Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74137.t1 | RHN44832.1 | 84.4 | 314 | 49 | 0 | 1 | 314 | 226 | 539 | 2.80E-138 | 501.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74137.t1 | P09189 | 59.5 | 311 | 123 | 3 | 1 | 308 | 313 | 623 | 3.4e-92 | 339.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74137.t1 | G7KZY7 | 84.4 | 314 | 49 | 0 | 1 | 314 | 315 | 628 | 2.0e-138 | 501.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene74137 | 0.809967 | 1.46E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene74137 | 0.807254 | 5.53E-50 | -1.69E-46 |
| MS.gene052584 | MS.gene74137 | 0.807041 | 6.13E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene74137 | 0.822468 | 2.33E-53 | -1.69E-46 |
| MS.gene055420 | MS.gene74137 | 0.807438 | 5.06E-50 | -1.69E-46 |
| MS.gene056050 | MS.gene74137 | 0.800233 | 1.59E-48 | -1.69E-46 |
| MS.gene056595 | MS.gene74137 | 0.812547 | 4.01E-51 | -1.69E-46 |
| MS.gene057444 | MS.gene74137 | 0.840314 | 9.37E-58 | -1.69E-46 |
| MS.gene058625 | MS.gene74137 | 0.812625 | 3.86E-51 | -1.69E-46 |
| MS.gene058979 | MS.gene74137 | 0.813672 | 2.27E-51 | -1.69E-46 |
| MS.gene059625 | MS.gene74137 | 0.821343 | 4.25E-53 | -1.69E-46 |
| MS.gene060572 | MS.gene74137 | 0.805837 | 1.10E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74137.t1 | MTR_7g025720 | 84.868 | 304 | 46 | 0 | 1 | 304 | 315 | 618 | 0.0 | 522 |
| MS.gene74137.t1 | MTR_7g025630 | 84.868 | 304 | 46 | 0 | 1 | 304 | 301 | 604 | 0.0 | 516 |
| MS.gene74137.t1 | MTR_7g026030 | 75.399 | 313 | 77 | 0 | 1 | 313 | 315 | 627 | 1.77e-167 | 479 |
| MS.gene74137.t1 | MTR_8g032650 | 76.115 | 314 | 75 | 0 | 1 | 314 | 315 | 628 | 1.08e-165 | 475 |
| MS.gene74137.t1 | MTR_7g025760 | 76.721 | 305 | 70 | 1 | 1 | 304 | 315 | 619 | 4.06e-164 | 471 |
| MS.gene74137.t1 | MTR_7g025260 | 69.524 | 315 | 95 | 1 | 1 | 314 | 287 | 601 | 5.31e-153 | 442 |
| MS.gene74137.t1 | MTR_7g025740 | 70.312 | 320 | 86 | 3 | 1 | 311 | 315 | 634 | 4.19e-149 | 433 |
| MS.gene74137.t1 | MTR_7g025790 | 71.667 | 300 | 85 | 0 | 2 | 301 | 316 | 615 | 2.82e-148 | 430 |
| MS.gene74137.t1 | MTR_7g078190 | 69.156 | 308 | 92 | 2 | 1 | 305 | 316 | 623 | 1.49e-144 | 421 |
| MS.gene74137.t1 | MTR_7g078210 | 69.055 | 307 | 92 | 2 | 1 | 304 | 315 | 621 | 1.12e-143 | 419 |
| MS.gene74137.t1 | MTR_7g024030 | 70.134 | 298 | 89 | 0 | 1 | 298 | 315 | 612 | 2.58e-143 | 417 |
| MS.gene74137.t1 | MTR_7g025620 | 68.792 | 298 | 93 | 0 | 1 | 298 | 315 | 612 | 6.15e-138 | 404 |
| MS.gene74137.t1 | MTR_7g025820 | 67.491 | 283 | 91 | 1 | 1 | 282 | 315 | 597 | 2.66e-129 | 381 |
| MS.gene74137.t1 | MTR_7g021440 | 62.207 | 299 | 112 | 1 | 1 | 298 | 315 | 613 | 6.99e-119 | 355 |
| MS.gene74137.t1 | MTR_4g063720 | 60.811 | 296 | 113 | 3 | 1 | 293 | 242 | 537 | 8.72e-118 | 351 |
| MS.gene74137.t1 | MTR_7g026090 | 61.565 | 294 | 110 | 2 | 1 | 293 | 315 | 606 | 8.74e-118 | 352 |
| MS.gene74137.t1 | MTR_7g024390 | 59.539 | 304 | 120 | 3 | 1 | 301 | 313 | 616 | 1.08e-117 | 353 |
| MS.gene74137.t1 | MTR_4g063710 | 61.149 | 296 | 112 | 3 | 1 | 293 | 313 | 608 | 3.26e-117 | 352 |
| MS.gene74137.t1 | MTR_4g063720 | 60.811 | 296 | 113 | 3 | 1 | 293 | 313 | 608 | 4.71e-117 | 351 |
| MS.gene74137.t1 | MTR_7g011590 | 62.366 | 279 | 105 | 0 | 1 | 279 | 303 | 581 | 2.14e-116 | 348 |
| MS.gene74137.t1 | MTR_7g074460 | 58.746 | 303 | 124 | 1 | 1 | 302 | 297 | 599 | 3.03e-116 | 348 |
| MS.gene74137.t1 | MTR_7g024580 | 58.632 | 307 | 124 | 3 | 1 | 304 | 313 | 619 | 3.94e-116 | 349 |
| MS.gene74137.t1 | MTR_7g026110 | 57.333 | 300 | 128 | 0 | 1 | 300 | 314 | 613 | 7.06e-116 | 347 |
| MS.gene74137.t1 | MTR_7g025770 | 59.934 | 302 | 119 | 2 | 1 | 300 | 315 | 616 | 1.13e-115 | 347 |
| MS.gene74137.t1 | MTR_7g451380 | 59.406 | 303 | 120 | 2 | 1 | 300 | 315 | 617 | 2.22e-115 | 346 |
| MS.gene74137.t1 | MTR_2g032550 | 59.344 | 305 | 121 | 3 | 1 | 302 | 316 | 620 | 2.30e-114 | 343 |
| MS.gene74137.t1 | MTR_7g099680 | 57.655 | 307 | 127 | 3 | 1 | 304 | 313 | 619 | 5.49e-114 | 343 |
| MS.gene74137.t1 | MTR_5g066800 | 58.804 | 301 | 123 | 1 | 1 | 300 | 326 | 626 | 2.34e-113 | 341 |
| MS.gene74137.t1 | MTR_2g038270 | 59.076 | 303 | 121 | 3 | 1 | 300 | 323 | 625 | 5.96e-113 | 340 |
| MS.gene74137.t1 | MTR_8g103930 | 58.278 | 302 | 125 | 1 | 1 | 301 | 312 | 613 | 8.13e-112 | 337 |
| MS.gene74137.t1 | MTR_4g130540 | 55.769 | 312 | 135 | 3 | 1 | 309 | 312 | 623 | 6.41e-111 | 336 |
| MS.gene74137.t1 | MTR_1g017130 | 58.784 | 296 | 120 | 2 | 1 | 295 | 277 | 571 | 8.22e-111 | 333 |
| MS.gene74137.t1 | MTR_4g079590 | 58.586 | 297 | 121 | 2 | 1 | 295 | 315 | 611 | 1.17e-110 | 334 |
| MS.gene74137.t1 | MTR_2g038260 | 58.416 | 303 | 122 | 4 | 1 | 300 | 315 | 616 | 1.70e-108 | 328 |
| MS.gene74137.t1 | MTR_2g022050 | 56.766 | 303 | 128 | 3 | 1 | 300 | 315 | 617 | 8.53e-105 | 319 |
| MS.gene74137.t1 | MTR_7g026170 | 53.947 | 304 | 138 | 2 | 1 | 302 | 315 | 618 | 3.73e-100 | 307 |
| MS.gene74137.t1 | MTR_4g027145 | 53.947 | 304 | 138 | 2 | 1 | 302 | 327 | 630 | 1.49e-99 | 306 |
| MS.gene74137.t1 | MTR_1g041565 | 54.698 | 298 | 116 | 2 | 1 | 296 | 258 | 538 | 3.78e-99 | 302 |
| MS.gene74137.t1 | MTR_7g029415 | 54.934 | 304 | 134 | 3 | 1 | 302 | 322 | 624 | 1.56e-98 | 303 |
| MS.gene74137.t1 | MTR_7g026050 | 55.034 | 298 | 104 | 4 | 1 | 295 | 71 | 341 | 2.01e-98 | 294 |
| MS.gene74137.t1 | MTR_7g026140 | 53.333 | 315 | 141 | 5 | 1 | 312 | 299 | 610 | 1.63e-97 | 300 |
| MS.gene74137.t1 | MTR_2g437440 | 53.795 | 303 | 119 | 4 | 1 | 300 | 315 | 599 | 1.82e-95 | 294 |
| MS.gene74137.t1 | MTR_1g077240 | 55.593 | 295 | 122 | 3 | 8 | 300 | 1 | 288 | 2.77e-93 | 279 |
| MS.gene74137.t1 | MTR_7g451360 | 51.155 | 303 | 116 | 3 | 1 | 300 | 285 | 558 | 1.85e-90 | 280 |
| MS.gene74137.t1 | MTR_7g026130 | 53.667 | 300 | 132 | 3 | 1 | 294 | 315 | 613 | 1.66e-88 | 279 |
| MS.gene74137.t1 | MTR_2g032580 | 52.303 | 304 | 109 | 7 | 1 | 300 | 315 | 586 | 1.54e-86 | 271 |
| MS.gene74137.t1 | MTR_8g099795 | 50.179 | 279 | 135 | 4 | 2 | 276 | 340 | 618 | 7.73e-85 | 268 |
| MS.gene74137.t1 | MTR_8g099945 | 49.462 | 279 | 137 | 4 | 2 | 276 | 340 | 618 | 7.74e-84 | 266 |
| MS.gene74137.t1 | MTR_6g033760 | 47.134 | 314 | 155 | 3 | 4 | 306 | 316 | 629 | 8.67e-84 | 265 |
| MS.gene74137.t1 | MTR_6g033800 | 45.847 | 301 | 160 | 3 | 4 | 302 | 227 | 526 | 1.29e-81 | 258 |
| MS.gene74137.t1 | MTR_6g033850 | 46.026 | 302 | 160 | 3 | 4 | 302 | 316 | 617 | 1.41e-81 | 260 |
| MS.gene74137.t1 | MTR_6g033870 | 45.687 | 313 | 163 | 3 | 3 | 308 | 317 | 629 | 9.33e-80 | 255 |
| MS.gene74137.t1 | MTR_6g033820 | 48.327 | 269 | 132 | 3 | 3 | 264 | 315 | 583 | 3.89e-79 | 254 |
| MS.gene74137.t1 | MTR_7g007000 | 48.829 | 299 | 123 | 4 | 1 | 295 | 297 | 569 | 5.64e-79 | 251 |
| MS.gene74137.t1 | MTR_2g017730 | 51.793 | 251 | 114 | 4 | 2 | 245 | 315 | 565 | 8.76e-78 | 248 |
| MS.gene74137.t1 | MTR_6g033955 | 47.584 | 269 | 134 | 3 | 3 | 264 | 315 | 583 | 9.90e-73 | 236 |
| MS.gene74137.t1 | MTR_7g035155 | 51.230 | 244 | 116 | 3 | 61 | 302 | 322 | 564 | 3.24e-65 | 215 |
| MS.gene74137.t1 | MTR_2g010020 | 46.122 | 245 | 125 | 5 | 2 | 244 | 356 | 595 | 1.14e-62 | 210 |
| MS.gene74137.t1 | MTR_2g010020 | 46.122 | 245 | 125 | 5 | 2 | 244 | 357 | 596 | 1.27e-62 | 210 |
| MS.gene74137.t1 | MTR_4g092920 | 46.531 | 245 | 124 | 5 | 2 | 244 | 220 | 459 | 5.36e-62 | 206 |
| MS.gene74137.t1 | MTR_4g092920 | 46.531 | 245 | 124 | 5 | 2 | 244 | 354 | 593 | 6.28e-61 | 206 |
| MS.gene74137.t1 | MTR_2g005690 | 44.715 | 246 | 129 | 5 | 2 | 245 | 373 | 613 | 1.86e-59 | 202 |
| MS.gene74137.t1 | MTR_6g034005 | 38.535 | 314 | 163 | 8 | 4 | 300 | 236 | 536 | 1.23e-53 | 186 |
| MS.gene74137.t1 | MTR_1g116250 | 42.379 | 269 | 113 | 5 | 34 | 300 | 60 | 288 | 1.82e-53 | 177 |
| MS.gene74137.t1 | MTR_7g074540 | 46.000 | 200 | 107 | 1 | 103 | 301 | 1 | 200 | 2.11e-51 | 169 |
| MS.gene74137.t1 | MTR_5g005870 | 40.984 | 244 | 138 | 5 | 4 | 244 | 88 | 328 | 8.46e-51 | 173 |
| MS.gene74137.t1 | MTR_6g033855 | 53.103 | 145 | 67 | 1 | 4 | 148 | 156 | 299 | 3.32e-46 | 159 |
| MS.gene74137.t1 | MTR_4g088515 | 74.757 | 103 | 26 | 0 | 19 | 121 | 515 | 617 | 5.15e-45 | 162 |
| MS.gene74137.t1 | MTR_7g451390 | 45.556 | 180 | 96 | 2 | 123 | 300 | 10 | 189 | 1.07e-43 | 148 |
| MS.gene74137.t1 | MTR_6g033810 | 59.000 | 100 | 40 | 1 | 4 | 102 | 192 | 291 | 3.36e-33 | 124 |
| MS.gene74137.t1 | MTR_1g068900 | 45.270 | 148 | 79 | 1 | 113 | 258 | 23 | 170 | 1.32e-31 | 116 |
| MS.gene74137.t1 | MTR_4g115780 | 40.212 | 189 | 111 | 2 | 2 | 188 | 462 | 650 | 1.08e-30 | 122 |
| MS.gene74137.t1 | MTR_2g040160 | 68.966 | 87 | 26 | 1 | 1 | 86 | 1411 | 1497 | 4.91e-30 | 120 |
| MS.gene74137.t1 | MTR_2g040260 | 67.816 | 87 | 27 | 1 | 1 | 86 | 1340 | 1426 | 2.32e-29 | 118 |
| MS.gene74137.t1 | MTR_2g040220 | 77.465 | 71 | 16 | 0 | 1 | 71 | 1324 | 1394 | 5.03e-29 | 117 |
| MS.gene74137.t1 | MTR_2g040220 | 50.526 | 95 | 38 | 3 | 135 | 220 | 1399 | 1493 | 5.47e-19 | 88.2 |
| MS.gene74137.t1 | MTR_2g031670 | 86.207 | 58 | 8 | 0 | 38 | 95 | 283 | 340 | 2.37e-27 | 109 |
| MS.gene74137.t1 | MTR_2g040350 | 66.667 | 87 | 28 | 1 | 1 | 86 | 1344 | 1430 | 2.63e-27 | 112 |
| MS.gene74137.t1 | MTR_2g040350 | 66.667 | 87 | 28 | 1 | 1 | 86 | 1126 | 1212 | 3.13e-27 | 112 |
| MS.gene74137.t1 | MTR_2g040370 | 65.854 | 82 | 28 | 0 | 1 | 82 | 1331 | 1412 | 4.21e-27 | 112 |
| MS.gene74137.t1 | MTR_2g040370 | 65.432 | 81 | 28 | 0 | 1 | 81 | 1716 | 1796 | 5.88e-20 | 91.3 |
| MS.gene74137.t1 | MTR_2g040370 | 65.854 | 82 | 28 | 0 | 1 | 82 | 1331 | 1412 | 4.24e-27 | 112 |
| MS.gene74137.t1 | MTR_2g040370 | 68.000 | 75 | 24 | 0 | 1 | 75 | 1716 | 1790 | 4.27e-19 | 88.6 |
| MS.gene74137.t1 | MTR_7g026180 | 34.562 | 217 | 111 | 4 | 88 | 302 | 144 | 331 | 4.91e-26 | 105 |
| MS.gene74137.t1 | MTR_4g107920 | 36.898 | 187 | 112 | 5 | 2 | 184 | 325 | 509 | 6.77e-26 | 107 |
| MS.gene74137.t1 | MTR_5g006180 | 36.264 | 182 | 98 | 4 | 2 | 179 | 340 | 507 | 2.78e-25 | 106 |
| MS.gene74137.t1 | MTR_2g040355 | 36.170 | 188 | 73 | 4 | 45 | 231 | 87 | 228 | 4.63e-23 | 97.1 |
| MS.gene74137.t1 | MTR_2g032160 | 72.131 | 61 | 17 | 0 | 1 | 61 | 315 | 375 | 3.56e-22 | 96.7 |
| MS.gene74137.t1 | MTR_2g032160 | 39.394 | 99 | 59 | 1 | 204 | 301 | 365 | 463 | 4.36e-12 | 67.0 |
| MS.gene74137.t1 | MTR_5g090110 | 35.542 | 166 | 96 | 4 | 3 | 164 | 324 | 482 | 1.07e-20 | 92.8 |
| MS.gene74137.t1 | MTR_7g026190 | 59.701 | 67 | 18 | 2 | 1 | 67 | 36 | 93 | 4.28e-17 | 79.0 |
| MS.gene74137.t1 | MTR_7g026190 | 50.000 | 74 | 37 | 0 | 183 | 256 | 94 | 167 | 1.35e-15 | 74.7 |
| MS.gene74137.t1 | MTR_7g026095 | 36.496 | 137 | 75 | 3 | 130 | 254 | 207 | 343 | 2.56e-16 | 79.0 |
| MS.gene74137.t1 | MTR_2g017710 | 36.719 | 128 | 56 | 3 | 39 | 164 | 87 | 191 | 2.90e-14 | 72.0 |
| MS.gene74137.t1 | MTR_4g115860 | 40.860 | 93 | 53 | 2 | 83 | 173 | 29 | 121 | 1.15e-13 | 67.0 |
| MS.gene74137.t1 | MTR_3g081170 | 24.873 | 197 | 126 | 6 | 2 | 179 | 347 | 540 | 3.31e-12 | 67.8 |
| MS.gene74137.t1 | MTR_6g478100 | 41.053 | 95 | 55 | 1 | 200 | 293 | 208 | 302 | 3.40e-12 | 66.6 |
| MS.gene74137.t1 | MTR_6g033815 | 50.877 | 57 | 28 | 0 | 209 | 265 | 1 | 57 | 2.96e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74137.t1 | AT3G12580 | 59.283 | 307 | 122 | 3 | 1 | 304 | 313 | 619 | 2.37e-118 | 355 |
| MS.gene74137.t1 | AT5G02500 | 57.566 | 304 | 126 | 3 | 1 | 301 | 313 | 616 | 2.09e-113 | 342 |
| MS.gene74137.t1 | AT1G16030 | 57.655 | 307 | 127 | 3 | 1 | 304 | 312 | 618 | 1.17e-112 | 340 |
| MS.gene74137.t1 | AT5G02490 | 56.250 | 304 | 130 | 3 | 1 | 301 | 313 | 616 | 1.97e-111 | 337 |
| MS.gene74137.t1 | AT3G09440 | 56.908 | 304 | 128 | 3 | 1 | 301 | 313 | 616 | 2.15e-110 | 334 |
| MS.gene74137.t1 | AT3G09440 | 56.908 | 304 | 128 | 3 | 1 | 301 | 313 | 616 | 2.15e-110 | 334 |
| MS.gene74137.t1 | AT3G09440 | 56.908 | 304 | 128 | 3 | 1 | 301 | 313 | 616 | 2.15e-110 | 334 |
| MS.gene74137.t1 | AT3G09440 | 56.908 | 304 | 128 | 3 | 1 | 301 | 313 | 616 | 2.15e-110 | 334 |
| MS.gene74137.t1 | AT1G56410 | 54.723 | 307 | 134 | 4 | 1 | 304 | 313 | 617 | 1.63e-106 | 323 |
| MS.gene74137.t1 | AT5G42020 | 50.000 | 296 | 144 | 4 | 2 | 293 | 339 | 634 | 1.41e-88 | 278 |
| MS.gene74137.t1 | AT5G42020 | 50.000 | 296 | 144 | 4 | 2 | 293 | 344 | 639 | 1.99e-88 | 278 |
| MS.gene74137.t1 | AT5G28540 | 49.662 | 296 | 145 | 4 | 2 | 293 | 339 | 634 | 3.52e-88 | 277 |
| MS.gene74137.t1 | AT1G09080 | 45.902 | 305 | 161 | 4 | 1 | 301 | 342 | 646 | 1.06e-71 | 234 |
| MS.gene74137.t1 | AT1G09080 | 45.902 | 305 | 161 | 4 | 1 | 301 | 352 | 656 | 1.33e-71 | 234 |
| MS.gene74137.t1 | AT5G49910 | 41.424 | 309 | 171 | 7 | 2 | 307 | 382 | 683 | 3.12e-61 | 207 |
| MS.gene74137.t1 | AT5G09590 | 45.492 | 244 | 126 | 5 | 3 | 244 | 361 | 599 | 1.95e-60 | 204 |
| MS.gene74137.t1 | AT4G24280 | 41.391 | 302 | 167 | 7 | 2 | 300 | 382 | 676 | 2.79e-60 | 204 |
| MS.gene74137.t1 | AT4G37910 | 47.131 | 244 | 122 | 5 | 3 | 244 | 356 | 594 | 1.03e-55 | 192 |
| MS.gene74137.t1 | AT4G37910 | 47.131 | 244 | 122 | 5 | 3 | 244 | 356 | 594 | 1.03e-55 | 192 |
| MS.gene74137.t1 | AT5G42020 | 53.333 | 180 | 80 | 4 | 2 | 177 | 339 | 518 | 5.01e-51 | 178 |
| MS.gene74137.t1 | AT2G32120 | 38.660 | 194 | 115 | 3 | 3 | 192 | 331 | 524 | 9.10e-35 | 133 |
| MS.gene74137.t1 | AT2G32120 | 38.660 | 194 | 115 | 3 | 3 | 192 | 331 | 524 | 9.10e-35 | 133 |
| MS.gene74137.t1 | AT5G02500 | 80.000 | 75 | 15 | 0 | 1 | 75 | 313 | 387 | 1.02e-33 | 129 |
| MS.gene74137.t1 | AT5G02500 | 40.860 | 93 | 55 | 0 | 201 | 293 | 386 | 478 | 3.24e-14 | 73.6 |
| MS.gene74137.t1 | AT4G16660 | 24.873 | 197 | 126 | 5 | 2 | 179 | 346 | 539 | 4.33e-12 | 67.4 |
| MS.gene74137.t1 | AT4G16660 | 24.873 | 197 | 126 | 5 | 2 | 179 | 370 | 563 | 4.87e-12 | 67.0 |
Find 60 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACCCTCATAAACCATAAT+TGG | 0.150011 | 7.4:-75475072 | None:intergenic |
| TCCTTGTTGGTGGTTCTTCT+AGG | 0.229614 | 7.4:+75474764 | MS.gene74137:CDS |
| CCAATCTCCAGTCCCAATTA+TGG | 0.247318 | 7.4:+75475059 | MS.gene74137:CDS |
| CTGTCTTGTTGATGCTAAAA+TGG | 0.256497 | 7.4:+75474717 | MS.gene74137:CDS |
| ATTACAATAACTAATGAAAA+TGG | 0.292577 | 7.4:+75475252 | MS.gene74137:CDS |
| CCTTGAAATTCTCAGCTTCC+TGG | 0.297903 | 7.4:-75475308 | None:intergenic |
| TGTAATAACTGCCTCACTTT+AGG | 0.312891 | 7.4:-75474790 | None:intergenic |
| TAAAGTGAGGCAGTTATTAC+AGG | 0.333837 | 7.4:+75474792 | MS.gene74137:CDS |
| CCTGATGAGGCTGTTGCTTA+TGG | 0.340665 | 7.4:+75474853 | MS.gene74137:CDS |
| AGAAGATACCTTTGTGTTTG+TGG | 0.342991 | 7.4:+75475509 | MS.gene74137:CDS |
| GTCGATGATGTTGTCCTTGT+TGG | 0.366819 | 7.4:+75474751 | MS.gene74137:CDS |
| ACATCTCTCAGCACCATGTT+TGG | 0.373927 | 7.4:-75474922 | None:intergenic |
| TTGATCTTCTCTTTGTCTAC+AGG | 0.380282 | 7.4:-75475441 | None:intergenic |
| AGTTGAAGCAAGAAATGCTT+TGG | 0.381737 | 7.4:+75475356 | MS.gene74137:CDS |
| TGCTTATGGTGCAGCTGTCC+AGG | 0.386356 | 7.4:+75474867 | MS.gene74137:CDS |
| CCATAAGCAACAGCCTCATC+AGG | 0.392319 | 7.4:-75474853 | None:intergenic |
| CTCTGATGGGGAAAGTGATT+CGG | 0.421571 | 7.4:+75475593 | MS.gene74137:CDS |
| AGTCCCAATTATGGTTTATG+AGG | 0.426752 | 7.4:+75475068 | MS.gene74137:CDS |
| AGAGATTGAGAGAATGATCC+AGG | 0.439899 | 7.4:+75475290 | MS.gene74137:CDS |
| ACACAAAGGTATCTTCTTGT+TGG | 0.445959 | 7.4:-75475503 | None:intergenic |
| TAATCTTGTGTCTTCTTGAA+TGG | 0.450954 | 7.4:-75475018 | None:intergenic |
| CCATAATTGGGACTGGAGAT+TGG | 0.454108 | 7.4:-75475059 | None:intergenic |
| ATGAAAATGGAAGATTATCA+AGG | 0.459072 | 7.4:+75475265 | MS.gene74137:CDS |
| CTTCAATACCAAGTGACAAA+GGG | 0.478189 | 7.4:-75474948 | None:intergenic |
| TTATGAGTGTGGTGATTCCT+AGG | 0.478815 | 7.4:+75474983 | MS.gene74137:CDS |
| CACCCTCATAAACCATAATT+GGG | 0.479306 | 7.4:-75475071 | None:intergenic |
| TCAACAAAGGTTACTCTGAT+GGG | 0.479872 | 7.4:+75475580 | MS.gene74137:CDS |
| GATGTTACCCCTTTGTCACT+TGG | 0.489905 | 7.4:+75474940 | MS.gene74137:CDS |
| TACTTTGATATAGATGCTAA+TGG | 0.501593 | 7.4:+75475180 | MS.gene74137:CDS |
| CCAGGAAGCTGAGAATTTCA+AGG | 0.503257 | 7.4:+75475308 | MS.gene74137:CDS |
| GGAAGGCCACGGGGAGCAAG+AGG | 0.503757 | 7.4:-75475147 | None:intergenic |
| AATGGTACAGAAGTATTCCT+AGG | 0.513462 | 7.4:-75475000 | None:intergenic |
| ATCAACAAAGGTTACTCTGA+TGG | 0.515621 | 7.4:+75475579 | MS.gene74137:CDS |
| TACTGTGAGAGGAAGGCCAC+GGG | 0.519886 | 7.4:-75475157 | None:intergenic |
| GATGATGTTGTCCTTGTTGG+TGG | 0.537032 | 7.4:+75474754 | MS.gene74137:CDS |
| TCATAAACCATAATTGGGAC+TGG | 0.565327 | 7.4:-75475066 | None:intergenic |
| CACTAAGAATGTTCCAAACA+TGG | 0.566528 | 7.4:+75474909 | MS.gene74137:CDS |
| GCAAGTGAGAACAACTTGCT+TGG | 0.568418 | 7.4:+75475105 | MS.gene74137:CDS |
| CAAAGTATACTGTGAGAGGA+AGG | 0.569317 | 7.4:-75475164 | None:intergenic |
| ACTTCAATACCAAGTGACAA+AGG | 0.588106 | 7.4:-75474949 | None:intergenic |
| GTCCCAATTATGGTTTATGA+GGG | 0.588752 | 7.4:+75475069 | MS.gene74137:CDS |
| CTTCACTAAGCAAAACAGCC+TGG | 0.589833 | 7.4:-75474885 | None:intergenic |
| TGAAAATGGAAGATTATCAA+GGG | 0.589866 | 7.4:+75475266 | MS.gene74137:CDS |
| TCACTTGGTATTGAAGTGAA+CGG | 0.593429 | 7.4:+75474955 | MS.gene74137:CDS |
| ATATCAAAGTATACTGTGAG+AGG | 0.593678 | 7.4:-75475168 | None:intergenic |
| CTTCTAGGATTCCTAAAGTG+AGG | 0.600875 | 7.4:+75474779 | MS.gene74137:CDS |
| TATATTAAACGTCTCTGCAG+TGG | 0.608686 | 7.4:+75475203 | MS.gene74137:CDS |
| GATCAACTCAGCAATGACCA+AGG | 0.625695 | 7.4:+75475458 | MS.gene74137:CDS |
| CAACAAAGGTTACTCTGATG+GGG | 0.627281 | 7.4:+75475581 | MS.gene74137:CDS |
| CATAAGCAACAGCCTCATCA+GGG | 0.634330 | 7.4:-75474852 | None:intergenic |
| CAAGAGCATTAACCCTGATG+AGG | 0.639574 | 7.4:+75474840 | MS.gene74137:CDS |
| GCTATGAACAAGATCAACAA+AGG | 0.640616 | 7.4:+75475567 | MS.gene74137:CDS |
| TTCAATACCAAGTGACAAAG+GGG | 0.642789 | 7.4:-75474947 | None:intergenic |
| AAAGTTCCTCTTGCTCCCCG+TGG | 0.642810 | 7.4:+75475141 | MS.gene74137:CDS |
| ATACTGTGAGAGGAAGGCCA+CGG | 0.644258 | 7.4:-75475158 | None:intergenic |
| TCCTAGAAGAACCACCAACA+AGG | 0.646014 | 7.4:-75474765 | None:intergenic |
| ATCAACTCAGCAATGACCAA+GGG | 0.650698 | 7.4:+75475459 | MS.gene74137:CDS |
| GAACGGAGATATTATGAGTG+TGG | 0.661270 | 7.4:+75474972 | MS.gene74137:CDS |
| TCTGCAGTGGAAGAAACCAG+TGG | 0.733951 | 7.4:+75475216 | MS.gene74137:CDS |
| ACTGTGAGAGGAAGGCCACG+GGG | 0.773300 | 7.4:-75475156 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTACAATAACTAATGAAAA+TGG | + | chr7.4:75475252-75475271 | MS.gene74137:CDS | 15.0% |
| ! | ATGAAAATGGAAGATTATCA+AGG | + | chr7.4:75475265-75475284 | MS.gene74137:CDS | 25.0% |
| ! | TACTTTGATATAGATGCTAA+TGG | + | chr7.4:75475180-75475199 | MS.gene74137:CDS | 25.0% |
| ! | TGAAAATGGAAGATTATCAA+GGG | + | chr7.4:75475266-75475285 | MS.gene74137:CDS | 25.0% |
| !!! | AATTTCATTTTTATTGCCAC+TGG | - | chr7.4:75475235-75475254 | None:intergenic | 25.0% |
| !!! | TATTACAGGAGTTTTTCAAA+GGG | + | chr7.4:75474806-75474825 | MS.gene74137:CDS | 25.0% |
| !!! | TTATTACAGGAGTTTTTCAA+AGG | + | chr7.4:75474805-75474824 | MS.gene74137:CDS | 25.0% |
| ATATCAAAGTATACTGTGAG+AGG | - | chr7.4:75475171-75475190 | None:intergenic | 30.0% | |
| TAATCTTGTGTCTTCTTGAA+TGG | - | chr7.4:75475021-75475040 | None:intergenic | 30.0% | |
| ! | TGTACATACACTTTTCAAAG+AGG | - | chr7.4:75474687-75474706 | None:intergenic | 30.0% |
| !! | ATCAATCAAACTTTTTCCCT+TGG | - | chr7.4:75475478-75475497 | None:intergenic | 30.0% |
| ACTTCAATACCAAGTGACAA+AGG | - | chr7.4:75474952-75474971 | None:intergenic | 35.0% | |
| AGAAGATACCTTTGTGTTTG+TGG | + | chr7.4:75475509-75475528 | MS.gene74137:CDS | 35.0% | |
| AGTCCCAATTATGGTTTATG+AGG | + | chr7.4:75475068-75475087 | MS.gene74137:CDS | 35.0% | |
| AGTTGAAGCAAGAAATGCTT+TGG | + | chr7.4:75475356-75475375 | MS.gene74137:CDS | 35.0% | |
| ATCAACAAAGGTTACTCTGA+TGG | + | chr7.4:75475579-75475598 | MS.gene74137:CDS | 35.0% | |
| CAAAAAGTCCACAAACACAA+AGG | - | chr7.4:75475520-75475539 | None:intergenic | 35.0% | |
| CACCCTCATAAACCATAATT+GGG | - | chr7.4:75475074-75475093 | None:intergenic | 35.0% | |
| CACTAAGAATGTTCCAAACA+TGG | + | chr7.4:75474909-75474928 | MS.gene74137:CDS | 35.0% | |
| CTTCAATACCAAGTGACAAA+GGG | - | chr7.4:75474951-75474970 | None:intergenic | 35.0% | |
| GCTATGAACAAGATCAACAA+AGG | + | chr7.4:75475567-75475586 | MS.gene74137:CDS | 35.0% | |
| GTCCCAATTATGGTTTATGA+GGG | + | chr7.4:75475069-75475088 | MS.gene74137:CDS | 35.0% | |
| TAAAGTGAGGCAGTTATTAC+AGG | + | chr7.4:75474792-75474811 | MS.gene74137:CDS | 35.0% | |
| TATATTAAACGTCTCTGCAG+TGG | + | chr7.4:75475203-75475222 | MS.gene74137:CDS | 35.0% | |
| TCAACAAAGGTTACTCTGAT+GGG | + | chr7.4:75475580-75475599 | MS.gene74137:CDS | 35.0% | |
| TCACCCTCATAAACCATAAT+TGG | - | chr7.4:75475075-75475094 | None:intergenic | 35.0% | |
| TCATAAACCATAATTGGGAC+TGG | - | chr7.4:75475069-75475088 | None:intergenic | 35.0% | |
| TGTAATAACTGCCTCACTTT+AGG | - | chr7.4:75474793-75474812 | None:intergenic | 35.0% | |
| TTCAATACCAAGTGACAAAG+GGG | - | chr7.4:75474950-75474969 | None:intergenic | 35.0% | |
| TTGATCTTCTCTTTGTCTAC+AGG | - | chr7.4:75475444-75475463 | None:intergenic | 35.0% | |
| ! | CTGTCTTGTTGATGCTAAAA+TGG | + | chr7.4:75474717-75474736 | MS.gene74137:CDS | 35.0% |
| ! | TCACTTGGTATTGAAGTGAA+CGG | + | chr7.4:75474955-75474974 | MS.gene74137:CDS | 35.0% |
| ! | TGTGTTTGTGGACTTTTTGA+AGG | + | chr7.4:75475521-75475540 | MS.gene74137:CDS | 35.0% |
| !! | AATGGTACAGAAGTATTCCT+AGG | - | chr7.4:75475003-75475022 | None:intergenic | 35.0% |
| !! | ACACAAAGGTATCTTCTTGT+TGG | - | chr7.4:75475506-75475525 | None:intergenic | 35.0% |
| AGAGATTGAGAGAATGATCC+AGG | + | chr7.4:75475290-75475309 | MS.gene74137:CDS | 40.0% | |
| ATCAACTCAGCAATGACCAA+GGG | + | chr7.4:75475459-75475478 | MS.gene74137:CDS | 40.0% | |
| CAAAGTATACTGTGAGAGGA+AGG | - | chr7.4:75475167-75475186 | None:intergenic | 40.0% | |
| CAACAAAGGTTACTCTGATG+GGG | + | chr7.4:75475581-75475600 | MS.gene74137:CDS | 40.0% | |
| GAACGGAGATATTATGAGTG+TGG | + | chr7.4:75474972-75474991 | MS.gene74137:CDS | 40.0% | |
| TTATGAGTGTGGTGATTCCT+AGG | + | chr7.4:75474983-75475002 | MS.gene74137:CDS | 40.0% | |
| !! | ACAGGAGTTTTTCAAAGGGA+AGG | + | chr7.4:75474810-75474829 | MS.gene74137:CDS | 40.0% |
| !! | CTTCTAGGATTCCTAAAGTG+AGG | + | chr7.4:75474779-75474798 | MS.gene74137:CDS | 40.0% |
| ACATCTCTCAGCACCATGTT+TGG | - | chr7.4:75474925-75474944 | None:intergenic | 45.0% | |
| CATAAGCAACAGCCTCATCA+GGG | - | chr7.4:75474855-75474874 | None:intergenic | 45.0% | |
| CCAATCTCCAGTCCCAATTA+TGG | + | chr7.4:75475059-75475078 | MS.gene74137:CDS | 45.0% | |
| CCAGGAAGCTGAGAATTTCA+AGG | + | chr7.4:75475308-75475327 | MS.gene74137:CDS | 45.0% | |
| CCATAATTGGGACTGGAGAT+TGG | - | chr7.4:75475062-75475081 | None:intergenic | 45.0% | |
| CCTTGAAATTCTCAGCTTCC+TGG | - | chr7.4:75475311-75475330 | None:intergenic | 45.0% | |
| CTCTGATGGGGAAAGTGATT+CGG | + | chr7.4:75475593-75475612 | MS.gene74137:CDS | 45.0% | |
| CTTCACTAAGCAAAACAGCC+TGG | - | chr7.4:75474888-75474907 | None:intergenic | 45.0% | |
| GATCAACTCAGCAATGACCA+AGG | + | chr7.4:75475458-75475477 | MS.gene74137:CDS | 45.0% | |
| GATGATGTTGTCCTTGTTGG+TGG | + | chr7.4:75474754-75474773 | MS.gene74137:CDS | 45.0% | |
| GATGTTACCCCTTTGTCACT+TGG | + | chr7.4:75474940-75474959 | MS.gene74137:CDS | 45.0% | |
| GTCGATGATGTTGTCCTTGT+TGG | + | chr7.4:75474751-75474770 | MS.gene74137:CDS | 45.0% | |
| TCCTAGAAGAACCACCAACA+AGG | - | chr7.4:75474768-75474787 | None:intergenic | 45.0% | |
| ! | GCAAGTGAGAACAACTTGCT+TGG | + | chr7.4:75475105-75475124 | MS.gene74137:CDS | 45.0% |
| !! | CAAGAGCATTAACCCTGATG+AGG | + | chr7.4:75474840-75474859 | MS.gene74137:CDS | 45.0% |
| !! | TCCTTGTTGGTGGTTCTTCT+AGG | + | chr7.4:75474764-75474783 | MS.gene74137:CDS | 45.0% |
| ATACTGTGAGAGGAAGGCCA+CGG | - | chr7.4:75475161-75475180 | None:intergenic | 50.0% | |
| CCATAAGCAACAGCCTCATC+AGG | - | chr7.4:75474856-75474875 | None:intergenic | 50.0% | |
| TCTGCAGTGGAAGAAACCAG+TGG | + | chr7.4:75475216-75475235 | MS.gene74137:CDS | 50.0% | |
| ! | CCTGATGAGGCTGTTGCTTA+TGG | + | chr7.4:75474853-75474872 | MS.gene74137:CDS | 50.0% |
| AAAGTTCCTCTTGCTCCCCG+TGG | + | chr7.4:75475141-75475160 | MS.gene74137:CDS | 55.0% | |
| TACTGTGAGAGGAAGGCCAC+GGG | - | chr7.4:75475160-75475179 | None:intergenic | 55.0% | |
| TGCTTATGGTGCAGCTGTCC+AGG | + | chr7.4:75474867-75474886 | MS.gene74137:CDS | 55.0% | |
| ACTGTGAGAGGAAGGCCACG+GGG | - | chr7.4:75475159-75475178 | None:intergenic | 60.0% | |
| GGAAGGCCACGGGGAGCAAG+AGG | - | chr7.4:75475150-75475169 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 75474679 | 75475623 | 75474679 | ID=MS.gene74137 |
| chr7.4 | mRNA | 75474679 | 75475623 | 75474679 | ID=MS.gene74137.t1;Parent=MS.gene74137 |
| chr7.4 | exon | 75474679 | 75475623 | 75474679 | ID=MS.gene74137.t1.exon1;Parent=MS.gene74137.t1 |
| chr7.4 | CDS | 75474679 | 75475623 | 75474679 | ID=cds.MS.gene74137.t1;Parent=MS.gene74137.t1 |
| Gene Sequence |
| Protein sequence |
