Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72315.t1 | XP_013468028.1 | 96.3 | 54 | 2 | 0 | 1 | 54 | 1 | 54 | 7.30E-18 | 99.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72315.t1 | P41099 | 63.0 | 54 | 20 | 0 | 1 | 54 | 1 | 54 | 1.3e-12 | 73.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72315.t1 | I3S066 | 96.3 | 54 | 2 | 0 | 1 | 54 | 1 | 54 | 5.2e-18 | 99.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051676 | MS.gene72315 | 0.803427 | 3.50E-49 | -1.69E-46 |
| MS.gene051700 | MS.gene72315 | 0.814309 | 1.65E-51 | -1.69E-46 |
| MS.gene051744 | MS.gene72315 | 0.808388 | 3.17E-50 | -1.69E-46 |
| MS.gene051975 | MS.gene72315 | 0.82404 | 1.00E-53 | -1.69E-46 |
| MS.gene052947 | MS.gene72315 | 0.810351 | 1.20E-50 | -1.69E-46 |
| MS.gene056215 | MS.gene72315 | 0.801606 | 8.32E-49 | -1.69E-46 |
| MS.gene05689 | MS.gene72315 | 0.824092 | 9.73E-54 | -1.69E-46 |
| MS.gene057694 | MS.gene72315 | 0.809021 | 2.33E-50 | -1.69E-46 |
| MS.gene057705 | MS.gene72315 | 0.810047 | 1.40E-50 | -1.69E-46 |
| MS.gene059227 | MS.gene72315 | 0.820036 | 8.48E-53 | -1.69E-46 |
| MS.gene05985 | MS.gene72315 | -0.809483 | 1.85E-50 | -1.69E-46 |
| MS.gene06001 | MS.gene72315 | 0.818709 | 1.70E-52 | -1.69E-46 |
| MS.gene060365 | MS.gene72315 | 0.809141 | 2.19E-50 | -1.69E-46 |
| MS.gene060429 | MS.gene72315 | 0.818109 | 2.33E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72315.t1 | MTR_1g060600 | 96.296 | 54 | 2 | 0 | 1 | 54 | 1 | 54 | 1.85e-30 | 103 |
| MS.gene72315.t1 | MTR_2g085085 | 68.519 | 54 | 17 | 0 | 1 | 54 | 1 | 54 | 3.25e-20 | 77.4 |
| MS.gene72315.t1 | MTR_0227s0020 | 66.667 | 54 | 18 | 0 | 1 | 54 | 1 | 54 | 3.99e-19 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72315.t1 | AT2G27710 | 66.667 | 54 | 18 | 0 | 1 | 54 | 1 | 54 | 6.04e-19 | 73.9 |
| MS.gene72315.t1 | AT2G27710 | 66.667 | 54 | 18 | 0 | 1 | 54 | 1 | 54 | 7.26e-19 | 73.9 |
| MS.gene72315.t1 | AT2G27710 | 66.667 | 54 | 18 | 0 | 1 | 54 | 1 | 54 | 7.26e-19 | 73.9 |
| MS.gene72315.t1 | AT2G27710 | 66.667 | 54 | 18 | 0 | 1 | 54 | 1 | 54 | 7.26e-19 | 73.9 |
| MS.gene72315.t1 | AT2G27720 | 66.667 | 54 | 18 | 0 | 1 | 54 | 1 | 54 | 1.15e-18 | 73.6 |
| MS.gene72315.t1 | AT3G44590 | 64.815 | 54 | 19 | 0 | 1 | 54 | 1 | 54 | 6.07e-18 | 71.6 |
| MS.gene72315.t1 | AT3G44590 | 64.815 | 54 | 19 | 0 | 1 | 54 | 1 | 54 | 6.07e-18 | 71.6 |
| MS.gene72315.t1 | AT2G27720 | 54.545 | 66 | 18 | 1 | 1 | 54 | 1 | 66 | 5.31e-15 | 64.7 |
| MS.gene72315.t1 | AT2G27720 | 50.725 | 69 | 19 | 1 | 1 | 54 | 1 | 69 | 1.04e-13 | 61.2 |
| MS.gene72315.t1 | AT5G40040 | 51.852 | 54 | 26 | 0 | 1 | 54 | 1 | 54 | 6.64e-12 | 56.2 |
| MS.gene72315.t1 | AT3G28500 | 48.148 | 54 | 28 | 0 | 1 | 54 | 1 | 54 | 3.87e-11 | 54.3 |
Find 15 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTATTTGCTCGCTGTTCT+TGG | 0.258518 | 1.4:+49678181 | MS.gene72315:CDS |
| GAGGATGTCCTTGATGGTTT+TGG | 0.299226 | 1.4:-49678224 | None:intergenic |
| ATCAAGGACATCCTCGGATC+TGG | 0.454124 | 1.4:+49678232 | MS.gene72315:CDS |
| CAGCGGCTGCGATTGACTAC+AGG | 0.464733 | 1.4:-49679163 | None:intergenic |
| ACCAAGAACAGCGAGCAAAT+AGG | 0.468861 | 1.4:-49678182 | None:intergenic |
| AGATCCGAGGATGTCCTTGA+TGG | 0.481716 | 1.4:-49678230 | None:intergenic |
| GAGCAGACGCAGAAGATGAT+AGG | 0.489365 | 1.4:+49678756 | MS.gene72315:CDS |
| TATTTGCTCGCTGTTCTTGG+TGG | 0.547005 | 1.4:+49678184 | MS.gene72315:CDS |
| AGCGGCTGCGATTGACTACA+GGG | 0.556515 | 1.4:-49679162 | None:intergenic |
| CCCCTCCGCCAAAACCATCA+AGG | 0.565900 | 1.4:+49678216 | MS.gene72315:CDS |
| AAAACCATCAAGGACATCCT+CGG | 0.583067 | 1.4:+49678226 | MS.gene72315:CDS |
| ATTATGCAACACTGCTACAG+CGG | 0.602909 | 1.4:-49679180 | None:intergenic |
| GAAGAAACGAACCAGATCCG+AGG | 0.673984 | 1.4:-49678243 | None:intergenic |
| TAAGGGCAAAGATATAGCTG+AGG | 0.689474 | 1.4:+49678802 | MS.gene72315:CDS |
| GCGGCTGCGATTGACTACAG+GGG | 0.763228 | 1.4:-49679161 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTTAGAACTGTTTTTA+AGG | + | chr1.4:49678497-49678516 | MS.gene72315:intron | 15.0% |
| !!! | TCATTTTCTTAAATAATCAT+CGG | - | chr1.4:49678542-49678561 | None:intergenic | 15.0% |
| !!! | TGATTTCGATTTTTTTTTAT+CGG | + | chr1.4:49678435-49678454 | MS.gene72315:intron | 15.0% |
| !!! | TTGTTTTTGTCTGAAATTAA+GGG | + | chr1.4:49678785-49678804 | MS.gene72315:CDS | 20.0% |
| ! | CACATAATGCAAATTAAACT+AGG | - | chr1.4:49679090-49679109 | None:intergenic | 25.0% |
| !! | ATGATGTAAGTTCTTTTTCT+GGG | + | chr1.4:49678972-49678991 | MS.gene72315:intron | 25.0% |
| !! | TATGTGTTTTATACTCGAAA+AGG | + | chr1.4:49679104-49679123 | MS.gene72315:intron | 25.0% |
| !!! | GTTGTTTTTGTCTGAAATTA+AGG | + | chr1.4:49678784-49678803 | MS.gene72315:CDS | 25.0% |
| ATCACATGTGTTAGTTGTAT+CGG | + | chr1.4:49678576-49678595 | MS.gene72315:intron | 30.0% | |
| TTATATGTATGCGCTATAAG+TGG | + | chr1.4:49679038-49679057 | MS.gene72315:intron | 30.0% | |
| TTATGCAGTAGCGTAAATTA+GGG | + | chr1.4:49678407-49678426 | MS.gene72315:intron | 30.0% | |
| TTTATGCAGTAGCGTAAATT+AGG | + | chr1.4:49678406-49678425 | MS.gene72315:intron | 30.0% | |
| ! | ACACATTCAATTAGAAGTGT+CGG | + | chr1.4:49678631-49678650 | MS.gene72315:intron | 30.0% |
| ! | GATGATGTAAGTTCTTTTTC+TGG | + | chr1.4:49678971-49678990 | MS.gene72315:intron | 30.0% |
| ! | GATTTGAAACATAACTGACA+GGG | + | chr1.4:49678709-49678728 | MS.gene72315:intron | 30.0% |
| ! | TGATTTGAAACATAACTGAC+AGG | + | chr1.4:49678708-49678727 | MS.gene72315:intron | 30.0% |
| !! | AAGGTTCTTGATTTGTACTT+AGG | + | chr1.4:49679123-49679142 | MS.gene72315:intron | 30.0% |
| !! | GTTTTAAACTTGCATTTGGT+TGG | + | chr1.4:49679060-49679079 | MS.gene72315:intron | 30.0% |
| !! | TTTGTTGTTTGATTTGCAGT+TGG | + | chr1.4:49678734-49678753 | MS.gene72315:intron | 30.0% |
| !!! | AGTGGTTTTAAACTTGCATT+TGG | + | chr1.4:49679056-49679075 | MS.gene72315:intron | 30.0% |
| CTTCTAATTGAATGTGTGTC+CGG | - | chr1.4:49678628-49678647 | None:intergenic | 35.0% | |
| AAAACCATCAAGGACATCCT+CGG | + | chr1.4:49678226-49678245 | MS.gene72315:CDS | 40.0% | |
| AAATTGGCATCAGTTCCTTC+TGG | + | chr1.4:49678845-49678864 | MS.gene72315:intron | 40.0% | |
| ATAGCTGAGGTTATTGCTTC+TGG | + | chr1.4:49678815-49678834 | MS.gene72315:intron | 40.0% | |
| ATTTGACATGACACCTTGAG+GGG | + | chr1.4:49678674-49678693 | MS.gene72315:intron | 40.0% | |
| GATTTGACATGACACCTTGA+GGG | + | chr1.4:49678673-49678692 | MS.gene72315:intron | 40.0% | |
| TAAGGGCAAAGATATAGCTG+AGG | + | chr1.4:49678802-49678821 | MS.gene72315:CDS | 40.0% | |
| TATTCGACATTGACCTGTGT+TGG | + | chr1.4:49678599-49678618 | MS.gene72315:intron | 40.0% | |
| TGAGGTTATTGCTTCTGGTA+GGG | + | chr1.4:49678820-49678839 | MS.gene72315:intron | 40.0% | |
| TGATTTGACATGACACCTTG+AGG | + | chr1.4:49678672-49678691 | MS.gene72315:intron | 40.0% | |
| TGCTTCTGGTAGGGAAAAAT+TGG | + | chr1.4:49678829-49678848 | MS.gene72315:intron | 40.0% | |
| ! | AAGGTGTCATGTCAAATCAG+TGG | - | chr1.4:49678671-49678690 | None:intergenic | 40.0% |
| ACCAAGAACAGCGAGCAAAT+AGG | - | chr1.4:49678185-49678204 | None:intergenic | 45.0% | |
| CTGAGGTTATTGCTTCTGGT+AGG | + | chr1.4:49678819-49678838 | MS.gene72315:intron | 45.0% | |
| TATTTGCTCGCTGTTCTTGG+TGG | + | chr1.4:49678184-49678203 | MS.gene72315:CDS | 45.0% | |
| TGAGGCAAAGAAAGAGGAGA+AGG | + | chr1.4:49678928-49678947 | MS.gene72315:intron | 45.0% | |
| !! | GAGGATGTCCTTGATGGTTT+TGG | - | chr1.4:49678227-49678246 | None:intergenic | 45.0% |
| !!! | GATGTCCTTGATGGTTTTGG+CGG | - | chr1.4:49678224-49678243 | None:intergenic | 45.0% |
| !!! | ATTTTAATAATTTGATAAAT+CGG | + | chr1.4:49678367-49678386 | MS.gene72315:intron | 5.0% |
| AGATCCGAGGATGTCCTTGA+TGG | - | chr1.4:49678233-49678252 | None:intergenic | 50.0% | |
| AGCTGCTGAGGCAAAGAAAG+AGG | + | chr1.4:49678922-49678941 | MS.gene72315:intron | 50.0% | |
| ATCAAGGACATCCTCGGATC+TGG | + | chr1.4:49678232-49678251 | MS.gene72315:CDS | 50.0% | |
| CATTGACCTGTGTTGGACAC+CGG | + | chr1.4:49678606-49678625 | MS.gene72315:intron | 50.0% | |
| GAAGAAACGAACCAGATCCG+AGG | - | chr1.4:49678246-49678265 | None:intergenic | 50.0% | |
| GAGCAGACGCAGAAGATGAT+AGG | + | chr1.4:49678756-49678775 | MS.gene72315:CDS | 50.0% | |
| GCCTATTTGCTCGCTGTTCT+TGG | + | chr1.4:49678181-49678200 | MS.gene72315:CDS | 50.0% | |
| GGCAAAGAAAGAGGAGAAGG+TGG | + | chr1.4:49678931-49678950 | MS.gene72315:intron | 50.0% | |
| TTGGCATCAGTTCCTTCTGG+TGG | + | chr1.4:49678848-49678867 | MS.gene72315:intron | 50.0% | |
| !!! | TCCTTGATGGTTTTGGCGGA+GGG | - | chr1.4:49678220-49678239 | None:intergenic | 50.0% |
| AGCGGCTGCGATTGACTACA+GGG | - | chr1.4:49679165-49679184 | None:intergenic | 55.0% | |
| CAGCGAGTTCAAACCCCTCA+AGG | - | chr1.4:49678690-49678709 | None:intergenic | 55.0% | |
| GCATCAGTTCCTTCTGGTGG+TGG | + | chr1.4:49678851-49678870 | MS.gene72315:intron | 55.0% | |
| TTCTTTGCCTCAGCAGCTGC+AGG | - | chr1.4:49678920-49678939 | None:intergenic | 55.0% | |
| !! | TCAGTTCCTTCTGGTGGTGG+CGG | + | chr1.4:49678854-49678873 | MS.gene72315:intron | 55.0% |
| !!! | CCTTGATGGTTTTGGCGGAG+GGG | - | chr1.4:49678219-49678238 | None:intergenic | 55.0% |
| !!! | CTTGATGGTTTTGGCGGAGG+GGG | - | chr1.4:49678218-49678237 | None:intergenic | 55.0% |
| !!! | GTCCTTGATGGTTTTGGCGG+AGG | - | chr1.4:49678221-49678240 | None:intergenic | 55.0% |
| CAGCGGCTGCGATTGACTAC+AGG | - | chr1.4:49679166-49679185 | None:intergenic | 60.0% | |
| CCCCTCCGCCAAAACCATCA+AGG | + | chr1.4:49678216-49678235 | MS.gene72315:CDS | 60.0% | |
| GCGGCTGCGATTGACTACAG+GGG | - | chr1.4:49679164-49679183 | None:intergenic | 60.0% | |
| GTGTGTCCGGTGTCCAACAC+AGG | - | chr1.4:49678615-49678634 | None:intergenic | 60.0% | |
| !! | TGGCGGTGTTGCTGTTGCTG+CGG | + | chr1.4:49678871-49678890 | MS.gene72315:intron | 60.0% |
| GCAACACCGCCACCACCAGA+AGG | - | chr1.4:49678863-49678882 | None:intergenic | 65.0% | |
| TGCTGCTCCTGCAGCTGCTG+AGG | + | chr1.4:49678910-49678929 | MS.gene72315:intron | 65.0% | |
| GGAGCAGCACCACCACTCGC+AGG | - | chr1.4:49678899-49678918 | None:intergenic | 70.0% | |
| GTTGCTGCGGCACCTGCGAG+TGG | + | chr1.4:49678884-49678903 | MS.gene72315:intron | 70.0% | |
| GCTGCGGCACCTGCGAGTGG+TGG | + | chr1.4:49678887-49678906 | MS.gene72315:intron | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 49678166 | 49679201 | 49678166 | ID=MS.gene72315 |
| chr1.4 | mRNA | 49678166 | 49679201 | 49678166 | ID=MS.gene72315.t1;Parent=MS.gene72315 |
| chr1.4 | exon | 49678166 | 49678253 | 49678166 | ID=MS.gene72315.t1.exon1;Parent=MS.gene72315.t1 |
| chr1.4 | CDS | 49678166 | 49678253 | 49678166 | ID=cds.MS.gene72315.t1;Parent=MS.gene72315.t1 |
| chr1.4 | exon | 49678753 | 49678823 | 49678753 | ID=MS.gene72315.t1.exon2;Parent=MS.gene72315.t1 |
| chr1.4 | CDS | 49678753 | 49678823 | 49678753 | ID=cds.MS.gene72315.t1;Parent=MS.gene72315.t1 |
| chr1.4 | exon | 49679145 | 49679201 | 49679145 | ID=MS.gene72315.t1.exon3;Parent=MS.gene72315.t1 |
| chr1.4 | CDS | 49679145 | 49679201 | 49679145 | ID=cds.MS.gene72315.t1;Parent=MS.gene72315.t1 |
| Gene Sequence |
| Protein sequence |
