AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71139

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71139.t1 MTR_2g060350 96.536 433 15 0 1 433 12 444 0.0 882
MS.gene71139.t1 MTR_3g030540 60.880 432 160 5 3 432 13 437 0.0 519
MS.gene71139.t1 MTR_1g052315 53.810 420 182 7 17 430 17 430 3.17e-150 434
MS.gene71139.t1 MTR_7g091850 50.794 441 201 7 2 433 13 446 2.05e-138 404
MS.gene71139.t1 MTR_7g091840 54.799 323 138 5 57 374 59 378 7.02e-119 352
MS.gene71139.t1 MTR_2g103410 46.631 371 191 5 62 428 58 425 4.90e-114 342
MS.gene71139.t1 MTR_8g065030 40.093 429 235 8 4 428 19 429 2.85e-109 330
MS.gene71139.t1 MTR_6g005630 43.732 343 180 7 65 404 47 379 7.19e-93 286
MS.gene71139.t1 MTR_4g094715 43.251 363 191 7 57 408 59 417 1.35e-90 282
MS.gene71139.t1 MTR_7g110050 40.872 367 207 6 51 408 55 420 3.28e-90 281
MS.gene71139.t1 MTR_3g084360 40.650 369 200 7 59 419 20 377 1.29e-87 272
MS.gene71139.t1 MTR_5g033680 38.587 368 217 7 68 428 64 429 2.88e-87 274
MS.gene71139.t1 MTR_2g049400 41.885 382 204 10 54 429 19 388 6.23e-87 270
MS.gene71139.t1 MTR_1g086390 40.872 367 192 9 59 408 77 435 1.62e-86 272
MS.gene71139.t1 MTR_8g065010 38.360 378 196 7 59 405 103 474 1.39e-84 268
MS.gene71139.t1 MTR_4g084050 38.400 375 203 8 60 419 20 381 1.72e-84 264
MS.gene71139.t1 MTR_5g043350 40.556 360 196 8 68 419 34 383 8.86e-83 260
MS.gene71139.t1 MTR_3g084380 39.776 357 194 7 68 415 26 370 9.33e-82 257
MS.gene71139.t1 MTR_7g113910 49.425 261 111 3 155 415 95 334 4.85e-81 254
MS.gene71139.t1 MTR_3g054320 38.333 360 195 10 68 417 35 377 1.86e-80 254
MS.gene71139.t1 MTR_5g034090 38.275 371 193 8 65 405 123 487 3.53e-80 257
MS.gene71139.t1 MTR_6g005630 41.399 343 165 10 65 404 47 356 6.36e-80 253
MS.gene71139.t1 MTR_6g028080 38.692 367 205 8 64 421 31 386 8.78e-79 249
MS.gene71139.t1 MTR_2g103650 40.233 343 197 5 65 401 30 370 1.17e-78 249
MS.gene71139.t1 MTR_3g438050 41.484 364 191 12 57 408 17 370 4.99e-77 245
MS.gene71139.t1 MTR_4g120320 37.221 403 231 14 40 432 32 422 5.96e-76 243
MS.gene71139.t1 MTR_2g032710 39.669 363 204 7 57 408 61 419 6.79e-76 244
MS.gene71139.t1 MTR_8g010080 39.353 371 199 9 59 423 27 377 4.23e-75 239
MS.gene71139.t1 MTR_2g435640 35.385 390 227 12 51 428 43 419 1.15e-74 239
MS.gene71139.t1 MTR_8g010110 39.130 368 199 9 65 426 33 381 2.08e-74 238
MS.gene71139.t1 MTR_8g030930 40.000 355 196 9 62 407 26 372 2.37e-74 238
MS.gene71139.t1 MTR_8g010070 38.542 384 205 10 47 423 20 379 3.46e-74 237
MS.gene71139.t1 MTR_8g010090 38.859 368 200 9 65 426 33 381 8.36e-74 236
MS.gene71139.t1 MTR_2g103410 46.008 263 135 5 62 320 58 317 1.39e-73 234
MS.gene71139.t1 MTR_4g083660 39.437 355 200 10 55 403 1 346 1.40e-72 233
MS.gene71139.t1 MTR_8g009810 38.005 371 216 9 63 428 35 396 8.09e-72 231
MS.gene71139.t1 MTR_0157s0060 37.119 361 204 6 67 419 2 347 1.72e-69 224
MS.gene71139.t1 MTR_4g034020 37.119 361 204 6 67 419 2 347 1.72e-69 224
MS.gene71139.t1 MTR_2g058670 34.908 381 237 9 55 430 17 391 1.59e-68 224
MS.gene71139.t1 MTR_8g008765 37.092 337 189 6 59 390 27 345 5.96e-68 220
MS.gene71139.t1 MTR_2g058840 34.908 381 235 10 55 430 17 389 7.05e-68 222
MS.gene71139.t1 MTR_7g035415 40.753 292 153 6 65 349 42 320 8.46e-68 219
MS.gene71139.t1 MTR_2g029170 37.537 341 198 9 93 429 29 358 3.40e-65 213
MS.gene71139.t1 MTR_2g058640 35.789 380 221 9 62 430 24 391 1.66e-64 213
MS.gene71139.t1 MTR_8g010040 36.212 359 203 9 65 417 34 372 4.19e-64 211
MS.gene71139.t1 MTR_1g088160 32.911 395 243 11 46 428 18 402 1.22e-63 211
MS.gene71139.t1 MTR_1g012390 34.677 372 226 9 55 419 20 381 6.43e-62 206
MS.gene71139.t1 MTR_1g013740 33.430 344 201 7 63 401 35 355 1.89e-58 196
MS.gene71139.t1 MTR_5g097860 35.185 324 197 8 62 379 24 340 2.76e-58 194
MS.gene71139.t1 MTR_8g077320 37.829 304 140 6 62 349 32 302 3.44e-58 195
MS.gene71139.t1 MTR_1g013730 34.302 344 198 9 63 401 27 347 1.48e-57 194
MS.gene71139.t1 MTR_5g063430 36.545 301 173 8 62 356 24 312 1.76e-57 192
MS.gene71139.t1 MTR_5g034090 37.762 286 144 6 65 320 123 404 2.18e-57 194
MS.gene71139.t1 MTR_8g010050 33.247 388 199 9 43 423 11 345 3.94e-57 192
MS.gene71139.t1 MTR_8g008700 38.148 270 147 4 120 387 14 265 1.60e-54 183
MS.gene71139.t1 MTR_8g031180 39.269 219 123 3 190 403 59 272 6.00e-46 160
MS.gene71139.t1 MTR_2g100040 32.578 353 178 10 56 401 17 316 1.08e-43 157
MS.gene71139.t1 MTR_4g120730 24.444 405 229 11 63 418 40 416 2.70e-22 99.0
MS.gene71139.t1 MTR_2g042740 28.958 259 159 7 157 401 169 416 7.31e-21 95.1
MS.gene71139.t1 MTR_3g449590 25.840 387 229 14 63 410 47 414 1.34e-20 94.0
MS.gene71139.t1 MTR_5g090160 24.802 379 240 13 63 408 44 410 1.03e-18 88.6
MS.gene71139.t1 MTR_7g110710 26.158 367 201 13 63 383 43 385 1.29e-18 88.2
MS.gene71139.t1 MTR_4g124980 26.255 259 166 7 157 401 187 434 1.31e-18 88.2
MS.gene71139.t1 MTR_7g110710 26.158 367 201 13 63 383 43 385 2.52e-18 87.4
MS.gene71139.t1 MTR_1g086030 26.891 357 194 13 63 375 82 415 3.04e-18 87.4
MS.gene71139.t1 MTR_1g086030 29.870 231 140 5 157 375 91 311 5.78e-18 85.9
MS.gene71139.t1 MTR_2g435340 22.126 348 222 7 108 418 13 348 7.79e-18 85.1
MS.gene71139.t1 MTR_2g435340 22.222 405 238 10 63 418 37 413 1.13e-16 82.0
MS.gene71139.t1 MTR_7g088550 23.077 403 250 15 43 404 30 413 2.50e-16 81.3
MS.gene71139.t1 MTR_3g096330 25.794 252 163 6 146 383 177 418 7.72e-16 80.5
MS.gene71139.t1 MTR_8g098840 25.633 316 177 11 64 339 45 342 7.91e-16 79.7
MS.gene71139.t1 MTR_8g028725 24.148 352 219 13 63 379 55 393 2.77e-15 78.2
MS.gene71139.t1 MTR_1g100060 22.487 378 238 11 65 404 95 455 4.29e-14 74.3
MS.gene71139.t1 MTR_7g078680 22.573 412 251 13 64 430 56 444 5.25e-14 73.9
MS.gene71139.t1 MTR_1g100050 22.487 378 238 14 65 404 52 412 1.10e-12 70.1
MS.gene71139.t1 MTR_1g100040 22.102 371 248 13 65 404 58 418 1.68e-11 66.2
MS.gene71139.t1 MTR_1g099850 22.642 371 246 14 65 404 57 417 1.88e-11 66.2
MS.gene71139.t1 MTR_7g092470 23.827 277 185 5 136 396 123 389 7.02e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71139.t1 AT3G07970 56.608 401 161 8 39 431 44 439 4.22e-150 433
MS.gene71139.t1 AT3G57510 48.636 440 198 12 2 431 10 431 8.76e-138 402
MS.gene71139.t1 AT2G41850 50.000 438 194 12 1 430 10 430 4.46e-127 375
MS.gene71139.t1 AT1G80170 47.340 376 190 6 58 428 48 420 1.68e-117 350
MS.gene71139.t1 AT1G23460 45.383 379 198 7 55 428 33 407 4.69e-109 328
MS.gene71139.t1 AT1G23460 45.383 379 198 7 55 428 57 431 5.91e-109 329
MS.gene71139.t1 AT1G70500 43.536 379 205 7 55 428 57 431 7.93e-105 319
MS.gene71139.t1 AT1G56710 43.417 357 185 7 65 408 50 402 2.40e-92 286
MS.gene71139.t1 AT3G15720 42.703 370 190 8 59 419 20 376 7.65e-92 285
MS.gene71139.t1 AT3G15720 42.703 370 190 8 59 419 19 375 8.73e-92 285
MS.gene71139.t1 AT5G14650 40.984 366 201 7 48 408 36 391 1.60e-90 281
MS.gene71139.t1 AT3G15720 42.432 370 188 9 59 419 19 372 4.19e-89 278
MS.gene71139.t1 AT1G48100 40.054 367 197 8 57 408 74 432 2.84e-88 276
MS.gene71139.t1 AT2G43890 39.523 377 216 8 56 429 22 389 5.19e-87 271
MS.gene71139.t1 AT4G35670 41.111 360 195 7 65 418 26 374 5.41e-86 268
MS.gene71139.t1 AT3G26610 37.371 388 233 7 26 408 31 413 5.64e-86 270
MS.gene71139.t1 AT5G27530 39.944 358 190 5 68 419 58 396 7.80e-85 267
MS.gene71139.t1 AT1G05650 39.628 376 216 7 56 428 21 388 2.84e-84 264
MS.gene71139.t1 AT3G15720 43.034 323 165 6 103 419 3 312 7.42e-83 260
MS.gene71139.t1 AT1G02460 40.761 368 190 9 61 408 87 446 8.57e-83 263
MS.gene71139.t1 AT1G05660 39.946 368 208 8 65 428 30 388 1.52e-82 259
MS.gene71139.t1 AT1G60590 38.649 370 196 7 65 405 125 492 1.54e-82 264
MS.gene71139.t1 AT1G10640 38.420 367 198 7 65 405 121 485 3.07e-81 260
MS.gene71139.t1 AT1G80140 41.195 318 169 7 97 408 6 311 5.79e-81 253
MS.gene71139.t1 AT4G18180 37.831 378 209 8 62 419 38 409 5.39e-79 251
MS.gene71139.t1 AT5G44840 37.222 360 210 7 65 418 26 375 7.29e-78 247
MS.gene71139.t1 AT1G80170 46.442 267 136 5 58 320 48 311 1.59e-77 244
MS.gene71139.t1 AT2G33160 39.628 376 211 9 62 428 31 399 1.05e-76 251
MS.gene71139.t1 AT2G43870 39.426 383 215 12 54 430 12 383 1.07e-76 244
MS.gene71139.t1 AT4G01890 38.268 358 201 8 65 406 67 420 2.87e-76 245
MS.gene71139.t1 AT5G17200 38.660 388 197 9 59 419 35 408 8.36e-76 243
MS.gene71139.t1 AT2G15460 36.148 379 223 9 62 429 30 400 4.47e-75 240
MS.gene71139.t1 AT3G59850 40.351 342 189 10 68 403 28 360 6.73e-75 239
MS.gene71139.t1 AT1G02790 37.598 383 206 11 65 430 54 420 1.36e-74 239
MS.gene71139.t1 AT3G59850 40.000 345 192 10 65 403 69 404 1.38e-74 240
MS.gene71139.t1 AT1G43080 37.368 380 217 11 62 429 30 400 2.28e-74 238
MS.gene71139.t1 AT2G15450 35.979 378 223 9 62 428 30 399 3.01e-74 238
MS.gene71139.t1 AT2G15470 35.884 379 224 9 62 429 30 400 1.06e-73 236
MS.gene71139.t1 AT2G26620 36.605 377 222 9 62 429 30 398 1.43e-72 234
MS.gene71139.t1 AT1G43090 36.800 375 218 9 62 425 30 396 1.77e-72 234
MS.gene71139.t1 AT2G43880 39.093 353 200 8 56 403 24 366 3.91e-72 232
MS.gene71139.t1 AT1G65570 35.411 401 233 10 35 429 14 394 6.17e-72 232
MS.gene71139.t1 AT2G40310 36.605 377 218 11 65 429 33 400 6.65e-72 232
MS.gene71139.t1 AT1G17150 37.736 371 216 9 65 428 35 397 8.31e-72 232
MS.gene71139.t1 AT2G43860 39.833 359 200 10 53 403 20 370 1.64e-71 231
MS.gene71139.t1 AT1G43100 36.533 375 219 9 62 425 30 396 2.61e-71 231
MS.gene71139.t1 AT4G13760 35.356 379 226 9 62 429 30 400 3.79e-71 230
MS.gene71139.t1 AT3G07820 36.264 364 217 8 72 429 30 384 3.81e-71 229
MS.gene71139.t1 AT5G48140 37.892 351 202 8 74 418 33 373 5.74e-71 229
MS.gene71139.t1 AT4G35670 42.238 277 149 2 142 418 2 267 8.61e-71 225
MS.gene71139.t1 AT1G78400 36.828 372 218 11 65 428 37 399 1.42e-70 228
MS.gene71139.t1 AT3G07830 35.165 364 221 8 72 429 31 385 4.97e-70 227
MS.gene71139.t1 AT3G07840 35.580 371 216 9 62 418 13 374 1.01e-69 226
MS.gene71139.t1 AT3G14040 37.534 373 217 9 63 428 71 434 1.20e-68 225
MS.gene71139.t1 AT3G07850 37.203 379 222 9 57 428 64 433 2.54e-68 224
MS.gene71139.t1 AT5G44830 36.503 326 185 4 95 418 11 316 1.23e-67 219
MS.gene71139.t1 AT4G32380 34.677 372 223 8 62 423 25 386 5.85e-67 221
MS.gene71139.t1 AT5G14650 38.849 278 157 5 48 320 36 305 1.49e-61 202
MS.gene71139.t1 AT4G32370 37.908 306 168 7 58 351 29 324 3.30e-61 202
MS.gene71139.t1 AT2G43880 38.835 309 174 8 56 359 24 322 8.31e-59 196
MS.gene71139.t1 AT4G32375 39.080 261 148 3 142 401 13 263 1.21e-58 198
MS.gene71139.t1 AT5G39910 34.754 305 168 7 62 358 35 316 5.93e-52 179
MS.gene71139.t1 AT4G32380 30.811 370 199 8 62 423 25 345 6.05e-52 180
MS.gene71139.t1 AT1G19170 28.185 259 161 6 157 401 201 448 2.76e-21 96.7
MS.gene71139.t1 AT3G62110 29.310 232 141 5 157 375 156 377 4.57e-21 95.5
MS.gene71139.t1 AT3G62110 29.310 232 141 5 157 375 156 377 4.57e-21 95.5
MS.gene71139.t1 AT3G42950 27.005 374 227 14 62 401 65 426 1.00e-20 94.7
MS.gene71139.t1 AT4G23820 24.810 395 239 14 63 418 41 416 9.47e-20 91.7
MS.gene71139.t1 AT4G33440 25.000 392 218 14 62 397 71 442 4.48e-18 86.7
MS.gene71139.t1 AT3G16850 24.151 265 169 5 157 404 153 402 9.11e-18 85.5
MS.gene71139.t1 AT3G06770 24.409 381 225 12 63 401 44 403 9.36e-18 85.5
MS.gene71139.t1 AT3G06770 24.409 381 225 12 63 401 43 402 1.05e-17 85.5
MS.gene71139.t1 AT5G41870 25.141 354 207 14 62 375 44 379 3.30e-17 84.0
MS.gene71139.t1 AT2G23900 24.684 316 179 10 65 339 58 355 4.18e-17 83.6
MS.gene71139.t1 AT5G49215 23.760 383 229 11 63 403 41 402 4.49e-17 83.6
MS.gene71139.t1 AT5G49215 23.760 383 229 11 63 403 43 404 4.66e-17 83.6
MS.gene71139.t1 AT3G48950 24.843 318 176 10 65 339 47 344 9.02e-17 82.8
MS.gene71139.t1 AT4G23500 25.316 316 178 11 64 339 69 366 3.76e-16 80.9
MS.gene71139.t1 AT3G06770 24.419 258 171 5 157 401 69 315 9.32e-16 79.0
MS.gene71139.t1 AT3G06770 24.419 258 171 5 157 401 87 333 9.53e-16 79.0
MS.gene71139.t1 AT3G06770 24.419 258 171 5 157 401 87 333 9.53e-16 79.0
MS.gene71139.t1 AT3G61490 30.208 192 113 6 157 335 173 356 3.37e-14 74.7
MS.gene71139.t1 AT3G61490 30.208 192 113 6 157 335 271 454 3.66e-14 75.1
MS.gene71139.t1 AT3G61490 30.208 192 113 6 157 335 158 341 4.40e-14 74.3
MS.gene71139.t1 AT3G61490 30.208 192 113 6 157 335 158 341 4.40e-14 74.3
MS.gene71139.t1 AT3G61490 30.208 192 113 6 157 335 158 341 4.40e-14 74.3

Find 86 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACCAAATACTGCCTTTA+TGG 0.149151 4.1:-56691951 MS.gene71139:intron
GACATAATAAACTGTGATAT+TGG 0.217261 4.1:-56690916 MS.gene71139:CDS
CTCACCATAAAGGCAGTATT+TGG 0.245870 4.1:+56691947 None:intergenic
TCGAATTCATGTTAGCTCTA+TGG 0.262220 4.1:-56691606 MS.gene71139:intron
AAAACAAAACATCAACATTT+TGG 0.265438 4.1:-56692305 MS.gene71139:CDS
GTAGTTCCTCCACCACCTTC+TGG 0.295016 4.1:+56689379 None:intergenic
ATTTGAAAGTAAAGAATCTA+AGG 0.330656 4.1:-56691071 MS.gene71139:CDS
GAGATGGTATATCCTATTCT+TGG 0.332828 4.1:+56692000 None:intergenic
TTGCAGAGAAATATACTTGC+AGG 0.334080 4.1:-56689416 MS.gene71139:CDS
GCACGGCTTTGTTCCGCTCT+TGG 0.338883 4.1:+56689524 None:intergenic
GTAGTATTGATCTATGATTA+TGG 0.353266 4.1:+56689567 None:intergenic
GTGAACAGGGCTGCCTTAAC+AGG 0.368388 4.1:-56690175 MS.gene71139:CDS
GCTCTTGGCATGGCTCTGTT+TGG 0.383793 4.1:+56689539 None:intergenic
GCGGACCCGGACATGGAATC+AGG 0.384459 4.1:-56690706 MS.gene71139:intron
GTCTCGAATTTCAAAGTTGA+TGG 0.389296 4.1:-56691513 MS.gene71139:CDS
AAAACTTACAAGGTTATTGT+TGG 0.389616 4.1:+56691434 None:intergenic
TCCATTAGTTGTTCCTGTTA+AGG 0.393409 4.1:+56690162 None:intergenic
ACATAATAAACTGTGATATT+GGG 0.395546 4.1:-56690915 MS.gene71139:CDS
TTTCCAATGTAGTAGTGAAC+AGG 0.405039 4.1:-56690189 MS.gene71139:CDS
AGAGAAAAGTGTATCTCTAT+TGG 0.419321 4.1:+56692268 None:intergenic
ATATGATCACAGGGTGGTTC+AGG 0.430537 4.1:-56689643 MS.gene71139:intron
ATGAAACCTGATTCCATGTC+CGG 0.431003 4.1:+56690700 None:intergenic
GCCTTAACAGGAACAACTAA+TGG 0.431469 4.1:-56690163 MS.gene71139:CDS
TGTCAGCATATGAAGAAGAT+AGG 0.435363 4.1:-56691560 MS.gene71139:CDS
TATTATCTTACAGTTGGTCT+TGG 0.440978 4.1:+56691197 None:intergenic
GATTGTATTTCGATAGTTAG+TGG 0.441436 4.1:-56690761 MS.gene71139:CDS
TTCTGCAGTATTGGAAGCTT+AGG 0.444981 4.1:-56690235 MS.gene71139:intron
TAGTATTGATCTATGATTAT+GGG 0.445220 4.1:+56689568 None:intergenic
TCCTCAATGCTCTCCACAAT+AGG 0.452988 4.1:-56689311 None:intergenic
GATGGTGGTGGCACTTTCAA+TGG 0.457262 4.1:-56691495 MS.gene71139:CDS
AGATGGTATATCCTATTCTT+GGG 0.459795 4.1:+56692001 None:intergenic
TAGTGTAAGACTCACCATAA+AGG 0.459802 4.1:+56691937 None:intergenic
TCTCCTTAAGGCATTTAAGA+AGG 0.463765 4.1:-56692065 MS.gene71139:intron
CACATGAATTCCATCGGTGT+TGG 0.469737 4.1:+56690957 None:intergenic
GCTTTGTTCCGCTCTTGGCA+TGG 0.478055 4.1:+56689529 None:intergenic
CAAAACTTGGCTTAATTGCA+CGG 0.479353 4.1:+56689507 None:intergenic
ACATGAATTCCATCGGTGTT+GGG 0.481545 4.1:+56690958 None:intergenic
TGCATATGAGTAATGAAGAT+AGG 0.483118 4.1:+56692405 None:intergenic
ACAATATCCATTGCCTATTG+TGG 0.484295 4.1:+56689298 None:intergenic
TGGAATGAAGCATGTTCTAA+AGG 0.492280 4.1:-56692040 MS.gene71139:CDS
AGCCCTGTTCACTACTACAT+TGG 0.494296 4.1:+56690186 None:intergenic
CTCTTCAAAGTCTCAAGGAA+TGG 0.495111 4.1:-56692249 MS.gene71139:CDS
GGAATGAAGCATGTTCTAAA+GGG 0.497809 4.1:-56692039 MS.gene71139:CDS
ATGGTTAGTGTTGATGACTT+TGG 0.500293 4.1:-56692230 MS.gene71139:CDS
AGATGGAAGAGATGATAGAG+AGG 0.500756 4.1:-56692198 MS.gene71139:intron
GACTTTGGAGCAAGAGCAGA+TGG 0.502754 4.1:-56692215 MS.gene71139:CDS
TCCTTGTGGTGCCCAAGAAT+AGG 0.505871 4.1:-56692012 MS.gene71139:CDS
TCGGTGTTGGGACTATCCTC+TGG 0.506883 4.1:+56690970 None:intergenic
AATTTCAAAGTTGATGGTGG+TGG 0.508006 4.1:-56691507 MS.gene71139:CDS
TTTCTCTGCAAGGAACGCTT+CGG 0.508888 4.1:+56689428 None:intergenic
ATAGATATCACTTGCGGACC+CGG 0.513667 4.1:-56690719 MS.gene71139:CDS
TCAACATTGGCTTGATGAAG+TGG 0.522002 4.1:+56692362 None:intergenic
TTAAGGCATTTAAGAAGGCA+TGG 0.527618 4.1:-56692060 MS.gene71139:intron
GTGGCACTTTCAATGGCAAT+GGG 0.535626 4.1:-56691488 MS.gene71139:CDS
ATCACTTGCGGACCCGGACA+TGG 0.542140 4.1:-56690713 MS.gene71139:CDS
GGTGGCACTTTCAATGGCAA+TGG 0.545089 4.1:-56691489 MS.gene71139:CDS
GGTCTTGGTTCATCATTGCA+TGG 0.546282 4.1:+56691212 None:intergenic
ATGCAATGGCTCTTACATAG+TGG 0.549275 4.1:-56692387 MS.gene71139:CDS
ATAAACTGTGATATTGGGAC+AGG 0.552284 4.1:-56690910 MS.gene71139:intron
TCAATCTACCATCATGTACA+TGG 0.568484 4.1:+56692330 None:intergenic
AATGTTAGATATGTAAATAG+AGG 0.571277 4.1:-56689343 MS.gene71139:CDS
TTCAGCCACATGAATTCCAT+CGG 0.571554 4.1:+56690951 None:intergenic
TTCTAAAGGGTCTATCCTTG+TGG 0.572564 4.1:-56692026 MS.gene71139:CDS
ATGAAGAAGATAGGCAACAC+TGG 0.573069 4.1:-56691551 MS.gene71139:CDS
TGAAACCTGATTCCATGTCC+GGG 0.576277 4.1:+56690701 None:intergenic
CTAGAACCAGAAGGTGGTGG+AGG 0.582001 4.1:-56689385 MS.gene71139:CDS
GCCTATTGTGGAGAGCATTG+AGG 0.588158 4.1:+56689310 None:intergenic
TGCAAGTATATTTCTCTGCA+AGG 0.588447 4.1:+56689418 None:intergenic
GAACATAGAAATGCAAAATG+TGG 0.591101 4.1:-56689596 MS.gene71139:CDS
ATTCTAGAACCAGAAGGTGG+TGG 0.593437 4.1:-56689388 MS.gene71139:CDS
AAGGCAGTATTTGGTTGACA+TGG 0.594353 4.1:+56691956 None:intergenic
GAGGATAGTCCCAACACCGA+TGG 0.596952 4.1:-56690967 MS.gene71139:CDS
CGTTACTACCATGTACATGA+TGG 0.600317 4.1:-56692338 MS.gene71139:CDS
TCCTATTCTTGGGCACCACA+AGG 0.600648 4.1:+56692011 None:intergenic
TTAGCTCTATGGAACAATTG+AGG 0.602214 4.1:-56691595 MS.gene71139:intron
ATGGTAGTAACGTTCAACAT+TGG 0.605356 4.1:+56692349 None:intergenic
TTTGATTGTGAGAGCGCCAG+AGG 0.607314 4.1:-56690986 MS.gene71139:CDS
AAACAGAGCCATGCCAAGAG+CGG 0.620744 4.1:-56689537 MS.gene71139:CDS
TCTTCATTACTCATATGCAA+TGG 0.629492 4.1:-56692401 MS.gene71139:CDS
TCTATGGAACAATTGAGGCA+TGG 0.634765 4.1:-56691590 MS.gene71139:CDS
TCGAATTTCAAAGTTGATGG+TGG 0.648608 4.1:-56691510 MS.gene71139:CDS
CAACACCGATGGAATTCATG+TGG 0.652375 4.1:-56690956 MS.gene71139:CDS
TTCCAATGTAGTAGTGAACA+GGG 0.653388 4.1:-56690188 MS.gene71139:CDS
CGAGCTATAGATATCACTTG+CGG 0.678372 4.1:-56690725 MS.gene71139:CDS
GTGATTCTAGAACCAGAAGG+TGG 0.703740 4.1:-56689391 MS.gene71139:CDS
GATGTGATTCTAGAACCAGA+AGG 0.739033 4.1:-56689394 MS.gene71139:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAACAATAACATATA+TGG - chr4.1:56689552-56689571 MS.gene71139:CDS 10.0%
!! AAAATATAACAAATGAAAAT+TGG + chr4.1:56690335-56690354 None:intergenic 10.0%
!! AATATACTTGTAAAAATTTA+TGG + chr4.1:56690563-56690582 None:intergenic 10.0%
!! ACATTCAAAAAATAAATATT+TGG - chr4.1:56690874-56690893 MS.gene71139:intron 10.0%
!!! AACATATATTTTTTTTTTGA+GGG - chr4.1:56691226-56691245 MS.gene71139:intron 10.0%
!!! TTATTTTAAATTTTCTCTTT+TGG + chr4.1:56691319-56691338 None:intergenic 10.0%
!! AATGGAAAATAAATTTATGT+AGG - chr4.1:56690607-56690626 MS.gene71139:intron 15.0%
!! AATTTCAACATATATGAAAA+AGG - chr4.1:56691253-56691272 MS.gene71139:intron 15.0%
!! ATATAAAAAAATGTCTTAAC+TGG + chr4.1:56691426-56691445 None:intergenic 15.0%
!! ATTTAGAAGAAATTAATAAG+TGG + chr4.1:56691928-56691947 None:intergenic 15.0%
!! ATTTCAACATATATGAAAAA+GGG - chr4.1:56691254-56691273 MS.gene71139:intron 15.0%
!! CTAAATAAAAAACATTCAAT+TGG - chr4.1:56690900-56690919 MS.gene71139:intron 15.0%
!! TTTCTATGGTTATTAATTAA+AGG + chr4.1:56691964-56691983 None:intergenic 15.0%
!!! AAATGAATGATTTCAAATTT+TGG - chr4.1:56690391-56690410 MS.gene71139:intron 15.0%
!!! ATTGATTAAAACTTTTTCTT+TGG - chr4.1:56691113-56691132 MS.gene71139:intron 15.0%
!!! CAACATATATTTTTTTTTTG+AGG - chr4.1:56691225-56691244 MS.gene71139:intron 15.0%
!!! TTTTTTTATATTTGCTTTTC+TGG - chr4.1:56691435-56691454 MS.gene71139:intron 15.0%
!! AATGTTAGATATGTAAATAG+AGG - chr4.1:56692382-56692401 MS.gene71139:CDS 20.0%
!! ACATAATAAACTGTGATATT+GGG - chr4.1:56690810-56690829 MS.gene71139:intron 20.0%
!! ATTTGAAAGTAAAGAATCTA+AGG - chr4.1:56690654-56690673 MS.gene71139:intron 20.0%
!! GAAAATTGGAAGAAAAAAAA+AGG + chr4.1:56690321-56690340 None:intergenic 20.0%
!! TAATATATATTCTGCAGTAT+TGG - chr4.1:56691481-56691500 MS.gene71139:CDS 20.0%
!!! AAAACAAAACATCAACATTT+TGG - chr4.1:56689420-56689439 MS.gene71139:CDS 20.0%
!!! AAAACTTTAAAGTTGAGATT+GGG - chr4.1:56691150-56691169 MS.gene71139:intron 20.0%
!!! ATTAAAACTTTTTCTTTGGA+GGG - chr4.1:56691117-56691136 MS.gene71139:intron 20.0%
!!! ATTTTGAAAATTAGTCTGTT+AGG - chr4.1:56691625-56691644 MS.gene71139:intron 20.0%
!!! TAAAACTTTAAAGTTGAGAT+TGG - chr4.1:56691149-56691168 MS.gene71139:intron 20.0%
!!! TAGTATTGATCTATGATTAT+GGG + chr4.1:56692160-56692179 None:intergenic 20.0%
!!! TCAGTAATGCAATTTTAAAT+TGG + chr4.1:56689836-56689855 None:intergenic 20.0%
!!! TTACTTTTTGAACTAATCTA+AGG + chr4.1:56690015-56690034 None:intergenic 20.0%
!!! TTTACTTTCTTTTTTGTTGA+AGG - chr4.1:56690937-56690956 MS.gene71139:CDS 20.0%
!!! TTTCATTTAATTTCTCCTTA+AGG - chr4.1:56689648-56689667 MS.gene71139:intron 20.0%
! AAAACTTACAAGGTTATTGT+TGG + chr4.1:56690294-56690313 None:intergenic 25.0%
! AGAAGAGGGAAAATAAATTA+AGG + chr4.1:56690481-56690500 None:intergenic 25.0%
! ATAACCGATAAAACAAACAT+TGG - chr4.1:56691787-56691806 MS.gene71139:intron 25.0%
! ATATTGTAATAAAACTCACG+TGG + chr4.1:56692042-56692061 None:intergenic 25.0%
! CTCAATCTATGAATTAAATG+AGG + chr4.1:56691068-56691087 None:intergenic 25.0%
! GAAGAATATTATCTTACAGT+TGG + chr4.1:56690537-56690556 None:intergenic 25.0%
! GACATAATAAACTGTGATAT+TGG - chr4.1:56690809-56690828 MS.gene71139:intron 25.0%
! GGAAAATAAATTTATGTAGG+CGG - chr4.1:56690610-56690629 MS.gene71139:intron 25.0%
! TTATGAAAAATACTTGAGAG+AGG - chr4.1:56690368-56690387 MS.gene71139:intron 25.0%
! TTCAAAAAGTAAAGCTTAGT+GGG - chr4.1:56690023-56690042 MS.gene71139:intron 25.0%
!! AAAAGGTACAAAAACTTACA+AGG + chr4.1:56690304-56690323 None:intergenic 25.0%
!! CTAATCGATTCATACTTTAA+TGG - chr4.1:56690589-56690608 MS.gene71139:intron 25.0%
!! TAAGCTATTTGCCAAATTTT+AGG - chr4.1:56691761-56691780 MS.gene71139:intron 25.0%
!! TTGATTTTGAACATGGTATA+AGG + chr4.1:56690077-56690096 None:intergenic 25.0%
!!! AGTATTTTGGTCAATTTGTA+TGG + chr4.1:56692019-56692038 None:intergenic 25.0%
!!! CTTTCCAATGTTTGTTTTAT+CGG + chr4.1:56691794-56691813 None:intergenic 25.0%
!!! GATTAAAACTTTTTCTTTGG+AGG - chr4.1:56691116-56691135 MS.gene71139:intron 25.0%
!!! GTAGTATTGATCTATGATTA+TGG + chr4.1:56692161-56692180 None:intergenic 25.0%
!!! GTATTTTGGTCAATTTGTAT+GGG + chr4.1:56692018-56692037 None:intergenic 25.0%
!!! TAGTGAATTGATTTTGAACA+TGG + chr4.1:56690084-56690103 None:intergenic 25.0%
!!! TGATGTTTTTGTACAAAACT+TGG + chr4.1:56692234-56692253 None:intergenic 25.0%
ACATATATGGCAACAAAAAC+AGG - chr4.1:56689565-56689584 MS.gene71139:CDS 30.0%
ACTCTCAATAACATTACTGA+CGG - chr4.1:56689916-56689935 MS.gene71139:intron 30.0%
AGATGGTATATCCTATTCTT+GGG + chr4.1:56689727-56689746 None:intergenic 30.0%
ATGGAGTGAGAATAAAAACT+TGG - chr4.1:56691581-56691600 MS.gene71139:CDS 30.0%
GAACATAGAAATGCAAAATG+TGG - chr4.1:56692129-56692148 MS.gene71139:intron 30.0%
GATTGTATTTCGATAGTTAG+TGG - chr4.1:56690964-56690983 MS.gene71139:CDS 30.0%
GTTCAAAAAGTAAAGCTTAG+TGG - chr4.1:56690022-56690041 MS.gene71139:intron 30.0%
TATGATTCCATATGATCACA+GGG - chr4.1:56692073-56692092 MS.gene71139:intron 30.0%
TCAGTTAATTACGTTACTGA+TGG - chr4.1:56689956-56689975 MS.gene71139:intron 30.0%
TCTTCATTACTCATATGCAA+TGG - chr4.1:56689324-56689343 MS.gene71139:CDS 30.0%
TGCATATGAGTAATGAAGAT+AGG + chr4.1:56689323-56689342 None:intergenic 30.0%
TTATGATTCCATATGATCAC+AGG - chr4.1:56692072-56692091 MS.gene71139:intron 30.0%
! AGAGAAAAGTGTATCTCTAT+TGG + chr4.1:56689460-56689479 None:intergenic 30.0%
! ATCTATAGCTCGAACATTTT+TGG + chr4.1:56690992-56691011 None:intergenic 30.0%
! ATTTTGAACATGGTATAAGG+AGG + chr4.1:56690074-56690093 None:intergenic 30.0%
! GTTTTGTACAAAAACATCAG+AGG - chr4.1:56692235-56692254 MS.gene71139:CDS 30.0%
! TATTATCTTACAGTTGGTCT+TGG + chr4.1:56690531-56690550 None:intergenic 30.0%
!! AAAAAGTTATTGGCTTGAGA+TGG + chr4.1:56689744-56689763 None:intergenic 30.0%
!! AACATTTTGGTCACATAACT+AGG - chr4.1:56689433-56689452 MS.gene71139:CDS 30.0%
AAAACTTGGCAGGTAAAAGT+TGG - chr4.1:56691595-56691614 MS.gene71139:intron 35.0%
AAGTGGATAAAACTTGTCAC+AGG + chr4.1:56691911-56691930 None:intergenic 35.0%
AGATTTGTCAATTGCAGTAC+TGG + chr4.1:56689991-56690010 None:intergenic 35.0%
AGTGAGAATAAAAACTTGGC+AGG - chr4.1:56691585-56691604 MS.gene71139:CDS 35.0%
ATAAACTGTGATATTGGGAC+AGG - chr4.1:56690815-56690834 MS.gene71139:intron 35.0%
ATGCCTTCTTAAATGCCTTA+AGG + chr4.1:56689666-56689685 None:intergenic 35.0%
ATGGTAGTAACGTTCAACAT+TGG + chr4.1:56689379-56689398 None:intergenic 35.0%
CAAAACTTGGCTTAATTGCA+CGG + chr4.1:56692221-56692240 None:intergenic 35.0%
CATGGACCTGAAAAAGTTAT+TGG + chr4.1:56689754-56689773 None:intergenic 35.0%
GAGATGGTATATCCTATTCT+TGG + chr4.1:56689728-56689747 None:intergenic 35.0%
GTCTCGAATTTCAAAGTTGA+TGG - chr4.1:56690212-56690231 MS.gene71139:CDS 35.0%
GTTAGGTAGTTTGAACTAGA+CGG + chr4.1:56691884-56691903 None:intergenic 35.0%
TAGTGTAAGACTCACCATAA+AGG + chr4.1:56689791-56689810 None:intergenic 35.0%
TCAACCAAATACTGCCTTTA+TGG - chr4.1:56689774-56689793 MS.gene71139:intron 35.0%
TCAATCTACCATCATGTACA+TGG + chr4.1:56689398-56689417 None:intergenic 35.0%
TCAATGGCAATGGGAAAAAA+TGG - chr4.1:56690246-56690265 MS.gene71139:intron 35.0%
TCCATTAGTTGTTCCTGTTA+AGG + chr4.1:56691566-56691585 None:intergenic 35.0%
TCGGTTATGCACCTAAAATT+TGG + chr4.1:56691775-56691794 None:intergenic 35.0%
TGAAAAAAGGACCAACAAAG+TGG + chr4.1:56691852-56691871 None:intergenic 35.0%
TGCAAGTATATTTCTCTGCA+AGG + chr4.1:56692310-56692329 None:intergenic 35.0%
TGGAATGAAGCATGTTCTAA+AGG - chr4.1:56689685-56689704 MS.gene71139:intron 35.0%
TGTCAGCATATGAAGAAGAT+AGG - chr4.1:56690165-56690184 MS.gene71139:CDS 35.0%
TTCCAATGTAGTAGTGAACA+GGG - chr4.1:56691537-56691556 MS.gene71139:CDS 35.0%
TTGACAAATCTCCACTTTGT+TGG - chr4.1:56691838-56691857 MS.gene71139:intron 35.0%
TTGCAGAGAAATATACTTGC+AGG - chr4.1:56692309-56692328 MS.gene71139:CDS 35.0%
TTTCCAATGTAGTAGTGAAC+AGG - chr4.1:56691536-56691555 MS.gene71139:CDS 35.0%
! AGGAGAAGAGCAATTTTCTA+TGG + chr4.1:56691978-56691997 None:intergenic 35.0%
! AGTTTTTGTTGCATTACCGA+GGG + chr4.1:56689899-56689918 None:intergenic 35.0%
! CTTTTCTCTTCAAAGTCTCA+AGG - chr4.1:56689471-56689490 MS.gene71139:CDS 35.0%
! GGAATGAAGCATGTTCTAAA+GGG - chr4.1:56689686-56689705 MS.gene71139:intron 35.0%
! TCTCCTTAAGGCATTTAAGA+AGG - chr4.1:56689660-56689679 MS.gene71139:intron 35.0%
! TTAAGGCATTTAAGAAGGCA+TGG - chr4.1:56689665-56689684 MS.gene71139:intron 35.0%
!! AAAACTCACGTGGAGTATTT+TGG + chr4.1:56692032-56692051 None:intergenic 35.0%
!! AATTTCAAAGTTGATGGTGG+TGG - chr4.1:56690218-56690237 MS.gene71139:CDS 35.0%
!! ACGGTTTGGTCAATGAAAAA+AGG + chr4.1:56691865-56691884 None:intergenic 35.0%
!! ATGGTTAGTGTTGATGACTT+TGG - chr4.1:56689495-56689514 MS.gene71139:CDS 35.0%
!! CTCAAGCCAATAACTTTTTC+AGG - chr4.1:56689745-56689764 MS.gene71139:intron 35.0%
!! TCGAATTCATGTTAGCTCTA+TGG - chr4.1:56690119-56690138 MS.gene71139:intron 35.0%
!! TCGAATTTCAAAGTTGATGG+TGG - chr4.1:56690215-56690234 MS.gene71139:CDS 35.0%
!! TTAGCTCTATGGAACAATTG+AGG - chr4.1:56690130-56690149 MS.gene71139:intron 35.0%
AACTTGTCACAGGAGTTGTT+AGG + chr4.1:56691901-56691920 None:intergenic 40.0%
AAGGCAGTATTTGGTTGACA+TGG + chr4.1:56689772-56689791 None:intergenic 40.0%
AATGTCTTAACTGGTGTCTC+CGG + chr4.1:56691417-56691436 None:intergenic 40.0%
AGATGGAAGAGATGATAGAG+AGG - chr4.1:56689527-56689546 MS.gene71139:CDS 40.0%
ATGAAACCTGATTCCATGTC+CGG + chr4.1:56691028-56691047 None:intergenic 40.0%
ATGAAGAAGATAGGCAACAC+TGG - chr4.1:56690174-56690193 MS.gene71139:CDS 40.0%
ATGCAATGGCTCTTACATAG+TGG - chr4.1:56689338-56689357 MS.gene71139:CDS 40.0%
ATGGCAATGGGAAAAAATGG+TGG - chr4.1:56690249-56690268 MS.gene71139:intron 40.0%
CGAGCTATAGATATCACTTG+CGG - chr4.1:56691000-56691019 MS.gene71139:CDS 40.0%
CGTTACTACCATGTACATGA+TGG - chr4.1:56689387-56689406 MS.gene71139:CDS 40.0%
CTCACCATAAAGGCAGTATT+TGG + chr4.1:56689781-56689800 None:intergenic 40.0%
CTCTTCAAAGTCTCAAGGAA+TGG - chr4.1:56689476-56689495 MS.gene71139:CDS 40.0%
GCCTTAACAGGAACAACTAA+TGG - chr4.1:56691562-56691581 MS.gene71139:CDS 40.0%
GGCATCATACGAAATTAGAG+AGG - chr4.1:56690412-56690431 MS.gene71139:intron 40.0%
GTAGTTTGAACTAGACGGTT+TGG + chr4.1:56691879-56691898 None:intergenic 40.0%
TCTATGGAACAATTGAGGCA+TGG - chr4.1:56690135-56690154 MS.gene71139:intron 40.0%
TTCAGCCACATGAATTCCAT+CGG + chr4.1:56690777-56690796 None:intergenic 40.0%
TTTAGACCCTCTGCAATTAC+CGG - chr4.1:56689864-56689883 MS.gene71139:intron 40.0%
! ACATGAATTCCATCGGTGTT+GGG + chr4.1:56690770-56690789 None:intergenic 40.0%
! GAGTTTTTGTTGCATTACCG+AGG + chr4.1:56689900-56689919 None:intergenic 40.0%
! TTCTAAAGGGTCTATCCTTG+TGG - chr4.1:56689699-56689718 MS.gene71139:intron 40.0%
! TTCTGCAGTATTGGAAGCTT+AGG - chr4.1:56691490-56691509 MS.gene71139:CDS 40.0%
! TTGACTTTTTAACCAGTGCC+CGG - chr4.1:56691395-56691414 MS.gene71139:intron 40.0%
! TTTGTCAATTGCAGTACTGG+CGG + chr4.1:56689988-56690007 None:intergenic 40.0%
!! GATGTGATTCTAGAACCAGA+AGG - chr4.1:56692331-56692350 MS.gene71139:CDS 40.0%
!! TCAACATTGGCTTGATGAAG+TGG + chr4.1:56689366-56689385 None:intergenic 40.0%
AATGCTAACCGATGCCTCTA+GGG - chr4.1:56691278-56691297 MS.gene71139:intron 45.0%
AGCCCTGTTCACTACTACAT+TGG + chr4.1:56691542-56691561 None:intergenic 45.0%
ATAGATATCACTTGCGGACC+CGG - chr4.1:56691006-56691025 MS.gene71139:CDS 45.0%
ATATGATCACAGGGTGGTTC+AGG - chr4.1:56692082-56692101 MS.gene71139:intron 45.0%
ATGTCTTAACTGGTGTCTCC+GGG + chr4.1:56691416-56691435 None:intergenic 45.0%
CAACACCGATGGAATTCATG+TGG - chr4.1:56690769-56690788 MS.gene71139:CDS 45.0%
CACATGAATTCCATCGGTGT+TGG + chr4.1:56690771-56690790 None:intergenic 45.0%
GATTCCATATGATCACAGGG+TGG - chr4.1:56692076-56692095 MS.gene71139:intron 45.0%
GGCTGTGATCAACAAGAAGA+GGG + chr4.1:56690495-56690514 None:intergenic 45.0%
GGTCTTGGTTCATCATTGCA+TGG + chr4.1:56690516-56690535 None:intergenic 45.0%
GTGGCACTTTCAATGGCAAT+GGG - chr4.1:56690237-56690256 MS.gene71139:intron 45.0%
TAATGCTAACCGATGCCTCT+AGG - chr4.1:56691277-56691296 MS.gene71139:intron 45.0%
TGAAACCTGATTCCATGTCC+GGG + chr4.1:56691027-56691046 None:intergenic 45.0%
TGAACCACCCTGTGATCATA+TGG + chr4.1:56692083-56692102 None:intergenic 45.0%
TGGCTGTGATCAACAAGAAG+AGG + chr4.1:56690496-56690515 None:intergenic 45.0%
TTCCTTAACTAGTGCCCTAG+AGG + chr4.1:56691295-56691314 None:intergenic 45.0%
TTTCTCTGCAAGGAACGCTT+CGG + chr4.1:56692300-56692319 None:intergenic 45.0%
!! ATTCTAGAACCAGAAGGTGG+TGG - chr4.1:56692337-56692356 MS.gene71139:CDS 45.0%
!! GTGATTCTAGAACCAGAAGG+TGG - chr4.1:56692334-56692353 MS.gene71139:CDS 45.0%
!!! GGGAGTGAGCGTTTTTTGTT+AGG + chr4.1:56691998-56692017 None:intergenic 45.0%
AAACAGAGCCATGCCAAGAG+CGG - chr4.1:56692188-56692207 MS.gene71139:intron 50.0%
GGTGGCACTTTCAATGGCAA+TGG - chr4.1:56690236-56690255 MS.gene71139:intron 50.0%
TCCTATTCTTGGGCACCACA+AGG + chr4.1:56689717-56689736 None:intergenic 50.0%
TCCTTGTGGTGCCCAAGAAT+AGG - chr4.1:56689713-56689732 MS.gene71139:intron 50.0%
! AACTAGTGCCCTAGAGGCAT+CGG + chr4.1:56691289-56691308 None:intergenic 50.0%
!! GACTTTGGAGCAAGAGCAGA+TGG - chr4.1:56689510-56689529 MS.gene71139:CDS 50.0%
!! GATGGTGGTGGCACTTTCAA+TGG - chr4.1:56690230-56690249 MS.gene71139:CDS 50.0%
!! TGCCTCTAGGGCACTAGTTA+AGG - chr4.1:56691290-56691309 MS.gene71139:intron 50.0%
!! TTTGATTGTGAGAGCGCCAG+AGG - chr4.1:56690739-56690758 MS.gene71139:CDS 50.0%
AGACCCTCTGCAATTACCGG+CGG - chr4.1:56689867-56689886 MS.gene71139:intron 55.0%
AGACCGCCGGTAATTGCAGA+GGG + chr4.1:56689873-56689892 None:intergenic 55.0%
GAGGATAGTCCCAACACCGA+TGG - chr4.1:56690758-56690777 MS.gene71139:CDS 55.0%
GTAGTTCCTCCACCACCTTC+TGG + chr4.1:56692349-56692368 None:intergenic 55.0%
GTGAACAGGGCTGCCTTAAC+AGG - chr4.1:56691550-56691569 MS.gene71139:CDS 55.0%
TAGACCGCCGGTAATTGCAG+AGG + chr4.1:56689874-56689893 None:intergenic 55.0%
TTACCGAGGGTTTAGACCGC+CGG + chr4.1:56689886-56689905 None:intergenic 55.0%
TTACCGGCGGTCTAAACCCT+CGG - chr4.1:56689880-56689899 MS.gene71139:intron 55.0%
! CTAGAACCAGAAGGTGGTGG+AGG - chr4.1:56692340-56692359 MS.gene71139:CDS 55.0%
! GCTCTTGGCATGGCTCTGTT+TGG + chr4.1:56692189-56692208 None:intergenic 55.0%
! GCTTTGTTCCGCTCTTGGCA+TGG + chr4.1:56692199-56692218 None:intergenic 55.0%
!! TCGGTGTTGGGACTATCCTC+TGG + chr4.1:56690758-56690777 None:intergenic 55.0%
ATCACTTGCGGACCCGGACA+TGG - chr4.1:56691012-56691031 MS.gene71139:CDS 60.0%
TAACTGGTGTCTCCGGGCAC+TGG + chr4.1:56691410-56691429 None:intergenic 60.0%
!! GCACGGCTTTGTTCCGCTCT+TGG + chr4.1:56692204-56692223 None:intergenic 60.0%
GCGGACCCGGACATGGAATC+AGG - chr4.1:56691019-56691038 MS.gene71139:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 56689312 56692435 56689312 ID=MS.gene71139
chr4.1 mRNA 56689312 56692435 56689312 ID=MS.gene71139.t1;Parent=MS.gene71139
chr4.1 exon 56692199 56692435 56692199 ID=MS.gene71139.t1.exon1;Parent=MS.gene71139.t1
chr4.1 CDS 56692199 56692435 56692199 ID=cds.MS.gene71139.t1;Parent=MS.gene71139.t1
chr4.1 exon 56691952 56692077 56691952 ID=MS.gene71139.t1.exon2;Parent=MS.gene71139.t1
chr4.1 CDS 56691952 56692077 56691952 ID=cds.MS.gene71139.t1;Parent=MS.gene71139.t1
chr4.1 exon 56691443 56691613 56691443 ID=MS.gene71139.t1.exon3;Parent=MS.gene71139.t1
chr4.1 CDS 56691443 56691613 56691443 ID=cds.MS.gene71139.t1;Parent=MS.gene71139.t1
chr4.1 exon 56691208 56691234 56691208 ID=MS.gene71139.t1.exon4;Parent=MS.gene71139.t1
chr4.1 CDS 56691208 56691234 56691208 ID=cds.MS.gene71139.t1;Parent=MS.gene71139.t1
chr4.1 exon 56690911 56691118 56690911 ID=MS.gene71139.t1.exon5;Parent=MS.gene71139.t1
chr4.1 CDS 56690911 56691118 56690911 ID=cds.MS.gene71139.t1;Parent=MS.gene71139.t1
chr4.1 exon 56690707 56690788 56690707 ID=MS.gene71139.t1.exon6;Parent=MS.gene71139.t1
chr4.1 CDS 56690707 56690788 56690707 ID=cds.MS.gene71139.t1;Parent=MS.gene71139.t1
chr4.1 exon 56690141 56690249 56690141 ID=MS.gene71139.t1.exon7;Parent=MS.gene71139.t1
chr4.1 CDS 56690141 56690249 56690141 ID=cds.MS.gene71139.t1;Parent=MS.gene71139.t1
chr4.1 exon 56689312 56689653 56689312 ID=MS.gene71139.t1.exon8;Parent=MS.gene71139.t1
chr4.1 CDS 56689312 56689653 56689312 ID=cds.MS.gene71139.t1;Parent=MS.gene71139.t1
Gene Sequence

>MS.gene71139

ATGATATTCCTATCTTCATTACTCATATGCAATGGCTCTTACATAGTGGAACCACTTCATCAAGCCAATGTTGAACGTTACTACCATGTACATGATGGTAGATTGATCAAAACAAAACATCAACATTTTGGTCACATAACTAGGACCAATAGAGATACACTTTTCTCTTCAAAGTCTCAAGGAATGGTTAGTGTTGATGACTTTGGAGCAAGAGCAGATGGAAGAGATGATAGAGAGGTAAAAAATAACAATAACATATATGGCAACAAAAACAGGAAAATTTATTATGTTAATTTATTCTTAACAACAATATTCTCAAACTTTCTCACTTTTTCATTTCATTTAATTTCTCCTTAAGGCATTTAAGAAGGCATGGAATGAAGCATGTTCTAAAGGGTCTATCCTTGTGGTGCCCAAGAATAGGATATACCATCTCAAGCCAATAACTTTTTCAGGTCCATGTCAACCAAATACTGCCTTTATGGTGAGTCTTACACTACAAGAATTCTCTTTATTAGTGACCAATTTAAAATTGCATTACTGAATTATATATTTAGACCCTCTGCAATTACCGGCGGTCTAAACCCTCGGTAATGCAACAAAAACTCTCAATAACATTACTGACGGAATAAATCTTTAATAATTCAGTTAATTACGTTACTGATGGATAAATCCGCCAGTACTGCAATTGACAAATCTGCCTTAGATTAGTTCAAAAAGTAAAGCTTAGTGGGACACTCAATTAGATAGTTTTTGTTTCCTCCTTATACCATGTTCAAAATCAATTCACTATAATTGTTTTATTTTTCGAATTCATGTTAGCTCTATGGAACAATTGAGGCATGGTCTCAAATGTCAGCATATGAAGAAGATAGGCAACACTGGATTGTGTTTGATAGTGTCTCGAATTTCAAAGTTGATGGTGGTGGCACTTTCAATGGCAATGGGAAAAAATGGTGGCAAAACTCATGCAAATATTCCAACAATAACCTTGTAAGTTTTTGTACCTTTTTTTTTCTTCCAATTTTCATTTGTTATATTTTCTTTTAAACTAAATTATGAAAAATACTTGAGAGAGGAAATGAATGATTTCAAATTTTGGCATCATACGAAATTAGAGAGGAGTTATATTTGTCATCAACTTTTTTCTATTAGATGAATTTTGACCTTAATTTATTTTCCCTCTTCTTGTTGATCACAGCCATGCAATGATGAACCAAGACCAACTGTAAGATAATATTCTTCAAACCATAAATTTTTACAAGTATATTTATTTTCTAATCGATTCATACTTTAATGGAAAATAAATTTATGTAGGCGGTGACATTCTATGATTGCAAAAATTTGAAAGTAAAGAATCTAAGGTTCAAAGATGCACAACAAATGCATGTAGCATTTGAGAGATGCTTCAATGTGTTTGTTTCGAATTTGATTGTGAGAGCGCCAGAGGATAGTCCCAACACCGATGGAATTCATGTGGCTGAAACACAAAATATTGACATAATAAACTGTGATATTGGGACAGGTATGTATAAAAAAAGAGAAAATATTTCAAATAAAAAACATTCAAAAAATAAATATTTGGTTGCTAAATAAAAAACATTCAATTGGATTTTAATTTTTATTTTACTTTCTTTTTTGTTGAAGGTGATGATTGTATTTCGATAGTTAGTGGATCCAAAAATGTTCGAGCTATAGATATCACTTGCGGACCCGGACATGGAATCAGGTTTCATTGTTATATTATATTAATCCTCATTTAATTCATAGATTGAGTTTTGTTTTGATCAATTTTTAAGAGATTGATTAAAACTTTTTCTTTGGAGGGAAGATTGATTAAAACTTTAAAGTTGAGATTGGGAGAAGATTCTAGTCACTATAGATCTCAAAAATTAAGATGCAATAGATAATTTCAACATATATTTTTTTTTTGAGGGAAATAATTTCAACATATATGAAAAAGGGTAATGCTAACCGATGCCTCTAGGGCACTAGTTAAGGAAGCCAAAAGAGAAAATTTAAAATAAAAAATGATATATTTGAAGTATTAATTACATAACTTTAAATGCAAATATTATTATATTTGACTTTTTAACCAGTGCCCGGAGACACCAGTTAAGACATTTTTTTATATTTGCTTTTCTGGACTACTTATTGAGAAGTACTCATTAATATATATTCTGCAGTATTGGAAGCTTAGGAGCTGATAACTCAGAAGCTGAAGTTTCCAATGTAGTAGTGAACAGGGCTGCCTTAACAGGAACAACTAATGGAGTGAGAATAAAAACTTGGCAGGTAAAAGTTGGTCTTAAGATTTTGAAAATTAGTCTGTTAGGCACTCTACAACTAACTTTGTGAGATGAAAGAATGAACTAATTAATAGAAGTCAAAAAATAAACTTTCATAAAGTAGAATTTCATCTAATCATGTAAATCTTGTTGACTTTCTATAAGCTATTTGCCAAATTTTAGGTGCATAACCGATAAAACAAACATTGGAAAGTTGATGAAATTAGTGCAAACAACTTTGACAAATCTCCACTTTGTTGGTCCTTTTTTCATTGACCAAACCGTCTAGTTCAAACTACCTAACAACTCCTGTGACAAGTTTTATCCACTTATTAATTTCTTCTAAATGTCTCAATGACGACCTTTAATTAATAACCATAGAAAATTGCTCTTCTCCTAACAAAAAACGCTCACTCCCATACAAATTGACCAAAATACTCCACGTGAGTTTTATTACAATATATATCTTATATTATGATTCCATATGATCACAGGGTGGTTCAGGATATGCAAGAAACATCAAATTTATGAACATAGAAATGCAAAATGTGGCAAATCCCATAATCATAGATCAATACTACTGCGACCAAACAGAGCCATGCCAAGAGCGGAACAAAGCCGTGCAATTAAGCCAAGTTTTGTACAAAAACATCAGAGGAACGAGTGCTTCAGAAGTAGCGATCAAATTTAATTGCAGCCGAAGCGTTCCTTGCAGAGAAATATACTTGCAGGATGTGATTCTAGAACCAGAAGGTGGTGGAGGAACTACTGCAACATGTGAGAATGTTAGATATGTAAATAGAGGAAAGTTTTATCCTCAATGCTCTCCACAATAG
Protein sequence

>MS.gene71139.t1

MIFLSSLLICNGSYIVEPLHQANVERYYHVHDGRLIKTKHQHFGHITRTNRDTLFSSKSQGMVSVDDFGARADGRDDREAFKKAWNEACSKGSILVVPKNRIYHLKPITFSGPCQPNTAFMLYGTIEAWSQMSAYEEDRQHWIVFDSVSNFKVDGGGTFNGNGKKWWQNSCKYSNNNLPCNDEPRPTAVTFYDCKNLKVKNLRFKDAQQMHVAFERCFNVFVSNLIVRAPEDSPNTDGIHVAETQNIDIINCDIGTGDDCISIVSGSKNVRAIDITCGPGHGISIGSLGADNSEAEVSNVVVNRAALTGTTNGVRIKTWQGGSGYARNIKFMNIEMQNVANPIIIDQYYCDQTEPCQERNKAVQLSQVLYKNIRGTSASEVAIKFNCSRSVPCREIYLQDVILEPEGGGGTTATCENVRYVNRGKFYPQCSPQ