Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71264.t1 | XP_013443446.1 | 97.1 | 68 | 2 | 0 | 1 | 68 | 1 | 68 | 3.00E-32 | 147.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71264.t1 | Q9LRH8 | 92.6 | 68 | 5 | 0 | 1 | 68 | 1 | 68 | 1.8e-33 | 142.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71264.t1 | A0A072TJY2 | 97.1 | 68 | 2 | 0 | 1 | 68 | 1 | 68 | 2.2e-32 | 147.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene71264 | 0.801673 | 8.06E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene71264 | 0.83258 | 8.72E-56 | -1.69E-46 |
| MS.gene051719 | MS.gene71264 | 0.802421 | 5.66E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene71264 | 0.811152 | 8.08E-51 | -1.69E-46 |
| MS.gene052665 | MS.gene71264 | 0.818917 | 1.53E-52 | -1.69E-46 |
| MS.gene053581 | MS.gene71264 | 0.806667 | 7.36E-50 | -1.69E-46 |
| MS.gene05364 | MS.gene71264 | 0.80274 | 4.86E-49 | -1.69E-46 |
| MS.gene055465 | MS.gene71264 | 0.816671 | 4.90E-52 | -1.69E-46 |
| MS.gene055466 | MS.gene71264 | 0.806083 | 9.77E-50 | -1.69E-46 |
| MS.gene055468 | MS.gene71264 | 0.808445 | 3.09E-50 | -1.69E-46 |
| MS.gene055890 | MS.gene71264 | 0.802669 | 5.03E-49 | -1.69E-46 |
| MS.gene055891 | MS.gene71264 | 0.812326 | 4.49E-51 | -1.69E-46 |
| MS.gene056005 | MS.gene71264 | 0.812152 | 4.89E-51 | -1.69E-46 |
| MS.gene058852 | MS.gene71264 | 0.802179 | 6.34E-49 | -1.69E-46 |
| MS.gene060519 | MS.gene71264 | 0.804572 | 2.03E-49 | -1.69E-46 |
| MS.gene060587 | MS.gene71264 | 0.803498 | 3.39E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71264.t1 | MTR_0013s0230 | 97.059 | 68 | 2 | 0 | 1 | 68 | 1 | 68 | 3.14e-41 | 142 |
| MS.gene71264.t1 | MTR_3g436170 | 78.571 | 70 | 15 | 0 | 1 | 70 | 1 | 70 | 4.20e-40 | 126 |
| MS.gene71264.t1 | MTR_8g017040 | 77.612 | 67 | 15 | 0 | 1 | 67 | 1 | 67 | 3.84e-38 | 123 |
| MS.gene71264.t1 | MTR_4g005190 | 89.706 | 68 | 7 | 0 | 1 | 68 | 1 | 68 | 6.44e-38 | 132 |
| MS.gene71264.t1 | MTR_4g005190 | 89.706 | 68 | 7 | 0 | 1 | 68 | 1 | 68 | 7.17e-38 | 132 |
| MS.gene71264.t1 | MTR_4g005270 | 89.706 | 68 | 7 | 0 | 1 | 68 | 1 | 68 | 1.56e-37 | 131 |
| MS.gene71264.t1 | MTR_8g016975 | 77.941 | 68 | 15 | 0 | 1 | 68 | 1 | 68 | 5.95e-36 | 127 |
| MS.gene71264.t1 | MTR_8g016960 | 75.000 | 68 | 17 | 0 | 1 | 68 | 1 | 68 | 1.50e-33 | 120 |
| MS.gene71264.t1 | MTR_6g093180 | 51.471 | 68 | 31 | 1 | 1 | 68 | 1 | 66 | 1.11e-17 | 75.5 |
| MS.gene71264.t1 | MTR_5g008810 | 52.239 | 67 | 31 | 1 | 1 | 67 | 1 | 66 | 1.38e-17 | 75.1 |
| MS.gene71264.t1 | MTR_6g035010 | 51.471 | 68 | 31 | 1 | 1 | 68 | 1 | 66 | 2.38e-17 | 74.3 |
| MS.gene71264.t1 | MTR_6g034985 | 50.000 | 68 | 32 | 1 | 1 | 68 | 1 | 66 | 3.19e-17 | 73.9 |
| MS.gene71264.t1 | MTR_7g062020 | 49.254 | 67 | 34 | 0 | 1 | 67 | 1 | 67 | 3.22e-16 | 71.2 |
| MS.gene71264.t1 | MTR_6g088140 | 50.000 | 70 | 33 | 1 | 1 | 68 | 1 | 70 | 4.94e-16 | 70.9 |
| MS.gene71264.t1 | MTR_6g088140 | 50.000 | 70 | 33 | 1 | 1 | 68 | 1 | 70 | 5.64e-16 | 70.5 |
| MS.gene71264.t1 | MTR_6g093100 | 50.725 | 69 | 32 | 1 | 1 | 67 | 1 | 69 | 1.79e-15 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71264.t1 | AT1G66960 | 67.647 | 68 | 21 | 1 | 1 | 67 | 1 | 68 | 7.96e-29 | 107 |
| MS.gene71264.t1 | AT1G66960 | 67.647 | 68 | 21 | 1 | 1 | 67 | 1 | 68 | 7.96e-29 | 107 |
| MS.gene71264.t1 | AT1G66960 | 67.647 | 68 | 21 | 1 | 1 | 67 | 1 | 68 | 7.96e-29 | 107 |
| MS.gene71264.t1 | AT1G66960 | 67.647 | 68 | 21 | 1 | 1 | 67 | 10 | 77 | 7.97e-29 | 107 |
| MS.gene71264.t1 | AT1G78960 | 66.176 | 68 | 22 | 1 | 1 | 67 | 1 | 68 | 2.62e-27 | 102 |
| MS.gene71264.t1 | AT1G78960 | 66.176 | 68 | 22 | 1 | 1 | 67 | 1 | 68 | 2.62e-27 | 102 |
| MS.gene71264.t1 | AT1G78960 | 66.176 | 68 | 22 | 1 | 1 | 67 | 1 | 68 | 2.62e-27 | 102 |
| MS.gene71264.t1 | AT1G78960 | 66.176 | 68 | 22 | 1 | 1 | 67 | 1 | 68 | 3.23e-27 | 102 |
| MS.gene71264.t1 | AT1G78950 | 67.647 | 68 | 21 | 1 | 1 | 67 | 10 | 77 | 1.58e-26 | 100 |
| MS.gene71264.t1 | AT1G78950 | 67.647 | 68 | 21 | 1 | 1 | 67 | 1 | 68 | 1.76e-26 | 100 |
| MS.gene71264.t1 | AT1G78970 | 64.706 | 68 | 23 | 1 | 1 | 67 | 1 | 68 | 4.54e-25 | 96.3 |
| MS.gene71264.t1 | AT1G78970 | 64.706 | 68 | 23 | 1 | 1 | 67 | 1 | 68 | 4.54e-25 | 96.3 |
| MS.gene71264.t1 | AT1G78970 | 64.706 | 68 | 23 | 1 | 1 | 67 | 1 | 68 | 4.54e-25 | 96.3 |
| MS.gene71264.t1 | AT1G78955 | 64.706 | 68 | 23 | 1 | 1 | 67 | 1 | 68 | 6.14e-25 | 96.3 |
| MS.gene71264.t1 | AT5G36150 | 55.882 | 68 | 29 | 1 | 1 | 67 | 1 | 68 | 2.68e-21 | 85.9 |
| MS.gene71264.t1 | AT5G36150 | 55.882 | 68 | 29 | 1 | 1 | 67 | 1 | 68 | 2.68e-21 | 85.9 |
| MS.gene71264.t1 | AT4G15340 | 58.824 | 68 | 27 | 1 | 1 | 67 | 1 | 68 | 3.45e-21 | 85.5 |
| MS.gene71264.t1 | AT4G15340 | 58.824 | 68 | 27 | 1 | 1 | 67 | 1 | 68 | 4.14e-21 | 85.1 |
| MS.gene71264.t1 | AT4G15370 | 58.824 | 68 | 27 | 1 | 1 | 67 | 1 | 68 | 7.97e-21 | 84.3 |
| MS.gene71264.t1 | AT4G15370 | 58.824 | 68 | 27 | 1 | 1 | 67 | 1 | 68 | 8.12e-21 | 84.3 |
| MS.gene71264.t1 | AT1G78500 | 57.353 | 68 | 28 | 1 | 1 | 67 | 1 | 68 | 1.01e-19 | 81.3 |
| MS.gene71264.t1 | AT1G78500 | 57.353 | 68 | 28 | 1 | 1 | 67 | 1 | 68 | 1.01e-19 | 81.3 |
| MS.gene71264.t1 | AT2G07050 | 58.209 | 67 | 27 | 1 | 1 | 67 | 1 | 66 | 1.05e-18 | 78.2 |
| MS.gene71264.t1 | AT5G42600 | 55.882 | 68 | 29 | 1 | 1 | 67 | 1 | 68 | 1.63e-18 | 77.8 |
| MS.gene71264.t1 | AT5G48010 | 47.059 | 68 | 35 | 1 | 1 | 67 | 1 | 68 | 6.54e-16 | 70.5 |
| MS.gene71264.t1 | AT5G48010 | 47.059 | 68 | 35 | 1 | 1 | 67 | 1 | 68 | 7.14e-16 | 70.5 |
| MS.gene71264.t1 | AT3G29255 | 50.000 | 64 | 31 | 1 | 2 | 64 | 6 | 69 | 9.18e-16 | 70.1 |
| MS.gene71264.t1 | AT3G45130 | 49.254 | 67 | 30 | 1 | 1 | 67 | 1 | 63 | 3.47e-14 | 65.5 |
| MS.gene71264.t1 | AT3G45130 | 49.254 | 67 | 30 | 1 | 1 | 67 | 1 | 63 | 3.47e-14 | 65.5 |
| MS.gene71264.t1 | AT3G45130 | 49.254 | 67 | 30 | 1 | 1 | 67 | 1 | 63 | 3.47e-14 | 65.5 |
| MS.gene71264.t1 | AT3G45130 | 49.254 | 67 | 30 | 1 | 1 | 67 | 1 | 63 | 3.72e-14 | 65.5 |
| MS.gene71264.t1 | AT3G45130 | 49.254 | 67 | 30 | 1 | 1 | 67 | 1 | 63 | 3.72e-14 | 65.5 |
Find 22 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCTTCACTACCTGCTTC+TGG | 0.268680 | 4.2:+64307695 | None:intergenic |
| TTATTTGTACTGAATAAGTA+TGG | 0.274999 | 4.2:+64307749 | None:intergenic |
| TTTCTATAACAAACGCTTCA+AGG | 0.383261 | 4.2:-64307642 | MS.gene71264:CDS |
| AGCCCTGCGGTGATCTCCTC+TGG | 0.402392 | 4.2:-64307616 | MS.gene71264:CDS |
| TTCAGTACAAATAACTTTGT+GGG | 0.434097 | 4.2:-64307740 | MS.gene71264:CDS |
| ATTCAGTACAAATAACTTTG+TGG | 0.439961 | 4.2:-64307741 | MS.gene71264:CDS |
| TCTTACGAGCTTCTTCAACT+TGG | 0.447078 | 4.2:+64307666 | None:intergenic |
| CTTACGAGCTTCTTCAACTT+GGG | 0.467021 | 4.2:+64307667 | None:intergenic |
| ACGCCAGAGGAGATCACCGC+AGG | 0.499083 | 4.2:+64307613 | None:intergenic |
| GGTTGAAGATAGCAGAAGGT+GGG | 0.522657 | 4.2:-64307778 | MS.gene71264:CDS |
| TATTTGTACTGAATAAGTAT+GGG | 0.564113 | 4.2:+64307750 | None:intergenic |
| AGGTTGAAGATAGCAGAAGG+TGG | 0.567732 | 4.2:-64307779 | MS.gene71264:CDS |
| TGGAGGTTGAAGATAGCAGA+AGG | 0.575453 | 4.2:-64307782 | MS.gene71264:CDS |
| TGGGAGTATGATCCAGAAGC+AGG | 0.576042 | 4.2:-64307707 | MS.gene71264:CDS |
| CGCCAGAGGAGATCACCGCA+GGG | 0.613959 | 4.2:+64307614 | None:intergenic |
| CAAAAGAACGAAAGATGTGG+AGG | 0.638609 | 4.2:-64307799 | None:intergenic |
| CGCTTCAAGGTCAAGCCCTG+CGG | 0.647899 | 4.2:-64307629 | MS.gene71264:CDS |
| AAGCAGGTAGTGAAGAAGAG+AGG | 0.648505 | 4.2:-64307691 | MS.gene71264:CDS |
| CTTTGTGGGAAGACAAACAT+GGG | 0.648543 | 4.2:-64307726 | MS.gene71264:CDS |
| ACTTTGTGGGAAGACAAACA+TGG | 0.666531 | 4.2:-64307727 | MS.gene71264:CDS |
| ACATAGTATAGCCATTAACC+AGG | 0.686248 | 4.2:+64307581 | None:intergenic |
| CAGGTACCTGAAAACGCCAG+AGG | 0.733364 | 4.2:+64307600 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTTGTACTGAATAAGTAT+GGG | + | chr4.2:64307633-64307652 | None:intergenic | 20.0% |
| !! | TTATTTGTACTGAATAAGTA+TGG | + | chr4.2:64307634-64307653 | None:intergenic | 20.0% |
| ! | ATTCAGTACAAATAACTTTG+TGG | - | chr4.2:64307639-64307658 | MS.gene71264:CDS | 25.0% |
| ! | TTCAGTACAAATAACTTTGT+GGG | - | chr4.2:64307640-64307659 | MS.gene71264:CDS | 25.0% |
| TTTCTATAACAAACGCTTCA+AGG | - | chr4.2:64307738-64307757 | MS.gene71264:CDS | 30.0% | |
| ACTTTGTGGGAAGACAAACA+TGG | - | chr4.2:64307653-64307672 | MS.gene71264:CDS | 40.0% | |
| CTTACGAGCTTCTTCAACTT+GGG | + | chr4.2:64307716-64307735 | None:intergenic | 40.0% | |
| CTTTGTGGGAAGACAAACAT+GGG | - | chr4.2:64307654-64307673 | MS.gene71264:CDS | 40.0% | |
| TCTTACGAGCTTCTTCAACT+TGG | + | chr4.2:64307717-64307736 | None:intergenic | 40.0% | |
| AAGCAGGTAGTGAAGAAGAG+AGG | - | chr4.2:64307689-64307708 | MS.gene71264:CDS | 45.0% | |
| AGGTTGAAGATAGCAGAAGG+TGG | - | chr4.2:64307601-64307620 | MS.gene71264:CDS | 45.0% | |
| GGTTGAAGATAGCAGAAGGT+GGG | - | chr4.2:64307602-64307621 | MS.gene71264:CDS | 45.0% | |
| TCTTCTTCACTACCTGCTTC+TGG | + | chr4.2:64307688-64307707 | None:intergenic | 45.0% | |
| TGGAGGTTGAAGATAGCAGA+AGG | - | chr4.2:64307598-64307617 | MS.gene71264:CDS | 45.0% | |
| ! | TGATCTCCTCTGGCGTTTTC+AGG | - | chr4.2:64307774-64307793 | MS.gene71264:CDS | 50.0% |
| ! | TGGGAGTATGATCCAGAAGC+AGG | - | chr4.2:64307673-64307692 | MS.gene71264:CDS | 50.0% |
| CAGGTACCTGAAAACGCCAG+AGG | + | chr4.2:64307783-64307802 | None:intergenic | 55.0% | |
| !!! | CTCTGGCGTTTTCAGGTACC+TGG | - | chr4.2:64307781-64307800 | MS.gene71264:CDS | 55.0% |
| CGCTTCAAGGTCAAGCCCTG+CGG | - | chr4.2:64307751-64307770 | MS.gene71264:CDS | 60.0% | |
| ACGCCAGAGGAGATCACCGC+AGG | + | chr4.2:64307770-64307789 | None:intergenic | 65.0% | |
| AGCCCTGCGGTGATCTCCTC+TGG | - | chr4.2:64307764-64307783 | MS.gene71264:CDS | 65.0% | |
| CGCCAGAGGAGATCACCGCA+GGG | + | chr4.2:64307769-64307788 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 64307595 | 64307807 | 64307595 | ID=MS.gene71264 |
| chr4.2 | mRNA | 64307595 | 64307807 | 64307595 | ID=MS.gene71264.t1;Parent=MS.gene71264 |
| chr4.2 | exon | 64307595 | 64307807 | 64307595 | ID=MS.gene71264.t1.exon1;Parent=MS.gene71264.t1 |
| chr4.2 | CDS | 64307595 | 64307807 | 64307595 | ID=cds.MS.gene71264.t1;Parent=MS.gene71264.t1 |
| Gene Sequence |
| Protein sequence |
