AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86968

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86968.t1 MTR_5g072530 100.000 88 0 0 1 88 1 88 4.62e-60 177
MS.gene86968.t1 MTR_2g104440 100.000 88 0 0 1 88 1 88 4.62e-60 177
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86968.t1 AT4G30330 89.773 88 9 0 1 88 1 88 4.45e-55 165
MS.gene86968.t1 AT2G18740 89.773 88 9 0 1 88 1 88 5.93e-55 165
MS.gene86968.t1 AT2G18740 87.324 71 9 0 1 71 1 71 2.53e-42 133

Find 13 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATACATGAATTTGGTTT+TGG 0.297744 2.3:-432732 MS.gene86968:CDS
TCAGGGAGGATCCTTCTTAA+AGG 0.324647 2.3:-432585 MS.gene86968:intron
GCAAAGCGCGGATTCAGATT+TGG 0.362553 2.3:-435944 MS.gene86968:CDS
CAAAAGGATTTGAGGATTGA+AGG 0.404712 2.3:-435912 MS.gene86968:CDS
GGTCATGACCCTTTGAACTT+TGG 0.450628 2.3:+436079 None:intergenic
TTATGTTGTCTCCTTTAAGA+AGG 0.459912 2.3:+432574 None:intergenic
ACAAATTTCAGGGGTAAATG+AGG 0.483171 2.3:-432457 None:intergenic
GAGAAAGCATACAATGGGTT+GGG 0.488064 2.3:+436058 None:intergenic
GGCGAGCACCAAAGTTCAAA+GGG 0.524848 2.3:-436087 MS.gene86968:CDS
TTGAACTTTGGTGCTCGCCA+TGG 0.529943 2.3:+436091 None:intergenic
TGAGAAAGCATACAATGGGT+TGG 0.551589 2.3:+436057 None:intergenic
TGGCGAGCACCAAAGTTCAA+AGG 0.558128 2.3:-436088 MS.gene86968:CDS
GATTTCTTCAAAGCAAAGCG+CGG 0.674013 2.3:-435956 MS.gene86968:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAAATCATTGTTTTTTA+TGG - chr2.3:432696-432715 MS.gene86968:CDS 10.0%
!!! TTTTCTTTTTGTTATAAATA+GGG - chr2.3:435787-435806 MS.gene86968:intron 10.0%
!!! TTTTTAGATTTTTAATGAAT+TGG - chr2.3:433682-433701 MS.gene86968:intron 10.0%
!!! TTTTTCTTTTTGTTATAAAT+AGG - chr2.3:435786-435805 MS.gene86968:intron 10.0%
!! AAGAATTAAATATTAGAAGT+TGG + chr2.3:433712-433731 None:intergenic 15.0%
!! AGAATTAAATATTAGAAGTT+GGG + chr2.3:433711-433730 None:intergenic 15.0%
!! CTATATATAAAAAGGATATA+TGG + chr2.3:434171-434190 None:intergenic 15.0%
!!! CTTGTTATTCATATAAAAAA+GGG - chr2.3:433370-433389 MS.gene86968:intron 15.0%
!!! TCTTGTTATTCATATAAAAA+AGG - chr2.3:433369-433388 MS.gene86968:intron 15.0%
!! AAATTAATAAACCAAAAGAC+AGG + chr2.3:435103-435122 None:intergenic 20.0%
!! AACTTTAATACTAACTTGAA+AGG - chr2.3:435420-435439 MS.gene86968:intron 20.0%
!! AATTTGTAAATTAGTTATGC+TGG + chr2.3:434873-434892 None:intergenic 20.0%
!! ATTTGTAAATTAGTTATGCT+GGG + chr2.3:434872-434891 None:intergenic 20.0%
!! GAATTAAATATTAGAAGTTG+GGG + chr2.3:433710-433729 None:intergenic 20.0%
!! TTGTTAAAGAGAAATTGAAA+GGG + chr2.3:432528-432547 None:intergenic 20.0%
!!! AAATGTTTGCTTATTGTTTT+TGG - chr2.3:435700-435719 MS.gene86968:intron 20.0%
!!! AAGTTCTGAGAAAAAAAAAA+CGG + chr2.3:432564-432583 None:intergenic 20.0%
!!! ATAAAAAAGGGTATATTGAT+GGG - chr2.3:433382-433401 MS.gene86968:intron 20.0%
!!! ATTTGCTTTTACAAATTTCA+GGG - chr2.3:436081-436100 MS.gene86968:CDS 20.0%
!!! CCTAAAAAAATTTGTTTCTT+GGG + chr2.3:433584-433603 None:intergenic 20.0%
!!! GCTAGTTTCTAATAAAAAAA+GGG + chr2.3:433757-433776 None:intergenic 20.0%
!!! TATAAAAAAGGGTATATTGA+TGG - chr2.3:433381-433400 MS.gene86968:intron 20.0%
!!! TGAATCTAGCTAATTTTATA+GGG + chr2.3:434589-434608 None:intergenic 20.0%
!!! TGCTAGTTTCTAATAAAAAA+AGG + chr2.3:433758-433777 None:intergenic 20.0%
!!! TTAGATCTTATTTTCAGTTA+AGG + chr2.3:434249-434268 None:intergenic 20.0%
!!! TTCAAGTTAGTATTAAAGTT+AGG + chr2.3:435420-435439 None:intergenic 20.0%
!!! TTTTGATGAATACATGAATT+TGG - chr2.3:435810-435829 MS.gene86968:intron 20.0%
! AAAAGAGGAGAAAAATGAAA+AGG + chr2.3:433280-433299 None:intergenic 25.0%
! AATGAAAAGGAAAGAGATAA+AGG + chr2.3:433267-433286 None:intergenic 25.0%
! AATGATATATGATTGTCTTG+AGG - chr2.3:433003-433022 MS.gene86968:intron 25.0%
! AGAAAAAAAAAACGGAGATT+AGG + chr2.3:432556-432575 None:intergenic 25.0%
! AGACAATCATATATCATTTC+AGG + chr2.3:433001-433020 None:intergenic 25.0%
! AGTTACTAGAAAATGAATGA+TGG + chr2.3:435017-435036 None:intergenic 25.0%
! GATGTTCTTGTATGAAAAAT+GGG - chr2.3:434441-434460 MS.gene86968:intron 25.0%
! GTTGTTAAAGAGAAATTGAA+AGG + chr2.3:432529-432548 None:intergenic 25.0%
! TGATGTTCTTGTATGAAAAA+TGG - chr2.3:434440-434459 MS.gene86968:intron 25.0%
! TGGTTGTTTAATTATGGATT+TGG + chr2.3:433096-433115 None:intergenic 25.0%
! TTTCAAATTGGATATTTGAG+TGG - chr2.3:435745-435764 MS.gene86968:intron 25.0%
!! AATCTAGCTAATTTTATAGG+GGG + chr2.3:434587-434606 None:intergenic 25.0%
!! AGGTTTTCAGTTATTTAGAT+CGG + chr2.3:434229-434248 None:intergenic 25.0%
!! ATTTCAAAATGTTGCGATTT+TGG - chr2.3:432944-432963 MS.gene86968:intron 25.0%
!! GAATCTAGCTAATTTTATAG+GGG + chr2.3:434588-434607 None:intergenic 25.0%
!! GAGATTCAAATTCAAAGTTT+TGG + chr2.3:435614-435633 None:intergenic 25.0%
!! GCCTAAAAAAATTTGTTTCT+TGG + chr2.3:433585-433604 None:intergenic 25.0%
!! GTGAATCTAGCTAATTTTAT+AGG + chr2.3:434590-434609 None:intergenic 25.0%
!! TAAAAAAGGGTATATTGATG+GGG - chr2.3:433383-433402 MS.gene86968:intron 25.0%
!! TGAATCTAGCTAATTTTACA+TGG + chr2.3:432849-432868 None:intergenic 25.0%
!!! ATCGCAACATTTTGAAATTT+AGG + chr2.3:432942-432961 None:intergenic 25.0%
!!! ATCGGTTTGTCATTTTAATT+TGG + chr2.3:434211-434230 None:intergenic 25.0%
!!! ATGATGTTATTGTTTTGATC+AGG - chr2.3:435945-435964 MS.gene86968:CDS 25.0%
!!! ATTCAAAGTTTTGGATAACA+TGG + chr2.3:435605-435624 None:intergenic 25.0%
!!! CCCAAGAAACAAATTTTTTT+AGG - chr2.3:433581-433600 MS.gene86968:intron 25.0%
!!! GATTTGCTTTTACAAATTTC+AGG - chr2.3:436080-436099 MS.gene86968:CDS 25.0%
!!! GCATTTTCATCTATTTCTAT+GGG - chr2.3:436031-436050 MS.gene86968:intron 25.0%
!!! TCGCAACATTTTGAAATTTA+GGG + chr2.3:432941-432960 None:intergenic 25.0%
!!! TGAATACATGAATTTGGTTT+TGG - chr2.3:435816-435835 MS.gene86968:intron 25.0%
!!! TGATGTTATTGTTTTGATCA+GGG - chr2.3:435946-435965 MS.gene86968:CDS 25.0%
!!! TGCATTTTCATCTATTTCTA+TGG - chr2.3:436030-436049 MS.gene86968:intron 25.0%
!!! TGTTGTATTTGCTATATTGT+TGG + chr2.3:433916-433935 None:intergenic 25.0%
!!! TTGTCTGTTTTTCTTTTTTG+TGG - chr2.3:434012-434031 MS.gene86968:intron 25.0%
!!! TTTATGGCATGTTTTAGAAT+AGG - chr2.3:432712-432731 MS.gene86968:CDS 25.0%
!!! TTTGCTTTTACAAATTTCAG+GGG - chr2.3:436082-436101 MS.gene86968:CDS 25.0%
AAATTGGATATTTGAGTGGT+TGG - chr2.3:435749-435768 MS.gene86968:intron 30.0%
AAGAAACAGTGACAAAAAAG+AGG + chr2.3:433295-433314 None:intergenic 30.0%
AATTTCAGATCATCTCTTCT+AGG + chr2.3:435348-435367 None:intergenic 30.0%
ACATGGTACTCATTTCAAAT+TGG - chr2.3:435733-435752 MS.gene86968:intron 30.0%
ACTAGAAAATGAATGATGGT+TGG + chr2.3:435013-435032 None:intergenic 30.0%
AGTTATTTGGATTCTGATTC+TGG - chr2.3:435171-435190 MS.gene86968:intron 30.0%
ATACGTAGGTATATGATGTT+TGG + chr2.3:434379-434398 None:intergenic 30.0%
ATTCATGAGCTCATTTCTTA+GGG - chr2.3:435675-435694 MS.gene86968:intron 30.0%
CATACTAGTACAAAATACGT+AGG + chr2.3:434393-434412 None:intergenic 30.0%
GAAACAAGAGATTAAAGAAG+AGG + chr2.3:433319-433338 None:intergenic 30.0%
GAATAACCAAACACCATTAA+TGG + chr2.3:433235-433254 None:intergenic 30.0%
GAATTTCGTATTTCAAACTG+AGG - chr2.3:435442-435461 MS.gene86968:intron 30.0%
GAGTGTAATATTTCTTAGAG+AGG + chr2.3:435543-435562 None:intergenic 30.0%
GCAATAAAAATGACGAACAA+GGG + chr2.3:433785-433804 None:intergenic 30.0%
GGAGCATAATGGTTATTTAT+TGG - chr2.3:432896-432915 MS.gene86968:intron 30.0%
TGCAATAAAAATGACGAACA+AGG + chr2.3:433786-433805 None:intergenic 30.0%
TGCAGTATATATGTAAGATC+CGG + chr2.3:434058-434077 None:intergenic 30.0%
TTCCAAATCGATAAACTACA+AGG + chr2.3:435045-435064 None:intergenic 30.0%
TTCTTATCTCTTCTTTCAGA+GGG + chr2.3:435490-435509 None:intergenic 30.0%
TTTCTTATCTCTTCTTTCAG+AGG + chr2.3:435491-435510 None:intergenic 30.0%
TTTGATACCTAGAGTTATTG+AGG + chr2.3:432797-432816 None:intergenic 30.0%
TTTGTCTCTTTCAACAGTTT+AGG - chr2.3:435635-435654 MS.gene86968:intron 30.0%
! AACCTTGTAGTTTATCGATT+TGG - chr2.3:435040-435059 MS.gene86968:intron 30.0%
! ATAGTGTTTTGCACAATACA+AGG + chr2.3:434744-434763 None:intergenic 30.0%
! ATATGGTTTAGTGCCATTAA+TGG - chr2.3:433219-433238 MS.gene86968:intron 30.0%
! TTATGGATTTGGTATTCACT+TGG + chr2.3:433085-433104 None:intergenic 30.0%
! TTATGTTGTCTCCTTTAAGA+AGG + chr2.3:435977-435996 None:intergenic 30.0%
!! ACGTGTGTGAAAGTTTTAAT+AGG + chr2.3:432973-432992 None:intergenic 30.0%
!! CGTGTGTGAAAGTTTTAATA+GGG + chr2.3:432972-432991 None:intergenic 30.0%
!! GTGTGTGAAAGTTTTAATAG+GGG + chr2.3:432971-432990 None:intergenic 30.0%
!! GTTTGTTGATGGTTTACATT+GGG + chr2.3:434813-434832 None:intergenic 30.0%
!! TCTGTTTTTCTTTTTTGTGG+TGG - chr2.3:434015-434034 MS.gene86968:intron 30.0%
!! TGTCTTATTTGTGGATGTTA+GGG - chr2.3:435304-435323 MS.gene86968:intron 30.0%
!! TGTGTGAAAGTTTTAATAGG+GGG + chr2.3:432970-432989 None:intergenic 30.0%
!! TTTTTGAGCAAAAGGATTTG+AGG - chr2.3:432628-432647 MS.gene86968:intron 30.0%
!! TTTTTGGTATAGAGTGTACA+TGG - chr2.3:435716-435735 MS.gene86968:intron 30.0%
!!! ACTTAGGATTGGTTAGTTTT+TGG - chr2.3:435273-435292 MS.gene86968:intron 30.0%
!!! AGTTTTGGATAACATGGATT+TGG + chr2.3:435599-435618 None:intergenic 30.0%
!!! CCATTGTTTTAAACATGTCT+CGG + chr2.3:434664-434683 None:intergenic 30.0%
!!! CGCAACATTTTGAAATTTAG+GGG + chr2.3:432940-432959 None:intergenic 30.0%
!!! CTTAGGATTGGTTAGTTTTT+GGG - chr2.3:435274-435293 MS.gene86968:intron 30.0%
!!! GCAACATTTTGAAATTTAGG+GGG + chr2.3:432939-432958 None:intergenic 30.0%
!!! TTAGGATTGGTTAGTTTTTG+GGG - chr2.3:435275-435294 MS.gene86968:intron 30.0%
AAGAAGAGCAAAAAGACACT+AGG - chr2.3:435865-435884 MS.gene86968:intron 35.0%
AATAAACTACAAGGCTGAGT+TGG + chr2.3:434959-434978 None:intergenic 35.0%
AATCCTAAGTTTATGAGCTG+GGG + chr2.3:435263-435282 None:intergenic 35.0%
ACCGATTAAGCAAGCTAAAA+AGG - chr2.3:433636-433655 MS.gene86968:intron 35.0%
ATAAACTACAAGGCTGAGTT+GGG + chr2.3:434958-434977 None:intergenic 35.0%
ATCAACCACTGTATAGACTA+TGG - chr2.3:435371-435390 MS.gene86968:intron 35.0%
ATGTAAACCATCAACAAACG+AGG - chr2.3:434814-434833 MS.gene86968:intron 35.0%
CAAAAGGATTTGAGGATTGA+AGG - chr2.3:432636-432655 MS.gene86968:intron 35.0%
CAATCCTAAGTTTATGAGCT+GGG + chr2.3:435264-435283 None:intergenic 35.0%
CACCTTTGTGTAAAAGTCTA+TGG - chr2.3:433167-433186 MS.gene86968:intron 35.0%
CAGAAAATGCATATGTTGCA+AGG + chr2.3:435924-435943 None:intergenic 35.0%
CAGTATATATGTAAGATCCG+GGG + chr2.3:434056-434075 None:intergenic 35.0%
CCGATTAAGCAAGCTAAAAA+GGG - chr2.3:433637-433656 MS.gene86968:intron 35.0%
CCTTCAAAATCCAAAACTTG+AGG - chr2.3:433120-433139 MS.gene86968:intron 35.0%
GAATTCTGATGCTATGATTG+TGG - chr2.3:435136-435155 MS.gene86968:intron 35.0%
GATTCATGAGCTCATTTCTT+AGG - chr2.3:435674-435693 MS.gene86968:intron 35.0%
GATTCGTTGCTCTAGTTATT+TGG - chr2.3:435158-435177 MS.gene86968:intron 35.0%
GCAGTATATATGTAAGATCC+GGG + chr2.3:434057-434076 None:intergenic 35.0%
GGTGCTTGGTTGTTTAATTA+TGG + chr2.3:433102-433121 None:intergenic 35.0%
TAAAGACGCAATATTCGATC+TGG - chr2.3:433497-433516 MS.gene86968:intron 35.0%
TCTGCATTGTCATTCTATTG+CGG - chr2.3:433337-433356 MS.gene86968:intron 35.0%
TCTTATCTCTTCTTTCAGAG+GGG + chr2.3:435489-435508 None:intergenic 35.0%
TGCACTGTCTACATTTATCT+TGG - chr2.3:435573-435592 MS.gene86968:intron 35.0%
TGGCACTAAACCATATTTCA+AGG + chr2.3:433215-433234 None:intergenic 35.0%
TGGTGTTTGGTTATTCTCTT+CGG - chr2.3:433239-433258 MS.gene86968:intron 35.0%
TTCATGAGCTCATTTCTTAG+GGG - chr2.3:435676-435695 MS.gene86968:intron 35.0%
! AGTGATGAGAAAGCATACAA+TGG + chr2.3:432499-432518 None:intergenic 35.0%
! ATTCACCAAGTTAGTCTGTT+AGG + chr2.3:434932-434951 None:intergenic 35.0%
! CCGAGACATGTTTAAAACAA+TGG - chr2.3:434661-434680 MS.gene86968:intron 35.0%
! GATATTTGAGTGGTTGGTTA+AGG - chr2.3:435755-435774 MS.gene86968:intron 35.0%
! GTCACTATGCTTTTCAATCT+CGG - chr2.3:432748-432767 MS.gene86968:intron 35.0%
! GTCCATAGACTTTTACACAA+AGG + chr2.3:433172-433191 None:intergenic 35.0%
! GTGATGAGAAAGCATACAAT+GGG + chr2.3:432498-432517 None:intergenic 35.0%
! GTTTGTTAAGAAACCCCAAA+TGG - chr2.3:434693-434712 MS.gene86968:intron 35.0%
! TTAGTGCCATTAATGGTGTT+TGG - chr2.3:433226-433245 MS.gene86968:intron 35.0%
!! AGACTTTTACACAAAGGTGT+TGG + chr2.3:433166-433185 None:intergenic 35.0%
!! CGTTTGTTGATGGTTTACAT+TGG + chr2.3:434814-434833 None:intergenic 35.0%
!! CTGTCTTATTTGTGGATGTT+AGG - chr2.3:435303-435322 MS.gene86968:intron 35.0%
!! GTTTTTCTTTTTTGTGGTGG+TGG - chr2.3:434018-434037 MS.gene86968:intron 35.0%
!!! ACTTTTACACAAAGGTGTTG+GGG + chr2.3:433164-433183 None:intergenic 35.0%
!!! ATGGCATGTTTTAGAATAGG+AGG - chr2.3:432715-432734 MS.gene86968:CDS 35.0%
!!! CAATAAGCTGTTTTTGCATG+CGG - chr2.3:433547-433566 MS.gene86968:intron 35.0%
!!! CCCTTTTTAGCTTGCTTAAT+CGG + chr2.3:433640-433659 None:intergenic 35.0%
!!! CCTCAAGTTTTGGATTTTGA+AGG + chr2.3:433123-433142 None:intergenic 35.0%
!!! GACTTTTACACAAAGGTGTT+GGG + chr2.3:433165-433184 None:intergenic 35.0%
!!! GATTTGGCTTTTTGAGCAAA+AGG - chr2.3:432620-432639 MS.gene86968:intron 35.0%
!!! TGTTATTGTTTTGATCAGGG+AGG - chr2.3:435949-435968 MS.gene86968:CDS 35.0%
!!! TTTTGGATTTTGAAGGTGCT+TGG + chr2.3:433116-433135 None:intergenic 35.0%
AAATGAATGATGGTTGGACG+AGG + chr2.3:435007-435026 None:intergenic 40.0%
AAATGCATGGATTTGACACG+TGG - chr2.3:433428-433447 MS.gene86968:intron 40.0%
AACCACTGTATAGACTATGG+AGG - chr2.3:435374-435393 MS.gene86968:intron 40.0%
AGGCAATTTGTGTTCCTCTT+TGG - chr2.3:433053-433072 MS.gene86968:intron 40.0%
ATTAGTTATGCTGGGAGCTA+TGG + chr2.3:434864-434883 None:intergenic 40.0%
ATTGATGGGGTGGTGAAAAT+GGG - chr2.3:433396-433415 MS.gene86968:intron 40.0%
CCAATCCTAAGTTTATGAGC+TGG + chr2.3:435265-435284 None:intergenic 40.0%
CCAGCTCATAAACTTAGGAT+TGG - chr2.3:435262-435281 MS.gene86968:intron 40.0%
GACTTGGCGAATAAACTACA+AGG + chr2.3:434968-434987 None:intergenic 40.0%
GAGTGCTACAACTGTAAGAT+AGG + chr2.3:434767-434786 None:intergenic 40.0%
GATTTCTTCAAAGCAAAGCG+CGG - chr2.3:432592-432611 MS.gene86968:intron 40.0%
TAAACCATCAACAAACGAGG+TGG - chr2.3:434817-434836 MS.gene86968:intron 40.0%
TATTGATGGGGTGGTGAAAA+TGG - chr2.3:433395-433414 MS.gene86968:intron 40.0%
TCATGTGCCTCAATAACTCT+AGG - chr2.3:432787-432806 MS.gene86968:intron 40.0%
TGAATAAGCCCATTATTGCG+AGG - chr2.3:434901-434920 MS.gene86968:intron 40.0%
TGGGAAAGAGGAAAAATGCA+TGG - chr2.3:433415-433434 MS.gene86968:intron 40.0%
TTAGTTATGCTGGGAGCTAT+GGG + chr2.3:434863-434882 None:intergenic 40.0%
TTGCACCTAACAGACTAACT+TGG - chr2.3:434924-434943 MS.gene86968:intron 40.0%
! AAAAGGGTATATTGATGGGG+TGG - chr2.3:433386-433405 MS.gene86968:intron 40.0%
! ATTCTGATTCTGGTTAGACC+AGG - chr2.3:435181-435200 MS.gene86968:intron 40.0%
! CAATACAAGGCATTACCCTT+CGG + chr2.3:434731-434750 None:intergenic 40.0%
! CTCTGCATAGCCTTGAAATA+TGG - chr2.3:433202-433221 MS.gene86968:intron 40.0%
! GAGAAAGCATACAATGGGTT+GGG + chr2.3:432493-432512 None:intergenic 40.0%
! TGAACATGTCTGGAGCATAA+TGG - chr2.3:432885-432904 MS.gene86968:intron 40.0%
! TGAGAAAGCATACAATGGGT+TGG + chr2.3:432494-432513 None:intergenic 40.0%
!!! TGGGGTTTGTTTTGATTGAG+AGG + chr2.3:433146-433165 None:intergenic 40.0%
AAGGCTATGCAGAGATGTGA+AGG + chr2.3:433196-433215 None:intergenic 45.0%
ACCTGATGCTCAACATGACT+GGG + chr2.3:433031-433050 None:intergenic 45.0%
AGGTGCAACCTCGCAATAAT+GGG + chr2.3:434912-434931 None:intergenic 45.0%
CAAAAGACAGGTTCACAACC+TGG + chr2.3:435091-435110 None:intergenic 45.0%
CTAAGTTTATGAGCTGGGGT+GGG + chr2.3:435259-435278 None:intergenic 45.0%
GATGGTTTACATTGGGCTGT+TGG + chr2.3:434806-434825 None:intergenic 45.0%
GCTCATATTAACCGCATCTG+TGG - chr2.3:433603-433622 MS.gene86968:intron 45.0%
GGTCATGACCCTTTGAACTT+TGG + chr2.3:432472-432491 None:intergenic 45.0%
GTATACGTGTGCGAATGTGT+TGG - chr2.3:433473-433492 MS.gene86968:intron 45.0%
TAGCTAGATTCACGAGCTTG+AGG - chr2.3:432857-432876 MS.gene86968:intron 45.0%
TAGGTGCAACCTCGCAATAA+TGG + chr2.3:434913-434932 None:intergenic 45.0%
TAGTTATGCTGGGAGCTATG+GGG + chr2.3:434862-434881 None:intergenic 45.0%
TCAGGGAGGATCCTTCTTAA+AGG - chr2.3:435963-435982 MS.gene86968:CDS 45.0%
TCATGTTGAGCATCAGGTGA+AGG - chr2.3:433033-433052 MS.gene86968:intron 45.0%
TGAGCTTAGCTCAGCTGATA+TGG + chr2.3:434085-434104 None:intergenic 45.0%
TGAGGACGATTGAACATGTC+TGG - chr2.3:432875-432894 MS.gene86968:intron 45.0%
TGCCTCCATAGTCTATACAG+TGG + chr2.3:435379-435398 None:intergenic 45.0%
TTATGCTGTTGTTGTGTGCC+AGG - chr2.3:435070-435089 MS.gene86968:intron 45.0%
! ACATCCACCTCGTTTGTTGA+TGG + chr2.3:434824-434843 None:intergenic 45.0%
! CAGGTTGTGAACCTGTCTTT+TGG - chr2.3:435089-435108 MS.gene86968:intron 45.0%
! GATTGAGAGGCCTCAAGTTT+TGG + chr2.3:433133-433152 None:intergenic 45.0%
!! GGGTTCAGCTGTCTTATTTG+TGG - chr2.3:435295-435314 MS.gene86968:intron 45.0%
!!! TTCTTTTTTGTGGTGGTGGC+CGG - chr2.3:434022-434041 MS.gene86968:intron 45.0%
!! AATATATACTATATATAAAA+AGG + chr2.3:434179-434198 None:intergenic 5.0%
!!! AAAATTTTGATTAAAATTAT+AGG - chr2.3:433832-433851 MS.gene86968:intron 5.0%
AACGAGGTGGATGTGTTGTC+TGG - chr2.3:434830-434849 MS.gene86968:intron 50.0%
ATTCGCCAAGTCAGCCTGTT+AGG - chr2.3:434976-434995 MS.gene86968:intron 50.0%
CACCTGATGCTCAACATGAC+TGG + chr2.3:433032-433051 None:intergenic 50.0%
CCACCCCAGCTCATAAACTT+AGG - chr2.3:435257-435276 MS.gene86968:intron 50.0%
CCTAAGTTTATGAGCTGGGG+TGG + chr2.3:435260-435279 None:intergenic 50.0%
CGCAATATTCGATCTGGACG+AGG - chr2.3:433503-433522 MS.gene86968:intron 50.0%
GAGCTAGATTCACGAGCTTG+AGG - chr2.3:434474-434493 MS.gene86968:intron 50.0%
GCAAAGCGCGGATTCAGATT+TGG - chr2.3:432604-432623 MS.gene86968:intron 50.0%
GGCGAGCACCAAAGTTCAAA+GGG - chr2.3:432461-432480 MS.gene86968:intron 50.0%
GGGTGGTGAAAATGGGAAAG+AGG - chr2.3:433403-433422 MS.gene86968:intron 50.0%
TAGTCTGGTCTGGTTAGACC+AGG - chr2.3:435220-435239 MS.gene86968:intron 50.0%
TCAGCTGAGCTAAGCTCACA+AGG - chr2.3:434087-434106 MS.gene86968:intron 50.0%
TCCCAGTCATGTTGAGCATC+AGG - chr2.3:433027-433046 MS.gene86968:intron 50.0%
TGGCGAGCACCAAAGTTCAA+AGG - chr2.3:432460-432479 MS.gene86968:intron 50.0%
TGGGCTGTTGGATGCATACA+TGG + chr2.3:434794-434813 None:intergenic 50.0%
TTGCACCTAACAGGCTGACT+TGG + chr2.3:434984-435003 None:intergenic 50.0%
! GCTCGTGAATCTAGCTCAAG+AGG + chr2.3:434471-434490 None:intergenic 50.0%
! GCTGTTGCATCTGCCATTTG+GGG + chr2.3:434709-434728 None:intergenic 50.0%
! GGCTGTTGCATCTGCCATTT+GGG + chr2.3:434710-434729 None:intergenic 50.0%
!! TCACTTGGTGCAGTCCAAAG+AGG + chr2.3:433070-433089 None:intergenic 50.0%
!!! TCTTTTTTGTGGTGGTGGCC+GGG - chr2.3:434023-434042 MS.gene86968:intron 50.0%
AACCGCATCTGTGGCAGTGA+TGG - chr2.3:433612-433631 MS.gene86968:intron 55.0%
AGACCAGACTAGAGCAGACC+TGG + chr2.3:435211-435230 None:intergenic 55.0%
AGGTCTGCTCTAGTCTGGTC+TGG - chr2.3:435210-435229 MS.gene86968:intron 55.0%
ATGGCAGATGCAACAGCCGA+AGG - chr2.3:434712-434731 MS.gene86968:intron 55.0%
CGGCTGTTGCATCTGCCATT+TGG + chr2.3:434711-434730 None:intergenic 55.0%
TCGATCTGGACGAGGTTGCT+TGG - chr2.3:433511-433530 MS.gene86968:intron 55.0%
TGCCATCACTGCCACAGATG+CGG + chr2.3:433617-433636 None:intergenic 55.0%
TGGACGAGGTTGCACCTAAC+AGG + chr2.3:434993-435012 None:intergenic 55.0%
TGGCAGATGCAACAGCCGAA+GGG - chr2.3:434713-434732 MS.gene86968:intron 55.0%
!!! CTTTTTTGTGGTGGTGGCCG+GGG - chr2.3:434024-434043 MS.gene86968:intron 55.0%
AAGATCCGGGGTTCGAACCC+CGG + chr2.3:434044-434063 None:intergenic 60.0%
CAGACTAGAGCAGACCTGGC+TGG + chr2.3:435207-435226 None:intergenic 60.0%
CAGCCAGGTCTGCTCTAGTC+TGG - chr2.3:435205-435224 MS.gene86968:intron 60.0%
CTGGTTAGACCAGGCCAGCC+AGG - chr2.3:435190-435209 MS.gene86968:intron 65.0%
! TAGAGCAGACCTGGCTGGCC+TGG + chr2.3:435202-435221 None:intergenic 65.0%
TGAGCTGGGGTGGGCAGACG+TGG + chr2.3:435250-435269 None:intergenic 70.0%
CTGGGGTGGGCAGACGTGGC+TGG + chr2.3:435246-435265 None:intergenic 75.0%
GGTGGCCGGGGTTCGAACCC+CGG - chr2.3:434036-434055 MS.gene86968:intron 75.0%
! GTGGGCAGACGTGGCTGGCC+TGG + chr2.3:435241-435260 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 432459 436111 432459 ID=MS.gene86968
chr2.3 mRNA 432459 436111 432459 ID=MS.gene86968.t1;Parent=MS.gene86968
chr2.3 exon 436070 436111 436070 ID=MS.gene86968.t1.exon1;Parent=MS.gene86968.t1
chr2.3 CDS 436070 436111 436070 ID=cds.MS.gene86968.t1;Parent=MS.gene86968.t1
chr2.3 exon 435902 435991 435902 ID=MS.gene86968.t1.exon2;Parent=MS.gene86968.t1
chr2.3 CDS 435902 435991 435902 ID=cds.MS.gene86968.t1;Parent=MS.gene86968.t1
chr2.3 exon 432684 432762 432684 ID=MS.gene86968.t1.exon3;Parent=MS.gene86968.t1
chr2.3 CDS 432684 432762 432684 ID=cds.MS.gene86968.t1;Parent=MS.gene86968.t1
chr2.3 exon 432558 432603 432558 ID=MS.gene86968.t1.exon4;Parent=MS.gene86968.t1
chr2.3 CDS 432558 432603 432558 ID=cds.MS.gene86968.t1;Parent=MS.gene86968.t1
chr2.3 exon 432459 432468 432459 ID=MS.gene86968.t1.exon5;Parent=MS.gene86968.t1
chr2.3 CDS 432459 432468 432459 ID=cds.MS.gene86968.t1;Parent=MS.gene86968.t1
Gene Sequence

>MS.gene86968

ATGGCGAGCACCAAAGTTCAAAGGGTCATGACCCAACCCATTGTATGCTTTCTCATCACTCTCTTTCCCTTTCAATTTCTCTTTAACAACTTAACCTAATCTCCGTTTTTTTTTTCTCAGAACTTGATCTTCAGATTTCTTCAAAGCAAAGCGCGGATTCAGATTTGGCTTTTTGAGCAAAAGGATTTGAGGATTGAAGGAAGAATCATTGTAAGTTTTTTTTTTTTTTTTTTTAGTTTTAAAATCATTGTTTTTTATGGCATGTTTTAGAATAGGAGGAAGAGCACTTGTCACTATGCTTTTCAATCTCGGTGTAGCTAACTCAAAATCATGTGCCTCAATAACTCTAGGTATCAAAATTGAGTTAACTCAATTATAAATCTGTTTCCATGTAAAATTAGCTAGATTCACGAGCTTGAGGACGATTGAACATGTCTGGAGCATAATGGTTATTTATTGGCTAAACTGCACTTTTTGCCCCCTAAATTTCAAAATGTTGCGATTTTGGCCCCCTATTAAAACTTTCACACACGTATTTACCTGAAATGATATATGATTGTCTTGAGGATCCCAGTCATGTTGAGCATCAGGTGAAGGCAATTTGTGTTCCTCTTTGGACTGCACCAAGTGAATACCAAATCCATAATTAAACAACCAAGCACCTTCAAAATCCAAAACTTGAGGCCTCTCAATCAAAACAAACCCCAACACCTTTGTGTAAAAGTCTATGGACTCCTTCACATCTCTGCATAGCCTTGAAATATGGTTTAGTGCCATTAATGGTGTTTGGTTATTCTCTTCGGTTCCTTTATCTCTTTCCTTTTCATTTTTCTCCTCTTTTTTGTCACTGTTTCTTTCCTCTTCTTTAATCTCTTGTTTCTGCATTGTCATTCTATTGCGGAGTATAATTTCTTGTTATTCATATAAAAAAGGGTATATTGATGGGGTGGTGAAAATGGGAAAGAGGAAAAATGCATGGATTTGACACGTGGAAGAAAGATGTAATAGAAGCATGTATACGTGTGCGAATGTGTTGGCTAAAGACGCAATATTCGATCTGGACGAGGTTGCTTGGATCGTACACAACACAATAAGCTGTTTTTGCATGCGGTGCAGCTCTTTCCCAAGAAACAAATTTTTTTAGGCTCATATTAACCGCATCTGTGGCAGTGATGGCACCGATTAAGCAAGCTAAAAAGGGTGTTTTAAGTTGAAAATATTATTTTTTAGATTTTTAATGAATTGGCTCCCCAACTTCTAATATTTAATTCTTTAATCAATGTCGTTAGTATTTACCCTTTTTTTATTAGAAACTAGCATGTGCCCTTGTTCGTCATTTTTATTGCATTAATGTTACGAACAATAGTTTATATAAAATTTTGATTAAAATTATAGGTATAGATATTTTGCATTTTCATATTGTATGACAAACTATACTTTTCTTTTTAGTGATACCAACAATATAGCAAATACAACATAAATTCTTACTTTTTTATTAAATAACAACAACAATCTAGTTTAAATAACAACAACAATCTAGTATTATAAGCAATTTGTCTGTTTTTCTTTTTTGTGGTGGTGGCCGGGGTTCGAACCCCGGATCTTACATATATACTGCATTGTCCATATCAGCTGAGCTAAGCTCACAAGGATAGCAATTTGTTTGTTTAAAAGCATATATAATTGAAAGTGAAAAAGTCAGACAAAAACCATATATCCTTTTTATATATAGTATATATTCAAATTAATCCAAATTAAAATGACAAACCGATCTAAATAACTGAAAACCTTAACTGAAAATAAGATCTAAAAACTTCATAAAATCAAATATTATTAGATTTTGTTGAAGTGTTTTTTGCGAAAATTGTTCAGATTAAATTTTATTTTTTTATTTTAAAAAAATCTAATCAATTCGAACCAAACATCATATACCTACGTATTTTGTACTAGTATGATAGATTGTATAAATTAATCTATTATTTGATGTTCTTGTATGAAAAATGGGTGTACCTCTTGAGCTAGATTCACGAGCTTGAGGACGATTGAACATGTCTGGAGCATAATGGTTATTTATTGGCTAAACTGCACTTTTTGCCCCCTAAATTTCAAAATGTTGCGATTTTGGCCCCCTATAAAATTAGCTAGATTCACGAGCTTGAGGACGATTGAACATGTCTGGAGCATAATGGTTATTTATTTTCTCCGAGACATGTTTAAAACAATGGTGATTTAGTGTTTGTTAAGAAACCCCAAATGGCAGATGCAACAGCCGAAGGGTAATGCCTTGTATTGTGCAAAACACTATCCTATCTTACAGTTGTAGCACTCAATTCCATGTATGCATCCAACAGCCCAATGTAAACCATCAACAAACGAGGTGGATGTGTTGTCTGGAGACAACCCCATAGCTCCCAGCATAACTAATTTACAAATTAGAAAACATGAATAAGCCCATTATTGCGAGGTTGCACCTAACAGACTAACTTGGTGAATGAGCCCAACTCAGCCTTGTAGTTTATTCGCCAAGTCAGCCTGTTAGGTGCAACCTCGTCCAACCATCATTCATTTTCTAGTAACTTTGAACCTTGTAGTTTATCGATTTGGAACTTCTTTATGCTGTTGTTGTGTGCCAGGTTGTGAACCTGTCTTTTGGTTTATTAATTTGTCAGTGAAATTTGAATTCTGATGCTATGATTGTGGATTCGTTGCTCTAGTTATTTGGATTCTGATTCTGGTTAGACCAGGCCAGCCAGGTCTGCTCTAGTCTGGTCTGGTTAGACCAGGCCAGCCACGTCTGCCCACCCCAGCTCATAAACTTAGGATTGGTTAGTTTTTGGGGTTCAGCTGTCTTATTTGTGGATGTTAGGGATTAATTTATAGTTAATTCCTAGAAGAGATGATCTGAAATTAGCATCAACCACTGTATAGACTATGGAGGCAGAACTGTAGTTATTTGTGCCTAACTTTAATACTAACTTGAAAGGAATTTCGTATTTCAAACTGAGGTAGAAAATTATAATACACAATCCCCTCTGAAAGAAGAGATAAGAAATGAATCTGTTGTAGTTATTTGTCTATCAACCTCTCTAAGAAATATTACACTCGTCACTACTTTGCACTGTCTACATTTATCTTGGCCAAATCCATGTTATCCAAAACTTTGAATTTGAATCTCATTTGTCTCTTTCAACAGTTTAGGACTTTGTAAACTTTTTGATTCATGAGCTCATTTCTTAGGGGAAAATGTTTGCTTATTGTTTTTGGTATAGAGTGTACATGGTACTCATTTCAAATTGGATATTTGAGTGGTTGGTTAAGGATCTTGTCTTTTTCTTTTTGTTATAAATAGGGTTTTGATGAATACATGAATTTGGTTTTGGATGATGCTGAAGAAGTAAACGTCAAGAAGAAGAGCAAAAAGACACTAGGTAAGATAAATTTCTCAAAGTTTATCATTTTTGTCCTTGCAACATATGCATTTTCTGAATGATGTTATTGTTTTGATCAGGGAGGATCCTTCTTAAAGGAGACAACATAACTTTGATGATGAACACGTAAGTGAAGTTGAAATTGCATTTTCATCTATTTCTATGGGTTTCTTGTCGTAAATTTAAGTGAAATGATTTGCTTTTACAAATTTCAGGGGTAAATGA
Protein sequence

>MS.gene86968.t1

MASTKVQRVMTQPINLIFRFLQSKARIQIWLFEQKDLRIEGRIIGFDEYMNLVLDDAEEVNVKKKSKKTLGRILLKGDNITLMMNTGK