Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69410.t1 | XP_003603744.1 | 99.2 | 253 | 2 | 0 | 1 | 253 | 1 | 253 | 3.10E-139 | 505 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69410.t1 | O81769 | 79.9 | 254 | 50 | 1 | 1 | 253 | 1 | 254 | 2.4e-116 | 420.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69410.t1 | G7J4Q4 | 99.2 | 253 | 2 | 0 | 1 | 253 | 1 | 253 | 2.2e-139 | 505.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene69410 | MS.gene83565 | PPI |
| MS.gene69410 | MS.gene067444 | PPI |
| MS.gene69410 | MS.gene85931 | PPI |
| MS.gene69410 | MS.gene86968 | PPI |
| MS.gene69410 | MS.gene009798 | PPI |
| MS.gene50211 | MS.gene69410 | PPI |
| MS.gene69410 | MS.gene56983 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69410.t1 | MTR_3g113750 | 99.209 | 253 | 2 | 0 | 1 | 253 | 1 | 253 | 0.0 | 518 |
| MS.gene69410.t1 | MTR_1g107520 | 87.879 | 165 | 16 | 1 | 1 | 165 | 1 | 161 | 6.84e-101 | 296 |
| MS.gene69410.t1 | MTR_3g114210 | 74.324 | 148 | 29 | 3 | 31 | 169 | 1 | 148 | 1.67e-63 | 200 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69410.t1 | AT4G31790 | 79.921 | 254 | 50 | 1 | 1 | 253 | 1 | 254 | 1.58e-152 | 431 |
| MS.gene69410.t1 | AT4G31790 | 79.921 | 254 | 50 | 1 | 1 | 253 | 1 | 254 | 1.58e-152 | 431 |
Find 83 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTCAACAAAATCGAAATT+TGG | 0.119650 | 3.3:-93141878 | MS.gene69410:CDS |
| TTTGCCAATGTAGTTCATTT+TGG | 0.131895 | 3.3:-93138876 | MS.gene69410:CDS |
| CACAGCCATTGAGCAGTTAT+TGG | 0.191629 | 3.3:-93141176 | MS.gene69410:CDS |
| CTTCACACACTTTGCTTGTT+AGG | 0.206959 | 3.3:-93141853 | MS.gene69410:intron |
| GTGTGTTGGGCTTGCTCGTC+TGG | 0.214070 | 3.3:-93140215 | MS.gene69410:CDS |
| TTGATATGGTTACAGATATT+CGG | 0.268916 | 3.3:-93141479 | MS.gene69410:intron |
| CATGCTGCTTTCCTTGTTGT+TGG | 0.296764 | 3.3:-93142192 | MS.gene69410:CDS |
| ACAAAGTTGATCATGGATTC+TGG | 0.311647 | 3.3:+93133112 | None:intergenic |
| CTTGTTGTTGGTGATCCTTT+TGG | 0.312531 | 3.3:-93142180 | MS.gene69410:CDS |
| GATGTAGAAGGTTTATCTTT+AGG | 0.325903 | 3.3:-93133167 | MS.gene69410:CDS |
| ATGTTATACATAATCGGTTT+AGG | 0.334885 | 3.3:-93142432 | MS.gene69410:CDS |
| ATTCAACAAAATCGAAATTT+GGG | 0.350651 | 3.3:-93141877 | MS.gene69410:CDS |
| TGTGTTTATAGTCATATATC+TGG | 0.356508 | 3.3:+93141195 | None:intergenic |
| TACATAATCGGTTTAGGCTT+GGG | 0.356873 | 3.3:-93142426 | MS.gene69410:CDS |
| CAACTACAATTGATTGACTT+TGG | 0.358077 | 3.3:-93140154 | MS.gene69410:CDS |
| TGATATGGTTACAGATATTC+GGG | 0.366444 | 3.3:-93141478 | MS.gene69410:intron |
| TACACTTCCCTTCTCTCCTT+CGG | 0.369995 | 3.3:-93142333 | MS.gene69410:CDS |
| GAATCATAGGATCAACGTTT+TGG | 0.371486 | 3.3:-93138838 | MS.gene69410:intron |
| TTAGGAAGTGGTACAACGTT+TGG | 0.386662 | 3.3:-93133197 | MS.gene69410:CDS |
| TCTCTGTCAGCAAGAGTAAT+GGG | 0.388366 | 3.3:+93142264 | None:intergenic |
| TTTGGAGGTTGTGATGTAGA+AGG | 0.409971 | 3.3:-93133179 | MS.gene69410:CDS |
| TACTCTTGCTGACAGAGAAA+TGG | 0.420268 | 3.3:-93142259 | MS.gene69410:CDS |
| ACTCTGCAGCAGGGAATCAT+AGG | 0.431491 | 3.3:-93138851 | MS.gene69410:CDS |
| GCATCTGTGATGAATGCAAT+TGG | 0.434280 | 3.3:-93141991 | MS.gene69410:CDS |
| GATGGTGAATCCAGCTCTAC+AGG | 0.439112 | 3.3:+93133239 | None:intergenic |
| AGCATGAGAAAGTTGAGCTT+GGG | 0.452656 | 3.3:+93142209 | None:intergenic |
| TCACAAGCACGTGAAGAATC+TGG | 0.453700 | 3.3:-93141148 | MS.gene69410:intron |
| GAGACATCATCCTCTAGATT+AGG | 0.458119 | 3.3:-93133215 | MS.gene69410:CDS |
| GGTTTGCAGCTATATCGTTA+TGG | 0.461327 | 3.3:-93141961 | MS.gene69410:CDS |
| CTCATCACTACCTGTAGAGC+TGG | 0.466699 | 3.3:-93133249 | MS.gene69410:CDS |
| CAGAAGATACCGAGTGTGTT+GGG | 0.467905 | 3.3:-93140228 | MS.gene69410:CDS |
| CAATTCCCATCTTCTTAGCT+CGG | 0.470476 | 3.3:+93142034 | None:intergenic |
| TAAGCTTTAGTATGAGAATG+AGG | 0.472179 | 3.3:-93138928 | MS.gene69410:intron |
| ATACATAATCGGTTTAGGCT+TGG | 0.473388 | 3.3:-93142427 | MS.gene69410:CDS |
| GAAGATCAAATAATAGTAGC+TGG | 0.474742 | 3.3:-93140187 | MS.gene69410:CDS |
| CGACACTTGAATCTTTGTGC+AGG | 0.475224 | 3.3:-93141446 | MS.gene69410:intron |
| ATGCATTTGCCGAAATTACC+TGG | 0.477905 | 3.3:-93133081 | MS.gene69410:CDS |
| TTCTCTGTCAGCAAGAGTAA+TGG | 0.482945 | 3.3:+93142263 | None:intergenic |
| CAGCATGAGAAAGTTGAGCT+TGG | 0.490714 | 3.3:+93142208 | None:intergenic |
| AAGAGAGGCCGAAGGAGAGA+AGG | 0.500937 | 3.3:+93142325 | None:intergenic |
| GAATGGTACAAAGTTGATCA+TGG | 0.508346 | 3.3:+93133105 | None:intergenic |
| TGTGTTGGGCTTGCTCGTCT+GGG | 0.512649 | 3.3:-93140214 | MS.gene69410:CDS |
| GGAAGTGGTACAACGTTTGG+AGG | 0.514419 | 3.3:-93133194 | MS.gene69410:CDS |
| AGAGTAGGTGCGTACCCAAA+AGG | 0.522237 | 3.3:+93142165 | None:intergenic |
| AAATTACCTGGCACAAAATG+AGG | 0.524815 | 3.3:-93133069 | MS.gene69410:CDS |
| ATATGGAAGAAGAAAATCAC+TGG | 0.530962 | 3.3:+93133043 | None:intergenic |
| AGGTAATTTCGGCAAATGCA+TGG | 0.547265 | 3.3:+93133083 | None:intergenic |
| CATTGGCAAAGGTGAGGATG+TGG | 0.549089 | 3.3:+93138889 | None:intergenic |
| ACAGAAGATACCGAGTGTGT+TGG | 0.558228 | 3.3:-93140229 | MS.gene69410:CDS |
| TGTTGTCCGAGCTAAGAAGA+TGG | 0.559050 | 3.3:-93142040 | MS.gene69410:CDS |
| TGGAGAGGCCGTGTGAAGAG+AGG | 0.560627 | 3.3:+93142310 | None:intergenic |
| GTTCCCAAAATGAACTACAT+TGG | 0.562415 | 3.3:+93138872 | None:intergenic |
| AAAATGCGACAAGGTTTACA+TGG | 0.566134 | 3.3:-93142361 | MS.gene69410:CDS |
| AGAGAGGCCGAAGGAGAGAA+GGG | 0.567567 | 3.3:+93142326 | None:intergenic |
| GTGTTTATAGTCATATATCT+GGG | 0.568517 | 3.3:+93141196 | None:intergenic |
| TACCTTTCTTTACTGAGACG+TGG | 0.569946 | 3.3:-93141924 | MS.gene69410:CDS |
| AATCTCCAATAACTGCTCAA+TGG | 0.570091 | 3.3:+93141171 | None:intergenic |
| CTCCACGTCTCAGTAAAGAA+AGG | 0.574685 | 3.3:+93141922 | None:intergenic |
| CATCTGTGATGAATGCAATT+GGG | 0.578682 | 3.3:-93141990 | MS.gene69410:CDS |
| GAGCAAGATATCACACTCAA+AGG | 0.583825 | 3.3:-93142399 | MS.gene69410:CDS |
| GGTTACAGATATTCGGGTGA+AGG | 0.584730 | 3.3:-93141472 | MS.gene69410:intron |
| GTTGTCCGAGCTAAGAAGAT+GGG | 0.586718 | 3.3:-93142039 | MS.gene69410:CDS |
| ACACTCGGTATCTTCTGTGT+AGG | 0.591347 | 3.3:+93140234 | None:intergenic |
| CTAGAGGATGATGTCTCAGA+TGG | 0.593838 | 3.3:+93133221 | None:intergenic |
| AAGCTTTAGTATGAGAATGA+GGG | 0.597235 | 3.3:-93138927 | MS.gene69410:intron |
| ATGAATGCAATTGGGATCTG+TGG | 0.605367 | 3.3:-93141982 | MS.gene69410:CDS |
| TCATCCTCTAGATTAGGAAG+TGG | 0.605694 | 3.3:-93133209 | MS.gene69410:CDS |
| ATTTCGGCAAATGCATGGAA+TGG | 0.607023 | 3.3:+93133088 | None:intergenic |
| AAAATGAACTACATTGGCAA+AGG | 0.607181 | 3.3:+93138878 | None:intergenic |
| ACGAGCAAGCCCAACACACT+CGG | 0.610208 | 3.3:+93140219 | None:intergenic |
| CTGCACAAAGATTCAAGTGT+CGG | 0.611741 | 3.3:+93141447 | None:intergenic |
| CAAAAGGATCACCAACAACA+AGG | 0.612297 | 3.3:+93142181 | None:intergenic |
| TCCTTCGGCCTCTCTTCACA+CGG | 0.614104 | 3.3:-93142318 | MS.gene69410:CDS |
| AAAGCTGTCATCATCACTGT+CGG | 0.614662 | 3.3:-93133285 | MS.gene69410:intron |
| GAACTACATTGGCAAAGGTG+AGG | 0.621693 | 3.3:+93138883 | None:intergenic |
| TCTACAGGTAGTGATGAGAA+TGG | 0.622747 | 3.3:+93133254 | None:intergenic |
| TCTTCTTAGCTCGGACAACA+AGG | 0.625706 | 3.3:+93142043 | None:intergenic |
| GTTATTACTAGGCAATGCAA+AGG | 0.632024 | 3.3:+93140127 | None:intergenic |
| TGTACCACTTCCTAATCTAG+AGG | 0.640795 | 3.3:+93133205 | None:intergenic |
| GCCGTGTGAAGAGAGGCCGA+AGG | 0.658038 | 3.3:+93142317 | None:intergenic |
| TATGGAAGAAGAAAATCACT+GGG | 0.681392 | 3.3:+93133044 | None:intergenic |
| TTTATAGTCATATATCTGGG+AGG | 0.687291 | 3.3:+93141199 | None:intergenic |
| AAGGTGAGGATGTGGCTACG+AGG | 0.741336 | 3.3:+93138897 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 93133019 | 93142454 | 93133019 | ID=MS.gene69410 |
| chr3.3 | mRNA | 93133019 | 93142454 | 93133019 | ID=MS.gene69410.t1;Parent=MS.gene69410 |
| chr3.3 | exon | 93142180 | 93142454 | 93142180 | ID=MS.gene69410.t1.exon1;Parent=MS.gene69410.t1 |
| chr3.3 | CDS | 93142180 | 93142454 | 93142180 | ID=cds.MS.gene69410.t1;Parent=MS.gene69410.t1 |
| chr3.3 | exon | 93141854 | 93142083 | 93141854 | ID=MS.gene69410.t1.exon2;Parent=MS.gene69410.t1 |
| chr3.3 | CDS | 93141854 | 93142083 | 93141854 | ID=cds.MS.gene69410.t1;Parent=MS.gene69410.t1 |
| chr3.3 | exon | 93141447 | 93141486 | 93141447 | ID=MS.gene69410.t1.exon3;Parent=MS.gene69410.t1 |
| chr3.3 | CDS | 93141447 | 93141486 | 93141447 | ID=cds.MS.gene69410.t1;Parent=MS.gene69410.t1 |
| chr3.3 | exon | 93141149 | 93141240 | 93141149 | ID=MS.gene69410.t1.exon4;Parent=MS.gene69410.t1 |
| chr3.3 | CDS | 93141149 | 93141240 | 93141149 | ID=cds.MS.gene69410.t1;Parent=MS.gene69410.t1 |
| chr3.3 | exon | 93140135 | 93140256 | 93140135 | ID=MS.gene69410.t1.exon5;Parent=MS.gene69410.t1 |
| chr3.3 | CDS | 93140135 | 93140256 | 93140135 | ID=cds.MS.gene69410.t1;Parent=MS.gene69410.t1 |
| chr3.3 | exon | 93138839 | 93138940 | 93138839 | ID=MS.gene69410.t1.exon6;Parent=MS.gene69410.t1 |
| chr3.3 | CDS | 93138839 | 93138940 | 93138839 | ID=cds.MS.gene69410.t1;Parent=MS.gene69410.t1 |
| chr3.3 | exon | 93133019 | 93133303 | 93133019 | ID=MS.gene69410.t1.exon7;Parent=MS.gene69410.t1 |
| chr3.3 | CDS | 93133019 | 93133303 | 93133019 | ID=cds.MS.gene69410.t1;Parent=MS.gene69410.t1 |
| Gene Sequence |
| Protein sequence |
