AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69030.t1 MTR_6g088320 96.139 259 10 0 1 259 30 288 0.0 532
MS.gene69030.t1 MTR_8g102910 77.606 259 58 0 1 259 26 284 1.45e-160 447
MS.gene69030.t1 MTR_4g128410 57.087 254 103 2 3 254 31 280 1.48e-102 300
MS.gene69030.t1 MTR_7g093530 57.087 254 103 2 3 254 30 279 1.13e-100 295
MS.gene69030.t1 MTR_2g038860 54.440 259 115 2 3 258 32 290 6.30e-100 293
MS.gene69030.t1 MTR_2g038705 54.440 259 115 2 3 258 32 290 6.30e-100 293
MS.gene69030.t1 MTR_4g128400 57.087 254 103 2 3 254 34 283 1.96e-99 292
MS.gene69030.t1 MTR_4g126920 54.331 254 111 2 3 254 31 281 4.78e-99 291
MS.gene69030.t1 MTR_4g128570 56.299 254 91 3 3 254 37 272 2.70e-97 286
MS.gene69030.t1 MTR_4g128580 56.589 258 102 4 3 254 32 285 8.18e-97 285
MS.gene69030.t1 MTR_3g084630 53.282 259 109 3 3 259 34 282 1.39e-93 277
MS.gene69030.t1 MTR_3g084620 53.282 259 109 3 3 259 34 282 1.81e-93 276
MS.gene69030.t1 MTR_7g084760 50.579 259 122 4 2 258 37 291 2.59e-93 276
MS.gene69030.t1 MTR_7g084770 50.980 255 119 4 2 254 37 287 2.83e-93 276
MS.gene69030.t1 MTR_7g084750 50.593 253 119 4 4 254 39 287 3.15e-92 274
MS.gene69030.t1 MTR_4g128590 53.488 258 95 4 3 254 37 275 8.50e-91 270
MS.gene69030.t1 MTR_8g081040 53.150 254 108 3 3 254 37 281 1.27e-90 270
MS.gene69030.t1 MTR_2g089140 48.638 257 126 2 5 256 33 288 2.47e-90 269
MS.gene69030.t1 MTR_6g033085 50.988 253 118 4 4 254 39 287 8.81e-89 265
MS.gene69030.t1 MTR_6g033055 50.198 253 120 4 4 254 39 287 1.36e-88 265
MS.gene69030.t1 MTR_4g045853 48.438 256 126 2 4 254 34 288 1.90e-87 262
MS.gene69030.t1 MTR_4g097640 48.462 260 126 5 1 257 36 290 1.08e-86 259
MS.gene69030.t1 MTR_4g097670 48.462 260 126 5 1 257 36 290 1.08e-86 259
MS.gene69030.t1 MTR_4g097700 49.027 257 123 5 2 255 74 325 1.30e-86 261
MS.gene69030.t1 MTR_1g105900 46.094 256 134 2 1 254 38 291 2.35e-84 254
MS.gene69030.t1 MTR_2g089140 50.442 226 106 2 36 256 1 225 1.48e-82 247
MS.gene69030.t1 MTR_3g089540 50.000 244 115 1 13 256 48 284 4.21e-82 248
MS.gene69030.t1 MTR_8g102220 48.062 258 110 4 3 258 35 270 5.99e-82 247
MS.gene69030.t1 MTR_8g013055 48.438 256 123 6 3 255 37 286 8.88e-79 239
MS.gene69030.t1 MTR_5g091730 46.888 241 119 2 14 254 47 278 3.20e-77 236
MS.gene69030.t1 MTR_1g105495 45.136 257 133 4 3 254 41 294 1.46e-76 234
MS.gene69030.t1 MTR_7g056090 40.392 255 138 7 3 254 38 281 6.29e-62 196
MS.gene69030.t1 MTR_4g057450 39.147 258 138 6 5 254 58 304 2.26e-59 190
MS.gene69030.t1 MTR_1g063020 40.310 258 135 6 5 254 48 294 2.86e-59 190
MS.gene69030.t1 MTR_7g102260 40.310 258 135 7 5 254 45 291 1.82e-58 187
MS.gene69030.t1 MTR_2g095800 39.768 259 136 7 5 254 62 309 2.53e-58 188
MS.gene69030.t1 MTR_2g049130 42.678 239 127 7 5 240 37 268 7.21e-58 186
MS.gene69030.t1 MTR_2g049030 39.526 253 136 8 5 254 38 276 1.73e-55 180
MS.gene69030.t1 MTR_8g064180 35.458 251 153 5 11 254 62 310 2.52e-49 165
MS.gene69030.t1 MTR_1g033420 35.741 263 151 8 2 254 37 291 1.99e-48 163
MS.gene69030.t1 MTR_5g029100 37.450 251 143 8 13 254 60 305 2.87e-45 155
MS.gene69030.t1 MTR_8g090100 32.453 265 152 10 5 254 29 281 5.54e-42 146
MS.gene69030.t1 MTR_5g029100 38.496 226 125 8 38 254 54 274 5.98e-42 146
MS.gene69030.t1 MTR_8g479340 43.878 98 52 2 157 254 41 135 1.35e-20 85.5
MS.gene69030.t1 MTR_8g032640 34.706 170 103 4 17 184 33 196 7.55e-17 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69030.t1 AT4G28850 66.279 258 86 1 3 259 35 292 6.55e-136 385
MS.gene69030.t1 AT5G57530 58.661 254 99 3 3 254 33 282 2.23e-101 297
MS.gene69030.t1 AT5G57540 56.693 254 104 3 3 254 32 281 2.07e-98 289
MS.gene69030.t1 AT5G57550 56.299 254 102 4 3 254 36 282 7.19e-97 285
MS.gene69030.t1 AT5G57560 54.440 259 111 4 3 257 29 284 1.98e-96 284
MS.gene69030.t1 AT3G23730 51.550 258 123 1 3 258 33 290 6.97e-96 283
MS.gene69030.t1 AT2G18800 54.135 266 105 5 3 254 34 296 9.66e-96 283
MS.gene69030.t1 AT4G25810 54.651 258 111 3 3 257 32 286 1.88e-95 282
MS.gene69030.t1 AT4G14130 50.379 264 114 3 3 258 34 288 3.49e-93 276
MS.gene69030.t1 AT4G25820 53.257 261 102 4 3 254 36 285 1.64e-92 275
MS.gene69030.t1 AT4G30270 51.807 249 102 2 6 254 35 265 1.11e-90 269
MS.gene69030.t1 AT4G03210 49.004 251 126 2 4 254 36 284 5.07e-89 266
MS.gene69030.t1 AT5G13870 49.804 255 122 4 2 254 37 287 1.56e-88 265
MS.gene69030.t1 AT5G13870 49.804 255 122 4 2 254 37 287 1.56e-88 265
MS.gene69030.t1 AT5G13870 49.804 255 122 4 2 254 37 287 1.56e-88 265
MS.gene69030.t1 AT5G48070 51.867 241 108 3 14 254 49 281 5.11e-87 260
MS.gene69030.t1 AT2G06850 49.802 253 121 4 4 254 42 290 2.26e-86 259
MS.gene69030.t1 AT4G37800 49.412 255 121 5 3 254 40 289 1.92e-84 254
MS.gene69030.t1 AT2G14620 46.875 256 132 2 1 254 41 294 4.90e-83 251
MS.gene69030.t1 AT4G30290 50.980 255 114 4 3 254 30 276 6.42e-83 249
MS.gene69030.t1 AT5G65730 48.221 253 123 5 5 254 43 290 7.55e-83 250
MS.gene69030.t1 AT4G03210 50.667 225 109 2 30 254 34 256 1.69e-82 248
MS.gene69030.t1 AT2G06850 50.435 230 110 3 25 254 5 230 3.63e-81 244
MS.gene69030.t1 AT4G30280 51.092 229 104 3 26 254 61 281 3.50e-80 243
MS.gene69030.t1 AT2G14620 49.153 236 115 2 19 254 14 244 4.49e-80 241
MS.gene69030.t1 AT2G14620 50.000 222 109 1 33 254 14 233 2.94e-79 239
MS.gene69030.t1 AT1G65310 50.655 229 105 3 26 254 61 281 1.65e-78 239
MS.gene69030.t1 AT1G65310 51.304 230 102 4 26 254 83 303 1.84e-78 239
MS.gene69030.t1 AT4G13090 47.451 255 127 5 3 254 38 288 6.47e-78 238
MS.gene69030.t1 AT1G11545 44.402 259 134 5 3 254 44 299 4.02e-75 231
MS.gene69030.t1 AT3G25050 45.703 256 130 7 3 255 41 290 4.72e-75 230
MS.gene69030.t1 AT4G13080 45.882 255 127 6 3 254 45 291 1.67e-73 226
MS.gene69030.t1 AT3G44990 39.535 258 137 7 5 254 47 293 9.65e-60 191
MS.gene69030.t1 AT2G36870 40.385 260 136 7 5 256 51 299 5.24e-58 187
MS.gene69030.t1 AT1G10550 36.398 261 153 6 5 254 51 309 4.95e-51 169
MS.gene69030.t1 AT1G32170 37.405 262 144 10 5 254 40 293 3.14e-48 163
MS.gene69030.t1 AT3G48580 36.220 254 142 9 2 254 39 273 8.78e-48 160
MS.gene69030.t1 AT2G01850 35.081 248 147 6 14 254 50 290 4.59e-47 159
MS.gene69030.t1 AT1G14720 35.135 259 152 7 5 254 39 290 8.42e-47 159
MS.gene69030.t1 AT4G18990 35.178 253 147 5 17 254 62 312 1.11e-45 157
MS.gene69030.t1 AT3G48580 34.783 230 131 8 25 254 4 214 2.84e-41 141
MS.gene69030.t1 AT4G18990 35.821 201 115 4 66 254 40 238 3.33e-39 138
MS.gene69030.t1 AT2G36870 46.875 128 60 2 5 124 51 178 4.63e-33 119
MS.gene69030.t1 AT3G48580 32.283 254 132 10 2 254 39 253 9.92e-33 120

Find 65 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCCTAAAATGATTGAATT+TGG 0.149775 6.2:+86490115 None:intergenic
ACAAAGATACCATTCTTATT+TGG 0.154842 6.2:-86491530 MS.gene69030:CDS
TGAAAGTTTACACAAGTTTA+TGG 0.162367 6.2:-86490205 MS.gene69030:CDS
CGTGATACCAAAAGATTTAA+TGG 0.220099 6.2:-86489936 MS.gene69030:CDS
CTCAATTAGGTCAAATGAAT+TGG 0.230910 6.2:-86489992 MS.gene69030:CDS
TAGTTTCTGAATACCCTAAT+TGG 0.268420 6.2:+86490266 None:intergenic
ACTTTCATTCCTTGCTTGTT+TGG 0.270123 6.2:+86490221 None:intergenic
CATTTGACCATTAAATCTTT+TGG 0.275194 6.2:+86489929 None:intergenic
CTTTAAATATAGCTTCAAAT+TGG 0.283566 6.2:-86490049 MS.gene69030:CDS
CTTTCATTCCTTGCTTGTTT+GGG 0.284686 6.2:+86490222 None:intergenic
AATTGTGCCTTGAAACATTC+AGG 0.294572 6.2:+86489903 None:intergenic
ATATTGATAGTATACCAATT+AGG 0.297472 6.2:-86490280 MS.gene69030:CDS
GTGGACTTGTTAAGACAAAT+TGG 0.298616 6.2:-86490157 MS.gene69030:CDS
TTAGGGCAAGAGCTTGTAAA+TGG 0.303223 6.2:-86490100 MS.gene69030:CDS
TGCTCAACATGCATTACTTC+AGG 0.315377 6.2:-86492143 MS.gene69030:CDS
TTCTCCTACATACAAACAAT+TGG 0.317516 6.2:-86490021 MS.gene69030:CDS
CAATTGGGTTATGCTCAATT+AGG 0.347787 6.2:-86490005 MS.gene69030:CDS
CAATTTGATTCGTGATTCAA+TGG 0.364662 6.2:+86491505 None:intergenic
CTTGTGTTGGATAAAACTTC+AGG 0.376738 6.2:-86492106 MS.gene69030:intron
CATTGAATCACGAATCAAAT+TGG 0.377433 6.2:-86491504 MS.gene69030:CDS
GTTTATGGAATGCAGATGAT+TGG 0.389692 6.2:-86490190 MS.gene69030:CDS
TATAGTTGAACTTACATAGT+AGG 0.393405 6.2:+86491451 None:intergenic
AATGTATACACACAAGGAAA+TGG 0.402004 6.2:-86491263 MS.gene69030:CDS
TATTGATAGTATACCAATTA+GGG 0.404217 6.2:-86490279 MS.gene69030:CDS
GAGTTCTTAGGAAATAGTTC+AGG 0.427126 6.2:-86491308 MS.gene69030:CDS
ACATTTGTATGAACAATGTA+AGG 0.437791 6.2:+86491281 None:intergenic
CCAAAAGCATGTATATTACA+TGG 0.438084 6.2:-86492168 None:intergenic
TAAATATAGCTTCAAATTGG+TGG 0.446334 6.2:-86490046 MS.gene69030:CDS
TAACCCAATTGTTTGTATGT+AGG 0.448771 6.2:+86490017 None:intergenic
ATCAACTTACACAATTTCAG+TGG 0.449618 6.2:+86491166 None:intergenic
AATGCCTCCTGAATGTTTCA+AGG 0.456192 6.2:-86489910 MS.gene69030:CDS
TTCATAACTATACTATCCAT+TGG 0.462357 6.2:-86491193 MS.gene69030:CDS
CTTAACAAGTCCACCTCTTG+TGG 0.471870 6.2:+86490166 None:intergenic
TTTATGGAATGCAGATGATT+GGG 0.473254 6.2:-86490189 MS.gene69030:CDS
TCAATTAGGTCAAATGAATT+GGG 0.474336 6.2:-86489991 MS.gene69030:CDS
TTGGTCCCTGGTAATTCTGC+AGG 0.498046 6.2:-86491485 MS.gene69030:CDS
TCTCCTACATACAAACAATT+GGG 0.500434 6.2:-86490020 MS.gene69030:CDS
ATTCAGAAACTATGAAAATG+AGG 0.505200 6.2:-86490255 MS.gene69030:CDS
CAATTTCAGTGGGGTTCCAA+TGG 0.505427 6.2:+86491177 None:intergenic
ACCGTTCCTGCAGAATTACC+AGG 0.506719 6.2:+86491479 None:intergenic
TGATTGAATTTGGCTACAAA+TGG 0.508974 6.2:+86490125 None:intergenic
CTCATCATGTTGGCTTCCTG+TGG 0.509663 6.2:+86491340 None:intergenic
TAGTTATGAAAATCAGCAGT+TGG 0.519542 6.2:+86491206 None:intergenic
TCAATGGTTCCAAATAAGAA+TGG 0.531417 6.2:+86491521 None:intergenic
ATCATGTTGGCTTCCTGTGG+AGG 0.534512 6.2:+86491343 None:intergenic
TCAACTTACACAATTTCAGT+GGG 0.537978 6.2:+86491167 None:intergenic
CCCTGGTAATTCTGCAGGAA+CGG 0.538451 6.2:-86491480 MS.gene69030:CDS
TGAAGATCTCCAACTTGTGT+TGG 0.549817 6.2:-86492119 MS.gene69030:CDS
AGTTATGAAAATCAGCAGTT+GGG 0.554006 6.2:+86491207 None:intergenic
GCTCAACATGCATTACTTCA+GGG 0.554741 6.2:-86492142 MS.gene69030:CDS
AAGCTATATTTAAAGCACAT+TGG 0.557304 6.2:+86490057 None:intergenic
CAAAAGCATGTATATTACAT+GGG 0.559154 6.2:-86492167 None:intergenic
GATGATTGGGCCACAAGAGG+TGG 0.559220 6.2:-86490176 MS.gene69030:CDS
TCACGAATCAAATTGGTCCC+TGG 0.562979 6.2:-86491497 MS.gene69030:CDS
GCAAGAGCTTGTAAATGGAA+TGG 0.563028 6.2:-86490095 MS.gene69030:CDS
CAAAATCTATCTCATCATGT+TGG 0.568146 6.2:+86491330 None:intergenic
GCAGATGATTGGGCCACAAG+AGG 0.605501 6.2:-86490179 MS.gene69030:CDS
TTCAGAAACTATGAAAATGA+GGG 0.605928 6.2:-86490254 MS.gene69030:CDS
TGTGTGCAGCTATCCTCCAC+AGG 0.610193 6.2:-86491356 MS.gene69030:intron
ATTGCATACCCAAACAAGCA+AGG 0.626861 6.2:-86490230 MS.gene69030:CDS
CATACAAATGTATACACACA+AGG 0.631731 6.2:-86491269 MS.gene69030:CDS
AAAAGCATGTATATTACATG+GGG 0.648450 6.2:-86492166 None:intergenic
TGTGCCTTGAAACATTCAGG+AGG 0.693280 6.2:+86489906 None:intergenic
CAACTTACACAATTTCAGTG+GGG 0.709585 6.2:+86491168 None:intergenic
CCGTTCCTGCAGAATTACCA+GGG 0.722809 6.2:+86491480 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATAAATTTAAATTTACATA+AGG - chr6.2:86490646-86490665 MS.gene69030:intron 10.0%
!!! TTGATTAAATTTGTTATTAT+CGG + chr6.2:86490386-86490405 None:intergenic 10.0%
!! TGATATATAAAACATTAGAA+AGG + chr6.2:86491729-86491748 None:intergenic 15.0%
!!! AAACGTAAATTTTTATTAAG+TGG - chr6.2:86490996-86491015 MS.gene69030:intron 15.0%
!!! AACGTAAATTTTTATTAAGT+GGG - chr6.2:86490997-86491016 MS.gene69030:intron 15.0%
!!! CATATATAAAATTTGAAATG+CGG + chr6.2:86491201-86491220 None:intergenic 15.0%
!!! TGATTAAATTTGTTATTATC+GGG + chr6.2:86490385-86490404 None:intergenic 15.0%
!!! TTCTAATGTTTTATATATCA+TGG - chr6.2:86491729-86491748 MS.gene69030:intron 15.0%
!! AAAAATTTACGTTTAGTTGA+CGG + chr6.2:86490990-86491009 None:intergenic 20.0%
!! AAAATTAGAAAATAAGCATG+AGG - chr6.2:86491587-86491606 MS.gene69030:intron 20.0%
!! AAATTAGAAAATAAGCATGA+GGG - chr6.2:86491588-86491607 MS.gene69030:intron 20.0%
!! ACTTTCTTATAGAAATGAAA+AGG + chr6.2:86491696-86491715 None:intergenic 20.0%
!! CTTTAAATATAGCTTCAAAT+TGG - chr6.2:86492010-86492029 MS.gene69030:intron 20.0%
!! TCAACTTAACATGAAATATA+TGG - chr6.2:86490472-86490491 MS.gene69030:intron 20.0%
!! TTAATGTGTCAATTTGATAT+AGG + chr6.2:86491277-86491296 None:intergenic 20.0%
!!! ACGTAAATTTTTATTAAGTG+GGG - chr6.2:86490998-86491017 MS.gene69030:intron 20.0%
!!! ATATTGATAGTATACCAATT+AGG - chr6.2:86491779-86491798 MS.gene69030:intron 20.0%
!!! TATTGATAGTATACCAATTA+GGG - chr6.2:86491780-86491799 MS.gene69030:intron 20.0%
!!! TCATTTCTATAAGAAAGTAT+TGG - chr6.2:86491698-86491717 MS.gene69030:intron 20.0%
! ACAAAGATACCATTCTTATT+TGG - chr6.2:86490529-86490548 MS.gene69030:intron 25.0%
! ACATTTGTATGAACAATGTA+AGG + chr6.2:86490781-86490800 None:intergenic 25.0%
! ACTTAACATGAAATATATGG+TGG - chr6.2:86490475-86490494 MS.gene69030:intron 25.0%
! ATTCAGAAACTATGAAAATG+AGG - chr6.2:86491804-86491823 MS.gene69030:intron 25.0%
! TAAATATAGCTTCAAATTGG+TGG - chr6.2:86492013-86492032 MS.gene69030:intron 25.0%
! TATAGTTGAACTTACATAGT+AGG + chr6.2:86490611-86490630 None:intergenic 25.0%
! TCAATTAGGTCAAATGAATT+GGG - chr6.2:86492068-86492087 MS.gene69030:intron 25.0%
! TGAAAAAAAGTTCGTTACAT+TGG + chr6.2:86491322-86491341 None:intergenic 25.0%
! TGAAAGTTTACACAAGTTTA+TGG - chr6.2:86491854-86491873 MS.gene69030:intron 25.0%
! TTCAGAAACTATGAAAATGA+GGG - chr6.2:86491805-86491824 MS.gene69030:intron 25.0%
! TTCATAACTATACTATCCAT+TGG - chr6.2:86490866-86490885 MS.gene69030:intron 25.0%
!! AAGCTATATTTAAAGCACAT+TGG + chr6.2:86492005-86492024 None:intergenic 25.0%
!! AGCCAAATTCAATCATTTTA+GGG - chr6.2:86491942-86491961 MS.gene69030:intron 25.0%
!! CATTTGACCATTAAATCTTT+TGG + chr6.2:86492133-86492152 None:intergenic 25.0%
!! TAGCCAAATTCAATCATTTT+AGG - chr6.2:86491941-86491960 MS.gene69030:intron 25.0%
!! TAGTTGTAACTTTGAAAGTT+TGG - chr6.2:86490099-86490118 MS.gene69030:CDS 25.0%
!!! TTCACATTTTTTTTTACCAC+AGG - chr6.2:86491752-86491771 MS.gene69030:intron 25.0%
AAACTCTGCTAAGTATTCAT+TGG - chr6.2:86490924-86490943 MS.gene69030:intron 30.0%
AATGTATACACACAAGGAAA+TGG - chr6.2:86490796-86490815 MS.gene69030:intron 30.0%
ACCAACTGAGTTATGTTTAT+GGG - chr6.2:86491069-86491088 MS.gene69030:intron 30.0%
AGTTATGAAAATCAGCAGTT+GGG + chr6.2:86490855-86490874 None:intergenic 30.0%
ATAAACATAACTCAGTTGGT+AGG + chr6.2:86491069-86491088 None:intergenic 30.0%
ATCAACTTACACAATTTCAG+TGG + chr6.2:86490896-86490915 None:intergenic 30.0%
ATTACCCTTGAATCATATTG+TGG - chr6.2:86490237-86490256 MS.gene69030:CDS 30.0%
CAAAATCTATCTCATCATGT+TGG + chr6.2:86490732-86490751 None:intergenic 30.0%
CATACAAATGTATACACACA+AGG - chr6.2:86490790-86490809 MS.gene69030:intron 30.0%
CATTGAATCACGAATCAAAT+TGG - chr6.2:86490555-86490574 MS.gene69030:intron 30.0%
CGTGATACCAAAAGATTTAA+TGG - chr6.2:86492123-86492142 MS.gene69030:CDS 30.0%
CTCAATTAGGTCAAATGAAT+TGG - chr6.2:86492067-86492086 MS.gene69030:intron 30.0%
TAACCCAATTGTTTGTATGT+AGG + chr6.2:86492045-86492064 None:intergenic 30.0%
TACCAACTGAGTTATGTTTA+TGG - chr6.2:86491068-86491087 MS.gene69030:intron 30.0%
TAGTTATGAAAATCAGCAGT+TGG + chr6.2:86490856-86490875 None:intergenic 30.0%
TAGTTTCTGAATACCCTAAT+TGG + chr6.2:86491796-86491815 None:intergenic 30.0%
TATCCCACAATATGATTCAA+GGG + chr6.2:86490244-86490263 None:intergenic 30.0%
TCAACTTACACAATTTCAGT+GGG + chr6.2:86490895-86490914 None:intergenic 30.0%
TCAATGGTTCCAAATAAGAA+TGG + chr6.2:86490541-86490560 None:intergenic 30.0%
TCTCCTACATACAAACAATT+GGG - chr6.2:86492039-86492058 MS.gene69030:intron 30.0%
TGATCATTCATTCGTTTGTT+GGG + chr6.2:86490300-86490319 None:intergenic 30.0%
TGATTGAATTTGGCTACAAA+TGG + chr6.2:86491937-86491956 None:intergenic 30.0%
TGCCCTAAAATGATTGAATT+TGG + chr6.2:86491947-86491966 None:intergenic 30.0%
TTACCCTTGAATCATATTGT+GGG - chr6.2:86490238-86490257 MS.gene69030:CDS 30.0%
TTCTCCTACATACAAACAAT+TGG - chr6.2:86492038-86492057 MS.gene69030:intron 30.0%
TTGATCATTCATTCGTTTGT+TGG + chr6.2:86490301-86490320 None:intergenic 30.0%
TTGGTGCATCTTTCAATATT+TGG - chr6.2:86490118-86490137 MS.gene69030:CDS 30.0%
TTTATGGAATGCAGATGATT+GGG - chr6.2:86491870-86491889 MS.gene69030:intron 30.0%
! CAATTTGATTCGTGATTCAA+TGG + chr6.2:86490557-86490576 None:intergenic 30.0%
! GAGAGCAACAATTTTATCTT+TGG - chr6.2:86490824-86490843 MS.gene69030:intron 30.0%
! TAAACATAACTCAGTTGGTA+GGG + chr6.2:86491068-86491087 None:intergenic 30.0%
!! AAGCTAACATTTGAAGCTTT+TGG - chr6.2:86491463-86491482 MS.gene69030:intron 30.0%
!! AAGTTTTATCCAACACAAGT+TGG + chr6.2:86489952-86489971 None:intergenic 30.0%
!! AGCTAACATTTGAAGCTTTT+GGG - chr6.2:86491464-86491483 MS.gene69030:intron 30.0%
!!! ACATTTTTTTTTACCACAGG+TGG - chr6.2:86491755-86491774 MS.gene69030:intron 30.0%
!!! GAGATAGATTTTGAGTTCTT+AGG - chr6.2:86490739-86490758 MS.gene69030:intron 30.0%
AAACTAAACGGGCACTAAAT+TGG + chr6.2:86491389-86491408 None:intergenic 35.0%
AATTGTGCCTTGAAACATTC+AGG + chr6.2:86492159-86492178 None:intergenic 35.0%
AATTTAGTGCCCGTTTAGTT+TGG - chr6.2:86491388-86491407 MS.gene69030:intron 35.0%
ACTTGCATTGTTATATGCAG+GGG + chr6.2:86491045-86491064 None:intergenic 35.0%
ACTTTCATTCCTTGCTTGTT+TGG + chr6.2:86491841-86491860 None:intergenic 35.0%
ATGCAAACTCCAAACTAAAC+GGG + chr6.2:86491400-86491419 None:intergenic 35.0%
CAACTTACACAATTTCAGTG+GGG + chr6.2:86490894-86490913 None:intergenic 35.0%
CAATTGGGTTATGCTCAATT+AGG - chr6.2:86492054-86492073 MS.gene69030:intron 35.0%
CCAACTGAGTTATGTTTATG+GGG - chr6.2:86491070-86491089 MS.gene69030:intron 35.0%
CTATCCCACAATATGATTCA+AGG + chr6.2:86490245-86490264 None:intergenic 35.0%
CTTGTGTTGGATAAAACTTC+AGG - chr6.2:86489953-86489972 MS.gene69030:CDS 35.0%
CTTTCATTCCTTGCTTGTTT+GGG + chr6.2:86491840-86491859 None:intergenic 35.0%
GAATGAATGATCAAGCATGA+AGG - chr6.2:86490307-86490326 MS.gene69030:intron 35.0%
GACTTGCATTGTTATATGCA+GGG + chr6.2:86491046-86491065 None:intergenic 35.0%
GAGTTCTTAGGAAATAGTTC+AGG - chr6.2:86490751-86490770 MS.gene69030:intron 35.0%
GATGCAAACTCCAAACTAAA+CGG + chr6.2:86491401-86491420 None:intergenic 35.0%
GTTTATGGAATGCAGATGAT+TGG - chr6.2:86491869-86491888 MS.gene69030:intron 35.0%
TACTATCAATATACCACCTG+TGG + chr6.2:86491771-86491790 None:intergenic 35.0%
TGAATCATATTGTGGGATAG+CGG - chr6.2:86490245-86490264 MS.gene69030:CDS 35.0%
! AAGTGGGTTTATGGAAAAAG+TGG - chr6.2:86491490-86491509 MS.gene69030:CDS 35.0%
! TTTGGGTTAAAGTGGGTTTA+TGG - chr6.2:86491481-86491500 MS.gene69030:CDS 35.0%
!! GTCGCTTTAAAGTGTACATT+TGG + chr6.2:86491300-86491319 None:intergenic 35.0%
!! GTGGACTTGTTAAGACAAAT+TGG - chr6.2:86491902-86491921 MS.gene69030:intron 35.0%
!! TTTTATTAAGTGGGGTGTCT+GGG - chr6.2:86491006-86491025 MS.gene69030:intron 35.0%
!! TTTTTATTAAGTGGGGTGTC+TGG - chr6.2:86491005-86491024 MS.gene69030:intron 35.0%
!!! TGAAGCTTTTGGGTTAAAGT+GGG - chr6.2:86491474-86491493 MS.gene69030:CDS 35.0%
!!! TTGAAGCTTTTGGGTTAAAG+TGG - chr6.2:86491473-86491492 MS.gene69030:CDS 35.0%
AATGCCTCCTGAATGTTTCA+AGG - chr6.2:86492149-86492168 MS.gene69030:CDS 40.0%
ATTGCATACCCAAACAAGCA+AGG - chr6.2:86491829-86491848 MS.gene69030:intron 40.0%
CCCCATAAACATAACTCAGT+TGG + chr6.2:86491073-86491092 None:intergenic 40.0%
GAATCATATTGTGGGATAGC+GGG - chr6.2:86490246-86490265 MS.gene69030:CDS 40.0%
GAATGATCAAGCATGAAGGA+CGG - chr6.2:86490311-86490330 MS.gene69030:intron 40.0%
GCAAGAGCTTGTAAATGGAA+TGG - chr6.2:86491964-86491983 MS.gene69030:intron 40.0%
GCTCAACATGCATTACTTCA+GGG - chr6.2:86489917-86489936 MS.gene69030:CDS 40.0%
GGACTTGCATTGTTATATGC+AGG + chr6.2:86491047-86491066 None:intergenic 40.0%
TACTGATAAGTAGCTAGCCA+AGG + chr6.2:86490349-86490368 None:intergenic 40.0%
TGAAGATCTCCAACTTGTGT+TGG - chr6.2:86489940-86489959 MS.gene69030:CDS 40.0%
TGCTCAACATGCATTACTTC+AGG - chr6.2:86489916-86489935 MS.gene69030:CDS 40.0%
TTAGGGCAAGAGCTTGTAAA+TGG - chr6.2:86491959-86491978 MS.gene69030:intron 40.0%
! TTTATTAAGTGGGGTGTCTG+GGG - chr6.2:86491007-86491026 MS.gene69030:intron 40.0%
CAATTTCAGTGGGGTTCCAA+TGG + chr6.2:86490885-86490904 None:intergenic 45.0%
CATTGTTATATGCAGGGGTC+AGG + chr6.2:86491040-86491059 None:intergenic 45.0%
CTTAACAAGTCCACCTCTTG+TGG + chr6.2:86491896-86491915 None:intergenic 45.0%
TCACGAATCAAATTGGTCCC+TGG - chr6.2:86490562-86490581 MS.gene69030:intron 45.0%
TGTGCCTTGAAACATTCAGG+AGG + chr6.2:86492156-86492175 None:intergenic 45.0%
! TTCGTTTGTTGGGTTGTGAC+CGG + chr6.2:86490290-86490309 None:intergenic 45.0%
!! GGTGGTGTTACATGAATTGC+AGG - chr6.2:86490493-86490512 MS.gene69030:intron 45.0%
!! TTATATAATCTAATTAAATT+TGG + chr6.2:86490013-86490032 None:intergenic 5.0%
!!! AAAAATTTAATTTATTACAA+AGG + chr6.2:86490062-86490081 None:intergenic 5.0%
!!! TTTAAATTTATGTTATTTAT+TGG + chr6.2:86490638-86490657 None:intergenic 5.0%
ACCGTTCCTGCAGAATTACC+AGG + chr6.2:86490583-86490602 None:intergenic 50.0%
CCCTGGTAATTCTGCAGGAA+CGG - chr6.2:86490579-86490598 MS.gene69030:intron 50.0%
CCGTTCCTGCAGAATTACCA+GGG + chr6.2:86490582-86490601 None:intergenic 50.0%
GCTTTGCATACGTGCGTTTG+TGG + chr6.2:86491423-86491442 None:intergenic 50.0%
TTGGTCCCTGGTAATTCTGC+AGG - chr6.2:86490574-86490593 MS.gene69030:intron 50.0%
! TCGTTTGTTGGGTTGTGACC+GGG + chr6.2:86490289-86490308 None:intergenic 50.0%
!! ATCATGTTGGCTTCCTGTGG+AGG + chr6.2:86490719-86490738 None:intergenic 50.0%
!! CTCATCATGTTGGCTTCCTG+TGG + chr6.2:86490722-86490741 None:intergenic 50.0%
!! TCATATTGTGGGATAGCGGG+AGG - chr6.2:86490249-86490268 MS.gene69030:CDS 50.0%
AGTAGCTAGCCAAGGTCGCT+AGG + chr6.2:86490341-86490360 None:intergenic 55.0%
GATGATTGGGCCACAAGAGG+TGG - chr6.2:86491883-86491902 MS.gene69030:intron 55.0%
GCAGATGATTGGGCCACAAG+AGG - chr6.2:86491880-86491899 MS.gene69030:intron 55.0%
GTAGCTAGCCAAGGTCGCTA+GGG + chr6.2:86490340-86490359 None:intergenic 55.0%
TGTGTGCAGCTATCCTCCAC+AGG - chr6.2:86490703-86490722 MS.gene69030:intron 55.0%
! CGTTTGTTGGGTTGTGACCG+GGG + chr6.2:86490288-86490307 None:intergenic 55.0%
GACGGTCACCCTAGCGACCT+TGG - chr6.2:86490329-86490348 MS.gene69030:intron 65.0%
! GTTGGGTTGTGACCGGGGTG+CGG + chr6.2:86490283-86490302 None:intergenic 65.0%
! TTGGGTTGTGACCGGGGTGC+GGG + chr6.2:86490282-86490301 None:intergenic 65.0%
TGGGTTGTGACCGGGGTGCG+GGG + chr6.2:86490281-86490300 None:intergenic 70.0%
GAGGTTTCGCCCCCGCACCC+CGG - chr6.2:86490268-86490287 MS.gene69030:CDS 75.0%
GGGTTGTGACCGGGGTGCGG+GGG + chr6.2:86490280-86490299 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 86489899 86492182 86489899 ID=MS.gene69030
chr6.2 mRNA 86489899 86492182 86489899 ID=MS.gene69030.t1;Parent=MS.gene69030
chr6.2 exon 86492107 86492182 86492107 ID=MS.gene69030.t1.exon1;Parent=MS.gene69030.t1
chr6.2 CDS 86492107 86492182 86492107 ID=cds.MS.gene69030.t1;Parent=MS.gene69030.t1
chr6.2 exon 86491466 86491566 86491466 ID=MS.gene69030.t1.exon2;Parent=MS.gene69030.t1
chr6.2 CDS 86491466 86491566 86491466 ID=cds.MS.gene69030.t1;Parent=MS.gene69030.t1
chr6.2 exon 86491176 86491369 86491176 ID=MS.gene69030.t1.exon3;Parent=MS.gene69030.t1
chr6.2 CDS 86491176 86491369 86491176 ID=cds.MS.gene69030.t1;Parent=MS.gene69030.t1
chr6.2 exon 86489899 86490307 86489899 ID=MS.gene69030.t1.exon4;Parent=MS.gene69030.t1
chr6.2 CDS 86489899 86490307 86489899 ID=cds.MS.gene69030.t1;Parent=MS.gene69030.t1
Gene Sequence

>MS.gene69030

ATGTATATTACATGGGGTGCTCAACATGCATTACTTCAGGGTGAAGATCTCCAACTTGTGTTGGATAAAACTTCAGGTAGAATTAAATTTTGTACTTATATATAACTTGCACCAAATTTAATTAGATTATATAAAAAAAATTAATTAGATTATATGTTTGCCTTTGTAATAAATTAAATTTTTATAAAGAAAATAATTTATAGTTGTAACTTTGAAAGTTTGGTGCATCTTTCAATATTTGGCAATTGAATGTCAAATAAATGAAAAAAATTGTGATTCAAACTTTTTTTATCAATCAAACTTATGTTTTTTTTTTCATTGACGATTCAGTCTATAATATTACCCTTGAATCATATTGTGGGATAGCGGGAGGTTTCGCCCCCGCACCCCGGTCACAACCCAACAAACGAATGAATGATCAAGCATGAAGGACGGTCACCCTAGCGACCTTGGCTAGCTACTTATCAGTAATTATGAATCATACCCGATAATAACAAATTTAATCAATGTGTTCAAAAAAAAAAGTGTTAATTATATATTTGAATATAAATAAGTAAATGAATTTTTCTTGTGTCAACTTAACATGAAATATATGGTGGTGTTACATGAATTGCAGGATCTGCAGCTCAAACAAAGATACCATTCTTATTTGGAACCATTGAATCACGAATCAAATTGGTCCCTGGTAATTCTGCAGGAACGGTTACAGCCTACTATGTAAGTTCAACTATAAGATCCAATAAATAACATAAATTTAAATTTACATAAGGTAAAAAATATGCTAATGTTTCATTTTCATAAAAATGTGTGCAGCTATCCTCCACAGGAAGCCAACATGATGAGATAGATTTTGAGTTCTTAGGAAATAGTTCAGGACAACCTTACATTGTTCATACAAATGTATACACACAAGGAAATGGAAGTAGAGAGCAACAATTTTATCTTTGGTTTGACCCAACTGCTGATTTTCATAACTATACTATCCATTGGAACCCCACTGAAATTGTGTAAGTTGATAAAATACTAAACTCTGCTAAGTATTCATTGGCTTTAATTTTGATACACTATCATTATAAAGATTTTTTATACCGTCAACTAAACGTAAATTTTTATTAAGTGGGGTGTCTGGGGTTCGAATCCTGACCCCTGCATATAACAATGCAAGTCCCTACCAACTGAGTTATGTTTATGGGGACTTAAAAATGTTGACTTTAATCTTAATGATCTACATATGATATATTTTCAATAGTTAAACTTAAATATATCATATGACATTGTTGTTAACTGATTATTAATGATCCGCATTTCAAATTTTATATATGATTACAATTTTTAAGCATTTTGAAAGATGAAAATTCAGAGTTAAATATATTCTCCTATATCAAATTGACACATTAACCAAATGTACACTTTAAAGCGACCAATGTAACGAACTTTTTTTCAATTGATTGCAAAAGCAATCTCTAAATAGCTAAATGAGATTGTGTCCAATTTAGTGCCCGTTTAGTTTGGAGTTTGCATCCACAAACGCACGTATGCAAAGCAAAATTGTTGTGATTTCTAAAAGCTAACATTTGAAGCTTTTGGGTTAAAGTGGGTTTATGGAAAAAGTGGTTTGCAAAAAGCAGAAGCAAACAAACACTTAAAATTGATTTGCAAAAAGTCATTAAAATACTACTCACTATGTTAAAATTAGAAAATAAGCATGAGGGATATAGTACTTAAAAATCACATAAAATTATTTGTGATCAGTTGACATTGTAAAATATTATTTTGATAGTGCATATGATTTTTCCTTTTCATTTCTATAAGAAAGTATTGGTTACACCTTTCTAATGTTTTATATATCATGGTTCACATTTTTTTTTACCACAGGTGGTATATTGATAGTATACCAATTAGGGTATTCAGAAACTATGAAAATGAGGGCATTGCATACCCAAACAAGCAAGGAATGAAAGTTTACACAAGTTTATGGAATGCAGATGATTGGGCCACAAGAGGTGGACTTGTTAAGACAAATTGGACTAATGCACCATTTGTAGCCAAATTCAATCATTTTAGGGCAAGAGCTTGTAAATGGAATGGAGCAATTAGTATTAACCAATGTGCTTTAAATATAGCTTCAAATTGGTGGTCTTCTCCTACATACAAACAATTGGGTTATGCTCAATTAGGTCAAATGAATTGGGTCAGAAATAATTATATGATCTATGATTATTGTCGTGATACCAAAAGATTTAATGGTCAAATGCCTCCTGAATGTTTCAAGGCACAATTCTAA
Protein sequence

>MS.gene69030.t1

MYITWGAQHALLQGEDLQLVLDKTSGSAAQTKIPFLFGTIESRIKLVPGNSAGTVTAYYLSSTGSQHDEIDFEFLGNSSGQPYIVHTNVYTQGNGSREQQFYLWFDPTADFHNYTIHWNPTEIVWYIDSIPIRVFRNYENEGIAYPNKQGMKVYTSLWNADDWATRGGLVKTNWTNAPFVAKFNHFRARACKWNGAISINQCALNIASNWWSSPTYKQLGYAQLGQMNWVRNNYMIYDYCRDTKRFNGQMPPECFKAQF