Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72253.t1 | XP_013453115.1 | 90.6 | 244 | 22 | 1 | 1 | 243 | 78 | 321 | 4.80E-117 | 430.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72253.t1 | Q8S3D1 | 64.9 | 194 | 56 | 5 | 64 | 245 | 213 | 406 | 1.0e-59 | 231.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72253.t1 | I3S6F8 | 90.6 | 244 | 22 | 1 | 1 | 243 | 78 | 321 | 3.4e-117 | 430.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050680 | MS.gene72253 | 0.804592 | 2.01E-49 | -1.69E-46 |
| MS.gene050750 | MS.gene72253 | 0.80638 | 8.46E-50 | -1.69E-46 |
| MS.gene051568 | MS.gene72253 | 0.813472 | 2.52E-51 | -1.69E-46 |
| MS.gene052333 | MS.gene72253 | 0.81951 | 1.12E-52 | -1.69E-46 |
| MS.gene05288 | MS.gene72253 | 0.805649 | 1.21E-49 | -1.69E-46 |
| MS.gene053166 | MS.gene72253 | 0.820749 | 5.82E-53 | -1.69E-46 |
| MS.gene053494 | MS.gene72253 | 0.822144 | 2.77E-53 | -1.69E-46 |
| MS.gene053584 | MS.gene72253 | 0.813213 | 2.87E-51 | -1.69E-46 |
| MS.gene054183 | MS.gene72253 | 0.822033 | 2.94E-53 | -1.69E-46 |
| MS.gene054433 | MS.gene72253 | 0.800724 | 1.26E-48 | -1.69E-46 |
| MS.gene054559 | MS.gene72253 | 0.838432 | 2.89E-57 | -1.69E-46 |
| MS.gene05510 | MS.gene72253 | 0.803623 | 3.19E-49 | -1.69E-46 |
| MS.gene055356 | MS.gene72253 | 0.80971 | 1.65E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene72253 | 0.839714 | 1.34E-57 | -1.69E-46 |
| MS.gene059609 | MS.gene72253 | 0.880408 | 5.63E-70 | -1.69E-46 |
| MS.gene061114 | MS.gene72253 | -0.808755 | 2.65E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 67 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTACAGCTCCTAAATGAAAA+TGG | 0.255266 | 6.3:-81310268 | MS.gene72253:intron |
| CTACTCTTTAGGTCCTTCTT+TGG | 0.274553 | 6.3:+81309922 | None:intergenic |
| ATGACAATGGAGCTGATTAT+TGG | 0.285895 | 6.3:-81309855 | MS.gene72253:CDS |
| CATCAGCTTGTTTCCCCATT+TGG | 0.289480 | 6.3:-81312828 | MS.gene72253:CDS |
| ATTACCTGACCAGGGTCTTC+AGG | 0.299545 | 6.3:+81310494 | None:intergenic |
| CCTGATCCATTGTCCAAGTA+TGG | 0.317233 | 6.3:+81311805 | None:intergenic |
| GGCTCTTAGCTTACCATACT+TGG | 0.320593 | 6.3:-81311818 | MS.gene72253:CDS |
| TGGTTATATCAGATTCCTTC+AGG | 0.336279 | 6.3:-81311997 | MS.gene72253:CDS |
| CAGGTTAGGAAGGAGAAGTT+AGG | 0.364935 | 6.3:-81312870 | MS.gene72253:CDS |
| TCTGTCTTGTTAGAAGCTAT+TGG | 0.377963 | 6.3:-81312017 | MS.gene72253:CDS |
| AATTAATTACCTTTCCAAAT+GGG | 0.386503 | 6.3:+81312814 | None:intergenic |
| ACATCAACAAGAGAAGCATT+TGG | 0.400442 | 6.3:+81317205 | None:intergenic |
| ACTTGTAGTGTACATGATAC+TGG | 0.401193 | 6.3:+81309883 | None:intergenic |
| TGACAATGGAGCTGATTATT+GGG | 0.408868 | 6.3:-81309854 | MS.gene72253:CDS |
| CCCTAGGAGGAGGAGGCTTT+CGG | 0.409114 | 6.3:-81309825 | MS.gene72253:CDS |
| TACAACTCAAACTACTTTCA+AGG | 0.412851 | 6.3:-81313166 | MS.gene72253:intron |
| TTTAATCTTAATTAGGTTCA+AGG | 0.413424 | 6.3:-81310536 | MS.gene72253:intron |
| GACACAACCCTCTACTCTTT+AGG | 0.414717 | 6.3:+81309911 | None:intergenic |
| TAATTAATTACCTTTCCAAA+TGG | 0.416474 | 6.3:+81312813 | None:intergenic |
| AATTGAAGGGGTGGAATTGT+GGG | 0.425149 | 6.3:-81317286 | None:intergenic |
| TAATTGAAGGGGTGGAATTG+TGG | 0.447002 | 6.3:-81317287 | None:intergenic |
| GCACCTGCCCTAGGAGGAGG+AGG | 0.461982 | 6.3:-81309832 | MS.gene72253:CDS |
| AAACACAAGGCAACAACATC+CGG | 0.472522 | 6.3:-81311782 | MS.gene72253:intron |
| TTACCTGACCAGGGTCTTCA+GGG | 0.475060 | 6.3:+81310495 | None:intergenic |
| ACCGAAAGCCTCCTCCTCCT+AGG | 0.478830 | 6.3:+81309824 | None:intergenic |
| ATTAATTACCTTTCCAAATG+GGG | 0.483813 | 6.3:+81312815 | None:intergenic |
| AAGAAGGACCTAAAGAGTAG+AGG | 0.491449 | 6.3:-81309919 | MS.gene72253:CDS |
| ATTCCTTCAGGCTCAAATTG+AGG | 0.494505 | 6.3:-81311985 | MS.gene72253:intron |
| AAGTGCAACAGTACTACATC+TGG | 0.495170 | 6.3:-81313222 | MS.gene72253:CDS |
| CCATACTTGGACAATGGATC+AGG | 0.498280 | 6.3:-81311805 | MS.gene72253:CDS |
| TCAGGAAATAGCTATGCATA+TGG | 0.501689 | 6.3:-81317169 | MS.gene72253:CDS |
| ATTCCCTGAAGACCCTGGTC+AGG | 0.501966 | 6.3:-81310498 | MS.gene72253:intron |
| AGCTTACCATACTTGGACAA+TGG | 0.504007 | 6.3:-81311811 | MS.gene72253:CDS |
| TTCTCAATTTCAGGTTAGGA+AGG | 0.504978 | 6.3:-81312880 | MS.gene72253:intron |
| GATTATTGGGCACCTGCCCT+AGG | 0.505746 | 6.3:-81309841 | MS.gene72253:CDS |
| TTTCAACAACAACAACATGT+TGG | 0.505959 | 6.3:-81317110 | MS.gene72253:CDS |
| AGAAGGACCTAAAGAGTAGA+GGG | 0.506332 | 6.3:-81309918 | MS.gene72253:CDS |
| ATGGGGAAACAAGCTGATGA+AGG | 0.508848 | 6.3:+81312832 | None:intergenic |
| AAGGGGTGGAATTGTGGGAG+AGG | 0.513254 | 6.3:-81317281 | MS.gene72253:CDS |
| TGGGCACCTGCCCTAGGAGG+AGG | 0.520166 | 6.3:-81309835 | MS.gene72253:CDS |
| GTGGGAGAGGAAGATAAGAT+TGG | 0.524644 | 6.3:-81317268 | MS.gene72253:CDS |
| TATCATGTACACTACAAGTT+GGG | 0.530955 | 6.3:-81309879 | MS.gene72253:CDS |
| ATCCAAAGGATTACCTGACC+AGG | 0.531475 | 6.3:+81310485 | None:intergenic |
| GATGTTAAACAAGAAAGCTC+AGG | 0.533379 | 6.3:-81317187 | MS.gene72253:CDS |
| AAGCCTCCTCCTCCTAGGGC+AGG | 0.533559 | 6.3:+81309829 | None:intergenic |
| TTCTCAAGAAGAACCAAAGA+AGG | 0.544458 | 6.3:-81309935 | MS.gene72253:CDS |
| CCGAAAGCCTCCTCCTCCTA+GGG | 0.547448 | 6.3:+81309825 | None:intergenic |
| GAAGAGGAAAGCAGCTGAAG+AGG | 0.554156 | 6.3:-81310242 | MS.gene72253:intron |
| TGCAACAGTACTACATCTGG+TGG | 0.567465 | 6.3:-81313219 | MS.gene72253:CDS |
| GCTTGTTTCCCCATTTGGAA+AGG | 0.570374 | 6.3:-81312823 | MS.gene72253:intron |
| TGTTTATTCCCTGAAGACCC+TGG | 0.579599 | 6.3:-81310503 | MS.gene72253:CDS |
| TCACCTCAATTTGAGCCTGA+AGG | 0.591976 | 6.3:+81311982 | None:intergenic |
| TAAATGAAAATGGTTTGAAG+AGG | 0.595062 | 6.3:-81310258 | MS.gene72253:CDS |
| GTATCATGTACACTACAAGT+TGG | 0.599973 | 6.3:-81309880 | MS.gene72253:CDS |
| TGGGGAAACAAGCTGATGAA+GGG | 0.608733 | 6.3:+81312833 | None:intergenic |
| GCAACAGTACTACATCTGGT+GGG | 0.617876 | 6.3:-81313218 | MS.gene72253:CDS |
| TATTGGGCACCTGCCCTAGG+AGG | 0.619502 | 6.3:-81309838 | MS.gene72253:CDS |
| AATAGCTATGCATATGGACA+TGG | 0.627229 | 6.3:-81317163 | MS.gene72253:CDS |
| ACCTCAGAAGATCGATCTAG+TGG | 0.628411 | 6.3:+81317046 | None:intergenic |
| CAACAGTACTACATCTGGTG+GGG | 0.629134 | 6.3:-81313217 | MS.gene72253:CDS |
| CTACAAGTTGGGAATGACAA+TGG | 0.634609 | 6.3:-81309868 | MS.gene72253:CDS |
| ACCACTAGATCGATCTTCTG+AGG | 0.634967 | 6.3:-81317047 | MS.gene72253:intron |
| TCAGAAGATCGATCTAGTGG+TGG | 0.660060 | 6.3:+81317049 | None:intergenic |
| TCCAAAGGATTACCTGACCA+GGG | 0.669498 | 6.3:+81310486 | None:intergenic |
| ACAATAGTAATATAGATGTG+AGG | 0.676915 | 6.3:-81317075 | MS.gene72253:CDS |
| AATAGCTTCTAACAAGACAG+AGG | 0.708748 | 6.3:+81312019 | None:intergenic |
| ACAATGGATCAGGAAACACA+AGG | 0.752248 | 6.3:-81311795 | MS.gene72253:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 81309822 | 81317300 | 81309822 | ID=MS.gene72253 |
| chr6.3 | mRNA | 81309822 | 81317300 | 81309822 | ID=MS.gene72253.t1;Parent=MS.gene72253 |
| chr6.3 | exon | 81317048 | 81317300 | 81317048 | ID=MS.gene72253.t1.exon1;Parent=MS.gene72253.t1 |
| chr6.3 | CDS | 81317048 | 81317300 | 81317048 | ID=cds.MS.gene72253.t1;Parent=MS.gene72253.t1 |
| chr6.3 | exon | 81313167 | 81313241 | 81313167 | ID=MS.gene72253.t1.exon2;Parent=MS.gene72253.t1 |
| chr6.3 | CDS | 81313167 | 81313241 | 81313167 | ID=cds.MS.gene72253.t1;Parent=MS.gene72253.t1 |
| chr6.3 | exon | 81312824 | 81312889 | 81312824 | ID=MS.gene72253.t1.exon3;Parent=MS.gene72253.t1 |
| chr6.3 | CDS | 81312824 | 81312889 | 81312824 | ID=cds.MS.gene72253.t1;Parent=MS.gene72253.t1 |
| chr6.3 | exon | 81311986 | 81312051 | 81311986 | ID=MS.gene72253.t1.exon4;Parent=MS.gene72253.t1 |
| chr6.3 | CDS | 81311986 | 81312051 | 81311986 | ID=cds.MS.gene72253.t1;Parent=MS.gene72253.t1 |
| chr6.3 | exon | 81311783 | 81311839 | 81311783 | ID=MS.gene72253.t1.exon5;Parent=MS.gene72253.t1 |
| chr6.3 | CDS | 81311783 | 81311839 | 81311783 | ID=cds.MS.gene72253.t1;Parent=MS.gene72253.t1 |
| chr6.3 | exon | 81310499 | 81310543 | 81310499 | ID=MS.gene72253.t1.exon6;Parent=MS.gene72253.t1 |
| chr6.3 | CDS | 81310499 | 81310543 | 81310499 | ID=cds.MS.gene72253.t1;Parent=MS.gene72253.t1 |
| chr6.3 | exon | 81310243 | 81310284 | 81310243 | ID=MS.gene72253.t1.exon7;Parent=MS.gene72253.t1 |
| chr6.3 | CDS | 81310243 | 81310284 | 81310243 | ID=cds.MS.gene72253.t1;Parent=MS.gene72253.t1 |
| chr6.3 | exon | 81309822 | 81309959 | 81309822 | ID=MS.gene72253.t1.exon8;Parent=MS.gene72253.t1 |
| chr6.3 | CDS | 81309822 | 81309959 | 81309822 | ID=cds.MS.gene72253.t1;Parent=MS.gene72253.t1 |
| Gene Sequence |
| Protein sequence |
