Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73555.t1 | XP_003624883.1 | 96.4 | 110 | 4 | 0 | 1 | 110 | 94 | 203 | 1.40E-47 | 198.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73555.t1 | Q9M612 | 78.2 | 110 | 23 | 1 | 1 | 109 | 95 | 204 | 1.9e-37 | 156.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73555.t1 | G7L4T7 | 96.4 | 110 | 4 | 0 | 1 | 110 | 94 | 203 | 9.8e-48 | 198.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051247 | MS.gene73555 | 0.800889 | 1.17E-48 | -1.69E-46 |
| MS.gene05157 | MS.gene73555 | 0.807695 | 4.46E-50 | -1.69E-46 |
| MS.gene051710 | MS.gene73555 | 0.80713 | 5.88E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene73555 | 0.81436 | 1.60E-51 | -1.69E-46 |
| MS.gene052456 | MS.gene73555 | 0.809769 | 1.61E-50 | -1.69E-46 |
| MS.gene053226 | MS.gene73555 | 0.806357 | 8.56E-50 | -1.69E-46 |
| MS.gene054236 | MS.gene73555 | 0.812887 | 3.38E-51 | -1.69E-46 |
| MS.gene054775 | MS.gene73555 | 0.812607 | 3.89E-51 | -1.69E-46 |
| MS.gene054801 | MS.gene73555 | 0.834285 | 3.27E-56 | -1.69E-46 |
| MS.gene055466 | MS.gene73555 | 0.807216 | 5.63E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene73555 | 0.802226 | 6.20E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73555.t1 | MTR_7g088680 | 96.364 | 110 | 4 | 0 | 1 | 110 | 94 | 203 | 5.15e-72 | 213 |
| MS.gene73555.t1 | MTR_3g092970 | 66.379 | 116 | 33 | 2 | 1 | 110 | 112 | 227 | 1.38e-42 | 139 |
| MS.gene73555.t1 | MTR_3g092980 | 55.285 | 123 | 34 | 2 | 1 | 110 | 104 | 218 | 5.86e-36 | 122 |
| MS.gene73555.t1 | MTR_3g092980 | 55.285 | 123 | 34 | 2 | 1 | 110 | 106 | 220 | 6.58e-36 | 122 |
| MS.gene73555.t1 | MTR_3g093750 | 55.752 | 113 | 37 | 4 | 1 | 110 | 425 | 527 | 9.02e-26 | 100 |
| MS.gene73555.t1 | MTR_3g093720 | 76.744 | 43 | 10 | 0 | 68 | 110 | 215 | 257 | 4.23e-15 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73555.t1 | AT3G12390 | 73.214 | 112 | 28 | 1 | 1 | 110 | 92 | 203 | 2.56e-42 | 138 |
| MS.gene73555.t1 | AT5G13850 | 69.828 | 116 | 28 | 1 | 1 | 109 | 88 | 203 | 1.63e-40 | 133 |
| MS.gene73555.t1 | AT3G49470 | 60.345 | 116 | 40 | 1 | 1 | 110 | 102 | 217 | 7.11e-39 | 129 |
| MS.gene73555.t1 | AT1G33040 | 59.130 | 115 | 41 | 2 | 1 | 109 | 94 | 208 | 1.01e-36 | 124 |
| MS.gene73555.t1 | AT4G10480 | 62.393 | 117 | 34 | 2 | 1 | 109 | 96 | 210 | 5.40e-36 | 122 |
| MS.gene73555.t1 | AT4G10480 | 62.393 | 117 | 34 | 2 | 1 | 109 | 97 | 211 | 5.92e-36 | 122 |
| MS.gene73555.t1 | AT3G49470 | 58.879 | 107 | 38 | 1 | 1 | 101 | 102 | 208 | 1.80e-33 | 116 |
Find 27 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGACACCTACATTATATT+TGG | 0.098409 | 4.4:-64858010 | MS.gene73555:CDS |
| GGAGCAATACCAGAGGATTC+TGG | 0.324111 | 4.4:+64857896 | None:intergenic |
| TCGTCTACATCCTCATCTTC+TGG | 0.324398 | 4.4:+64857875 | None:intergenic |
| CTGACACCTACATTATATTT+GGG | 0.330139 | 4.4:-64858009 | MS.gene73555:CDS |
| GCACCCGCATTGGTCAAATT+AGG | 0.333932 | 4.4:+64857923 | None:intergenic |
| GCTTTAACAGCTCTTGATCT+TGG | 0.356165 | 4.4:+64857800 | None:intergenic |
| AGACATTGTTGCTGCCATTA+TGG | 0.359211 | 4.4:-64857759 | MS.gene73555:CDS |
| GTTGGACTCTTGAAAACATC+TGG | 0.370830 | 4.4:+64858034 | None:intergenic |
| TTAATTTGTTAGCTCCATAA+TGG | 0.425574 | 4.4:+64857745 | None:intergenic |
| CACCACCAACTCAATATCCT+TGG | 0.437037 | 4.4:+64857838 | None:intergenic |
| TTCTGGCTTCGCACCCGCAT+TGG | 0.469966 | 4.4:+64857913 | None:intergenic |
| TCAGGCTGCAGAGCAGTTCA+AGG | 0.493163 | 4.4:-64857948 | MS.gene73555:CDS |
| AAAGCTCTCAAAGCTGTCAA+TGG | 0.508744 | 4.4:-64857782 | MS.gene73555:CDS |
| ATAATGTAGGTGTCAGAAGT+TGG | 0.511639 | 4.4:+64858016 | None:intergenic |
| TCCCAAGGATATTGAGTTGG+TGG | 0.515624 | 4.4:-64857840 | MS.gene73555:CDS |
| GATCCTAATTTGACCAATGC+GGG | 0.519700 | 4.4:-64857926 | MS.gene73555:CDS |
| ACCACCAACTCAATATCCTT+GGG | 0.531886 | 4.4:+64857839 | None:intergenic |
| GGTGCGAAGCCAGAATCCTC+TGG | 0.535151 | 4.4:-64857905 | MS.gene73555:CDS |
| GATGAGGATGTAGACGAGAC+TGG | 0.538671 | 4.4:-64857869 | MS.gene73555:CDS |
| GAGTTGGTGGTGACTCAAGC+TGG | 0.556994 | 4.4:-64857827 | MS.gene73555:CDS |
| AGCTTTCCCAAATATAATGT+AGG | 0.558527 | 4.4:+64858003 | None:intergenic |
| GAGCTCACAGCTACAAACTC+AGG | 0.597975 | 4.4:-64857966 | MS.gene73555:CDS |
| CGAGACTGGTGTAGATCCCA+AGG | 0.607645 | 4.4:-64857855 | MS.gene73555:CDS |
| TGGTATTGCTCCAGAAGATG+AGG | 0.607813 | 4.4:-64857885 | MS.gene73555:CDS |
| AGATCCCAAGGATATTGAGT+TGG | 0.629225 | 4.4:-64857843 | MS.gene73555:CDS |
| ATCTTCTGGAGCAATACCAG+AGG | 0.670950 | 4.4:+64857889 | None:intergenic |
| GGATCCTAATTTGACCAATG+CGG | 0.677685 | 4.4:-64857927 | MS.gene73555:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTAATTTGTTAGCTCCATAA+TGG | + | chr4.4:64858058-64858077 | None:intergenic | 25.0% |
| AGCTTTCCCAAATATAATGT+AGG | + | chr4.4:64857800-64857819 | None:intergenic | 30.0% | |
| CTGACACCTACATTATATTT+GGG | - | chr4.4:64857791-64857810 | MS.gene73555:CDS | 30.0% | |
| TCTGACACCTACATTATATT+TGG | - | chr4.4:64857790-64857809 | MS.gene73555:CDS | 30.0% | |
| ATAATGTAGGTGTCAGAAGT+TGG | + | chr4.4:64857787-64857806 | None:intergenic | 35.0% | |
| AAAGCTCTCAAAGCTGTCAA+TGG | - | chr4.4:64858018-64858037 | MS.gene73555:CDS | 40.0% | |
| ACCACCAACTCAATATCCTT+GGG | + | chr4.4:64857964-64857983 | None:intergenic | 40.0% | |
| AGACATTGTTGCTGCCATTA+TGG | - | chr4.4:64858041-64858060 | MS.gene73555:CDS | 40.0% | |
| AGATCCCAAGGATATTGAGT+TGG | - | chr4.4:64857957-64857976 | MS.gene73555:CDS | 40.0% | |
| GATCCTAATTTGACCAATGC+GGG | - | chr4.4:64857874-64857893 | MS.gene73555:CDS | 40.0% | |
| GCTTTAACAGCTCTTGATCT+TGG | + | chr4.4:64858003-64858022 | None:intergenic | 40.0% | |
| GGATCCTAATTTGACCAATG+CGG | - | chr4.4:64857873-64857892 | MS.gene73555:CDS | 40.0% | |
| GTTGGACTCTTGAAAACATC+TGG | + | chr4.4:64857769-64857788 | None:intergenic | 40.0% | |
| CACCACCAACTCAATATCCT+TGG | + | chr4.4:64857965-64857984 | None:intergenic | 45.0% | |
| TCGTCTACATCCTCATCTTC+TGG | + | chr4.4:64857928-64857947 | None:intergenic | 45.0% | |
| TGGTATTGCTCCAGAAGATG+AGG | - | chr4.4:64857915-64857934 | MS.gene73555:CDS | 45.0% | |
| !! | ATCTTCTGGAGCAATACCAG+AGG | + | chr4.4:64857914-64857933 | None:intergenic | 45.0% |
| !! | TCCCAAGGATATTGAGTTGG+TGG | - | chr4.4:64857960-64857979 | MS.gene73555:CDS | 45.0% |
| GAGCTCACAGCTACAAACTC+AGG | - | chr4.4:64857834-64857853 | MS.gene73555:CDS | 50.0% | |
| GATGAGGATGTAGACGAGAC+TGG | - | chr4.4:64857931-64857950 | MS.gene73555:CDS | 50.0% | |
| GGAGCAATACCAGAGGATTC+TGG | + | chr4.4:64857907-64857926 | None:intergenic | 50.0% | |
| ! | GCACCCGCATTGGTCAAATT+AGG | + | chr4.4:64857880-64857899 | None:intergenic | 50.0% |
| CGAGACTGGTGTAGATCCCA+AGG | - | chr4.4:64857945-64857964 | MS.gene73555:CDS | 55.0% | |
| TCAGGCTGCAGAGCAGTTCA+AGG | - | chr4.4:64857852-64857871 | MS.gene73555:CDS | 55.0% | |
| !! | GAGTTGGTGGTGACTCAAGC+TGG | - | chr4.4:64857973-64857992 | MS.gene73555:CDS | 55.0% |
| GGTGCGAAGCCAGAATCCTC+TGG | - | chr4.4:64857895-64857914 | MS.gene73555:CDS | 60.0% | |
| !! | TTCTGGCTTCGCACCCGCAT+TGG | + | chr4.4:64857890-64857909 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 64857745 | 64858077 | 64857745 | ID=MS.gene73555 |
| chr4.4 | mRNA | 64857745 | 64858077 | 64857745 | ID=MS.gene73555.t1;Parent=MS.gene73555 |
| chr4.4 | exon | 64857745 | 64858077 | 64857745 | ID=MS.gene73555.t1.exon1;Parent=MS.gene73555.t1 |
| chr4.4 | CDS | 64857745 | 64858077 | 64857745 | ID=cds.MS.gene73555.t1;Parent=MS.gene73555.t1 |
| Gene Sequence |
| Protein sequence |
