AlfalfaGEDB | MS.gene74244

M. sativa cultivar XinJiangDaYe / MS.gene74244

KEGG: -
GO: -
PFAM: PF07939.14

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74244.t1	XP_003592837.1	69.7	109	3	2	2	83	11	116	2.30E-31	144.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74244.t1	G7IB02	69.7	109	3	2	2	83	11	116	1.7e-31	144.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04915	MS.gene74244	0.806092	9.73E-50	-1.69E-46
MS.gene04917	MS.gene74244	0.802569	5.27E-49	-1.69E-46
MS.gene050361	MS.gene74244	0.806966	6.36E-50	-1.69E-46
MS.gene050442	MS.gene74244	0.803139	4.02E-49	-1.69E-46
MS.gene050762	MS.gene74244	0.855556	5.83E-62	-1.69E-46
MS.gene050763	MS.gene74244	0.854911	8.98E-62	-1.69E-46
MS.gene050826	MS.gene74244	0.802578	5.25E-49	-1.69E-46
MS.gene054379	MS.gene74244	0.855505	6.03E-62	-1.69E-46
MS.gene054930	MS.gene74244	0.811588	6.49E-51	-1.69E-46
MS.gene056480	MS.gene74244	0.821578	3.75E-53	-1.69E-46
MS.gene058399	MS.gene74244	0.809807	1.58E-50	-1.69E-46
MS.gene059713	MS.gene74244	0.812383	4.36E-51	-1.69E-46
MS.gene060084	MS.gene74244	0.822798	1.95E-53	-1.69E-46
MS.gene06052	MS.gene74244	0.817765	2.78E-52	-1.69E-46
MS.gene060683	MS.gene74244	0.811158	8.05E-51	-1.69E-46
MS.gene060685	MS.gene74244	0.805641	1.21E-49	-1.69E-46
MS.gene060802	MS.gene74244	0.803177	3.95E-49	-1.69E-46
MS.gene060803	MS.gene74244	0.818328	2.08E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74244.t1	MTR_1g116590	69.725	109	3	2	2	83	11	116	5.65e-44	138

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74244.t1	AT2G31560	45.522	134	21	1	2	83	69	202	1.52e-29	104
MS.gene74244.t1	AT2G31560	45.522	134	21	1	2	83	69	202	1.52e-29	104
MS.gene74244.t1	AT2G31560	45.522	134	21	1	2	83	69	202	1.52e-29	104
MS.gene74244.t1	AT1G05870	43.357	143	20	2	2	83	47	189	1.05e-28	101
MS.gene74244.t1	AT1G05870	43.357	143	20	2	2	83	47	189	1.05e-28	101
MS.gene74244.t1	AT1G05870	43.357	143	20	2	2	83	47	189	1.05e-28	101
MS.gene74244.t1	AT1G05870	43.357	143	20	2	2	83	47	189	1.05e-28	101
MS.gene74244.t1	AT1G05870	43.357	143	20	2	2	83	47	189	1.05e-28	101
MS.gene74244.t1	AT1G05870	43.357	143	20	2	2	83	47	189	1.05e-28	101
MS.gene74244.t1	AT1G05870	43.357	143	20	2	2	83	47	189	1.05e-28	101
MS.gene74244.t1	AT1G05870	43.357	143	20	2	2	83	47	189	1.05e-28	101
MS.gene74244.t1	AT1G05870	43.357	143	20	2	2	83	47	189	1.05e-28	101
MS.gene74244.t1	AT1G05870	43.357	143	20	2	2	83	47	189	1.05e-28	101
MS.gene74244.t1	AT1G05870	43.357	143	20	2	2	83	47	189	1.05e-28	101
MS.gene74244.t1	AT1G05870	43.357	143	20	2	2	83	47	189	1.05e-28	101
MS.gene74244.t1	AT2G43340	45.238	126	15	2	12	83	64	189	7.74e-26	94.4
MS.gene74244.t1	AT3G22690	44.444	81	8	1	31	74	843	923	7.32e-13	62.4

Find 19 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGATGTTTGGATCTTGGTTT+TGG	0.184629	1.1:-82145757	MS.gene74244:CDS
TTAAAGCAAGATTGAAATAT+TGG	0.250359	1.1:-82145599	MS.gene74244:CDS
AATCTTGCTTTAACATCTTC+AGG	0.331635	1.1:+82145609	None:intergenic
TGATGAGTTGAAGGGATGTT+TGG	0.374718	1.1:-82145770	MS.gene74244:CDS
TAAAGCAAGATTGAAATATT+GGG	0.375422	1.1:-82145598	MS.gene74244:CDS
TTGAAGGGATGTTTGGATCT+TGG	0.389102	1.1:-82145763	MS.gene74244:CDS
TAGTTAGCTGCAGAGTTTAA+CGG	0.390779	1.1:+82145557	None:intergenic
GGGAGGGTGTTACATAGTTC+AGG	0.412792	1.1:+82145715	None:intergenic
CATCAAGATCCTCATCTGTT+AGG	0.424926	1.1:+82145788	None:intergenic
TGAGGATCTTGATGAGTTGA+AGG	0.481670	1.1:-82145779	MS.gene74244:CDS
TAACAAAGGAGTAGTGTTGT+TGG	0.483852	1.1:-82145857	MS.gene74244:CDS
ATAATGAGGAGGATCTTTGT+AGG	0.495986	1.1:-82145819	MS.gene74244:CDS
AAGGAGTAGTGTTGTTGGAA+GGG	0.511274	1.1:-82145852	MS.gene74244:CDS
AAAGGAGTAGTGTTGTTGGA+AGG	0.535317	1.1:-82145853	MS.gene74244:CDS
AGGGTATGTTGATGATAATG+AGG	0.584846	1.1:-82145833	MS.gene74244:CDS
GACGAAAAGCCTAACAGATG+AGG	0.616832	1.1:-82145797	MS.gene74244:CDS
AGTAAGAATAACAATAACAA+AGG	0.625105	1.1:-82145871	MS.gene74244:CDS
GAGGATCTTGATGAGTTGAA+GGG	0.630888	1.1:-82145778	MS.gene74244:CDS
GTATGTTGATGATAATGAGG+AGG	0.675262	1.1:-82145830	MS.gene74244:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTAAGAATAACAATAACAA+AGG	-	chr1.1:82145566-82145585	MS.gene74244:CDS	20.0%
!!	TAAAGCAAGATTGAAATATT+GGG	-	chr1.1:82145839-82145858	MS.gene74244:CDS	20.0%
!!	TTAAAGCAAGATTGAAATAT+TGG	-	chr1.1:82145838-82145857	MS.gene74244:CDS	20.0%
	AATCTTGCTTTAACATCTTC+AGG	+	chr1.1:82145831-82145850	None:intergenic	30.0%
	AATTGGAAGATATCAAGTCC+AGG	-	chr1.1:82145798-82145817	MS.gene74244:CDS	35.0%
	ATAATGAGGAGGATCTTTGT+AGG	-	chr1.1:82145618-82145637	MS.gene74244:CDS	35.0%
	TAACAAAGGAGTAGTGTTGT+TGG	-	chr1.1:82145580-82145599	MS.gene74244:CDS	35.0%
	TCCAATTGGCAAAATGAATC+AGG	+	chr1.1:82145785-82145804	None:intergenic	35.0%
	TGGACTTGATATCTTCCAAT+TGG	+	chr1.1:82145799-82145818	None:intergenic	35.0%
!	TCCTGATTCATTTTGCCAAT+TGG	-	chr1.1:82145781-82145800	MS.gene74244:CDS	35.0%
!!	AATAACACAACTCTAGAGCT+GGG	+	chr1.1:82145745-82145764	None:intergenic	35.0%
!!	AGGGTATGTTGATGATAATG+AGG	-	chr1.1:82145604-82145623	MS.gene74244:CDS	35.0%
!!	GATGTTTGGATCTTGGTTTT+GGG	-	chr1.1:82145681-82145700	MS.gene74244:intron	35.0%
!!	GTATGTTGATGATAATGAGG+AGG	-	chr1.1:82145607-82145626	MS.gene74244:CDS	35.0%
	CATCAAGATCCTCATCTGTT+AGG	+	chr1.1:82145652-82145671	None:intergenic	40.0%
	TTGAAGGGATGTTTGGATCT+TGG	-	chr1.1:82145674-82145693	MS.gene74244:intron	40.0%
!	AAAGGAGTAGTGTTGTTGGA+AGG	-	chr1.1:82145584-82145603	MS.gene74244:CDS	40.0%
!	AAGGAGTAGTGTTGTTGGAA+GGG	-	chr1.1:82145585-82145604	MS.gene74244:CDS	40.0%
!	GAGGATCTTGATGAGTTGAA+GGG	-	chr1.1:82145659-82145678	MS.gene74244:intron	40.0%
!	GGATGTTTGGATCTTGGTTT+TGG	-	chr1.1:82145680-82145699	MS.gene74244:intron	40.0%
!	TGAGGATCTTGATGAGTTGA+AGG	-	chr1.1:82145658-82145677	MS.gene74244:intron	40.0%
!	TGATGAGTTGAAGGGATGTT+TGG	-	chr1.1:82145667-82145686	MS.gene74244:intron	40.0%
!!	GAATAACACAACTCTAGAGC+TGG	+	chr1.1:82145746-82145765	None:intergenic	40.0%
	ACATCTTCAGGATGATCACC+TGG	+	chr1.1:82145819-82145838	None:intergenic	45.0%
	GACGAAAAGCCTAACAGATG+AGG	-	chr1.1:82145640-82145659	MS.gene74244:intron	45.0%
!!	AACACAACTCTAGAGCTGGG+AGG	+	chr1.1:82145742-82145761	None:intergenic	50.0%
!!	ACACAACTCTAGAGCTGGGA+GGG	+	chr1.1:82145741-82145760	None:intergenic	50.0%
!!	GGGAGGGTGTTACATAGTTC+AGG	+	chr1.1:82145725-82145744	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	82145560	82145899	82145560	ID=MS.gene74244
chr1.1	mRNA	82145560	82145899	82145560	ID=MS.gene74244.t1;Parent=MS.gene74244
chr1.1	exon	82145728	82145899	82145728	ID=MS.gene74244.t1.exon1;Parent=MS.gene74244.t1
chr1.1	CDS	82145728	82145899	82145728	ID=cds.MS.gene74244.t1;Parent=MS.gene74244.t1
chr1.1	exon	82145560	82145639	82145560	ID=MS.gene74244.t1.exon2;Parent=MS.gene74244.t1
chr1.1	CDS	82145560	82145639	82145560	ID=cds.MS.gene74244.t1;Parent=MS.gene74244.t1

Gene Sequence	>MS.gene74244 ATGAAGAGTAAGAATAACAATAACAAAGGAGTAGTGTTGTTGGAAGGGTATGTTGATGATAATGAGGAGGATCTTTGTAGGACGAAAAGCCTAACAGATGAGGATCTTGATGAGTTGAAGGGATGTTTGGATCTTGGTTTTGGGTTCAGTTATGATGAGATTCCTGAACTATGTAACACCCTCCCAGCTCTAGAGTTGTGTTATTCAATGTCAAACACTTCTCCTGATTCATTTTGCCAATTGGAAGATATCAAGTCCAGGTGATCATCCTGAAGATGTTAAAGCAAGATTGAAATATTGGGCACAAGCTGTTGCGTGTACCGTTAAACTCTGCAGCTAA
Protein sequence	>MS.gene74244.t1 MKSKNNNNKGVVLLEGYVDDNEEDLCRTKSLTDEDLDELKGCLDLGFGFSYDEIPELCDHPEDVKARLKYWAQAVACTVKLCS