AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72238

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72238.t1 MTR_6g088320 97.683 259 6 0 1 259 30 288 0.0 540
MS.gene72238.t1 MTR_8g102910 77.220 259 59 0 1 259 26 284 9.89e-160 444
MS.gene72238.t1 MTR_4g128410 55.118 254 108 2 3 254 31 280 7.30e-101 295
MS.gene72238.t1 MTR_4g126920 55.118 254 109 3 3 254 31 281 1.25e-100 295
MS.gene72238.t1 MTR_2g038860 53.053 262 114 3 3 258 32 290 7.59e-100 293
MS.gene72238.t1 MTR_2g038705 53.053 262 114 3 3 258 32 290 7.59e-100 293
MS.gene72238.t1 MTR_7g093530 54.724 254 109 2 3 254 30 279 4.06e-98 288
MS.gene72238.t1 MTR_4g128400 55.906 254 106 2 3 254 34 283 4.79e-98 288
MS.gene72238.t1 MTR_4g128570 54.331 254 96 3 3 254 37 272 1.49e-95 281
MS.gene72238.t1 MTR_4g128580 55.642 257 106 5 3 254 32 285 3.94e-95 281
MS.gene72238.t1 MTR_3g084620 51.351 259 114 2 3 259 34 282 3.44e-92 273
MS.gene72238.t1 MTR_3g084630 51.351 259 114 2 3 259 34 282 4.19e-92 273
MS.gene72238.t1 MTR_7g084760 49.035 259 126 4 2 258 37 291 4.04e-91 271
MS.gene72238.t1 MTR_7g084770 49.412 255 123 4 2 254 37 287 6.82e-91 270
MS.gene72238.t1 MTR_4g128590 52.713 258 97 4 3 254 37 275 2.70e-90 268
MS.gene72238.t1 MTR_8g081040 51.181 254 113 3 3 254 37 281 9.56e-90 267
MS.gene72238.t1 MTR_7g084750 49.012 253 123 4 4 254 39 287 1.14e-89 267
MS.gene72238.t1 MTR_2g089140 46.693 257 131 2 5 256 33 288 5.62e-88 263
MS.gene72238.t1 MTR_6g033085 49.407 253 122 4 4 254 39 287 6.24e-87 260
MS.gene72238.t1 MTR_6g033055 48.617 253 124 4 4 254 39 287 6.96e-87 260
MS.gene72238.t1 MTR_4g097700 49.225 258 123 5 1 255 73 325 1.50e-86 261
MS.gene72238.t1 MTR_4g097640 48.846 260 125 5 1 257 36 290 1.92e-86 259
MS.gene72238.t1 MTR_4g097670 48.846 260 125 5 1 257 36 290 1.92e-86 259
MS.gene72238.t1 MTR_4g045853 46.484 256 131 2 4 254 34 288 3.08e-85 256
MS.gene72238.t1 MTR_1g105900 45.703 256 135 2 1 254 38 291 1.29e-84 254
MS.gene72238.t1 MTR_3g089540 49.180 244 117 1 13 256 48 284 6.09e-82 248
MS.gene72238.t1 MTR_8g102220 47.287 258 112 4 3 258 35 270 1.54e-80 243
MS.gene72238.t1 MTR_2g089140 48.673 226 110 2 36 256 1 225 1.12e-79 239
MS.gene72238.t1 MTR_8g013055 49.219 256 121 6 3 255 37 286 2.96e-79 241
MS.gene72238.t1 MTR_1g105495 44.358 257 135 4 3 254 41 294 4.77e-76 233
MS.gene72238.t1 MTR_5g091730 46.473 241 120 2 14 254 47 278 1.04e-75 232
MS.gene72238.t1 MTR_4g057450 40.541 259 133 7 5 254 58 304 1.30e-60 194
MS.gene72238.t1 MTR_7g056090 39.608 255 140 7 3 254 38 281 1.90e-60 192
MS.gene72238.t1 MTR_2g095800 39.768 259 136 7 5 254 62 309 4.52e-60 192
MS.gene72238.t1 MTR_2g049130 41.423 239 130 7 5 240 37 268 1.24e-57 185
MS.gene72238.t1 MTR_7g102260 39.535 258 137 7 5 254 45 291 2.82e-57 185
MS.gene72238.t1 MTR_1g063020 38.760 258 139 6 5 254 48 294 3.81e-57 184
MS.gene72238.t1 MTR_2g049030 38.735 253 138 8 5 254 38 276 2.81e-55 179
MS.gene72238.t1 MTR_8g064180 34.677 248 153 5 14 254 65 310 1.77e-48 162
MS.gene72238.t1 MTR_1g033420 35.019 257 154 7 5 254 41 291 3.47e-48 162
MS.gene72238.t1 MTR_5g029100 36.653 251 145 8 13 254 60 305 9.05e-45 154
MS.gene72238.t1 MTR_8g090100 33.460 263 152 11 5 254 29 281 5.22e-43 149
MS.gene72238.t1 MTR_5g029100 37.611 226 127 8 38 254 54 274 5.46e-41 143
MS.gene72238.t1 MTR_8g479340 42.857 98 53 2 157 254 41 135 4.49e-20 84.0
MS.gene72238.t1 MTR_8g032640 34.706 170 103 4 17 184 33 196 8.64e-18 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72238.t1 AT4G28850 65.504 258 88 1 3 259 35 292 5.81e-133 377
MS.gene72238.t1 AT5G57530 57.087 254 103 3 3 254 33 282 5.29e-100 293
MS.gene72238.t1 AT5G57540 55.118 254 108 3 3 254 32 281 2.66e-97 286
MS.gene72238.t1 AT5G57550 55.118 254 105 4 3 254 36 282 1.14e-96 285
MS.gene72238.t1 AT5G57560 53.668 259 113 4 3 257 29 284 2.84e-96 284
MS.gene72238.t1 AT4G25810 53.876 258 113 3 3 257 32 286 5.84e-95 281
MS.gene72238.t1 AT3G23730 50.775 258 125 1 3 258 33 290 9.04e-95 280
MS.gene72238.t1 AT2G18800 53.383 266 107 6 3 254 34 296 1.57e-94 280
MS.gene72238.t1 AT4G14130 50.000 264 115 3 3 258 34 288 4.25e-93 276
MS.gene72238.t1 AT4G25820 52.490 261 104 4 3 254 36 285 5.40e-92 273
MS.gene72238.t1 AT4G30270 50.787 254 105 4 3 254 30 265 6.21e-90 267
MS.gene72238.t1 AT5G48070 50.588 255 115 5 3 254 35 281 4.55e-88 263
MS.gene72238.t1 AT4G03210 47.809 251 129 2 4 254 36 284 1.05e-87 262
MS.gene72238.t1 AT5G13870 47.843 255 127 4 2 254 37 287 7.50e-86 258
MS.gene72238.t1 AT5G13870 47.843 255 127 4 2 254 37 287 7.50e-86 258
MS.gene72238.t1 AT5G13870 47.843 255 127 4 2 254 37 287 7.50e-86 258
MS.gene72238.t1 AT4G37800 49.020 255 122 5 3 254 40 289 1.13e-84 254
MS.gene72238.t1 AT4G30290 50.588 255 115 4 3 254 30 276 1.01e-83 252
MS.gene72238.t1 AT2G06850 48.963 241 119 3 14 254 54 290 1.52e-83 252
MS.gene72238.t1 AT2G14620 46.484 256 133 2 1 254 41 294 2.61e-83 251
MS.gene72238.t1 AT5G65730 48.031 254 122 6 5 254 43 290 1.45e-82 249
MS.gene72238.t1 AT4G30280 50.655 229 105 3 26 254 61 281 3.72e-81 245
MS.gene72238.t1 AT4G03210 49.333 225 112 2 30 254 34 256 5.06e-81 244
MS.gene72238.t1 AT2G14620 48.472 229 116 1 26 254 18 244 1.63e-79 240
MS.gene72238.t1 AT1G65310 50.218 229 106 3 26 254 61 281 1.83e-79 241
MS.gene72238.t1 AT1G65310 50.870 230 103 4 26 254 83 303 2.71e-79 241
MS.gene72238.t1 AT2G14620 50.000 220 108 1 35 254 16 233 1.19e-78 238
MS.gene72238.t1 AT2G06850 48.261 230 115 3 25 254 5 230 2.49e-78 236
MS.gene72238.t1 AT4G13090 47.059 255 128 5 3 254 38 288 1.16e-77 237
MS.gene72238.t1 AT4G13080 46.667 255 125 6 3 254 45 291 6.40e-75 230
MS.gene72238.t1 AT3G25050 44.922 256 132 7 3 255 41 290 4.72e-74 228
MS.gene72238.t1 AT1G11545 43.243 259 137 5 3 254 44 299 2.21e-73 226
MS.gene72238.t1 AT3G44990 39.147 258 138 7 5 254 47 293 2.31e-58 187
MS.gene72238.t1 AT2G36870 40.000 260 137 7 5 256 51 299 4.02e-57 184
MS.gene72238.t1 AT1G10550 34.866 261 157 5 5 254 51 309 6.03e-50 166
MS.gene72238.t1 AT3G48580 37.008 254 140 9 2 254 39 273 5.65e-48 160
MS.gene72238.t1 AT1G32170 35.878 262 148 10 5 254 40 293 5.48e-47 160
MS.gene72238.t1 AT1G14720 34.363 259 154 7 5 254 39 290 1.38e-45 155
MS.gene72238.t1 AT2G01850 33.333 246 154 5 14 254 50 290 1.50e-45 155
MS.gene72238.t1 AT4G18990 33.708 267 158 6 5 254 48 312 3.72e-45 155
MS.gene72238.t1 AT3G48580 35.652 230 129 8 25 254 4 214 2.84e-41 141
MS.gene72238.t1 AT4G18990 35.323 201 116 4 66 254 40 238 8.15e-39 137
MS.gene72238.t1 AT2G36870 48.438 128 58 2 5 124 51 178 1.69e-33 120
MS.gene72238.t1 AT3G48580 33.071 254 130 10 2 254 39 253 6.71e-33 120

Find 61 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTCTAAAATGATTGAATT+TGG 0.145467 6.3:-81580354 None:intergenic
TGAAAGTTCACACAAGTTTA+TGG 0.173797 6.3:+81580264 MS.gene72238:CDS
GTTTATGGAATGCAGATAAT+TGG 0.180578 6.3:+81580279 MS.gene72238:CDS
ACAAAGAAACGATTCTTATT+TGG 0.190589 6.3:+81578856 MS.gene72238:CDS
CGTGATACCAAAAGATTTAA+TGG 0.220099 6.3:+81580533 MS.gene72238:CDS
CTCAATTAGGTCAAATGAAT+TGG 0.230910 6.3:+81580477 MS.gene72238:CDS
TAGTTTCTGAATACCCTAAT+TGG 0.268420 6.3:-81580203 None:intergenic
CATTTGACCATTAAATCTTT+TGG 0.275194 6.3:-81580540 None:intergenic
CTTTCATTCCTTGCTTGTTT+GGG 0.284686 6.3:-81580247 None:intergenic
ACTTTCATTCCTTGCTTGTT+TGG 0.288073 6.3:-81580248 None:intergenic
TTAGAGCAAGAGCTTGTAAA+TGG 0.290852 6.3:+81580369 MS.gene72238:CDS
AATTGTGCCTTGAAACATTC+AGG 0.294572 6.3:-81580566 None:intergenic
ATATTGATAGTATACCAATT+AGG 0.297472 6.3:+81580189 MS.gene72238:CDS
GTGGACTTGTTAAGACAAAT+TGG 0.298616 6.3:+81580312 MS.gene72238:CDS
TTCTCCTTCATACAAACAAT+TGG 0.316925 6.3:+81580448 MS.gene72238:CDS
TGCTCAACATGCAATACTTC+AGG 0.322813 6.3:+81578254 MS.gene72238:CDS
GAGTTCTTGGGAAATAGTTC+TGG 0.332738 6.3:+81579138 MS.gene72238:CDS
CAATTGGGTTATGCTCAATT+AGG 0.347787 6.3:+81580464 MS.gene72238:CDS
CAATTTGATTCGTGATTCAA+TGG 0.364662 6.3:-81578881 None:intergenic
TTTAGTTGAACTTACATAGT+AGG 0.375117 6.3:-81578935 None:intergenic
CATTGAATCACGAATCAAAT+TGG 0.377433 6.3:+81578882 MS.gene72238:CDS
CTTTAAATATAGCTGCAAAT+TGG 0.393492 6.3:+81580420 MS.gene72238:CDS
ACTGTTCCTGCAGAATTTCC+AGG 0.399025 6.3:-81578907 None:intergenic
AATGTATACACACAAGGAAA+TGG 0.402004 6.3:+81579183 MS.gene72238:CDS
CTCATCATGTTGGCTTCCTA+TGG 0.404211 6.3:-81579106 None:intergenic
TATTGATAGTATACCAATTA+GGG 0.404217 6.3:+81580190 MS.gene72238:CDS
CTTGTGCTGGATAAAACTTC+AGG 0.420068 6.3:+81578291 MS.gene72238:CDS
TTTATGGAATGCAGATAATT+GGG 0.420505 6.3:+81580280 MS.gene72238:CDS
ACATTTGTATGAACAATGTA+AGG 0.437791 6.3:-81579165 None:intergenic
CCAAAAGCATGTATATTACA+TGG 0.438084 6.3:+81578229 None:intergenic
TAACCCAATTGTTTGTATGA+AGG 0.450345 6.3:-81580452 None:intergenic
AATGCCTCCTGAATGTTTCA+AGG 0.456192 6.3:+81580559 MS.gene72238:CDS
ATGAACTTACACAATTTCAG+TGG 0.460781 6.3:-81579280 None:intergenic
TTCATAACTATACTATCCAT+TGG 0.462357 6.3:+81579253 MS.gene72238:CDS
TCTCCTTCATACAAACAATT+GGG 0.471417 6.3:+81580449 MS.gene72238:CDS
CTTAACAAGTCCACCTCTTG+TGG 0.471870 6.3:-81580303 None:intergenic
TCAATTAGGTCAAATGAATT+GGG 0.474336 6.3:+81580478 MS.gene72238:CDS
TGAAGATCTTCAACTTGTGC+TGG 0.478258 6.3:+81578278 MS.gene72238:CDS
TAAATATAGCTGCAAATTGG+TGG 0.492166 6.3:+81580423 MS.gene72238:CDS
ATTCAGAAACTATGAAAATG+AGG 0.505200 6.3:+81580214 MS.gene72238:CDS
CAATTTCAGTGGGGTTCCAA+TGG 0.505427 6.3:-81579269 None:intergenic
TGATTGAATTTGGCTACAAA+TGG 0.508974 6.3:-81580344 None:intergenic
TAGTTATGAAAATCAGCAGT+TGG 0.519542 6.3:-81579240 None:intergenic
TTGGTCCCTGGAAATTCTGC+AGG 0.523041 6.3:+81578901 MS.gene72238:CDS
TGAACTTACACAATTTCAGT+GGG 0.537978 6.3:-81579279 None:intergenic
TCACGAATCAAATTGGTCCC+TGG 0.544615 6.3:+81578889 MS.gene72238:CDS
GATAATTGGGCCACAAGAGG+TGG 0.546524 6.3:+81580293 MS.gene72238:CDS
AGTTATGAAAATCAGCAGTT+GGG 0.554006 6.3:-81579239 None:intergenic
GCTCAACATGCAATACTTCA+GGG 0.554741 6.3:+81578255 MS.gene72238:CDS
CAAAAGCATGTATATTACAT+GGG 0.559154 6.3:+81578230 None:intergenic
GCAAGAGCTTGTAAATGGAA+TGG 0.563028 6.3:+81580374 MS.gene72238:CDS
CAAAATCTATCTCATCATGT+TGG 0.568146 6.3:-81579116 None:intergenic
TTCAGAAACTATGAAAATGA+GGG 0.605928 6.3:+81580215 MS.gene72238:CDS
CTGTTCCTGCAGAATTTCCA+GGG 0.607228 6.3:-81578906 None:intergenic
TGTGTGCAGCTATCTTCCAT+AGG 0.608092 6.3:+81579090 MS.gene72238:intron
GCAGATAATTGGGCCACAAG+AGG 0.610239 6.3:+81580290 MS.gene72238:CDS
ATTGCATACCCAAACAAGCA+AGG 0.626861 6.3:+81580239 MS.gene72238:CDS
CATACAAATGTATACACACA+AGG 0.631731 6.3:+81579177 MS.gene72238:CDS
AAAAGCATGTATATTACATG+GGG 0.648450 6.3:+81578231 None:intergenic
TGTGCCTTGAAACATTCAGG+AGG 0.693280 6.3:-81580563 None:intergenic
GAACTTACACAATTTCAGTG+GGG 0.729780 6.3:-81579278 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAATTTTTATATCATTAG+AGG + chr6.3:81579389-81579408 MS.gene72238:intron 10.0%
!!! TTTTTTTATTAATCAAACAT+AGG + chr6.3:81578522-81578541 MS.gene72238:intron 10.0%
!! TGATTTATAAAACATTAGAA+AGG - chr6.3:81580133-81580152 None:intergenic 15.0%
!!! AAAATTCACATTGTTATTAT+CGG - chr6.3:81578716-81578735 None:intergenic 15.0%
!!! TTCTAATGTTTTATAAATCA+TGG + chr6.3:81580133-81580152 MS.gene72238:intron 15.0%
!! AAAAATCTTAAGAATGATAG+TGG - chr6.3:81579350-81579369 None:intergenic 20.0%
!! ACTTTCTTATAGAAATGAAA+AGG - chr6.3:81580100-81580119 None:intergenic 20.0%
!! ATATACTCTCCTATATAAAA+TGG + chr6.3:81579662-81579681 MS.gene72238:intron 20.0%
!! CTTTCTTATAGAAATGAAAA+GGG - chr6.3:81580099-81580118 None:intergenic 20.0%
!! TGAAAAAATTCTCGTTAAAT+TGG - chr6.3:81579717-81579736 None:intergenic 20.0%
!!! AAATTCACATTGTTATTATC+GGG - chr6.3:81578715-81578734 None:intergenic 20.0%
!!! ATATTGATAGTATACCAATT+AGG + chr6.3:81580189-81580208 MS.gene72238:CDS 20.0%
!!! TATTGATAGTATACCAATTA+GGG + chr6.3:81580190-81580209 MS.gene72238:CDS 20.0%
!!! TATTTGAAATCTGTACATTA+AGG - chr6.3:81579540-81579559 None:intergenic 20.0%
!!! TCATTTCTATAAGAAAGTAT+TGG + chr6.3:81580102-81580121 MS.gene72238:intron 20.0%
!!! TGAAAATGAGATTTTACATA+AGG + chr6.3:81579033-81579052 MS.gene72238:intron 20.0%
! AATCTCTAAATAGCTAAATG+AGG + chr6.3:81579752-81579771 MS.gene72238:intron 25.0%
! ACAAAGAAACGATTCTTATT+TGG + chr6.3:81578856-81578875 MS.gene72238:CDS 25.0%
! ACATTTGTATGAACAATGTA+AGG - chr6.3:81579168-81579187 None:intergenic 25.0%
! ATTCAGAAACTATGAAAATG+AGG + chr6.3:81580214-81580233 MS.gene72238:CDS 25.0%
! CTTTAAATATAGCTGCAAAT+TGG + chr6.3:81580420-81580439 MS.gene72238:CDS 25.0%
! TCAACTTAACATGTAAGATA+TGG + chr6.3:81578797-81578816 MS.gene72238:intron 25.0%
! TCAATTAGGTCAAATGAATT+GGG + chr6.3:81580478-81580497 MS.gene72238:CDS 25.0%
! TCTTTGAATATAAATTCACG+AGG + chr6.3:81579481-81579500 MS.gene72238:intron 25.0%
! TTATAAATCATGGCTTACAT+TGG + chr6.3:81580143-81580162 MS.gene72238:intron 25.0%
! TTCAGAAACTATGAAAATGA+GGG + chr6.3:81580215-81580234 MS.gene72238:CDS 25.0%
! TTCATAACTATACTATCCAT+TGG + chr6.3:81579253-81579272 MS.gene72238:CDS 25.0%
! TTTAGTTGAACTTACATAGT+AGG - chr6.3:81578938-81578957 None:intergenic 25.0%
! TTTATGGAATGCAGATAATT+GGG + chr6.3:81580280-81580299 MS.gene72238:CDS 25.0%
!! CATTTGACCATTAAATCTTT+TGG - chr6.3:81580543-81580562 None:intergenic 25.0%
!! GTTTATGTATACAAGCTTTT+TGG + chr6.3:81579441-81579460 MS.gene72238:intron 25.0%
!! TAGGAGAGTATATTTAACTA+TGG - chr6.3:81579655-81579674 None:intergenic 25.0%
!! TTAGTGTGTCCATTTTATAT+AGG - chr6.3:81579674-81579693 None:intergenic 25.0%
!!! TGCTCTAAAATGATTGAATT+TGG - chr6.3:81580357-81580376 None:intergenic 25.0%
!!! TTGGTTTTTTTTTTTTCCAC+AGG + chr6.3:81580162-81580181 MS.gene72238:intron 25.0%
AAACTAAACAGGCACTAAAT+CGG - chr6.3:81579784-81579803 None:intergenic 30.0%
AATGTATACACACAAGGAAA+TGG + chr6.3:81579183-81579202 MS.gene72238:CDS 30.0%
ACTTAACATGTAAGATATGG+TGG + chr6.3:81578800-81578819 MS.gene72238:intron 30.0%
AGTTATGAAAATCAGCAGTT+GGG - chr6.3:81579242-81579261 None:intergenic 30.0%
ATGAACTTACACAATTTCAG+TGG - chr6.3:81579283-81579302 None:intergenic 30.0%
ATGGTGCATCTTTCAATATT+TGG + chr6.3:81578455-81578474 MS.gene72238:intron 30.0%
CAAAATCTATCTCATCATGT+TGG - chr6.3:81579119-81579138 None:intergenic 30.0%
CATACAAATGTATACACACA+AGG + chr6.3:81579177-81579196 MS.gene72238:CDS 30.0%
CATTGAATCACGAATCAAAT+TGG + chr6.3:81578882-81578901 MS.gene72238:CDS 30.0%
CGTGATACCAAAAGATTTAA+TGG + chr6.3:81580533-81580552 MS.gene72238:CDS 30.0%
CTCAATTAGGTCAAATGAAT+TGG + chr6.3:81580477-81580496 MS.gene72238:CDS 30.0%
GATTAAGTCGATTGTTCTAA+TGG - chr6.3:81578991-81579010 None:intergenic 30.0%
GTTTATGGAATGCAGATAAT+TGG + chr6.3:81580279-81580298 MS.gene72238:CDS 30.0%
TAAATATAGCTGCAAATTGG+TGG + chr6.3:81580423-81580442 MS.gene72238:CDS 30.0%
TAACCCAATTGTTTGTATGA+AGG - chr6.3:81580455-81580474 None:intergenic 30.0%
TAATTACTGATAAGTAGCCA+AGG - chr6.3:81578683-81578702 None:intergenic 30.0%
TAGTTATGAAAATCAGCAGT+TGG - chr6.3:81579243-81579262 None:intergenic 30.0%
TAGTTGTAACTTTGTAAGCA+TGG + chr6.3:81578436-81578455 MS.gene72238:intron 30.0%
TAGTTTCTGAATACCCTAAT+TGG - chr6.3:81580206-81580225 None:intergenic 30.0%
TCTCCTTCATACAAACAATT+GGG + chr6.3:81580449-81580468 MS.gene72238:CDS 30.0%
TGAAAGTTCACACAAGTTTA+TGG + chr6.3:81580264-81580283 MS.gene72238:CDS 30.0%
TGAACTTACACAATTTCAGT+GGG - chr6.3:81579282-81579301 None:intergenic 30.0%
TGATTGAATTTGGCTACAAA+TGG - chr6.3:81580347-81580366 None:intergenic 30.0%
TTCTCCTTCATACAAACAAT+TGG + chr6.3:81580448-81580467 MS.gene72238:CDS 30.0%
! AAGCTAACACTTGAAGTTTT+TGG + chr6.3:81579867-81579886 MS.gene72238:intron 30.0%
! AGCTAACACTTGAAGTTTTT+GGG + chr6.3:81579868-81579887 MS.gene72238:intron 30.0%
! CAATTTGATTCGTGATTCAA+TGG - chr6.3:81578884-81578903 None:intergenic 30.0%
! GAGAGCAACAATTTTATCTT+TGG + chr6.3:81579211-81579230 MS.gene72238:CDS 30.0%
!!! GAGATAGATTTTGAGTTCTT+GGG + chr6.3:81579126-81579145 MS.gene72238:CDS 30.0%
!!! GTTTTTTTTTTTTCCACAGG+TGG + chr6.3:81580165-81580184 MS.gene72238:intron 30.0%
!!! TGAGATAGATTTTGAGTTCT+TGG + chr6.3:81579125-81579144 MS.gene72238:CDS 30.0%
AAATCATAACGTGGGATAGT+GGG + chr6.3:81578582-81578601 MS.gene72238:intron 35.0%
AATTGTGCCTTGAAACATTC+AGG - chr6.3:81580569-81580588 None:intergenic 35.0%
ACTATCCCACGTTATGATTT+AGG - chr6.3:81578582-81578601 None:intergenic 35.0%
ACTTTCATTCCTTGCTTGTT+TGG - chr6.3:81580251-81580270 None:intergenic 35.0%
ATTGGGTCAAGCATGAAAAA+CGG + chr6.3:81578645-81578664 MS.gene72238:intron 35.0%
CAATTGGGTTATGCTCAATT+AGG + chr6.3:81580464-81580483 MS.gene72238:CDS 35.0%
CTATCCCACGTTATGATTTA+GGG - chr6.3:81578581-81578600 None:intergenic 35.0%
CTTTCATTCCTTGCTTGTTT+GGG - chr6.3:81580250-81580269 None:intergenic 35.0%
GAACTTACACAATTTCAGTG+GGG - chr6.3:81579281-81579300 None:intergenic 35.0%
GATTTAGTGCCTGTTTAGTT+TGG + chr6.3:81579783-81579802 MS.gene72238:intron 35.0%
TAAATCATAACGTGGGATAG+TGG + chr6.3:81578581-81578600 MS.gene72238:intron 35.0%
TACTATCAATATACCACCTG+TGG - chr6.3:81580181-81580200 None:intergenic 35.0%
TATCCCACGTTATGATTTAG+GGG - chr6.3:81578580-81578599 None:intergenic 35.0%
TCACAACCCAATAAACGATT+GGG + chr6.3:81578628-81578647 MS.gene72238:intron 35.0%
TTAGAGCAAGAGCTTGTAAA+TGG + chr6.3:81580369-81580388 MS.gene72238:CDS 35.0%
TTGCAAACTCCAAACTAAAC+AGG - chr6.3:81579795-81579814 None:intergenic 35.0%
! CCACGTTTTCTATAAAACCA+CGG - chr6.3:81579910-81579929 None:intergenic 35.0%
!! GTGGACTTGTTAAGACAAAT+TGG + chr6.3:81580312-81580331 MS.gene72238:CDS 35.0%
!! TGGTGTTACATTTGAATTGC+AGG + chr6.3:81578820-81578839 MS.gene72238:intron 35.0%
!!! TTGAAGTTTTTGGGTTAACG+TGG + chr6.3:81579877-81579896 MS.gene72238:intron 35.0%
AATGCCTCCTGAATGTTTCA+AGG + chr6.3:81580559-81580578 MS.gene72238:CDS 40.0%
ACTACCCCTAAATCATAACG+TGG + chr6.3:81578573-81578592 MS.gene72238:intron 40.0%
ATTGCATACCCAAACAAGCA+AGG + chr6.3:81580239-81580258 MS.gene72238:CDS 40.0%
CTACCCCTAAATCATAACGT+GGG + chr6.3:81578574-81578593 MS.gene72238:intron 40.0%
CTTGTGCTGGATAAAACTTC+AGG + chr6.3:81578291-81578310 MS.gene72238:CDS 40.0%
GAGTTCTTGGGAAATAGTTC+TGG + chr6.3:81579138-81579157 MS.gene72238:CDS 40.0%
GCAAGAGCTTGTAAATGGAA+TGG + chr6.3:81580374-81580393 MS.gene72238:CDS 40.0%
GCTCAACATGCAATACTTCA+GGG + chr6.3:81578255-81578274 MS.gene72238:CDS 40.0%
GCTTGACCCAATCGTTTATT+GGG - chr6.3:81578637-81578656 None:intergenic 40.0%
GTCACAACCCAATAAACGAT+TGG + chr6.3:81578627-81578646 MS.gene72238:intron 40.0%
TGAAGATCTTCAACTTGTGC+TGG + chr6.3:81578278-81578297 MS.gene72238:CDS 40.0%
TGCTCAACATGCAATACTTC+AGG + chr6.3:81578254-81578273 MS.gene72238:CDS 40.0%
TGCTTGACCCAATCGTTTAT+TGG - chr6.3:81578638-81578657 None:intergenic 40.0%
! ATCGTTTATTGGGTTGTGAC+CGG - chr6.3:81578627-81578646 None:intergenic 40.0%
!!! CCGTGGTTTTATAGAAAACG+TGG + chr6.3:81579907-81579926 MS.gene72238:intron 40.0%
AACCACAAACGCACTTATGC+AGG + chr6.3:81579813-81579832 MS.gene72238:intron 45.0%
ACTGTTCCTGCAGAATTTCC+AGG - chr6.3:81578910-81578929 None:intergenic 45.0%
ATCCTGCATAAGTGCGTTTG+TGG - chr6.3:81579818-81579837 None:intergenic 45.0%
CAATTTCAGTGGGGTTCCAA+TGG - chr6.3:81579272-81579291 None:intergenic 45.0%
CTGTTCCTGCAGAATTTCCA+GGG - chr6.3:81578909-81578928 None:intergenic 45.0%
CTTAACAAGTCCACCTCTTG+TGG - chr6.3:81580306-81580325 None:intergenic 45.0%
TCACGAATCAAATTGGTCCC+TGG + chr6.3:81578889-81578908 MS.gene72238:CDS 45.0%
TGTGCCTTGAAACATTCAGG+AGG - chr6.3:81580566-81580585 None:intergenic 45.0%
TGTGTGCAGCTATCTTCCAT+AGG + chr6.3:81579090-81579109 MS.gene72238:intron 45.0%
! TCGTTTATTGGGTTGTGACC+GGG - chr6.3:81578626-81578645 None:intergenic 45.0%
!! CTCATCATGTTGGCTTCCTA+TGG - chr6.3:81579109-81579128 None:intergenic 45.0%
!!! TTTAAATTTATGTTATTTAT+TGG - chr6.3:81578965-81578984 None:intergenic 5.0%
GATAATTGGGCCACAAGAGG+TGG + chr6.3:81580293-81580312 MS.gene72238:CDS 50.0%
GCAGATAATTGGGCCACAAG+AGG + chr6.3:81580290-81580309 MS.gene72238:CDS 50.0%
GTTAACGTGGTGAGTTACCG+TGG + chr6.3:81579890-81579909 MS.gene72238:intron 50.0%
TTGGTCCCTGGAAATTCTGC+AGG + chr6.3:81578901-81578920 MS.gene72238:CDS 50.0%
! CGTTTATTGGGTTGTGACCG+GGG - chr6.3:81578625-81578644 None:intergenic 50.0%
!! AACGGTCGCTCTAGAAACCT+TGG + chr6.3:81578663-81578682 MS.gene72238:intron 50.0%
!! TCATAACGTGGGATAGTGGG+AGG + chr6.3:81578585-81578604 MS.gene72238:intron 50.0%
! ATTGGGTTGTGACCGGGGTG+TGG - chr6.3:81578620-81578639 None:intergenic 60.0%
! TTGGGTTGTGACCGGGGTGT+GGG - chr6.3:81578619-81578638 None:intergenic 60.0%
AGGTTTCACCCCCCACACCC+CGG + chr6.3:81578605-81578624 MS.gene72238:intron 65.0%
TGGGTTGTGACCGGGGTGTG+GGG - chr6.3:81578618-81578637 None:intergenic 65.0%
GGGTTGTGACCGGGGTGTGG+GGG - chr6.3:81578617-81578636 None:intergenic 70.0%
GGTTGTGACCGGGGTGTGGG+GGG - chr6.3:81578616-81578635 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 81578237 81580592 81578237 ID=MS.gene72238
chr6.3 mRNA 81578237 81580592 81578237 ID=MS.gene72238.t1;Parent=MS.gene72238
chr6.3 exon 81578237 81578312 81578237 ID=MS.gene72238.t1.exon1;Parent=MS.gene72238.t1
chr6.3 CDS 81578237 81578312 81578237 ID=cds.MS.gene72238.t1;Parent=MS.gene72238.t1
chr6.3 exon 81578842 81578942 81578842 ID=MS.gene72238.t1.exon2;Parent=MS.gene72238.t1
chr6.3 CDS 81578842 81578942 81578842 ID=cds.MS.gene72238.t1;Parent=MS.gene72238.t1
chr6.3 exon 81579099 81579292 81579099 ID=MS.gene72238.t1.exon3;Parent=MS.gene72238.t1
chr6.3 CDS 81579099 81579292 81579099 ID=cds.MS.gene72238.t1;Parent=MS.gene72238.t1
chr6.3 exon 81580184 81580592 81580184 ID=MS.gene72238.t1.exon4;Parent=MS.gene72238.t1
chr6.3 CDS 81580184 81580592 81580184 ID=cds.MS.gene72238.t1;Parent=MS.gene72238.t1
Gene Sequence

>MS.gene72238

ATGTATATTACATGGGGTGCTCAACATGCAATACTTCAGGGTGAAGATCTTCAACTTGTGCTGGATAAAACTTCAGGTAGAATTAAATTTTGTACTTATAACTTGCATCAAATTTAATTAGATTATTTAATAAAAATATTAATTAGATTATATGTTTGTCTTTGTAATACATTAAATTTTTTATAAAGAAGATAATTTATAGTTGTAACTTTGTAAGCATGGTGCATCTTTCAATATTTGGCAATTGAATGTCAAATAAATGAAAAAAAAATTGTGATTCAAACTTTTTTTTATTAATCAAACATAGGTTTTTTCATTGACTATTCAATCTACAATACTACCCCTAAATCATAACGTGGGATAGTGGGAGGTTTCACCCCCCACACCCCGGTCACAACCCAATAAACGATTGGGTCAAGCATGAAAAACGGTCGCTCTAGAAACCTTGGCTACTTATCAGTAATTATGAATCATACCCGATAATAACAATGTGAATTTTTCTTTAATCAATGTGTTAATTATATATTTGAATATAAATAAGCAAATGAATTTTTCTTGTGTCAACTTAACATGTAAGATATGGTGGTGTTACATTTGAATTGCAGGATCTGCAGCAGAAACAAAGAAACGATTCTTATTTGGAACCATTGAATCACGAATCAAATTGGTCCCTGGAAATTCTGCAGGAACAGTTACATCCTACTATGTAAGTTCAACTAAACGATCCAATAAATAACATAAATTTAAATTTCCATTAGAACAATCGACTTAATCTCATATTATATTAATAAAAATGTGAAAATGAGATTTTACATAAGGTACAAAATATGCTAATGTTTCATTTTCATAAAAATGTGTGCAGCTATCTTCCATAGGAAGCCAACATGATGAGATAGATTTTGAGTTCTTGGGAAATAGTTCTGGACAACCTTACATTGTTCATACAAATGTATACACACAAGGAAATGGAGATAGAGAGCAACAATTTTATCTTTGGTTTGACCCAACTGCTGATTTTCATAACTATACTATCCATTGGAACCCCACTGAAATTGTGTAAGTTCATAAAATACTAAACTCTACTAAGCATTCATTAAGTTTAATTTTAATCCACTATCATTCTTAAGATTTTTAGTATACTATCAACTAATCTTAAATTTTTATATCATTAGAGGTGTTGAATTTAATATTAATTTCTACATTTGTTTATGTATACAAGCTTTTTGGAAGAAAAAAAAAAATCGTCTTTGAATATAAATTCACGAGGTGTCAACTTCGTGATAAGAGTTTGACTCTATAACCTTAATGTACAGATTTCAAATATCATATGATATTGTTGTAAAATGATTATTAATGATTCGCATTTCAAATTTTATATATGATTATTATTTTTAAGTGTTTTGAAAGATGAAAATCCATAGTTAAATATACTCTCCTATATAAAATGGACACACTAATCATTGTAAGCTTCTAACGACCAATTTAACGAGAATTTTTTCAATTCATTGCGAAAGCAATCTCTAAATAGCTAAATGAGGTTGTGTCCGATTTAGTGCCTGTTTAGTTTGGAGTTTGCAACCACAAACGCACTTATGCAGGATTTTCAAAGCAAAACTGTTGTGATTTCTAAAAGCTAACACTTGAAGTTTTTGGGTTAACGTGGTGAGTTACCGTGGTTTTATAGAAAACGTGGTTTGCAAAACGCAGAAGCAAACAAGCACTTAGAGATTGATTTGCAAAAAGTCATTAAAAATACTACTCATTATGTTAAAATTACAAAATAAGTACTTAAAAATCACATAAAACTATTTGTGATCAGTTGACATTGTAAAATATTATTTTGATAGTGATATGATTTTCCCTTTTCATTTCTATAAGAAAGTATTGGTTACACCTTTCTAATGTTTTATAAATCATGGCTTACATTGGTTTTTTTTTTTTCCACAGGTGGTATATTGATAGTATACCAATTAGGGTATTCAGAAACTATGAAAATGAGGGCATTGCATACCCAAACAAGCAAGGAATGAAAGTTCACACAAGTTTATGGAATGCAGATAATTGGGCCACAAGAGGTGGACTTGTTAAGACAAATTGGACTAATGCACCATTTGTAGCCAAATTCAATCATTTTAGAGCAAGAGCTTGTAAATGGAATGGAGCAATTAGTATTAATCAATGTGCTTTAAATATAGCTGCAAATTGGTGGACTTCTCCTTCATACAAACAATTGGGTTATGCTCAATTAGGTCAAATGAATTGGGTCAGAAATAATTATATGATCTATGATTATTGTCGTGATACCAAAAGATTTAATGGTCAAATGCCTCCTGAATGTTTCAAGGCACAATTCTAA
Protein sequence

>MS.gene72238.t1

MYITWGAQHAILQGEDLQLVLDKTSGSAAETKKRFLFGTIESRIKLVPGNSAGTVTSYYLSSIGSQHDEIDFEFLGNSSGQPYIVHTNVYTQGNGDREQQFYLWFDPTADFHNYTIHWNPTEIVWYIDSIPIRVFRNYENEGIAYPNKQGMKVHTSLWNADNWATRGGLVKTNWTNAPFVAKFNHFRARACKWNGAISINQCALNIAANWWTSPSYKQLGYAQLGQMNWVRNNYMIYDYCRDTKRFNGQMPPECFKAQF