Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69213.t1 | XP_013457984.1 | 97.7 | 87 | 2 | 0 | 1 | 87 | 22 | 108 | 1.30E-43 | 185.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69213.t1 | Q9SD84 | 65.6 | 90 | 30 | 1 | 1 | 89 | 19 | 108 | 2.8e-31 | 135.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69213.t1 | I3SNP7 | 97.7 | 87 | 2 | 0 | 1 | 87 | 22 | 108 | 9.3e-44 | 185.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052308 | MS.gene69213 | 0.816045 | 6.77E-52 | -1.69E-46 |
| MS.gene053348 | MS.gene69213 | 0.813455 | 2.54E-51 | -1.69E-46 |
| MS.gene054551 | MS.gene69213 | 0.803011 | 4.27E-49 | -1.69E-46 |
| MS.gene055308 | MS.gene69213 | 0.827763 | 1.31E-54 | -1.69E-46 |
| MS.gene055578 | MS.gene69213 | 0.814399 | 1.57E-51 | -1.69E-46 |
| MS.gene055957 | MS.gene69213 | 0.84556 | 3.76E-59 | -1.69E-46 |
| MS.gene056041 | MS.gene69213 | 0.844069 | 9.50E-59 | -1.69E-46 |
| MS.gene056042 | MS.gene69213 | 0.826493 | 2.63E-54 | -1.69E-46 |
| MS.gene05640 | MS.gene69213 | 0.804702 | 1.90E-49 | -1.69E-46 |
| MS.gene05652 | MS.gene69213 | 0.819386 | 1.19E-52 | -1.69E-46 |
| MS.gene057458 | MS.gene69213 | 0.801282 | 9.69E-49 | -1.69E-46 |
| MS.gene058128 | MS.gene69213 | 0.823867 | 1.10E-53 | -1.69E-46 |
| MS.gene058853 | MS.gene69213 | 0.807698 | 4.45E-50 | -1.69E-46 |
| MS.gene060473 | MS.gene69213 | 0.801369 | 9.30E-49 | -1.69E-46 |
| MS.gene060825 | MS.gene69213 | 0.813857 | 2.07E-51 | -1.69E-46 |
| MS.gene06093 | MS.gene69213 | 0.810688 | 1.02E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATGAGCTCTAACGGTGTT+TGG | 0.235083 | 8.2:-16444963 | None:intergenic |
| GTGCTTGTTGACCCTTCTTC+TGG | 0.287043 | 8.2:-16445010 | None:intergenic |
| TGACATATGTAGGTGCTAAT+TGG | 0.376958 | 8.2:+16444832 | None:intergenic |
| TTGGACTATGCTTGTGGAGC+TGG | 0.394023 | 8.2:+16444897 | MS.gene69213:CDS |
| AATGAGCTCTAACGGTGTTT+GGG | 0.405145 | 8.2:-16444962 | None:intergenic |
| AATTTAAACCTGGCACTTGA+AGG | 0.429774 | 8.2:-16445204 | None:intergenic |
| GCACTGGCTTGTGACTTTGC+AGG | 0.430148 | 8.2:+16445029 | MS.gene69213:CDS |
| TCTTAATGAGCAGGTGTTAG+TGG | 0.445255 | 8.2:+16445171 | MS.gene69213:intron |
| GTGCCTACCCTTCAAGTGCC+AGG | 0.455839 | 8.2:+16445196 | MS.gene69213:CDS |
| GAATAGCTATTACCAGAAGA+AGG | 0.510643 | 8.2:+16444998 | MS.gene69213:CDS |
| TGCTAATTGGTGCGTGTGCA+AGG | 0.528841 | 8.2:+16444845 | MS.gene69213:CDS |
| AATTGGTGCGTGTGCAAGGA+TGG | 0.547293 | 8.2:+16444849 | MS.gene69213:CDS |
| ATTTAAACCTGGCACTTGAA+GGG | 0.550247 | 8.2:-16445203 | None:intergenic |
| TGCAATCTTACAAAAGACCT+TGG | 0.568888 | 8.2:+16444878 | MS.gene69213:CDS |
| GAAGAAGGGTCAACAAGCAC+TGG | 0.571335 | 8.2:+16445013 | MS.gene69213:CDS |
| ACGGTGTTTGGGTTGTAACA+AGG | 0.576603 | 8.2:-16444951 | None:intergenic |
| AAACCTGGCACTTGAAGGGT+AGG | 0.578796 | 8.2:-16445199 | None:intergenic |
| ATAGCTGCAATGAGCTCTAA+CGG | 0.591747 | 8.2:-16444970 | None:intergenic |
| AATAGCTATTACCAGAAGAA+GGG | 0.608399 | 8.2:+16444999 | MS.gene69213:CDS |
| AAGACCTTGGACTATGCTTG+TGG | 0.615068 | 8.2:+16444891 | MS.gene69213:CDS |
| AGCTCCACAAGCATAGTCCA+AGG | 0.629352 | 8.2:-16444895 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TACTTTTGTTTATCATAATA+GGG | + | chr8.2:16445341-16445360 | MS.gene69213:intron | 15.0% |
| !!! | TTACTTTTGTTTATCATAAT+AGG | + | chr8.2:16445340-16445359 | MS.gene69213:intron | 15.0% |
| !!! | TTAGCTACAATTATTTTAAA+AGG | - | chr8.2:16445120-16445139 | None:intergenic | 15.0% |
| !! | TAGAGAAAAAAATTTAAACC+TGG | - | chr8.2:16445217-16445236 | None:intergenic | 20.0% |
| ! | AAGGAAAAACAAGAAAGAAA+AGG | - | chr8.2:16445101-16445120 | None:intergenic | 25.0% |
| !! | TTGTAGCTAACTCTTTTTAT+TGG | + | chr8.2:16445130-16445149 | MS.gene69213:intron | 25.0% |
| !!! | TGTGTTTTTGAAGAATCTTA+TGG | + | chr8.2:16445297-16445316 | MS.gene69213:intron | 25.0% |
| AAGAATCTTATGGACAATAG+TGG | + | chr8.2:16445307-16445326 | MS.gene69213:intron | 30.0% | |
| AATAGCTATTACCAGAAGAA+GGG | + | chr8.2:16444999-16445018 | MS.gene69213:CDS | 30.0% | |
| GGTGAAAATGAGTATTTACT+TGG | - | chr8.2:16445080-16445099 | None:intergenic | 30.0% | |
| AATTTAAACCTGGCACTTGA+AGG | - | chr8.2:16445207-16445226 | None:intergenic | 35.0% | |
| ATTTAAACCTGGCACTTGAA+GGG | - | chr8.2:16445206-16445225 | None:intergenic | 35.0% | |
| GAATAGCTATTACCAGAAGA+AGG | + | chr8.2:16444998-16445017 | MS.gene69213:CDS | 35.0% | |
| TCTGTTTGTTCTTAATGAGC+AGG | + | chr8.2:16445162-16445181 | MS.gene69213:intron | 35.0% | |
| TGCAATCTTACAAAAGACCT+TGG | + | chr8.2:16444878-16444897 | MS.gene69213:CDS | 35.0% | |
| TCTTAATGAGCAGGTGTTAG+TGG | + | chr8.2:16445171-16445190 | MS.gene69213:intron | 40.0% | |
| ! | AATGAGCTCTAACGGTGTTT+GGG | - | chr8.2:16444965-16444984 | None:intergenic | 40.0% |
| ! | ATAGCTGCAATGAGCTCTAA+CGG | - | chr8.2:16444973-16444992 | None:intergenic | 40.0% |
| AAGACCTTGGACTATGCTTG+TGG | + | chr8.2:16444891-16444910 | MS.gene69213:CDS | 45.0% | |
| ! | CAATGAGCTCTAACGGTGTT+TGG | - | chr8.2:16444966-16444985 | None:intergenic | 45.0% |
| !! | ACGGTGTTTGGGTTGTAACA+AGG | - | chr8.2:16444954-16444973 | None:intergenic | 45.0% |
| !!! | AAGGAGCGTTTTGACGAAGA+GGG | - | chr8.2:16444935-16444954 | None:intergenic | 45.0% |
| AAACCTGGCACTTGAAGGGT+AGG | - | chr8.2:16445202-16445221 | None:intergenic | 50.0% | |
| AATTGGTGCGTGTGCAAGGA+TGG | + | chr8.2:16444849-16444868 | MS.gene69213:CDS | 50.0% | |
| AGCTCCACAAGCATAGTCCA+AGG | - | chr8.2:16444898-16444917 | None:intergenic | 50.0% | |
| GAAGAAGGGTCAACAAGCAC+TGG | + | chr8.2:16445013-16445032 | MS.gene69213:CDS | 50.0% | |
| TGCTAATTGGTGCGTGTGCA+AGG | + | chr8.2:16444845-16444864 | MS.gene69213:CDS | 50.0% | |
| TTGGACTATGCTTGTGGAGC+TGG | + | chr8.2:16444897-16444916 | MS.gene69213:CDS | 50.0% | |
| ! | GTGCTTGTTGACCCTTCTTC+TGG | - | chr8.2:16445013-16445032 | None:intergenic | 50.0% |
| !!! | CAAGGAGCGTTTTGACGAAG+AGG | - | chr8.2:16444936-16444955 | None:intergenic | 50.0% |
| !! | GCACTGGCTTGTGACTTTGC+AGG | + | chr8.2:16445029-16445048 | MS.gene69213:CDS | 55.0% |
| GTGCCTACCCTTCAAGTGCC+AGG | + | chr8.2:16445196-16445215 | MS.gene69213:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 16444844 | 16445368 | 16444844 | ID=MS.gene69213 |
| chr8.2 | mRNA | 16444844 | 16445368 | 16444844 | ID=MS.gene69213.t1;Parent=MS.gene69213 |
| chr8.2 | exon | 16444844 | 16445080 | 16444844 | ID=MS.gene69213.t1.exon1;Parent=MS.gene69213.t1 |
| chr8.2 | CDS | 16444844 | 16445080 | 16444844 | ID=cds.MS.gene69213.t1;Parent=MS.gene69213.t1 |
| chr8.2 | exon | 16445184 | 16445217 | 16445184 | ID=MS.gene69213.t1.exon2;Parent=MS.gene69213.t1 |
| chr8.2 | CDS | 16445184 | 16445217 | 16445184 | ID=cds.MS.gene69213.t1;Parent=MS.gene69213.t1 |
| chr8.2 | exon | 16445362 | 16445368 | 16445362 | ID=MS.gene69213.t1.exon3;Parent=MS.gene69213.t1 |
| chr8.2 | CDS | 16445362 | 16445368 | 16445362 | ID=cds.MS.gene69213.t1;Parent=MS.gene69213.t1 |
| Gene Sequence |
| Protein sequence |
