Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73269.t1 | RHN58987.1 | 99 | 202 | 2 | 0 | 1 | 202 | 88 | 289 | 4.50E-97 | 364 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73269.t1 | Q9SR77 | 39.7 | 199 | 107 | 5 | 10 | 202 | 114 | 305 | 9.7e-16 | 85.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73269.t1 | G7JRA5 | 99.0 | 202 | 2 | 0 | 1 | 202 | 1 | 202 | 3.2e-97 | 364.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene73269 | 0.81296 | 3.26E-51 | -1.69E-46 |
| MS.gene049151 | MS.gene73269 | 0.82413 | 9.54E-54 | -1.69E-46 |
| MS.gene049153 | MS.gene73269 | 0.821727 | 3.46E-53 | -1.69E-46 |
| MS.gene049260 | MS.gene73269 | 0.808058 | 3.73E-50 | -1.69E-46 |
| MS.gene050128 | MS.gene73269 | 0.852511 | 4.40E-61 | -1.69E-46 |
| MS.gene050301 | MS.gene73269 | 0.802029 | 6.81E-49 | -1.69E-46 |
| MS.gene051983 | MS.gene73269 | 0.807132 | 5.87E-50 | -1.69E-46 |
| MS.gene052340 | MS.gene73269 | 0.824171 | 9.32E-54 | -1.69E-46 |
| MS.gene05316 | MS.gene73269 | 0.836406 | 9.54E-57 | -1.69E-46 |
| MS.gene053494 | MS.gene73269 | 0.804837 | 1.78E-49 | -1.69E-46 |
| MS.gene053555 | MS.gene73269 | 0.830737 | 2.48E-55 | -1.69E-46 |
| MS.gene053557 | MS.gene73269 | 0.805239 | 1.47E-49 | -1.69E-46 |
| MS.gene053559 | MS.gene73269 | 0.806338 | 8.63E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene73269 | 0.80575 | 1.15E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene73269 | 0.803086 | 4.12E-49 | -1.69E-46 |
| MS.gene056447 | MS.gene73269 | 0.807881 | 4.07E-50 | -1.69E-46 |
| MS.gene056723 | MS.gene73269 | 0.823137 | 1.63E-53 | -1.69E-46 |
| MS.gene057448 | MS.gene73269 | 0.804209 | 2.41E-49 | -1.69E-46 |
| MS.gene057449 | MS.gene73269 | 0.804259 | 2.35E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene73269 | 0.803468 | 3.44E-49 | -1.69E-46 |
| MS.gene058002 | MS.gene73269 | 0.802563 | 5.29E-49 | -1.69E-46 |
| MS.gene058142 | MS.gene73269 | 0.809182 | 2.15E-50 | -1.69E-46 |
| MS.gene058833 | MS.gene73269 | 0.808997 | 2.35E-50 | -1.69E-46 |
| MS.gene058949 | MS.gene73269 | 0.808719 | 2.70E-50 | -1.69E-46 |
| MS.gene059788 | MS.gene73269 | 0.839855 | 1.23E-57 | -1.69E-46 |
| MS.gene05984 | MS.gene73269 | 0.806939 | 6.45E-50 | -1.69E-46 |
| MS.gene059899 | MS.gene73269 | 0.830685 | 2.56E-55 | -1.69E-46 |
| MS.gene060495 | MS.gene73269 | 0.806033 | 1.00E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73269.t1 | MTR_4g019150 | 98.515 | 202 | 3 | 0 | 1 | 202 | 1 | 202 | 2.42e-145 | 402 |
| MS.gene73269.t1 | MTR_4g019130 | 74.757 | 206 | 43 | 3 | 1 | 202 | 1 | 201 | 6.14e-106 | 303 |
| MS.gene73269.t1 | MTR_7g104650 | 36.098 | 205 | 106 | 9 | 10 | 202 | 101 | 292 | 7.89e-25 | 98.6 |
| MS.gene73269.t1 | MTR_2g054130 | 33.673 | 196 | 99 | 7 | 10 | 202 | 210 | 377 | 3.00e-19 | 84.7 |
| MS.gene73269.t1 | MTR_2g055010 | 32.642 | 193 | 101 | 6 | 10 | 199 | 210 | 376 | 2.79e-18 | 82.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73269.t1 | AT2G37970 | 69.907 | 216 | 50 | 2 | 1 | 202 | 32 | 246 | 1.32e-103 | 298 |
| MS.gene73269.t1 | AT3G10130 | 35.149 | 202 | 112 | 8 | 10 | 202 | 114 | 305 | 1.93e-19 | 84.7 |
| MS.gene73269.t1 | AT5G20140 | 32.143 | 196 | 103 | 6 | 10 | 202 | 205 | 373 | 4.16e-19 | 84.3 |
| MS.gene73269.t1 | AT5G20140 | 31.351 | 185 | 99 | 5 | 10 | 191 | 205 | 364 | 8.85e-18 | 80.9 |
Find 42 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACAAAGATGGTGGCTTTA+TGG | 0.188087 | 4.1:-79093595 | MS.gene73269:CDS |
| GCTAACTATATTGGAGCATT+AGG | 0.212595 | 4.1:-79093567 | MS.gene73269:CDS |
| TCCATAATGGGTATGGTATT+TGG | 0.221891 | 4.1:-79093738 | None:intergenic |
| GAGGAAGGTGAGAGGAAATA+TGG | 0.245932 | 4.1:-79093327 | MS.gene73269:CDS |
| GGAGAAGTTGAGGCTGAGTT+TGG | 0.256967 | 4.1:-79093253 | MS.gene73269:CDS |
| ACACATCATGTTCATTCAAT+AGG | 0.342036 | 4.1:+79093135 | None:intergenic |
| CTTGGACACTTCCAATGTTT+AGG | 0.347372 | 4.1:-79093176 | MS.gene73269:CDS |
| CTAACTATATTGGAGCATTA+GGG | 0.374151 | 4.1:-79093566 | MS.gene73269:CDS |
| GCAGAACCCTTTGTGATAAC+TGG | 0.387111 | 4.1:+79093501 | None:intergenic |
| CACATCATGTTCATTCAATA+GGG | 0.408624 | 4.1:+79093136 | None:intergenic |
| TTGTTTCCTTTGAACTGTGA+TGG | 0.417876 | 4.1:+79093612 | None:intergenic |
| TCACTAGCCTTAGTCACAAC+TGG | 0.437262 | 4.1:+79093456 | None:intergenic |
| TTTGAACTGTGATGGATCAT+AGG | 0.439692 | 4.1:+79093620 | None:intergenic |
| GACAGCACCAGTTGTGACTA+AGG | 0.455361 | 4.1:-79093463 | MS.gene73269:CDS |
| TATGGAGTGGTGAAGTTTAG+TGG | 0.463832 | 4.1:-79093309 | MS.gene73269:CDS |
| AGAGTTGTGATAAGAGAGGA+AGG | 0.470240 | 4.1:-79093342 | MS.gene73269:CDS |
| TGAAGTAGTGAAGGAGAAGG+TGG | 0.490339 | 4.1:-79093274 | MS.gene73269:CDS |
| AGGCTGAGTTTGGAGAGAGA+TGG | 0.496209 | 4.1:-79093243 | MS.gene73269:CDS |
| TTCTCGATCCATAATGGGTA+TGG | 0.497976 | 4.1:-79093745 | None:intergenic |
| AGGAGAAGGTGGAGAAGTTG+AGG | 0.502041 | 4.1:-79093263 | MS.gene73269:CDS |
| ACTCTCTCATCAGTAGGCTT+AGG | 0.503647 | 4.1:+79093360 | None:intergenic |
| GTGACTAAGGCTAGTGAAGA+AGG | 0.510099 | 4.1:-79093450 | MS.gene73269:CDS |
| TCCAATGTTTAGGACTAATG+AGG | 0.518493 | 4.1:-79093166 | MS.gene73269:CDS |
| TGATAAGAGAGGAAGGTGAG+AGG | 0.519680 | 4.1:-79093335 | MS.gene73269:CDS |
| TGAGAGAGTTGTGATAAGAG+AGG | 0.535873 | 4.1:-79093346 | MS.gene73269:CDS |
| CTAAACATTGGAAGTGTCCA+AGG | 0.551702 | 4.1:+79093177 | None:intergenic |
| TCTGATTTCATAGTCTTGTG+TGG | 0.560504 | 4.1:+79093674 | None:intergenic |
| ACCTCATTAGTCCTAAACAT+TGG | 0.572004 | 4.1:+79093165 | None:intergenic |
| AGAAGGTGAGAAGAATAAGA+TGG | 0.575015 | 4.1:-79093433 | MS.gene73269:CDS |
| ACTGCACCAGTTATCACAAA+GGG | 0.579442 | 4.1:-79093507 | MS.gene73269:CDS |
| TATGATCCATCACAGTTCAA+AGG | 0.581780 | 4.1:-79093618 | MS.gene73269:CDS |
| TGATGAAGTAGTGAAGGAGA+AGG | 0.587311 | 4.1:-79093277 | MS.gene73269:CDS |
| CAGTTCAAAGGAAACAAAGA+TGG | 0.590535 | 4.1:-79093606 | MS.gene73269:CDS |
| GGCAAGTGATGAAGTAGTGA+AGG | 0.598108 | 4.1:-79093283 | MS.gene73269:CDS |
| GTGACTTCAGCTGCTACAGA+AGG | 0.612236 | 4.1:+79093642 | None:intergenic |
| AGGTGAGAGGAAATATGGAG+TGG | 0.613327 | 4.1:-79093322 | MS.gene73269:CDS |
| ATCACAACTCTCTCATCAGT+AGG | 0.625874 | 4.1:+79093354 | None:intergenic |
| TGGGTAGGTACAATCCACCT+TGG | 0.629812 | 4.1:-79093194 | MS.gene73269:CDS |
| TTCAAAGGAAACAAAGATGG+TGG | 0.644336 | 4.1:-79093603 | MS.gene73269:CDS |
| GACTGCACCAGTTATCACAA+AGG | 0.644397 | 4.1:-79093508 | MS.gene73269:CDS |
| AGTGGTGAAGTTTAGTGGTG+TGG | 0.656506 | 4.1:-79093304 | MS.gene73269:CDS |
| AACATTGGAAGTGTCCAAGG+TGG | 0.685808 | 4.1:+79093180 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGGTTTTAGCTAACTATAT+TGG | - | chr4.1:79093302-79093321 | MS.gene73269:CDS | 25.0% |
| !!! | GTTATTGGAGATTTTTTGTT+GGG | - | chr4.1:79093665-79093684 | MS.gene73269:CDS | 25.0% |
| !!! | GTTTTTGTCACTTCATATTT+TGG | + | chr4.1:79093185-79093204 | None:intergenic | 25.0% |
| GGTATGGTATTTGGAAAAAT+TGG | - | chr4.1:79093149-79093168 | MS.gene73269:CDS | 30.0% | |
| ! | CTAACTATATTGGAGCATTA+GGG | - | chr4.1:79093312-79093331 | MS.gene73269:CDS | 30.0% |
| !!! | AGAGATGGTTTTAAGGTTAT+TGG | - | chr4.1:79093650-79093669 | MS.gene73269:CDS | 30.0% |
| !!! | GGTTATTGGAGATTTTTTGT+TGG | - | chr4.1:79093664-79093683 | MS.gene73269:CDS | 30.0% |
| !!! | TTCTTCTGCTTTTTCATACA+TGG | + | chr4.1:79093495-79093514 | None:intergenic | 30.0% |
| ACCTCATTAGTCCTAAACAT+TGG | + | chr4.1:79093716-79093735 | None:intergenic | 35.0% | |
| AGAAGGTGAGAAGAATAAGA+TGG | - | chr4.1:79093445-79093464 | MS.gene73269:CDS | 35.0% | |
| CAGTTCAAAGGAAACAAAGA+TGG | - | chr4.1:79093272-79093291 | MS.gene73269:CDS | 35.0% | |
| TATGATCCATCACAGTTCAA+AGG | - | chr4.1:79093260-79093279 | MS.gene73269:CDS | 35.0% | |
| TTCAAAGGAAACAAAGATGG+TGG | - | chr4.1:79093275-79093294 | MS.gene73269:CDS | 35.0% | |
| TTGTTTCCTTTGAACTGTGA+TGG | + | chr4.1:79093269-79093288 | None:intergenic | 35.0% | |
| ! | AAACAAAGATGGTGGCTTTA+TGG | - | chr4.1:79093283-79093302 | MS.gene73269:CDS | 35.0% |
| ! | GCTAACTATATTGGAGCATT+AGG | - | chr4.1:79093311-79093330 | MS.gene73269:CDS | 35.0% |
| ! | TCCAATGTTTAGGACTAATG+AGG | - | chr4.1:79093712-79093731 | MS.gene73269:CDS | 35.0% |
| !! | TCTGATTTCATAGTCTTGTG+TGG | + | chr4.1:79093207-79093226 | None:intergenic | 35.0% |
| !! | TTTGAACTGTGATGGATCAT+AGG | + | chr4.1:79093261-79093280 | None:intergenic | 35.0% |
| !! | TTTGGAGAGAGATGGTTTTA+AGG | - | chr4.1:79093643-79093662 | MS.gene73269:CDS | 35.0% |
| !!! | TCTGCTTTTTCATACATGGA+GGG | + | chr4.1:79093491-79093510 | None:intergenic | 35.0% |
| !!! | TTCTGCTTTTTCATACATGG+AGG | + | chr4.1:79093492-79093511 | None:intergenic | 35.0% |
| !!! | TTGGAGATTTTTTGTTGGGT+AGG | - | chr4.1:79093669-79093688 | MS.gene73269:CDS | 35.0% |
| !!! | TTTCAGGTTTTGTGTTCTGA+GGG | + | chr4.1:79093340-79093359 | None:intergenic | 35.0% |
| !!! | TTTTCAGGTTTTGTGTTCTG+AGG | + | chr4.1:79093341-79093360 | None:intergenic | 35.0% |
| ACTGCACCAGTTATCACAAA+GGG | - | chr4.1:79093371-79093390 | MS.gene73269:CDS | 40.0% | |
| AGAGTTGTGATAAGAGAGGA+AGG | - | chr4.1:79093536-79093555 | MS.gene73269:CDS | 40.0% | |
| ATCACAACTCTCTCATCAGT+AGG | + | chr4.1:79093527-79093546 | None:intergenic | 40.0% | |
| CTTGGACACTTCCAATGTTT+AGG | - | chr4.1:79093702-79093721 | MS.gene73269:CDS | 40.0% | |
| TATGGAGTGGTGAAGTTTAG+TGG | - | chr4.1:79093569-79093588 | MS.gene73269:CDS | 40.0% | |
| TGAGAGAGTTGTGATAAGAG+AGG | - | chr4.1:79093532-79093551 | MS.gene73269:CDS | 40.0% | |
| TGATGAAGTAGTGAAGGAGA+AGG | - | chr4.1:79093601-79093620 | MS.gene73269:CDS | 40.0% | |
| ! | GCAGTCATAGCAATCTTTTC+AGG | + | chr4.1:79093356-79093375 | None:intergenic | 40.0% |
| !! | CTAAACATTGGAAGTGTCCA+AGG | + | chr4.1:79093704-79093723 | None:intergenic | 40.0% |
| ACTCTCTCATCAGTAGGCTT+AGG | + | chr4.1:79093521-79093540 | None:intergenic | 45.0% | |
| AGGTGAGAGGAAATATGGAG+TGG | - | chr4.1:79093556-79093575 | MS.gene73269:CDS | 45.0% | |
| AGTGGTGAAGTTTAGTGGTG+TGG | - | chr4.1:79093574-79093593 | MS.gene73269:CDS | 45.0% | |
| GACTGCACCAGTTATCACAA+AGG | - | chr4.1:79093370-79093389 | MS.gene73269:CDS | 45.0% | |
| GAGGAAGGTGAGAGGAAATA+TGG | - | chr4.1:79093551-79093570 | MS.gene73269:CDS | 45.0% | |
| GCAGAACCCTTTGTGATAAC+TGG | + | chr4.1:79093380-79093399 | None:intergenic | 45.0% | |
| GGCAAGTGATGAAGTAGTGA+AGG | - | chr4.1:79093595-79093614 | MS.gene73269:CDS | 45.0% | |
| GTGACTAAGGCTAGTGAAGA+AGG | - | chr4.1:79093428-79093447 | MS.gene73269:CDS | 45.0% | |
| TGAAGTAGTGAAGGAGAAGG+TGG | - | chr4.1:79093604-79093623 | MS.gene73269:CDS | 45.0% | |
| TGATAAGAGAGGAAGGTGAG+AGG | - | chr4.1:79093543-79093562 | MS.gene73269:CDS | 45.0% | |
| !! | AACATTGGAAGTGTCCAAGG+TGG | + | chr4.1:79093701-79093720 | None:intergenic | 45.0% |
| !! | TCACTAGCCTTAGTCACAAC+TGG | + | chr4.1:79093425-79093444 | None:intergenic | 45.0% |
| AGGAGAAGGTGGAGAAGTTG+AGG | - | chr4.1:79093615-79093634 | MS.gene73269:CDS | 50.0% | |
| AGGCTGAGTTTGGAGAGAGA+TGG | - | chr4.1:79093635-79093654 | MS.gene73269:CDS | 50.0% | |
| GACAGCACCAGTTGTGACTA+AGG | - | chr4.1:79093415-79093434 | MS.gene73269:CDS | 50.0% | |
| GGAGAAGTTGAGGCTGAGTT+TGG | - | chr4.1:79093625-79093644 | MS.gene73269:CDS | 50.0% | |
| GTGACTTCAGCTGCTACAGA+AGG | + | chr4.1:79093239-79093258 | None:intergenic | 50.0% | |
| TGGGTAGGTACAATCCACCT+TGG | - | chr4.1:79093684-79093703 | MS.gene73269:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 79093146 | 79093754 | 79093146 | ID=MS.gene73269 |
| chr4.1 | mRNA | 79093146 | 79093754 | 79093146 | ID=MS.gene73269.t1;Parent=MS.gene73269 |
| chr4.1 | exon | 79093146 | 79093754 | 79093146 | ID=MS.gene73269.t1.exon1;Parent=MS.gene73269.t1 |
| chr4.1 | CDS | 79093146 | 79093754 | 79093146 | ID=cds.MS.gene73269.t1;Parent=MS.gene73269.t1 |
| Gene Sequence |
| Protein sequence |
