AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44431

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44431.t1 MTR_4g097640 98.282 291 5 0 1 291 1 291 0.0 606
MS.gene44431.t1 MTR_4g097670 98.282 291 5 0 1 291 1 291 0.0 606
MS.gene44431.t1 MTR_4g097700 93.471 291 7 1 1 291 50 328 0.0 569
MS.gene44431.t1 MTR_7g084770 53.047 279 123 3 16 287 10 287 1.25e-103 304
MS.gene44431.t1 MTR_7g084760 55.253 257 113 2 32 287 32 287 2.07e-103 303
MS.gene44431.t1 MTR_6g033085 52.500 280 126 3 14 287 9 287 3.46e-102 300
MS.gene44431.t1 MTR_6g033055 51.786 280 128 3 14 287 9 287 8.18e-102 300
MS.gene44431.t1 MTR_1g105900 52.190 274 126 5 17 287 20 291 9.34e-102 300
MS.gene44431.t1 MTR_4g045853 49.825 285 131 4 13 288 8 289 3.53e-101 298
MS.gene44431.t1 MTR_7g084750 53.307 257 118 2 32 287 32 287 3.85e-101 298
MS.gene44431.t1 MTR_2g089140 50.523 287 125 4 11 288 9 287 5.45e-101 297
MS.gene44431.t1 MTR_1g105495 50.699 286 130 3 13 287 9 294 1.94e-100 296
MS.gene44431.t1 MTR_4g128410 49.821 279 131 6 14 287 6 280 1.17e-96 286
MS.gene44431.t1 MTR_4g126920 50.570 263 121 4 30 287 23 281 1.19e-93 278
MS.gene44431.t1 MTR_8g013055 52.308 260 117 5 32 288 31 286 2.67e-93 278
MS.gene44431.t1 MTR_7g093530 48.000 275 132 5 20 288 11 280 1.54e-92 276
MS.gene44431.t1 MTR_4g128580 50.365 274 127 5 19 287 16 285 1.81e-91 273
MS.gene44431.t1 MTR_6g088320 47.703 283 140 5 13 290 7 286 2.48e-91 273
MS.gene44431.t1 MTR_2g038860 46.127 284 138 5 17 289 9 288 6.38e-91 272
MS.gene44431.t1 MTR_2g038705 46.127 284 138 5 17 289 9 288 6.38e-91 272
MS.gene44431.t1 MTR_3g084630 48.029 279 136 4 11 287 6 277 9.47e-91 271
MS.gene44431.t1 MTR_3g084620 48.029 279 136 4 11 287 6 277 1.02e-90 271
MS.gene44431.t1 MTR_4g128400 45.583 283 150 3 9 288 3 284 1.65e-90 271
MS.gene44431.t1 MTR_8g102910 48.540 274 133 5 20 288 10 280 3.94e-90 270
MS.gene44431.t1 MTR_2g089140 54.667 225 92 2 73 288 1 224 6.04e-90 267
MS.gene44431.t1 MTR_8g081040 48.410 283 138 5 7 287 5 281 7.58e-90 269
MS.gene44431.t1 MTR_4g128570 49.807 259 114 4 32 289 31 274 1.72e-89 268
MS.gene44431.t1 MTR_8g102220 44.727 275 131 5 15 287 11 266 1.71e-78 239
MS.gene44431.t1 MTR_7g056090 44.610 269 144 4 12 279 12 276 3.50e-78 239
MS.gene44431.t1 MTR_4g128590 42.123 292 136 6 9 287 4 275 8.51e-78 238
MS.gene44431.t1 MTR_5g091730 42.143 280 154 4 9 287 6 278 1.75e-74 230
MS.gene44431.t1 MTR_3g089540 45.000 260 136 4 30 287 28 282 7.88e-71 221
MS.gene44431.t1 MTR_2g049030 39.636 275 152 8 13 281 8 274 2.06e-66 209
MS.gene44431.t1 MTR_2g049130 43.443 244 133 5 32 274 29 268 2.91e-65 206
MS.gene44431.t1 MTR_1g033420 37.500 288 167 7 12 288 7 292 8.61e-58 188
MS.gene44431.t1 MTR_5g029100 38.868 265 152 5 32 287 42 305 7.29e-57 186
MS.gene44431.t1 MTR_4g057450 36.842 266 153 6 29 287 47 304 7.19e-55 180
MS.gene44431.t1 MTR_2g095800 35.048 311 165 12 3 287 10 309 5.76e-54 178
MS.gene44431.t1 MTR_8g064180 34.437 302 167 9 14 287 12 310 6.05e-52 172
MS.gene44431.t1 MTR_5g029100 40.948 232 127 5 65 287 44 274 6.90e-52 172
MS.gene44431.t1 MTR_8g090100 36.194 268 154 7 32 288 21 282 1.84e-51 172
MS.gene44431.t1 MTR_1g063020 36.264 273 147 8 28 287 36 294 4.94e-50 167
MS.gene44431.t1 MTR_7g102260 36.364 264 151 8 32 287 37 291 1.98e-49 166
MS.gene44431.t1 MTR_4g097650 91.935 62 5 0 1 62 1 62 3.09e-36 124
MS.gene44431.t1 MTR_8g479340 37.297 185 54 3 105 287 11 135 4.49e-32 116
MS.gene44431.t1 MTR_8g032640 32.571 175 111 4 53 226 32 200 3.79e-17 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44431.t1 AT5G65730 72.069 290 78 1 1 287 1 290 1.05e-162 454
MS.gene44431.t1 AT4G37800 72.509 291 75 2 4 291 5 293 4.44e-162 452
MS.gene44431.t1 AT5G13870 52.330 279 125 3 16 287 10 287 1.60e-105 309
MS.gene44431.t1 AT5G13870 52.330 279 125 3 16 287 10 287 1.60e-105 309
MS.gene44431.t1 AT5G13870 52.330 279 125 3 16 287 10 287 1.60e-105 309
MS.gene44431.t1 AT4G03210 53.069 277 124 3 14 287 11 284 2.52e-103 303
MS.gene44431.t1 AT1G11545 50.000 290 134 5 7 287 12 299 6.34e-101 298
MS.gene44431.t1 AT2G14620 51.273 275 129 5 16 287 22 294 8.87e-101 297
MS.gene44431.t1 AT2G06850 51.071 280 132 3 12 287 12 290 4.45e-100 295
MS.gene44431.t1 AT4G03210 56.175 251 103 3 40 287 10 256 1.11e-97 288
MS.gene44431.t1 AT3G23730 49.104 279 134 5 16 287 9 286 4.79e-95 282
MS.gene44431.t1 AT5G57550 50.769 260 121 4 30 287 28 282 6.57e-95 281
MS.gene44431.t1 AT5G57560 46.644 298 137 6 1 290 1 284 3.17e-92 275
MS.gene44431.t1 AT2G06850 56.388 227 97 2 62 287 5 230 7.51e-92 272
MS.gene44431.t1 AT4G14130 48.175 274 133 5 20 287 14 284 6.20e-91 272
MS.gene44431.t1 AT4G25810 47.368 285 135 6 12 289 9 285 1.88e-90 270
MS.gene44431.t1 AT4G30270 46.953 279 130 6 14 289 4 267 1.38e-89 268
MS.gene44431.t1 AT5G57530 48.669 263 125 5 30 287 25 282 6.57e-89 266
MS.gene44431.t1 AT4G13090 47.143 280 142 4 12 287 11 288 4.70e-88 265
MS.gene44431.t1 AT5G57540 47.909 263 127 5 30 287 24 281 1.48e-87 263
MS.gene44431.t1 AT2G14620 52.632 228 104 4 63 287 18 244 3.03e-87 261
MS.gene44431.t1 AT5G48070 44.326 282 146 7 11 287 6 281 8.08e-87 261
MS.gene44431.t1 AT2G14620 52.941 221 100 4 70 287 14 233 3.24e-85 255
MS.gene44431.t1 AT4G25820 46.182 275 134 5 18 287 20 285 3.80e-85 257
MS.gene44431.t1 AT2G18800 47.037 270 128 4 32 287 28 296 9.06e-84 254
MS.gene44431.t1 AT4G30290 45.487 277 142 6 14 287 6 276 2.31e-83 252
MS.gene44431.t1 AT4G13080 46.094 256 135 3 32 287 39 291 4.79e-82 249
MS.gene44431.t1 AT3G25050 42.955 291 145 6 14 287 3 289 1.04e-81 248
MS.gene44431.t1 AT4G30280 44.404 277 145 6 14 287 11 281 2.50e-81 247
MS.gene44431.t1 AT4G28850 45.833 264 134 4 30 287 27 287 4.51e-81 247
MS.gene44431.t1 AT1G65310 46.360 261 131 6 30 287 27 281 1.92e-78 239
MS.gene44431.t1 AT1G65310 46.360 261 131 6 30 287 49 303 2.90e-78 240
MS.gene44431.t1 AT1G14720 39.161 286 163 8 12 288 8 291 1.95e-57 187
MS.gene44431.t1 AT1G32170 37.201 293 171 7 7 288 4 294 5.92e-57 187
MS.gene44431.t1 AT2G01850 37.193 285 170 7 12 288 8 291 2.74e-54 179
MS.gene44431.t1 AT3G48580 38.168 262 142 8 30 289 32 275 4.45e-53 174
MS.gene44431.t1 AT1G10550 37.209 258 146 7 44 287 54 309 7.08e-53 175
MS.gene44431.t1 AT3G44990 35.849 265 155 5 30 287 37 293 6.16e-51 169
MS.gene44431.t1 AT4G18990 35.484 279 159 6 29 288 37 313 1.44e-50 171
MS.gene44431.t1 AT2G36870 36.431 269 144 10 32 287 43 297 8.33e-50 167
MS.gene44431.t1 AT3G48580 39.130 230 121 7 62 289 4 216 1.01e-47 159
MS.gene44431.t1 AT4G18990 37.500 200 106 5 106 288 42 239 6.13e-40 140
MS.gene44431.t1 AT3G48580 33.969 262 133 9 30 289 32 255 1.09e-37 134
MS.gene44431.t1 AT2G36870 41.304 138 72 4 32 162 43 178 8.97e-27 103

Find 64 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGATCAATTTGCTTGATA+TGG 0.190810 8.1:-24831078 None:intergenic
TTCATACTTACTCCATTCTT+TGG 0.196067 8.1:+24831636 MS.gene44431:CDS
CATTATGGGAAGCTGATAAT+TGG 0.203518 8.1:+24832592 MS.gene44431:CDS
ACCCCACATGAGTGTCTTTC+AGG 0.215363 8.1:+24832858 MS.gene44431:CDS
TTGTTCTTGTATACTCTAAT+AGG 0.231975 8.1:-24832516 None:intergenic
GTGGATTAGAGAAAATTAAT+TGG 0.259471 8.1:+24832625 MS.gene44431:CDS
GATTCTCGTATCATCTGTTT+GGG 0.264815 8.1:+24831006 MS.gene44431:CDS
ATATTCGTTTGAACTGTATA+TGG 0.285858 8.1:-24831548 None:intergenic
GAGAACAAAGGGTTAATCTT+TGG 0.288208 8.1:+24831594 MS.gene44431:CDS
GGATTCTCGTATCATCTGTT+TGG 0.292551 8.1:+24831005 MS.gene44431:CDS
AAGTCTTGATTAAAAGTTGC+TGG 0.378416 8.1:-24831034 None:intergenic
TTGATCAATTTGCTTGATAT+GGG 0.378785 8.1:-24831077 None:intergenic
AGACACTCATGTGGGGTCAT+TGG 0.379857 8.1:-24832852 None:intergenic
GAGTTCTTGGGAAACCGTAC+TGG 0.398600 8.1:+24831521 MS.gene44431:CDS
GACACTCATGTGGGGTCATT+GGG 0.402289 8.1:-24832851 None:intergenic
AGGTTGTGGGTTTGCTTCCA+AGG 0.412831 8.1:+24831282 MS.gene44431:intron
GGGTTAGTGGCACATGTAGT+TGG 0.414336 8.1:-24832711 None:intergenic
ATTGAAGGATGTGCAATTCC+AGG 0.423415 8.1:+24832687 MS.gene44431:CDS
TCCAAGGTGAAATACTTGTT+TGG 0.434603 8.1:+24831298 MS.gene44431:CDS
CAAACGAATATTTATGCTCA+TGG 0.438237 8.1:+24831560 MS.gene44431:CDS
CCTATACCTTCTAGCTTCAA+TGG 0.438940 8.1:-24832775 None:intergenic
TACAAGAACAATGAAGCAAA+AGG 0.440360 8.1:+24832528 MS.gene44431:CDS
ACTTACCAGAGCCTTGGTCT+AGG 0.447851 8.1:-24831120 None:intergenic
ATTATGGGAAGCTGATAATT+GGG 0.454763 8.1:+24832593 MS.gene44431:CDS
AATATTTATGCTCATGGAAA+GGG 0.474052 8.1:+24831566 MS.gene44431:CDS
GTGGCACATGTAGTTGGTCC+TGG 0.486861 8.1:-24832705 None:intergenic
CTTGTCCCTGGAGATTCTGC+TGG 0.493939 8.1:+24831343 MS.gene44431:CDS
AGCCTTGGTCTAGGGTAAGT+TGG 0.505926 8.1:-24831111 None:intergenic
ACCTTTCTATGCTTATTACA+AGG 0.510047 8.1:+24832656 MS.gene44431:CDS
TTAGTGCCATTGAAGCTAGA+AGG 0.511286 8.1:+24832769 MS.gene44431:CDS
CTTACCAGAGCCTTGGTCTA+GGG 0.518702 8.1:-24831119 None:intergenic
ATTATTGTCAAGATAAATCA+AGG 0.521408 8.1:+24832826 MS.gene44431:CDS
ATGGTGATGATTCCAAAGAA+TGG 0.527370 8.1:-24831648 None:intergenic
ACAGTTCCAGCAGAATCTCC+AGG 0.531704 8.1:-24831349 None:intergenic
CTGTATATGGTTGCCCAGTA+CGG 0.532005 8.1:-24831535 None:intergenic
GAATATTTATGCTCATGGAA+AGG 0.532200 8.1:+24831565 MS.gene44431:CDS
TATGTTACTTACCAGAGCCT+TGG 0.535649 8.1:-24831126 None:intergenic
CATATCAAGCAAATTGATCA+AGG 0.537805 8.1:+24831079 MS.gene44431:CDS
CCATTGAAGCTAGAAGGTAT+AGG 0.547043 8.1:+24832775 MS.gene44431:CDS
TGATCAATTTGCTTGATATG+GGG 0.550945 8.1:-24831076 None:intergenic
AGTTCTTGGGAAACCGTACT+GGG 0.556815 8.1:+24831522 MS.gene44431:CDS
ACAAGAACAATGAAGCAAAA+GGG 0.563731 8.1:+24832529 MS.gene44431:CDS
AAAGGGTGATAGAGAACAAA+GGG 0.564292 8.1:+24831583 MS.gene44431:CDS
TCCTTGTAATAAGCATAGAA+AGG 0.566971 8.1:-24832657 None:intergenic
TGACAGTGTTCGTGATGAGT+TGG 0.575375 8.1:+24831493 MS.gene44431:CDS
TAAGTATGAAAGTCAGCAGA+AGG 0.575925 8.1:-24831623 None:intergenic
GATAATTGGGCAACAAGAGG+TGG 0.576591 8.1:+24832606 MS.gene44431:CDS
TTGAAGCTAGAAGGTATAGG+TGG 0.577676 8.1:+24832778 MS.gene44431:CDS
ATGAAGATTAAACTTGTCCC+TGG 0.591788 8.1:+24831331 MS.gene44431:CDS
AGAAGGGTAAAATATGGCCA+TGG 0.594508 8.1:-24830948 None:intergenic
ACCAAACAAGTATTTCACCT+TGG 0.606385 8.1:-24831299 None:intergenic
TAATGTTGAAAATACTCCCA+TGG 0.606737 8.1:-24832574 None:intergenic
GACAATAATCATAGATCACA+TGG 0.610597 8.1:-24832812 None:intergenic
TGCCTGAAAGACACTCATGT+GGG 0.615126 8.1:-24832860 None:intergenic
ATCAAGACTTTCATGTCACG+TGG 0.617056 8.1:+24831047 MS.gene44431:CDS
CTTACCCTAGACCAAGGCTC+TGG 0.621562 8.1:+24831115 MS.gene44431:CDS
GCTGATAATTGGGCAACAAG+AGG 0.638048 8.1:+24832603 MS.gene44431:CDS
CAGTTCCAGCAGAATCTCCA+GGG 0.642838 8.1:-24831348 None:intergenic
ATTCTCGTATCATCTGTTTG+GGG 0.645179 8.1:+24831007 MS.gene44431:CDS
TGAAGCTAGAAGGTATAGGT+GGG 0.659236 8.1:+24832779 MS.gene44431:CDS
GCCTGAAAGACACTCATGTG+GGG 0.659861 8.1:-24832859 None:intergenic
ATGCCTGAAAGACACTCATG+TGG 0.662116 8.1:-24832861 None:intergenic
ATCCAACTTACCCTAGACCA+AGG 0.669978 8.1:+24831109 MS.gene44431:CDS
GAAAGGGTGATAGAGAACAA+AGG 0.672872 8.1:+24831582 MS.gene44431:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATACTATACTAATATAATT+TGG + chr8.1:24831210-24831229 MS.gene44431:intron 10.0%
!! TAAAAGTAAATGAAATGTAT+AGG + chr8.1:24832248-24832267 MS.gene44431:intron 15.0%
!!! AATTTTTTTGTTCAAATAGA+TGG + chr8.1:24831835-24831854 MS.gene44431:intron 15.0%
!!! AGTATTTTTGATAGATAAAT+AGG - chr8.1:24831249-24831268 None:intergenic 15.0%
!!! ATATTTTTACTTACACAATA+TGG - chr8.1:24831670-24831689 None:intergenic 15.0%
!!! TCTTTTTTATGTTGTTATTT+TGG + chr8.1:24830984-24831003 MS.gene44431:CDS 15.0%
!!! TGAATATATTTTTCATTTTG+TGG + chr8.1:24831453-24831472 MS.gene44431:intron 15.0%
!! AAGTGTGACTATAATTAATT+AGG - chr8.1:24832338-24832357 None:intergenic 20.0%
!! AGTTTAGTAGCTATAATTTA+AGG - chr8.1:24832222-24832241 None:intergenic 20.0%
!! ATTATTGTCAAGATAAATCA+AGG + chr8.1:24832826-24832845 MS.gene44431:CDS 20.0%
!! ATTTACCATGAATTTAAATG+TGG - chr8.1:24832107-24832126 None:intergenic 20.0%
!! TTTAGTGAAGAAATTAAAGA+TGG - chr8.1:24832298-24832317 None:intergenic 20.0%
!!! AAATACTAAATTTTTTGCAC+AGG + chr8.1:24831262-24831281 MS.gene44431:intron 20.0%
!!! ACATTTCATTTACTTTTACA+TGG - chr8.1:24832246-24832265 None:intergenic 20.0%
!!! TTTGTTATCTTCACTATATA+TGG + chr8.1:24831858-24831877 MS.gene44431:intron 20.0%
! AAATGTGGTTAACCATAATA+GGG - chr8.1:24832092-24832111 None:intergenic 25.0%
! AATATTTATGCTCATGGAAA+GGG + chr8.1:24831566-24831585 MS.gene44431:CDS 25.0%
! ATATTCGTTTGAACTGTATA+TGG - chr8.1:24831551-24831570 None:intergenic 25.0%
! ATTATGATTCAAACAAAGAG+TGG - chr8.1:24831408-24831427 None:intergenic 25.0%
! GTTAACCACATTTAAATTCA+TGG + chr8.1:24832099-24832118 MS.gene44431:intron 25.0%
! TAAATGTGGTTAACCATAAT+AGG - chr8.1:24832093-24832112 None:intergenic 25.0%
! TTACATAATACATGAAGTGT+TGG + chr8.1:24832166-24832185 MS.gene44431:intron 25.0%
! TTAGTAGCTATAATTTAAGG+TGG - chr8.1:24832219-24832238 None:intergenic 25.0%
! TTGTTCTTGTATACTCTAAT+AGG - chr8.1:24832519-24832538 None:intergenic 25.0%
!! TAATTTAAGGTGGATATATG+AGG - chr8.1:24832209-24832228 None:intergenic 25.0%
!! TACATAATACATGAAGTGTT+GGG + chr8.1:24832167-24832186 MS.gene44431:intron 25.0%
!! TTGATCAATTTGCTTGATAT+GGG - chr8.1:24831080-24831099 None:intergenic 25.0%
!!! GTGGATTAGAGAAAATTAAT+TGG + chr8.1:24832625-24832644 MS.gene44431:CDS 25.0%
!!! GTTTTTAGAAGGGTAAAATA+TGG - chr8.1:24830957-24830976 None:intergenic 25.0%
!!! TACAAGGATTTTGATATTGA+AGG + chr8.1:24832672-24832691 MS.gene44431:CDS 25.0%
!!! TATCATATATTTTGGACATC+TGG - chr8.1:24831169-24831188 None:intergenic 25.0%
!!! TTTTACCCTTCTAAAAACAA+TGG + chr8.1:24830959-24830978 MS.gene44431:CDS 25.0%
AAGTCTTGATTAAAAGTTGC+TGG - chr8.1:24831037-24831056 None:intergenic 30.0%
ACAAGAACAATGAAGCAAAA+GGG + chr8.1:24832529-24832548 MS.gene44431:CDS 30.0%
ACCTTTCTATGCTTATTACA+AGG + chr8.1:24832656-24832675 MS.gene44431:CDS 30.0%
ATTATGGGAAGCTGATAATT+GGG + chr8.1:24832593-24832612 MS.gene44431:CDS 30.0%
CAAACGAATATTTATGCTCA+TGG + chr8.1:24831560-24831579 MS.gene44431:CDS 30.0%
CATATCAAGCAAATTGATCA+AGG + chr8.1:24831079-24831098 MS.gene44431:CDS 30.0%
CCTAATAACACAAGATAAGA+AGG + chr8.1:24831955-24831974 MS.gene44431:intron 30.0%
GAAATTAAAGATGGTTGGAT+TGG - chr8.1:24832289-24832308 None:intergenic 30.0%
GAATATTTATGCTCATGGAA+AGG + chr8.1:24831565-24831584 MS.gene44431:CDS 30.0%
GACAATAATCATAGATCACA+TGG - chr8.1:24832815-24832834 None:intergenic 30.0%
GTGAAGAAATTAAAGATGGT+TGG - chr8.1:24832294-24832313 None:intergenic 30.0%
TAATGTTGAAAATACTCCCA+TGG - chr8.1:24832577-24832596 None:intergenic 30.0%
TACAAGAACAATGAAGCAAA+AGG + chr8.1:24832528-24832547 MS.gene44431:CDS 30.0%
TCCTTGTAATAAGCATAGAA+AGG - chr8.1:24832660-24832679 None:intergenic 30.0%
TTCATACTTACTCCATTCTT+TGG + chr8.1:24831636-24831655 MS.gene44431:CDS 30.0%
! CCTTCTTATCTTGTGTTATT+AGG - chr8.1:24831958-24831977 None:intergenic 30.0%
! CTTGATCAATTTGCTTGATA+TGG - chr8.1:24831081-24831100 None:intergenic 30.0%
! TGATCAATTTGCTTGATATG+GGG - chr8.1:24831079-24831098 None:intergenic 30.0%
!!! AAATTTTTTGCACAGGTTGT+GGG + chr8.1:24831269-24831288 MS.gene44431:intron 30.0%
!!! AAGACACCATTGTTTTTAGA+AGG - chr8.1:24830968-24830987 None:intergenic 30.0%
!!! AGACACCATTGTTTTTAGAA+GGG - chr8.1:24830967-24830986 None:intergenic 30.0%
!!! GGGAGTATTTTCAACATTAT+GGG + chr8.1:24832578-24832597 MS.gene44431:CDS 30.0%
!!! TAAATTTTTTGCACAGGTTG+TGG + chr8.1:24831268-24831287 MS.gene44431:intron 30.0%
!!! TGGGAGTATTTTCAACATTA+TGG + chr8.1:24832577-24832596 MS.gene44431:CDS 30.0%
AAAACCTTGAATACACATGC+AGG + chr8.1:24832475-24832494 MS.gene44431:intron 35.0%
AAAGGGTGATAGAGAACAAA+GGG + chr8.1:24831583-24831602 MS.gene44431:CDS 35.0%
AAGTTTGCATGTCCCTATTA+TGG + chr8.1:24832077-24832096 MS.gene44431:intron 35.0%
ACATGCAAACTTGTACCTTT+GGG - chr8.1:24832069-24832088 None:intergenic 35.0%
ACCAAACAAGTATTTCACCT+TGG - chr8.1:24831302-24831321 None:intergenic 35.0%
ATGAAGATTAAACTTGTCCC+TGG + chr8.1:24831331-24831350 MS.gene44431:CDS 35.0%
ATTCTCGTATCATCTGTTTG+GGG + chr8.1:24831007-24831026 MS.gene44431:CDS 35.0%
CACTAACCCAAAAAATTGGT+GGG + chr8.1:24832725-24832744 MS.gene44431:CDS 35.0%
CATTATGGGAAGCTGATAAT+TGG + chr8.1:24832592-24832611 MS.gene44431:CDS 35.0%
GAGAACAAAGGGTTAATCTT+TGG + chr8.1:24831594-24831613 MS.gene44431:CDS 35.0%
GATTCTCGTATCATCTGTTT+GGG + chr8.1:24831006-24831025 MS.gene44431:CDS 35.0%
TAAGTATGAAAGTCAGCAGA+AGG - chr8.1:24831626-24831645 None:intergenic 35.0%
TCCAAGGTGAAATACTTGTT+TGG + chr8.1:24831298-24831317 MS.gene44431:CDS 35.0%
TGATGTTGAAAATACTCCCA+TGG - chr8.1:24832393-24832412 None:intergenic 35.0%
! ATGGTGATGATTCCAAAGAA+TGG - chr8.1:24831651-24831670 None:intergenic 35.0%
! CATCATGGGAAGTTTTCAAA+TGG + chr8.1:24832408-24832427 MS.gene44431:intron 35.0%
!!! GAGTTGGATTTTGAGTTCTT+GGG + chr8.1:24831509-24831528 MS.gene44431:CDS 35.0%
!!! GGGAGTATTTTCAACATCAT+GGG + chr8.1:24832394-24832413 MS.gene44431:intron 35.0%
!!! TGAGTTGGATTTTGAGTTCT+TGG + chr8.1:24831508-24831527 MS.gene44431:CDS 35.0%
!!! TGGGAGTATTTTCAACATCA+TGG + chr8.1:24832393-24832412 MS.gene44431:intron 35.0%
ACCCAAAAAATTGGTGGGAA+AGG + chr8.1:24832730-24832749 MS.gene44431:CDS 40.0%
AGAACCTGCATGTGTATTCA+AGG - chr8.1:24832482-24832501 None:intergenic 40.0%
ATACCCAAAAATGCAAGCCA+TGG + chr8.1:24832557-24832576 MS.gene44431:CDS 40.0%
ATCAAGACTTTCATGTCACG+TGG + chr8.1:24831047-24831066 MS.gene44431:CDS 40.0%
ATCGTAAAAGATGCAAGCCA+TGG + chr8.1:24832373-24832392 MS.gene44431:intron 40.0%
ATTGAAGGATGTGCAATTCC+AGG + chr8.1:24832687-24832706 MS.gene44431:CDS 40.0%
CATGCAAACTTGTACCTTTG+GGG - chr8.1:24832068-24832087 None:intergenic 40.0%
CCACTAACCCAAAAAATTGG+TGG + chr8.1:24832724-24832743 MS.gene44431:CDS 40.0%
CCCAAAAAATTGGTGGGAAA+GGG + chr8.1:24832731-24832750 MS.gene44431:CDS 40.0%
GAAAGGGTGATAGAGAACAA+AGG + chr8.1:24831582-24831601 MS.gene44431:CDS 40.0%
GACATGCAAACTTGTACCTT+TGG - chr8.1:24832070-24832089 None:intergenic 40.0%
GGATTCTCGTATCATCTGTT+TGG + chr8.1:24831005-24831024 MS.gene44431:CDS 40.0%
TACCCAAAAATGCAAGCCAT+GGG + chr8.1:24832558-24832577 MS.gene44431:CDS 40.0%
TCGTAAAAGATGCAAGCCAT+GGG + chr8.1:24832374-24832393 MS.gene44431:intron 40.0%
! CCATTGAAGCTAGAAGGTAT+AGG + chr8.1:24832775-24832794 MS.gene44431:CDS 40.0%
! GTGCCACTAACCCAAAAAAT+TGG + chr8.1:24832721-24832740 MS.gene44431:CDS 40.0%
! TATGTTACTTACCAGAGCCT+TGG - chr8.1:24831129-24831148 None:intergenic 40.0%
! TGAAGCTAGAAGGTATAGGT+GGG + chr8.1:24832779-24832798 MS.gene44431:CDS 40.0%
! TTAGTGCCATTGAAGCTAGA+AGG + chr8.1:24832769-24832788 MS.gene44431:CDS 40.0%
! TTGAAGCTAGAAGGTATAGG+TGG + chr8.1:24832778-24832797 MS.gene44431:CDS 40.0%
!! CCTATACCTTCTAGCTTCAA+TGG - chr8.1:24832778-24832797 None:intergenic 40.0%
!! TACCTTTGGGGTACAAGTTA+AGG - chr8.1:24832056-24832075 None:intergenic 40.0%
!!! ACCCTTTCCCACCAATTTTT+TGG - chr8.1:24832735-24832754 None:intergenic 40.0%
!!! ATGGCTTGCATTTTTGGGTA+TGG - chr8.1:24832558-24832577 None:intergenic 40.0%
!!! CCACCAATTTTTTGGGTTAG+TGG - chr8.1:24832727-24832746 None:intergenic 40.0%
!!! CCCTTTCCCACCAATTTTTT+GGG - chr8.1:24832734-24832753 None:intergenic 40.0%
AGTTCTTGGGAAACCGTACT+GGG + chr8.1:24831522-24831541 MS.gene44431:CDS 45.0%
ATCCAACTTACCCTAGACCA+AGG + chr8.1:24831109-24831128 MS.gene44431:CDS 45.0%
ATGCCTGAAAGACACTCATG+TGG - chr8.1:24832864-24832883 None:intergenic 45.0%
CTGTATATGGTTGCCCAGTA+CGG - chr8.1:24831538-24831557 None:intergenic 45.0%
GATAATTGGGCAACAAGAGG+TGG + chr8.1:24832606-24832625 MS.gene44431:CDS 45.0%
GCTGATAATTGGGCAACAAG+AGG + chr8.1:24832603-24832622 MS.gene44431:CDS 45.0%
TGACAGTGTTCGTGATGAGT+TGG + chr8.1:24831493-24831512 MS.gene44431:CDS 45.0%
TGCCTGAAAGACACTCATGT+GGG - chr8.1:24832863-24832882 None:intergenic 45.0%
!! ACTCCCATGGCTTGCATTTT+TGG - chr8.1:24832564-24832583 None:intergenic 45.0%
!! CTCCCATGGCTTGCATTTTT+GGG - chr8.1:24832563-24832582 None:intergenic 45.0%
!! GTCCTTAACTTGTACCCCAA+AGG + chr8.1:24832051-24832070 MS.gene44431:intron 45.0%
!!! TAATAAAATATCATATATTT+TGG - chr8.1:24831177-24831196 None:intergenic 5.0%
ACAGTTCCAGCAGAATCTCC+AGG - chr8.1:24831352-24831371 None:intergenic 50.0%
ACCCCACATGAGTGTCTTTC+AGG + chr8.1:24832858-24832877 MS.gene44431:CDS 50.0%
ACTTACCAGAGCCTTGGTCT+AGG - chr8.1:24831123-24831142 None:intergenic 50.0%
AGACACTCATGTGGGGTCAT+TGG - chr8.1:24832855-24832874 None:intergenic 50.0%
CAGTTCCAGCAGAATCTCCA+GGG - chr8.1:24831351-24831370 None:intergenic 50.0%
CTTACCAGAGCCTTGGTCTA+GGG - chr8.1:24831122-24831141 None:intergenic 50.0%
GACACTCATGTGGGGTCATT+GGG - chr8.1:24832854-24832873 None:intergenic 50.0%
GAGTTCTTGGGAAACCGTAC+TGG + chr8.1:24831521-24831540 MS.gene44431:CDS 50.0%
GCCTGAAAGACACTCATGTG+GGG - chr8.1:24832862-24832881 None:intergenic 50.0%
! AGCCTTGGTCTAGGGTAAGT+TGG - chr8.1:24831114-24831133 None:intergenic 50.0%
! AGGTTGTGGGTTTGCTTCCA+AGG + chr8.1:24831282-24831301 MS.gene44431:intron 50.0%
! GGGTTAGTGGCACATGTAGT+TGG - chr8.1:24832714-24832733 None:intergenic 50.0%
CTTACCCTAGACCAAGGCTC+TGG + chr8.1:24831115-24831134 MS.gene44431:CDS 55.0%
CTTGTCCCTGGAGATTCTGC+TGG + chr8.1:24831343-24831362 MS.gene44431:CDS 55.0%
GTGGCACATGTAGTTGGTCC+TGG - chr8.1:24832708-24832727 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 24830950 24832887 24830950 ID=MS.gene44431
chr8.1 mRNA 24830950 24832887 24830950 ID=MS.gene44431.t1;Parent=MS.gene44431
chr8.1 exon 24830950 24831136 24830950 ID=MS.gene44431.t1.exon1;Parent=MS.gene44431.t1
chr8.1 CDS 24830950 24831136 24830950 ID=cds.MS.gene44431.t1;Parent=MS.gene44431.t1
chr8.1 exon 24831284 24831384 24831284 ID=MS.gene44431.t1.exon2;Parent=MS.gene44431.t1
chr8.1 CDS 24831284 24831384 24831284 ID=cds.MS.gene44431.t1;Parent=MS.gene44431.t1
chr8.1 exon 24831479 24831675 24831479 ID=MS.gene44431.t1.exon3;Parent=MS.gene44431.t1
chr8.1 CDS 24831479 24831675 24831479 ID=cds.MS.gene44431.t1;Parent=MS.gene44431.t1
chr8.1 exon 24832497 24832887 24832497 ID=MS.gene44431.t1.exon4;Parent=MS.gene44431.t1
chr8.1 CDS 24832497 24832887 24832497 ID=cds.MS.gene44431.t1;Parent=MS.gene44431.t1
Gene Sequence

>MS.gene44431

ATGGCCATATTTTACCCTTCTAAAAACAATGGTGTCTTTTTTATGTTGTTATTTTGGATTCTCGTATCATCTGTTTGGGGAAGACCAGCAACTTTTAATCAAGACTTTCATGTCACGTGGTCTGAACCCCATATCAAGCAAATTGATCAAGGCAGAACTATCCAACTTACCCTAGACCAAGGCTCTGGTAAGTAACATATGTTATTATATACATGTCCAGATGTCCAAAATATATGATATTTTATTAAACATAAATAACAAATACTATACTAATATAATTTGGATAAGTCATGTTACCTATTTATCTATCAAAAATACTAAATTTTTTGCACAGGTTGTGGGTTTGCTTCCAAGGTGAAATACTTGTTTGGTCGTGTTAGCATGAAGATTAAACTTGTCCCTGGAGATTCTGCTGGAACTGTTACTGCATTTTATGTAAGTTCAAATTAATTTCTCCACTCTTTGTTTGAATCATAATTAAGTGAGACAAAAACTGAAATCACTGAATATATTTTTCATTTTGTGGTAGATGAACTCTGACACTGACAGTGTTCGTGATGAGTTGGATTTTGAGTTCTTGGGAAACCGTACTGGGCAACCATATACAGTTCAAACGAATATTTATGCTCATGGAAAGGGTGATAGAGAACAAAGGGTTAATCTTTGGTTTGATCCTTCTGCTGACTTTCATACTTACTCCATTCTTTGGAATCATCACCATATTGTGTAAGTAAAAATATATATAAGCATAATGATGTGATTCTGTTAATAAAAATAAATATAATTGCATAATGTAAATCACAAAGTTGCATTGAGATATTTGATATTTCAGTTGTGTTATTTGAACAATCAAATTTAGAAACTATTTTAGAGAACGTTTTAGACAATTTTTTTGTTCAAATAGATGGTTTGTTATCTTCACTATATATGGTTCAAATTATGTATTTTTTTTTTAAGCAAAATCACATAGTTGAACACTTAAATTATGAAAATGTTAACTTGTATCCTAATAACACAAGATAAGAAGGAAACGTAAAAGTGTAATATTGAAAATTGTGTATTTATTATTTTTTAAATGCAACATTTTTTTTTCATTTTTAAGTCCTTAACTTGTACCCCAAAGGTACAAGTTTGCATGTCCCTATTATGGTTAACCACATTTAAATTCATGGTAAATGAAGTTAAATTGATCATTAATGAGTCTCAACATCTATGATTACATAATACATGAAGTGTTGGGTTTTGAACTATGTACACCTCATATATCCACCTTAAATTATAGCTACTAAACTACCATGTAAAAGTAAATGAAATGTATAGGAGAAACATGTAACATCCAATCCAACCATCTTTAATTTCTTCACTAAATTTAGCAAATATTTTGACCTAATTAATTATAGTCACACTTTTGTCACAAAATCATATCGTAAAAGATGCAAGCCATGGGAGTATTTTCAACATCATGGGAAGTTTTCAAATGGTCATGAATGTAATTTTGATTAATCATAGCTTCTATGATTTATTTAAAACCTTGAATACACATGCAGGTTCTACGTTGATGAAGTTCCTATTAGAGTATACAAGAACAATGAAGCAAAAGGGATACCATACCCAAAAATGCAAGCCATGGGAGTATTTTCAACATTATGGGAAGCTGATAATTGGGCAACAAGAGGTGGATTAGAGAAAATTAATTGGAGTAAAGCACCTTTCTATGCTTATTACAAGGATTTTGATATTGAAGGATGTGCAATTCCAGGACCAACTACATGTGCCACTAACCCAAAAAATTGGTGGGAAAGGGTTGAATATCAAGCTCTTAGTGCCATTGAAGCTAGAAGGTATAGGTGGGTTCGTATGAACCATGTGATCTATGATTATTGTCAAGATAAATCAAGGTACCCAATGACCCCACATGAGTGTCTTTCAGGCATTTAA
Protein sequence

>MS.gene44431.t1

MAIFYPSKNNGVFFMLLFWILVSSVWGRPATFNQDFHVTWSEPHIKQIDQGRTIQLTLDQGSGCGFASKVKYLFGRVSMKIKLVPGDSAGTVTAFYMNSDTDSVRDELDFEFLGNRTGQPYTVQTNIYAHGKGDREQRVNLWFDPSADFHTYSILWNHHHIVFYVDEVPIRVYKNNEAKGIPYPKMQAMGVFSTLWEADNWATRGGLEKINWSKAPFYAYYKDFDIEGCAIPGPTTCATNPKNWWERVEYQALSAIEARRYRWVRMNHVIYDYCQDKSRYPMTPHECLSGI