AlfalfaGEDB | MS.gene054433

M. sativa cultivar XinJiangDaYe / MS.gene054433

KEGG: -
GO: GO:0003674 GO:0003824
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054433.t1	XP_024625624.1	100	77	0	0	1	77	243	319	1.30E-36	161.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054433.t1	A0A072U5U0	100.0	77	0	0	1	77	243	319	9.3e-37	161.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050442	MS.gene054433	0.818943	1.51E-52	-1.69E-46
MS.gene053008	MS.gene054433	0.803531	3.33E-49	-1.69E-46
MS.gene054433	MS.gene054444	0.808479	3.03E-50	-1.69E-46
MS.gene054433	MS.gene054559	0.826746	2.29E-54	-1.69E-46
MS.gene054433	MS.gene055356	0.826748	2.29E-54	-1.69E-46
MS.gene054433	MS.gene055357	0.81039	1.18E-50	-1.69E-46
MS.gene054433	MS.gene055358	0.853013	3.16E-61	-1.69E-46
MS.gene054433	MS.gene055818	0.801202	1.01E-48	-1.69E-46
MS.gene054433	MS.gene057060	0.811024	8.61E-51	-1.69E-46
MS.gene054433	MS.gene057395	0.80724	5.57E-50	-1.69E-46
MS.gene054433	MS.gene059609	0.818316	2.09E-52	-1.69E-46
MS.gene054433	MS.gene060322	0.823081	1.68E-53	-1.69E-46
MS.gene054433	MS.gene060803	0.852942	3.31E-61	-1.69E-46
MS.gene054433	MS.gene060994	0.802565	5.28E-49	-1.69E-46
MS.gene054433	MS.gene061000	0.802652	5.07E-49	-1.69E-46
MS.gene054433	MS.gene061114	-0.807391	5.17E-50	-1.69E-46
MS.gene054433	MS.gene061333	0.834677	2.61E-56	-1.69E-46
MS.gene054433	MS.gene061334	0.844291	8.28E-59	-1.69E-46
MS.gene054433	MS.gene064944	0.823074	1.68E-53	-1.69E-46
MS.gene054433	MS.gene066950	0.813484	2.50E-51	-1.69E-46
MS.gene054433	MS.gene068609	0.835157	1.98E-56	-1.69E-46
MS.gene054433	MS.gene070553	0.804027	2.63E-49	-1.69E-46
MS.gene054433	MS.gene070695	0.804649	1.95E-49	-1.69E-46
MS.gene054433	MS.gene07115	0.840633	7.73E-58	-1.69E-46
MS.gene054433	MS.gene071693	-0.838053	3.61E-57	-1.69E-46
MS.gene054433	MS.gene072397	-0.800488	1.41E-48	-1.69E-46
MS.gene054433	MS.gene07344	0.809933	1.48E-50	-1.69E-46
MS.gene054433	MS.gene073460	0.806763	7.02E-50	-1.69E-46
MS.gene054433	MS.gene08324	-0.830022	3.71E-55	-1.69E-46
MS.gene054433	MS.gene08594	0.834258	3.33E-56	-1.69E-46
MS.gene054433	MS.gene08701	0.803552	3.30E-49	-1.69E-46
MS.gene054433	MS.gene24651	0.805436	1.34E-49	-1.69E-46
MS.gene054433	MS.gene24741	0.824636	7.25E-54	-1.69E-46
MS.gene054433	MS.gene25868	0.816609	5.06E-52	-1.69E-46
MS.gene054433	MS.gene28658	0.800512	1.39E-48	-1.69E-46
MS.gene054433	MS.gene29773	0.804946	1.69E-49	-1.69E-46
MS.gene054433	MS.gene32899	0.823726	1.19E-53	-1.69E-46
MS.gene054433	MS.gene33527	0.80239	5.74E-49	-1.69E-46
MS.gene054433	MS.gene34936	-0.820489	6.68E-53	-1.69E-46
MS.gene054433	MS.gene35408	0.806154	9.44E-50	-1.69E-46
MS.gene054433	MS.gene38876	0.800869	1.18E-48	-1.69E-46
MS.gene054433	MS.gene40837	-0.828656	7.96E-55	-1.69E-46
MS.gene054433	MS.gene45090	0.843042	1.79E-58	-1.69E-46
MS.gene054433	MS.gene45193	0.807375	5.21E-50	-1.69E-46
MS.gene054433	MS.gene48577	0.845746	3.35E-59	-1.69E-46
MS.gene054433	MS.gene51459	0.812217	4.74E-51	-1.69E-46
MS.gene054433	MS.gene53586	0.830196	3.37E-55	-1.69E-46
MS.gene054433	MS.gene55625	0.814856	1.25E-51	-1.69E-46
MS.gene054433	MS.gene57469	0.807578	4.72E-50	-1.69E-46
MS.gene054433	MS.gene58375	0.809055	2.29E-50	-1.69E-46
MS.gene054433	MS.gene59355	0.805745	1.15E-49	-1.69E-46
MS.gene054433	MS.gene59949	0.844182	8.86E-59	-1.69E-46
MS.gene054433	MS.gene60206	0.821018	5.05E-53	-1.69E-46
MS.gene054433	MS.gene61139	0.813765	2.17E-51	-1.69E-46
MS.gene054433	MS.gene61547	-0.808271	3.36E-50	-1.69E-46
MS.gene054433	MS.gene62393	0.863354	2.64E-64	-1.69E-46
MS.gene054433	MS.gene64249	0.827953	1.18E-54	-1.69E-46
MS.gene054433	MS.gene65718	0.801538	8.59E-49	-1.69E-46
MS.gene054433	MS.gene66964	0.803356	3.62E-49	-1.69E-46
MS.gene054433	MS.gene66969	0.801381	9.25E-49	-1.69E-46
MS.gene054433	MS.gene68923	0.816835	4.51E-52	-1.69E-46
MS.gene054433	MS.gene70189	0.804846	1.78E-49	-1.69E-46
MS.gene054433	MS.gene72253	0.800724	1.26E-48	-1.69E-46
MS.gene054433	MS.gene72268	0.824872	6.38E-54	-1.69E-46
MS.gene054433	MS.gene74971	0.805372	1.38E-49	-1.69E-46
MS.gene054433	MS.gene75304	0.8007	1.27E-48	-1.69E-46
MS.gene054433	MS.gene75490	0.846049	2.77E-59	-1.69E-46
MS.gene054433	MS.gene76773	-0.804782	1.83E-49	-1.69E-46
MS.gene054433	MS.gene77781	0.80359	3.24E-49	-1.69E-46
MS.gene054433	MS.gene87987	0.804998	1.65E-49	-1.69E-46
MS.gene054433	MS.gene91807	0.851729	7.34E-61	-1.69E-46
MS.gene054433	MS.gene91809	0.828974	6.67E-55	-1.69E-46
MS.gene054433	MS.gene95377	0.805561	1.26E-49	-1.69E-46
MS.gene054433	MS.gene98445	-0.807464	4.99E-50	-1.69E-46
MS.gene054433	MS.gene98543	0.813755	2.18E-51	-1.69E-46
MS.gene054433	MS.gene99735	0.803443	3.48E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054433.t1	MTR_7g112615	100.000	77	0	0	1	77	243	319	2.16e-49	157
MS.gene054433.t1	MTR_7g113020	96.053	76	3	0	2	77	244	319	1.63e-46	150
MS.gene054433.t1	MTR_7g113030	93.506	77	5	0	1	77	243	319	1.93e-46	150
MS.gene054433.t1	MTR_7g113050	93.506	77	5	0	1	77	243	319	2.47e-46	150
MS.gene054433.t1	MTR_7g112620	86.842	76	10	0	2	77	244	319	3.00e-41	137
MS.gene054433.t1	MTR_7g112963	78.947	76	16	0	2	77	244	319	8.66e-37	125
MS.gene054433.t1	MTR_7g112970	75.000	76	19	0	2	77	241	316	1.26e-36	125
MS.gene054433.t1	MTR_7g112960	77.632	76	17	0	2	77	244	319	1.95e-36	124
MS.gene054433.t1	MTR_7g108825	68.831	77	24	0	1	77	241	317	2.76e-34	119
MS.gene054433.t1	MTR_4g005160	67.532	77	24	1	2	77	246	322	9.42e-32	112
MS.gene054433.t1	MTR_7g112910	69.737	76	10	1	2	77	133	195	7.53e-28	99.4
MS.gene054433.t1	MTR_4g120240	57.895	76	32	0	2	77	280	355	6.58e-27	100
MS.gene054433.t1	MTR_8g076680	44.156	77	41	1	1	77	243	317	1.50e-13	63.5
MS.gene054433.t1	MTR_7g113010	71.053	38	11	0	2	39	244	281	2.12e-12	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054433.t1	AT4G02340	61.842	76	29	0	2	77	241	316	2.60e-29	106
MS.gene054433.t1	AT3G05600	57.895	76	32	0	2	77	249	324	1.36e-27	101
MS.gene054433.t1	AT2G26740	61.039	77	30	0	1	77	245	321	1.02e-25	96.7
MS.gene054433.t1	AT2G26750	58.442	77	32	0	1	77	244	320	1.26e-24	93.6
MS.gene054433.t1	AT4G15960	56.579	76	33	0	2	77	299	374	1.54e-24	94.4
MS.gene054433.t1	AT4G15955	50.649	77	38	0	1	77	228	304	5.19e-20	81.3
MS.gene054433.t1	AT3G51000	47.436	78	39	2	1	77	243	319	3.14e-14	65.9

Find 20 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAAGGAAGATGTGCCGAATT+TGG	0.238850	7.4:-4120874	MS.gene054433:CDS
GAAGAAGTGATTGTCCAAAA+AGG	0.365676	7.4:-4120852	MS.gene054433:CDS
ACAATCACTTCTTCCAAATT+CGG	0.378669	7.4:+4120861	None:intergenic
TGGATATACTCCTTCACCTT+TGG	0.407549	7.4:+4120909	None:intergenic
GTGCCTGTGAAATTCATTAC+AGG	0.432209	7.4:-4120957	MS.gene054433:CDS
TATCCATGGTGGAGGGTTCA+AGG	0.477350	7.4:-4120892	MS.gene054433:CDS
GAAATTCATTACAGGTGACT+TGG	0.543660	7.4:-4120949	MS.gene054433:CDS
AAGGAGTATATCCATGGTGG+AGG	0.549978	7.4:-4120900	MS.gene054433:CDS
GAGCTGATGGCACCATGGAA+TGG	0.569405	7.4:-4120993	None:intergenic
ATACTCCTTCACCTTTGGTG+AGG	0.584740	7.4:+4120914	None:intergenic
TCACCTGTAATGAATTTCAC+AGG	0.591992	7.4:+4120954	None:intergenic
ATGGAATGGAGTGAAAATCA+AGG	0.596236	7.4:-4120979	MS.gene054433:CDS
CAACAATCAAGAAGCAGCAG+AGG	0.610084	7.4:-4120817	MS.gene054433:CDS
AAGGTGAAGGAGTATATCCA+TGG	0.634923	7.4:-4120906	MS.gene054433:CDS
ATACACCTCACCAAAGGTGA+AGG	0.642917	7.4:-4120919	MS.gene054433:CDS
CATTGTATACACCTCACCAA+AGG	0.655794	7.4:-4120925	MS.gene054433:CDS
CTTCCTTGAACCCTCCACCA+TGG	0.658760	7.4:+4120889	None:intergenic
ATTGGGAGCTGATGGCACCA+TGG	0.668822	7.4:-4120998	None:intergenic
AGGAGTATATCCATGGTGGA+GGG	0.690032	7.4:-4120899	MS.gene054433:CDS
GTGAAGGAGTATATCCATGG+TGG	0.691244	7.4:-4120903	MS.gene054433:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAATCACTTCTTCCAAATT+CGG	+	chr7.4:4120905-4120924	None:intergenic	30.0%
	ATGGAATGGAGTGAAAATCA+AGG	-	chr7.4:4120784-4120803	MS.gene054433:CDS	35.0%
	GAAATTCATTACAGGTGACT+TGG	-	chr7.4:4120814-4120833	MS.gene054433:CDS	35.0%
	GAAGAAGTGATTGTCCAAAA+AGG	-	chr7.4:4120911-4120930	MS.gene054433:CDS	35.0%
	TCACCTGTAATGAATTTCAC+AGG	+	chr7.4:4120812-4120831	None:intergenic	35.0%
!	TTGATTTTCACTCCATTCCA+TGG	+	chr7.4:4120785-4120804	None:intergenic	35.0%
	AGTGATTGTCCAAAAAGGAG+TGG	-	chr7.4:4120916-4120935	MS.gene054433:CDS	40.0%
	CATTGTATACACCTCACCAA+AGG	-	chr7.4:4120838-4120857	MS.gene054433:CDS	40.0%
	GTGCCTGTGAAATTCATTAC+AGG	-	chr7.4:4120806-4120825	MS.gene054433:CDS	40.0%
	TGGATATACTCCTTCACCTT+TGG	+	chr7.4:4120857-4120876	None:intergenic	40.0%
!	AAGGTGAAGGAGTATATCCA+TGG	-	chr7.4:4120857-4120876	MS.gene054433:CDS	40.0%
	ATACACCTCACCAAAGGTGA+AGG	-	chr7.4:4120844-4120863	MS.gene054433:CDS	45.0%
	ATACTCCTTCACCTTTGGTG+AGG	+	chr7.4:4120852-4120871	None:intergenic	45.0%
	CAACAATCAAGAAGCAGCAG+AGG	-	chr7.4:4120946-4120965	MS.gene054433:CDS	45.0%
	CAAGGAAGATGTGCCGAATT+TGG	-	chr7.4:4120889-4120908	MS.gene054433:CDS	45.0%
!	AAGGAGTATATCCATGGTGG+AGG	-	chr7.4:4120863-4120882	MS.gene054433:CDS	45.0%
!	AGGAGTATATCCATGGTGGA+GGG	-	chr7.4:4120864-4120883	MS.gene054433:CDS	45.0%
!	GTGAAGGAGTATATCCATGG+TGG	-	chr7.4:4120860-4120879	MS.gene054433:CDS	45.0%
!	TGAAGTGAGCCACTCCTTTT+TGG	+	chr7.4:4120928-4120947	None:intergenic	45.0%
!	TATCCATGGTGGAGGGTTCA+AGG	-	chr7.4:4120871-4120890	MS.gene054433:CDS	50.0%
	CTTCCTTGAACCCTCCACCA+TGG	+	chr7.4:4120877-4120896	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	4120776	4121009	4120776	ID=MS.gene054433
chr7.4	mRNA	4120776	4121009	4120776	ID=MS.gene054433.t1;Parent=MS.gene054433
chr7.4	exon	4120776	4121009	4120776	ID=MS.gene054433.t1.exon1;Parent=MS.gene054433.t1
chr7.4	CDS	4120776	4121009	4120776	ID=cds.MS.gene054433.t1;Parent=MS.gene054433.t1

Gene Sequence	>MS.gene054433 ATGGCACCATGGAATGGAGTGAAAATCAAGGTGCCTGTGAAATTCATTACAGGTGACTTGGACATTGTATACACCTCACCAAAGGTGAAGGAGTATATCCATGGTGGAGGGTTCAAGGAAGATGTGCCGAATTTGGAAGAAGTGATTGTCCAAAAAGGAGTGGCTCACTTCAACAATCAAGAAGCAGCAGAGGAAATCAGTAATCACATTTATGAATTCATCAAGAAGTTCTGA
Protein sequence	>MS.gene054433.t1 MAPWNGVKIKVPVKFITGDLDIVYTSPKVKEYIHGGGFKEDVPNLEEVIVQKGVAHFNNQEAAEEISNHIYEFIKKF