Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44430.t1 | RHN63082.1 | 97.5 | 280 | 6 | 1 | 1 | 280 | 15 | 293 | 4.40E-167 | 597 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44430.t1 | Q8LER3 | 73.8 | 279 | 67 | 1 | 8 | 280 | 15 | 293 | 3.7e-130 | 465.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44430.t1 | A0A396IC01 | 97.5 | 280 | 6 | 1 | 1 | 280 | 15 | 293 | 3.2e-167 | 597.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene44430 | 0.807671 | 4.51E-50 | -1.69E-46 |
| MS.gene050122 | MS.gene44430 | 0.810447 | 1.15E-50 | -1.69E-46 |
| MS.gene050670 | MS.gene44430 | 0.812627 | 3.85E-51 | -1.69E-46 |
| MS.gene050926 | MS.gene44430 | -0.80036 | 1.49E-48 | -1.69E-46 |
| MS.gene051387 | MS.gene44430 | 0.807299 | 5.41E-50 | -1.69E-46 |
| MS.gene053301 | MS.gene44430 | 0.808793 | 2.60E-50 | -1.69E-46 |
| MS.gene053813 | MS.gene44430 | 0.814467 | 1.52E-51 | -1.69E-46 |
| MS.gene054435 | MS.gene44430 | -0.804686 | 1.92E-49 | -1.69E-46 |
| MS.gene054437 | MS.gene44430 | -0.806423 | 8.29E-50 | -1.69E-46 |
| MS.gene054447 | MS.gene44430 | -0.811327 | 7.40E-51 | -1.69E-46 |
| MS.gene054486 | MS.gene44430 | 0.832313 | 1.02E-55 | -1.69E-46 |
| MS.gene055486 | MS.gene44430 | 0.806868 | 6.67E-50 | -1.69E-46 |
| MS.gene055909 | MS.gene44430 | 0.836083 | 1.15E-56 | -1.69E-46 |
| MS.gene055911 | MS.gene44430 | 0.836614 | 8.44E-57 | -1.69E-46 |
| MS.gene056448 | MS.gene44430 | 0.808466 | 3.05E-50 | -1.69E-46 |
| MS.gene056511 | MS.gene44430 | 0.816108 | 6.55E-52 | -1.69E-46 |
| MS.gene057146 | MS.gene44430 | 0.800393 | 1.47E-48 | -1.69E-46 |
| MS.gene059368 | MS.gene44430 | 0.80928 | 2.05E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene44430 | 0.820599 | 6.30E-53 | -1.69E-46 |
| MS.gene059711 | MS.gene44430 | 0.837307 | 5.62E-57 | -1.69E-46 |
| MS.gene060837 | MS.gene44430 | 0.854215 | 1.43E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene44430 | MS.gene49973 | PPI |
| MS.gene44430 | MS.gene43303 | PPI |
| MS.gene44430 | MS.gene028653 | PPI |
| MS.gene44430 | MS.gene66956 | PPI |
| MS.gene44430 | MS.gene061468 | PPI |
| MS.gene44430 | MS.gene91400 | PPI |
| MS.gene44430 | MS.gene52293 | PPI |
| MS.gene44430 | MS.gene024330 | PPI |
| MS.gene44430 | MS.gene72238 | PPI |
| MS.gene44430 | MS.gene033759 | PPI |
| MS.gene44430 | MS.gene99665 | PPI |
| MS.gene44430 | MS.gene43849 | PPI |
| MS.gene44430 | MS.gene93417 | PPI |
| MS.gene44430 | MS.gene69030 | PPI |
| MS.gene049783 | MS.gene44430 | PPI |
| MS.gene44430 | MS.gene31221 | PPI |
| MS.gene44430 | MS.gene042579 | PPI |
| MS.gene44430 | MS.gene72278 | PPI |
| MS.gene44430 | MS.gene93839 | PPI |
| MS.gene033758 | MS.gene44430 | PPI |
| MS.gene44430 | MS.gene52294 | PPI |
| MS.gene52292 | MS.gene44430 | PPI |
| MS.gene44430 | MS.gene059595 | PPI |
| MS.gene44430 | MS.gene71577 | PPI |
| MS.gene44430 | MS.gene72276 | PPI |
| MS.gene44430 | MS.gene069844 | PPI |
| MS.gene44430 | MS.gene09167 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44430.t1 | MTR_4g097640 | 97.500 | 280 | 4 | 1 | 1 | 280 | 15 | 291 | 0.0 | 574 |
| MS.gene44430.t1 | MTR_4g097670 | 97.500 | 280 | 4 | 1 | 1 | 280 | 15 | 291 | 0.0 | 574 |
| MS.gene44430.t1 | MTR_4g097700 | 98.859 | 263 | 3 | 0 | 18 | 280 | 66 | 328 | 0.0 | 555 |
| MS.gene44430.t1 | MTR_7g084770 | 53.405 | 279 | 125 | 3 | 2 | 276 | 10 | 287 | 2.00e-104 | 306 |
| MS.gene44430.t1 | MTR_7g084760 | 55.253 | 257 | 113 | 2 | 21 | 276 | 32 | 287 | 1.08e-103 | 304 |
| MS.gene44430.t1 | MTR_6g033085 | 52.465 | 284 | 126 | 4 | 1 | 276 | 5 | 287 | 1.55e-102 | 301 |
| MS.gene44430.t1 | MTR_6g033055 | 52.688 | 279 | 123 | 4 | 6 | 276 | 10 | 287 | 4.63e-102 | 300 |
| MS.gene44430.t1 | MTR_7g084750 | 50.896 | 279 | 132 | 3 | 2 | 276 | 10 | 287 | 1.79e-101 | 298 |
| MS.gene44430.t1 | MTR_1g105900 | 53.668 | 259 | 116 | 4 | 21 | 276 | 34 | 291 | 3.38e-101 | 298 |
| MS.gene44430.t1 | MTR_2g089140 | 50.890 | 281 | 124 | 4 | 8 | 277 | 10 | 287 | 1.32e-100 | 296 |
| MS.gene44430.t1 | MTR_1g105495 | 53.053 | 262 | 119 | 2 | 19 | 276 | 33 | 294 | 1.03e-99 | 294 |
| MS.gene44430.t1 | MTR_4g045853 | 51.866 | 268 | 117 | 4 | 19 | 277 | 25 | 289 | 1.26e-99 | 293 |
| MS.gene44430.t1 | MTR_4g128410 | 49.286 | 280 | 132 | 6 | 1 | 276 | 7 | 280 | 4.21e-96 | 284 |
| MS.gene44430.t1 | MTR_4g126920 | 50.570 | 263 | 121 | 4 | 19 | 276 | 23 | 281 | 7.72e-94 | 278 |
| MS.gene44430.t1 | MTR_8g013055 | 52.308 | 260 | 117 | 5 | 21 | 277 | 31 | 286 | 1.71e-93 | 278 |
| MS.gene44430.t1 | MTR_7g093530 | 47.143 | 280 | 136 | 5 | 3 | 277 | 8 | 280 | 1.10e-92 | 275 |
| MS.gene44430.t1 | MTR_2g038860 | 45.423 | 284 | 142 | 4 | 4 | 278 | 9 | 288 | 1.25e-91 | 273 |
| MS.gene44430.t1 | MTR_2g038705 | 45.423 | 284 | 142 | 4 | 4 | 278 | 9 | 288 | 1.25e-91 | 273 |
| MS.gene44430.t1 | MTR_6g088320 | 48.239 | 284 | 136 | 6 | 1 | 279 | 9 | 286 | 5.46e-91 | 271 |
| MS.gene44430.t1 | MTR_4g128400 | 47.191 | 267 | 139 | 2 | 12 | 277 | 19 | 284 | 7.33e-91 | 271 |
| MS.gene44430.t1 | MTR_4g128570 | 48.188 | 276 | 127 | 4 | 2 | 276 | 12 | 272 | 8.64e-91 | 270 |
| MS.gene44430.t1 | MTR_4g128580 | 49.819 | 277 | 127 | 6 | 5 | 276 | 16 | 285 | 9.52e-91 | 271 |
| MS.gene44430.t1 | MTR_3g084630 | 48.375 | 277 | 133 | 4 | 1 | 276 | 10 | 277 | 1.41e-90 | 270 |
| MS.gene44430.t1 | MTR_3g084620 | 48.375 | 277 | 133 | 4 | 1 | 276 | 10 | 277 | 1.96e-90 | 270 |
| MS.gene44430.t1 | MTR_2g089140 | 54.667 | 225 | 92 | 2 | 62 | 277 | 1 | 224 | 4.16e-90 | 267 |
| MS.gene44430.t1 | MTR_8g081040 | 48.736 | 277 | 133 | 5 | 1 | 276 | 13 | 281 | 1.29e-89 | 268 |
| MS.gene44430.t1 | MTR_8g102910 | 50.379 | 264 | 123 | 5 | 19 | 277 | 20 | 280 | 1.36e-89 | 268 |
| MS.gene44430.t1 | MTR_4g128590 | 42.657 | 286 | 134 | 6 | 1 | 276 | 10 | 275 | 1.57e-78 | 239 |
| MS.gene44430.t1 | MTR_8g102220 | 44.404 | 277 | 132 | 5 | 1 | 276 | 11 | 266 | 2.16e-78 | 239 |
| MS.gene44430.t1 | MTR_7g056090 | 46.212 | 264 | 132 | 5 | 6 | 268 | 22 | 276 | 7.48e-78 | 238 |
| MS.gene44430.t1 | MTR_5g091730 | 42.960 | 277 | 149 | 4 | 1 | 276 | 10 | 278 | 5.36e-76 | 233 |
| MS.gene44430.t1 | MTR_3g089540 | 45.000 | 260 | 136 | 4 | 19 | 276 | 28 | 282 | 9.94e-71 | 220 |
| MS.gene44430.t1 | MTR_2g049030 | 41.107 | 253 | 138 | 7 | 21 | 270 | 30 | 274 | 1.86e-66 | 209 |
| MS.gene44430.t1 | MTR_2g049130 | 43.443 | 244 | 133 | 5 | 21 | 263 | 29 | 268 | 1.49e-65 | 206 |
| MS.gene44430.t1 | MTR_1g033420 | 38.246 | 285 | 166 | 7 | 1 | 277 | 10 | 292 | 6.65e-58 | 188 |
| MS.gene44430.t1 | MTR_5g029100 | 38.868 | 265 | 152 | 5 | 21 | 276 | 42 | 305 | 2.15e-57 | 187 |
| MS.gene44430.t1 | MTR_4g057450 | 37.209 | 258 | 147 | 6 | 18 | 268 | 47 | 296 | 4.87e-55 | 180 |
| MS.gene44430.t1 | MTR_2g095800 | 36.567 | 268 | 148 | 9 | 20 | 276 | 53 | 309 | 5.33e-54 | 177 |
| MS.gene44430.t1 | MTR_5g029100 | 37.544 | 285 | 158 | 7 | 1 | 276 | 1 | 274 | 8.45e-53 | 175 |
| MS.gene44430.t1 | MTR_8g064180 | 33.223 | 301 | 173 | 7 | 2 | 276 | 12 | 310 | 1.33e-51 | 171 |
| MS.gene44430.t1 | MTR_8g090100 | 36.194 | 268 | 154 | 7 | 21 | 277 | 21 | 282 | 1.85e-51 | 171 |
| MS.gene44430.t1 | MTR_1g063020 | 36.264 | 273 | 147 | 8 | 17 | 276 | 36 | 294 | 5.08e-50 | 167 |
| MS.gene44430.t1 | MTR_7g102260 | 36.742 | 264 | 150 | 8 | 21 | 276 | 37 | 291 | 5.51e-50 | 167 |
| MS.gene44430.t1 | MTR_8g479340 | 37.297 | 185 | 54 | 3 | 94 | 276 | 11 | 135 | 4.78e-32 | 115 |
| MS.gene44430.t1 | MTR_4g097650 | 92.157 | 51 | 1 | 1 | 1 | 51 | 15 | 62 | 2.33e-25 | 96.3 |
| MS.gene44430.t1 | MTR_8g032640 | 32.571 | 175 | 111 | 4 | 42 | 215 | 32 | 200 | 3.54e-17 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44430.t1 | AT5G65730 | 73.913 | 276 | 72 | 0 | 1 | 276 | 15 | 290 | 7.85e-162 | 451 |
| MS.gene44430.t1 | AT4G37800 | 73.835 | 279 | 67 | 1 | 8 | 280 | 15 | 293 | 9.48e-161 | 448 |
| MS.gene44430.t1 | AT4G03210 | 54.122 | 279 | 120 | 4 | 1 | 276 | 11 | 284 | 1.42e-104 | 306 |
| MS.gene44430.t1 | AT5G13870 | 50.883 | 283 | 129 | 4 | 1 | 276 | 8 | 287 | 4.59e-104 | 305 |
| MS.gene44430.t1 | AT5G13870 | 50.883 | 283 | 129 | 4 | 1 | 276 | 8 | 287 | 4.59e-104 | 305 |
| MS.gene44430.t1 | AT5G13870 | 50.883 | 283 | 129 | 4 | 1 | 276 | 8 | 287 | 4.59e-104 | 305 |
| MS.gene44430.t1 | AT2G14620 | 51.079 | 278 | 128 | 5 | 2 | 276 | 22 | 294 | 2.85e-101 | 298 |
| MS.gene44430.t1 | AT2G06850 | 51.064 | 282 | 131 | 3 | 1 | 276 | 10 | 290 | 8.28e-101 | 296 |
| MS.gene44430.t1 | AT1G11545 | 50.352 | 284 | 131 | 4 | 1 | 276 | 18 | 299 | 4.08e-100 | 295 |
| MS.gene44430.t1 | AT4G03210 | 57.143 | 245 | 98 | 3 | 35 | 276 | 16 | 256 | 1.33e-97 | 287 |
| MS.gene44430.t1 | AT3G23730 | 48.754 | 281 | 134 | 5 | 1 | 276 | 11 | 286 | 1.65e-95 | 283 |
| MS.gene44430.t1 | AT5G57550 | 48.201 | 278 | 137 | 4 | 1 | 276 | 10 | 282 | 2.05e-95 | 283 |
| MS.gene44430.t1 | AT2G06850 | 56.388 | 227 | 97 | 2 | 51 | 276 | 5 | 230 | 4.88e-92 | 272 |
| MS.gene44430.t1 | AT5G57560 | 46.853 | 286 | 134 | 5 | 2 | 279 | 9 | 284 | 5.93e-92 | 274 |
| MS.gene44430.t1 | AT4G14130 | 49.618 | 262 | 125 | 4 | 19 | 276 | 26 | 284 | 7.76e-91 | 271 |
| MS.gene44430.t1 | AT4G25810 | 49.064 | 267 | 124 | 5 | 19 | 278 | 24 | 285 | 4.90e-90 | 269 |
| MS.gene44430.t1 | AT4G30270 | 48.659 | 261 | 118 | 5 | 19 | 278 | 22 | 267 | 7.56e-89 | 265 |
| MS.gene44430.t1 | AT5G57530 | 48.669 | 263 | 125 | 5 | 19 | 276 | 25 | 282 | 7.63e-89 | 266 |
| MS.gene44430.t1 | AT4G13090 | 47.122 | 278 | 141 | 4 | 3 | 276 | 13 | 288 | 8.30e-89 | 266 |
| MS.gene44430.t1 | AT5G57540 | 47.909 | 263 | 127 | 5 | 19 | 276 | 24 | 281 | 1.11e-87 | 263 |
| MS.gene44430.t1 | AT2G14620 | 52.193 | 228 | 105 | 3 | 52 | 276 | 18 | 244 | 3.53e-87 | 260 |
| MS.gene44430.t1 | AT5G48070 | 45.977 | 261 | 132 | 6 | 19 | 276 | 27 | 281 | 3.79e-86 | 259 |
| MS.gene44430.t1 | AT2G14620 | 52.489 | 221 | 101 | 3 | 59 | 276 | 14 | 233 | 3.43e-85 | 255 |
| MS.gene44430.t1 | AT4G25820 | 47.529 | 263 | 128 | 4 | 19 | 276 | 28 | 285 | 3.84e-85 | 256 |
| MS.gene44430.t1 | AT2G18800 | 45.645 | 287 | 139 | 5 | 4 | 276 | 13 | 296 | 3.64e-84 | 254 |
| MS.gene44430.t1 | AT3G25050 | 43.816 | 283 | 148 | 5 | 1 | 276 | 11 | 289 | 2.16e-82 | 249 |
| MS.gene44430.t1 | AT4G13080 | 46.094 | 256 | 135 | 3 | 21 | 276 | 39 | 291 | 2.76e-82 | 249 |
| MS.gene44430.t1 | AT4G30290 | 44.803 | 279 | 143 | 7 | 1 | 276 | 6 | 276 | 2.99e-82 | 249 |
| MS.gene44430.t1 | AT4G28850 | 43.972 | 282 | 146 | 5 | 4 | 276 | 9 | 287 | 1.59e-81 | 248 |
| MS.gene44430.t1 | AT4G30280 | 43.728 | 279 | 146 | 7 | 1 | 276 | 11 | 281 | 2.31e-80 | 244 |
| MS.gene44430.t1 | AT1G65310 | 46.360 | 261 | 131 | 6 | 19 | 276 | 27 | 281 | 2.08e-78 | 239 |
| MS.gene44430.t1 | AT1G65310 | 46.360 | 261 | 131 | 6 | 19 | 276 | 49 | 303 | 2.59e-78 | 239 |
| MS.gene44430.t1 | AT1G32170 | 37.544 | 285 | 168 | 6 | 1 | 277 | 12 | 294 | 3.48e-58 | 189 |
| MS.gene44430.t1 | AT1G14720 | 38.516 | 283 | 166 | 6 | 1 | 277 | 11 | 291 | 1.23e-56 | 185 |
| MS.gene44430.t1 | AT3G48580 | 38.168 | 262 | 142 | 8 | 19 | 278 | 32 | 275 | 1.73e-53 | 175 |
| MS.gene44430.t1 | AT2G01850 | 36.170 | 282 | 174 | 5 | 1 | 277 | 11 | 291 | 3.77e-53 | 176 |
| MS.gene44430.t1 | AT1G10550 | 37.209 | 258 | 146 | 7 | 33 | 276 | 54 | 309 | 4.32e-53 | 175 |
| MS.gene44430.t1 | AT4G18990 | 35.395 | 291 | 163 | 7 | 10 | 277 | 25 | 313 | 2.94e-51 | 172 |
| MS.gene44430.t1 | AT3G44990 | 35.472 | 265 | 156 | 5 | 19 | 276 | 37 | 293 | 3.74e-51 | 170 |
| MS.gene44430.t1 | AT2G36870 | 36.431 | 269 | 144 | 10 | 21 | 276 | 43 | 297 | 6.21e-50 | 167 |
| MS.gene44430.t1 | AT3G48580 | 39.130 | 230 | 121 | 7 | 51 | 278 | 4 | 216 | 4.37e-48 | 159 |
| MS.gene44430.t1 | AT4G18990 | 37.500 | 200 | 106 | 5 | 95 | 277 | 42 | 239 | 3.84e-40 | 141 |
| MS.gene44430.t1 | AT3G48580 | 33.969 | 262 | 133 | 9 | 19 | 278 | 32 | 255 | 3.65e-38 | 135 |
| MS.gene44430.t1 | AT2G36870 | 41.304 | 138 | 72 | 4 | 21 | 151 | 43 | 178 | 6.28e-27 | 103 |
Find 64 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCCCATGGCTTGGATCTT+TGG | 0.186462 | 8.1:-24821258 | None:intergenic |
| CTTGATCAATTTGCTTGATA+TGG | 0.190810 | 8.1:-24820060 | None:intergenic |
| TTCACACTTACTCAATTCTT+TGG | 0.200130 | 8.1:+24820616 | MS.gene44430:CDS |
| CATTATGGGAAGCTGATAAT+TGG | 0.203518 | 8.1:+24821289 | MS.gene44430:CDS |
| ACCCCACATGAGTGTCTTTC+AGG | 0.215363 | 8.1:+24821555 | MS.gene44430:CDS |
| ATTGTTCTTGTACACTCTTA+TGG | 0.245745 | 8.1:-24821214 | None:intergenic |
| GTGGATTAGAGAAAATTAAT+TGG | 0.259471 | 8.1:+24821322 | MS.gene44430:CDS |
| CTTTATTTACTTACACAATA+TGG | 0.267037 | 8.1:-24820647 | None:intergenic |
| GAGAACAAAGAGTTAATCTT+TGG | 0.282944 | 8.1:+24820574 | MS.gene44430:CDS |
| CTCCCATGGCTTGGATCTTT+GGG | 0.285110 | 8.1:-24821257 | None:intergenic |
| TTGTTCTTGTACACTCTTAT+GGG | 0.317433 | 8.1:-24821213 | None:intergenic |
| TTGATCAATTTGCTTGATAT+GGG | 0.378785 | 8.1:-24820059 | None:intergenic |
| AGACACTCATGTGGGGTCAT+TGG | 0.379857 | 8.1:-24821549 | None:intergenic |
| AAGTCTTGATTAAAAGTTGC+TGG | 0.392991 | 8.1:-24820016 | None:intergenic |
| GACACTCATGTGGGGTCATT+GGG | 0.402289 | 8.1:-24821548 | None:intergenic |
| TCCAAGGTGAAGTACTTGTT+TGG | 0.404192 | 8.1:+24820282 | MS.gene44430:CDS |
| GGGTTAGTGGCACATGTAGT+TGG | 0.414336 | 8.1:-24821408 | None:intergenic |
| ATTGAAGGATGTGCAATTCC+AGG | 0.423415 | 8.1:+24821384 | MS.gene44430:CDS |
| CCTATACCTTCTAGCTTCAA+TGG | 0.438940 | 8.1:-24821472 | None:intergenic |
| TACAAGAACAATGAAGCAAA+AGG | 0.440360 | 8.1:+24821225 | MS.gene44430:CDS |
| AATTTGCTTGATATGGGGTT+CGG | 0.440864 | 8.1:-24820053 | None:intergenic |
| GCTTACCAGAGCCTTGGTCT+AGG | 0.449767 | 8.1:-24820102 | None:intergenic |
| ATGTTTGTTTGAACTGTGTA+AGG | 0.451638 | 8.1:-24820528 | None:intergenic |
| ATTATGGGAAGCTGATAATT+GGG | 0.454763 | 8.1:+24821290 | MS.gene44430:CDS |
| CAAACAAACATTTATGCTCA+TGG | 0.456844 | 8.1:+24820540 | MS.gene44430:CDS |
| AGGTTGCGGGTTTGCTTCCA+AGG | 0.460427 | 8.1:+24820266 | MS.gene44430:intron |
| AACATTTATGCTCATGGAAA+GGG | 0.466489 | 8.1:+24820546 | MS.gene44430:CDS |
| GAGTTCTTGGGAAACCGTAC+TGG | 0.469916 | 8.1:+24820501 | MS.gene44430:CDS |
| GTGGCACATGTAGTTGGTCC+TGG | 0.486861 | 8.1:-24821402 | None:intergenic |
| ATGGCTTGGATCTTTGGGTA+TGG | 0.489548 | 8.1:-24821252 | None:intergenic |
| ACCTTTCTATGCTTATTACA+AGG | 0.510047 | 8.1:+24821353 | MS.gene44430:CDS |
| TTAGTGCCATTGAAGCTAGA+AGG | 0.511286 | 8.1:+24821466 | MS.gene44430:CDS |
| AAACATTTATGCTCATGGAA+AGG | 0.519040 | 8.1:+24820545 | MS.gene44430:CDS |
| ATTATTGTCAAGATAAATCA+AGG | 0.521408 | 8.1:+24821523 | MS.gene44430:CDS |
| TTGAAAATACTCCCATGGCT+TGG | 0.522806 | 8.1:-24821266 | None:intergenic |
| CTGTGTAAGGTTGTCCAGTA+CGG | 0.533081 | 8.1:-24820515 | None:intergenic |
| CATATCAAGCAAATTGATCA+AGG | 0.537805 | 8.1:+24820061 | MS.gene44430:CDS |
| CTTACCAGAGCCTTGGTCTA+GGG | 0.545499 | 8.1:-24820101 | None:intergenic |
| CCATTGAAGCTAGAAGGTAT+AGG | 0.547043 | 8.1:+24821472 | MS.gene44430:CDS |
| TGATCAATTTGCTTGATATG+GGG | 0.550945 | 8.1:-24820058 | None:intergenic |
| CTTGTACCTGGAGATTCTGC+TGG | 0.563207 | 8.1:+24820327 | MS.gene44430:CDS |
| TCCTTGTAATAAGCATAGAA+AGG | 0.566971 | 8.1:-24821354 | None:intergenic |
| ACAAGAACAATGAAGCAAAA+GGG | 0.575105 | 8.1:+24821226 | MS.gene44430:CDS |
| TGACAGTGTTCGTGATGAGT+TGG | 0.575375 | 8.1:+24820473 | MS.gene44430:CDS |
| GATAATTGGGCAACAAGAGG+TGG | 0.576591 | 8.1:+24821303 | MS.gene44430:CDS |
| TTGAAGCTAGAAGGTATAGG+TGG | 0.577676 | 8.1:+24821475 | MS.gene44430:CDS |
| TAAGTGTGAAAGTCAGCAGA+AGG | 0.588490 | 8.1:-24820603 | None:intergenic |
| TATGTCGCTTACCAGAGCCT+TGG | 0.600698 | 8.1:-24820108 | None:intergenic |
| TAATGTTGAAAATACTCCCA+TGG | 0.606737 | 8.1:-24821271 | None:intergenic |
| GCCAAACAAGTACTTCACCT+TGG | 0.609031 | 8.1:-24820283 | None:intergenic |
| GACAATAATCATAGATCACA+TGG | 0.610597 | 8.1:-24821509 | None:intergenic |
| ACAGTACCAGCAGAATCTCC+AGG | 0.614116 | 8.1:-24820333 | None:intergenic |
| TGCCTGAAAGACACTCATGT+GGG | 0.615126 | 8.1:-24821557 | None:intergenic |
| ATCAAGACTTTCATGTCACG+TGG | 0.619539 | 8.1:+24820029 | MS.gene44430:CDS |
| GTTTGATCTTCATGCTAACA+CGG | 0.620390 | 8.1:-24820305 | None:intergenic |
| ATGAAGATCAAACTTGTACC+TGG | 0.621043 | 8.1:+24820315 | MS.gene44430:CDS |
| CTTACCCTAGACCAAGGCTC+TGG | 0.621562 | 8.1:+24820097 | MS.gene44430:CDS |
| TACCCAAAGATCCAAGCCAT+GGG | 0.632864 | 8.1:+24821255 | MS.gene44430:CDS |
| ATACCCAAAGATCCAAGCCA+TGG | 0.633713 | 8.1:+24821254 | MS.gene44430:CDS |
| GCTGATAATTGGGCAACAAG+AGG | 0.638048 | 8.1:+24821300 | MS.gene44430:CDS |
| TGAAGCTAGAAGGTATAGGT+GGG | 0.659236 | 8.1:+24821476 | MS.gene44430:CDS |
| GCCTGAAAGACACTCATGTG+GGG | 0.659861 | 8.1:-24821556 | None:intergenic |
| ATGCCTGAAAGACACTCATG+TGG | 0.662116 | 8.1:-24821558 | None:intergenic |
| ATTCAACTTACCCTAGACCA+AGG | 0.670711 | 8.1:+24820091 | MS.gene44430:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAAAGAAATTATCTAATTT+TGG | + | chr8.1:24821018-24821037 | MS.gene44430:intron | 10.0% |
| !!! | ATAATATTATATTCAGTTTT+TGG | - | chr8.1:24820425-24820444 | None:intergenic | 10.0% |
| !!! | TAATGAACTATAATATATTT+TGG | - | chr8.1:24820161-24820180 | None:intergenic | 10.0% |
| !! | AATACTACTATACTATAATT+TGG | + | chr8.1:24820194-24820213 | MS.gene44430:intron | 15.0% |
| !! | AGTTAAATACTTGAAAATTA+TGG | + | chr8.1:24820698-24820717 | MS.gene44430:intron | 15.0% |
| !!! | AAAAAAATACTTTGATTTTG+TGG | + | chr8.1:24821118-24821137 | MS.gene44430:intron | 15.0% |
| !!! | ATTTTGAAATTAAAGATAGT+CGG | - | chr8.1:24821000-24821019 | None:intergenic | 15.0% |
| !!! | TATAATATATTTTGGACATT+AGG | - | chr8.1:24820153-24820172 | None:intergenic | 15.0% |
| !! | ATTATTGTCAAGATAAATCA+AGG | + | chr8.1:24821523-24821542 | MS.gene44430:CDS | 20.0% |
| !! | CTTAATAATGCAAAGAATTA+TGG | + | chr8.1:24820887-24820906 | MS.gene44430:intron | 20.0% |
| !!! | AAATACTTAACTTTTTGCAA+AGG | + | chr8.1:24820246-24820265 | MS.gene44430:intron | 20.0% |
| !!! | CTTTATTTACTTACACAATA+TGG | - | chr8.1:24820650-24820669 | None:intergenic | 20.0% |
| ! | AGTATTTGTACTAGATAAGT+AGG | - | chr8.1:24820233-24820252 | None:intergenic | 25.0% |
| ! | ATGATGTTTCAAACAAAAAG+TGG | - | chr8.1:24820394-24820413 | None:intergenic | 25.0% |
| !! | TTGATCAATTTGCTTGATAT+GGG | - | chr8.1:24820062-24820081 | None:intergenic | 25.0% |
| !!! | GTGGATTAGAGAAAATTAAT+TGG | + | chr8.1:24821322-24821341 | MS.gene44430:CDS | 25.0% |
| !!! | TACAAGGATTTTGATATTGA+AGG | + | chr8.1:24821369-24821388 | MS.gene44430:CDS | 25.0% |
| !!! | TTCATCATGTGTTTTTGTTT+GGG | + | chr8.1:24819988-24820007 | MS.gene44430:CDS | 25.0% |
| !!! | TTTCATCATGTGTTTTTGTT+TGG | + | chr8.1:24819987-24820006 | MS.gene44430:CDS | 25.0% |
| AAACATTTATGCTCATGGAA+AGG | + | chr8.1:24820545-24820564 | MS.gene44430:CDS | 30.0% | |
| AAAGAATTATGGCTAGACAT+GGG | + | chr8.1:24820898-24820917 | MS.gene44430:intron | 30.0% | |
| AACAACTTTGACAACTAACT+CGG | - | chr8.1:24820839-24820858 | None:intergenic | 30.0% | |
| AACATTTATGCTCATGGAAA+GGG | + | chr8.1:24820546-24820565 | MS.gene44430:CDS | 30.0% | |
| AAGTCTTGATTAAAAGTTGC+TGG | - | chr8.1:24820019-24820038 | None:intergenic | 30.0% | |
| ACAAGAACAATGAAGCAAAA+GGG | + | chr8.1:24821226-24821245 | MS.gene44430:CDS | 30.0% | |
| ACCTTTCTATGCTTATTACA+AGG | + | chr8.1:24821353-24821372 | MS.gene44430:CDS | 30.0% | |
| ATGTTTGTTTGAACTGTGTA+AGG | - | chr8.1:24820531-24820550 | None:intergenic | 30.0% | |
| ATTATGGGAAGCTGATAATT+GGG | + | chr8.1:24821290-24821309 | MS.gene44430:CDS | 30.0% | |
| ATTGTTCTTGTACACTCTTA+TGG | - | chr8.1:24821217-24821236 | None:intergenic | 30.0% | |
| CAAACAAACATTTATGCTCA+TGG | + | chr8.1:24820540-24820559 | MS.gene44430:CDS | 30.0% | |
| CATATCAAGCAAATTGATCA+AGG | + | chr8.1:24820061-24820080 | MS.gene44430:CDS | 30.0% | |
| GACAACATTGTGTCAAAAAA+AGG | - | chr8.1:24821087-24821106 | None:intergenic | 30.0% | |
| GACAATAATCATAGATCACA+TGG | - | chr8.1:24821512-24821531 | None:intergenic | 30.0% | |
| GAGAACAAAGAGTTAATCTT+TGG | + | chr8.1:24820574-24820593 | MS.gene44430:CDS | 30.0% | |
| GGCTAACTACATTTAGTATA+TGG | - | chr8.1:24820806-24820825 | None:intergenic | 30.0% | |
| TAATGTTGAAAATACTCCCA+TGG | - | chr8.1:24821274-24821293 | None:intergenic | 30.0% | |
| TACAAGAACAATGAAGCAAA+AGG | + | chr8.1:24821225-24821244 | MS.gene44430:CDS | 30.0% | |
| TCCTTGTAATAAGCATAGAA+AGG | - | chr8.1:24821357-24821376 | None:intergenic | 30.0% | |
| TTCACACTTACTCAATTCTT+TGG | + | chr8.1:24820616-24820635 | MS.gene44430:CDS | 30.0% | |
| TTGTTCTTGTACACTCTTAT+GGG | - | chr8.1:24821216-24821235 | None:intergenic | 30.0% | |
| ! | CTTGATCAATTTGCTTGATA+TGG | - | chr8.1:24820063-24820082 | None:intergenic | 30.0% |
| ! | TGATCAATTTGCTTGATATG+GGG | - | chr8.1:24820061-24820080 | None:intergenic | 30.0% |
| ! | TTAACTTTTTGCAAAGGTTG+CGG | + | chr8.1:24820252-24820271 | MS.gene44430:intron | 30.0% |
| !! | TCATCATGTGTTTTTGTTTG+GGG | + | chr8.1:24819989-24820008 | MS.gene44430:CDS | 30.0% |
| !!! | GGGAGTATTTTCAACATTAT+GGG | + | chr8.1:24821275-24821294 | MS.gene44430:CDS | 30.0% |
| !!! | TGGGAGTATTTTCAACATTA+TGG | + | chr8.1:24821274-24821293 | MS.gene44430:CDS | 30.0% |
| AACTAACTCGGTTCAAATAG+TGG | - | chr8.1:24820827-24820846 | None:intergenic | 35.0% | |
| ATGAAGATCAAACTTGTACC+TGG | + | chr8.1:24820315-24820334 | MS.gene44430:CDS | 35.0% | |
| CAAAGAATTATGGCTAGACA+TGG | + | chr8.1:24820897-24820916 | MS.gene44430:intron | 35.0% | |
| CACTAACCCAAAAAATTGGT+GGG | + | chr8.1:24821422-24821441 | MS.gene44430:CDS | 35.0% | |
| CATTATGGGAAGCTGATAAT+TGG | + | chr8.1:24821289-24821308 | MS.gene44430:CDS | 35.0% | |
| CTAAGTGCTAACCACAAATT+TGG | - | chr8.1:24820733-24820752 | None:intergenic | 35.0% | |
| GTTTGATCTTCATGCTAACA+CGG | - | chr8.1:24820308-24820327 | None:intergenic | 35.0% | |
| ! | AATTTGCTTGATATGGGGTT+CGG | - | chr8.1:24820056-24820075 | None:intergenic | 35.0% |
| ! | TAACTTTTTGCAAAGGTTGC+GGG | + | chr8.1:24820253-24820272 | MS.gene44430:intron | 35.0% |
| !!! | GAGTTGGATTTTGAGTTCTT+GGG | + | chr8.1:24820489-24820508 | MS.gene44430:CDS | 35.0% |
| !!! | TGAGTTGGATTTTGAGTTCT+TGG | + | chr8.1:24820488-24820507 | MS.gene44430:CDS | 35.0% |
| ACCCAAAAAATTGGTGGGAA+AGG | + | chr8.1:24821427-24821446 | MS.gene44430:CDS | 40.0% | |
| AGCACTTAGCAGAGTTACAT+TGG | + | chr8.1:24820744-24820763 | MS.gene44430:intron | 40.0% | |
| ATAGAAGCTGCATGTGTGTA+AGG | - | chr8.1:24821181-24821200 | None:intergenic | 40.0% | |
| ATCAAGACTTTCATGTCACG+TGG | + | chr8.1:24820029-24820048 | MS.gene44430:CDS | 40.0% | |
| ATTCAACTTACCCTAGACCA+AGG | + | chr8.1:24820091-24820110 | MS.gene44430:CDS | 40.0% | |
| ATTGAAGGATGTGCAATTCC+AGG | + | chr8.1:24821384-24821403 | MS.gene44430:CDS | 40.0% | |
| CCACTAACCCAAAAAATTGG+TGG | + | chr8.1:24821421-24821440 | MS.gene44430:CDS | 40.0% | |
| CCCAAAAAATTGGTGGGAAA+GGG | + | chr8.1:24821428-24821447 | MS.gene44430:CDS | 40.0% | |
| GCTAACCACAAATTTGGACA+TGG | - | chr8.1:24820727-24820746 | None:intergenic | 40.0% | |
| GGAAACCATGTCCAAATTTG+TGG | + | chr8.1:24820719-24820738 | MS.gene44430:intron | 40.0% | |
| TAAGTGTGAAAGTCAGCAGA+AGG | - | chr8.1:24820606-24820625 | None:intergenic | 40.0% | |
| TTGAAAATACTCCCATGGCT+TGG | - | chr8.1:24821269-24821288 | None:intergenic | 40.0% | |
| ! | CCATTGAAGCTAGAAGGTAT+AGG | + | chr8.1:24821472-24821491 | MS.gene44430:CDS | 40.0% |
| ! | GTGCCACTAACCCAAAAAAT+TGG | + | chr8.1:24821418-24821437 | MS.gene44430:CDS | 40.0% |
| ! | TCCAAGGTGAAGTACTTGTT+TGG | + | chr8.1:24820282-24820301 | MS.gene44430:CDS | 40.0% |
| ! | TGAAGCTAGAAGGTATAGGT+GGG | + | chr8.1:24821476-24821495 | MS.gene44430:CDS | 40.0% |
| ! | TTAGTGCCATTGAAGCTAGA+AGG | + | chr8.1:24821466-24821485 | MS.gene44430:CDS | 40.0% |
| ! | TTGAAGCTAGAAGGTATAGG+TGG | + | chr8.1:24821475-24821494 | MS.gene44430:CDS | 40.0% |
| !! | CCTATACCTTCTAGCTTCAA+TGG | - | chr8.1:24821475-24821494 | None:intergenic | 40.0% |
| !!! | ACCCTTTCCCACCAATTTTT+TGG | - | chr8.1:24821432-24821451 | None:intergenic | 40.0% |
| !!! | CCACCAATTTTTTGGGTTAG+TGG | - | chr8.1:24821424-24821443 | None:intergenic | 40.0% |
| !!! | CCCTTTCCCACCAATTTTTT+GGG | - | chr8.1:24821431-24821450 | None:intergenic | 40.0% |
| ATACCCAAAGATCCAAGCCA+TGG | + | chr8.1:24821254-24821273 | MS.gene44430:CDS | 45.0% | |
| ATGCCTGAAAGACACTCATG+TGG | - | chr8.1:24821561-24821580 | None:intergenic | 45.0% | |
| CTGTGTAAGGTTGTCCAGTA+CGG | - | chr8.1:24820518-24820537 | None:intergenic | 45.0% | |
| GATAATTGGGCAACAAGAGG+TGG | + | chr8.1:24821303-24821322 | MS.gene44430:CDS | 45.0% | |
| GCCAAACAAGTACTTCACCT+TGG | - | chr8.1:24820286-24820305 | None:intergenic | 45.0% | |
| GCTGATAATTGGGCAACAAG+AGG | + | chr8.1:24821300-24821319 | MS.gene44430:CDS | 45.0% | |
| TACCCAAAGATCCAAGCCAT+GGG | + | chr8.1:24821255-24821274 | MS.gene44430:CDS | 45.0% | |
| TGACAGTGTTCGTGATGAGT+TGG | + | chr8.1:24820473-24820492 | MS.gene44430:CDS | 45.0% | |
| TGCCTGAAAGACACTCATGT+GGG | - | chr8.1:24821560-24821579 | None:intergenic | 45.0% | |
| ! | ATGGCTTGGATCTTTGGGTA+TGG | - | chr8.1:24821255-24821274 | None:intergenic | 45.0% |
| !!! | ATATAATATTATTTTTTTTG+TGG | + | chr8.1:24820433-24820452 | MS.gene44430:intron | 5.0% |
| ACAGTACCAGCAGAATCTCC+AGG | - | chr8.1:24820336-24820355 | None:intergenic | 50.0% | |
| ACCCCACATGAGTGTCTTTC+AGG | + | chr8.1:24821555-24821574 | MS.gene44430:CDS | 50.0% | |
| AGACACTCATGTGGGGTCAT+TGG | - | chr8.1:24821552-24821571 | None:intergenic | 50.0% | |
| CTTACCAGAGCCTTGGTCTA+GGG | - | chr8.1:24820104-24820123 | None:intergenic | 50.0% | |
| CTTGTACCTGGAGATTCTGC+TGG | + | chr8.1:24820327-24820346 | MS.gene44430:CDS | 50.0% | |
| GACACTCATGTGGGGTCATT+GGG | - | chr8.1:24821551-24821570 | None:intergenic | 50.0% | |
| GAGTTCTTGGGAAACCGTAC+TGG | + | chr8.1:24820501-24820520 | MS.gene44430:CDS | 50.0% | |
| GCCTGAAAGACACTCATGTG+GGG | - | chr8.1:24821559-24821578 | None:intergenic | 50.0% | |
| ! | ACTCCCATGGCTTGGATCTT+TGG | - | chr8.1:24821261-24821280 | None:intergenic | 50.0% |
| ! | CTCCCATGGCTTGGATCTTT+GGG | - | chr8.1:24821260-24821279 | None:intergenic | 50.0% |
| ! | GGGTTAGTGGCACATGTAGT+TGG | - | chr8.1:24821411-24821430 | None:intergenic | 50.0% |
| ! | TATGTCGCTTACCAGAGCCT+TGG | - | chr8.1:24820111-24820130 | None:intergenic | 50.0% |
| CTTACCCTAGACCAAGGCTC+TGG | + | chr8.1:24820097-24820116 | MS.gene44430:CDS | 55.0% | |
| GCTTACCAGAGCCTTGGTCT+AGG | - | chr8.1:24820105-24820124 | None:intergenic | 55.0% | |
| GTGGCACATGTAGTTGGTCC+TGG | - | chr8.1:24821405-24821424 | None:intergenic | 55.0% | |
| ! | AGGTTGCGGGTTTGCTTCCA+AGG | + | chr8.1:24820266-24820285 | MS.gene44430:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 24819965 | 24821584 | 24819965 | ID=MS.gene44430 |
| chr8.1 | mRNA | 24819965 | 24821584 | 24819965 | ID=MS.gene44430.t1;Parent=MS.gene44430 |
| chr8.1 | exon | 24819965 | 24820118 | 24819965 | ID=MS.gene44430.t1.exon1;Parent=MS.gene44430.t1 |
| chr8.1 | CDS | 24819965 | 24820118 | 24819965 | ID=cds.MS.gene44430.t1;Parent=MS.gene44430.t1 |
| chr8.1 | exon | 24820268 | 24820368 | 24820268 | ID=MS.gene44430.t1.exon2;Parent=MS.gene44430.t1 |
| chr8.1 | CDS | 24820268 | 24820368 | 24820268 | ID=cds.MS.gene44430.t1;Parent=MS.gene44430.t1 |
| chr8.1 | exon | 24820459 | 24820655 | 24820459 | ID=MS.gene44430.t1.exon3;Parent=MS.gene44430.t1 |
| chr8.1 | CDS | 24820459 | 24820655 | 24820459 | ID=cds.MS.gene44430.t1;Parent=MS.gene44430.t1 |
| chr8.1 | exon | 24821194 | 24821584 | 24821194 | ID=MS.gene44430.t1.exon4;Parent=MS.gene44430.t1 |
| chr8.1 | CDS | 24821194 | 24821584 | 24821194 | ID=cds.MS.gene44430.t1;Parent=MS.gene44430.t1 |
| Gene Sequence |
| Protein sequence |
