Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69456.t1 | XP_024633877.1 | 97.3 | 329 | 9 | 0 | 1 | 329 | 1 | 329 | 1.40E-177 | 632.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69456.t1 | P74038 | 45.8 | 260 | 141 | 0 | 70 | 329 | 4 | 263 | 2.3e-59 | 231.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69456.t1 | A0A072VDF7 | 97.3 | 329 | 9 | 0 | 1 | 329 | 1 | 329 | 1.0e-177 | 632.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050496 | MS.gene69456 | 0.806555 | 7.77E-50 | -1.69E-46 |
| MS.gene050504 | MS.gene69456 | 0.80098 | 1.12E-48 | -1.69E-46 |
| MS.gene050876 | MS.gene69456 | 0.84887 | 4.65E-60 | -1.69E-46 |
| MS.gene05087 | MS.gene69456 | 0.80202 | 6.84E-49 | -1.69E-46 |
| MS.gene050904 | MS.gene69456 | 0.850439 | 1.70E-60 | -1.69E-46 |
| MS.gene052486 | MS.gene69456 | 0.807542 | 4.81E-50 | -1.69E-46 |
| MS.gene054954 | MS.gene69456 | 0.804223 | 2.39E-49 | -1.69E-46 |
| MS.gene055751 | MS.gene69456 | 0.808146 | 3.57E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene69456 | 0.816808 | 4.57E-52 | -1.69E-46 |
| MS.gene057687 | MS.gene69456 | 0.8044 | 2.20E-49 | -1.69E-46 |
| MS.gene058335 | MS.gene69456 | 0.803972 | 2.70E-49 | -1.69E-46 |
| MS.gene05912 | MS.gene69456 | 0.831816 | 1.35E-55 | -1.69E-46 |
| MS.gene059865 | MS.gene69456 | 0.803842 | 2.87E-49 | -1.69E-46 |
| MS.gene060703 | MS.gene69456 | 0.831209 | 1.90E-55 | -1.69E-46 |
| MS.gene060762 | MS.gene69456 | 0.805743 | 1.15E-49 | -1.69E-46 |
| MS.gene060778 | MS.gene69456 | 0.804302 | 2.31E-49 | -1.69E-46 |
| MS.gene060982 | MS.gene69456 | 0.803813 | 2.91E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69456.t1 | MTR_3g112310 | 97.264 | 329 | 9 | 0 | 1 | 329 | 1 | 329 | 0.0 | 653 |
| MS.gene69456.t1 | MTR_3g112310 | 97.256 | 328 | 9 | 0 | 1 | 328 | 1 | 328 | 0.0 | 650 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69456.t1 | AT1G45110 | 67.974 | 306 | 89 | 1 | 24 | 329 | 20 | 316 | 3.58e-149 | 427 |
Find 103 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTACAAGTTTATTATTATC+TGG | 0.272068 | 3.3:-92597450 | None:intergenic |
| CTAAGAGAATTAATTGAAAA+TGG | 0.305796 | 3.3:+92599935 | MS.gene69456:CDS |
| TGCATGCCCTTCAATCAATA+TGG | 0.318102 | 3.3:-92599867 | None:intergenic |
| ACGAATAGAAAATTCTTTGC+TGG | 0.338729 | 3.3:-92599825 | None:intergenic |
| TCCACTGATGAATTTACATT+TGG | 0.349806 | 3.3:+92598288 | MS.gene69456:CDS |
| GGGGAAAAGGTAAGTTGCTT+TGG | 0.355232 | 3.3:-92597384 | None:intergenic |
| GACAGCAGAATCTTCCCATT+TGG | 0.355666 | 3.3:+92605636 | MS.gene69456:CDS |
| ACTCGGGATCAAGAAGAAAA+AGG | 0.373239 | 3.3:+92598422 | MS.gene69456:CDS |
| CACCATTGTTCAGGGCTCTC+CGG | 0.379351 | 3.3:+92597800 | MS.gene69456:intron |
| CTTCTTGATCCCGAGTGCTT+TGG | 0.381141 | 3.3:-92598415 | None:intergenic |
| AATCCCAGGTCCATGTGCTT+TGG | 0.393976 | 3.3:+92598239 | MS.gene69456:CDS |
| TTCTGCCATGATTCGAAGAT+AGG | 0.394954 | 3.3:-92603784 | None:intergenic |
| CTGTCAGAAGATCTTCAATA+AGG | 0.409597 | 3.3:-92605618 | None:intergenic |
| GAGGTGGAGCAGAAGGATAA+AGG | 0.414381 | 3.3:-92597405 | None:intergenic |
| AGGTGGAGCAGAAGGATAAA+GGG | 0.418031 | 3.3:-92597404 | None:intergenic |
| GGACCTGGGATTGGAACAAC+AGG | 0.427981 | 3.3:-92598228 | None:intergenic |
| TCGAATCATGGCAGAATCTC+AGG | 0.436566 | 3.3:+92603791 | MS.gene69456:CDS |
| GGCAGTGGAAAACGCCATCC+AGG | 0.443924 | 3.3:-92597325 | None:intergenic |
| ATCTTGAAGACATCACCTTC+AGG | 0.445097 | 3.3:+92597624 | MS.gene69456:CDS |
| CTTTCTCAGTTTCTTGAAGA+GGG | 0.449860 | 3.3:+92598502 | MS.gene69456:CDS |
| TTCCTTCTGGTCTACACAAA+GGG | 0.450091 | 3.3:+92605683 | MS.gene69456:CDS |
| GGTATCGGCTCTTTCTGCCT+CGG | 0.453758 | 3.3:+92598260 | MS.gene69456:CDS |
| GACGGATGTGATCATCCAAA+TGG | 0.459983 | 3.3:-92605651 | None:intergenic |
| AGGTCCATGTGCTTTGGTAT+CGG | 0.460351 | 3.3:+92598245 | MS.gene69456:CDS |
| GTATCGGCTCTTTCTGCCTC+GGG | 0.462242 | 3.3:+92598261 | MS.gene69456:CDS |
| CGGAAGACACGAGGCACTCT+GGG | 0.466378 | 3.3:+92597851 | MS.gene69456:CDS |
| ACCATTGTTCAGGGCTCTCC+GGG | 0.474185 | 3.3:+92597801 | MS.gene69456:intron |
| TCACCCCTATCTTCGAATCA+TGG | 0.474504 | 3.3:+92603779 | MS.gene69456:CDS |
| GCAGAAGGATAAAGGGGAAA+AGG | 0.477021 | 3.3:-92597397 | None:intergenic |
| AATATGGTAAGTTCTCCCTT+TGG | 0.477519 | 3.3:-92599851 | None:intergenic |
| CAGTGGAAGAGGTGCGGCAG+TGG | 0.480861 | 3.3:-92597340 | None:intergenic |
| AAGAAGGGAAAATGTTGCTT+CGG | 0.484632 | 3.3:+92597295 | None:intergenic |
| AAAGCACATGGACCTGGGAT+TGG | 0.488329 | 3.3:-92598237 | None:intergenic |
| TCGGAAGACACGAGGCACTC+TGG | 0.490083 | 3.3:+92597850 | MS.gene69456:CDS |
| GAACTTACCATATTGATTGA+AGG | 0.490717 | 3.3:+92599860 | MS.gene69456:CDS |
| ATAAGTGACGCTGGAACGCC+TGG | 0.495165 | 3.3:+92598066 | MS.gene69456:CDS |
| AAAGGTAAGTTGCTTTGGAA+TGG | 0.495257 | 3.3:-92597379 | None:intergenic |
| AAAGCGTGGCATTTCCTAGC+TGG | 0.499425 | 3.3:+92605712 | MS.gene69456:CDS |
| GTTCCTGTTGTTCCAATCCC+AGG | 0.500390 | 3.3:+92598225 | MS.gene69456:CDS |
| CGCTCGCAGCATCTCAAACC+CGG | 0.501413 | 3.3:+92597572 | MS.gene69456:CDS |
| TGTGCATGTAGACTTGTAGT+AGG | 0.506063 | 3.3:-92605779 | None:intergenic |
| ACGCCTGGTATCAGTGATCC+TGG | 0.507533 | 3.3:+92598081 | MS.gene69456:CDS |
| AAGTTGCTTTGGAATGGAAA+AGG | 0.508393 | 3.3:-92597373 | None:intergenic |
| CATGGCTTCAGCAGAGCAAA+CGG | 0.510069 | 3.3:+92598447 | MS.gene69456:CDS |
| CTCCGCTGATGTTATACTAT+CGG | 0.517324 | 3.3:+92597831 | MS.gene69456:CDS |
| AGTGAGATGGAGTTCGGAGG+TGG | 0.517915 | 3.3:-92597421 | None:intergenic |
| TTCCCTTTGTGTAGACCAGA+AGG | 0.518900 | 3.3:-92605685 | None:intergenic |
| GAAGAAGAATACAGTGGAAG+AGG | 0.520210 | 3.3:-92597351 | None:intergenic |
| CAGTGATCCTGGTATGGAGT+TGG | 0.526144 | 3.3:+92598092 | MS.gene69456:CDS |
| CAAATTCAATGAATCACAAA+GGG | 0.528109 | 3.3:+92597996 | MS.gene69456:CDS |
| TCTGCCATGATTCGAAGATA+GGG | 0.528383 | 3.3:-92603783 | None:intergenic |
| GATACCAAAGCACATGGACC+TGG | 0.530974 | 3.3:-92598243 | None:intergenic |
| GCTTTCTCAGTTTCTTGAAG+AGG | 0.533813 | 3.3:+92598501 | MS.gene69456:CDS |
| GAGTTCGGAGGTGGAGCAGA+AGG | 0.534037 | 3.3:-92597412 | None:intergenic |
| TGTTGCTTCGGCGATTACCC+TGG | 0.538601 | 3.3:+92597307 | MS.gene69456:CDS |
| TTCTCAAGCTCAATGTCCGA+TGG | 0.538928 | 3.3:-92599908 | None:intergenic |
| TTCTAAAGTTGAACCTCCAT+CGG | 0.541066 | 3.3:+92599892 | MS.gene69456:CDS |
| AAACCCGGTCTTTATCTCGT+TGG | 0.542040 | 3.3:+92597587 | MS.gene69456:CDS |
| AAAGGGAACAACTTGTGCTC+AGG | 0.542988 | 3.3:+92598013 | MS.gene69456:CDS |
| TGGTATCAGTGATCCTGGTA+TGG | 0.543755 | 3.3:+92598086 | MS.gene69456:CDS |
| GATGTCTTCAAGATTTCCGA+TGG | 0.546308 | 3.3:-92597615 | None:intergenic |
| AACTTACCATATTGATTGAA+GGG | 0.547874 | 3.3:+92599861 | MS.gene69456:CDS |
| GCTTCGGCGATTACCCTGGA+TGG | 0.551601 | 3.3:+92597311 | MS.gene69456:CDS |
| GTACTTACCAACTCCATACC+AGG | 0.553636 | 3.3:-92598099 | None:intergenic |
| TGTTCCAACGAGATAAAGAC+CGG | 0.559367 | 3.3:-92597591 | None:intergenic |
| TTTCCTTCTGGTCTACACAA+AGG | 0.560574 | 3.3:+92605682 | MS.gene69456:CDS |
| ACGGATGTGATCATCCAAAT+GGG | 0.562981 | 3.3:-92605650 | None:intergenic |
| ATGCTGAGTGAGATGGAGTT+CGG | 0.563325 | 3.3:-92597427 | None:intergenic |
| TATCTCGTTGGAACACCCAT+CGG | 0.564506 | 3.3:+92597599 | MS.gene69456:CDS |
| AATCAGCATCAATGCCAGCT+AGG | 0.568446 | 3.3:-92605726 | None:intergenic |
| TCTGACGGTTCTGATTAGTC+AGG | 0.569034 | 3.3:+92603815 | MS.gene69456:CDS |
| GGCAGAATCTCAGGATCTGA+CGG | 0.585094 | 3.3:+92603800 | MS.gene69456:CDS |
| GGGAACAACTTGTGCTCAGG+AGG | 0.587717 | 3.3:+92598016 | MS.gene69456:CDS |
| AGAAACTGAGAAAGCTTGTG+TGG | 0.598616 | 3.3:-92598493 | None:intergenic |
| ACAAATTCAATGAATCACAA+AGG | 0.598902 | 3.3:+92597995 | MS.gene69456:CDS |
| GAAAAGGAAGAAGAATACAG+TGG | 0.600108 | 3.3:-92597357 | None:intergenic |
| GCAGTGGAAAACGCCATCCA+GGG | 0.611925 | 3.3:-92597324 | None:intergenic |
| AATGACAAAGTTTCACGAAG+AGG | 0.615304 | 3.3:+92598909 | MS.gene69456:CDS |
| AGGAAGAGATTAAGACACGA+GGG | 0.624125 | 3.3:-92605759 | None:intergenic |
| CTGCCATGATTCGAAGATAG+GGG | 0.626829 | 3.3:-92603782 | None:intergenic |
| ACCAAATGTAAATTCATCAG+TGG | 0.627138 | 3.3:-92598289 | None:intergenic |
| TATCTGGATGCTGAGTGAGA+TGG | 0.629940 | 3.3:-92597434 | None:intergenic |
| GTGGCACTTATAAGTGACGC+TGG | 0.634936 | 3.3:+92598057 | MS.gene69456:CDS |
| ATGTCTTCAAGATTTCCGAT+GGG | 0.635435 | 3.3:-92597614 | None:intergenic |
| ATACCAAAGCACATGGACCT+GGG | 0.636160 | 3.3:-92598242 | None:intergenic |
| ATACCAGGATCACTGATACC+AGG | 0.638129 | 3.3:-92598084 | None:intergenic |
| TCAAGCTCAATGTCCGATGG+AGG | 0.640534 | 3.3:-92599905 | None:intergenic |
| TTCCGATAGTATAACATCAG+CGG | 0.642678 | 3.3:-92597833 | None:intergenic |
| AACTGAGAAAGCTTGTGTGG+AGG | 0.643791 | 3.3:-92598490 | None:intergenic |
| GTTGAAACAAGGTGAGATTG+TGG | 0.644732 | 3.3:+92598038 | MS.gene69456:CDS |
| AGAATACAGTGGAAGAGGTG+CGG | 0.646714 | 3.3:-92597346 | None:intergenic |
| AAATGGCGAGAGTCTTTCCA+CGG | 0.651793 | 3.3:+92599952 | MS.gene69456:CDS |
| ATGCAGGCCAAATTATGTGT+TGG | 0.654583 | 3.3:+92598195 | MS.gene69456:intron |
| ATTGGTAGCTGAAAGAACAT+CGG | 0.668003 | 3.3:+92600805 | MS.gene69456:CDS |
| GGTGGAGCAGAAGGATAAAG+GGG | 0.669561 | 3.3:-92597403 | None:intergenic |
| GTGCTCAGGAGGTTGAAACA+AGG | 0.672276 | 3.3:+92598027 | MS.gene69456:CDS |
| TTCATCAGTGGATAAACCCG+AGG | 0.672572 | 3.3:-92598277 | None:intergenic |
| CATCAGCGGAGTTCAACACC+CGG | 0.673155 | 3.3:-92597819 | None:intergenic |
| AGAGCCGATACCAAAGCACA+TGG | 0.677490 | 3.3:-92598249 | None:intergenic |
| TTATACTATCGGAAGACACG+AGG | 0.685377 | 3.3:+92597842 | MS.gene69456:CDS |
| TAGGAAGAGATTAAGACACG+AGG | 0.690399 | 3.3:-92605760 | None:intergenic |
| GTTCCAACGAGATAAAGACC+GGG | 0.703315 | 3.3:-92597590 | None:intergenic |
| CTGAGTGAGATGGAGTTCGG+AGG | 0.718535 | 3.3:-92597424 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 92597306 | 92605807 | 92597306 | ID=MS.gene69456 |
| chr3.3 | mRNA | 92597306 | 92605807 | 92597306 | ID=MS.gene69456.t1;Parent=MS.gene69456 |
| chr3.3 | exon | 92597306 | 92597467 | 92597306 | ID=MS.gene69456.t1.exon1;Parent=MS.gene69456.t1 |
| chr3.3 | CDS | 92597306 | 92597467 | 92597306 | ID=cds.MS.gene69456.t1;Parent=MS.gene69456.t1 |
| chr3.3 | exon | 92597551 | 92597645 | 92597551 | ID=MS.gene69456.t1.exon2;Parent=MS.gene69456.t1 |
| chr3.3 | CDS | 92597551 | 92597645 | 92597551 | ID=cds.MS.gene69456.t1;Parent=MS.gene69456.t1 |
| chr3.3 | exon | 92597813 | 92597909 | 92597813 | ID=MS.gene69456.t1.exon3;Parent=MS.gene69456.t1 |
| chr3.3 | CDS | 92597813 | 92597909 | 92597813 | ID=cds.MS.gene69456.t1;Parent=MS.gene69456.t1 |
| chr3.3 | exon | 92597985 | 92598113 | 92597985 | ID=MS.gene69456.t1.exon4;Parent=MS.gene69456.t1 |
| chr3.3 | CDS | 92597985 | 92598113 | 92597985 | ID=cds.MS.gene69456.t1;Parent=MS.gene69456.t1 |
| chr3.3 | exon | 92598201 | 92598309 | 92598201 | ID=MS.gene69456.t1.exon5;Parent=MS.gene69456.t1 |
| chr3.3 | CDS | 92598201 | 92598309 | 92598201 | ID=cds.MS.gene69456.t1;Parent=MS.gene69456.t1 |
| chr3.3 | exon | 92598404 | 92598548 | 92598404 | ID=MS.gene69456.t1.exon6;Parent=MS.gene69456.t1 |
| chr3.3 | CDS | 92598404 | 92598548 | 92598404 | ID=cds.MS.gene69456.t1;Parent=MS.gene69456.t1 |
| chr3.3 | exon | 92598888 | 92598930 | 92598888 | ID=MS.gene69456.t1.exon7;Parent=MS.gene69456.t1 |
| chr3.3 | CDS | 92598888 | 92598930 | 92598888 | ID=cds.MS.gene69456.t1;Parent=MS.gene69456.t1 |
| chr3.3 | exon | 92599800 | 92599973 | 92599800 | ID=MS.gene69456.t1.exon8;Parent=MS.gene69456.t1 |
| chr3.3 | CDS | 92599800 | 92599973 | 92599800 | ID=cds.MS.gene69456.t1;Parent=MS.gene69456.t1 |
| chr3.3 | exon | 92600797 | 92600826 | 92600797 | ID=MS.gene69456.t1.exon9;Parent=MS.gene69456.t1 |
| chr3.3 | CDS | 92600797 | 92600826 | 92600797 | ID=cds.MS.gene69456.t1;Parent=MS.gene69456.t1 |
| chr3.3 | exon | 92603771 | 92603836 | 92603771 | ID=MS.gene69456.t1.exon10;Parent=MS.gene69456.t1 |
| chr3.3 | CDS | 92603771 | 92603836 | 92603771 | ID=cds.MS.gene69456.t1;Parent=MS.gene69456.t1 |
| chr3.3 | exon | 92605610 | 92605807 | 92605610 | ID=MS.gene69456.t1.exon11;Parent=MS.gene69456.t1 |
| chr3.3 | CDS | 92605610 | 92605807 | 92605610 | ID=cds.MS.gene69456.t1;Parent=MS.gene69456.t1 |
| Gene Sequence |
| Protein sequence |
