Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73626.t1 | RHN60424.1 | 93.4 | 212 | 14 | 0 | 1 | 212 | 1 | 212 | 5.60E-106 | 393.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73626.t1 | A0A396I855 | 93.4 | 212 | 14 | 0 | 1 | 212 | 1 | 212 | 4.0e-106 | 393.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene73626 | 0.837749 | 4.32E-57 | -1.69E-46 |
| MS.gene050759 | MS.gene73626 | 0.822425 | 2.38E-53 | -1.69E-46 |
| MS.gene050962 | MS.gene73626 | 0.809614 | 1.73E-50 | -1.69E-46 |
| MS.gene051198 | MS.gene73626 | 0.800355 | 1.50E-48 | -1.69E-46 |
| MS.gene051800 | MS.gene73626 | 0.807181 | 5.73E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene73626 | 0.803547 | 3.31E-49 | -1.69E-46 |
| MS.gene053008 | MS.gene73626 | 0.812922 | 3.32E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene73626 | 0.821075 | 4.90E-53 | -1.69E-46 |
| MS.gene055926 | MS.gene73626 | 0.802846 | 4.62E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene73626 | 0.803129 | 4.04E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene73626 | 0.807761 | 4.32E-50 | -1.69E-46 |
| MS.gene058010 | MS.gene73626 | 0.819546 | 1.10E-52 | -1.69E-46 |
| MS.gene058625 | MS.gene73626 | 0.804516 | 2.08E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene73626 | 0.80465 | 1.95E-49 | -1.69E-46 |
| MS.gene060011 | MS.gene73626 | 0.818187 | 2.23E-52 | -1.69E-46 |
| MS.gene06052 | MS.gene73626 | 0.823914 | 1.07E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73626.t1 | MTR_4g051652 | 93.333 | 210 | 14 | 0 | 3 | 212 | 1 | 210 | 4.34e-145 | 402 |
| MS.gene73626.t1 | MTR_2g087080 | 53.365 | 208 | 91 | 3 | 5 | 206 | 4 | 211 | 3.33e-68 | 208 |
| MS.gene73626.t1 | MTR_4g051672 | 54.930 | 213 | 23 | 5 | 3 | 212 | 1 | 143 | 7.25e-64 | 194 |
| MS.gene73626.t1 | MTR_4g051705 | 91.011 | 89 | 8 | 0 | 124 | 212 | 1 | 89 | 1.74e-54 | 169 |
| MS.gene73626.t1 | MTR_4g051745 | 82.022 | 89 | 4 | 1 | 124 | 212 | 1 | 77 | 6.11e-45 | 144 |
| MS.gene73626.t1 | MTR_2g087000 | 27.136 | 199 | 138 | 3 | 9 | 207 | 39 | 230 | 2.58e-22 | 90.9 |
| MS.gene73626.t1 | MTR_2g011350 | 27.273 | 154 | 108 | 3 | 41 | 193 | 41 | 191 | 3.12e-12 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73626.t1 | AT3G52460 | 30.412 | 194 | 124 | 4 | 14 | 199 | 99 | 289 | 4.76e-18 | 80.9 |
| MS.gene73626.t1 | AT2G27260 | 27.381 | 168 | 103 | 4 | 46 | 205 | 84 | 240 | 2.25e-11 | 61.6 |
| MS.gene73626.t1 | AT5G22870 | 25.389 | 193 | 133 | 6 | 3 | 187 | 1 | 190 | 4.80e-11 | 60.5 |
Find 52 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCGATGTGGAGATACTTTA+CGG | 0.300931 | 4.1:-46341392 | MS.gene73626:CDS |
| AAAAGACACTGGAAAACTAT+TGG | 0.316716 | 4.1:-46341096 | MS.gene73626:CDS |
| TTCAATAACTAACTATGGTT+TGG | 0.319426 | 4.1:+46341043 | None:intergenic |
| TTGGATTTGAAGCTGGAATA+TGG | 0.322794 | 4.1:-46341181 | MS.gene73626:CDS |
| CTGGAAAACTATTGGGTATT+GGG | 0.345902 | 4.1:-46341088 | MS.gene73626:CDS |
| ATGTGTTTCTTGTGGGGACT+TGG | 0.423348 | 4.1:-46341138 | MS.gene73626:CDS |
| ACTGGAAAACTATTGGGTAT+TGG | 0.428456 | 4.1:-46341089 | MS.gene73626:CDS |
| AAAGACACTGGAAAACTATT+GGG | 0.440360 | 4.1:-46341095 | MS.gene73626:CDS |
| GGTGCAACAACCTATACTTT+TGG | 0.440362 | 4.1:-46341345 | MS.gene73626:CDS |
| TGGCCATCATTGGCATAGCT+TGG | 0.441176 | 4.1:-46341565 | MS.gene73626:CDS |
| CTTCCGACACCGGCTTTGTC+CGG | 0.445859 | 4.1:-46341628 | MS.gene73626:CDS |
| TTCTTGTGGGGACTTGGATG+TGG | 0.458190 | 4.1:-46341132 | MS.gene73626:CDS |
| GTTAGAGTTGGATTTGAAGC+TGG | 0.458770 | 4.1:-46341188 | MS.gene73626:CDS |
| GTAAAGATGGTTGTTAGAGT+TGG | 0.462015 | 4.1:-46341200 | MS.gene73626:CDS |
| CTCATCTTCACCAAAAGTAT+AGG | 0.463962 | 4.1:+46341335 | None:intergenic |
| ACAGATTACAGGCGTGTTCG+AGG | 0.475631 | 4.1:-46341468 | MS.gene73626:CDS |
| AGTGATGTTACCGCAAAACT+AGG | 0.482969 | 4.1:+46341521 | None:intergenic |
| GTTGGGATATTTCTAGTAGG+AGG | 0.485932 | 4.1:+46341656 | None:intergenic |
| CAAGAGTATGAAGGTTGACT+TGG | 0.487527 | 4.1:-46341309 | MS.gene73626:CDS |
| CGGTGTCGGAAGATCTTGTT+GGG | 0.487634 | 4.1:+46341639 | None:intergenic |
| AAGACATTATGTGTTTCTTG+TGG | 0.490786 | 4.1:-46341146 | MS.gene73626:CDS |
| TTGGGATATTTCTAGTAGGA+GGG | 0.498040 | 4.1:+46341657 | None:intergenic |
| CTTGTTGGGATATTTCTAGT+AGG | 0.499013 | 4.1:+46341653 | None:intergenic |
| AGACATTATGTGTTTCTTGT+GGG | 0.505280 | 4.1:-46341145 | MS.gene73626:CDS |
| GTATTCGATTCACAGATTAC+AGG | 0.518278 | 4.1:-46341479 | MS.gene73626:CDS |
| TAGTTGCGGAGTGGAACAAA+GGG | 0.526316 | 4.1:-46341238 | MS.gene73626:CDS |
| CCGGTGTCGGAAGATCTTGT+TGG | 0.532501 | 4.1:+46341638 | None:intergenic |
| AAACCAAGCTATGCCAATGA+TGG | 0.532510 | 4.1:+46341562 | None:intergenic |
| ATTACAGGCGTGTTCGAGGC+GGG | 0.548697 | 4.1:-46341464 | MS.gene73626:CDS |
| GACATTATGTGTTTCTTGTG+GGG | 0.561691 | 4.1:-46341144 | MS.gene73626:CDS |
| GGTTAGACGCCGGACAAAGC+CGG | 0.562191 | 4.1:+46341619 | None:intergenic |
| TTACGGTGAAACTGTGCTCT+CGG | 0.565164 | 4.1:-46341375 | MS.gene73626:CDS |
| TTCTATTCTACAAAAGACAC+TGG | 0.567750 | 4.1:-46341107 | MS.gene73626:CDS |
| AACGAAGTTCTTGCAGAAGG+TGG | 0.568702 | 4.1:-46341273 | MS.gene73626:CDS |
| AACTGTGCTCTCGGAAGCTG+AGG | 0.572206 | 4.1:-46341366 | MS.gene73626:CDS |
| TTCAACGAAGTTCTTGCAGA+AGG | 0.572364 | 4.1:-46341276 | MS.gene73626:CDS |
| ACGCCGGACAAAGCCGGTGT+CGG | 0.579838 | 4.1:+46341625 | None:intergenic |
| CTAGTTGCGGAGTGGAACAA+AGG | 0.586760 | 4.1:-46341239 | MS.gene73626:CDS |
| GATGAGCAACAAGAGTATGA+AGG | 0.586934 | 4.1:-46341318 | MS.gene73626:CDS |
| AGCTGGAATATGGCCAAGCA+AGG | 0.592271 | 4.1:-46341171 | MS.gene73626:CDS |
| GTGATGTTACCGCAAAACTA+GGG | 0.599545 | 4.1:+46341522 | None:intergenic |
| AAAGATTGATACAACTGTCA+AGG | 0.607863 | 4.1:+46341598 | None:intergenic |
| CCAACAAGATCTTCCGACAC+CGG | 0.612704 | 4.1:-46341638 | MS.gene73626:CDS |
| AAAATCCAAAGTAATGAACA+TGG | 0.633536 | 4.1:-46341684 | None:intergenic |
| ACCGCAAAACTAGGGTCGTG+TGG | 0.643655 | 4.1:+46341530 | None:intergenic |
| GGTTCTCGATAATTTCGATG+TGG | 0.655826 | 4.1:-46341405 | MS.gene73626:CDS |
| TGCGGAGTGGAACAAAGGGG+TGG | 0.672319 | 4.1:-46341234 | MS.gene73626:CDS |
| GGTGCAAGAGAATCTAGTTG+CGG | 0.673473 | 4.1:-46341252 | MS.gene73626:CDS |
| GATTACAGGCGTGTTCGAGG+CGG | 0.683167 | 4.1:-46341465 | MS.gene73626:CDS |
| AAGAGAATCTAGTTGCGGAG+TGG | 0.723007 | 4.1:-46341247 | MS.gene73626:CDS |
| CAACTGTCAAGGTTAGACGC+CGG | 0.742495 | 4.1:+46341609 | None:intergenic |
| AGTTGCGGAGTGGAACAAAG+GGG | 0.746823 | 4.1:-46341237 | MS.gene73626:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTGTTTTGGTTTGTTA+TGG | + | chr4.1:46341293-46341312 | None:intergenic | 20.0% |
| ! | CCAAAACAAAAAAATCGAAT+TGG | - | chr4.1:46341300-46341319 | MS.gene73626:CDS | 25.0% |
| !!! | ATCAATCTTTGTCATTTTCA+TGG | - | chr4.1:46341141-46341160 | MS.gene73626:CDS | 25.0% |
| !!! | CCAATTCGATTTTTTTGTTT+TGG | + | chr4.1:46341303-46341322 | None:intergenic | 25.0% |
| AAAAGACACTGGAAAACTAT+TGG | - | chr4.1:46341630-46341649 | MS.gene73626:CDS | 30.0% | |
| AAAGACACTGGAAAACTATT+GGG | - | chr4.1:46341631-46341650 | MS.gene73626:CDS | 30.0% | |
| AAAGATTGATACAACTGTCA+AGG | + | chr4.1:46341131-46341150 | None:intergenic | 30.0% | |
| AAGACATTATGTGTTTCTTG+TGG | - | chr4.1:46341580-46341599 | MS.gene73626:CDS | 30.0% | |
| AGACATTATGTGTTTCTTGT+GGG | - | chr4.1:46341581-46341600 | MS.gene73626:CDS | 30.0% | |
| TTCTATTCTACAAAAGACAC+TGG | - | chr4.1:46341619-46341638 | MS.gene73626:CDS | 30.0% | |
| !! | GGTGAATTTTGATGTAAAGA+TGG | - | chr4.1:46341513-46341532 | MS.gene73626:CDS | 30.0% |
| ACTGGAAAACTATTGGGTAT+TGG | - | chr4.1:46341637-46341656 | MS.gene73626:CDS | 35.0% | |
| CTGGAAAACTATTGGGTATT+GGG | - | chr4.1:46341638-46341657 | MS.gene73626:CDS | 35.0% | |
| GACATTATGTGTTTCTTGTG+GGG | - | chr4.1:46341582-46341601 | MS.gene73626:CDS | 35.0% | |
| GTAAAGATGGTTGTTAGAGT+TGG | - | chr4.1:46341526-46341545 | MS.gene73626:CDS | 35.0% | |
| GTATTCGATTCACAGATTAC+AGG | - | chr4.1:46341247-46341266 | MS.gene73626:CDS | 35.0% | |
| TTCGATGTGGAGATACTTTA+CGG | - | chr4.1:46341334-46341353 | MS.gene73626:CDS | 35.0% | |
| ! | CATAATGTCTTTTCCTTGCT+TGG | + | chr4.1:46341571-46341590 | None:intergenic | 35.0% |
| ! | CTCATCTTCACCAAAAGTAT+AGG | + | chr4.1:46341394-46341413 | None:intergenic | 35.0% |
| ! | TTGGATTTGAAGCTGGAATA+TGG | - | chr4.1:46341545-46341564 | MS.gene73626:CDS | 35.0% |
| !! | CTTGTTGGGATATTTCTAGT+AGG | + | chr4.1:46341076-46341095 | None:intergenic | 35.0% |
| !! | TTGGGATATTTCTAGTAGGA+GGG | + | chr4.1:46341072-46341091 | None:intergenic | 35.0% |
| AAACCAAGCTATGCCAATGA+TGG | + | chr4.1:46341167-46341186 | None:intergenic | 40.0% | |
| AGTGATGTTACCGCAAAACT+AGG | + | chr4.1:46341208-46341227 | None:intergenic | 40.0% | |
| GGTTCTCGATAATTTCGATG+TGG | - | chr4.1:46341321-46341340 | MS.gene73626:CDS | 40.0% | |
| GTGATGTTACCGCAAAACTA+GGG | + | chr4.1:46341207-46341226 | None:intergenic | 40.0% | |
| TTCAACGAAGTTCTTGCAGA+AGG | - | chr4.1:46341450-46341469 | MS.gene73626:CDS | 40.0% | |
| ! | CAAGAGTATGAAGGTTGACT+TGG | - | chr4.1:46341417-46341436 | MS.gene73626:CDS | 40.0% |
| ! | GATGAGCAACAAGAGTATGA+AGG | - | chr4.1:46341408-46341427 | MS.gene73626:CDS | 40.0% |
| ! | GGTGCAACAACCTATACTTT+TGG | - | chr4.1:46341381-46341400 | MS.gene73626:CDS | 40.0% |
| ! | GTTAGAGTTGGATTTGAAGC+TGG | - | chr4.1:46341538-46341557 | MS.gene73626:CDS | 40.0% |
| !! | GTCATTTTCATGGCCATCAT+TGG | - | chr4.1:46341151-46341170 | MS.gene73626:CDS | 40.0% |
| !! | GTTGGGATATTTCTAGTAGG+AGG | + | chr4.1:46341073-46341092 | None:intergenic | 40.0% |
| AACGAAGTTCTTGCAGAAGG+TGG | - | chr4.1:46341453-46341472 | MS.gene73626:CDS | 45.0% | |
| AAGAGAATCTAGTTGCGGAG+TGG | - | chr4.1:46341479-46341498 | MS.gene73626:CDS | 45.0% | |
| ATGTGTTTCTTGTGGGGACT+TGG | - | chr4.1:46341588-46341607 | MS.gene73626:CDS | 45.0% | |
| GGTGCAAGAGAATCTAGTTG+CGG | - | chr4.1:46341474-46341493 | MS.gene73626:CDS | 45.0% | |
| TAGTTGCGGAGTGGAACAAA+GGG | - | chr4.1:46341488-46341507 | MS.gene73626:CDS | 45.0% | |
| TTACGGTGAAACTGTGCTCT+CGG | - | chr4.1:46341351-46341370 | MS.gene73626:CDS | 45.0% | |
| ACAGATTACAGGCGTGTTCG+AGG | - | chr4.1:46341258-46341277 | MS.gene73626:CDS | 50.0% | |
| AGCTGGAATATGGCCAAGCA+AGG | - | chr4.1:46341555-46341574 | MS.gene73626:CDS | 50.0% | |
| AGTTGCGGAGTGGAACAAAG+GGG | - | chr4.1:46341489-46341508 | MS.gene73626:CDS | 50.0% | |
| CAACTGTCAAGGTTAGACGC+CGG | + | chr4.1:46341120-46341139 | None:intergenic | 50.0% | |
| CCAACAAGATCTTCCGACAC+CGG | - | chr4.1:46341088-46341107 | MS.gene73626:CDS | 50.0% | |
| CTAGTTGCGGAGTGGAACAA+AGG | - | chr4.1:46341487-46341506 | MS.gene73626:CDS | 50.0% | |
| TGGCCATCATTGGCATAGCT+TGG | - | chr4.1:46341161-46341180 | MS.gene73626:CDS | 50.0% | |
| TTCTTGTGGGGACTTGGATG+TGG | - | chr4.1:46341594-46341613 | MS.gene73626:CDS | 50.0% | |
| ! | CGGTGTCGGAAGATCTTGTT+GGG | + | chr4.1:46341090-46341109 | None:intergenic | 50.0% |
| !!! | TCCACACGACCCTAGTTTTG+CGG | - | chr4.1:46341195-46341214 | MS.gene73626:CDS | 50.0% |
| AACTGTGCTCTCGGAAGCTG+AGG | - | chr4.1:46341360-46341379 | MS.gene73626:CDS | 55.0% | |
| ACCGCAAAACTAGGGTCGTG+TGG | + | chr4.1:46341199-46341218 | None:intergenic | 55.0% | |
| ATTACAGGCGTGTTCGAGGC+GGG | - | chr4.1:46341262-46341281 | MS.gene73626:CDS | 55.0% | |
| GATTACAGGCGTGTTCGAGG+CGG | - | chr4.1:46341261-46341280 | MS.gene73626:CDS | 55.0% | |
| ! | CCGGTGTCGGAAGATCTTGT+TGG | + | chr4.1:46341091-46341110 | None:intergenic | 55.0% |
| GGTTAGACGCCGGACAAAGC+CGG | + | chr4.1:46341110-46341129 | None:intergenic | 60.0% | |
| TGCGGAGTGGAACAAAGGGG+TGG | - | chr4.1:46341492-46341511 | MS.gene73626:CDS | 60.0% | |
| !! | CTTCCGACACCGGCTTTGTC+CGG | - | chr4.1:46341098-46341117 | MS.gene73626:CDS | 60.0% |
| ACGCCGGACAAAGCCGGTGT+CGG | + | chr4.1:46341104-46341123 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 46341055 | 46341693 | 46341055 | ID=MS.gene73626 |
| chr4.1 | mRNA | 46341055 | 46341693 | 46341055 | ID=MS.gene73626.t1;Parent=MS.gene73626 |
| chr4.1 | exon | 46341055 | 46341693 | 46341055 | ID=MS.gene73626.t1.exon1;Parent=MS.gene73626.t1 |
| chr4.1 | CDS | 46341055 | 46341693 | 46341055 | ID=cds.MS.gene73626.t1;Parent=MS.gene73626.t1 |
| Gene Sequence |
| Protein sequence |
