AlfalfaGEDB | MS.gene66753

M. sativa cultivar XinJiangDaYe / MS.gene66753

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004553 GO:0005975 GO:0008150 GO:0008152 GO:0016787 GO:0016798 GO:0044238 GO:0071704
PFAM: PF00332.21

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66753.t1	XP_013455825.1	97.2	362	10	0	1	362	1	362	5.40E-202	713.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66753.t1	Q9ZQG9	63.2	356	127	2	6	360	9	361	2.0e-136	486.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66753.t1	A0A072UKG4	97.2	362	10	0	1	362	1	362	3.9e-202	713.4

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049661	MS.gene66753	0.806001	1.02E-49	-1.69E-46
MS.gene049662	MS.gene66753	0.801009	1.10E-48	-1.69E-46
MS.gene052340	MS.gene66753	0.83264	8.43E-56	-1.69E-46
MS.gene055116	MS.gene66753	0.841209	5.46E-58	-1.69E-46
MS.gene056191	MS.gene66753	0.811628	6.37E-51	-1.69E-46
MS.gene05702	MS.gene66753	0.836772	7.69E-57	-1.69E-46
MS.gene057749	MS.gene66753	0.825116	5.58E-54	-1.69E-46
MS.gene057823	MS.gene66753	0.811646	6.31E-51	-1.69E-46
MS.gene058002	MS.gene66753	0.801243	9.87E-49	-1.69E-46
MS.gene058710	MS.gene66753	0.800023	1.75E-48	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene063164	MS.gene66753	PPI
MS.gene064895	MS.gene66753	PPI
MS.gene66753	MS.gene45685	PPI
MS.gene66753	MS.gene003763	PPI
MS.gene66753	MS.gene68221	PPI
MS.gene66753	MS.gene014280	PPI
MS.gene66753	MS.gene50078	PPI
MS.gene66753	MS.gene056701	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66753.t1	MTR_4g052970	97.238	362	10	0	1	362	1	362	0.0	722
MS.gene66753.t1	MTR_2g086530	65.489	368	119	2	1	362	1	366	6.33e-176	494
MS.gene66753.t1	MTR_2g086530	67.638	343	103	2	26	362	5	345	3.37e-171	481
MS.gene66753.t1	MTR_1g077990	59.062	320	130	1	28	347	30	348	1.56e-140	405
MS.gene66753.t1	MTR_4g084480	51.320	341	164	1	10	350	15	353	2.35e-128	374
MS.gene66753.t1	MTR_4g084480	51.320	341	164	1	10	350	8	346	2.43e-128	373
MS.gene66753.t1	MTR_4g084480	51.320	341	164	1	10	350	15	353	2.94e-128	374
MS.gene66753.t1	MTR_2g019345	49.563	343	170	2	5	347	2	341	6.43e-123	360
MS.gene66753.t1	MTR_4g010200	43.820	356	194	2	9	358	8	363	1.98e-110	328
MS.gene66753.t1	MTR_4g114850	47.368	323	169	1	28	349	25	347	2.60e-109	324

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66753.t1	AT2G27500	64.118	340	121	1	22	360	22	361	1.88e-172	486
MS.gene66753.t1	AT2G27500	64.118	340	121	1	22	360	22	361	2.57e-172	484
MS.gene66753.t1	AT2G27500	63.251	283	103	1	79	360	1	283	1.06e-141	404
MS.gene66753.t1	AT1G32860	53.868	349	159	1	1	347	1	349	2.89e-139	402
MS.gene66753.t1	AT5G42100	53.704	324	147	2	28	348	26	349	5.98e-125	366
MS.gene66753.t1	AT5G42100	53.704	324	147	2	28	348	26	349	6.67e-125	366
MS.gene66753.t1	AT4G18340	48.276	348	177	2	3	347	5	352	4.58e-122	358
MS.gene66753.t1	AT4G18340	48.276	348	177	2	3	347	5	352	5.49e-122	357
MS.gene66753.t1	AT1G30080	48.235	340	171	2	10	346	16	353	1.31e-116	343
MS.gene66753.t1	AT1G30080	48.235	340	171	2	10	346	16	353	1.82e-116	344

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Find 58 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAAATTGCATAACTGT+TGG	0.468767	4.3:-46936649	MS.gene66753:CDS
AAATAATAAGGAACCTGAAA+TGG	0.423025	4.3:+46937027	None:intergenic
AAATTTGCTTAGAAGAATTG+AGG	0.496626	4.3:-46936113	MS.gene66753:CDS
AACAAATTCAACATTTGATT+TGG	0.238369	4.3:+46936768	None:intergenic
AACAGTTATGCAATTTATTT+TGG	0.148253	4.3:+46936651	None:intergenic
AAGTGATTTGAAGAAGGAAA+AGG	0.449912	4.3:+46937066	None:intergenic
AATGGCGACACCGATGAAAT+TGG	0.322115	4.3:-46936169	MS.gene66753:CDS
AATTCTTACCCTCCTTCATC+CGG	0.452830	4.3:-46936484	MS.gene66753:CDS
ACAGGGTTATCTACCACAAA+TGG	0.472431	4.3:-46935942	MS.gene66753:CDS
ACCCTTCTAAAGCTCAAAAT+TGG	0.203490	4.3:-46936708	MS.gene66753:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGTGATTTGAAGAAGGAAA+AGG	+	chr4.3:46935799-46935818	None:intergenic	30.0%
AGAAACCTTAACTTCAACAT+TGG	+	chr4.3:46936655-46936674	None:intergenic	30.0%
CATAATTCTCTTGTTAAGCT+TGG	-	chr4.3:46936303-46936322	MS.gene66753:CDS	30.0%
CTGTTGTAACAGTAATTACT+TGG	+	chr4.3:46936335-46936354	None:intergenic	30.0%
GAAAAGAAGTGATTTGAAGA+AGG	+	chr4.3:46935805-46935824	None:intergenic	30.0%
GCATTATTTCAACCAAATGA+AGG	-	chr4.3:46936537-46936556	MS.gene66753:CDS	30.0%
TATACATCAATTGGAACCAA+TGG	+	chr4.3:46936798-46936817	None:intergenic	30.0%
TATGGACTTTATTATCCTGA+TGG	-	chr4.3:46936873-46936892	MS.gene66753:CDS	30.0%
TTGCTTACATTAGATTTGGA+TGG	+	chr4.3:46936951-46936970	None:intergenic	30.0%
AGAAGAATTGAGGAAAGACA+TGG	-	chr4.3:46936759-46936778	MS.gene66753:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	46935789	46937095	46935789	ID=MS.gene66753
chr4.3	mRNA	46935789	46937095	46935789	ID=MS.gene66753.t1;Parent=MS.gene66753
chr4.3	exon	46937041	46937095	46937041	ID=MS.gene66753.t1.exon1;Parent=MS.gene66753.t1
chr4.3	CDS	46937041	46937095	46937041	ID=cds.MS.gene66753.t1;Parent=MS.gene66753.t1
chr4.3	exon	46935922	46936949	46935922	ID=MS.gene66753.t1.exon2;Parent=MS.gene66753.t1
chr4.3	CDS	46935922	46936949	46935922	ID=cds.MS.gene66753.t1;Parent=MS.gene66753.t1
chr4.3	exon	46935789	46935794	46935789	ID=MS.gene66753.t1.exon3;Parent=MS.gene66753.t1
chr4.3	CDS	46935789	46935794	46935789	ID=cds.MS.gene66753.t1;Parent=MS.gene66753.t1

Gene Sequence

>MS.gene66753

ATGACAACCTTTTCCTTCTTCAAATCACTTCTTTTCTTTCTTCTCACCATTTCAGGTTCCTTATTATTTATCTTCTCTCTACTATGCATTTTGTATTATGATATTTTTTTTTGTCTATTCTAATGTTTCTAAATATATGATTTCAGATTCATTTAAGCAAATCAATGGCTTAAGTTTTGGAATAAACTATGGACAAATAGCAACCAATCTACCATCTCATTCAAGAGTTGCAGTTCTTATAAGATCAATGAATGTGACAAGAATCAAACTCTATGATGCTGATCCAAATGTTCTACTTGCTTTCTCCAAATCAAATGTTGAATTTGTTATAGGAATTGGAAATGAGCATTTACAAAATATGACAAACCCTTCTAAAGCTCAAAATTGGATTCAACATCATGTTCTACCTTACCTTTCACAAACCAAAATAAATTGCATAACTGTTGGAAATGAAGTATTCAATAGCAATGATACTCAACTAATGTTGAATCTTCTACCTTCAATGCAAAATGTTCATAATTCTCTTGTTAAGCTTGGATTAGACCAAGTAATTACTGTTACAACAGCTCATTCTTTTAACATTTTGGATAATTCTTACCCTCCTTCATCCGGTTCTTTTAGGTCAGATTTGATTCAATATATTCAACCAATTGTTGAATTTCTTGATGAAATCAAATCATCTTTTCATATCAATGCTTACCCTTTTTTTGCATATAAAGATAACCCTAATGAGGTATCTTTAAACTATGCATTATTTCAACCAAATGAAGGTTTAGTTGATCCAAACACAAATTTGCATTATGATAACATGTTGTATGCTCAAATTGATGCTGTTTATGCTGCAATCAAAGTGATAGGTTATACCAATGTTGAAGTTAAGGTTTCTGAGACAGGTTGGCCTTCAAATGGCGACACCGATGAAATTGGAGCTACACCACAAAATGCAAAATTGTACAATGGAAATTTGCTTAGAAGAATTGAGGAAAGACATGGAACTCCTGGAAATCCATTGGTTCCAATTGATGTATATGTTTTTGCACTTTTTAATGAAGATTTGAAACCTGGTCCTGCTTCAGAAAGAAACTATGGACTTTATTATCCTGATGGTAGACCAGTTTATAACATTGGATTACAGGGTTATCTACCACAAATGGTTGTGCCATCCAAATCTAATGTAAGCAAAATTCTATTTACCTTTTGAGATTAATTTTTATGTACCGACGGTGTGCACGTTTTTTAAACGAATGATGTCACATTGTTATGTTATTCCTAAAACATCTCCGAGATGTGATTTGATGCAGGTGTAA

Protein sequence

>MS.gene66753.t1

MTTFSFFKSLLFFLLTISDSFKQINGLSFGINYGQIATNLPSHSRVAVLIRSMNVTRIKLYDADPNVLLAFSKSNVEFVIGIGNEHLQNMTNPSKAQNWIQHHVLPYLSQTKINCITVGNEVFNSNDTQLMLNLLPSMQNVHNSLVKLGLDQVITVTTAHSFNILDNSYPPSSGSFRSDLIQYIQPIVEFLDEIKSSFHINAYPFFAYKDNPNEVSLNYALFQPNEGLVDPNTNLHYDNMLYAQIDAVYAAIKVIGYTNVEVKVSETGWPSNGDTDEIGATPQNAKLYNGNLLRRIEERHGTPGNPLVPIDVYVFALFNEDLKPGPASERNYGLYYPDGRPVYNIGLQGYLPQMVVPSKSNV