AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74966

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74966.t1 MTR_1g112210 95.026 382 15 1 1 378 1 382 0.0 764
MS.gene74966.t1 MTR_1g112210 93.056 360 12 1 19 378 23 369 0.0 702
MS.gene74966.t1 MTR_1g050448 73.351 379 96 3 1 378 1 375 0.0 601
MS.gene74966.t1 MTR_3g464570 42.515 334 174 5 55 374 43 372 5.11e-99 299
MS.gene74966.t1 MTR_5g071350 40.525 343 180 5 53 374 56 395 1.30e-98 298
MS.gene74966.t1 MTR_2g015720 42.598 331 169 6 55 378 42 358 1.31e-95 290
MS.gene74966.t1 MTR_4g016940 42.075 347 177 8 53 378 84 427 2.99e-92 283
MS.gene74966.t1 MTR_3g049330 42.216 334 169 7 55 378 31 350 6.83e-92 280
MS.gene74966.t1 MTR_2g091175 39.759 332 179 6 54 378 49 366 8.75e-92 280
MS.gene74966.t1 MTR_7g093450 39.211 380 207 8 19 378 212 587 7.19e-90 282
MS.gene74966.t1 MTR_2g073500 42.687 335 167 8 53 378 27 345 4.75e-89 272
MS.gene74966.t1 MTR_0136s0010 41.499 347 183 7 50 378 165 509 2.35e-88 276
MS.gene74966.t1 MTR_2g073510 39.640 333 180 6 51 374 29 349 9.46e-88 269
MS.gene74966.t1 MTR_5g034810 40.719 334 176 7 53 378 33 352 2.56e-86 266
MS.gene74966.t1 MTR_4g079700 38.024 334 180 8 55 378 47 363 3.99e-85 263
MS.gene74966.t1 MTR_5g033800 36.842 380 216 10 15 378 112 483 7.06e-85 266
MS.gene74966.t1 MTR_7g109550 37.015 335 188 5 55 374 137 463 1.12e-84 265
MS.gene74966.t1 MTR_7g068920 40.058 347 184 8 53 378 78 421 1.93e-84 263
MS.gene74966.t1 MTR_5g059400 37.662 385 215 8 17 378 133 515 3.50e-84 265
MS.gene74966.t1 MTR_1g116130 37.662 385 215 8 17 378 133 515 3.50e-84 265
MS.gene74966.t1 MTR_8g463790 39.884 346 179 10 55 378 71 409 9.25e-83 258
MS.gene74966.t1 MTR_3g070900 40.517 348 171 10 55 374 106 445 1.98e-82 259
MS.gene74966.t1 MTR_4g087730 37.500 376 205 10 12 378 8 362 2.24e-82 256
MS.gene74966.t1 MTR_4g088030 36.047 344 200 6 42 378 20 350 2.95e-82 255
MS.gene74966.t1 MTR_4g014440 35.550 391 222 11 10 378 25 407 8.63e-82 256
MS.gene74966.t1 MTR_4g126950 34.896 384 220 8 21 378 67 446 9.57e-81 254
MS.gene74966.t1 MTR_5g082880 40.730 356 166 12 53 374 163 507 1.03e-79 253
MS.gene74966.t1 MTR_3g021090 35.694 353 204 7 30 374 7 344 1.70e-79 250
MS.gene74966.t1 MTR_3g035830 35.775 355 205 7 30 376 7 346 2.64e-79 247
MS.gene74966.t1 MTR_1g111010 40.964 332 170 12 53 378 30 341 1.05e-78 246
MS.gene74966.t1 MTR_3g071650 35.969 392 222 10 14 378 41 430 4.49e-78 247
MS.gene74966.t1 MTR_4g121560 36.413 368 209 8 31 378 91 453 1.90e-77 246
MS.gene74966.t1 MTR_3g021060 34.758 351 196 7 34 376 36 361 2.16e-76 240
MS.gene74966.t1 MTR_5g083260 38.261 345 189 9 55 378 83 424 2.76e-76 242
MS.gene74966.t1 MTR_8g069790 35.988 339 200 7 52 374 175 512 6.86e-75 241
MS.gene74966.t1 MTR_3g464570 41.481 270 141 4 118 374 3 268 4.47e-74 231
MS.gene74966.t1 MTR_7g070060 35.159 347 203 9 54 378 63 409 6.14e-74 236
MS.gene74966.t1 MTR_4g014430 35.530 349 206 7 47 378 100 446 5.34e-73 234
MS.gene74966.t1 MTR_1g058380 33.747 403 233 10 1 378 1 394 2.18e-70 226
MS.gene74966.t1 MTR_7g094750 35.260 346 198 8 55 378 91 432 8.38e-67 218
MS.gene74966.t1 MTR_2g022560 34.054 370 208 9 32 376 77 435 1.44e-66 218
MS.gene74966.t1 MTR_3g090120 35.372 376 205 9 25 378 100 459 6.62e-63 208
MS.gene74966.t1 MTR_8g093530 33.431 341 200 6 53 378 88 416 4.11e-61 203
MS.gene74966.t1 MTR_5g033800 37.262 263 148 6 28 279 124 380 1.15e-57 192
MS.gene74966.t1 MTR_7g070060 37.021 235 139 5 54 279 63 297 9.12e-54 180
MS.gene74966.t1 MTR_7g070060 32.082 293 179 7 106 378 15 307 3.03e-51 174
MS.gene74966.t1 MTR_7g094750 34.454 238 143 3 55 279 91 328 4.16e-48 166
MS.gene74966.t1 MTR_6g061710 29.459 370 215 12 40 374 152 510 3.23e-44 160
MS.gene74966.t1 MTR_1g076690 29.888 358 199 14 55 374 223 566 4.39e-40 150
MS.gene74966.t1 MTR_5g069320 30.466 279 152 6 98 371 145 386 2.61e-39 144
MS.gene74966.t1 MTR_8g093870 28.732 355 207 15 53 376 131 470 3.79e-36 137
MS.gene74966.t1 MTR_8g027900 27.793 367 222 14 40 374 196 551 1.04e-35 137
MS.gene74966.t1 MTR_7g019120 29.745 353 208 12 51 374 75 416 2.64e-35 134
MS.gene74966.t1 MTR_7g033230 28.611 360 203 13 53 374 262 605 1.43e-34 134
MS.gene74966.t1 MTR_5g035090 27.322 366 213 13 46 374 74 423 1.45e-34 132
MS.gene74966.t1 MTR_8g099300 27.020 396 246 14 11 374 31 415 1.63e-34 132
MS.gene74966.t1 MTR_7g033230 28.611 360 203 13 53 374 332 675 3.07e-34 134
MS.gene74966.t1 MTR_4g092830 27.557 352 214 11 55 375 48 389 2.44e-33 129
MS.gene74966.t1 MTR_3g109840 28.169 355 204 13 55 374 73 411 2.48e-33 129
MS.gene74966.t1 MTR_8g104970 27.596 366 213 15 46 374 111 461 5.56e-33 129
MS.gene74966.t1 MTR_6g007777 27.901 362 207 15 53 378 114 457 8.55e-33 128
MS.gene74966.t1 MTR_6g016880 25.735 408 254 12 7 374 17 415 1.53e-32 127
MS.gene74966.t1 MTR_2g016060 29.050 358 192 14 59 374 59 396 4.89e-32 125
MS.gene74966.t1 MTR_5g035150 26.776 366 209 13 50 374 80 427 5.84e-32 125
MS.gene74966.t1 MTR_5g035080 27.731 357 206 14 55 374 75 416 1.00e-30 122
MS.gene74966.t1 MTR_5g035070 29.494 356 202 13 55 374 87 429 1.35e-30 121
MS.gene74966.t1 MTR_4g125060 27.371 369 216 16 45 376 116 469 2.79e-30 121
MS.gene74966.t1 MTR_4g125060 27.654 358 213 15 52 378 168 510 3.04e-30 121
MS.gene74966.t1 MTR_4g125060 27.654 358 213 15 52 378 197 539 4.27e-30 121
MS.gene74966.t1 MTR_6g016875 27.042 355 214 10 53 374 62 404 3.72e-29 117
MS.gene74966.t1 MTR_6g016240 27.251 411 235 16 8 374 22 412 2.66e-26 109
MS.gene74966.t1 MTR_7g019140 46.875 96 50 1 51 145 75 170 1.42e-23 96.7
MS.gene74966.t1 MTR_5g035120 39.216 102 61 1 44 144 64 165 8.82e-20 86.3
MS.gene74966.t1 MTR_3g021150 34.579 107 63 2 240 339 1 107 8.82e-17 76.3
MS.gene74966.t1 MTR_2g022580 35.593 118 64 3 115 220 3 120 2.21e-15 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74966.t1 AT3G54260 62.005 379 130 6 2 376 4 372 1.79e-173 488
MS.gene74966.t1 AT5G58600 40.828 338 183 6 55 378 66 400 2.12e-93 285
MS.gene74966.t1 AT2G31110 38.736 364 197 7 21 374 11 358 5.54e-92 280
MS.gene74966.t1 AT1G29050 41.390 331 174 5 55 378 61 378 8.98e-92 280
MS.gene74966.t1 AT2G34070 40.118 339 180 7 49 378 59 383 9.41e-90 275
MS.gene74966.t1 AT2G30900 40.841 333 174 8 53 374 41 361 2.17e-89 274
MS.gene74966.t1 AT3G62390 38.872 337 186 6 53 374 136 467 6.10e-86 268
MS.gene74966.t1 AT5G01360 38.329 347 191 8 53 378 34 378 8.29e-86 265
MS.gene74966.t1 AT5G06700 38.251 366 201 7 37 378 230 594 3.02e-85 271
MS.gene74966.t1 AT5G01360 38.329 347 191 8 53 378 90 434 3.62e-85 265
MS.gene74966.t1 AT2G30010 40.058 347 179 8 55 378 56 396 4.82e-85 264
MS.gene74966.t1 AT2G30010 40.058 347 179 8 55 378 99 439 8.24e-85 265
MS.gene74966.t1 AT2G42570 38.507 335 180 7 54 378 46 364 1.58e-83 259
MS.gene74966.t1 AT1G73140 39.017 346 186 8 53 378 65 405 1.59e-83 260
MS.gene74966.t1 AT1G78710 38.298 329 182 7 54 374 37 352 3.12e-83 258
MS.gene74966.t1 AT3G12060 39.368 348 189 8 50 378 191 535 4.52e-83 263
MS.gene74966.t1 AT3G55990 37.669 369 200 9 28 374 119 479 1.29e-81 258
MS.gene74966.t1 AT2G40150 38.636 352 180 9 54 374 70 416 8.47e-81 254
MS.gene74966.t1 AT5G20590 38.663 344 190 8 53 378 139 479 9.80e-81 255
MS.gene74966.t1 AT5G49340 37.853 354 198 9 45 378 90 441 6.85e-80 252
MS.gene74966.t1 AT1G60790 36.705 346 198 9 49 374 184 528 1.16e-79 254
MS.gene74966.t1 AT5G01620 37.391 345 194 8 53 378 108 449 3.53e-79 250
MS.gene74966.t1 AT5G01620 37.391 345 194 8 53 378 108 449 3.53e-79 250
MS.gene74966.t1 AT3G14850 37.576 330 183 7 55 374 34 350 3.57e-79 247
MS.gene74966.t1 AT5G01620 37.391 345 194 8 53 378 116 457 4.72e-79 250
MS.gene74966.t1 AT2G40160 37.176 347 195 9 55 378 77 423 7.66e-78 246
MS.gene74966.t1 AT2G40160 36.888 347 196 9 55 378 77 423 9.22e-78 246
MS.gene74966.t1 AT3G11030 37.609 343 195 7 55 378 106 448 2.42e-76 243
MS.gene74966.t1 AT2G38320 32.663 398 241 10 3 378 12 404 2.41e-75 239
MS.gene74966.t1 AT2G40320 34.343 396 235 9 8 378 27 422 7.96e-74 236
MS.gene74966.t1 AT1G48880 35.057 348 181 8 54 378 114 439 3.70e-67 219
MS.gene74966.t1 AT5G19160 34.400 375 208 9 33 376 75 442 1.02e-66 218
MS.gene74966.t1 AT2G31110 38.583 254 153 3 20 272 10 261 2.86e-66 211
MS.gene74966.t1 AT2G38320 34.268 321 191 7 78 378 1 321 1.76e-65 211
MS.gene74966.t1 AT5G58600 41.850 227 129 3 55 279 66 291 1.80e-65 210
MS.gene74966.t1 AT3G62390 41.176 238 129 3 53 279 136 373 4.16e-65 213
MS.gene74966.t1 AT3G06080 35.311 354 194 9 50 376 98 443 4.58e-65 214
MS.gene74966.t1 AT2G30900 42.609 230 127 5 53 279 41 268 1.77e-64 207
MS.gene74966.t1 AT2G34070 39.163 263 138 6 124 378 1 249 2.12e-62 201
MS.gene74966.t1 AT5G06230 34.278 353 198 8 53 378 19 364 5.90e-62 203
MS.gene74966.t1 AT5G06230 34.278 353 198 8 53 378 60 405 1.10e-61 204
MS.gene74966.t1 AT3G11570 31.818 396 231 11 8 378 38 419 2.25e-60 201
MS.gene74966.t1 AT2G40160 39.407 236 132 6 55 279 77 312 1.59e-56 187
MS.gene74966.t1 AT1G78710 31.549 355 176 10 30 378 3 296 1.39e-55 184
MS.gene74966.t1 AT5G20590 38.750 240 134 5 53 279 139 378 9.01e-54 182
MS.gene74966.t1 AT5G19160 37.037 243 140 4 50 279 94 336 2.43e-50 172
MS.gene74966.t1 AT3G14850 35.385 260 146 6 124 374 1 247 5.11e-50 169
MS.gene74966.t1 AT2G31110 40.367 218 105 6 166 374 9 210 8.34e-49 164
MS.gene74966.t1 AT3G06080 35.802 243 143 4 50 279 98 340 1.84e-48 167
MS.gene74966.t1 AT5G06230 34.553 246 142 4 53 279 60 305 4.18e-45 157
MS.gene74966.t1 AT1G70230 30.447 358 190 14 55 378 79 411 1.78e-40 148
MS.gene74966.t1 AT4G25360 29.096 354 211 12 54 374 174 520 5.94e-39 146
MS.gene74966.t1 AT4G25360 29.096 354 211 12 54 374 174 520 5.94e-39 146
MS.gene74966.t1 AT5G51640 29.178 377 222 13 31 374 120 484 6.26e-39 145
MS.gene74966.t1 AT1G01430 26.216 370 237 12 35 374 80 443 4.29e-38 142
MS.gene74966.t1 AT4G01080 28.693 352 211 12 51 374 87 426 5.67e-37 139
MS.gene74966.t1 AT5G64020 27.324 355 220 15 55 376 57 406 1.31e-36 137
MS.gene74966.t1 AT5G20680 28.205 390 221 16 26 378 90 457 2.14e-34 132
MS.gene74966.t1 AT3G28150 28.713 404 215 18 12 376 39 408 3.72e-34 131
MS.gene74966.t1 AT5G20680 28.205 390 221 16 26 378 184 551 5.35e-34 132
MS.gene74966.t1 AT5G20680 28.205 390 221 16 26 378 184 551 5.35e-34 132
MS.gene74966.t1 AT5G20680 28.205 390 221 16 26 378 184 551 5.35e-34 132
MS.gene74966.t1 AT5G20680 28.205 390 221 16 26 378 184 551 5.35e-34 132
MS.gene74966.t1 AT5G20680 28.205 390 221 16 26 378 166 533 5.59e-34 132
MS.gene74966.t1 AT5G15890 27.841 352 214 10 49 374 181 518 1.82e-33 130
MS.gene74966.t1 AT2G37720 27.298 359 211 16 53 378 141 482 2.99e-33 129
MS.gene74966.t1 AT5G15900 30.280 393 205 17 26 378 46 409 3.37e-33 128
MS.gene74966.t1 AT5G15900 30.280 393 205 17 26 378 65 428 4.10e-33 129
MS.gene74966.t1 AT4G11090 28.019 414 245 18 1 374 21 421 6.89e-32 125
MS.gene74966.t1 AT5G64470 26.225 347 220 10 55 375 55 391 9.97e-32 124
MS.gene74966.t1 AT5G64470 26.062 353 219 11 55 375 55 397 6.73e-31 122
MS.gene74966.t1 AT2G14530 26.741 359 214 10 55 378 62 406 1.16e-30 121
MS.gene74966.t1 AT4G23790 27.147 361 210 14 53 374 77 423 8.16e-30 119
MS.gene74966.t1 AT3G02440 28.286 350 209 11 55 374 135 472 2.23e-29 119
MS.gene74966.t1 AT3G02440 28.286 350 209 11 55 374 127 464 2.68e-29 118
MS.gene74966.t1 AT5G64470 26.423 246 162 7 55 283 55 298 3.10e-21 93.6
MS.gene74966.t1 AT5G64470 26.423 246 162 7 55 283 55 298 3.83e-21 93.6

Find 93 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCTGGTGTGCCTGATATT+TGG 0.189016 1.3:-76077743 MS.gene74966:CDS
GTGATTTCTCAGTTGGAAAA+TGG 0.208372 1.3:-76079159 MS.gene74966:CDS
AGACATCAATTGAGTTCTTC+TGG 0.226253 1.3:-76078523 MS.gene74966:CDS
ATTTCTTCCATGAATCTCTT+TGG 0.226552 1.3:+76079182 None:intergenic
GCTCATTGGTGGACTCATTC+TGG 0.278727 1.3:-76078383 MS.gene74966:CDS
TGATTAAATTGTAGGCAAAA+TGG 0.281278 1.3:-76078008 MS.gene74966:intron
AAAGTGGGTGGATTTGAATT+TGG 0.291594 1.3:-76078160 MS.gene74966:CDS
TTTCTTCCATGAATCTCTTT+GGG 0.298282 1.3:+76079183 None:intergenic
TTTAATGTCTTTCCATGCTT+TGG 0.321669 1.3:-76078629 MS.gene74966:intron
AGCATTTCGTTCCAAATATC+AGG 0.327867 1.3:+76077732 None:intergenic
TTCAGCCACATGCATGTTCC+AGG 0.356693 1.3:-76077948 MS.gene74966:CDS
GTAATATCCTGTAGATATAC+TGG 0.367331 1.3:+76077882 None:intergenic
GGACCAGTAGTAGTGCTAAA+AGG 0.371991 1.3:-76077927 MS.gene74966:CDS
AAAGCATGGAAAGACATTAA+AGG 0.374710 1.3:+76078631 None:intergenic
TAATAAGAGAATTCTACATT+TGG 0.381121 1.3:-76078465 MS.gene74966:CDS
TTGAAGAGAATGCAAGGTAT+TGG 0.381566 1.3:-76078436 MS.gene74966:CDS
TTCTTCCAATGCAAGCCAAC+TGG 0.394214 1.3:+76079231 None:intergenic
TGCAGCCATTGGTGCCTCCC+TGG 0.399442 1.3:-76077759 MS.gene74966:CDS
TTGCATTTGATTCAGCTCAT+TGG 0.406976 1.3:-76078397 MS.gene74966:CDS
TATTCCAACTGACAGGAAAA+AGG 0.407152 1.3:-76078668 MS.gene74966:CDS
TTCTTCAGTTCTACCAATAG+TGG 0.407885 1.3:+76078497 None:intergenic
TGTATTGAGTCCTTTCTCAT+AGG 0.414458 1.3:+76078188 None:intergenic
GTTCTTCTGGGCACCACTAT+TGG 0.416633 1.3:-76078510 MS.gene74966:CDS
CATTTGATTCAGCTCATTGG+TGG 0.419213 1.3:-76078394 MS.gene74966:CDS
GACATCAATTGAGTTCTTCT+GGG 0.424420 1.3:-76078522 MS.gene74966:CDS
AGCCAACTGGACACTTCCTC+TGG 0.424797 1.3:+76079244 None:intergenic
AAGAAATGTGATTTCTCAGT+TGG 0.430768 1.3:-76079166 MS.gene74966:CDS
TTTGATGCACTGAAGTATCT+TGG 0.440296 1.3:-76078778 MS.gene74966:CDS
AAATGGAAGTGGAAGCCATT+AGG 0.444002 1.3:-76079037 MS.gene74966:CDS
AGACATTAAAGGACCATCAT+AGG 0.444756 1.3:+76078642 None:intergenic
CATTTCTCATAATCTGAATC+CGG 0.445205 1.3:+76079055 None:intergenic
CTTGGGATGGAACAACCTAA+TGG 0.446475 1.3:+76079022 None:intergenic
AGTCCTCTGATTGCAGCCAT+TGG 0.458504 1.3:-76077770 MS.gene74966:CDS
CGGATTCAGATTATGAGAAA+TGG 0.459664 1.3:-76079054 MS.gene74966:CDS
TTCTATAATGAGAAACCAAT+GGG 0.459846 1.3:-76078719 MS.gene74966:CDS
GTATGGCACCTAGACATAAC+AGG 0.470822 1.3:-76078107 MS.gene74966:intron
AGTGTCCAGTTGGCTTGCAT+TGG 0.472840 1.3:-76079236 MS.gene74966:CDS
TTCCAAATATCAGGCACACC+AGG 0.474779 1.3:+76077741 None:intergenic
TGCAAACCCAAAGAGATTCA+TGG 0.480389 1.3:-76079189 MS.gene74966:CDS
TCTCTTGTATGCTTAGTTCA+AGG 0.493508 1.3:-76078694 MS.gene74966:CDS
CATCACAAACATGAATCAAA+TGG 0.494804 1.3:-76078214 MS.gene74966:CDS
CAAATGGTTGCCTATGAGAA+AGG 0.503172 1.3:-76078198 MS.gene74966:CDS
GAGATTTCCAGTATATCTAC+AGG 0.504762 1.3:-76077889 MS.gene74966:CDS
GCAGTAACTTGTCAAAAGAA+TGG 0.505516 1.3:-76079082 MS.gene74966:CDS
GTTGGAAATGTTACAATCAA+AGG 0.507595 1.3:-76077986 MS.gene74966:CDS
TAAATTGTAGGCAAAATGGT+TGG 0.508760 1.3:-76078004 MS.gene74966:intron
ATGAGAAAGGACTCAATACA+TGG 0.509591 1.3:-76078185 MS.gene74966:CDS
TTTAGCACTACTACTGGTCC+TGG 0.509768 1.3:+76077930 None:intergenic
TATTGGTAGAACTGAAGAAA+GGG 0.514662 1.3:-76078493 MS.gene74966:CDS
AAGGAGTTATTCCAACTGAC+AGG 0.516555 1.3:-76078675 MS.gene74966:CDS
TACCAGGTGGGATTATTACA+TGG 0.524110 1.3:-76078250 MS.gene74966:intron
GCACACCAGGGAGGCACCAA+TGG 0.532334 1.3:+76077754 None:intergenic
GTTACTGCAGTGGCGAGATA+AGG 0.535012 1.3:+76079097 None:intergenic
ATTAATATCCTACCAAAGCA+TGG 0.537264 1.3:+76078617 None:intergenic
CAGATTATGAGAAATGGAAG+TGG 0.537613 1.3:-76079048 MS.gene74966:CDS
CTATTGGTAGAACTGAAGAA+AGG 0.537856 1.3:-76078494 MS.gene74966:CDS
ACATCCTTCAGTGTATAGTA+AGG 0.542161 1.3:-76077832 MS.gene74966:CDS
AGCATACAAGAGATTCCCAT+TGG 0.544133 1.3:+76078704 None:intergenic
GACTCAATACATGGGCAAAG+TGG 0.544827 1.3:-76078176 MS.gene74966:CDS
ATGACTGCATTTAGAAGAGA+TGG 0.549263 1.3:-76077855 MS.gene74966:CDS
GAGAGGCAGAAACAAGGTAA+TGG 0.549704 1.3:-76077795 MS.gene74966:CDS
ATTCTATAATGAGAAACCAA+TGG 0.552968 1.3:-76078720 MS.gene74966:CDS
CATTAGGTTGTTCCATCCCA+AGG 0.558505 1.3:-76079021 MS.gene74966:intron
AGGTAATAAGTGAAGAAGAG+AGG 0.559968 1.3:-76077812 MS.gene74966:CDS
GACAGTTGGAATCATAGAGA+GGG 0.562013 1.3:+76079119 None:intergenic
AGTACGTACCTGTTATGTCT+AGG 0.567486 1.3:+76078099 None:intergenic
GCACCAATGGCTGCAATCAG+AGG 0.571338 1.3:+76077767 None:intergenic
GTTTCATCATCACCTTGGGA+TGG 0.572029 1.3:+76079009 None:intergenic
TTGTCAAAAGAATGGACGGC+CGG 0.574038 1.3:-76079074 MS.gene74966:CDS
GGAAAGAGAATGATGTTGGT+GGG 0.575995 1.3:-76078745 MS.gene74966:CDS
ACTCAATACATGGGCAAAGT+GGG 0.582566 1.3:-76078175 MS.gene74966:CDS
GAGAGGAAAGAGAATGATGT+TGG 0.587409 1.3:-76078749 MS.gene74966:CDS
AATGGCTGCAATCAGAGGAC+TGG 0.587580 1.3:+76077772 None:intergenic
GAGAATGCAAGGTATTGGAA+AGG 0.589108 1.3:-76078431 MS.gene74966:CDS
ATCCAGAGGAAGTGTCCAGT+TGG 0.594248 1.3:-76079246 MS.gene74966:CDS
ATTTGATTGAAGAGAATGCA+AGG 0.595081 1.3:-76078442 MS.gene74966:CDS
AAGTATCTTGGTAAAATGAG+AGG 0.596417 1.3:-76078766 MS.gene74966:CDS
AGGTGGGATTATTACATGGA+AGG 0.598017 1.3:-76078246 MS.gene74966:CDS
CAATACATGGGCAAAGTGGG+TGG 0.598037 1.3:-76078172 MS.gene74966:CDS
ATTACCTTACTATACACTGA+AGG 0.598619 1.3:+76077828 None:intergenic
GTTGGAATCATAGAGAGGGT+AGG 0.612157 1.3:+76079123 None:intergenic
TAACTTGTCAAAAGAATGGA+CGG 0.614689 1.3:-76079078 MS.gene74966:CDS
GGAACGAAATGCTGAGTGCA+TGG 0.615044 1.3:-76077722 MS.gene74966:CDS
GAAGAAGAGAGGCAGAAACA+AGG 0.633633 1.3:-76077801 MS.gene74966:CDS
GGACAGTTGGAATCATAGAG+AGG 0.634353 1.3:+76079118 None:intergenic
TCCAAATATCAGGCACACCA+GGG 0.635948 1.3:+76077742 None:intergenic
AGTGGCGAGATAAGGACAGT+TGG 0.638580 1.3:+76079105 None:intergenic
AGGAAAGAGAATGATGTTGG+TGG 0.644337 1.3:-76078746 MS.gene74966:CDS
AGAAACTGAAGACGTCAACA+TGG 0.659763 1.3:-76079212 MS.gene74966:CDS
TGAGAAAGGACTCAATACAT+GGG 0.677756 1.3:-76078184 MS.gene74966:CDS
CTGGTCCTGGAACATGCATG+TGG 0.678164 1.3:+76077943 None:intergenic
ATTATCACTGATCAATCCAG+AGG 0.696073 1.3:-76079260 MS.gene74966:CDS
AAATATCAGGCACACCAGGG+AGG 0.749580 1.3:+76077745 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAATAAAATATTGATGA+AGG + chr1.3:76078979-76078998 None:intergenic 15.0%
!! AGAACATTTGATTAAATTGT+AGG - chr1.3:76079022-76079041 MS.gene74966:CDS 20.0%
!! ATAAAAATGACAGTAAATGA+AGG + chr1.3:76077733-76077752 None:intergenic 20.0%
!! TAATAAGAGAATTCTACATT+TGG - chr1.3:76078573-76078592 MS.gene74966:intron 20.0%
!!! CATTTTTAGAAGGAAAATTA+AGG + chr1.3:76078458-76078477 None:intergenic 20.0%
!!! GTAAGTAAATCATTTTTAGA+AGG + chr1.3:76078468-76078487 None:intergenic 20.0%
!!! TAATCATTTTTACATCCTAA+TGG - chr1.3:76078749-76078768 MS.gene74966:CDS 20.0%
! AAGCATGTAAGAAAACAAAA+AGG + chr1.3:76078075-76078094 None:intergenic 25.0%
! AATCAATCTTGATTAATACC+AGG - chr1.3:76078772-76078791 MS.gene74966:CDS 25.0%
! ATTCTATAATGAGAAACCAA+TGG - chr1.3:76078318-76078337 MS.gene74966:intron 25.0%
! TGATTAAATTGTAGGCAAAA+TGG - chr1.3:76079030-76079049 MS.gene74966:CDS 25.0%
! TTCTATAATGAGAAACCAAT+GGG - chr1.3:76078319-76078338 MS.gene74966:intron 25.0%
!! TAATCAAGATTGATTCCATT+AGG + chr1.3:76078767-76078786 None:intergenic 25.0%
!!! CTGTCATTTTTATTCTTCTT+AGG - chr1.3:76077740-76077759 MS.gene74966:CDS 25.0%
AAAGCATGGAAAGACATTAA+AGG + chr1.3:76078410-76078429 None:intergenic 30.0%
AAGAAATGTGATTTCTCAGT+TGG - chr1.3:76077872-76077891 MS.gene74966:CDS 30.0%
AAGTATCTTGGTAAAATGAG+AGG - chr1.3:76078272-76078291 MS.gene74966:intron 30.0%
AATCTTGATTAATACCAGGT+GGG - chr1.3:76078776-76078795 MS.gene74966:CDS 30.0%
ACATGTTGTTCACTTATTAC+AGG - chr1.3:76078486-76078505 MS.gene74966:CDS 30.0%
AGAAGCTAAATTTATCAGCA+TGG + chr1.3:76078224-76078243 None:intergenic 30.0%
ATTAATATCCTACCAAAGCA+TGG + chr1.3:76078424-76078443 None:intergenic 30.0%
ATTACCTTACTATACACTGA+AGG + chr1.3:76079213-76079232 None:intergenic 30.0%
ATTTCTTCCATGAATCTCTT+TGG + chr1.3:76077859-76077878 None:intergenic 30.0%
ATTTGATTGAAGAGAATGCA+AGG - chr1.3:76078596-76078615 MS.gene74966:intron 30.0%
CATCACAAACATGAATCAAA+TGG - chr1.3:76078824-76078843 MS.gene74966:intron 30.0%
CATTTCTCATAATCTGAATC+CGG + chr1.3:76077986-76078005 None:intergenic 30.0%
GTAATATCCTGTAGATATAC+TGG + chr1.3:76079159-76079178 None:intergenic 30.0%
GTTGGAAATGTTACAATCAA+AGG - chr1.3:76079052-76079071 MS.gene74966:CDS 30.0%
TAAATTGTAGGCAAAATGGT+TGG - chr1.3:76079034-76079053 MS.gene74966:CDS 30.0%
TAATGTTTCATCATCACCTT+GGG + chr1.3:76078036-76078055 None:intergenic 30.0%
TATTGGTAGAACTGAAGAAA+GGG - chr1.3:76078545-76078564 MS.gene74966:intron 30.0%
TTAATGTTTCATCATCACCT+TGG + chr1.3:76078037-76078056 None:intergenic 30.0%
TTTAATGTCTTTCCATGCTT+TGG - chr1.3:76078409-76078428 MS.gene74966:CDS 30.0%
TTTCTTCCATGAATCTCTTT+GGG + chr1.3:76077858-76077877 None:intergenic 30.0%
! ATGTGGCTGAAATATTTTAC+AGG + chr1.3:76079081-76079100 None:intergenic 30.0%
! TAACTTGTCAAAAGAATGGA+CGG - chr1.3:76077960-76077979 MS.gene74966:CDS 30.0%
! TGCAACATTTTTGACATGTA+TGG - chr1.3:76078099-76078118 MS.gene74966:intron 30.0%
ACATCCTTCAGTGTATAGTA+AGG - chr1.3:76079206-76079225 MS.gene74966:CDS 35.0%
AGACATCAATTGAGTTCTTC+TGG - chr1.3:76078515-76078534 MS.gene74966:CDS 35.0%
AGACATTAAAGGACCATCAT+AGG + chr1.3:76078399-76078418 None:intergenic 35.0%
AGCATTTCGTTCCAAATATC+AGG + chr1.3:76079309-76079328 None:intergenic 35.0%
AGGTAATAAGTGAAGAAGAG+AGG - chr1.3:76079226-76079245 MS.gene74966:CDS 35.0%
ATGACTGCATTTAGAAGAGA+TGG - chr1.3:76079183-76079202 MS.gene74966:CDS 35.0%
ATGAGAAAGGACTCAATACA+TGG - chr1.3:76078853-76078872 MS.gene74966:intron 35.0%
ATTATCACTGATCAATCCAG+AGG - chr1.3:76077778-76077797 MS.gene74966:CDS 35.0%
CAATCTTGATTAATACCAGG+TGG - chr1.3:76078775-76078794 MS.gene74966:CDS 35.0%
CAGATTATGAGAAATGGAAG+TGG - chr1.3:76077990-76078009 MS.gene74966:CDS 35.0%
CGGATTCAGATTATGAGAAA+TGG - chr1.3:76077984-76078003 MS.gene74966:CDS 35.0%
CTATTGGTAGAACTGAAGAA+AGG - chr1.3:76078544-76078563 MS.gene74966:intron 35.0%
GACATCAATTGAGTTCTTCT+GGG - chr1.3:76078516-76078535 MS.gene74966:CDS 35.0%
GAGATTTCCAGTATATCTAC+AGG - chr1.3:76079149-76079168 MS.gene74966:CDS 35.0%
GTGATTTCTCAGTTGGAAAA+TGG - chr1.3:76077879-76077898 MS.gene74966:CDS 35.0%
TATTCCAACTGACAGGAAAA+AGG - chr1.3:76078370-76078389 MS.gene74966:intron 35.0%
TCTCTTGTATGCTTAGTTCA+AGG - chr1.3:76078344-76078363 MS.gene74966:intron 35.0%
TGAGAAAGGACTCAATACAT+GGG - chr1.3:76078854-76078873 MS.gene74966:intron 35.0%
TGTATTGAGTCCTTTCTCAT+AGG + chr1.3:76078853-76078872 None:intergenic 35.0%
TTCTTCAGTTCTACCAATAG+TGG + chr1.3:76078544-76078563 None:intergenic 35.0%
TTGAAGAGAATGCAAGGTAT+TGG - chr1.3:76078602-76078621 MS.gene74966:intron 35.0%
TTGCATTTGATTCAGCTCAT+TGG - chr1.3:76078641-76078660 MS.gene74966:CDS 35.0%
! AAAGTGGGTGGATTTGAATT+TGG - chr1.3:76078878-76078897 MS.gene74966:intron 35.0%
! CATACTTCTAAAAACGACTC+GGG + chr1.3:76078916-76078935 None:intergenic 35.0%
! CCATACTTCTAAAAACGACT+CGG + chr1.3:76078917-76078936 None:intergenic 35.0%
! GCAGTAACTTGTCAAAAGAA+TGG - chr1.3:76077956-76077975 MS.gene74966:CDS 35.0%
! TTTGATGCACTGAAGTATCT+TGG - chr1.3:76078260-76078279 MS.gene74966:intron 35.0%
AAATGGAAGTGGAAGCCATT+AGG - chr1.3:76078001-76078020 MS.gene74966:intron 40.0%
AAGGAGTTATTCCAACTGAC+AGG - chr1.3:76078363-76078382 MS.gene74966:intron 40.0%
ACTCAATACATGGGCAAAGT+GGG - chr1.3:76078863-76078882 MS.gene74966:intron 40.0%
AGAAACTGAAGACGTCAACA+TGG - chr1.3:76077826-76077845 MS.gene74966:CDS 40.0%
AGCATACAAGAGATTCCCAT+TGG + chr1.3:76078337-76078356 None:intergenic 40.0%
AGCATGGTTTACGTGTGTTT+GGG + chr1.3:76078208-76078227 None:intergenic 40.0%
AGGAAAAAGGTCACCTATGA+TGG - chr1.3:76078383-76078402 MS.gene74966:CDS 40.0%
AGGAAAGAGAATGATGTTGG+TGG - chr1.3:76078292-76078311 MS.gene74966:intron 40.0%
AGGTGGGATTATTACATGGA+AGG - chr1.3:76078792-76078811 MS.gene74966:intron 40.0%
AGTACGTACCTGTTATGTCT+AGG + chr1.3:76078942-76078961 None:intergenic 40.0%
ATGTCTTTCCATGCTTTGGT+AGG - chr1.3:76078413-76078432 MS.gene74966:CDS 40.0%
CAAATGGTTGCCTATGAGAA+AGG - chr1.3:76078840-76078859 MS.gene74966:intron 40.0%
CATTTGATTCAGCTCATTGG+TGG - chr1.3:76078644-76078663 MS.gene74966:CDS 40.0%
GACAGTTGGAATCATAGAGA+GGG + chr1.3:76077922-76077941 None:intergenic 40.0%
GAGAGGAAAGAGAATGATGT+TGG - chr1.3:76078289-76078308 MS.gene74966:intron 40.0%
GGAAAGAGAATGATGTTGGT+GGG - chr1.3:76078293-76078312 MS.gene74966:intron 40.0%
TACCAGGTGGGATTATTACA+TGG - chr1.3:76078788-76078807 MS.gene74966:intron 40.0%
TGCAAACCCAAAGAGATTCA+TGG - chr1.3:76077849-76077868 MS.gene74966:CDS 40.0%
TTCCATGTAATAATCCCACC+TGG + chr1.3:76078793-76078812 None:intergenic 40.0%
! ACTCCTTTTAGCACTACTAC+TGG + chr1.3:76079117-76079136 None:intergenic 40.0%
! AGCTTCTCGATGACATTTTC+AGG - chr1.3:76078237-76078256 MS.gene74966:CDS 40.0%
! CTTTTGACAAGTTACTGCAG+TGG + chr1.3:76077954-76077973 None:intergenic 40.0%
!! GAGAATGCAAGGTATTGGAA+AGG - chr1.3:76078607-76078626 MS.gene74966:intron 40.0%
!!! CCGAGTCGTTTTTAGAAGTA+TGG - chr1.3:76078914-76078933 MS.gene74966:intron 40.0%
CAGCATGGTTTACGTGTGTT+TGG + chr1.3:76078209-76078228 None:intergenic 45.0%
CTTGGGATGGAACAACCTAA+TGG + chr1.3:76078019-76078038 None:intergenic 45.0%
GAAGAAGAGAGGCAGAAACA+AGG - chr1.3:76079237-76079256 MS.gene74966:CDS 45.0%
GACTCAATACATGGGCAAAG+TGG - chr1.3:76078862-76078881 MS.gene74966:intron 45.0%
GAGAGGCAGAAACAAGGTAA+TGG - chr1.3:76079243-76079262 MS.gene74966:CDS 45.0%
GGACAGTTGGAATCATAGAG+AGG + chr1.3:76077923-76077942 None:intergenic 45.0%
GGACCAGTAGTAGTGCTAAA+AGG - chr1.3:76079111-76079130 MS.gene74966:CDS 45.0%
GTATGGCACCTAGACATAAC+AGG - chr1.3:76078931-76078950 MS.gene74966:intron 45.0%
GTTGGAATCATAGAGAGGGT+AGG + chr1.3:76077918-76077937 None:intergenic 45.0%
GTTTCATCATCACCTTGGGA+TGG + chr1.3:76078032-76078051 None:intergenic 45.0%
TCCAAATATCAGGCACACCA+GGG + chr1.3:76079299-76079318 None:intergenic 45.0%
TTCCAAATATCAGGCACACC+AGG + chr1.3:76079300-76079319 None:intergenic 45.0%
TTCTTCCAATGCAAGCCAAC+TGG + chr1.3:76077810-76077829 None:intergenic 45.0%
TTGTCAAAAGAATGGACGGC+CGG - chr1.3:76077964-76077983 MS.gene74966:CDS 45.0%
TTTAGCACTACTACTGGTCC+TGG + chr1.3:76079111-76079130 None:intergenic 45.0%
! CATTAGGTTGTTCCATCCCA+AGG - chr1.3:76078017-76078036 MS.gene74966:intron 45.0%
! GTGACCTTTTTCCTGTCAGT+TGG + chr1.3:76078377-76078396 None:intergenic 45.0%
!! AAAACGACTCGGGTTTTTCG+AGG + chr1.3:76078906-76078925 None:intergenic 45.0%
AAATATCAGGCACACCAGGG+AGG + chr1.3:76079296-76079315 None:intergenic 50.0%
AATGGCTGCAATCAGAGGAC+TGG + chr1.3:76079269-76079288 None:intergenic 50.0%
AGTCCTCTGATTGCAGCCAT+TGG - chr1.3:76079268-76079287 MS.gene74966:CDS 50.0%
AGTGGCGAGATAAGGACAGT+TGG + chr1.3:76077936-76077955 None:intergenic 50.0%
CAATACATGGGCAAAGTGGG+TGG - chr1.3:76078866-76078885 MS.gene74966:intron 50.0%
GGAACGAAATGCTGAGTGCA+TGG - chr1.3:76079316-76079335 MS.gene74966:CDS 50.0%
GTTACTGCAGTGGCGAGATA+AGG + chr1.3:76077944-76077963 None:intergenic 50.0%
GTTCTTCTGGGCACCACTAT+TGG - chr1.3:76078528-76078547 MS.gene74966:CDS 50.0%
TCCCTGGTGTGCCTGATATT+TGG - chr1.3:76079295-76079314 MS.gene74966:CDS 50.0%
TTCAGCCACATGCATGTTCC+AGG - chr1.3:76079090-76079109 MS.gene74966:CDS 50.0%
!! AGTGTCCAGTTGGCTTGCAT+TGG - chr1.3:76077802-76077821 MS.gene74966:CDS 50.0%
!! ATCCAGAGGAAGTGTCCAGT+TGG - chr1.3:76077792-76077811 MS.gene74966:CDS 50.0%
!! GCTCATTGGTGGACTCATTC+TGG - chr1.3:76078655-76078674 MS.gene74966:CDS 50.0%
AGCCAACTGGACACTTCCTC+TGG + chr1.3:76077797-76077816 None:intergenic 55.0%
CTGGTCCTGGAACATGCATG+TGG + chr1.3:76079098-76079117 None:intergenic 55.0%
GCACCAATGGCTGCAATCAG+AGG + chr1.3:76079274-76079293 None:intergenic 55.0%
! TGCAGCCATTGGTGCCTCCC+TGG - chr1.3:76079279-76079298 MS.gene74966:CDS 65.0%
!! GCACACCAGGGAGGCACCAA+TGG + chr1.3:76079287-76079306 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 76077716 76079344 76077716 ID=MS.gene74966
chr1.3 mRNA 76077716 76079344 76077716 ID=MS.gene74966.t1;Parent=MS.gene74966
chr1.3 exon 76079022 76079344 76079022 ID=MS.gene74966.t1.exon1;Parent=MS.gene74966.t1
chr1.3 CDS 76079022 76079344 76079022 ID=cds.MS.gene74966.t1;Parent=MS.gene74966.t1
chr1.3 exon 76078630 76078801 76078630 ID=MS.gene74966.t1.exon2;Parent=MS.gene74966.t1
chr1.3 CDS 76078630 76078801 76078630 ID=cds.MS.gene74966.t1;Parent=MS.gene74966.t1
chr1.3 exon 76078371 76078552 76078371 ID=MS.gene74966.t1.exon3;Parent=MS.gene74966.t1
chr1.3 CDS 76078371 76078552 76078371 ID=cds.MS.gene74966.t1;Parent=MS.gene74966.t1
chr1.3 exon 76078108 76078266 76078108 ID=MS.gene74966.t1.exon4;Parent=MS.gene74966.t1
chr1.3 CDS 76078108 76078266 76078108 ID=cds.MS.gene74966.t1;Parent=MS.gene74966.t1
chr1.3 exon 76077716 76078016 76077716 ID=MS.gene74966.t1.exon5;Parent=MS.gene74966.t1
chr1.3 CDS 76077716 76078016 76077716 ID=cds.MS.gene74966.t1;Parent=MS.gene74966.t1
Gene Sequence

>MS.gene74966

ATGGCAAAAACAAACCTTCATTTACTGTCATTTTTATTCTTCTTAGGCTTATTCAACTGCACATTATCACTGATCAATCCAGAGGAAGTGTCCAGTTGGCTTGCATTGGAAGAAACTGAAGACGTCAACATGGTGCAAACCCAAAGAGATTCATGGAAGAAATGTGATTTCTCAGTTGGAAAATGGATATTTGATCAAACCTACCCTCTCTATGATTCCAACTGTCCTTATCTCGCCACTGCAGTAACTTGTCAAAAGAATGGACGGCCGGATTCAGATTATGAGAAATGGAAGTGGAAGCCATTAGGTTGTTCCATCCCAAGGTGATGATGAAACATTAATTGCTCACATTGCTACCTTTTTGTTTTCTTACATGCTTGCATTGCAACATTTTTGACATGTATGGATGTTCTCTACAATTGATTTTTAGTCGACTTTTATGTAAATGTATTCAAACATAAATCACTTTACATTTAACTCACTTTTAGTCCCAAACACACGTAAACCATGCTGATAAATTTAGCTTCTCGATGACATTTTCAGGTTTGATGCACTGAAGTATCTTGGTAAAATGAGAGGAAAGAGAATGATGTTGGTGGGCGATTCTATAATGAGAAACCAATGGGAATCTCTTGTATGCTTAGTTCAAGGAGTTATTCCAACTGACAGGAAAAAGGTCACCTATGATGGTCCTTTAATGTCTTTCCATGCTTTGGTAGGATATTAATCAATCTTATATCCTTAATTTTCCTTCTAAAAATGATTTACTTACATGTTGTTCACTTATTACAGGATTTTGAGACATCAATTGAGTTCTTCTGGGCACCACTATTGGTAGAACTGAAGAAAGGGTCTAATAATAAGAGAATTCTACATTTGGATTTGATTGAAGAGAATGCAAGGTATTGGAAAGGAGTTGATATTCTTGCATTTGATTCAGCTCATTGGTGGACTCATTCTGGTCAATCTAGCTCGTAAGAAACATTTCTCATTTTTTAGTTTTGCACTTAATTGAAAGTGTTACATTTGAAGATAATCATTTTTACATCCTAATGGAATCAATCTTGATTAATACCAGGTGGGATTATTACATGGAAGGAAATAGTATCATCACAAACATGAATCAAATGGTTGCCTATGAGAAAGGACTCAATACATGGGCAAAGTGGGTGGATTTGAATTTGGATCCTCGAAAAACCCGAGTCGTTTTTAGAAGTATGGCACCTAGACATAACAGGTACGTACTATCAATTTTTATTTCCTTCATCAATATTTTATTTTTGCTTTAGTCTCAATAACATATTTAAGAACATTTGATTAAATTGTAGGCAAAATGGTTGGAAATGTTACAATCAAAGGCAACCTGTAAAATATTTCAGCCACATGCATGTTCCAGGACCAGTAGTAGTGCTAAAAGGAGTGCTGAAGAGAATGAGATTTCCAGTATATCTACAGGATATTACAACAATGACTGCATTTAGAAGAGATGGACATCCTTCAGTGTATAGTAAGGTAATAAGTGAAGAAGAGAGGCAGAAACAAGGTAATGGCCAGTCCTCTGATTGCAGCCATTGGTGCCTCCCTGGTGTGCCTGATATTTGGAACGAAATGCTGAGTGCATGGCTTTAA
Protein sequence

>MS.gene74966.t1

MAKTNLHLLSFLFFLGLFNCTLSLINPEEVSSWLALEETEDVNMVQTQRDSWKKCDFSVGKWIFDQTYPLYDSNCPYLATAVTCQKNGRPDSDYEKWKWKPLGCSIPRFDALKYLGKMRGKRMMLVGDSIMRNQWESLVCLVQGVIPTDRKKVTYDGPLMSFHALDFETSIEFFWAPLLVELKKGSNNKRILHLDLIEENARYWKGVDILAFDSAHWWTHSGQSSSWDYYMEGNSIITNMNQMVAYEKGLNTWAKWVDLNLDPRKTRVVFRSMAPRHNRQNGWKCYNQRQPVKYFSHMHVPGPVVVLKGVLKRMRFPVYLQDITTMTAFRRDGHPSVYSKVISEEERQKQGNGQSSDCSHWCLPGVPDIWNEMLSAWL