 | 5 | 314 | 5.66e-81 | 255 |
| MS.gene66753.t1 | MTR_4g088155 | 37.572 | 346 | 205 | 6 | 11 | 349 | 8 | 349 | 4.53e-79 | 250 |
| MS.gene66753.t1 | MTR_8g012400 | 37.681 | 345 | 206 | 7 | 14 | 353 | 17 | 357 | 6.63e-79 | 249 |
| MS.gene66753.t1 | MTR_1g031900 | 38.081 | 344 | 206 | 4 | 10 | 349 | 7 | 347 | 2.88e-78 | 249 |
| MS.gene66753.t1 | MTR_1g031900 | 38.081 | 344 | 206 | 4 | 10 | 349 | 7 | 347 | 2.97e-78 | 249 |
| MS.gene66753.t1 | MTR_4g083500 | 41.875 | 320 | 181 | 5 | 30 | 347 | 45 | 361 | 2.79e-76 | 244 |
| MS.gene66753.t1 | MTR_8g085720 | 36.364 | 352 | 219 | 3 | 8 | 355 | 5 | 355 | 7.83e-75 | 240 |
| MS.gene66753.t1 | MTR_4g076490 | 37.574 | 338 | 195 | 6 | 15 | 349 | 81 | 405 | 5.53e-73 | 233 |
| MS.gene66753.t1 | MTR_3g065460 | 37.615 | 327 | 195 | 4 | 29 | 349 | 24 | 347 | 1.31e-72 | 234 |
| MS.gene66753.t1 | MTR_4g132280 | 37.349 | 332 | 203 | 3 | 22 | 349 | 38 | 368 | 3.64e-72 | 233 |
| MS.gene66753.t1 | MTR_4g076470 | 37.313 | 335 | 193 | 7 | 10 | 343 | 6 | 324 | 2.24e-71 | 226 |
| MS.gene66753.t1 | MTR_4g076440 | 38.166 | 338 | 195 | 7 | 9 | 345 | 2 | 326 | 1.00e-70 | 224 |
| MS.gene66753.t1 | MTR_1g052430 | 39.169 | 337 | 187 | 9 | 27 | 360 | 30 | 351 | 3.86e-70 | 225 |
| MS.gene66753.t1 | MTR_4g076255 | 38.365 | 318 | 183 | 5 | 28 | 345 | 24 | 328 | 7.64e-70 | 222 |
| MS.gene66753.t1 | MTR_7g053500 | 39.286 | 280 | 164 | 5 | 84 | 358 | 4 | 282 | 8.70e-70 | 223 |
| MS.gene66753.t1 | MTR_3g083580 | 34.718 | 337 | 213 | 5 | 12 | 343 | 10 | 344 | 3.97e-69 | 225 |
| MS.gene66753.t1 | MTR_4g076570 | 39.062 | 320 | 187 | 5 | 28 | 346 | 21 | 333 | 2.16e-68 | 219 |
| MS.gene66753.t1 | MTR_5g078200 | 34.770 | 348 | 217 | 5 | 9 | 349 | 2 | 346 | 8.02e-68 | 225 |
| MS.gene66753.t1 | MTR_4g076430 | 36.982 | 338 | 194 | 9 | 10 | 345 | 6 | 326 | 1.04e-66 | 214 |
| MS.gene66753.t1 | MTR_2g034470 | 37.879 | 330 | 193 | 8 | 23 | 349 | 24 | 344 | 5.59e-65 | 210 |
| MS.gene66753.t1 | MTR_5g078200 | 35.759 | 316 | 194 | 4 | 40 | 349 | 1 | 313 | 9.96e-65 | 216 |
| MS.gene66753.t1 | MTR_2g034440 | 37.695 | 321 | 188 | 8 | 23 | 340 | 16 | 327 | 1.79e-63 | 206 |
| MS.gene66753.t1 | MTR_4g076500 | 36.337 | 344 | 203 | 6 | 3 | 339 | 46 | 380 | 1.94e-63 | 207 |
| MS.gene66753.t1 | MTR_3g116510 | 46.009 | 213 | 115 | 0 | 135 | 347 | 1 | 213 | 1.08e-62 | 203 |
| MS.gene66753.t1 | MTR_2g034470 | 38.486 | 317 | 183 | 8 | 36 | 349 | 2 | 309 | 1.72e-62 | 203 |
| MS.gene66753.t1 | MTR_7g081350 | 33.861 | 316 | 199 | 6 | 29 | 343 | 27 | 333 | 1.79e-62 | 203 |
| MS.gene66753.t1 | MTR_8g012400 | 38.182 | 275 | 164 | 5 | 84 | 353 | 4 | 277 | 2.94e-62 | 204 |
| MS.gene66753.t1 | MTR_2g034480 | 35.072 | 345 | 214 | 7 | 7 | 349 | 5 | 341 | 4.00e-62 | 203 |
| MS.gene66753.t1 | MTR_7g081370 | 31.953 | 338 | 207 | 7 | 7 | 343 | 6 | 321 | 5.30e-56 | 186 |
| MS.gene66753.t1 | MTR_8g092070 | 32.927 | 328 | 205 | 10 | 30 | 349 | 35 | 355 | 7.35e-54 | 187 |
| MS.gene66753.t1 | MTR_1g010120 | 32.055 | 365 | 218 | 13 | 7 | 361 | 6 | 350 | 7.92e-51 | 176 |
| MS.gene66753.t1 | MTR_2g027560 | 33.435 | 329 | 204 | 10 | 28 | 347 | 27 | 349 | 2.42e-47 | 167 |
| MS.gene66753.t1 | MTR_5g081720 | 33.951 | 324 | 201 | 9 | 26 | 344 | 34 | 349 | 2.61e-46 | 162 |
| MS.gene66753.t1 | MTR_6g032820 | 32.011 | 353 | 214 | 13 | 5 | 343 | 4 | 344 | 5.74e-46 | 162 |
| MS.gene66753.t1 | MTR_4g124440 | 30.423 | 355 | 225 | 14 | 6 | 349 | 6 | 349 | 1.09e-45 | 163 |
| MS.gene66753.t1 | MTR_7g085390 | 32.143 | 336 | 206 | 13 | 28 | 351 | 25 | 350 | 5.08e-45 | 161 |
| MS.gene66753.t1 | MTR_3g108600 | 31.232 | 349 | 197 | 11 | 6 | 347 | 19 | 331 | 1.91e-44 | 159 |
| MS.gene66753.t1 | MTR_4g112025 | 31.956 | 363 | 220 | 14 | 2 | 350 | 6 | 355 | 3.27e-44 | 159 |
| MS.gene66753.t1 | MTR_1g053320 | 30.571 | 350 | 222 | 11 | 10 | 349 | 16 | 354 | 3.75e-44 | 159 |
| MS.gene66753.t1 | MTR_3g095050 | 30.882 | 340 | 210 | 15 | 28 | 353 | 33 | 361 | 4.83e-43 | 156 |
| MS.gene66753.t1 | MTR_3g031770 | 30.514 | 331 | 212 | 11 | 28 | 349 | 26 | 347 | 3.61e-38 | 142 |
| MS.gene66753.t1 | MTR_4g134280 | 34.340 | 265 | 148 | 11 | 116 | 361 | 3 | 260 | 6.32e-31 | 121 |
| MS.gene66753.t1 | MTR_6g078180 | 76.000 | 50 | 12 | 0 | 92 | 141 | 5 | 54 | 1.76e-20 | 84.3 |
| MS.gene66753.t1 | MTR_4g036775 | 38.235 | 102 | 56 | 4 | 245 | 345 | 80 | 175 | 6.20e-15 | 73.6 |
| MS.gene66753.t1 | MTR_4g036775 | 37.349 | 83 | 52 | 0 | 10 | 92 | 3 | 85 | 8.32e-11 | 61.6 |
| MS.gene66753.t1 | MTR_4g036680 | 38.235 | 102 | 56 | 4 | 245 | 345 | 80 | 175 | 6.70e-15 | 73.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66753.t1 | AT2G27500 | 64.118 | 340 | 121 | 1 | 22 | 360 | 22 | 361 | 1.88e-172 | 486 |
| MS.gene66753.t1 | AT2G27500 | 64.118 | 340 | 121 | 1 | 22 | 360 | 22 | 361 | 2.57e-172 | 484 |
| MS.gene66753.t1 | AT2G27500 | 63.251 | 283 | 103 | 1 | 79 | 360 | 1 | 283 | 1.06e-141 | 404 |
| MS.gene66753.t1 | AT1G32860 | 53.868 | 349 | 159 | 1 | 1 | 347 | 1 | 349 | 2.89e-139 | 402 |
| MS.gene66753.t1 | AT5G42100 | 53.704 | 324 | 147 | 2 | 28 | 348 | 26 | 349 | 5.98e-125 | 366 |
| MS.gene66753.t1 | AT5G42100 | 53.704 | 324 | 147 | 2 | 28 | 348 | 26 | 349 | 6.67e-125 | 366 |
| MS.gene66753.t1 | AT4G18340 | 48.276 | 348 | 177 | 2 | 3 | 347 | 5 | 352 | 4.58e-122 | 358 |
| MS.gene66753.t1 | AT4G18340 | 48.276 | 348 | 177 | 2 | 3 | 347 | 5 | 352 | 5.49e-122 | 357 |
| MS.gene66753.t1 | AT1G30080 | 48.235 | 340 | 171 | 2 | 10 | 346 | 16 | 353 | 1.31e-116 | 343 |
| MS.gene66753.t1 | AT1G30080 | 48.235 | 340 | 171 | 2 | 10 | 346 | 16 | 353 | 1.82e-116 | 344 |
| MS.gene66753.t1 | AT3G15800 | 49.383 | 324 | 162 | 2 | 28 | 349 | 42 | 365 | 1.89e-115 | 341 |
| MS.gene66753.t1 | AT2G26600 | 44.985 | 329 | 177 | 2 | 23 | 347 | 25 | 353 | 1.14e-109 | 326 |
| MS.gene66753.t1 | AT4G26830 | 46.131 | 336 | 176 | 4 | 12 | 345 | 9 | 341 | 1.34e-103 | 313 |
| MS.gene66753.t1 | AT4G26830 | 47.799 | 318 | 162 | 3 | 30 | 345 | 30 | 345 | 3.71e-103 | 311 |
| MS.gene66753.t1 | AT5G55180 | 45.201 | 323 | 174 | 3 | 29 | 349 | 27 | 348 | 3.50e-97 | 296 |
| MS.gene66753.t1 | AT5G55180 | 45.201 | 323 | 174 | 3 | 29 | 349 | 27 | 348 | 3.67e-97 | 296 |
| MS.gene66753.t1 | AT2G05790 | 46.894 | 322 | 164 | 4 | 28 | 345 | 24 | 342 | 1.26e-95 | 293 |
| MS.gene66753.t1 | AT2G16230 | 45.231 | 325 | 176 | 2 | 25 | 347 | 20 | 344 | 4.40e-94 | 290 |
| MS.gene66753.t1 | AT4G34480 | 44.248 | 339 | 187 | 2 | 11 | 347 | 7 | 345 | 6.75e-94 | 285 |
| MS.gene66753.t1 | AT4G34480 | 43.429 | 350 | 196 | 2 | 11 | 358 | 7 | 356 | 5.69e-92 | 284 |
| MS.gene66753.t1 | AT5G24318 | 42.857 | 322 | 178 | 3 | 28 | 347 | 29 | 346 | 1.81e-91 | 279 |
| MS.gene66753.t1 | AT2G26600 | 47.082 | 257 | 136 | 0 | 91 | 347 | 3 | 259 | 7.69e-91 | 275 |
| MS.gene66753.t1 | AT5G42720 | 43.567 | 342 | 184 | 4 | 10 | 347 | 10 | 346 | 8.36e-91 | 279 |
| MS.gene66753.t1 | AT5G24318 | 40.988 | 344 | 197 | 3 | 6 | 347 | 7 | 346 | 2.47e-90 | 278 |
| MS.gene66753.t1 | AT5G24318 | 40.988 | 344 | 197 | 3 | 6 | 347 | 7 | 346 | 4.14e-90 | 278 |
| MS.gene66753.t1 | AT5G56590 | 41.411 | 326 | 189 | 2 | 26 | 349 | 23 | 348 | 3.45e-87 | 272 |
| MS.gene66753.t1 | AT4G29360 | 42.939 | 347 | 189 | 4 | 4 | 349 | 9 | 347 | 4.65e-84 | 265 |
| MS.gene66753.t1 | AT4G29360 | 44.237 | 321 | 177 | 2 | 30 | 349 | 38 | 357 | 9.18e-84 | 264 |
| MS.gene66753.t1 | AT3G07320 | 41.231 | 325 | 185 | 5 | 30 | 349 | 26 | 349 | 4.62e-83 | 260 |
| MS.gene66753.t1 | AT2G01630 | 39.385 | 325 | 192 | 3 | 29 | 349 | 23 | 346 | 3.08e-79 | 248 |
| MS.gene66753.t1 | AT2G01630 | 39.385 | 325 | 192 | 3 | 29 | 349 | 23 | 346 | 1.05e-78 | 248 |
| MS.gene66753.t1 | AT2G01630 | 39.385 | 325 | 192 | 3 | 29 | 349 | 23 | 346 | 7.82e-78 | 248 |
| MS.gene66753.t1 | AT4G16260 | 38.690 | 336 | 198 | 4 | 10 | 345 | 5 | 332 | 4.51e-77 | 241 |
| MS.gene66753.t1 | AT4G14080 | 38.226 | 327 | 197 | 4 | 30 | 352 | 43 | 368 | 7.31e-76 | 242 |
| MS.gene66753.t1 | AT2G39640 | 39.118 | 340 | 197 | 5 | 10 | 347 | 9 | 340 | 1.73e-75 | 243 |
| MS.gene66753.t1 | AT3G23770 | 38.838 | 327 | 192 | 5 | 30 | 349 | 38 | 363 | 1.93e-74 | 238 |
| MS.gene66753.t1 | AT4G16260 | 39.355 | 310 | 180 | 4 | 36 | 345 | 2 | 303 | 1.00e-72 | 229 |
| MS.gene66753.t1 | AT3G55430 | 38.372 | 344 | 192 | 11 | 28 | 360 | 28 | 362 | 2.28e-72 | 232 |
| MS.gene66753.t1 | AT3G13560 | 35.913 | 323 | 202 | 3 | 25 | 343 | 22 | 343 | 4.02e-72 | 233 |
| MS.gene66753.t1 | AT3G13560 | 35.913 | 323 | 202 | 3 | 25 | 343 | 22 | 343 | 4.02e-72 | 233 |
| MS.gene66753.t1 | AT3G13560 | 35.913 | 323 | 202 | 3 | 25 | 343 | 22 | 343 | 4.02e-72 | 233 |
| MS.gene66753.t1 | AT3G13560 | 35.913 | 323 | 202 | 3 | 25 | 343 | 22 | 343 | 4.02e-72 | 233 |
| MS.gene66753.t1 | AT3G13560 | 35.913 | 323 | 202 | 3 | 25 | 343 | 22 | 343 | 4.02e-72 | 233 |
| MS.gene66753.t1 | AT1G11820 | 37.346 | 324 | 198 | 3 | 30 | 349 | 45 | 367 | 2.27e-71 | 228 |
| MS.gene66753.t1 | AT1G11820 | 37.346 | 324 | 198 | 3 | 30 | 349 | 45 | 367 | 2.33e-70 | 228 |
| MS.gene66753.t1 | AT1G11820 | 37.346 | 324 | 198 | 3 | 30 | 349 | 45 | 367 | 7.90e-70 | 227 |
| MS.gene66753.t1 | AT3G57270 | 37.152 | 323 | 191 | 7 | 26 | 345 | 24 | 337 | 8.94e-70 | 222 |
| MS.gene66753.t1 | AT4G34480 | 47.325 | 243 | 128 | 0 | 116 | 358 | 3 | 245 | 3.10e-67 | 217 |
| MS.gene66753.t1 | AT1G66250 | 35.583 | 326 | 204 | 4 | 26 | 347 | 29 | 352 | 1.03e-66 | 219 |
| MS.gene66753.t1 | AT3G46570 | 37.791 | 344 | 201 | 9 | 10 | 348 | 10 | 345 | 2.63e-66 | 214 |
| MS.gene66753.t1 | AT3G61810 | 34.365 | 323 | 196 | 5 | 30 | 345 | 56 | 369 | 5.80e-62 | 203 |
| MS.gene66753.t1 | AT5G24318 | 45.024 | 211 | 111 | 2 | 138 | 347 | 1 | 207 | 1.27e-58 | 192 |
| MS.gene66753.t1 | AT3G57260 | 32.938 | 337 | 211 | 8 | 10 | 345 | 14 | 336 | 4.03e-58 | 192 |
| MS.gene66753.t1 | AT3G57260 | 32.938 | 337 | 211 | 8 | 10 | 345 | 30 | 352 | 7.48e-58 | 192 |
| MS.gene66753.t1 | AT1G77780 | 34.018 | 341 | 214 | 8 | 6 | 345 | 2 | 332 | 5.24e-57 | 190 |
| MS.gene66753.t1 | AT1G77790 | 35.423 | 319 | 195 | 7 | 30 | 345 | 31 | 341 | 5.31e-57 | 189 |
| MS.gene66753.t1 | AT5G20340 | 36.137 | 321 | 193 | 9 | 28 | 345 | 42 | 353 | 5.70e-56 | 187 |
| MS.gene66753.t1 | AT3G57240 | 33.968 | 315 | 193 | 8 | 30 | 343 | 36 | 336 | 4.64e-54 | 181 |
| MS.gene66753.t1 | AT5G20390 | 34.226 | 336 | 209 | 8 | 13 | 345 | 17 | 343 | 1.06e-53 | 181 |
| MS.gene66753.t1 | AT5G20330 | 33.913 | 345 | 216 | 9 | 4 | 345 | 9 | 344 | 3.69e-52 | 177 |
| MS.gene66753.t1 | AT3G55780 | 36.304 | 303 | 177 | 9 | 42 | 336 | 48 | 342 | 2.28e-49 | 170 |
| MS.gene66753.t1 | AT1G64760 | 32.948 | 346 | 213 | 12 | 13 | 349 | 11 | 346 | 5.55e-49 | 172 |
| MS.gene66753.t1 | AT1G64760 | 32.948 | 346 | 213 | 12 | 13 | 349 | 11 | 346 | 5.55e-49 | 172 |
| MS.gene66753.t1 | AT2G19440 | 33.433 | 335 | 204 | 12 | 24 | 349 | 18 | 342 | 2.40e-48 | 170 |
| MS.gene66753.t1 | AT2G19440 | 33.433 | 335 | 204 | 12 | 24 | 349 | 18 | 342 | 2.40e-48 | 170 |
| MS.gene66753.t1 | AT1G33220 | 32.571 | 350 | 210 | 10 | 4 | 345 | 3 | 334 | 4.93e-48 | 166 |
| MS.gene66753.t1 | AT5G20870 | 32.982 | 379 | 221 | 16 | 1 | 359 | 1 | 366 | 6.68e-48 | 169 |
| MS.gene66753.t1 | AT5G18220 | 29.326 | 341 | 228 | 9 | 28 | 362 | 29 | 362 | 9.70e-46 | 163 |
| MS.gene66753.t1 | AT5G58090 | 31.707 | 328 | 209 | 10 | 28 | 347 | 21 | 341 | 5.48e-44 | 158 |
| MS.gene66753.t1 | AT4G31140 | 29.855 | 345 | 227 | 10 | 11 | 347 | 9 | 346 | 3.77e-43 | 156 |
| MS.gene66753.t1 | AT3G04010 | 30.411 | 365 | 233 | 10 | 9 | 362 | 13 | 367 | 7.50e-43 | 155 |
| MS.gene66753.t1 | AT3G24330 | 32.194 | 351 | 219 | 12 | 10 | 349 | 20 | 362 | 9.74e-42 | 152 |
| MS.gene66753.t1 | AT5G64790 | 28.571 | 336 | 225 | 10 | 28 | 355 | 27 | 355 | 1.51e-41 | 152 |
| MS.gene66753.t1 | AT5G58480 | 31.288 | 326 | 209 | 9 | 30 | 347 | 27 | 345 | 5.85e-41 | 150 |
| MS.gene66753.t1 | AT5G20560 | 30.240 | 334 | 219 | 8 | 10 | 337 | 11 | 336 | 2.31e-38 | 140 |
| MS.gene66753.t1 | AT4G17180 | 29.179 | 329 | 211 | 11 | 28 | 347 | 24 | 339 | 3.78e-36 | 137 |
Find 58 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTTCTTCTCACCATTTC+AGG | 0.103870 | 4.3:-46937040 | MS.gene66753:intron |
| AACAGTTATGCAATTTATTT+TGG | 0.148253 | 4.3:+46936651 | None:intergenic |
| TCAAATGTTGAATTTGTTAT+AGG | 0.179247 | 4.3:-46936763 | MS.gene66753:CDS |
| ACCCTTCTAAAGCTCAAAAT+TGG | 0.203490 | 4.3:-46936708 | MS.gene66753:CDS |
| CAAATCAATGGCTTAAGTTT+TGG | 0.205246 | 4.3:-46936916 | MS.gene66753:CDS |
| GTTGAATTTGTTATAGGAAT+TGG | 0.226002 | 4.3:-46936757 | MS.gene66753:CDS |
| AACAAATTCAACATTTGATT+TGG | 0.238369 | 4.3:+46936768 | None:intergenic |
| TAGTTTAAAGATACCTCATT+AGG | 0.279200 | 4.3:+46936349 | None:intergenic |
| GAGAAAGCAAGTAGAACATT+TGG | 0.321710 | 4.3:+46936790 | None:intergenic |
| AATGGCGACACCGATGAAAT+TGG | 0.322115 | 4.3:-46936169 | MS.gene66753:CDS |
| AGTTTATAACATTGGATTAC+AGG | 0.325008 | 4.3:-46935960 | MS.gene66753:CDS |
| TCATAATGCAAATTTGTGTT+TGG | 0.354916 | 4.3:+46936292 | None:intergenic |
| TCATCAAGAAATTCAACAAT+TGG | 0.359860 | 4.3:+46936427 | None:intergenic |
| ATTGGAACCAATGGATTTCC+AGG | 0.366015 | 4.3:+46936076 | None:intergenic |
| TTATACCAATGTTGAAGTTA+AGG | 0.367370 | 4.3:-46936215 | MS.gene66753:CDS |
| GGATCAACTAAACCTTCATT+TGG | 0.377257 | 4.3:+46936313 | None:intergenic |
| TGTAATCCAATGTTATAAAC+TGG | 0.399547 | 4.3:+46935962 | None:intergenic |
| CCTGCTTCAGAAAGAAACTA+TGG | 0.401079 | 4.3:-46936007 | MS.gene66753:CDS |
| AGTTTAAAGATACCTCATTA+GGG | 0.402444 | 4.3:+46936350 | None:intergenic |
| TATGCTGCAATCAAAGTGAT+AGG | 0.404460 | 4.3:-46936238 | MS.gene66753:CDS |
| CATAATTCTCTTGTTAAGCT+TGG | 0.406983 | 4.3:-46936559 | MS.gene66753:CDS |
| AAATAATAAGGAACCTGAAA+TGG | 0.423025 | 4.3:+46937027 | None:intergenic |
| CCATAGTTTCTTTCTGAAGC+AGG | 0.437326 | 4.3:+46936007 | None:intergenic |
| GAAGTTAAGGTTTCTGAGAC+AGG | 0.447516 | 4.3:-46936202 | MS.gene66753:CDS |
| CTGTTGTAACAGTAATTACT+TGG | 0.448483 | 4.3:+46936530 | None:intergenic |
| AAGTGATTTGAAGAAGGAAA+AGG | 0.449912 | 4.3:+46937066 | None:intergenic |
| GATTCATTTAAGCAAATCAA+TGG | 0.450746 | 4.3:-46936928 | MS.gene66753:CDS |
| AATTCTTACCCTCCTTCATC+CGG | 0.452830 | 4.3:-46936484 | MS.gene66753:CDS |
| GAGGAAAGACATGGAACTCC+TGG | 0.456953 | 4.3:-46936094 | MS.gene66753:CDS |
| CAAATCTGACCTAAAAGAAC+CGG | 0.460446 | 4.3:+46936465 | None:intergenic |
| AAAATAAATTGCATAACTGT+TGG | 0.468767 | 4.3:-46936649 | MS.gene66753:CDS |
| ACAGGGTTATCTACCACAAA+TGG | 0.472431 | 4.3:-46935942 | MS.gene66753:CDS |
| GAGACAGGTTGGCCTTCAAA+TGG | 0.474442 | 4.3:-46936187 | MS.gene66753:CDS |
| GCATTATTTCAACCAAATGA+AGG | 0.475383 | 4.3:-46936325 | MS.gene66753:CDS |
| GGTAGACCAGTTTATAACAT+TGG | 0.488178 | 4.3:-46935968 | MS.gene66753:CDS |
| GAAAAGAAGTGATTTGAAGA+AGG | 0.488719 | 4.3:+46937060 | None:intergenic |
| TTTCTTTCTGAAGCAGGACC+AGG | 0.491860 | 4.3:+46936013 | None:intergenic |
| AAATTTGCTTAGAAGAATTG+AGG | 0.496626 | 4.3:-46936113 | MS.gene66753:CDS |
| TTATAAACTGGTCTACCATC+AGG | 0.497918 | 4.3:+46935974 | None:intergenic |
| TTGGATGGCACAACCATTTG+TGG | 0.503269 | 4.3:+46935929 | None:intergenic |
| TCATCGGTGTCGCCATTTGA+AGG | 0.506564 | 4.3:+46936175 | None:intergenic |
| TATACATCAATTGGAACCAA+TGG | 0.507282 | 4.3:+46936067 | None:intergenic |
| TGGTGTAGCTCCAATTTCAT+CGG | 0.509224 | 4.3:+46936159 | None:intergenic |
| AGAAACCTTAACTTCAACAT+TGG | 0.523456 | 4.3:+46936210 | None:intergenic |
| TTGCTTACATTAGATTTGGA+TGG | 0.524160 | 4.3:+46935914 | None:intergenic |
| TCTTGAATGAGATGGTAGAT+TGG | 0.531449 | 4.3:+46936870 | None:intergenic |
| TATGGACTTTATTATCCTGA+TGG | 0.553442 | 4.3:-46935989 | MS.gene66753:CDS |
| TTTAATGAAGATTTGAAACC+TGG | 0.554891 | 4.3:-46936031 | MS.gene66753:CDS |
| TGGAACTCCTGGAAATCCAT+TGG | 0.557029 | 4.3:-46936083 | MS.gene66753:CDS |
| GACCTAAAAGAACCGGATGA+AGG | 0.558870 | 4.3:+46936472 | None:intergenic |
| TTAAGGTTTCTGAGACAGGT+TGG | 0.560373 | 4.3:-46936198 | MS.gene66753:CDS |
| GTTTATAACATTGGATTACA+GGG | 0.597138 | 4.3:-46935959 | MS.gene66753:CDS |
| ACTGCAACTCTTGAATGAGA+TGG | 0.599503 | 4.3:+46936862 | None:intergenic |
| CTAAAAGAACCGGATGAAGG+AGG | 0.608879 | 4.3:+46936475 | None:intergenic |
| TAAAAGAACCGGATGAAGGA+GGG | 0.617096 | 4.3:+46936476 | None:intergenic |
| ATATAAAGATAACCCTAATG+AGG | 0.620782 | 4.3:-46936362 | MS.gene66753:CDS |
| AGAAGAATTGAGGAAAGACA+TGG | 0.624353 | 4.3:-46936103 | MS.gene66753:CDS |
| CAAAATGCAAAATTGTACAA+TGG | 0.632067 | 4.3:-46936136 | MS.gene66753:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACATAAAAATTAATCTCAAA+AGG | + | chr4.3:46936985-46937004 | None:intergenic | 15.0% |
| !! | TATCTTTATATGCAAAAAAA+GGG | + | chr4.3:46936491-46936510 | None:intergenic | 15.0% |
| !! | TTATCTTTATATGCAAAAAA+AGG | + | chr4.3:46936492-46936511 | None:intergenic | 15.0% |
| !! | AAAATAAATTGCATAACTGT+TGG | - | chr4.3:46936213-46936232 | MS.gene66753:CDS | 20.0% |
| !! | AACAAATTCAACATTTGATT+TGG | + | chr4.3:46936097-46936116 | None:intergenic | 20.0% |
| !! | TAGAGAGAAGATAAATAATA+AGG | + | chr4.3:46935850-46935869 | None:intergenic | 20.0% |
| !!! | AACAGTTATGCAATTTATTT+TGG | + | chr4.3:46936214-46936233 | None:intergenic | 20.0% |
| !!! | AATTTATTTTGGTTTGTGAA+AGG | + | chr4.3:46936203-46936222 | None:intergenic | 20.0% |
| !!! | AATTTTGCTTACATTAGATT+TGG | + | chr4.3:46936955-46936974 | None:intergenic | 20.0% |
| !!! | TCAAATGTTGAATTTGTTAT+AGG | - | chr4.3:46936099-46936118 | MS.gene66753:CDS | 20.0% |
| !!! | TTAAGTTTTGGAATAAACTA+TGG | - | chr4.3:46935958-46935977 | MS.gene66753:CDS | 20.0% |
| ! | AAATAATAAGGAACCTGAAA+TGG | + | chr4.3:46935838-46935857 | None:intergenic | 25.0% |
| ! | AGTTTAAAGATACCTCATTA+GGG | + | chr4.3:46936515-46936534 | None:intergenic | 25.0% |
| ! | AGTTTATAACATTGGATTAC+AGG | - | chr4.3:46936902-46936921 | MS.gene66753:CDS | 25.0% |
| ! | ATATAAAGATAACCCTAATG+AGG | - | chr4.3:46936500-46936519 | MS.gene66753:CDS | 25.0% |
| ! | CAAAATGCAAAATTGTACAA+TGG | - | chr4.3:46936726-46936745 | MS.gene66753:CDS | 25.0% |
| ! | GATTCATTTAAGCAAATCAA+TGG | - | chr4.3:46935934-46935953 | MS.gene66753:CDS | 25.0% |
| ! | GCAAAAACATATACATCAAT+TGG | + | chr4.3:46936807-46936826 | None:intergenic | 25.0% |
| ! | GTTTATAACATTGGATTACA+GGG | - | chr4.3:46936903-46936922 | MS.gene66753:CDS | 25.0% |
| ! | TAGTTTAAAGATACCTCATT+AGG | + | chr4.3:46936516-46936535 | None:intergenic | 25.0% |
| ! | TCATAATGCAAATTTGTGTT+TGG | + | chr4.3:46936573-46936592 | None:intergenic | 25.0% |
| ! | TCATCAAGAAATTCAACAAT+TGG | + | chr4.3:46936438-46936457 | None:intergenic | 25.0% |
| ! | TGTAATCCAATGTTATAAAC+TGG | + | chr4.3:46936903-46936922 | None:intergenic | 25.0% |
| ! | TTATACCAATGTTGAAGTTA+AGG | - | chr4.3:46936647-46936666 | MS.gene66753:CDS | 25.0% |
| !! | AAATTTGCTTAGAAGAATTG+AGG | - | chr4.3:46936749-46936768 | MS.gene66753:CDS | 25.0% |
| !! | AGCTCATTCTTTTAACATTT+TGG | - | chr4.3:46936353-46936372 | MS.gene66753:CDS | 25.0% |
| !! | GATTAATTTTTATGTACCGA+CGG | - | chr4.3:46936990-46937009 | MS.gene66753:intron | 25.0% |
| !! | TTATGAACATTTTGCATTGA+AGG | + | chr4.3:46936288-46936307 | None:intergenic | 25.0% |
| !! | TTGTACAATTTTGCATTTTG+TGG | + | chr4.3:46936726-46936745 | None:intergenic | 25.0% |
| !! | TTTAATGAAGATTTGAAACC+TGG | - | chr4.3:46936831-46936850 | MS.gene66753:CDS | 25.0% |
| !!! | GTTGAATTTGTTATAGGAAT+TGG | - | chr4.3:46936105-46936124 | MS.gene66753:CDS | 25.0% |
| AAGTGATTTGAAGAAGGAAA+AGG | + | chr4.3:46935799-46935818 | None:intergenic | 30.0% | |
| AGAAACCTTAACTTCAACAT+TGG | + | chr4.3:46936655-46936674 | None:intergenic | 30.0% | |
| CATAATTCTCTTGTTAAGCT+TGG | - | chr4.3:46936303-46936322 | MS.gene66753:CDS | 30.0% | |
| CTGTTGTAACAGTAATTACT+TGG | + | chr4.3:46936335-46936354 | None:intergenic | 30.0% | |
| GAAAAGAAGTGATTTGAAGA+AGG | + | chr4.3:46935805-46935824 | None:intergenic | 30.0% | |
| GCATTATTTCAACCAAATGA+AGG | - | chr4.3:46936537-46936556 | MS.gene66753:CDS | 30.0% | |
| TATACATCAATTGGAACCAA+TGG | + | chr4.3:46936798-46936817 | None:intergenic | 30.0% | |
| TATGGACTTTATTATCCTGA+TGG | - | chr4.3:46936873-46936892 | MS.gene66753:CDS | 30.0% | |
| TTGCTTACATTAGATTTGGA+TGG | + | chr4.3:46936951-46936970 | None:intergenic | 30.0% | |
| ! | CAAATCAATGGCTTAAGTTT+TGG | - | chr4.3:46935946-46935965 | MS.gene66753:CDS | 30.0% |
| !!! | ATCCAATTTTGAGCTTTAGA+AGG | + | chr4.3:46936159-46936178 | None:intergenic | 30.0% |
| !!! | ATTTTGGTTTGTGAAAGGTA+AGG | + | chr4.3:46936198-46936217 | None:intergenic | 30.0% |
| !!! | TCCAATTTTGAGCTTTAGAA+GGG | + | chr4.3:46936158-46936177 | None:intergenic | 30.0% |
| AGAAGAATTGAGGAAAGACA+TGG | - | chr4.3:46936759-46936778 | MS.gene66753:CDS | 35.0% | |
| CAAATCTGACCTAAAAGAAC+CGG | + | chr4.3:46936400-46936419 | None:intergenic | 35.0% | |
| GAGAAAGCAAGTAGAACATT+TGG | + | chr4.3:46936075-46936094 | None:intergenic | 35.0% | |
| GGATCAACTAAACCTTCATT+TGG | + | chr4.3:46936552-46936571 | None:intergenic | 35.0% | |
| GGTAGACCAGTTTATAACAT+TGG | - | chr4.3:46936894-46936913 | MS.gene66753:CDS | 35.0% | |
| TATGCTGCAATCAAAGTGAT+AGG | - | chr4.3:46936624-46936643 | MS.gene66753:CDS | 35.0% | |
| TCTTGAATGAGATGGTAGAT+TGG | + | chr4.3:46935995-46936014 | None:intergenic | 35.0% | |
| TTCTTTCTTCTCACCATTTC+AGG | - | chr4.3:46935822-46935841 | MS.gene66753:intron | 35.0% | |
| ! | TTATAAACTGGTCTACCATC+AGG | + | chr4.3:46936891-46936910 | None:intergenic | 35.0% |
| !! | ACCCTTCTAAAGCTCAAAAT+TGG | - | chr4.3:46936154-46936173 | MS.gene66753:CDS | 35.0% |
| AATTCTTACCCTCCTTCATC+CGG | - | chr4.3:46936378-46936397 | MS.gene66753:CDS | 40.0% | |
| ACAGGGTTATCTACCACAAA+TGG | - | chr4.3:46936920-46936939 | MS.gene66753:CDS | 40.0% | |
| ACCTGCATCAAATCACATCT+CGG | + | chr4.3:46937072-46937091 | None:intergenic | 40.0% | |
| ACTGCAACTCTTGAATGAGA+TGG | + | chr4.3:46936003-46936022 | None:intergenic | 40.0% | |
| CCTGCTTCAGAAAGAAACTA+TGG | - | chr4.3:46936855-46936874 | MS.gene66753:CDS | 40.0% | |
| TAAAAGAACCGGATGAAGGA+GGG | + | chr4.3:46936389-46936408 | None:intergenic | 40.0% | |
| TGGTGTAGCTCCAATTTCAT+CGG | + | chr4.3:46936706-46936725 | None:intergenic | 40.0% | |
| TTAAAAAACGTGCACACCGT+CGG | + | chr4.3:46937009-46937028 | None:intergenic | 40.0% | |
| ! | ATTGGAACCAATGGATTTCC+AGG | + | chr4.3:46936789-46936808 | None:intergenic | 40.0% |
| ! | TCACATCTCGGAGATGTTTT+AGG | + | chr4.3:46937060-46937079 | None:intergenic | 40.0% |
| !! | CCATAGTTTCTTTCTGAAGC+AGG | + | chr4.3:46936858-46936877 | None:intergenic | 40.0% |
| !! | GAAGTTAAGGTTTCTGAGAC+AGG | - | chr4.3:46936660-46936679 | MS.gene66753:CDS | 40.0% |
| !! | TTAAGGTTTCTGAGACAGGT+TGG | - | chr4.3:46936664-46936683 | MS.gene66753:CDS | 40.0% |
| AATGGCGACACCGATGAAAT+TGG | - | chr4.3:46936693-46936712 | MS.gene66753:CDS | 45.0% | |
| CTAAAAGAACCGGATGAAGG+AGG | + | chr4.3:46936390-46936409 | None:intergenic | 45.0% | |
| GACCTAAAAGAACCGGATGA+AGG | + | chr4.3:46936393-46936412 | None:intergenic | 45.0% | |
| TCCGAGATGTGATTTGATGC+AGG | - | chr4.3:46937068-46937087 | MS.gene66753:CDS | 45.0% | |
| TGGAACTCCTGGAAATCCAT+TGG | - | chr4.3:46936779-46936798 | MS.gene66753:CDS | 45.0% | |
| TTGGATGGCACAACCATTTG+TGG | + | chr4.3:46936936-46936955 | None:intergenic | 45.0% | |
| ! | CTCCTTCATCCGGTTCTTTT+AGG | - | chr4.3:46936388-46936407 | MS.gene66753:CDS | 45.0% |
| !! | TTTCTTTCTGAAGCAGGACC+AGG | + | chr4.3:46936852-46936871 | None:intergenic | 45.0% |
| GAGGAAAGACATGGAACTCC+TGG | - | chr4.3:46936768-46936787 | MS.gene66753:CDS | 50.0% | |
| !! | GAGACAGGTTGGCCTTCAAA+TGG | - | chr4.3:46936675-46936694 | MS.gene66753:CDS | 50.0% |
| !! | TCATCGGTGTCGCCATTTGA+AGG | + | chr4.3:46936690-46936709 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 46935789 | 46937095 | 46935789 | ID=MS.gene66753 |
| chr4.3 | mRNA | 46935789 | 46937095 | 46935789 | ID=MS.gene66753.t1;Parent=MS.gene66753 |
| chr4.3 | exon | 46937041 | 46937095 | 46937041 | ID=MS.gene66753.t1.exon1;Parent=MS.gene66753.t1 |
| chr4.3 | CDS | 46937041 | 46937095 | 46937041 | ID=cds.MS.gene66753.t1;Parent=MS.gene66753.t1 |
| chr4.3 | exon | 46935922 | 46936949 | 46935922 | ID=MS.gene66753.t1.exon2;Parent=MS.gene66753.t1 |
| chr4.3 | CDS | 46935922 | 46936949 | 46935922 | ID=cds.MS.gene66753.t1;Parent=MS.gene66753.t1 |
| chr4.3 | exon | 46935789 | 46935794 | 46935789 | ID=MS.gene66753.t1.exon3;Parent=MS.gene66753.t1 |
| chr4.3 | CDS | 46935789 | 46935794 | 46935789 | ID=cds.MS.gene66753.t1;Parent=MS.gene66753.t1 |
| Gene Sequence |
| Protein sequence |
