Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73226.t1 | XP_013454941.1 | 89.5 | 76 | 1 | 1 | 1 | 69 | 1 | 76 | 1.50E-29 | 138.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73226.t1 | A0A072UGV8 | 89.5 | 76 | 1 | 1 | 1 | 69 | 1 | 76 | 1.1e-29 | 138.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049297 | MS.gene73226 | 0.811528 | 6.69E-51 | -1.69E-46 |
| MS.gene050226 | MS.gene73226 | 0.839921 | 1.19E-57 | -1.69E-46 |
| MS.gene050227 | MS.gene73226 | 0.83646 | 9.24E-57 | -1.69E-46 |
| MS.gene050228 | MS.gene73226 | 0.840659 | 7.61E-58 | -1.69E-46 |
| MS.gene050301 | MS.gene73226 | 0.801969 | 7.01E-49 | -1.69E-46 |
| MS.gene050330 | MS.gene73226 | -0.819357 | 1.21E-52 | -1.69E-46 |
| MS.gene050558 | MS.gene73226 | 0.84685 | 1.68E-59 | -1.69E-46 |
| MS.gene050820 | MS.gene73226 | 0.812706 | 3.70E-51 | -1.69E-46 |
| MS.gene051860 | MS.gene73226 | 0.810986 | 8.78E-51 | -1.69E-46 |
| MS.gene051983 | MS.gene73226 | 0.803308 | 3.71E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene73226 | 0.801631 | 8.22E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene73226 | 0.847484 | 1.12E-59 | -1.69E-46 |
| MS.gene054930 | MS.gene73226 | 0.843099 | 1.73E-58 | -1.69E-46 |
| MS.gene054983 | MS.gene73226 | 0.804944 | 1.69E-49 | -1.69E-46 |
| MS.gene056875 | MS.gene73226 | 0.805108 | 1.56E-49 | -1.69E-46 |
| MS.gene057121 | MS.gene73226 | 0.825214 | 5.29E-54 | -1.69E-46 |
| MS.gene058142 | MS.gene73226 | 0.805744 | 1.15E-49 | -1.69E-46 |
| MS.gene058736 | MS.gene73226 | 0.812602 | 3.90E-51 | -1.69E-46 |
| MS.gene059128 | MS.gene73226 | 0.803545 | 3.31E-49 | -1.69E-46 |
| MS.gene061085 | MS.gene73226 | 0.80793 | 3.97E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73226.t1 | MTR_4g019910 | 89.474 | 76 | 1 | 1 | 1 | 69 | 1 | 76 | 1.86e-44 | 143 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTAACTTATTTGGATTAA+AGG | 0.134555 | 4.1:-78274858 | None:intergenic |
| TTGCCAAAAGCTAACTTATT+TGG | 0.143563 | 4.1:-78274866 | None:intergenic |
| AATCCAAATAAGTTAGCTTT+TGG | 0.283724 | 4.1:+78274863 | MS.gene73226:CDS |
| CAAGGAGTTCAGTTTCTTGC+CGG | 0.285437 | 4.1:+78274787 | MS.gene73226:intron |
| GTTCAGTTTCTTGCCGGGTT+CGG | 0.410478 | 4.1:+78274793 | MS.gene73226:CDS |
| TGCAACTTGAGGCATTGATT+AGG | 0.431017 | 4.1:-78274923 | None:intergenic |
| TCAGGCTATAAAATATGTTA+TGG | 0.433539 | 4.1:-78275342 | None:intergenic |
| ATCTTCTCATTTGCAACTTG+AGG | 0.480992 | 4.1:-78274934 | None:intergenic |
| AAGGAGTTCAGTTTCTTGCC+GGG | 0.510954 | 4.1:+78274788 | MS.gene73226:intron |
| AAGCTATTGATGACCGAACC+CGG | 0.564308 | 4.1:-78274806 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTATTAAATTAAGTGTA+GGG | + | chr4.1:78275318-78275337 | MS.gene73226:intron | 10.0% |
| !!! | TATTTTATTAAATTAAGTGT+AGG | + | chr4.1:78275136-78275155 | MS.gene73226:intron | 10.0% |
| !! | AACACTTATAAAATATGTTA+TGG | - | chr4.1:78275245-78275264 | None:intergenic | 15.0% |
| !!! | AATTCTCTTTTAAGTTTTAA+GGG | + | chr4.1:78274406-78274425 | MS.gene73226:intron | 15.0% |
| !! | TACATAGAATTTCAATTGAA+TGG | + | chr4.1:78274578-78274597 | MS.gene73226:intron | 20.0% |
| !!! | AATTTTGTGTTTTTTCATGA+AGG | + | chr4.1:78274748-78274767 | MS.gene73226:intron | 20.0% |
| !!! | ATTTTGTGTTTTTTCATGAA+GGG | + | chr4.1:78274749-78274768 | MS.gene73226:intron | 20.0% |
| !!! | GAATTCTCTTTTAAGTTTTA+AGG | + | chr4.1:78274405-78274424 | MS.gene73226:intron | 20.0% |
| !!! | TTTGATTTAACTACTTGAAA+TGG | + | chr4.1:78274510-78274529 | MS.gene73226:intron | 20.0% |
| ! | AACTACTTGAAATGGAAATT+TGG | + | chr4.1:78274518-78274537 | MS.gene73226:intron | 25.0% |
| ! | AGCTAACTTATTTGGATTAA+AGG | - | chr4.1:78274861-78274880 | None:intergenic | 25.0% |
| ! | AGTACCAATAAGAATCAAAA+GGG | - | chr4.1:78274983-78275002 | None:intergenic | 25.0% |
| ! | ATAGTTAGTACAGAAAACTT+AGG | - | chr4.1:78275119-78275138 | None:intergenic | 25.0% |
| ! | ATCAAGACTATAGAAAGAAA+TGG | - | chr4.1:78275062-78275081 | None:intergenic | 25.0% |
| ! | ATCTATCGAATTCAGAATTT+CGG | + | chr4.1:78274474-78274493 | MS.gene73226:intron | 25.0% |
| ! | CTTCAGTGAAATTCATAAAA+AGG | - | chr4.1:78274619-78274638 | None:intergenic | 25.0% |
| ! | TAGAATTTCAATTGAATGGT+TGG | + | chr4.1:78274582-78274601 | MS.gene73226:intron | 25.0% |
| ! | TAGTACCAATAAGAATCAAA+AGG | - | chr4.1:78274984-78275003 | None:intergenic | 25.0% |
| ! | TCAGGCTATAAAATATGTTA+TGG | - | chr4.1:78275345-78275364 | None:intergenic | 25.0% |
| !! | AATTTAAAAATTTGCTCACG+TGG | + | chr4.1:78274713-78274732 | MS.gene73226:intron | 25.0% |
| !!! | AATCCAAATAAGTTAGCTTT+TGG | + | chr4.1:78274863-78274882 | MS.gene73226:CDS | 25.0% |
| !!! | ATAAGTGTAATTCTGAAACA+AGG | - | chr4.1:78274366-78274385 | None:intergenic | 25.0% |
| !!! | CGATATGTTTTTCTTTTTCA+AGG | + | chr4.1:78274676-78274695 | MS.gene73226:intron | 25.0% |
| !!! | TAAGTGTAATTCTGAAACAA+GGG | - | chr4.1:78274365-78274384 | None:intergenic | 25.0% |
| !!! | TTCTTATTGGTACTACTTTT+TGG | + | chr4.1:78274989-78275008 | MS.gene73226:intron | 25.0% |
| AGCCTAGATGCAAATATAAT+AGG | - | chr4.1:78275096-78275115 | None:intergenic | 30.0% | |
| CTTGAAATGGAAATTTGGAA+TGG | + | chr4.1:78274523-78274542 | MS.gene73226:intron | 30.0% | |
| GACCTATTATATTTGCATCT+AGG | + | chr4.1:78275091-78275110 | MS.gene73226:intron | 30.0% | |
| GTAAAAAAAAATCCAACTCC+CGG | - | chr4.1:78274561-78274580 | None:intergenic | 30.0% | |
| TCTATCGAATTCAGAATTTC+GGG | + | chr4.1:78274475-78274494 | MS.gene73226:intron | 30.0% | |
| TTGCCAAAAGCTAACTTATT+TGG | - | chr4.1:78274869-78274888 | None:intergenic | 30.0% | |
| TTTCTGCAAAATTATCGCTT+AGG | + | chr4.1:78274429-78274448 | MS.gene73226:intron | 30.0% | |
| ! | TTATAAGTGTCGTCAAATAG+CGG | + | chr4.1:78275162-78275181 | MS.gene73226:intron | 30.0% |
| !!! | TTTGGCAAAAGTTTTGAAAG+AGG | + | chr4.1:78274881-78274900 | MS.gene73226:CDS | 30.0% |
| AAGAAGTCTCACTTCATGAA+TGG | + | chr4.1:78274636-78274655 | MS.gene73226:intron | 35.0% | |
| ATCTTCTCATTTGCAACTTG+AGG | - | chr4.1:78274937-78274956 | None:intergenic | 35.0% | |
| TCATGAATGGTTGAGGAATA+AGG | + | chr4.1:78274649-78274668 | MS.gene73226:intron | 35.0% | |
| !! | CGAACCCTTTTGATTCTTAT+TGG | + | chr4.1:78274976-78274995 | MS.gene73226:intron | 35.0% |
| AAAAAAAATCCAACTCCCGG+AGG | - | chr4.1:78274558-78274577 | None:intergenic | 40.0% | |
| AATCAAAAGGGTTCGAGCAA+AGG | - | chr4.1:78274971-78274990 | None:intergenic | 40.0% | |
| GAATGGTTTATAACTCCTCC+GGG | + | chr4.1:78274540-78274559 | MS.gene73226:intron | 40.0% | |
| GGAATGGTTTATAACTCCTC+CGG | + | chr4.1:78274539-78274558 | MS.gene73226:intron | 40.0% | |
| TCTCACTTCATGAATGGTTG+AGG | + | chr4.1:78274642-78274661 | MS.gene73226:intron | 40.0% | |
| ! | AAATTTGCTCACGTGGTTCA+TGG | + | chr4.1:78274720-78274739 | MS.gene73226:intron | 40.0% |
| !! | TGCAACTTGAGGCATTGATT+AGG | - | chr4.1:78274926-78274945 | None:intergenic | 40.0% |
| AAGGAGTTCAGTTTCTTGCC+GGG | + | chr4.1:78274788-78274807 | MS.gene73226:intron | 45.0% | |
| CAAGGAGTTCAGTTTCTTGC+CGG | + | chr4.1:78274787-78274806 | MS.gene73226:intron | 45.0% | |
| GGGACTTTCTTCTACTCTCA+AGG | + | chr4.1:78274769-78274788 | MS.gene73226:intron | 45.0% | |
| TTTATAACTCCTCCGGGAGT+TGG | + | chr4.1:78274546-78274565 | MS.gene73226:intron | 45.0% | |
| ! | AAGCTATTGATGACCGAACC+CGG | - | chr4.1:78274809-78274828 | None:intergenic | 45.0% |
| !! | TAATTTAATAAAATATGTTA+TGG | - | chr4.1:78275188-78275207 | None:intergenic | 5.0% |
| GTTCAGTTTCTTGCCGGGTT+CGG | + | chr4.1:78274793-78274812 | MS.gene73226:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 78274321 | 78275364 | 78274321 | ID=MS.gene73226 |
| chr4.1 | mRNA | 78274321 | 78275364 | 78274321 | ID=MS.gene73226.t1;Parent=MS.gene73226 |
| chr4.1 | exon | 78274321 | 78274353 | 78274321 | ID=MS.gene73226.t1.exon1;Parent=MS.gene73226.t1 |
| chr4.1 | CDS | 78274321 | 78274353 | 78274321 | ID=cds.MS.gene73226.t1;Parent=MS.gene73226.t1 |
| chr4.1 | exon | 78274791 | 78274959 | 78274791 | ID=MS.gene73226.t1.exon2;Parent=MS.gene73226.t1 |
| chr4.1 | CDS | 78274791 | 78274959 | 78274791 | ID=cds.MS.gene73226.t1;Parent=MS.gene73226.t1 |
| chr4.1 | exon | 78275339 | 78275364 | 78275339 | ID=MS.gene73226.t1.exon3;Parent=MS.gene73226.t1 |
| chr4.1 | CDS | 78275339 | 78275364 | 78275339 | ID=cds.MS.gene73226.t1;Parent=MS.gene73226.t1 |
| Gene Sequence |
| Protein sequence |
